RLG00000018563

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015813
Physical Location & Seq
Forward (+)
31969344 .. 31975693
6350 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018563

Sequence Viewer

Length: 1455 bp
ATGGTACAGAGAGGCTATCACAGACCCAGACCACCATCTCCGCACGCCATTAAACTCAACCTAATCCTCATTTTCGCCTTCTTCTTTTTGGTCTTCATCTTCTACACAAGGCAATGGCATCTGCTCGACGATCAGCAAAAGCAACAGCCTTTCTTCGATGAACATTCCCAAACTGACCGGATGCCAGATGCAGAACTTTGGGATGCTCCTCTTAGCTATGGTTTACGCTCATGTGTCAAGCCGACTTCTAGATATAAAGCTGCCACTGGATGGGACCACTACATGACTGTGAGAAGTAATGGAGGACTAAATCAGATGCGAACTGGCGTATCCTCTATACTCTCTATGGTATCAGTACAAACAATAATTTGCTTAGAAATACTTACATTTTCAGATATATTTGATGAACTTCACTTCATTGAAACTCTGCAAGAAGATGTCAAGATTGTCAAGGAGCTCCCACAGGAATTAGCAAATGTTCCTCGGGCGAGGAAGCATTTCACATCCTGGGCTACCACCAGTTACTATGAAGACATGACACAGCTATGGAAAGAATATCAGGTGATACATGTTGCAAAATCAGATTCTCGACTTGCAAACAATGATCTTCCTCTTGATATCCAGAGGTTGAGATGCCGTGCCTTGTATCAGGCGCTCCGTTTCTCTCCCCCAATTGAGAGCTTTGGAAAGAAGCTGGTGGAGCGGCTTAGAGCACGTGGGGGAAAATATATTGCTCTTCATTTGAGATATGAGAAAGACATGTTGTCTTTTACTGGTTGTTCTTATGGTTTGACTGATGAGGAATCCGAGGAACTAAGAGTAATGAGGGAGAACACAAATCACTGGAAAGTTAAAAAGATTAACTCTACTGAGCAGAGACTAGGTGGCTTCTGTCCACTAACTCCCAAGGAGATTGGCATATTTCTTCGAGCTCTCGGTTATCCACCATCATCATTAATATATATTGCAGCTGGGGAGATCTATGGTGGTAGTACTCACCTTTTGGAGCTCACATCCCGTTTTCCAAATATAGTTTTCAAGGAAACACTTGCGACGCAGGAGGAACTAAAGGCATTTACTAATCATGCATCTCAAACTGCAGCTCTTGATTATGTGATCTCAGTTGAAAGTGATGTGTTTGTCCCATCATATTCTGGTAACATGGCAAGAGCTGTTGAGGGCCAACGCAGATTCTTAGGTCACCGCAAAACAATCACTCCAGATAGGAAAGGACTTGTTGAAGTTTTTGATATGCTGGAAAATGGACAACAAACAGAAGGATCTTCGCTGTCACATATTATACAGACCATGCACAAAAATAGGAAAGGAGCTCCAAGGAGGAGAAGAGGGCCACTGCCAGAAATGAAAGGTCGAGCACGGTTGAGAACTGAAGAATCTTTCTATGAAAACCCTTACCCTGAGTGTATATGTGCTTTGAAAAAGCACTTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

485

Amino Acids

55.78

Weight (kDa)

