Rh2DG311500

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015813
Physical Location & Seq
Forward (+)
38244697 .. 38251211
6515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG311500.1

Sequence Viewer

Length: 1128 bp
ATGGTACAGAGAGGCTATCACAGACCCAGACCACCATCTCCACACGCCATTAAACTCAACCTAATCCTCATTTTCGCCTTCTTCTTTTTGGTCTTCATCTTCTACACAAGGCAATGGCATCTGCTCGACGATCAGCAAAAGCAACAGCCTTTCTCCGATGAACATTCCCAAACTGACCGGATGCCAGATGCAGAACTTTGGGATGCTCCTCCTAGCTATGGTTTACGCTCATGTGTCAAGCCGACCTCTAGATATAAAGCTGCCACTGGATGGGACCACTACATGACTGTGAGGAGTAATGGAGGACTAAATCAGATGCGAACAGGCATTGCAGACATGGTGGCTGTGGCACGCATTATGAATGCGACCTTGGTCATTCCTCAATTGGATAAACGTTCATTCTGGCATGACTCGAGTACATTTTCAGATATATTTGATGAACTTCACTTCATTGAAACTCTGCAAGAAGATGTCAAGATTGTCAAGGAGCTCCCACAGGAATTGGCAAATGTTCCTCGGGCGAGGAAGCATTTCACATCCTGGGCTAGCACCAGTTACTATGAAGACATGACACAGCTATGGAAAGAATATCAGGTGATTCATGTTGCAAAATCAGATTCTCGACTTGCAAACAATGATCTCCCTCTTGATATCCAGAGGTTGAGATGCCGTGCCTTGTATCATGCGCTCCGTTTCTCTCCCCCAATTGAGAGCTTTGGAAAGAAGCTGGTGGAGCGGCTTAGAGCACGTGGGGGAAAATATATTGCTCTTCATTTGAGATATGAGAAAGACATGTTGTCTTTTACTGGTTGTTCTTATGGTTTGACTGATGAGGAATCCGAGGAACTAAGAATAATGAGGGAGAACACAAATCACTGGAAAGTTAAAAAGATTAACTCTACTGAGCAGAGACTAGGTGGCTTCTGTCCACTAACTCCCAAGGAGATTGGCATATTTCTTCGAGCTCTCGGTTATCCACCATCATCGTTAATATATATTGCAGCTGGGGAGATCTATGGTGGTAGTACTCACCTTTTGGAGCTCACATCCCGTTTTCCAAATATAGTTTTCAAGGTTGGTCAGTTTCGTAGTTTTTTTTTTTTGTATTGCCAAAGTAGTTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

375

Amino Acids

43.57

Weight (kDa)

