Rh2BG297800

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015813
Physical Location & Seq
Forward (+)
33998420 .. 34017092
18673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG297800.1

Sequence Viewer

Length: 1296 bp
ATGTCTATTCTCCTGCAATCAAAGCTTGAGAGTGTATCGATCAAATGGAGCCCTGTTCATCAACCCAACAGGGACTTTTCCTCTGCAGAAAGCACATCCAGAGTGAAGGAAAGTGGTCGGTCAAACATATATCGACGAGGTCTTCGTGAAGATCAGTCTGAAGAATGGTGTAGTCGCCTCCAGTCCGCTTGGGATTCTCTCTCTCAATCTCTCATCCCTCACATTACATTTTCAGATATATTTGATGAACTTCACTTCATTGAAACTCTGCAAGAAGATGTCAAGATTGTCAAGGAGCTCCCACAGGAATTGGCAAATGTTCCTCGGGCGAGGAAGCATTTCTCATCCTGGGCTAGCACCAGTTACTATGAAGACATGACACAGCTATGGAAAGAATATCAGGTGATTCATGTTGCAAAATCAGATTCTCGACTTGCAAACAATGATCTTCCTCTTGATATCCAGAGGTTGAGATGCCGTGCCTTGTATCATGCGCTCCGTTTCTCTCCCCCAATTGAGAACTTTGGAAAGAAGCTGGTGGAGCGGCTTAGAGCACGTGGGGGAAAATATATTGCTCTTCATTTGAGATATGAGAAAGACATGTTGTCTTTTACTGGTTGTTCTTATGGTTTGACTGATGAGGAATCCGAGGAACTAAGAATAATGAGGGAGAACACAAATCACTGGAAAGTTAAAAAGATTAACTCTACTGAGCAGAGACTAGGTGGCTTCTGTCCACTAACTCCCAAGGAGATTGGCATATTTCTTCGAGCTCTCGGCTATCCACCATCATCGTTAATATATATTGCAGCTGGGGAGATCTATGGTGGTAGTACTCACCTTTTGGAGCTCACATCCCGTTTTCCAAATATAGTTTTCAAGGAAACACTTGCGACCCAGGAGGAACTAAGGGCATTTACTAATCATGCATCTCAAACTGCAGCTCTTGATTATGTGATCTCAGTTGAAAGTGATGTGTTTGTCCCATCATATTCTGGTAACATGGCAAGAGCTGTTGAGGGCCAACGCAGATTCTTAGGTCACCGCAAAACGATCACTCCAGATAGGAAAGGACTTGTTGAAGTTTTTGATATGCTGGAAAATGGACAACAAACAGAAGGATCTTCGCTGTCACATATTATACAGACCATGCACAAAAATAGGAAAGGAGCTCCAAGGAGGAGAAGAGGGCCACTGCCAGAAATGAAAGGTCGAGCACGATTGAGAACTGAAGAATCTTTCTATGAAAACCCTTACCCTGAGTGTATATGTGCTTCGAAAAAGTACTTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

431

Amino Acids

49.56

Weight (kDa)

8.94

Isoelectric Point (pI)

