RLG00000017876

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014966
Physical Location & Seq
Reverse (-)
22526984 .. 22532498
5515 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017876

Sequence Viewer

Length: 1206 bp
ATGGGGATATGTGATGCAGTCGCTGTTGCTAAAATACTGAATGCAACTCTTGTGATTCCATACCTTGAAGTGAATCCTGTTTGGCGAGATTCAAGCTCCTTCATGGATATATTTGATGTTGATCATTTCATTAATGTATTGAAGGATGATATATCCATAGTTAAAGAGCTACCTTCGGACTTCTCCTGGAGCACAAGGGAGTACTATGCAACAGCCATCCGAGCTACGAGAGTTAAGACAGCACCTGTTCATGCTTCAGCCAACTGGTATCTGGAGAATGTATTGCCCGTACTTCAGAGCTACGGAATTGCAGCAATTGCCCCTTTCTCTCATCGGCTGACATTTGATACCTTGCCTATGGACATCCAGCGGCTACGCTGTAAGGTGAATTTTCAAGCATTAAGTTTTGTCCCACATATAAGAGCACTTGGGGATGCTCTTATCAGCCGCCTTCGATATCCTTCTAGCAAAAGTGGAGCAATCAGCACCAATCACTTGCTGGAGATGCCTAATGTAAAAGGCAAACAAGGGGCGGGGAAATTTGTTGTTCTACATCTACGATTTGACAAGGATATGGCAGCTCATTCAGCTTGTGATTTTGGTGGTGGAAAAGCTGAAAAGCTGGCTCTGGCCAAATACAGGCAAGTGCTTTGGCAGGGAAGGGTTCTTAACTCCCAATTCACCGACAAAGAGTTGAGGAGTCAGGGTCGTTGTCCATTGACTCCAGAAGAGATTGGGTTGGTGCTTGCTGCACTGGGATTTGACAACAGTACTCGTCTCTATCTTGCTTCCCACAAGGTATACGGAGGGGAAGCTAGGATTGCAAATTTGCGACACTTGTTTCCACTGATGGAAGACAAAAAGAGCCTTGCTTCTTCAGAAGAACGTGCCCAAATTAAAGGGAAGGCTTCTCTGTTAGCTGCACTTGATTACTACGTCAGCATGCACAGCGACATCTTTGTTTCTGCTTCTCCAGGGAACATGCACAATGCATTGGTAGGGCATCGAACTTACGAGAACTTGAAGACGATTAGGCCAAGCATGGGTTTATTGGGTCAACTCTTCCTTAACAAAAGCATTACTTGGTCAGATTTCCAGCAATCAGTAGTAGAAGGCCATGAAAACAGACAAGGACAGATCAGGTTCAGAAAGCCTAAGCAGTCGATATACACTTATCCTGCCCCGGACTGCATGTGCCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

402

Amino Acids

44.82

Weight (kDa)