9.03

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 94 - 358 4.1e-85 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64600
fragaria_vesca FvH4_1g24200 FvH4_1g24200 FvH4_1g24200
rosa_chinensis RchiOBHm_Chr2g0120631 RchiOBHm_Chr2g0120641
rosa_laevigata RLG00000018563
rosa_multiflora Rmu_sc0001844.1_g000005
rosa_roxburghii Rroxscaffold_2G00123270
rosa_rugosa Rorug02G0230000
rosa_samantha Rh2AG286000 Rh2BG297800 Rh2CG275500 Rh2DG311500
rosa_wichuraiana Rw2G022970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 736
AciI CCGC 1 cut(s) 736
AclI AACGTT 1 cut(s) 394
AcvI CACGTG 1 cut(s) 749
AfaI GTAC 3 cut(s) 6, 418, 1027
AfiI CCNNNNNNNGG 2 cut(s) 218, 270
AflIII ACRYGT 1 cut(s) 792
AgsI TTSAA 2 cut(s) 455, 1072
AhdI GACNNNNNGTC 1 cut(s) 796
AjnI CCWGG 1 cut(s) 539
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AluBI AGCT 9 cut(s) 216, 260, 490, 577, 714, 727, 965, 1004, 1042
AluI AGCT 9 cut(s) 216, 260, 490, 577, 714, 727, 965, 1004, 1042
Alw21I GWGCWC 4 cut(s) 492, 748, 967, 1044
Alw26I GTCTC 1 cut(s) 904
Ama87I CYCGRG 2 cut(s) 412, 516
ApeKI GCWGC 2 cut(s) 260, 1001
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 688
AspS9I GGNCC 1 cut(s) 274
AsuHPI GGTGA 2 cut(s) 607, 1022
AsuNHI GCTAGC 1 cut(s) 545
AvaI CYCGRG 2 cut(s) 412, 516
AvaII GGWCC 1 cut(s) 274
BanII GRGCYC 3 cut(s) 492, 967, 1044
BbrPI CACGTG 1 cut(s) 749
BbsI GAAGAC 2 cut(s) 85, 570
Bbv12I GWGCWC 4 cut(s) 492, 748, 967, 1044
BbvI GCAGC 2 cut(s) 247, 1013
BccI CCATC 3 cut(s) 43, 264, 988
BceAI ACGGC 1 cut(s) 654
BciT130I CCWGG 1 cut(s) 541
BcoDI GTCTC 1 cut(s) 904
BfaI CTAG 4 cut(s) 213, 249, 546, 914
BglII AGATCT 1 cut(s) 1011
BisI GCNGC 3 cut(s) 261, 737, 1002
BlsI GCNGC 3 cut(s) 262, 738, 1003
BmcAI AGTACT 1 cut(s) 1027
Bme1390I CCNGG 1 cut(s) 541
Bme18I GGWCC 1 cut(s) 274
BmeRI GACNNNNNGTC 1 cut(s) 796
BmeT110I CYCGRG 2 cut(s) 412, 516
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 1 cut(s) 541
BmsI GCATC 6 cut(s) 127, 171, 178, 193, 306, 656
BmtI GCTAGC 1 cut(s) 549
BoxI GACNNNNGTC 1 cut(s) 371
BpiI GAAGAC 2 cut(s) 85, 570
BsaAI YACGTR 1 cut(s) 749
BsaJI CCNNGG 5 cut(s) 369, 515, 540, 840, 939
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 270
Bse1I ACTGG 4 cut(s) 271, 552, 811, 881
Bse3DI GCAATG 2 cut(s) 119, 327
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 5 cut(s) 369, 515, 540, 840, 939
BseGI GGATG 5 cut(s) 186, 208, 275, 536, 1046
BseLI CCNNNNNNNGG 2 cut(s) 218, 270
BseMI GCAATG 2 cut(s) 119, 327
BseMII CTCAG 1 cut(s) 894
BseNI ACTGG 4 cut(s) 271, 552, 811, 881
BseRI GAGGAG 2 cut(s) 198, 307
BseXI GCAGC 2 cut(s) 247, 1013
BseYI CCCAGC 1 cut(s) 1004
BsiHKAI GWGCWC 4 cut(s) 492, 748, 967, 1044
BsiHKCI CYCGRG 2 cut(s) 412, 516
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 2 cut(s) 218, 270
BsmAI GTCTC 1 cut(s) 904
BsmFI GGGAC 1 cut(s) 287
BsmI GAATGC 1 cut(s) 367
BsoBI CYCGRG 2 cut(s) 412, 516
Bsp1286I GDGCHC 4 cut(s) 492, 748, 967, 1044
Bsp143I GATC 3 cut(s) 130, 637, 1011
BspACI CCGC 1 cut(s) 736
BspCNI CTCAG 1 cut(s) 895
BspLI GGNNCC 1 cut(s) 275
BspOI GCTAGC 1 cut(s) 549
BspQI GCTCTTC 1 cut(s) 774
BsrBI CCGCTC 1 cut(s) 736
BsrDI GCAATG 2 cut(s) 119, 327
BsrI ACTGG 4 cut(s) 271, 552, 811, 881