9.03

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 94 - 398 1.7e-83 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64600
fragaria_vesca FvH4_1g24200 FvH4_1g24200 FvH4_1g24200
rosa_chinensis RchiOBHm_Chr2g0120631 RchiOBHm_Chr2g0120641
rosa_laevigata RLG00000018563
rosa_multiflora Rmu_sc0001844.1_g000005
rosa_roxburghii Rroxscaffold_2G00123270
rosa_rugosa Rorug02G0230000
rosa_samantha Rh2AG286000 Rh2BG297800 Rh2CG275500 Rh2DG311500
rosa_wichuraiana Rw2G022970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 703
AciI CCGC 3 cut(s) 41, 703, 1204
AclWI GGATC 1 cut(s) 1288
AcuI CTGAAG 1 cut(s) 1410
AcvI CACGTG 1 cut(s) 716
AfaI GTAC 3 cut(s) 6, 357, 994
AfiI CCNNNNNNNGG 1 cut(s) 270
AflIII ACRYGT 2 cut(s) 568, 759
AgsI TTSAA 5 cut(s) 422, 1039, 1127, 1241, 1438
AhdI GACNNNNNGTC 1 cut(s) 763
AjnI CCWGG 1 cut(s) 506
AjuI GAANNNNNNNTTGG 1 cut(s) 1430
Alw21I GWGCWC 6 cut(s) 459, 715, 934, 1011, 1333, 1378
Alw26I GTCTC 1 cut(s) 871
AlwI GGATC 1 cut(s) 1288
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 2 cut(s) 1180, 1349
ApeKI GCWGC 3 cut(s) 260, 968, 1100
AseI ATTAAT 1 cut(s) 956
Asp700I GAANNNNTTC 1 cut(s) 497
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 3 cut(s) 274, 1180, 1349
AsuHPI GGTGA 3 cut(s) 574, 989, 1193
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 1 cut(s) 274
BaeI ACNNNNGTAYC 2 cut(s) 312, 345
BanII GRGCYC 4 cut(s) 459, 934, 1011, 1333
BbrPI CACGTG 1 cut(s) 716
BbsI GAAGAC 2 cut(s) 85, 537
Bbv12I GWGCWC 6 cut(s) 459, 715, 934, 1011, 1333, 1378
BbvI GCAGC 3 cut(s) 247, 980, 1112
BccI CCATC 4 cut(s) 43, 264, 955, 1153
BceAI ACGGC 1 cut(s) 621
BciT130I CCWGG 1 cut(s) 508
BciVI GTATCC 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 871
BfaI CTAG 2 cut(s) 249, 881
BfmI CTRYAG 1 cut(s) 1098
BfoI RGCGCY 1 cut(s) 656
BfuI GTATCC 1 cut(s) 340
BglII AGATCT 1 cut(s) 978
BisI GCNGC 4 cut(s) 261, 704, 969, 1101
BlsI GCNGC 4 cut(s) 262, 705, 970, 1102
BmcAI AGTACT 1 cut(s) 994
Bme1390I CCNGG 1 cut(s) 508
Bme18I GGWCC 1 cut(s) 274
BmeRI GACNNNNNGTC 1 cut(s) 763
BmeT110I CYCGRG 1 cut(s) 483
BmgT120I GGNCC 3 cut(s) 274, 1180, 1349
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 1 cut(s) 508
BmsI GCATC 7 cut(s) 127, 171, 178, 193, 306, 623, 1097
BpiI GAAGAC 2 cut(s) 85, 537
BpmI CTGGAG 1 cut(s) 1203
BsaAI YACGTR 1 cut(s) 716
BsaJI CCNNGG 5 cut(s) 482, 507, 807, 906, 1334
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 1201, 1231
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse1I ACTGG 5 cut(s) 271, 328, 519, 778, 848
Bse3DI GCAATG 1 cut(s) 119
BseBI CCWGG 1 cut(s) 508
BseDI CCNNGG 5 cut(s) 482, 507, 807, 906, 1334
BseGI GGATG 5 cut(s) 186, 208, 275, 503, 1013
