Rroxscaffold_2G00132600

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014966
Physical Location & Seq
Forward (+)
69617018 .. 69622056
5039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132600.1

Sequence Viewer

Length: 1590 bp
ATGGGGTTGAAGCTTCAGTACCAGAATGAGTACCAGTCTCCTAGAGGAGGAGCACTGGGTGCTCTTTTGCTGCTGCTCTTTCCCATTTTCTTGCCCAGTTTGTTCAGCCCTTTTGGTCATGCTTCACCTTCAATGCTCTCGGAATGGAATGTGATGGTGAAGTCGAATGCACAGGAAGGGAATGTTCCAAAATCTAGGCACCAACGCCTTCTTAGGAGTGCTTTACAACGCCAAACTTCAGAAGAAGAACTGTCAGAGTTGTGGACACCATTGGCCAACCAAGCCTGGAGACCTTGCATAGAACCATCAATTGCCCCTTCATTACCAGAGAAATCTGAGGGATTCATTCAAGTATTTCTCGATGGAGGGCTGAACCAGCAGAGGATGGGGATATGTGATGCAGTCGCTGTTGCTAAAATACTGAATGCAACTCTTGTGATTCCATACCTTGAAGTGAATCCTGTTTGGCGAGATTCAAGCTCCTTCATGGATATATTTGATGTTGATCATTTCATTAATGTATTGAAGGATGATATATCCATAGTTAAAGAGCTACCTTCGGACTTCTCCTGGAGCACAAGGGAGTACTATGCAACAGCCATCCGAGCTACGAGAGTTAAGACAGCACCTGTTCATGCTTCAGCCAACTGGTATCTGGAGAATGTATTGCCTATACTTCAGAGCTACGGAATTGCAGCAATTGCCCCTTTCTCTCATCGGCTGACATTTGATACCTTGCCTATGGACATCCAGCGGCTACGCTGTAAGGTGAATTTTCAAGCATTAAGTTTTGTCCCACATATAAGAGCACTTGGGGATGCTCTTATCAGCCGCCTTCGATATCCTTCTAGCAAAAGTGGAGCAATCGGCACCAATCACTTGCTGGAGATGCCTAATGTGAAAGGCAAACAAGGGGCGGGGAAATTTGTTGTTCTACATCTACGATTTGACAAGGATATGGCAGCTCATTCAGCTTGTGATTTTGGTGGTGGAAAAGCTGAAAAGCTGGCTCTGGCCAAATACAGGCAAGTGCTTTGGCAGGGAAGGGTTCTTAACTCCCAATTCACCGACAAAGAGTTGAGGAGTCAGGGTCGTTGTCCATTGACTCCAGAAGAGATTGGGTTGGTGCTTGCTGCACTGGGATTTGACAACAGTACTCGTCTCTATCTTGCTTCCCACAAGGTGTACGGAGGGGAAGCCAGGATTGCAAATTTGCGACACTTGTTTCCACTGATGGAAGAGAAAAAGAGCCTTGCTTCTTCAGAAGAACGTGCCCAAATTAAAGGGAAGGCTTCTCTGTTAGCTGCACTTGATTACTACGTCAGCATGCACAGTGACATCTTTGTTTCTGCTTCTCCGGGAAACATGCACAATGCATTGGTAGGGCATCGAACTTACGAGAACTTGAAGACGATTAGGCCAAGCATGGGTTTATTGGGTCAACTCTTCCTTAACAAAAGCATTACTTGGTCTGATTTTCAGCAATCAGTAGTAGAAGGCCATGAAAACAGACAAGGACAGATCAGGTTCAGAAAGCCTAAGCAGTCGATATACACTTATCCTGCCCCGGACTGCATGTGCCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

529

Amino Acids

59.15

Weight (kDa)

