AT5G65470

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014966
Physical Location & Seq
Forward (+)
26172009 .. 26175132
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65470.1

Sequence Viewer

Length: 1515 bp
ATGAATCCTTATGATGCGAAAGACAGAAGATGGATTCTCTCTATGTTCTTCTTCGTCGTTTTGTTCTGCAACAACGTTTCGACTTCGTCTTCTTCATCTGAAGTTATCACGATTAAGCCAAGACATTTATCCTTGCTAAAGAGTGCTTTACAACGGTCAAGTGGAGAACAGTCTGATCTATGGAGGCCATTGACTGATCAAGGATGGAGTCCTTGTATTGATTTGGGAAATTCTCCTTCATTACCGGATAAAACCGCGGGATATGTTCAGGTATTTCTTGATGGAGGACTTAACCAGCAACGAATGGGAATATGTGATGCTGTTGCAGTTGCGAAGATTTTAAACGCAACACTCGTGATCCCTTATCTTGAAGTGAATCCTGTGTGGCAAGATTCAAGTTCTTTTGTTGATATTTTTGATGTCGATCATTTTATCGATTCTTTAAAAGATGACATAAGGGTGGTTAGAGAACTACCTGATGAATACTCTTGGAGTACAAGAGAGTATTACGGTACAGCAGTACGTGAAACACGAGTGAAAACCGCGCCTGTTCATGCCTCTGCGAATTGGTATATTGAGAACGTCTCACCAGTTCTACAAAGTTATGGGATTGCTGCCATTTCTCCATTCTCTCACCGGCTATCATTCGACCACTTACCTGCTGAGATCCAACGACTAAGATGCAAAGTGAATTTCCAAGCTTTACGTTTTGTCCCTCACATTACATCACTCGGTGATGCTCTTGTCAGCCGCCTTAGAAACCCGTCATGGAGAAGCAATAAGGAACAGAAAAATGTTGATCATTTAGGAGACATGACCAACCCACACAGAAGACAAGAACCCGGGAAGTTTGCTGTGCTTCATCTTCGCTTCGACAAGGACATGGCTGCGCATTCAGCGTGTGATTTTGGCGGAGGCAAAGCCGAGAAACTCTCCTTGGCGAAATACAGACAAATGATTTGGCAAGGAAGAGTGTTGAATTCTCAATTCACTGATGAAGAGTTAAGAAGTCAAGGACGGTGTCCTCTAACACCAGAAGAAATGGGACTTCTTTTAGCTGCTTTCGGATTCGATAACAATACTCGTCTCTATCTTGCATCCCACAAGGTGTATGGAGGAGAAGCTAGAATCTCAACATTAAGACAAGTATTCCCAAGAATGGAAGACAAAAGAAGTCTTGCATCTTCTGAAGAACGAGCTCGAATCAAGGGCAAAGCTTCTTTATTAGCTGCACTTGACTACTACGTTAGTATGCACAGCGACATTTTCATCTCCGCATCACCTGGAAACATGCACAACGCTCTTGTTGGTCATCGAACATTCGAGAATCTCAAGACGATCAGACCAAACATGGCGTTAATAGGACAGCTTTTCCTCAACAAAAGCATCACTTGGGTAGATTTTCAGCAAGCGCTTGGTGAAGGACATGTGAATAGACAAGGACAGATCCGGTTAAGGAAACCAAAACAGTCGATTTATACTTATCCTGCTCCTGATTGTATGTGCCATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

504

Amino Acids

57.07

Weight (kDa)

