MD01G1039200.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014966
Physical Location & Seq
Forward (+)
13495036 .. 13498782
3747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1039200.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGGTGGTCCAGTTTCAGTTTTCTCCCAGAGGAGGAGCTCTCGGTGCTCTTCTGCTCCTTCTCCTCCCAGTTTTCTTGCCCACTTTGTTCAGTCCGTTGGGCCATGCTTCTCCTTCCATGTACTCGGGGCTTGATGTGATGACGAAGCCGACTTTTCAGGAATGGAATGTTCCAAAATCTAAGCACCTACGCCTTCTCAAGAGTGCTTTACAACGCCAAACCTCAGAAGGAGAACTCTCGGATCTGTGGACCCCATTGGCTAACGAATCATGGAAGCCTTGTGCAGAATCATCAATTGCCCCTTCATTACCGGAAAAATCTGAGGGATTTATCCAAGTTTTTCTTGATGGAGGGCTGAACCAGCAGAGGATGGGGATATGTGATGCAGTAGCAGTTGCTAAAATACTGAATGCAACCCTTGTGATCCCGTACCTTGAAGTGAATCCTGTATGGCGAGATTCAAGCTCCTTCATGGATATATTTGATGTTGACCATTTCATTAATGTATTGAAGGATGATATTAGAATAGTTAAAGAGCTCCCTTCTGATTTCTCGTGGAGCACAAGGGAGTACTATGCAACGGCCATCCGAGCTACGAGAGTTAAGACAGCACCTGTCCATGCTTCAGCAAACTGGTATCTAGAGAATGTATTGCCCCTAGTTCAGAGTTATGGAATAGCAGCAATTGCCCCATTCTCACACCGCCTGACATTTGACAACTTGCCTATGGATATCCAACGACTACGCTGTAAGGTCAACTTTCAAGCATTAGTGTTTGTCCCGCATATTAGAGCTCTTGGAGATGCTCTGGTCAGCCGTCTTAGATATCCTTCTAGCAAAAGTGGGGGAGCTAGCACCAATTACTTGCAGGAGATGCCTGATGTGAATGACAAGCAAGGGGCAGGGAAATTTGTTGTGCTGCATCTACGGTTTGATAAGGATATGGCAGCCCATTCGGCCTGTGATTTTGGTGGTGGAAAAGCTGAAAAATTGGCTCTGGCCAAATACCGACAAGTGATCTGGCAGGGAAGAGTCCTTAACTCCCAGTTCACGGACGAGGAGCTGAGGAGTCAGGGGCGTTGTCCATTAACTCCAGAAGAGATTGGATTGCTTCTTGCGGCTTTAGGATTTGACAACAATACTCGGCTCTATCTTGCTTCCCACAAGGTATACGGTGGGGAAGCTAGGATTGCTACTTTGCGAAAAGTGTTTCCACTTATGGAAGATAAAAAGAGCCTTGCTTCTTCAGAAGAACGGGCCCAAATCAAAGGAAAGGCTTCTCTGTTAGCAGCAGTTGATTACTACGTTAGCATGCACAGTGATATCTTCGTATCTGCTTCCCCGGGAAATATGCACAATGCACTGGTAGGGCACCGAACTTACGAGAACTTGAAGACCATCAGGCCGAGTATGCCACTATTGGGGCAACTCTTCCTAAACAAAACTCTTAGCTGGTCGGAATTCCAGCAGTCGGTAGTAAAAGGGCACGAAAACAGGCAGGGACAAATCAGGATTAGAAAGCCTAAGCAGTCCATATACACGTATCCTGCTCCCGACTGCATGTGCCAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

525

Amino Acids

58.48

Weight (kDa)

9.03

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 112 - 461 1.3e-91 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65470
fragaria_vesca FvH4_1g19680 FvH4_1g19680
malus_domestica MD01G1039200.v1.1 MD15G1312400.v1.1
prunus_persica Prupe.6G201400_v2.0.a1 Prupe.6G201400_v2.0.a1
pyrus_communis pycom01g06690
rosa_chinensis RchiOBHm_Chr2g0111231
rosa_laevigata RLG00000017876
rosa_multiflora Rmu_sc0003055.1_g000005
rosa_roxburghii Rroxscaffold_2G00132600
rosa_rugosa Rorug02G0171900 Rorug02G0171900
rosa_samantha Rh2AG225000
rosa_wichuraiana Rw2G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1371
AccI GTMKAC 1 cut(s) 1170
AciI CCGC 3 cut(s) 703, 782, 1118
AclWI GGATC 2 cut(s) 249, 418
AcoI YGGCCR 2 cut(s) 582, 999