BssECI CCNNGG 5 cut(s) 369, 515, 540, 840, 939
BssMI GATC 3 cut(s) 130, 637, 1011
BssT1I CCWWGG 2 cut(s) 369, 939
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 774
BstBAI YACGTR 1 cut(s) 749
BstC8I GCNNGC 2 cut(s) 352, 547
BstDEI CTNAG 3 cut(s) 740, 848, 903
BstF5I GGATG 5 cut(s) 186, 208, 275, 536, 1046
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 3 cut(s) 133, 640, 1014
BstMAI GTCTC 1 cut(s) 904
BstMBI GATC 3 cut(s) 130, 637, 1011
BstMWI GCNNNNNNNGC 1 cut(s) 733
BstNI CCWGG 1 cut(s) 541
BstNSI RCATGY 1 cut(s) 796
BstPAI GACNNNNGTC 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 539
BstV1I GCAGC 2 cut(s) 247, 1013
BstV2I GAAGAC 2 cut(s) 85, 570
BstX2I RGATCY 1 cut(s) 1011
BstYI RGATCY 1 cut(s) 1011
BtsCI GGATG 5 cut(s) 186, 208, 275, 536, 1046
BtsIMutI CAGTG 2 cut(s) 264, 874
Cac8I GCNNGC 2 cut(s) 352, 547
CfoI GCGC 1 cut(s) 688
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 3 cut(s) 5, 417, 1026
CspCI CAANNNNNGTGG 2 cut(s) 483, 518
CviAII CATG 8 cut(s) 231, 283, 337, 407, 568, 602, 683, 793
CviQI GTAC 3 cut(s) 5, 417, 1026
DdeI CTNAG 3 cut(s) 740, 848, 903
DpnI GATC 3 cut(s) 132, 639, 1013
DpnII GATC 3 cut(s) 130, 637, 1011
DriI GACNNNNNGTC 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 774
Eam1105I GACNNNNNGTC 1 cut(s) 796
EarI CTCTTC 1 cut(s) 774
Ecl136II GAGCTC 3 cut(s) 490, 965, 1042
Eco130I CCWWGG 2 cut(s) 369, 939
Eco24I GRGCYC 3 cut(s) 492, 967, 1044
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 3 cut(s) 490, 965, 1042
Eco72I CACGTG 1 cut(s) 749
Eco88I CYCGRG 2 cut(s) 412, 516
EcoICRI GAGCTC 3 cut(s) 490, 965, 1042
EcoRII CCWGG 1 cut(s) 539
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 2 cut(s) 369, 939
EcoT38I GRGCYC 3 cut(s) 492, 967, 1044
ErhI CCWWGG 2 cut(s) 369, 939
FaeI CATG 8 cut(s) 234, 286, 340, 410, 571, 605, 686, 796
FaqI GGGAC 1 cut(s) 287
FatI CATG 8 cut(s) 230, 282, 336, 406, 567, 601, 682, 792
Fnu4HI GCNGC 3 cut(s) 261, 737, 1002
FokI GGATG 5 cut(s) 193, 215, 282, 523, 1033
FriOI GRGCYC 3 cut(s) 492, 967, 1044
Fsp4HI GCNGC 3 cut(s) 261, 737, 1002
FspBI CTAG 4 cut(s) 213, 249, 546, 914
GlaI GCGC 1 cut(s) 687
GluI GCNGC 3 cut(s) 261, 737, 1002
GsaI CCCAGC 1 cut(s) 1008
HapII CCGG 1 cut(s) 178
HhaI GCGC 1 cut(s) 688
Hin1II CATG 8 cut(s) 234, 286, 340, 410, 571, 605, 686, 796
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HinfI GANTC 4 cut(s) 410, 598, 617, 836
HpaII CCGG 1 cut(s) 178
HphI GGTGA 2 cut(s) 607, 1022
Hpy166II GTNNAC 2 cut(s) 224, 929
Hpy188I TCNGA 5 cut(s) 157, 315, 427, 616, 841
Hpy188III TCNNGA 5 cut(s) 249, 475, 621, 647, 655
Hpy8I GTNNAC 2 cut(s) 224, 929
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 394, 748
HpyCH4V TGCA 6 cut(s) 191, 332, 463, 608, 629, 1001
HpyF10VI GCNNNNNNNGC 1 cut(s) 733
HpyF3I CTNAG 3 cut(s) 740, 848, 903
HpySE526I ACGT 2 cut(s) 394, 748
Hsp92II CATG 8 cut(s) 234, 286, 340, 410, 571, 605, 686, 796
HspAI GCGC 1 cut(s) 686
Kzo9I GATC 3 cut(s) 130, 637, 1011
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 6 cut(s) 211, 487, 495, 693, 733, 1039
Lsp1109I GCAGC 2 cut(s) 247, 1013
LweI GCATC 6 cut(s) 127, 171, 178, 193, 306, 656
MaeI CTAG 4 cut(s) 213, 249, 546, 914
