MD15G1312400.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014966
Physical Location & Seq
Reverse (-)
31495554 .. 31499478
3925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1312400.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGGTGTTCGAGCTTCAGTTTTCTCCCAGAGGAGGAGCACTGGGTGCTCTTCTGCTGCTTCTCCTTCCAGTTTTCTTGCCCACTTTGTTCAGTCCATTGGGCCATGCTTCTCCTTCCATGTACTCGGGGCTTAATGTGATGACGAAGTCGACTTTTCAGGAATGGAATGTTCCAAAATCTAAGCACCAACGCCTTCTCATGAGTGCTTTACAACGCCAAACTTCAGAAGGGGAACTATCGGATCTATGGGCTCCATTGGCCAGCGAATCATGGAAACCTTGTGCAGAATCATCAGTTGCCCCTTCATTACCGGAGAAATCTGAGGGATTCATCCAAGTGTTTCTTGATGGAGGGCTGAACCAGCAGAGGATGGGGATATGTGATGCAGTAGCAGTTGCTAGAATACTGAATGCAACCCTTGTGATCCCGTACCTTGAAGTAAATCCTGTATGGCGAGATTCGAGCTCTTTCATGGATATATTTGACGTTGATCATTTCATTAATGTATTGAAGGATGATATTAGAATAGTTAAAGAGCTACCTTCTGATTTCTCGTGGAGCACGAGGGAGTACTATGCAACAGCAATCCGAGCTACGAGAGTTAAGACAGCACCTGTCCATGCTTCAGCCCACTGGTATCTAGAGAATGTATTGCCCGTAGTTCAGAGTTATGGAATAGCAGCGATTGCCCCTTTCTCCCACCGCCTGACATTTGACAACTTGCCTATGGACATCCAACGGCTACGCTGTAAGGTCAACTTTCAAGCATTAGTGTTTGTCCCTCATATTAGAGCTCTTGGAGATGCTCTGATCAGCCGCCTTAGATATCCTTCTACCAAAAGTGGGGTAGTTGGTACCAACTACTTGCAGGAGATGCCAGATGTGAAAGAAAAGCAAGGGGCAGGGAAATTTGTTGTGCTACATTTACGGTTTGATAAGGATATGGCAGCCCATTCGGCCTGTGATTTTGGTGGTGGAAAAGCTGAAAAACTGGCTCTGGCCAAATATCGACAAGTGATCTGGCAGGGAAGGGTCCTTAACTCCCAGTTCACTGACGAAGAGTTGAGGAGTCAGGGGCGTTGTCCATTAACTCCTGAAGAGATTGGATTGCTGCTTGCAGCTTTGGGATTTGACAACAATACTCGCCTCTATCTTGCTTCCCATAAGGTATACGGTGGGGAAGCTAGGATTGCAACTTTGCGAAAATTGTTTCCACTCATGGAAGATAAAAAGAGCCTTGCTTCTTCAGAAGAACGGGCCCAAATCAAAGGAAAGGCTTCTCTGTTAGCAGCGGTTGATTACTACGTTAGCATGCACAGTGACATCTTCGTTTCTGCTTCTCCAGGAAACATGCACAATGCACTGGTAGGGCACCGAACTTACGAGAACTTGAAGACCATCAGGCCAAGTATGTCACTACTGGGCCAGCTCTTCCTAAACAAAACCCTTAGCTGGTCGGAATTCCAGCAGTCGGTAGTAAAAGGGCACGAAAACAGGCAAGGGCAAATCAGGATCAGAAAGCCTAAGCAGTCCATATACACCTATCCTGCTCCCGACTGCATGTGCGAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

525

Amino Acids

58.55

Weight (kDa)

8.96

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 112 - 461 7.4e-92 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65470
fragaria_vesca FvH4_1g19680 FvH4_1g19680
malus_domestica MD01G1039200.v1.1 MD15G1312400.v1.1
prunus_persica Prupe.6G201400_v2.0.a1 Prupe.6G201400_v2.0.a1
pyrus_communis pycom01g06690
rosa_chinensis RchiOBHm_Chr2g0111231
rosa_laevigata RLG00000017876
rosa_multiflora Rmu_sc0003055.1_g000005
rosa_roxburghii Rroxscaffold_2G00132600
rosa_rugosa Rorug02G0171900 Rorug02G0171900
rosa_samantha Rh2AG225000
rosa_wichuraiana Rw2G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 854
AccB1I GGYRCC 2 cut(s) 854, 1371
AccI GTMKAC 2 cut(s) 149, 1170
AciI CCGC 3 cut(s) 703, 817, 1292