AcsI RAATTY 2 cut(s) 908, 1460
AcuI CTGAAG 2 cut(s) 609, 1230
AfaI GTAC 3 cut(s) 122, 431, 572
AfiI CCNNNNNNNGG 4 cut(s) 32, 1051, 1421, 1471
AflIII ACRYGT 1 cut(s) 1539
AgsI TTSAA 5 cut(s) 437, 462, 511, 764, 1393
AjnI CCWGG 1 cut(s) 1566
Alw21I GWGCWC 5 cut(s) 40, 49, 540, 563, 796
AlwI GGATC 2 cut(s) 249, 418
AlwNI CAGNNNCTG 1 cut(s) 614
Ama87I CYCGRG 2 cut(s) 124, 1342
AoxI GGCC 6 cut(s) 100, 582, 957, 999, 1257, 1403
ApaI GGGCCC 1 cut(s) 1261
ApeKI GCWGC 4 cut(s) 680, 919, 947, 1289
ApoI RAATTY 2 cut(s) 908, 1460
AseI ATTAAT 1 cut(s) 501
Asp700I GAANNNNTTC 1 cut(s) 1276
AspS9I GGNCC 5 cut(s) 7, 100, 249, 1257, 1258
AsuC2I CCSGG 2 cut(s) 1343, 1344
AsuNHI GCTAGC 1 cut(s) 851
AvaI CYCGRG 2 cut(s) 124, 1342
AvaII GGWCC 2 cut(s) 7, 249
BaeGI GKGCMC 3 cut(s) 1261, 1374, 1488
BalI TGGCCA 1 cut(s) 1001
BanI GGYRCC 1 cut(s) 1371
BanII GRGCYC 4 cut(s) 40, 540, 796, 1261
BauI CACGAG 1 cut(s) 553
BbsI GAAGAC 1 cut(s) 1400
Bbv12I GWGCWC 5 cut(s) 40, 49, 540, 563, 796
BbvCI CCTCAGC 1 cut(s) 1064
BbvI GCAGC 4 cut(s) 692, 906, 959, 1301
BccI CCATC 4 cut(s) 341, 364, 593, 1406
BceAI ACGGC 2 cut(s) 597, 801
BcgI CGANNNNNNTGC 2 cut(s) 936, 970
BciT130I CCWGG 1 cut(s) 1568
BciVI GTATCC 1 cut(s) 1554
BcnI CCSGG 2 cut(s) 1343, 1344
BfaI CTAG 5 cut(s) 641, 659, 834, 852, 1185
BfuI GTATCC 1 cut(s) 1554
BglI GCCNNNNNGGC 1 cut(s) 956
BisI GCNGC 5 cut(s) 681, 920, 948, 1119, 1290
BlsI GCNGC 5 cut(s) 682, 921, 949, 1120, 1291
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 3 cut(s) 1343, 1344, 1568
Bme18I GGWCC 2 cut(s) 7, 249
BmeT110I CYCGRG 2 cut(s) 124, 1342
BmgT120I GGNCC 5 cut(s) 7, 100, 249, 1257, 1258
BmiI GGNNCC 3 cut(s) 251, 1259, 1373
BmrFI CCNGG 3 cut(s) 1343, 1344, 1568
BmrI ACTGGG 2 cut(s) 62, 1039
BmsI GCATC 4 cut(s) 373, 793, 864, 931
BmtI GCTAGC 1 cut(s) 855
BmuI ACTGGG 2 cut(s) 62, 1039
BpiI GAAGAC 1 cut(s) 1400
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BpmI CTGGAG 1 cut(s) 1077
Bpu10I CCTNAGC 2 cut(s) 1064, 1524
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 2 cut(s) 1343, 1344
BsaAI YACGTR 1 cut(s) 1542
BsaJI CCNNGG 2 cut(s) 1341, 1342
BsaWI WCCGGW 1 cut(s) 310
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 1051, 1421, 1471
Bse1I ACTGG 5 cut(s) 10, 68, 638, 1045, 1368
BseBI CCWGG 1 cut(s) 1568
BseDI CCNNGG 2 cut(s) 1341, 1342
BseGI GGATG 3 cut(s) 375, 520, 585
BseLI CCNNNNNNNGG 4 cut(s) 32, 1051, 1421, 1471
BseMII CTCAG 3 cut(s) 237, 312, 1055
BseNI ACTGG 5 cut(s) 10, 68, 638, 1045, 1368
BseRI GAGGAG 5 cut(s) 45, 48, 53, 1073, 1081
BseSI GKGCMC 3 cut(s) 1261, 1374, 1488
BseXI GCAGC 4 cut(s) 692, 906, 959, 1301
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 6 cut(s) 102, 584, 959, 1001, 1259, 1405