60.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 62 - 345 5e-83 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64600
fragaria_vesca FvH4_1g24200 FvH4_1g24200 FvH4_1g24200
rosa_chinensis RchiOBHm_Chr2g0120631 RchiOBHm_Chr2g0120641
rosa_laevigata RLG00000018563
rosa_multiflora Rmu_sc0001844.1_g000005
rosa_roxburghii Rroxscaffold_2G00123270
rosa_rugosa Rorug02G0230000
rosa_samantha Rh2AG286000 Rh2BG297800 Rh2CG275500 Rh2DG311500
rosa_wichuraiana Rw2G022970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 544
AciI CCGC 3 cut(s) 186, 544, 1045
AclWI GGATC 1 cut(s) 1129
AcuI CTGAAG 2 cut(s) 180, 1251
AcvI CACGTG 1 cut(s) 557
AfaI GTAC 2 cut(s) 835, 1286
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 4 cut(s) 263, 880, 968, 1082
AhdI GACNNNNNGTC 1 cut(s) 604
AjnI CCWGG 2 cut(s) 347, 897
AjuI GAANNNNNNNTTGG 1 cut(s) 1271
Alw21I GWGCWC 6 cut(s) 300, 556, 775, 852, 1174, 1219
Alw26I GTCTC 1 cut(s) 712
AlwI GGATC 1 cut(s) 1129
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 2 cut(s) 1021, 1190
ApeKI GCWGC 2 cut(s) 809, 941
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
Asp700I GAANNNNTTC 1 cut(s) 338
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 2 cut(s) 1021, 1190
AsuHPI GGTGA 3 cut(s) 415, 830, 1034
AsuII TTCGAA 1 cut(s) 1277
AsuNHI GCTAGC 1 cut(s) 353
AvaI CYCGRG 1 cut(s) 324
BanII GRGCYC 5 cut(s) 53, 300, 775, 852, 1174
BarI GAAGNNNNNNTAC 2 cut(s) 1258, 1290
BbrPI CACGTG 1 cut(s) 557
BbsI GAAGAC 2 cut(s) 134, 378
Bbv12I GWGCWC 6 cut(s) 300, 556, 775, 852, 1174, 1219
BbvI GCAGC 2 cut(s) 821, 953
BccI CCATC 2 cut(s) 796, 994
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 2 cut(s) 349, 899
BcoDI GTCTC 1 cut(s) 712
BfaI CTAG 2 cut(s) 354, 722
BfmI CTRYAG 2 cut(s) 84, 939
BglII AGATCT 1 cut(s) 819
BisI GCNGC 3 cut(s) 545, 810, 942
BlsI GCNGC 3 cut(s) 546, 811, 943
BmcAI AGTACT 2 cut(s) 835, 1286
Bme1390I CCNGG 2 cut(s) 349, 899
BmeRI GACNNNNNGTC 1 cut(s) 604
BmeT110I CYCGRG 1 cut(s) 324
BmgT120I GGNCC 2 cut(s) 1021, 1190
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 2 cut(s) 349, 899
BmsI GCATC 2 cut(s) 464, 938
BmtI GCTAGC 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 134, 378
BpmI CTGGAG 2 cut(s) 164, 1044
Bpu14I TTCGAA 1 cut(s) 1277
BpuEI CTTGAG 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 557
BsaJI CCNNGG 6 cut(s) 323, 348, 648, 747, 897, 1175
BsaXI ACNNNNNCTCC 2 cut(s) 1042, 1072
Bse1I ACTGG 4 cut(s) 181, 360, 619, 689
BseBI CCWGG 2 cut(s) 349, 899
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 6 cut(s) 323, 348, 648, 747, 897, 1175
BseGI GGATG 4 cut(s) 95, 213, 344, 854
BseMII CTCAG 3 cut(s) 702, 975, 1251