MaeII ACGT 2 cut(s) 394, 748
MaeIII GTNAC 1 cut(s) 554
MalI GATC 3 cut(s) 132, 639, 1013
MbiI CCGCTC 1 cut(s) 736
MboI GATC 3 cut(s) 130, 637, 1011
MboII GAAGA 7 cut(s) 73, 85, 91, 479, 575, 761, 950
MfeI CAATTG 2 cut(s) 383, 705
MflI RGATCY 1 cut(s) 1011
MhlI GDGCHC 4 cut(s) 492, 748, 967, 1044
MluCI AATT 3 cut(s) 383, 500, 705
MlyI GAGTC 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 4 cut(s) 51, 885, 894, 989
MslI CAYNNNNRTG 2 cut(s) 287, 577
MspA1I CMGCKG 1 cut(s) 1004
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 541
MunI CAATTG 2 cut(s) 383, 705
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 1 cut(s) 541
MwoI GCNNNNNNNGC 1 cut(s) 733
NdeII GATC 3 cut(s) 130, 637, 1011
NheI GCTAGC 1 cut(s) 545
NlaIII CATG 8 cut(s) 234, 286, 340, 410, 571, 605, 686, 796
NlaIV GGNNCC 1 cut(s) 275
NspI RCATGY 1 cut(s) 796
PaeR7I CTCGAG 1 cut(s) 412
PciI ACATGT 1 cut(s) 792
PciSI GCTCTTC 1 cut(s) 774
PctI GAATGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 3 cut(s) 598, 617, 836
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 3 cut(s) 262, 738, 1003
PleI GAGTC 1 cut(s) 404
PmaCI CACGTG 1 cut(s) 749
PmlI CACGTG 1 cut(s) 749
PpsI GAGTC 1 cut(s) 404
Ppu21I YACGTR 1 cut(s) 749
PscI ACATGT 1 cut(s) 792
PshAI GACNNNNGTC 1 cut(s) 371
Psp124BI GAGCTC 3 cut(s) 492, 967, 1044
Psp1406I AACGTT 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 539
PspCI CACGTG 1 cut(s) 749
PspFI CCCAGC 1 cut(s) 1004
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 1 cut(s) 274
PspXI VCTCGAGB 1 cut(s) 412
PsuI RGATCY 1 cut(s) 1011
PvuII CAGCTG 1 cut(s) 1004
RsaI GTAC 3 cut(s) 6, 418, 1027
RsaNI GTAC 3 cut(s) 5, 417, 1026
RseI CAYNNNNRTG 2 cut(s) 287, 577
SacI GAGCTC 3 cut(s) 492, 967, 1044
SapI GCTCTTC 1 cut(s) 774
SaqAI TTAA 4 cut(s) 51, 885, 894, 989
SatI GCNGC 3 cut(s) 261, 737, 1002
Sau3AI GATC 3 cut(s) 130, 637, 1011
Sau96I GGNCC 1 cut(s) 274
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 541
SduI GDGCHC 4 cut(s) 492, 748, 967, 1044
SfaNI GCATC 6 cut(s) 127, 171, 178, 193, 306, 656
Sfr274I CTCGAG 1 cut(s) 412
SinI GGWCC 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 412
SmiMI CAYNNNNRTG 2 cut(s) 287, 577
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
Sse9I AATT 3 cut(s) 383, 500, 705
SsiI CCGC 1 cut(s) 736
SspMI CTAG 4 cut(s) 213, 249, 546, 914
SstI GAGCTC 3 cut(s) 492, 967, 1044
StyD4I CCNGG 1 cut(s) 539
StyI CCWWGG 2 cut(s) 369, 939
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 2 cut(s) 397, 751
TaqI TCGA 4 cut(s) 126, 413, 622, 961
TasI AATT 3 cut(s) 383, 500, 705
TatI WGTACW 2 cut(s) 416, 1025
TauI GCSGC 1 cut(s) 739
TfiI GAWTC 3 cut(s) 598, 617, 836
Tru1I TTAA 4 cut(s) 51, 885, 894, 989
Tru9I TTAA 4 cut(s) 51, 885, 894, 989
TscAI CASTG 2 cut(s) 271, 881
TseI GCWGC 2 cut(s) 260, 1001
TspDTI ATGAA 9 cut(s) 85, 174, 374, 387, 439, 453, 576, 590, 761
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 2 cut(s) 271, 881
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 274
XbaI TCTAGA 1 cut(s) 248
XceI RCATGY 1 cut(s) 796
XhoI CTCGAG 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 4 cut(s) 213, 249, 546, 914
ZrmI AGTACT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.