8.96

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 88 - 441 1.7e-90 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65470
fragaria_vesca FvH4_1g19680 FvH4_1g19680
malus_domestica MD01G1039200.v1.1 MD15G1312400.v1.1
prunus_persica Prupe.6G201400_v2.0.a1 Prupe.6G201400_v2.0.a1
pyrus_communis pycom01g06690
rosa_chinensis RchiOBHm_Chr2g0111231
rosa_laevigata RLG00000017876
rosa_multiflora Rmu_sc0003055.1_g000005
rosa_roxburghii Rroxscaffold_2G00132600
rosa_rugosa Rorug02G0171900 Rorug02G0171900
rosa_samantha Rh2AG225000
rosa_wichuraiana Rw2G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 891
Acc36I ACCTGC 1 cut(s) 667
AccII CGCG 2 cut(s) 257, 545
AciI CCGC 6 cut(s) 255, 257, 543, 751, 912, 1275
AclI AACGTT 1 cut(s) 75
AclWI GGATC 3 cut(s) 352, 661, 1441
AcsI RAATTY 3 cut(s) 229, 691, 979
AcuI CTGAAG 2 cut(s) 120, 1209
AdeI CACNNNGTG 2 cut(s) 734, 1108
AfaI GTAC 3 cut(s) 496, 514, 522
AfeI AGCGCT 1 cut(s) 1413
AfiI CCNNNNNNNGG 1 cut(s) 1159
AflIII ACRYGT 1 cut(s) 1426
AgsI TTSAA 3 cut(s) 371, 396, 979
AjnI CCWGG 1 cut(s) 1282
AjuI GAANNNNNNNTTGG 2 cut(s) 920, 952
AluBI AGCT 7 cut(s) 701, 1058, 1124, 1199, 1217, 1229, 1369
AluI AGCT 7 cut(s) 701, 1058, 1124, 1199, 1217, 1229, 1369
Alw21I GWGCWC 1 cut(s) 1201
Alw26I GTCTC 3 cut(s) 589, 804, 1091
AlwI GGATC 3 cut(s) 352, 661, 1441
Ama87I CYCGRG 1 cut(s) 842
Aor51HI AGCGCT 1 cut(s) 1413
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 4 cut(s) 614, 887, 1058, 1229
ApoI RAATTY 3 cut(s) 229, 691, 979
ArsI GACNNNNNNTTYG 2 cut(s) 942, 974
AspLEI GCGC 3 cut(s) 547, 892, 1414
AsuC2I CCSGG 2 cut(s) 843, 844
AsuHPI GGTGA 5 cut(s) 579, 626, 746, 1272, 1430
AvaI CYCGRG 1 cut(s) 842
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BanII GRGCYC 1 cut(s) 1201
BauI CACGAG 2 cut(s) 353, 531
BbsI GAAGAC 3 cut(s) 81, 838, 1170
Bbv12I GWGCWC 1 cut(s) 1201
BbvI GCAGC 4 cut(s) 601, 874, 1045, 1216
BccI CCATC 3 cut(s) 24, 198, 275
BcgI CGANNNNNNTGC 2 cut(s) 663, 697
BciT130I CCWGG 1 cut(s) 1284
BclI TGATCA 2 cut(s) 196, 799
BcnI CCSGG 2 cut(s) 843, 844
BcoDI GTCTC 3 cut(s) 589, 804, 1091
BfaI CTAG 1 cut(s) 1125
BfoI RGCGCY 1 cut(s) 1415
BfuAI ACCTGC 1 cut(s) 667
BisI GCNGC 5 cut(s) 615, 751, 888, 1059, 1230
BlsI GCNGC 5 cut(s) 616, 752, 889, 1060, 1231
Bme1390I CCNGG 3 cut(s) 843, 844, 1284
BmeT110I CYCGRG 1 cut(s) 842
BmrFI CCNGG 3 cut(s) 843, 844, 1284
BmsI GCATC 8 cut(s) 4, 307, 671, 727, 1106, 1190, 1286, 1395
BpiI GAAGAC 3 cut(s) 81, 838, 1170
BplI GAGNNNNNCTC 4 cut(s) 569, 601, 917, 949
BpuEI CTTGAG 1 cut(s) 1316
BpuMI CCSGG 2 cut(s) 843, 844
Bsa29I ATCGAT 1 cut(s) 435
BsaAI YACGTR 1 cut(s) 524
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 3 cut(s) 255, 842, 936
BsaWI WCCGGW 2 cut(s) 244, 1449
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 1 cut(s) 1159