BseNI ACTGG 4 cut(s) 181, 360, 619, 689
BseRI GAGGAG 1 cut(s) 1195
BseXI GCAGC 2 cut(s) 821, 953
BseYI CCCAGC 1 cut(s) 812
BshFI GGCC 2 cut(s) 1023, 1192
BshVI ATCGAT 1 cut(s) 38
BsiHKAI GWGCWC 6 cut(s) 300, 556, 775, 852, 1174, 1219
BsiHKCI CYCGRG 1 cut(s) 324
BslFI GGGAC 2 cut(s) 86, 968
BsmAI GTCTC 1 cut(s) 712
BsmFI GGGAC 2 cut(s) 86, 968
BsnI GGCC 2 cut(s) 1023, 1192
BsoBI CYCGRG 1 cut(s) 324
Bsp119I TTCGAA 1 cut(s) 1277
Bsp1286I GDGCHC 7 cut(s) 53, 300, 556, 775, 852, 1174, 1219
Bsp143I GATC 7 cut(s) 39, 151, 445, 819, 957, 1053, 1121
BspACI CCGC 3 cut(s) 186, 544, 1045
BspANI GGCC 2 cut(s) 1023, 1192
BspCNI CTCAG 3 cut(s) 703, 974, 1252
BspDI ATCGAT 1 cut(s) 38
BspLI GGNNCC 1 cut(s) 50
BspMAI CTGCAG 2 cut(s) 88, 943
BspOI GCTAGC 1 cut(s) 357
BspPI GGATC 1 cut(s) 1129
BspQI GCTCTTC 1 cut(s) 582
BspT104I TTCGAA 1 cut(s) 1277
BsrBI CCGCTC 1 cut(s) 544
BsrI ACTGG 4 cut(s) 181, 360, 619, 689
BssECI CCNNGG 6 cut(s) 323, 348, 648, 747, 897, 1175
BssMI GATC 7 cut(s) 39, 151, 445, 819, 957, 1053, 1121
BssT1I CCWWGG 2 cut(s) 747, 1175
Bst2UI CCWGG 2 cut(s) 349, 899
Bst6I CTCTTC 2 cut(s) 582, 1180
BstBAI YACGTR 1 cut(s) 557
BstBI TTCGAA 1 cut(s) 1277
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 7 cut(s) 548, 656, 711, 908, 961, 1036, 1260
BstEII GGTNACC 1 cut(s) 1040
BstF5I GGATG 4 cut(s) 95, 213, 344, 854
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 7 cut(s) 42, 154, 448, 822, 960, 1056, 1124
BstMAI GTCTC 1 cut(s) 712
BstMBI GATC 7 cut(s) 39, 151, 445, 819, 957, 1053, 1121
BstMWI GCNNNNNNNGC 2 cut(s) 22, 541
BstNI CCWGG 2 cut(s) 349, 899
BstNSI RCATGY 1 cut(s) 604
BstPI GGTNACC 1 cut(s) 1040
BstSCI CCNGG 2 cut(s) 347, 897
BstSFI CTRYAG 2 cut(s) 84, 939
BstV1I GCAGC 2 cut(s) 821, 953
BstV2I GAAGAC 2 cut(s) 134, 378
BstX2I RGATCY 2 cut(s) 819, 1121
BstYI RGATCY 2 cut(s) 819, 1121
Bsu15I ATCGAT 1 cut(s) 38
BsuRI GGCC 2 cut(s) 1023, 1192
BsuTUI ATCGAT 1 cut(s) 38
BtsCI GGATG 4 cut(s) 95, 213, 344, 854
BtsI GCAGTG 1 cut(s) 1193
BtsIMutI CAGTG 2 cut(s) 682, 1193
Cac8I GCNNGC 1 cut(s) 355
CfoI GCGC 1 cut(s) 496
Cfr13I GGNCC 2 cut(s) 1021, 1190
ClaI ATCGAT 1 cut(s) 38
Csp6I GTAC 2 cut(s) 834, 1285
CspCI CAANNNNNGTGG 2 cut(s) 291, 326
CviAII CATG 7 cut(s) 376, 410, 491, 601, 926, 1003, 1150
CviQI GTAC 2 cut(s) 834, 1285
DdeI CTNAG 7 cut(s) 548, 656, 711, 908, 961, 1036, 1260
DpnI GATC 7 cut(s) 41, 153, 447, 821, 959, 1055, 1123
DpnII GATC 7 cut(s) 39, 151, 445, 819, 957, 1053, 1121
DriI GACNNNNNGTC 1 cut(s) 604
Eam1104I CTCTTC 2 cut(s) 582, 1180
Eam1105I GACNNNNNGTC 1 cut(s) 604
EarI CTCTTC 2 cut(s) 582, 1180
Ecl136II GAGCTC 4 cut(s) 298, 773, 850, 1172