9.12

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 117 - 466 5.6e-91 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65470
fragaria_vesca FvH4_1g19680 FvH4_1g19680
malus_domestica MD01G1039200.v1.1 MD15G1312400.v1.1
prunus_persica Prupe.6G201400_v2.0.a1 Prupe.6G201400_v2.0.a1
pyrus_communis pycom01g06690
rosa_chinensis RchiOBHm_Chr2g0111231
rosa_laevigata RLG00000017876
rosa_multiflora Rmu_sc0003055.1_g000005
rosa_roxburghii Rroxscaffold_2G00132600
rosa_rugosa Rorug02G0171900 Rorug02G0171900
rosa_samantha Rh2AG225000
rosa_wichuraiana Rw2G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 198, 869
AciI CCGC 3 cut(s) 754, 832, 917
AcoI YGGCCR 2 cut(s) 273, 1014
AcsI RAATTY 3 cut(s) 772, 923, 1210
AcuI CTGAAG 4 cut(s) 222, 624, 662, 1245
AdeI CACNNNGTG 2 cut(s) 59, 1183
AfaI GTAC 5 cut(s) 20, 32, 587, 1156, 1187
AfiI CCNNNNNNNGG 3 cut(s) 47, 1023, 1427
AgsI TTSAA 8 cut(s) 10, 132, 350, 452, 477, 526, 779, 1408
AjnI CCWGG 3 cut(s) 284, 569, 1199
Alw21I GWGCWC 4 cut(s) 55, 64, 578, 811
Alw26I GTCTC 3 cut(s) 42, 283, 1166
AlwNI CAGNNNCTG 2 cut(s) 407, 629
AoxI GGCC 4 cut(s) 273, 1014, 1418, 1498
ApeKI GCWGC 6 cut(s) 70, 73, 695, 962, 1133, 1304
ApoI RAATTY 3 cut(s) 772, 923, 1210
AseI ATTAAT 1 cut(s) 516
AsuC2I CCSGG 2 cut(s) 1359, 1568
AsuHPI GGTGA 4 cut(s) 117, 169, 781, 1057
BaeGI GKGCMC 1 cut(s) 1276
BalI TGGCCA 2 cut(s) 275, 1016
BanI GGYRCC 2 cut(s) 198, 869
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 1415
Bbv12I GWGCWC 4 cut(s) 55, 64, 578, 811
BbvI GCAGC 6 cut(s) 57, 60, 707, 974, 1120, 1291
BccI CCATC 6 cut(s) 148, 313, 356, 379, 608, 1228
BciT130I CCWGG 3 cut(s) 286, 571, 1201
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 2 cut(s) 1359, 1568
BcoDI GTCTC 3 cut(s) 42, 283, 1166
BfaI CTAG 3 cut(s) 42, 195, 849
BisI GCNGC 8 cut(s) 71, 74, 696, 755, 832, 963, 1134, 1305
BlsI GCNGC 8 cut(s) 72, 75, 697, 756, 833, 964, 1135, 1306
BmcAI AGTACT 2 cut(s) 587, 1156
Bme1390I CCNGG 5 cut(s) 286, 571, 1201, 1359, 1568
BmiI GGNNCC 2 cut(s) 200, 871
BmrFI CCNGG 5 cut(s) 286, 571, 1201, 1359, 1568
BmrI ACTGGG 3 cut(s) 65, 90, 1148
BmsI GCATC 4 cut(s) 388, 808, 879, 1396
BmuI ACTGGG 3 cut(s) 65, 90, 1148
BpiI GAAGAC 1 cut(s) 1415
BpmI CTGGAG 5 cut(s) 307, 592, 677, 905, 1092
Bpu10I CCTNAGC 1 cut(s) 1539
BpuMI CCSGG 2 cut(s) 1359, 1568
BsaBI GATNNNNATC 1 cut(s) 504
BsaI GGTCTC 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 1566
BsaXI ACNNNNNCTCC 2 cut(s) 1075, 1105
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 1023, 1427