Bse118I RCCGGY 1 cut(s) 636
Bse1I ACTGG 1 cut(s) 590
Bse8I GATNNNNATC 1 cut(s) 423
BseBI CCWGG 1 cut(s) 1284
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 3 cut(s) 255, 842, 936
BseGI GGATG 2 cut(s) 209, 1097
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 1159
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 1 cut(s) 590
BseRI GAGGAG 1 cut(s) 1131
BseXI GCAGC 4 cut(s) 601, 874, 1045, 1216
BsgI GTGCAG 1 cut(s) 1215
Bsh1236I CGCG 2 cut(s) 257, 545
BshFI GGCC 1 cut(s) 187
BshVI ATCGAT 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 1201
BsiHKCI CYCGRG 1 cut(s) 842
BsiSI CCGG 4 cut(s) 245, 637, 843, 1450
BslFI GGGAC 2 cut(s) 698, 1059
BslI CCNNNNNNNGG 1 cut(s) 1159
BsmAI GTCTC 3 cut(s) 589, 804, 1091
BsmBI CGTCTC 2 cut(s) 589, 1091
BsmFI GGGAC 2 cut(s) 698, 1059
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 842
Bsp1286I GDGCHC 1 cut(s) 1201
Bsp143I GATC 8 cut(s) 175, 196, 357, 424, 666, 799, 1338, 1446
BspACI CCGC 6 cut(s) 255, 257, 543, 751, 912, 1275
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 655
BspDI ATCGAT 1 cut(s) 435
BspFNI CGCG 2 cut(s) 257, 545
BspMI ACCTGC 1 cut(s) 667
BspPI GGATC 3 cut(s) 352, 661, 1441
BsrFI RCCGGY 1 cut(s) 636
BsrI ACTGG 1 cut(s) 590
BssAI RCCGGY 1 cut(s) 636
BssECI CCNNGG 3 cut(s) 255, 842, 936
BssMI GATC 8 cut(s) 175, 196, 357, 424, 666, 799, 1338, 1446
BssSI CACGAG 2 cut(s) 353, 531
BssT1I CCWWGG 1 cut(s) 936
Bst2BI CACGAG 2 cut(s) 353, 531
Bst2UI CCWGG 1 cut(s) 1284
Bst4CI ACNGT 5 cut(s) 156, 171, 512, 1020, 1470
Bst6I CTCTTC 2 cut(s) 964, 993
BstBAI YACGTR 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 1410
BstDEI CTNAG 3 cut(s) 663, 677, 755
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 2 cut(s) 209, 1097
BstFNI CGCG 2 cut(s) 257, 545
BstH2I RGCGCY 1 cut(s) 1415
BstHHI GCGC 3 cut(s) 547, 892, 1414
BstKTI GATC 8 cut(s) 178, 199, 360, 427, 669, 802, 1341, 1449
BstMAI GTCTC 3 cut(s) 589, 804, 1091
BstMBI GATC 8 cut(s) 175, 196, 357, 424, 666, 799, 1338, 1446
BstMWI GCNNNNNNNGC 1 cut(s) 896
BstNI CCWGG 1 cut(s) 1284
BstNSI RCATGY 2 cut(s) 1294, 1430
BstSCI CCNGG 3 cut(s) 841, 842, 1282
BstUI CGCG 2 cut(s) 257, 545
BstV1I GCAGC 4 cut(s) 601, 874, 1045, 1216
BstV2I GAAGAC 3 cut(s) 81, 838, 1170
BstX2I RGATCY 2 cut(s) 666, 1446
BstYI RGATCY 2 cut(s) 666, 1446
Bsu15I ATCGAT 1 cut(s) 435
BsuRI GGCC 1 cut(s) 187
BsuTUI ATCGAT 1 cut(s) 435
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 2 cut(s) 209, 1097
BtsIMutI CAGTG 1 cut(s) 990
BveI ACCTGC 1 cut(s) 667
Cac8I GCNNGC 1 cut(s) 1410
CfoI GCGC 3 cut(s) 547, 892, 1414
Cfr10I RCCGGY 1 cut(s) 636
Cfr42I CCGCGG 1 cut(s) 258
Cfr9I CCCGGG 1 cut(s) 842
ClaI ATCGAT 1 cut(s) 435
Csp6I GTAC 3 cut(s) 495, 513, 521
CviAII CATG 8 cut(s) 554, 768, 814, 883, 1291, 1351, 1427, 1508
CviQI GTAC 3 cut(s) 495, 513, 521