Eco130I CCWWGG 2 cut(s) 747, 1175
Eco24I GRGCYC 5 cut(s) 53, 300, 775, 852, 1174
Eco32I GATATC 1 cut(s) 460
Eco53kI GAGCTC 4 cut(s) 298, 773, 850, 1172
Eco57I CTGAAG 2 cut(s) 180, 1251
Eco72I CACGTG 1 cut(s) 557
Eco88I CYCGRG 1 cut(s) 324
Eco91I GGTNACC 1 cut(s) 1040
EcoICRI GAGCTC 4 cut(s) 298, 773, 850, 1172
EcoO65I GGTNACC 1 cut(s) 1040
EcoRII CCWGG 2 cut(s) 347, 897
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 2 cut(s) 747, 1175
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 5 cut(s) 53, 300, 775, 852, 1174
ErhI CCWWGG 2 cut(s) 747, 1175
FaeI CATG 7 cut(s) 379, 413, 494, 604, 929, 1006, 1153
FaqI GGGAC 2 cut(s) 86, 968
FatI CATG 7 cut(s) 375, 409, 490, 600, 925, 1002, 1149
Fnu4HI GCNGC 3 cut(s) 545, 810, 942
FokI GGATG 4 cut(s) 82, 200, 331, 841
FriOI GRGCYC 5 cut(s) 53, 300, 775, 852, 1174
Fsp4HI GCNGC 3 cut(s) 545, 810, 942
FspBI CTAG 2 cut(s) 354, 722
GlaI GCGC 1 cut(s) 495
GluI GCNGC 3 cut(s) 545, 810, 942
GsaI CCCAGC 1 cut(s) 816
GsuI CTGGAG 2 cut(s) 164, 1044
HaeIII GGCC 2 cut(s) 1023, 1192
HhaI GCGC 1 cut(s) 496
Hin1II CATG 7 cut(s) 379, 413, 494, 604, 929, 1006, 1153
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 6 cut(s) 194, 406, 425, 644, 1032, 1235
HphI GGTGA 3 cut(s) 415, 830, 1034
Hpy166II GTNNAC 1 cut(s) 737
Hpy188I TCNGA 4 cut(s) 160, 235, 424, 649
Hpy188III TCNNGA 8 cut(s) 99, 146, 283, 429, 455, 463, 947, 1061
Hpy8I GTNNAC 1 cut(s) 737
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 2 cut(s) 100, 1112
HpyCH4IV ACGT 1 cut(s) 556
HpyCH4V TGCA 9 cut(s) 16, 86, 271, 416, 437, 809, 929, 941, 1153
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 541
HpyF3I CTNAG 7 cut(s) 548, 656, 711, 908, 961, 1036, 1260
HpySE526I ACGT 1 cut(s) 556
Hsp92II CATG 7 cut(s) 379, 413, 494, 604, 929, 1006, 1153
HspAI GCGC 1 cut(s) 494
Kzo9I GATC 7 cut(s) 39, 151, 445, 819, 957, 1053, 1121
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 8 cut(s) 48, 295, 303, 501, 541, 847, 1169, 1177
Lsp1109I GCAGC 2 cut(s) 821, 953
LweI GCATC 2 cut(s) 464, 938
MaeI CTAG 2 cut(s) 354, 722
MaeII ACGT 1 cut(s) 556
MaeIII GTNAC 4 cut(s) 362, 998, 1040, 1131
MalI GATC 7 cut(s) 41, 153, 447, 821, 959, 1055, 1123
MbiI CCGCTC 1 cut(s) 544
MboI GATC 7 cut(s) 39, 151, 445, 819, 957, 1053, 1121
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 2 cut(s) 819, 1121
MhlI GDGCHC 7 cut(s) 53, 300, 556, 775, 852, 1174, 1219
MluCI AATT 2 cut(s) 308, 513
Mph1103I ATGCAT 1 cut(s) 931
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 3 cut(s) 693, 702, 797
MslI CAYNNNNRTG 1 cut(s) 385
MspA1I CMGCKG 1 cut(s) 812
MspR9I CCNGG 2 cut(s) 349, 899
MunI CAATTG 1 cut(s) 513
MvaI CCWGG 2 cut(s) 349, 899