AclWI GGATC 3 cut(s) 249, 418, 1520
AcoI YGGCCR 2 cut(s) 258, 999
AcsI RAATTY 2 cut(s) 908, 1460
AcuI CTGAAG 4 cut(s) 207, 609, 1116, 1230
AdeI CACNNNGTG 1 cut(s) 44
AfaI GTAC 4 cut(s) 122, 431, 572, 856
AfiI CCNNNNNNNGG 4 cut(s) 32, 843, 1452, 1471
AgsI TTSAA 4 cut(s) 437, 511, 764, 1393
AjnI CCWGG 1 cut(s) 1342
Alw21I GWGCWC 5 cut(s) 40, 49, 467, 563, 796
AlwI GGATC 3 cut(s) 249, 418, 1520
AlwNI CAGNNNCTG 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 124
AoxI GGCC 7 cut(s) 100, 258, 957, 999, 1257, 1403, 1423
ApaI GGGCCC 1 cut(s) 1261
ApeKI GCWGC 6 cut(s) 55, 680, 947, 1111, 1118, 1289
ApoI RAATTY 2 cut(s) 908, 1460
AseI ATTAAT 1 cut(s) 501
Asp700I GAANNNNTTC 1 cut(s) 1276
Asp718I GGTACC 1 cut(s) 854
AspS9I GGNCC 5 cut(s) 100, 1033, 1257, 1258, 1423
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 1 cut(s) 1033
BaeGI GKGCMC 3 cut(s) 1261, 1374, 1488
BalI TGGCCA 2 cut(s) 260, 1001
BanI GGYRCC 2 cut(s) 854, 1371
BanII GRGCYC 4 cut(s) 253, 467, 796, 1261
BauI CACGAG 2 cut(s) 553, 562
BbsI GAAGAC 1 cut(s) 1400
Bbv12I GWGCWC 5 cut(s) 40, 49, 467, 563, 796
BbvI GCAGC 6 cut(s) 42, 692, 959, 1098, 1130, 1301
BccI CCATC 3 cut(s) 341, 364, 1406
BceAI ACGGC 1 cut(s) 755
BcgI CGANNNNNNTGC 2 cut(s) 936, 970
BciT130I CCWGG 1 cut(s) 1344
BclI TGATCA 2 cut(s) 490, 810
BfaI CTAG 3 cut(s) 399, 641, 1185
BglI GCCNNNNNGGC 1 cut(s) 956
BisI GCNGC 7 cut(s) 56, 681, 817, 948, 1112, 1119, 1290
BlsI GCNGC 7 cut(s) 57, 682, 818, 949, 1113, 1120, 1291
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 1 cut(s) 1344
Bme18I GGWCC 1 cut(s) 1033
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 5 cut(s) 100, 1033, 1257, 1258, 1423
BmiI GGNNCC 5 cut(s) 252, 856, 1034, 1259, 1373
BmrFI CCNGG 1 cut(s) 1344
BmrI ACTGGG 3 cut(s) 50, 1039, 1430
BmsI GCATC 3 cut(s) 373, 793, 864
BmuI ACTGGG 3 cut(s) 50, 1039, 1430
BpiI GAAGAC 1 cut(s) 1400
BpmI CTGGAG 1 cut(s) 1326
Bpu10I CCTNAGC 2 cut(s) 1448, 1524
BsaWI WCCGGW 1 cut(s) 310
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 843, 1452, 1471
Bse1I ACTGG 7 cut(s) 45, 68, 638, 996, 1045, 1368, 1425
BseBI CCWGG 1 cut(s) 1344
BseGI GGATG 4 cut(s) 330, 375, 520, 732
BseLI CCNNNNNNNGG 4 cut(s) 32, 843, 1452, 1471
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 7 cut(s) 45, 68, 638, 996, 1045, 1368, 1425
BseRI GAGGAG 3 cut(s) 45, 48, 1081
BseSI GKGCMC 3 cut(s) 1261, 1374, 1488
BseXI GCAGC 6 cut(s) 42, 692, 959, 1098, 1130, 1301
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 7 cut(s) 102, 260, 959, 1001, 1259, 1405, 1425
BshNI GGYRCC 2 cut(s) 854, 1371
BsiHKAI GWGCWC 5 cut(s) 40, 49, 467, 563, 796
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 1 cut(s) 311