BshNI GGYRCC 1 cut(s) 1371
BsiHKAI GWGCWC 5 cut(s) 40, 49, 540, 563, 796
BsiHKCI CYCGRG 2 cut(s) 124, 1342
BsiSI CCGG 2 cut(s) 311, 1343
BslFI GGGAC 2 cut(s) 764, 1515
BslI CCNNNNNNNGG 4 cut(s) 32, 1051, 1421, 1471
BsmFI GGGAC 2 cut(s) 764, 1515
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 6 cut(s) 102, 584, 959, 1001, 1259, 1405
BsoBI CYCGRG 2 cut(s) 124, 1342
Bsp120I GGGCCC 1 cut(s) 1257
Bsp1286I GDGCHC 8 cut(s) 40, 49, 540, 563, 796, 1261, 1374, 1488
Bsp143I GATC 3 cut(s) 241, 423, 1017
BspACI CCGC 3 cut(s) 703, 782, 1118
BspANI GGCC 6 cut(s) 102, 584, 959, 1001, 1259, 1405
BspCNI CTCAG 3 cut(s) 236, 313, 1056
BspLI GGNNCC 3 cut(s) 251, 1259, 1373
BspOI GCTAGC 1 cut(s) 855
BspPI GGATC 2 cut(s) 249, 418
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 1371
BsrI ACTGG 5 cut(s) 10, 68, 638, 1045, 1368
BssECI CCNNGG 2 cut(s) 1341, 1342
BssMI GATC 3 cut(s) 241, 423, 1017
BssNAI GTATAC 1 cut(s) 1171
BssSI CACGAG 1 cut(s) 553
Bst1107I GTATAC 1 cut(s) 1171
Bst2BI CACGAG 1 cut(s) 553
Bst2UI CCWGG 1 cut(s) 1568
Bst4CI ACNGT 3 cut(s) 930, 1175, 1319
Bst6I CTCTTC 4 cut(s) 54, 1024, 1092, 1436
BstAPI GCANNNNNTGC 2 cut(s) 686, 874
BstBAI YACGTR 1 cut(s) 1542
BstC8I GCNNGC 2 cut(s) 853, 1313
BstDEI CTNAG 7 cut(s) 180, 223, 321, 821, 1064, 1448, 1524
BstF5I GGATG 3 cut(s) 375, 520, 585
BstKTI GATC 3 cut(s) 244, 426, 1020
BstMBI GATC 3 cut(s) 241, 423, 1017
BstMWI GCNNNNNNNGC 8 cut(s) 44, 361, 590, 686, 874, 956, 1190, 1411
BstNI CCWGG 1 cut(s) 1568
BstNSI RCATGY 2 cut(s) 1315, 1564
BstSCI CCNGG 3 cut(s) 1341, 1342, 1566
BstSLI GKGCMC 3 cut(s) 1261, 1374, 1488
BstV1I GCAGC 4 cut(s) 692, 906, 959, 1301
BstV2I GAAGAC 1 cut(s) 1400
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BstZ17I GTATAC 1 cut(s) 1171
BsuI GTATCC 1 cut(s) 1554
BsuRI GGCC 6 cut(s) 102, 584, 959, 1001, 1259, 1405
BtsCI GGATG 3 cut(s) 375, 520, 585
BtsIMutI CAGTG 2 cut(s) 1324, 1361
Cac8I GCNNGC 2 cut(s) 853, 1313
CaiI CAGNNNCTG 1 cut(s) 614
Cfr13I GGNCC 5 cut(s) 7, 100, 249, 1257, 1258
Cfr9I CCCGGG 1 cut(s) 1342
Csp6I GTAC 3 cut(s) 121, 430, 571
CviAII CATG 8 cut(s) 104, 118, 270, 472, 620, 1312, 1561, 1572
CviQI GTAC 3 cut(s) 121, 430, 571
DdeI CTNAG 7 cut(s) 180, 223, 321, 821, 1064, 1448, 1524
DpnI GATC 3 cut(s) 243, 425, 1019
DpnII GATC 3 cut(s) 241, 423, 1017
EaeI YGGCCR 2 cut(s) 582, 999
Eam1104I CTCTTC 4 cut(s) 54, 1024, 1092, 1436
EarI CTCTTC 4 cut(s) 54, 1024, 1092, 1436
Ecl136II GAGCTC 3 cut(s) 38, 538, 794
Eco24I GRGCYC 4 cut(s) 40, 540, 796, 1261
Eco32I GATATC 3 cut(s) 733, 827, 1324
Eco47I GGWCC 2 cut(s) 7, 249
Eco53kI GAGCTC 3 cut(s) 38, 538, 794
Eco57I CTGAAG 2 cut(s) 609, 1230
Eco88I CYCGRG 2 cut(s) 124, 1342