DdeI CTNAG 3 cut(s) 663, 677, 755
DpnI GATC 8 cut(s) 177, 198, 359, 426, 668, 801, 1340, 1448
DpnII GATC 8 cut(s) 175, 196, 357, 424, 666, 799, 1338, 1446
DraI TTTAAA 2 cut(s) 342, 444
DraIII CACNNNGTG 2 cut(s) 734, 1108
Eam1104I CTCTTC 2 cut(s) 964, 993
EarI CTCTTC 2 cut(s) 964, 993
EciI GGCGGA 1 cut(s) 927
Ecl136II GAGCTC 1 cut(s) 1199
Eco130I CCWWGG 1 cut(s) 936
Eco24I GRGCYC 1 cut(s) 1201
Eco47III AGCGCT 1 cut(s) 1413
Eco53kI GAGCTC 1 cut(s) 1199
Eco57I CTGAAG 2 cut(s) 120, 1209
Eco88I CYCGRG 1 cut(s) 842
EcoICRI GAGCTC 1 cut(s) 1199
EcoRI GAATTC 1 cut(s) 979
EcoRII CCWGG 1 cut(s) 1282
EcoT14I CCWWGG 1 cut(s) 936
EcoT38I GRGCYC 1 cut(s) 1201
ErhI CCWWGG 1 cut(s) 936
Esp3I CGTCTC 2 cut(s) 589, 1091
FaeI CATG 8 cut(s) 557, 771, 817, 886, 1294, 1354, 1430, 1511
FalI AAGNNNNNCTT 2 cut(s) 1375, 1407
FaqI GGGAC 2 cut(s) 698, 1059
FatI CATG 8 cut(s) 553, 767, 813, 882, 1290, 1350, 1426, 1507
FauI CCCGC 1 cut(s) 250
FbaI TGATCA 2 cut(s) 196, 799
Fnu4HI GCNGC 5 cut(s) 615, 751, 888, 1059, 1230
FokI GGATG 2 cut(s) 216, 1084
FriOI GRGCYC 1 cut(s) 1201
Fsp4HI GCNGC 5 cut(s) 615, 751, 888, 1059, 1230
FspBI CTAG 1 cut(s) 1125
FspI TGCGCA 1 cut(s) 891
GlaI GCGC 3 cut(s) 546, 891, 1413
GluI GCNGC 5 cut(s) 615, 751, 888, 1059, 1230
HaeII RGCGCY 1 cut(s) 1415
HaeIII GGCC 1 cut(s) 187
HapII CCGG 4 cut(s) 245, 637, 843, 1450
HhaI GCGC 3 cut(s) 547, 892, 1414
Hin1II CATG 8 cut(s) 557, 771, 817, 886, 1294, 1354, 1430, 1511
Hin6I GCGC 3 cut(s) 545, 890, 1412
HinP1I GCGC 3 cut(s) 545, 890, 1412
HindIII AAGCTT 2 cut(s) 699, 1215
HpaII CCGG 4 cut(s) 245, 637, 843, 1450
HphI GGTGA 5 cut(s) 579, 626, 746, 1272, 1430
Hpy188I TCNGA 5 cut(s) 100, 175, 1067, 1189, 1343
Hpy188III TCNNGA 7 cut(s) 109, 278, 355, 368, 1324, 1333, 1493
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 2 cut(s) 246, 1415
HpyCH4III ACNGT 5 cut(s) 156, 171, 512, 1020, 1470
HpyCH4IV ACGT 5 cut(s) 75, 523, 582, 706, 1245
HpyCH4V TGCA 8 cut(s) 69, 326, 684, 1097, 1181, 1232, 1255, 1294
HpyF10VI GCNNNNNNNGC 1 cut(s) 896
HpyF3I CTNAG 3 cut(s) 663, 677, 755
HpySE526I ACGT 5 cut(s) 75, 523, 582, 706, 1245
Hsp92II CATG 8 cut(s) 557, 771, 817, 886, 1294, 1354, 1430, 1511
HspAI GCGC 3 cut(s) 545, 890, 1412
Ksp22I TGATCA 2 cut(s) 196, 799
KspI CCGCGG 1 cut(s) 258
Kzo9I GATC 8 cut(s) 175, 196, 357, 424, 666, 799, 1338, 1446
LmnI GCTCC 1 cut(s) 1495
Lsp1109I GCAGC 4 cut(s) 601, 874, 1045, 1216
LweI GCATC 8 cut(s) 4, 307, 671, 727, 1106, 1190, 1286, 1395
MaeI CTAG 1 cut(s) 1125
MaeII ACGT 5 cut(s) 75, 523, 582, 706, 1245
MalI GATC 8 cut(s) 177, 198, 359, 426, 668, 801, 1340, 1448
MboI GATC 8 cut(s) 175, 196, 357, 424, 666, 799, 1338, 1446
MflI RGATCY 2 cut(s) 666, 1446
MhlI GDGCHC 1 cut(s) 1201
MluCI AATT 5 cut(s) 229, 565, 691, 979, 986
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 1 cut(s) 694