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 4 cut(s) 32, 843, 1452, 1471
BsmFI GGGAC 1 cut(s) 764
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 7 cut(s) 102, 260, 959, 1001, 1259, 1405, 1425
BsoBI CYCGRG 1 cut(s) 124
Bsp120I GGGCCC 1 cut(s) 1257
Bsp1286I GDGCHC 9 cut(s) 40, 49, 253, 467, 563, 796, 1261, 1374, 1488
Bsp143I GATC 6 cut(s) 241, 423, 490, 810, 1017, 1512
BspACI CCGC 3 cut(s) 703, 817, 1292
BspANI GGCC 7 cut(s) 102, 260, 959, 1001, 1259, 1405, 1425
BspCNI CTCAG 1 cut(s) 313
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 5 cut(s) 252, 856, 1034, 1259, 1373
BspPI GGATC 3 cut(s) 249, 418, 1520
BspQI GCTCTTC 2 cut(s) 54, 1436
BspT107I GGYRCC 2 cut(s) 854, 1371
BsrI ACTGG 7 cut(s) 45, 68, 638, 996, 1045, 1368, 1425
BssMI GATC 6 cut(s) 241, 423, 490, 810, 1017, 1512
BssNAI GTATAC 1 cut(s) 1171
BssSI CACGAG 2 cut(s) 553, 562
Bst1107I GTATAC 1 cut(s) 1171
Bst2BI CACGAG 2 cut(s) 553, 562
Bst2UI CCWGG 1 cut(s) 1344
Bst4CI ACNGT 3 cut(s) 930, 1175, 1319
Bst6I CTCTTC 4 cut(s) 54, 1053, 1092, 1436
BstAPI GCANNNNNTGC 3 cut(s) 44, 686, 874
BstC8I GCNNGC 4 cut(s) 262, 1116, 1313, 1427
BstDEI CTNAG 5 cut(s) 180, 321, 821, 1448, 1524
BstF5I GGATG 4 cut(s) 330, 375, 520, 732
BstKTI GATC 6 cut(s) 244, 426, 493, 813, 1020, 1515
BstMBI GATC 6 cut(s) 241, 423, 490, 810, 1017, 1512
BstMWI GCNNNNNNNGC 8 cut(s) 44, 257, 361, 590, 686, 874, 956, 1190
BstNI CCWGG 1 cut(s) 1344
BstNSI RCATGY 3 cut(s) 1315, 1354, 1564
BstSCI CCNGG 1 cut(s) 1342
BstSLI GKGCMC 3 cut(s) 1261, 1374, 1488
BstV1I GCAGC 6 cut(s) 42, 692, 959, 1098, 1130, 1301
BstV2I GAAGAC 1 cut(s) 1400
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BstZ17I GTATAC 1 cut(s) 1171
BsuRI GGCC 7 cut(s) 102, 260, 959, 1001, 1259, 1405, 1425
BtsCI GGATG 4 cut(s) 330, 375, 520, 732
BtsIMutI CAGTG 5 cut(s) 38, 631, 1050, 1324, 1361
Cac8I GCNNGC 4 cut(s) 262, 1116, 1313, 1427
CaiI CAGNNNCTG 1 cut(s) 614
CciI TCATGA 1 cut(s) 198
Cfr13I GGNCC 5 cut(s) 100, 1033, 1257, 1258, 1423
Csp6I GTAC 4 cut(s) 121, 430, 571, 855
CviQI GTAC 4 cut(s) 121, 430, 571, 855
DdeI CTNAG 5 cut(s) 180, 321, 821, 1448, 1524
DpnI GATC 6 cut(s) 243, 425, 492, 812, 1019, 1514
DpnII GATC 6 cut(s) 241, 423, 490, 810, 1017, 1512
DraIII CACNNNGTG 1 cut(s) 44
EaeI YGGCCR 2 cut(s) 258, 999
Eam1104I CTCTTC 4 cut(s) 54, 1053, 1092, 1436
EarI CTCTTC 4 cut(s) 54, 1053, 1092, 1436
Ecl136II GAGCTC 2 cut(s) 465, 794
Eco24I GRGCYC 4 cut(s) 253, 467, 796, 1261
Eco32I GATATC 1 cut(s) 827
Eco47I GGWCC 1 cut(s) 1033
Eco53kI GAGCTC 2 cut(s) 465, 794
Eco57I CTGAAG 4 cut(s) 207, 609, 1116, 1230
Eco88I CYCGRG 1 cut(s) 124
EcoICRI GAGCTC 2 cut(s) 465, 794