EcoICRI GAGCTC 3 cut(s) 38, 538, 794
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 1 cut(s) 1566
EcoRV GATATC 3 cut(s) 733, 827, 1324
EcoT38I GRGCYC 4 cut(s) 40, 540, 796, 1261
FaeI CATG 8 cut(s) 107, 121, 273, 475, 623, 1315, 1564, 1575
FalI AAGNNNNNCTT 4 cut(s) 327, 359, 743, 775
FaqI GGGAC 2 cut(s) 764, 1515
FatI CATG 8 cut(s) 103, 117, 269, 471, 619, 1311, 1560, 1571
FauI CCCGC 1 cut(s) 789
FblI GTMKAC 1 cut(s) 1170
Fnu4HI GCNGC 5 cut(s) 681, 920, 948, 1119, 1290
FokI GGATG 3 cut(s) 382, 527, 572
FriOI GRGCYC 4 cut(s) 40, 540, 796, 1261
Fsp4HI GCNGC 5 cut(s) 681, 920, 948, 1119, 1290
FspBI CTAG 5 cut(s) 641, 659, 834, 852, 1185
GluI GCNGC 5 cut(s) 681, 920, 948, 1119, 1290
GsuI CTGGAG 1 cut(s) 1077
HaeIII GGCC 6 cut(s) 102, 584, 959, 1001, 1259, 1405
HapII CCGG 2 cut(s) 311, 1343
Hin1II CATG 8 cut(s) 107, 121, 273, 475, 623, 1315, 1564, 1575
HincII GTYRAC 2 cut(s) 490, 757
HindII GTYRAC 2 cut(s) 490, 757
HinfI GANTC 6 cut(s) 266, 287, 442, 458, 1032, 1069
HpaII CCGG 2 cut(s) 311, 1343
Hpy166II GTNNAC 5 cut(s) 249, 490, 757, 1050, 1171
Hpy188I TCNGA 8 cut(s) 226, 241, 322, 547, 590, 666, 1249, 1459
Hpy188III TCNNGA 7 cut(s) 158, 199, 344, 641, 1094, 1510, 1553
Hpy8I GTNNAC 5 cut(s) 249, 490, 757, 1050, 1171
HpyAV CCTTC 9 cut(s) 68, 123, 203, 221, 312, 478, 505, 552, 840
HpyCH4III ACNGT 3 cut(s) 930, 1175, 1319
HpyCH4IV ACGT 2 cut(s) 1305, 1541
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 361, 590, 686, 874, 956, 1190, 1411
HpyF3I CTNAG 7 cut(s) 180, 223, 321, 821, 1064, 1448, 1524
HpySE526I ACGT 2 cut(s) 1305, 1541
Hsp92II CATG 8 cut(s) 107, 121, 273, 475, 623, 1315, 1564, 1575
Kzo9I GATC 3 cut(s) 241, 423, 1017
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 8 cut(s) 35, 60, 470, 543, 558, 848, 1060, 1555
Lsp1109I GCAGC 4 cut(s) 692, 906, 959, 1301
LweI GCATC 4 cut(s) 373, 793, 864, 931
MaeI CTAG 5 cut(s) 641, 659, 834, 852, 1185
MaeII ACGT 2 cut(s) 1305, 1541
MalI GATC 3 cut(s) 243, 425, 1019
MboI GATC 3 cut(s) 241, 423, 1017
MboII GAAGA 9 cut(s) 41, 1041, 1109, 1235, 1236, 1262, 1318, 1405, 1423
MfeI CAATTG 2 cut(s) 294, 684
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 8 cut(s) 40, 49, 540, 563, 796, 1261, 1374, 1488
MlsI TGGCCA 1 cut(s) 1001
MluCI AATT 6 cut(s) 294, 684, 859, 908, 989, 1460
MluNI TGGCCA 1 cut(s) 1001
MlyI GAGTC 2 cut(s) 1041, 1078
MmeI TCCRAC 2 cut(s) 760, 1437
MnlI CCTC 9 cut(s) 23, 26, 74, 232, 316, 344, 360, 1051, 1059
Mox20I TGGCCA 1 cut(s) 1001
MroXI GAANNNNTTC 1 cut(s) 1276
MscI TGGCCA 1 cut(s) 1001
MseI TTAA 5 cut(s) 501, 531, 603, 1038, 1088
Msp20I TGGCCA 1 cut(s) 1001
MspI CCGG 2 cut(s) 311, 1343
MspR9I CCNGG 3 cut(s) 1343, 1344, 1568
MunI CAATTG 2 cut(s) 294, 684
