Rh2AG225000

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014966
Physical Location & Seq
Reverse (-)
23743894 .. 23749641
5748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG225000.1

Sequence Viewer

Length: 1437 bp
ATGGTGAAGTCGAATGCACAGGAAGGGAATGTTCCAAAATCTAGGCATCAACGCCTTCTTAGGAGTGCTTTACAACGCCAAACTTCAGAAGAAGAACTGTCAGAGTTGTGGACACCATTGGCCAACCAAGCCTGGAAACCTTGCATAGAACCATCAGTTGCCCCTTCATTACCAGAGAAATCTGAGGGATTCATTCAAGTATTTCTCGATGGAGGGCTGAACCAGCAGAGGATGGGGATATGTGATGCAGTCGCTGTTGCTAAAATACTGAATGCAACTCTTGTGATTCCATACCTCGAAGTGAATCCTGTTTGGCGAGATTCAAGCTCCTTCATGGATATATTTGATGTTGATCATTTCATTAATGTATTGAAGGATGATATATCCATAGTTAAAGAGCTACCTTCGGACTTCTCCTGGAGCACAAGGGAGTACTATGCAACTGCCATCCGAGCTACGAGAGTTAAGACAGCACCTGTTCATGCTTCAGCCAACTGGTATCTGGAGAATGTATTGCCTGTACTTCAGAGCTACGGAATTGCAGCAATTGCCCCTTTCTCTCATCGGCTGACATTTGATACCTTGCCTATGGACATCCAGCGGCTACGCTGTAAGGTGAATTTTCAAGCATTAAGTTTTGTCCCACATATAAGAGCACTTGGGGATGCTCTTATCAGCCGCCTTCGATATCCTTCTAGCAAAAGTGGAGCAATCGGCACTAATCACTTGCTGGAGATGCCTAATGTGAAAGGCAAACAAGGGGCGGGGAAATTTGTTGTTCTACATCTACGATTTGATAAGGATATGGCAGCTCATTCAGCTTGTGATTTTGGTGGTGGAAAAGCTGAAAAGCTGGCTCTGGCCAAATACAGGCAAGTGCTTTGGCAGGGAAGGGTTCTTAACTCCCAATTCACCGACAAAGAGTTGAGGAGTCAGGGTCGTTGTCCATTGACTCCAGAAGAGATTGGGTTGGTGCTTGCTGCACTGGGATTTGACAACAGTACTCGTCTCTATCTTGCTTCCCACAAGGTATACGGAGGGGAAGCTAGGATTGCAAATTTGCGACACTTGTTTCCACTGATGGAAGAAAAAAAGAGCCTTGCTTCTTCAGAAGAACGTGCCCAAATTAAAGGGAAGGCTTCTCTGTTGGCTGCACTTGATTACTACGTCAGCATGCACAGTGACATCTTTGTTTCTGCTTCTCCAGGAAACATGCACAATGCATTGGTAGGGCATCGAACTTATGAGAACTTAAAGACGATTAGGCCAAGCATGGGTTTATTGGGTCAACTCTTCCTTAACAAAAGCATTACTTGGTCAGATTTCCAGCAATCAGTAGTAGAAGGCCATGAAAACAGACAAGGACAGATCAGGTTCAGAAAGCCTAAGCAGTCGATATACACTTATCCTGCCCCGGACTGCATGTGCCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

478

Amino Acids

53.47

Weight (kDa)