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMI GCAATG 1 cut(s) 119
BseMII CTCAG 3 cut(s) 861, 1134, 1410
BseNI ACTGG 5 cut(s) 271, 328, 519, 778, 848
BseRI GAGGAG 2 cut(s) 198, 1354
BseXI GCAGC 3 cut(s) 247, 980, 1112
BseYI CCCAGC 1 cut(s) 971
BshFI GGCC 2 cut(s) 1182, 1351
BsiHKAI GWGCWC 6 cut(s) 459, 715, 934, 1011, 1333, 1378
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 2 cut(s) 287, 1127
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 871
BsmFI GGGAC 2 cut(s) 287, 1127
BsnI GGCC 2 cut(s) 1182, 1351
BsoBI CYCGRG 1 cut(s) 483
Bsp1286I GDGCHC 6 cut(s) 459, 715, 934, 1011, 1333, 1378
Bsp143I GATC 5 cut(s) 130, 604, 978, 1116, 1280
BspACI CCGC 3 cut(s) 41, 703, 1204
BspANI GGCC 2 cut(s) 1182, 1351
BspCNI CTCAG 3 cut(s) 862, 1133, 1411
BspLI GGNNCC 1 cut(s) 275
BspMAI CTGCAG 1 cut(s) 1102
BspPI GGATC 1 cut(s) 1288
BspQI GCTCTTC 1 cut(s) 741
BsrBI CCGCTC 1 cut(s) 703
BsrDI GCAATG 1 cut(s) 119
BsrI ACTGG 5 cut(s) 271, 328, 519, 778, 848
BssECI CCNNGG 5 cut(s) 482, 507, 807, 906, 1334
BssMI GATC 5 cut(s) 130, 604, 978, 1116, 1280
BssT1I CCWWGG 2 cut(s) 906, 1334
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 2 cut(s) 289, 1380
Bst6I CTCTTC 2 cut(s) 741, 1339
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 8 cut(s) 212, 373, 707, 815, 870, 1120, 1195, 1419
BstEII GGTNACC 1 cut(s) 1199
BstF5I GGATG 5 cut(s) 186, 208, 275, 503, 1013
BstH2I RGCGCY 1 cut(s) 656
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 5 cut(s) 133, 607, 981, 1119, 1283
BstMAI GTCTC 1 cut(s) 871
BstMBI GATC 5 cut(s) 130, 604, 978, 1116, 1280
BstMWI GCNNNNNNNGC 1 cut(s) 700
BstNI CCWGG 1 cut(s) 508
BstNSI RCATGY 2 cut(s) 572, 763
BstPI GGTNACC 1 cut(s) 1199
BstSCI CCNGG 1 cut(s) 506
BstSFI CTRYAG 1 cut(s) 1098
BstV1I GCAGC 3 cut(s) 247, 980, 1112
BstV2I GAAGAC 2 cut(s) 85, 537
BstX2I RGATCY 2 cut(s) 978, 1280
BstYI RGATCY 2 cut(s) 978, 1280
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 2 cut(s) 1182, 1351
BtsCI GGATG 5 cut(s) 186, 208, 275, 503, 1013
BtsI GCAGTG 1 cut(s) 1352
BtsIMutI CAGTG 3 cut(s) 264, 841, 1352
Cac8I GCNNGC 1 cut(s) 45
CfoI GCGC 1 cut(s) 655
Cfr13I GGNCC 3 cut(s) 274, 1180, 1349
CseI GACGC 1 cut(s) 1063
Csp6I GTAC 3 cut(s) 5, 356, 993
CviAII CATG 8 cut(s) 231, 283, 535, 569, 760, 1085, 1162, 1309
CviQI GTAC 3 cut(s) 5, 356, 993
DdeI CTNAG 8 cut(s) 212, 373, 707, 815, 870, 1120, 1195, 1419
DpnI GATC 5 cut(s) 132, 606, 980, 1118, 1282
DpnII GATC 5 cut(s) 130, 604, 978, 1116, 1280
DriI GACNNNNNGTC 1 cut(s) 763
Eam1104I CTCTTC 2 cut(s) 741, 1339
Eam1105I GACNNNNNGTC 1 cut(s) 763
EarI CTCTTC 2 cut(s) 741, 1339
Ecl136II GAGCTC 4 cut(s) 457, 932, 1009, 1331
Eco130I CCWWGG 2 cut(s) 906, 1334
Eco24I GRGCYC 4 cut(s) 459, 934, 1011, 1333