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 1 cut(s) 1568
MwoI GCNNNNNNNGC 8 cut(s) 44, 361, 590, 686, 874, 956, 1190, 1411
NciI CCSGG 2 cut(s) 1343, 1344
NdeII GATC 3 cut(s) 241, 423, 1017
NheI GCTAGC 1 cut(s) 851
NlaIII CATG 8 cut(s) 107, 121, 273, 475, 623, 1315, 1564, 1575
NlaIV GGNNCC 3 cut(s) 251, 1259, 1373
NmeAIII GCCGAG 2 cut(s) 1123, 1431
NspI RCATGY 2 cut(s) 1315, 1564
PaeI GCATGC 1 cut(s) 1315
PciSI GCTCTTC 1 cut(s) 54
PctI GAATGC 1 cut(s) 415
PdmI GAANNNNTTC 1 cut(s) 1276
PfeI GAWTC 4 cut(s) 266, 287, 442, 458
PkrI GCNGC 5 cut(s) 682, 921, 949, 1120, 1291
PleI GAGTC 2 cut(s) 1040, 1077
PpsI GAGTC 2 cut(s) 1040, 1077
Ppu21I YACGTR 1 cut(s) 1542
PshBI ATTAAT 1 cut(s) 501
Psp124BI GAGCTC 3 cut(s) 40, 540, 796
Psp6I CCWGG 1 cut(s) 1566
PspGI CCWGG 1 cut(s) 1566
PspN4I GGNNCC 3 cut(s) 251, 1259, 1373
PspOMI GGGCCC 1 cut(s) 1257
PspPI GGNCC 5 cut(s) 7, 100, 249, 1257, 1258
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 3 cut(s) 122, 431, 572
RsaNI GTAC 3 cut(s) 121, 430, 571
SacI GAGCTC 3 cut(s) 40, 540, 796
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 5 cut(s) 501, 531, 603, 1038, 1088
SatI GCNGC 5 cut(s) 681, 920, 948, 1119, 1290
Sau3AI GATC 3 cut(s) 241, 423, 1017
Sau96I GGNCC 5 cut(s) 7, 100, 249, 1257, 1258
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 2 cut(s) 1041, 1078
ScrFI CCNGG 3 cut(s) 1343, 1344, 1568
SduI GDGCHC 8 cut(s) 40, 49, 540, 563, 796, 1261, 1374, 1488
SfaNI GCATC 4 cut(s) 373, 793, 864, 931
SinI GGWCC 2 cut(s) 7, 249
SmaI CCCGGG 1 cut(s) 1344
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SphI GCATGC 1 cut(s) 1315
Sse9I AATT 6 cut(s) 294, 684, 859, 908, 989, 1460
SsiI CCGC 3 cut(s) 703, 782, 1118
SspMI CTAG 5 cut(s) 641, 659, 834, 852, 1185
SstI GAGCTC 3 cut(s) 40, 540, 796
StyD4I CCNGG 3 cut(s) 1341, 1342, 1566
TaaI ACNGT 3 cut(s) 930, 1175, 1319
TaiI ACGT 2 cut(s) 1308, 1544
TasI AATT 6 cut(s) 294, 684, 859, 908, 989, 1460
TatI WGTACW 2 cut(s) 120, 570
TauI GCSGC 1 cut(s) 1121
TfiI GAWTC 4 cut(s) 266, 287, 442, 458
Tru1I TTAA 5 cut(s) 501, 531, 603, 1038, 1088
Tru9I TTAA 5 cut(s) 501, 531, 603, 1038, 1088
TscAI CASTG 2 cut(s) 1324, 1368
TseI GCWGC 4 cut(s) 680, 919, 947, 1289
TspDTI ATGAA 3 cut(s) 294, 460, 487
TspGWI ACGGA 2 cut(s) 84, 1067
TspMI CCCGGG 1 cut(s) 1342
TspRI CASTG 2 cut(s) 1324, 1368
VpaK11BI GGWCC 2 cut(s) 7, 249
VspI ATTAAT 1 cut(s) 501
XapI RAATTY 2 cut(s) 908, 1460
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 2 cut(s) 1315, 1564
XmaI CCCGGG 1 cut(s) 1342
XmiI GTMKAC 1 cut(s) 1170
XmnI GAANNNNTTC 1 cut(s) 1276
XspI CTAG 5 cut(s) 641, 659, 834, 852, 1185
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.