9.14

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 66 - 415 4.3e-91 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65470
fragaria_vesca FvH4_1g19680 FvH4_1g19680
malus_domestica MD01G1039200.v1.1 MD15G1312400.v1.1
prunus_persica Prupe.6G201400_v2.0.a1 Prupe.6G201400_v2.0.a1
pyrus_communis pycom01g06690
rosa_chinensis RchiOBHm_Chr2g0111231
rosa_laevigata RLG00000017876
rosa_multiflora Rmu_sc0003055.1_g000005
rosa_roxburghii Rroxscaffold_2G00132600
rosa_rugosa Rorug02G0171900 Rorug02G0171900
rosa_samantha Rh2AG225000
rosa_wichuraiana Rw2G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1032
AciI CCGC 3 cut(s) 601, 679, 764
AcoI YGGCCR 2 cut(s) 120, 861
AcsI RAATTY 3 cut(s) 619, 770, 1057
AcuI CTGAAG 4 cut(s) 69, 471, 509, 1092
AfaI GTAC 3 cut(s) 434, 522, 1003
AfiI CCNNNNNNNGG 2 cut(s) 870, 1274
AgsI TTSAA 4 cut(s) 197, 324, 373, 626
AjnI CCWGG 3 cut(s) 131, 416, 1204
AluBI AGCT 9 cut(s) 327, 400, 455, 531, 812, 821, 845, 853, 1046
AluI AGCT 9 cut(s) 327, 400, 455, 531, 812, 821, 845, 853, 1046
Alw21I GWGCWC 2 cut(s) 425, 658
Alw26I GTCTC 1 cut(s) 1013
AlwNI CAGNNNCTG 2 cut(s) 254, 476
AoxI GGCC 4 cut(s) 120, 861, 1265, 1345
ApeKI GCWGC 4 cut(s) 542, 809, 980, 1151
ApoI RAATTY 3 cut(s) 619, 770, 1057
AseI ATTAAT 1 cut(s) 363
AsuC2I CCSGG 1 cut(s) 1415
AsuHPI GGTGA 3 cut(s) 16, 628, 904
BaeGI GKGCMC 1 cut(s) 1123
BalI TGGCCA 2 cut(s) 122, 863
Bbv12I GWGCWC 2 cut(s) 425, 658
BbvI GCAGC 4 cut(s) 554, 821, 967, 1138
BccI CCATC 5 cut(s) 160, 203, 226, 455, 1075
BciT130I CCWGG 3 cut(s) 133, 418, 1206
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 1 cut(s) 1415
BcoDI GTCTC 1 cut(s) 1013
BfaI CTAG 3 cut(s) 42, 696, 1047
BisI GCNGC 6 cut(s) 543, 602, 679, 810, 981, 1152
BlsI GCNGC 6 cut(s) 544, 603, 680, 811, 982, 1153
BmcAI AGTACT 2 cut(s) 434, 1003
Bme1390I CCNGG 4 cut(s) 133, 418, 1206, 1415
BmrFI CCNGG 4 cut(s) 133, 418, 1206, 1415
BmrI ACTGGG 1 cut(s) 995
BmsI GCATC 5 cut(s) 55, 235, 655, 726, 1243
BmuI ACTGGG 1 cut(s) 995
BpmI CTGGAG 5 cut(s) 439, 524, 752, 939, 1188
Bpu10I CCTNAGC 1 cut(s) 1386
BpuMI CCSGG 1 cut(s) 1415
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 1413
BsaXI ACNNNNNCTCC 2 cut(s) 922, 952
Bsc4I CCNNNNNNNGG 2 cut(s) 870, 1274
Bse1I ACTGG 2 cut(s) 500, 990
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 3 cut(s) 133, 418, 1206
BseDI CCNNGG 1 cut(s) 1413
BseGI GGATG 5 cut(s) 237, 382, 447, 594, 670
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 2 cut(s) 870, 1274
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 500, 990
BseRI GAGGAG 1 cut(s) 943
BseSI GKGCMC 1 cut(s) 1123
BseXI GCAGC 4 cut(s) 554, 821, 967, 1138
BsgI GTGCAG 2 cut(s) 966, 1137
BshFI GGCC 4 cut(s) 122, 863, 1267, 1347
BsiHKAI GWGCWC 2 cut(s) 425, 658
BsiSI CCGG 1 cut(s) 1415
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 2 cut(s) 870, 1274
BsmAI GTCTC 1 cut(s) 1013
BsmBI CGTCTC 1 cut(s) 1013
BsmFI GGGAC 1 cut(s) 626
BsmI GAATGC 2 cut(s) 19, 277
BsnI GGCC 4 cut(s) 122, 863, 1267, 1347
Bsp1286I GDGCHC 3 cut(s) 425, 658, 1123
Bsp143I GATC 2 cut(s) 352, 1368
BspACI CCGC 3 cut(s) 601, 679, 764