MwoI GCNNNNNNNGC 2 cut(s) 22, 541
NdeII GATC 7 cut(s) 39, 151, 445, 819, 957, 1053, 1121
NheI GCTAGC 1 cut(s) 353
NlaIII CATG 7 cut(s) 379, 413, 494, 604, 929, 1006, 1153
NlaIV GGNNCC 1 cut(s) 50
NmeAIII GCCGAG 1 cut(s) 756
NmuCI GTSAC 2 cut(s) 1040, 1131
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 1 cut(s) 604
NspV TTCGAA 1 cut(s) 1277
PciI ACATGT 1 cut(s) 600
PciSI GCTCTTC 1 cut(s) 582
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 6 cut(s) 194, 406, 425, 644, 1032, 1235
PflFI GACNNNGTC 1 cut(s) 138
PkrI GCNGC 3 cut(s) 546, 811, 943
PmaCI CACGTG 1 cut(s) 557
PmlI CACGTG 1 cut(s) 557
Ppu21I YACGTR 1 cut(s) 557
PscI ACATGT 1 cut(s) 600
Psp124BI GAGCTC 4 cut(s) 300, 775, 852, 1174
Psp6I CCWGG 2 cut(s) 347, 897
PspCI CACGTG 1 cut(s) 557
PspEI GGTNACC 1 cut(s) 1040
PspFI CCCAGC 1 cut(s) 812
PspGI CCWGG 2 cut(s) 347, 897
PspN4I GGNNCC 1 cut(s) 50
PspPI GGNCC 2 cut(s) 1021, 1190
PstI CTGCAG 2 cut(s) 88, 943
PsuI RGATCY 2 cut(s) 819, 1121
PsyI GACNNNGTC 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 812
RsaI GTAC 2 cut(s) 835, 1286
RsaNI GTAC 2 cut(s) 834, 1285
RseI CAYNNNNRTG 1 cut(s) 385
SacI GAGCTC 4 cut(s) 300, 775, 852, 1174
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 3 cut(s) 693, 702, 797
SatI GCNGC 3 cut(s) 545, 810, 942
Sau3AI GATC 7 cut(s) 39, 151, 445, 819, 957, 1053, 1121
Sau96I GGNCC 2 cut(s) 1021, 1190
ScaI AGTACT 2 cut(s) 835, 1286
ScrFI CCNGG 2 cut(s) 349, 899
SduI GDGCHC 7 cut(s) 53, 300, 556, 775, 852, 1174, 1219
SfaNI GCATC 2 cut(s) 464, 938
SfcI CTRYAG 2 cut(s) 84, 939
SfuI TTCGAA 1 cut(s) 1277
SmiMI CAYNNNNRTG 1 cut(s) 385
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 2 cut(s) 308, 513
SsiI CCGC 3 cut(s) 186, 544, 1045
SspMI CTAG 2 cut(s) 354, 722
SstI GAGCTC 4 cut(s) 300, 775, 852, 1174
StyD4I CCNGG 2 cut(s) 347, 897
StyI CCWWGG 2 cut(s) 747, 1175
TaiI ACGT 1 cut(s) 559
TaqI TCGA 6 cut(s) 38, 133, 430, 769, 1213, 1277
TaqII GACCGA 1 cut(s) 108
TasI AATT 2 cut(s) 308, 513
TatI WGTACW 2 cut(s) 833, 1284
TauI GCSGC 1 cut(s) 547
TfiI GAWTC 6 cut(s) 194, 406, 425, 644, 1032, 1235
Tru1I TTAA 3 cut(s) 693, 702, 797
Tru9I TTAA 3 cut(s) 693, 702, 797
TscAI CASTG 2 cut(s) 689, 1200
TseFI GTSAC 2 cut(s) 1040, 1131
TseI GCWGC 2 cut(s) 809, 941
Tsp45I GTSAC 2 cut(s) 1040, 1131
TspDTI ATGAA 8 cut(s) 47, 247, 261, 384, 398, 569, 1220, 1260
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 2 cut(s) 689, 1200
Tth111I GACNNNGTC 1 cut(s) 138
XceI RCATGY 1 cut(s) 604
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 2 cut(s) 354, 722
ZrmI AGTACT 2 cut(s) 835, 1286
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.