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 4 cut(s) 457, 932, 1009, 1331
Eco57I CTGAAG 1 cut(s) 1410
Eco72I CACGTG 1 cut(s) 716
Eco88I CYCGRG 1 cut(s) 483
Eco91I GGTNACC 1 cut(s) 1199
EcoICRI GAGCTC 4 cut(s) 457, 932, 1009, 1331
EcoO65I GGTNACC 1 cut(s) 1199
EcoRII CCWGG 1 cut(s) 506
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 2 cut(s) 906, 1334
EcoT22I ATGCAT 1 cut(s) 1090
EcoT38I GRGCYC 4 cut(s) 459, 934, 1011, 1333
ErhI CCWWGG 2 cut(s) 906, 1334
FaeI CATG 8 cut(s) 234, 286, 538, 572, 763, 1088, 1165, 1312
FaqI GGGAC 2 cut(s) 287, 1127
FatI CATG 8 cut(s) 230, 282, 534, 568, 759, 1084, 1161, 1308
Fnu4HI GCNGC 4 cut(s) 261, 704, 969, 1101
FokI GGATG 5 cut(s) 193, 215, 282, 490, 1000
FriOI GRGCYC 4 cut(s) 459, 934, 1011, 1333
Fsp4HI GCNGC 4 cut(s) 261, 704, 969, 1101
FspBI CTAG 2 cut(s) 249, 881
GlaI GCGC 1 cut(s) 654
GluI GCNGC 4 cut(s) 261, 704, 969, 1101
GsaI CCCAGC 1 cut(s) 975
GsuI CTGGAG 1 cut(s) 1203
HaeII RGCGCY 1 cut(s) 656
HaeIII GGCC 2 cut(s) 1182, 1351
HapII CCGG 1 cut(s) 178
HgaI GACGC 1 cut(s) 1063
HhaI GCGC 1 cut(s) 655
Hin1II CATG 8 cut(s) 234, 286, 538, 572, 763, 1088, 1165, 1312
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HinfI GANTC 4 cut(s) 584, 803, 1191, 1394
HpaII CCGG 1 cut(s) 178
HphI GGTGA 3 cut(s) 574, 989, 1193
Hpy166II GTNNAC 2 cut(s) 224, 896
Hpy188I TCNGA 4 cut(s) 315, 394, 583, 808
Hpy188III TCNNGA 7 cut(s) 249, 442, 588, 614, 622, 1106, 1220
Hpy8I GTNNAC 2 cut(s) 224, 896
Hpy99I CGWCG 2 cut(s) 131, 1057
HpyAV CCTTC 2 cut(s) 88, 1271
HpyCH4III ACNGT 2 cut(s) 289, 1380
HpyCH4IV ACGT 1 cut(s) 715
HpyCH4V TGCA 8 cut(s) 191, 430, 575, 596, 968, 1088, 1100, 1312
HpyF10VI GCNNNNNNNGC 1 cut(s) 700
HpyF3I CTNAG 8 cut(s) 212, 373, 707, 815, 870, 1120, 1195, 1419
HpySE526I ACGT 1 cut(s) 715
Hsp92II CATG 8 cut(s) 234, 286, 538, 572, 763, 1088, 1165, 1312
HspAI GCGC 1 cut(s) 653
Kzo9I GATC 5 cut(s) 130, 604, 978, 1116, 1280
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 8 cut(s) 211, 454, 462, 660, 700, 1006, 1328, 1336
Lsp1109I GCAGC 3 cut(s) 247, 980, 1112
LweI GCATC 7 cut(s) 127, 171, 178, 193, 306, 623, 1097
MaeI CTAG 2 cut(s) 249, 881
MaeII ACGT 1 cut(s) 715
MaeIII GTNAC 4 cut(s) 521, 1157, 1199, 1290
MalI GATC 5 cut(s) 132, 606, 980, 1118, 1282
MbiI CCGCTC 1 cut(s) 703
MboI GATC 5 cut(s) 130, 604, 978, 1116, 1280
MfeI CAATTG 1 cut(s) 672
MflI RGATCY 2 cut(s) 978, 1280
MhlI GDGCHC 6 cut(s) 459, 715, 934, 1011, 1333, 1378
MluCI AATT 3 cut(s) 366, 467, 672
Mph1103I ATGCAT 1 cut(s) 1090
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 4 cut(s) 51, 852, 861, 956
MslI CAYNNNNRTG 2 cut(s) 287, 544
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 508