9.22

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 2 - 338 5.6e-85 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65470
fragaria_vesca FvH4_1g19680 FvH4_1g19680
malus_domestica MD01G1039200.v1.1 MD15G1312400.v1.1
prunus_persica Prupe.6G201400_v2.0.a1 Prupe.6G201400_v2.0.a1
pyrus_communis pycom01g06690
rosa_chinensis RchiOBHm_Chr2g0111231
rosa_laevigata RLG00000017876
rosa_multiflora Rmu_sc0003055.1_g000005
rosa_roxburghii Rroxscaffold_2G00132600
rosa_rugosa Rorug02G0171900 Rorug02G0171900
rosa_samantha Rh2AG225000
rosa_wichuraiana Rw2G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 801
AciI CCGC 3 cut(s) 370, 448, 533
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 3 cut(s) 388, 539, 826
AcuI CTGAAG 3 cut(s) 240, 278, 861
AfaI GTAC 3 cut(s) 203, 291, 772
AfiI CCNNNNNNNGG 2 cut(s) 639, 1043
AgsI TTSAA 5 cut(s) 68, 93, 142, 395, 1024
AjnI CCWGG 2 cut(s) 185, 973
Alw21I GWGCWC 2 cut(s) 194, 427
Alw26I GTCTC 1 cut(s) 782
AlwNI CAGNNNCTG 2 cut(s) 23, 245
AoxI GGCC 3 cut(s) 630, 1034, 1114
ApeKI GCWGC 4 cut(s) 311, 578, 749, 920
ApoI RAATTY 3 cut(s) 388, 539, 826
AseI ATTAAT 1 cut(s) 132
AsuC2I CCSGG 1 cut(s) 1184
AsuHPI GGTGA 2 cut(s) 397, 673
BaeGI GKGCMC 1 cut(s) 892
BalI TGGCCA 1 cut(s) 632
BbsI GAAGAC 2 cut(s) 861, 1031
Bbv12I GWGCWC 2 cut(s) 194, 427
BbvI GCAGC 4 cut(s) 323, 590, 736, 907
BccI CCATC 2 cut(s) 224, 844
BciT130I CCWGG 2 cut(s) 187, 975
BclI TGATCA 1 cut(s) 121
BcnI CCSGG 1 cut(s) 1184
BcoDI GTCTC 1 cut(s) 782
BfaI CTAG 2 cut(s) 465, 816
BisI GCNGC 6 cut(s) 312, 371, 448, 579, 750, 921
BlsI GCNGC 6 cut(s) 313, 372, 449, 580, 751, 922
BmcAI AGTACT 2 cut(s) 203, 772
Bme1390I CCNGG 3 cut(s) 187, 975, 1184
BmrFI CCNGG 3 cut(s) 187, 975, 1184
BmrI ACTGGG 1 cut(s) 764
BmsI GCATC 4 cut(s) 4, 424, 495, 1012
BmuI ACTGGG 1 cut(s) 764
BpiI GAAGAC 2 cut(s) 861, 1031
BpmI CTGGAG 5 cut(s) 208, 293, 521, 708, 957
Bpu10I CCTNAGC 1 cut(s) 1155
BpuMI CCSGG 1 cut(s) 1184
BsaBI GATNNNNATC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 974, 1182
BsaXI ACNNNNNCTCC 2 cut(s) 691, 721
Bsc4I CCNNNNNNNGG 2 cut(s) 639, 1043
Bse1I ACTGG 2 cut(s) 269, 759
Bse8I GATNNNNATC 1 cut(s) 120
BseBI CCWGG 2 cut(s) 187, 975
BseDI CCNNGG 2 cut(s) 974, 1182
BseGI GGATG 4 cut(s) 151, 216, 363, 439
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 639, 1043
BseNI ACTGG 2 cut(s) 269, 759
BseRI GAGGAG 1 cut(s) 712
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 4 cut(s) 323, 590, 736, 907
BsgI GTGCAG 2 cut(s) 735, 906
BshFI GGCC 3 cut(s) 632, 1036, 1116
BsiHKAI GWGCWC 2 cut(s) 194, 427
BsiSI CCGG 1 cut(s) 1184
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 2 cut(s) 639, 1043
BsmAI GTCTC 1 cut(s) 782
BsmBI CGTCTC 1 cut(s) 782
BsmFI GGGAC 1 cut(s) 395
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 3 cut(s) 632, 1036, 1116
Bsp1286I GDGCHC 3 cut(s) 194, 427, 892
Bsp143I GATC 2 cut(s) 121, 1137
BspACI CCGC 3 cut(s) 370, 448, 533
BspANI GGCC 3 cut(s) 632, 1036, 1116
BsrI ACTGG 2 cut(s) 269, 759
BssECI CCNNGG 2 cut(s) 974, 1182
BssMI GATC 2 cut(s) 121, 1137
BssNAI GTATAC 1 cut(s) 802
Bst1107I GTATAC 1 cut(s) 802
Bst2UI CCWGG 2 cut(s) 187, 975
Bst4CI ACNGT 1 cut(s) 770
Bst6I CTCTTC 2 cut(s) 723, 1067
BstAPI GCANNNNNTGC 1 cut(s) 317
BstC8I GCNNGC 3 cut(s) 624, 747, 944
BstDEI CTNAG 1 cut(s) 1155
BstF5I GGATG 4 cut(s) 151, 216, 363, 439
BstKTI GATC 2 cut(s) 124, 1140
BstMAI GTCTC 1 cut(s) 782
BstMBI GATC 2 cut(s) 121, 1137
BstMWI GCNNNNNNNGC 6 cut(s) 221, 317, 505, 587, 821, 948