Bse1I ACTGG 5 cut(s) 34, 60, 96, 653, 1143
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 3 cut(s) 286, 571, 1201
BseDI CCNNGG 1 cut(s) 1566
BseGI GGATG 5 cut(s) 390, 535, 600, 747, 823
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 3 cut(s) 47, 1023, 1427
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 5 cut(s) 34, 60, 96, 653, 1143
BseRI GAGGAG 3 cut(s) 60, 63, 1096
BseSI GKGCMC 1 cut(s) 1276
BseXI GCAGC 6 cut(s) 57, 60, 707, 974, 1120, 1291
BsgI GTGCAG 2 cut(s) 1119, 1290
BshFI GGCC 4 cut(s) 275, 1016, 1420, 1500
BshNI GGYRCC 2 cut(s) 198, 869
BsiHKAI GWGCWC 4 cut(s) 55, 64, 578, 811
BsiSI CCGG 2 cut(s) 1358, 1568
BslFI GGGAC 1 cut(s) 779
BslI CCNNNNNNNGG 3 cut(s) 47, 1023, 1427
BsmAI GTCTC 3 cut(s) 42, 283, 1166
BsmBI CGTCTC 1 cut(s) 1166
BsmFI GGGAC 1 cut(s) 779
BsmI GAATGC 2 cut(s) 172, 430
BsnI GGCC 4 cut(s) 275, 1016, 1420, 1500
Bso31I GGTCTC 1 cut(s) 283
Bsp1286I GDGCHC 5 cut(s) 55, 64, 578, 811, 1276
Bsp143I GATC 2 cut(s) 505, 1521
BspACI CCGC 3 cut(s) 754, 832, 917
BspANI GGCC 4 cut(s) 275, 1016, 1420, 1500
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 200, 871
BspT107I GGYRCC 2 cut(s) 198, 869
BspTNI GGTCTC 1 cut(s) 283
BsrI ACTGG 5 cut(s) 34, 60, 96, 653, 1143
BssECI CCNNGG 1 cut(s) 1566
BssMI GATC 2 cut(s) 505, 1521
Bst2UI CCWGG 3 cut(s) 286, 571, 1201
Bst4CI ACNGT 3 cut(s) 252, 1154, 1334
Bst6I CTCTTC 3 cut(s) 1107, 1233, 1451
BstAPI GCANNNNNTGC 2 cut(s) 59, 701
BstC8I GCNNGC 3 cut(s) 1008, 1131, 1328
BstDEI CTNAG 3 cut(s) 212, 336, 1539
BstENI CCTNNNNNAGG 1 cut(s) 45
BstF5I GGATG 5 cut(s) 390, 535, 600, 747, 823
BstKTI GATC 2 cut(s) 508, 1524
BstMAI GTCTC 3 cut(s) 42, 283, 1166
BstMBI GATC 2 cut(s) 505, 1521
BstMWI GCNNNNNNNGC 8 cut(s) 59, 281, 376, 605, 701, 889, 971, 1205
BstNI CCWGG 3 cut(s) 286, 571, 1201
BstNSI RCATGY 3 cut(s) 1330, 1369, 1579
BstSCI CCNGG 5 cut(s) 284, 569, 1199, 1357, 1566
BstSLI GKGCMC 1 cut(s) 1276
BstV1I GCAGC 6 cut(s) 57, 60, 707, 974, 1120, 1291
BstV2I GAAGAC 1 cut(s) 1415
BsuRI GGCC 4 cut(s) 275, 1016, 1420, 1500
BtsCI GGATG 5 cut(s) 390, 535, 600, 747, 823
BtsIMutI CAGTG 4 cut(s) 53, 1136, 1229, 1339
Cac8I GCNNGC 3 cut(s) 1008, 1131, 1328
CaiI CAGNNNCTG 2 cut(s) 407, 629
Csp6I GTAC 5 cut(s) 19, 31, 586, 1155, 1186
CviAII CATG 9 cut(s) 119, 487, 635, 1327, 1366, 1426, 1502, 1576, 1587
CviQI GTAC 5 cut(s) 19, 31, 586, 1155, 1186
DdeI CTNAG 3 cut(s) 212, 336, 1539
DpnI GATC 2 cut(s) 507, 1523
DpnII GATC 2 cut(s) 505, 1521
DraIII CACNNNGTG 2 cut(s) 59, 1183
EaeI YGGCCR 2 cut(s) 273, 1014
Eam1104I CTCTTC 3 cut(s) 1107, 1233, 1451