EcoO109I RGGNCCY 1 cut(s) 1033
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 1 cut(s) 1342
EcoRV GATATC 1 cut(s) 827
EcoT38I GRGCYC 4 cut(s) 253, 467, 796, 1261
FalI AAGNNNNNCTT 4 cut(s) 327, 359, 743, 775
FaqI GGGAC 1 cut(s) 764
FbaI TGATCA 2 cut(s) 490, 810
FblI GTMKAC 2 cut(s) 149, 1170
Fnu4HI GCNGC 7 cut(s) 56, 681, 817, 948, 1112, 1119, 1290
FokI GGATG 4 cut(s) 317, 382, 527, 719
FriOI GRGCYC 4 cut(s) 253, 467, 796, 1261
Fsp4HI GCNGC 7 cut(s) 56, 681, 817, 948, 1112, 1119, 1290
FspBI CTAG 3 cut(s) 399, 641, 1185
GluI GCNGC 7 cut(s) 56, 681, 817, 948, 1112, 1119, 1290
GsuI CTGGAG 1 cut(s) 1326
HaeIII GGCC 7 cut(s) 102, 260, 959, 1001, 1259, 1405, 1425
HapII CCGG 1 cut(s) 311
HincII GTYRAC 2 cut(s) 150, 757
HindII GTYRAC 2 cut(s) 150, 757
HinfI GANTC 5 cut(s) 266, 287, 327, 458, 1069
HpaII CCGG 1 cut(s) 311
Hpy166II GTNNAC 4 cut(s) 150, 757, 1050, 1171
Hpy188III TCNNGA 7 cut(s) 158, 199, 344, 641, 1094, 1510, 1553
Hpy8I GTNNAC 4 cut(s) 150, 757, 1050, 1171
HpyAV CCTTC 9 cut(s) 74, 123, 203, 221, 312, 505, 552, 840, 1023
HpyCH4III ACNGT 3 cut(s) 930, 1175, 1319
HpyCH4IV ACGT 2 cut(s) 486, 1305
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 257, 361, 590, 686, 874, 956, 1190
HpyF3I CTNAG 5 cut(s) 180, 321, 821, 1448, 1524
HpySE526I ACGT 2 cut(s) 486, 1305
KpnI GGTACC 1 cut(s) 858
Ksp22I TGATCA 2 cut(s) 490, 810
Kzo9I GATC 6 cut(s) 241, 423, 490, 810, 1017, 1512
LguI GCTCTTC 2 cut(s) 54, 1436
LmnI GCTCC 4 cut(s) 35, 256, 558, 1555
Lsp1109I GCAGC 6 cut(s) 42, 692, 959, 1098, 1130, 1301
LweI GCATC 3 cut(s) 373, 793, 864
MaeI CTAG 3 cut(s) 399, 641, 1185
MaeII ACGT 2 cut(s) 486, 1305
MaeIII GTNAC 2 cut(s) 1319, 1413
MalI GATC 6 cut(s) 243, 425, 492, 812, 1019, 1514
MboI GATC 6 cut(s) 241, 423, 490, 810, 1017, 1512
MboII GAAGA 9 cut(s) 41, 1070, 1109, 1235, 1236, 1262, 1318, 1405, 1423
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 9 cut(s) 40, 49, 253, 467, 563, 796, 1261, 1374, 1488
MlsI TGGCCA 2 cut(s) 260, 1001
MluCI AATT 3 cut(s) 908, 1205, 1460
MluNI TGGCCA 2 cut(s) 260, 1001
MlyI GAGTC 1 cut(s) 1078
MmeI TCCRAC 2 cut(s) 760, 1437
Mox20I TGGCCA 2 cut(s) 260, 1001
MroXI GAANNNNTTC 1 cut(s) 1276
MscI TGGCCA 2 cut(s) 260, 1001
MseI TTAA 6 cut(s) 132, 501, 531, 603, 1038, 1088
MslI CAYNNNNRTG 1 cut(s) 335
Msp20I TGGCCA 2 cut(s) 260, 1001
MspA1I CMGCKG 1 cut(s) 1292
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 1344
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 1 cut(s) 1344
MwoI GCNNNNNNNGC 8 cut(s) 44, 257, 361, 590, 686, 874, 956, 1190
NdeII GATC 6 cut(s) 241, 423, 490, 810, 1017, 1512
NlaIV GGNNCC 5 cut(s) 252, 856, 1034, 1259, 1373