BspANI GGCC 4 cut(s) 122, 863, 1267, 1347
BspCNI CTCAG 1 cut(s) 175
BsrI ACTGG 2 cut(s) 500, 990
BssECI CCNNGG 1 cut(s) 1413
BssMI GATC 2 cut(s) 352, 1368
BssNAI GTATAC 1 cut(s) 1033
Bst1107I GTATAC 1 cut(s) 1033
Bst2UI CCWGG 3 cut(s) 133, 418, 1206
Bst4CI ACNGT 3 cut(s) 99, 1001, 1181
Bst6I CTCTTC 2 cut(s) 954, 1298
BstAPI GCANNNNNTGC 1 cut(s) 548
BstC8I GCNNGC 3 cut(s) 855, 978, 1175
BstDEI CTNAG 3 cut(s) 59, 183, 1386
BstF5I GGATG 5 cut(s) 237, 382, 447, 594, 670
BstKTI GATC 2 cut(s) 355, 1371
BstMAI GTCTC 1 cut(s) 1013
BstMBI GATC 2 cut(s) 352, 1368
BstMWI GCNNNNNNNGC 7 cut(s) 128, 223, 452, 548, 736, 818, 1052
BstNI CCWGG 3 cut(s) 133, 418, 1206
BstNSI RCATGY 3 cut(s) 1177, 1216, 1426
BstSCI CCNGG 4 cut(s) 131, 416, 1204, 1413
BstSLI GKGCMC 1 cut(s) 1123
BstV1I GCAGC 4 cut(s) 554, 821, 967, 1138
BstZ17I GTATAC 1 cut(s) 1033
BsuRI GGCC 4 cut(s) 122, 863, 1267, 1347
BtsCI GGATG 5 cut(s) 237, 382, 447, 594, 670
BtsIMutI CAGTG 3 cut(s) 983, 1076, 1186
Cac8I GCNNGC 3 cut(s) 855, 978, 1175
CaiI CAGNNNCTG 2 cut(s) 254, 476
Csp6I GTAC 3 cut(s) 433, 521, 1002
CviAII CATG 8 cut(s) 334, 482, 1174, 1213, 1273, 1349, 1423, 1434
CviQI GTAC 3 cut(s) 433, 521, 1002
DdeI CTNAG 3 cut(s) 59, 183, 1386
DpnI GATC 2 cut(s) 354, 1370
DpnII GATC 2 cut(s) 352, 1368
EaeI YGGCCR 2 cut(s) 120, 861
Eam1104I CTCTTC 2 cut(s) 954, 1298
EarI CTCTTC 2 cut(s) 954, 1298
Eco32I GATATC 1 cut(s) 689
Eco57I CTGAAG 4 cut(s) 69, 471, 509, 1092
EcoRII CCWGG 3 cut(s) 131, 416, 1204
EcoRV GATATC 1 cut(s) 689
EcoT22I ATGCAT 1 cut(s) 1225
Esp3I CGTCTC 1 cut(s) 1013
FaeI CATG 8 cut(s) 337, 485, 1177, 1216, 1276, 1352, 1426, 1437
FalI AAGNNNNNCTT 2 cut(s) 1297, 1329
FaqI GGGAC 1 cut(s) 626
FatI CATG 8 cut(s) 333, 481, 1173, 1212, 1272, 1348, 1422, 1433
FauI CCCGC 1 cut(s) 757
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 1 cut(s) 1032
Fnu4HI GCNGC 6 cut(s) 543, 602, 679, 810, 981, 1152
FokI GGATG 5 cut(s) 244, 389, 434, 581, 677
Fsp4HI GCNGC 6 cut(s) 543, 602, 679, 810, 981, 1152
FspBI CTAG 3 cut(s) 42, 696, 1047
GluI GCNGC 6 cut(s) 543, 602, 679, 810, 981, 1152
GsuI CTGGAG 5 cut(s) 439, 524, 752, 939, 1188
HaeIII GGCC 4 cut(s) 122, 863, 1267, 1347
HapII CCGG 1 cut(s) 1415
Hin1II CATG 8 cut(s) 337, 485, 1177, 1216, 1276, 1352, 1426, 1437
HincII GTYRAC 1 cut(s) 1289
HindII GTYRAC 1 cut(s) 1289
HinfI GANTC 6 cut(s) 189, 286, 304, 320, 931, 952
HpaII CCGG 1 cut(s) 1415
HphI GGTGA 3 cut(s) 16, 628, 904
Hpy166II GTNNAC 3 cut(s) 111, 1033, 1289
Hpy188I TCNGA 9 cut(s) 88, 103, 184, 409, 452, 528, 1111, 1321, 1379
Hpy188III TCNNGA 3 cut(s) 206, 503, 956
Hpy8I GTNNAC 3 cut(s) 111, 1033, 1289
HpyCH4III ACNGT 3 cut(s) 99, 1001, 1181
HpyCH4IV ACGT 2 cut(s) 1117, 1167
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 223, 452, 548, 736, 818, 1052
HpyF3I CTNAG 3 cut(s) 59, 183, 1386
HpySE526I ACGT 2 cut(s) 1117, 1167
Hsp92II CATG 8 cut(s) 337, 485, 1177, 1216, 1276, 1352, 1426, 1437
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 2 cut(s) 352, 1368
LmnI GCTCC 3 cut(s) 332, 420, 707
Lsp1109I GCAGC 4 cut(s) 554, 821, 967, 1138