EarI CTCTTC 3 cut(s) 1107, 1233, 1451
Eco31I GGTCTC 1 cut(s) 283
Eco32I GATATC 1 cut(s) 842
Eco57I CTGAAG 4 cut(s) 222, 624, 662, 1245
EcoNI CCTNNNNNAGG 1 cut(s) 45
EcoRII CCWGG 3 cut(s) 284, 569, 1199
EcoRV GATATC 1 cut(s) 842
EcoT22I ATGCAT 1 cut(s) 1378
Esp3I CGTCTC 1 cut(s) 1166
FaeI CATG 9 cut(s) 122, 490, 638, 1330, 1369, 1429, 1505, 1579, 1590
FalI AAGNNNNNCTT 2 cut(s) 1450, 1482
FaqI GGGAC 1 cut(s) 779
FatI CATG 9 cut(s) 118, 486, 634, 1326, 1365, 1425, 1501, 1575, 1586
FauI CCCGC 1 cut(s) 910
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 8 cut(s) 71, 74, 696, 755, 832, 963, 1134, 1305
FokI GGATG 5 cut(s) 397, 542, 587, 734, 830
Fsp4HI GCNGC 8 cut(s) 71, 74, 696, 755, 832, 963, 1134, 1305
FspBI CTAG 3 cut(s) 42, 195, 849
GluI GCNGC 8 cut(s) 71, 74, 696, 755, 832, 963, 1134, 1305
GsuI CTGGAG 5 cut(s) 307, 592, 677, 905, 1092
HaeIII GGCC 4 cut(s) 275, 1016, 1420, 1500
HapII CCGG 2 cut(s) 1358, 1568
Hin1II CATG 9 cut(s) 122, 490, 638, 1330, 1369, 1429, 1505, 1579, 1590
HincII GTYRAC 1 cut(s) 1442
HindII GTYRAC 1 cut(s) 1442
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 6 cut(s) 342, 439, 457, 473, 1084, 1105
HpaII CCGG 2 cut(s) 1358, 1568
HphI GGTGA 4 cut(s) 117, 169, 781, 1057
Hpy166II GTNNAC 3 cut(s) 264, 1186, 1442
Hpy188III TCNNGA 3 cut(s) 359, 656, 1109
Hpy8I GTNNAC 3 cut(s) 264, 1186, 1442
HpyCH4III ACNGT 3 cut(s) 252, 1154, 1334
HpyCH4IV ACGT 2 cut(s) 1270, 1320
HpyF10VI GCNNNNNNNGC 8 cut(s) 59, 281, 376, 605, 701, 889, 971, 1205
HpyF3I CTNAG 3 cut(s) 212, 336, 1539
HpySE526I ACGT 2 cut(s) 1270, 1320
Hsp92II CATG 9 cut(s) 122, 490, 638, 1330, 1369, 1429, 1505, 1579, 1590
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 2 cut(s) 505, 1521
LmnI GCTCC 4 cut(s) 50, 485, 573, 860
Lsp1109I GCAGC 6 cut(s) 57, 60, 707, 974, 1120, 1291
LweI GCATC 4 cut(s) 388, 808, 879, 1396
MaeI CTAG 3 cut(s) 42, 195, 849
MaeII ACGT 2 cut(s) 1270, 1320
MaeIII GTNAC 1 cut(s) 1334
MalI GATC 2 cut(s) 507, 1523
MboI GATC 2 cut(s) 505, 1521
MboII GAAGA 8 cut(s) 254, 257, 1124, 1250, 1251, 1277, 1420, 1438
MfeI CAATTG 2 cut(s) 309, 699
MhlI GDGCHC 5 cut(s) 55, 64, 578, 811, 1276
MlsI TGGCCA 2 cut(s) 275, 1016
MluCI AATT 8 cut(s) 309, 690, 699, 772, 923, 1061, 1210, 1278
MluNI TGGCCA 2 cut(s) 275, 1016
MlyI GAGTC 2 cut(s) 1093, 1099
MnlI CCTC 7 cut(s) 38, 41, 331, 359, 375, 1074, 1184
Mox20I TGGCCA 2 cut(s) 275, 1016
Mph1103I ATGCAT 1 cut(s) 1378
MscI TGGCCA 2 cut(s) 275, 1016
MseI TTAA 7 cut(s) 516, 546, 618, 785, 1053, 1281, 1452
Msp20I TGGCCA 2 cut(s) 275, 1016