NmuCI GTSAC 2 cut(s) 1319, 1413
NspI RCATGY 3 cut(s) 1315, 1354, 1564
PaeI GCATGC 1 cut(s) 1315
PagI TCATGA 1 cut(s) 198
PciSI GCTCTTC 2 cut(s) 54, 1436
PcsI WCGNNNNNNNCGW 1 cut(s) 560
PctI GAATGC 1 cut(s) 415
PdmI GAANNNNTTC 1 cut(s) 1276
PfeI GAWTC 4 cut(s) 266, 287, 327, 458
PflFI GACNNNGTC 1 cut(s) 145
PfoI TCCNGGA 1 cut(s) 1342
PkrI GCNGC 7 cut(s) 57, 682, 818, 949, 1113, 1120, 1291
PleI GAGTC 1 cut(s) 1077
PpsI GAGTC 1 cut(s) 1077
PpuMI RGGWCCY 1 cut(s) 1033
PshBI ATTAAT 1 cut(s) 501
Psp124BI GAGCTC 2 cut(s) 467, 796
Psp5II RGGWCCY 1 cut(s) 1033
Psp6I CCWGG 1 cut(s) 1342
PspGI CCWGG 1 cut(s) 1342
PspN4I GGNNCC 5 cut(s) 252, 856, 1034, 1259, 1373
PspOMI GGGCCC 1 cut(s) 1257
PspPI GGNCC 5 cut(s) 100, 1033, 1257, 1258, 1423
PspPPI RGGWCCY 1 cut(s) 1033
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 145
RsaI GTAC 4 cut(s) 122, 431, 572, 856
RsaNI GTAC 4 cut(s) 121, 430, 571, 855
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 2 cut(s) 467, 796
SalI GTCGAC 1 cut(s) 148
SapI GCTCTTC 2 cut(s) 54, 1436
SaqAI TTAA 6 cut(s) 132, 501, 531, 603, 1038, 1088
SatI GCNGC 7 cut(s) 56, 681, 817, 948, 1112, 1119, 1290
Sau3AI GATC 6 cut(s) 241, 423, 490, 810, 1017, 1512
Sau96I GGNCC 5 cut(s) 100, 1033, 1257, 1258, 1423
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 1 cut(s) 1078
ScrFI CCNGG 1 cut(s) 1344
SduI GDGCHC 9 cut(s) 40, 49, 253, 467, 563, 796, 1261, 1374, 1488
SfaNI GCATC 3 cut(s) 373, 793, 864
SinI GGWCC 1 cut(s) 1033
SmiMI CAYNNNNRTG 1 cut(s) 335
SphI GCATGC 1 cut(s) 1315
Sse9I AATT 3 cut(s) 908, 1205, 1460
SsiI CCGC 3 cut(s) 703, 817, 1292
SspMI CTAG 3 cut(s) 399, 641, 1185
SstI GAGCTC 2 cut(s) 467, 796
StyD4I CCNGG 1 cut(s) 1342
TaaI ACNGT 3 cut(s) 930, 1175, 1319
TaiI ACGT 2 cut(s) 489, 1308
TaqI TCGA 4 cut(s) 9, 149, 461, 1009
TasI AATT 3 cut(s) 908, 1205, 1460
TatI WGTACW 2 cut(s) 120, 570
TauI GCSGC 1 cut(s) 819
TfiI GAWTC 4 cut(s) 266, 287, 327, 458
Tru1I TTAA 6 cut(s) 132, 501, 531, 603, 1038, 1088
Tru9I TTAA 6 cut(s) 132, 501, 531, 603, 1038, 1088
TscAI CASTG 5 cut(s) 45, 638, 1057, 1324, 1368
TseFI GTSAC 2 cut(s) 1319, 1413
TseI GCWGC 6 cut(s) 55, 680, 947, 1111, 1118, 1289
Tsp45I GTSAC 2 cut(s) 1319, 1413
TspDTI ATGAA 4 cut(s) 294, 319, 460, 487
TspRI CASTG 5 cut(s) 45, 638, 1057, 1324, 1368
Tth111I GACNNNGTC 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 1033
VspI ATTAAT 1 cut(s) 501
XapI RAATTY 2 cut(s) 908, 1460
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 3 cut(s) 1315, 1354, 1564
XmiI GTMKAC 2 cut(s) 149, 1170
XmnI GAANNNNTTC 1 cut(s) 1276
XspI CTAG 3 cut(s) 399, 641, 1185
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.