MnlI CCTC 8 cut(s) 177, 278, 568, 726, 908, 1035, 1109, 1385
MseI TTAA 8 cut(s) 114, 291, 341, 443, 1004, 1139, 1358, 1454
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 4 cut(s) 245, 637, 843, 1450
MspR9I CCNGG 3 cut(s) 843, 844, 1284
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 1 cut(s) 1284
MvnI CGCG 2 cut(s) 257, 545
MwoI GCNNNNNNNGC 1 cut(s) 896
NciI CCSGG 2 cut(s) 843, 844
NdeII GATC 8 cut(s) 175, 196, 357, 424, 666, 799, 1338, 1446
NlaIII CATG 8 cut(s) 557, 771, 817, 886, 1294, 1354, 1430, 1511
NmeAIII GCCGAG 1 cut(s) 949
NsbI TGCGCA 1 cut(s) 891
NspI RCATGY 2 cut(s) 1294, 1430
PciI ACATGT 1 cut(s) 1426
PcsI WCGNNNNNNNCGW 2 cut(s) 351, 529
PctI GAATGC 1 cut(s) 892
PfeI GAWTC 9 cut(s) 4, 34, 376, 392, 437, 1068, 1128, 1203, 1327
PflFI GACNNNGTC 2 cut(s) 85, 1020
PkrI GCNGC 5 cut(s) 616, 752, 889, 1060, 1231
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
Ppu21I YACGTR 1 cut(s) 524
PscI ACATGT 1 cut(s) 1426
Psp124BI GAGCTC 1 cut(s) 1201
Psp1406I AACGTT 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 1282
PspGI CCWGG 1 cut(s) 1282
PsuI RGATCY 2 cut(s) 666, 1446
PsyI GACNNNGTC 2 cut(s) 85, 1020
RsaI GTAC 3 cut(s) 496, 514, 522
RsaNI GTAC 3 cut(s) 495, 513, 521
RseI CAYNNNNRTG 1 cut(s) 458
SacI GAGCTC 1 cut(s) 1201
SacII CCGCGG 1 cut(s) 258
SaqAI TTAA 8 cut(s) 114, 291, 341, 443, 1004, 1139, 1358, 1454
SatI GCNGC 5 cut(s) 615, 751, 888, 1059, 1230
Sau3AI GATC 8 cut(s) 175, 196, 357, 424, 666, 799, 1338, 1446
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 3 cut(s) 843, 844, 1284
SduI GDGCHC 1 cut(s) 1201
SfaNI GCATC 8 cut(s) 4, 307, 671, 727, 1106, 1190, 1286, 1395
Sfr303I CCGCGG 1 cut(s) 258
SgrBI CCGCGG 1 cut(s) 258
SmaI CCCGGG 1 cut(s) 844
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 1331
SmoI CTYRAG 1 cut(s) 1331
Sse9I AATT 5 cut(s) 229, 565, 691, 979, 986
SsiI CCGC 6 cut(s) 255, 257, 543, 751, 912, 1275
SspMI CTAG 1 cut(s) 1125
SstI GAGCTC 1 cut(s) 1201
StyD4I CCNGG 3 cut(s) 841, 842, 1282
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 5 cut(s) 156, 171, 512, 1020, 1470
TaiI ACGT 5 cut(s) 78, 526, 585, 709, 1248
TasI AATT 5 cut(s) 229, 565, 691, 979, 986
TatI WGTACW 1 cut(s) 494
TauI GCSGC 1 cut(s) 753
TfiI GAWTC 9 cut(s) 4, 34, 376, 392, 437, 1068, 1128, 1203, 1327
Tru1I TTAA 8 cut(s) 114, 291, 341, 443, 1004, 1139, 1358, 1454
Tru9I TTAA 8 cut(s) 114, 291, 341, 443, 1004, 1139, 1358, 1454
TscAI CASTG 1 cut(s) 997
TseI GCWGC 4 cut(s) 614, 887, 1058, 1229
TspDTI ATGAA 8 cut(s) 17, 84, 228, 495, 542, 851, 1011, 1258
TspMI CCCGGG 1 cut(s) 842
TspRI CASTG 1 cut(s) 997
Tth111I GACNNNGTC 2 cut(s) 85, 1020
XapI RAATTY 3 cut(s) 229, 691, 979
XceI RCATGY 2 cut(s) 1294, 1430
XcmI CCANNNNNNNNNTGG 1 cut(s) 1109
XmaI CCCGGG 1 cut(s) 842
XspI CTAG 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.