MunI CAATTG 1 cut(s) 672
MvaI CCWGG 1 cut(s) 508
MwoI GCNNNNNNNGC 1 cut(s) 700
NdeII GATC 5 cut(s) 130, 604, 978, 1116, 1280
NlaIII CATG 8 cut(s) 234, 286, 538, 572, 763, 1088, 1165, 1312
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 2 cut(s) 1199, 1290
NsiI ATGCAT 1 cut(s) 1090
NspI RCATGY 2 cut(s) 572, 763
PciI ACATGT 2 cut(s) 568, 759
PciSI GCTCTTC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 4 cut(s) 584, 803, 1191, 1394
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 4 cut(s) 262, 705, 970, 1102
PmaCI CACGTG 1 cut(s) 716
PmlI CACGTG 1 cut(s) 716
Ppu21I YACGTR 1 cut(s) 716
PscI ACATGT 2 cut(s) 568, 759
PshBI ATTAAT 1 cut(s) 956
Psp124BI GAGCTC 4 cut(s) 459, 934, 1011, 1333
Psp6I CCWGG 1 cut(s) 506
PspCI CACGTG 1 cut(s) 716
PspEI GGTNACC 1 cut(s) 1199
PspFI CCCAGC 1 cut(s) 971
PspGI CCWGG 1 cut(s) 506
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 3 cut(s) 274, 1180, 1349
PstI CTGCAG 1 cut(s) 1102
PsuI RGATCY 2 cut(s) 978, 1280
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 3 cut(s) 6, 357, 994
RsaNI GTAC 3 cut(s) 5, 356, 993
RseI CAYNNNNRTG 2 cut(s) 287, 544
SacI GAGCTC 4 cut(s) 459, 934, 1011, 1333
SapI GCTCTTC 1 cut(s) 741
SaqAI TTAA 4 cut(s) 51, 852, 861, 956
SatI GCNGC 4 cut(s) 261, 704, 969, 1101
Sau3AI GATC 5 cut(s) 130, 604, 978, 1116, 1280
Sau96I GGNCC 3 cut(s) 274, 1180, 1349
ScaI AGTACT 1 cut(s) 994
ScrFI CCNGG 1 cut(s) 508
SduI GDGCHC 6 cut(s) 459, 715, 934, 1011, 1333, 1378
SfaNI GCATC 7 cut(s) 127, 171, 178, 193, 306, 623, 1097
SfcI CTRYAG 1 cut(s) 1098
SinI GGWCC 1 cut(s) 274
SmiMI CAYNNNNRTG 2 cut(s) 287, 544
Sse9I AATT 3 cut(s) 366, 467, 672
SsiI CCGC 3 cut(s) 41, 703, 1204
SspMI CTAG 2 cut(s) 249, 881
SstI GAGCTC 4 cut(s) 459, 934, 1011, 1333
StyD4I CCNGG 1 cut(s) 506
StyI CCWWGG 2 cut(s) 906, 1334
TaaI ACNGT 2 cut(s) 289, 1380
TaiI ACGT 1 cut(s) 718
TaqI TCGA 5 cut(s) 126, 156, 589, 928, 1372
TasI AATT 3 cut(s) 366, 467, 672
TatI WGTACW 2 cut(s) 355, 992
TauI GCSGC 1 cut(s) 706
TfiI GAWTC 4 cut(s) 584, 803, 1191, 1394
Tru1I TTAA 4 cut(s) 51, 852, 861, 956
Tru9I TTAA 4 cut(s) 51, 852, 861, 956
TscAI CASTG 3 cut(s) 271, 848, 1359
TseFI GTSAC 2 cut(s) 1199, 1290
TseI GCWGC 3 cut(s) 260, 968, 1100
Tsp45I GTSAC 2 cut(s) 1199, 1290
TspDTI ATGAA 8 cut(s) 85, 174, 406, 420, 543, 728, 1379, 1419
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 3 cut(s) 271, 848, 1359
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 274
VspI ATTAAT 1 cut(s) 956
XbaI TCTAGA 1 cut(s) 248
XceI RCATGY 2 cut(s) 572, 763
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 2 cut(s) 249, 881
ZrmI AGTACT 1 cut(s) 994
Zsp2I ATGCAT 1 cut(s) 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.