LweI GCATC 5 cut(s) 55, 235, 655, 726, 1243
MaeI CTAG 3 cut(s) 42, 696, 1047
MaeII ACGT 2 cut(s) 1117, 1167
MaeIII GTNAC 1 cut(s) 1181
MalI GATC 2 cut(s) 354, 1370
MboI GATC 2 cut(s) 352, 1368
MboII GAAGA 7 cut(s) 101, 104, 971, 1097, 1098, 1124, 1285
MfeI CAATTG 1 cut(s) 546
MhlI GDGCHC 3 cut(s) 425, 658, 1123
MlsI TGGCCA 2 cut(s) 122, 863
MluCI AATT 7 cut(s) 537, 546, 619, 770, 908, 1057, 1125
MluNI TGGCCA 2 cut(s) 122, 863
MlyI GAGTC 2 cut(s) 940, 946
MnlI CCTC 6 cut(s) 178, 206, 222, 305, 921, 1031
Mox20I TGGCCA 2 cut(s) 122, 863
Mph1103I ATGCAT 1 cut(s) 1225
MscI TGGCCA 2 cut(s) 122, 863
MseI TTAA 8 cut(s) 363, 393, 465, 632, 900, 1128, 1253, 1299
Msp20I TGGCCA 2 cut(s) 122, 863
MspA1I CMGCKG 1 cut(s) 601
MspI CCGG 1 cut(s) 1415
MspR9I CCNGG 4 cut(s) 133, 418, 1206, 1415
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 2 cut(s) 19, 277
MvaI CCWGG 3 cut(s) 133, 418, 1206
MwoI GCNNNNNNNGC 7 cut(s) 128, 223, 452, 548, 736, 818, 1052
NciI CCSGG 1 cut(s) 1415
NdeII GATC 2 cut(s) 352, 1368
NlaIII CATG 8 cut(s) 337, 485, 1177, 1216, 1276, 1352, 1426, 1437
NmuCI GTSAC 1 cut(s) 1181
NsiI ATGCAT 1 cut(s) 1225
NspI RCATGY 3 cut(s) 1177, 1216, 1426
PaeI GCATGC 1 cut(s) 1177
PctI GAATGC 2 cut(s) 19, 277
PfeI GAWTC 4 cut(s) 189, 286, 304, 320
PfoI TCCNGGA 2 cut(s) 416, 1204
PkrI GCNGC 6 cut(s) 544, 603, 680, 811, 982, 1153
PleI GAGTC 2 cut(s) 939, 946
PpsI GAGTC 2 cut(s) 939, 946
PshBI ATTAAT 1 cut(s) 363
Psp6I CCWGG 3 cut(s) 131, 416, 1204
PspGI CCWGG 3 cut(s) 131, 416, 1204
PstNI CAGNNNCTG 2 cut(s) 254, 476
RsaI GTAC 3 cut(s) 434, 522, 1003
RsaNI GTAC 3 cut(s) 433, 521, 1002
SaqAI TTAA 8 cut(s) 363, 393, 465, 632, 900, 1128, 1253, 1299
SatI GCNGC 6 cut(s) 543, 602, 679, 810, 981, 1152
Sau3AI GATC 2 cut(s) 352, 1368
ScaI AGTACT 2 cut(s) 434, 1003
SchI GAGTC 2 cut(s) 940, 946
ScrFI CCNGG 4 cut(s) 133, 418, 1206, 1415
SduI GDGCHC 3 cut(s) 425, 658, 1123
SfaNI GCATC 5 cut(s) 55, 235, 655, 726, 1243
SphI GCATGC 1 cut(s) 1177
Sse9I AATT 7 cut(s) 537, 546, 619, 770, 908, 1057, 1125
SsiI CCGC 3 cut(s) 601, 679, 764
SspMI CTAG 3 cut(s) 42, 696, 1047
StyD4I CCNGG 4 cut(s) 131, 416, 1204, 1413
TaaI ACNGT 3 cut(s) 99, 1001, 1181
TaiI ACGT 2 cut(s) 1120, 1170
TaqI TCGA 6 cut(s) 11, 207, 297, 685, 1237, 1394
TasI AATT 7 cut(s) 537, 546, 619, 770, 908, 1057, 1125
TatI WGTACW 3 cut(s) 432, 520, 1001
TauI GCSGC 2 cut(s) 604, 681
TfiI GAWTC 4 cut(s) 189, 286, 304, 320
Tru1I TTAA 8 cut(s) 363, 393, 465, 632, 900, 1128, 1253, 1299
Tru9I TTAA 8 cut(s) 363, 393, 465, 632, 900, 1128, 1253, 1299
TscAI CASTG 3 cut(s) 990, 1083, 1186
TseFI GTSAC 1 cut(s) 1181
TseI GCWGC 4 cut(s) 542, 809, 980, 1151
Tsp45I GTSAC 1 cut(s) 1181
TspDTI ATGAA 6 cut(s) 156, 181, 322, 349, 470, 1365
TspGWI ACGGA 2 cut(s) 549, 1050
TspRI CASTG 3 cut(s) 990, 1083, 1186
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 3 cut(s) 619, 770, 1057
XceI RCATGY 3 cut(s) 1177, 1216, 1426
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 1032
XspI CTAG 3 cut(s) 42, 696, 1047
ZrmI AGTACT 2 cut(s) 434, 1003
Zsp2I ATGCAT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.