MspA1I CMGCKG 1 cut(s) 754
MspI CCGG 2 cut(s) 1358, 1568
MspR9I CCNGG 5 cut(s) 286, 571, 1201, 1359, 1568
MunI CAATTG 2 cut(s) 309, 699
Mva1269I GAATGC 2 cut(s) 172, 430
MvaI CCWGG 3 cut(s) 286, 571, 1201
MwoI GCNNNNNNNGC 8 cut(s) 59, 281, 376, 605, 701, 889, 971, 1205
NciI CCSGG 2 cut(s) 1359, 1568
NdeII GATC 2 cut(s) 505, 1521
NlaIII CATG 9 cut(s) 122, 490, 638, 1330, 1369, 1429, 1505, 1579, 1590
NlaIV GGNNCC 2 cut(s) 200, 871
NmuCI GTSAC 1 cut(s) 1334
NsiI ATGCAT 1 cut(s) 1378
NspI RCATGY 3 cut(s) 1330, 1369, 1579
PaeI GCATGC 1 cut(s) 1330
PctI GAATGC 2 cut(s) 172, 430
PfeI GAWTC 4 cut(s) 342, 439, 457, 473
PfoI TCCNGGA 2 cut(s) 569, 1357
PkrI GCNGC 8 cut(s) 72, 75, 697, 756, 833, 964, 1135, 1306
PleI GAGTC 2 cut(s) 1092, 1099
PpsI GAGTC 2 cut(s) 1092, 1099
PshBI ATTAAT 1 cut(s) 516
Psp6I CCWGG 3 cut(s) 284, 569, 1199
PspGI CCWGG 3 cut(s) 284, 569, 1199
PspN4I GGNNCC 2 cut(s) 200, 871
PstNI CAGNNNCTG 2 cut(s) 407, 629
RsaI GTAC 5 cut(s) 20, 32, 587, 1156, 1187
RsaNI GTAC 5 cut(s) 19, 31, 586, 1155, 1186
SaqAI TTAA 7 cut(s) 516, 546, 618, 785, 1053, 1281, 1452
SatI GCNGC 8 cut(s) 71, 74, 696, 755, 832, 963, 1134, 1305
Sau3AI GATC 2 cut(s) 505, 1521
ScaI AGTACT 2 cut(s) 587, 1156
SchI GAGTC 2 cut(s) 1093, 1099
ScrFI CCNGG 5 cut(s) 286, 571, 1201, 1359, 1568
SduI GDGCHC 5 cut(s) 55, 64, 578, 811, 1276
SfaNI GCATC 4 cut(s) 388, 808, 879, 1396
SphI GCATGC 1 cut(s) 1330
Sse9I AATT 8 cut(s) 309, 690, 699, 772, 923, 1061, 1210, 1278
SsiI CCGC 3 cut(s) 754, 832, 917
SspMI CTAG 3 cut(s) 42, 195, 849
StyD4I CCNGG 5 cut(s) 284, 569, 1199, 1357, 1566
TaaI ACNGT 3 cut(s) 252, 1154, 1334
TaiI ACGT 2 cut(s) 1273, 1323
TaqI TCGA 5 cut(s) 164, 360, 838, 1390, 1547
TasI AATT 8 cut(s) 309, 690, 699, 772, 923, 1061, 1210, 1278
TatI WGTACW 2 cut(s) 585, 1154
TauI GCSGC 2 cut(s) 757, 834
TfiI GAWTC 4 cut(s) 342, 439, 457, 473
Tru1I TTAA 7 cut(s) 516, 546, 618, 785, 1053, 1281, 1452
Tru9I TTAA 7 cut(s) 516, 546, 618, 785, 1053, 1281, 1452
TscAI CASTG 4 cut(s) 60, 1143, 1236, 1339
TseFI GTSAC 1 cut(s) 1334
TseI GCWGC 6 cut(s) 70, 73, 695, 962, 1133, 1304
Tsp45I GTSAC 1 cut(s) 1334
TspDTI ATGAA 6 cut(s) 309, 334, 475, 502, 623, 1518
TspGWI ACGGA 2 cut(s) 702, 1203
TspRI CASTG 4 cut(s) 60, 1143, 1236, 1339
VspI ATTAAT 1 cut(s) 516
XagI CCTNNNNNAGG 1 cut(s) 45
XapI RAATTY 3 cut(s) 772, 923, 1210
XceI RCATGY 3 cut(s) 1330, 1369, 1579
XcmI CCANNNNNNNNNTGG 2 cut(s) 652, 880
XspI CTAG 3 cut(s) 42, 195, 849
ZrmI AGTACT 2 cut(s) 587, 1156
Zsp2I ATGCAT 1 cut(s) 1378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.