BstNI CCWGG 2 cut(s) 187, 975
BstNSI RCATGY 3 cut(s) 946, 985, 1195
BstSCI CCNGG 3 cut(s) 185, 973, 1182
BstSLI GKGCMC 1 cut(s) 892
BstV1I GCAGC 4 cut(s) 323, 590, 736, 907
BstV2I GAAGAC 2 cut(s) 861, 1031
BstZ17I GTATAC 1 cut(s) 802
BsuRI GGCC 3 cut(s) 632, 1036, 1116
BtsCI GGATG 4 cut(s) 151, 216, 363, 439
BtsIMutI CAGTG 2 cut(s) 752, 845
Cac8I GCNNGC 3 cut(s) 624, 747, 944
CaiI CAGNNNCTG 2 cut(s) 23, 245
Csp6I GTAC 3 cut(s) 202, 290, 771
CviAII CATG 8 cut(s) 103, 251, 943, 982, 1042, 1118, 1192, 1203
CviQI GTAC 3 cut(s) 202, 290, 771
DdeI CTNAG 1 cut(s) 1155
DpnI GATC 2 cut(s) 123, 1139
DpnII GATC 2 cut(s) 121, 1137
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 723, 1067
EarI CTCTTC 2 cut(s) 723, 1067
Eco32I GATATC 1 cut(s) 458
Eco57I CTGAAG 3 cut(s) 240, 278, 861
EcoRII CCWGG 2 cut(s) 185, 973
EcoRV GATATC 1 cut(s) 458
EcoT22I ATGCAT 1 cut(s) 994
Esp3I CGTCTC 1 cut(s) 782
FaeI CATG 8 cut(s) 106, 254, 946, 985, 1045, 1121, 1195, 1206
FalI AAGNNNNNCTT 2 cut(s) 1066, 1098
FaqI GGGAC 1 cut(s) 395
FatI CATG 8 cut(s) 102, 250, 942, 981, 1041, 1117, 1191, 1202
FauI CCCGC 1 cut(s) 526
FbaI TGATCA 1 cut(s) 121
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 6 cut(s) 312, 371, 448, 579, 750, 921
FokI GGATG 4 cut(s) 158, 203, 350, 446
Fsp4HI GCNGC 6 cut(s) 312, 371, 448, 579, 750, 921
FspBI CTAG 2 cut(s) 465, 816
GluI GCNGC 6 cut(s) 312, 371, 448, 579, 750, 921
GsuI CTGGAG 5 cut(s) 208, 293, 521, 708, 957
HaeIII GGCC 3 cut(s) 632, 1036, 1116
HapII CCGG 1 cut(s) 1184
Hin1II CATG 8 cut(s) 106, 254, 946, 985, 1045, 1121, 1195, 1206
HincII GTYRAC 1 cut(s) 1058
HindII GTYRAC 1 cut(s) 1058
HinfI GANTC 5 cut(s) 55, 73, 89, 700, 721
HpaII CCGG 1 cut(s) 1184
HphI GGTGA 2 cut(s) 397, 673
Hpy166II GTNNAC 2 cut(s) 802, 1058
Hpy188I TCNGA 6 cut(s) 178, 221, 297, 880, 1090, 1148
Hpy188III TCNNGA 2 cut(s) 272, 725
Hpy8I GTNNAC 2 cut(s) 802, 1058
HpyAV CCTTC 8 cut(s) 109, 136, 183, 461, 471, 654, 898, 1106
HpyCH4III ACNGT 1 cut(s) 770
HpyCH4IV ACGT 2 cut(s) 886, 936
HpyF10VI GCNNNNNNNGC 6 cut(s) 221, 317, 505, 587, 821, 948
HpyF3I CTNAG 1 cut(s) 1155
HpySE526I ACGT 2 cut(s) 886, 936
Hsp92II CATG 8 cut(s) 106, 254, 946, 985, 1045, 1121, 1195, 1206
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 2 cut(s) 121, 1137
LmnI GCTCC 3 cut(s) 101, 189, 476
Lsp1109I GCAGC 4 cut(s) 323, 590, 736, 907
LweI GCATC 4 cut(s) 4, 424, 495, 1012
MaeI CTAG 2 cut(s) 465, 816
MaeII ACGT 2 cut(s) 886, 936
MalI GATC 2 cut(s) 123, 1139
MboI GATC 2 cut(s) 121, 1137
MboII GAAGA 6 cut(s) 740, 866, 867, 893, 1036, 1054
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 3 cut(s) 194, 427, 892
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 7 cut(s) 306, 315, 388, 539, 677, 826, 894
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 2 cut(s) 709, 715
MnlI CCTC 2 cut(s) 690, 800
Mox20I TGGCCA 1 cut(s) 632
Mph1103I ATGCAT 1 cut(s) 994
MscI TGGCCA 1 cut(s) 632
MseI TTAA 7 cut(s) 132, 162, 234, 401, 669, 897, 1068
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 1 cut(s) 370
MspI CCGG 1 cut(s) 1184
MspR9I CCNGG 3 cut(s) 187, 975, 1184
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 2 cut(s) 187, 975
MwoI GCNNNNNNNGC 6 cut(s) 221, 317, 505, 587, 821, 948
NciI CCSGG 1 cut(s) 1184
NdeII GATC 2 cut(s) 121, 1137
NlaIII CATG 8 cut(s) 106, 254, 946, 985, 1045, 1121, 1195, 1206
NsiI ATGCAT 1 cut(s) 994
NspI RCATGY 3 cut(s) 946, 985, 1195
PaeI GCATGC 1 cut(s) 946
PctI GAATGC 1 cut(s) 46
PfeI GAWTC 3 cut(s) 55, 73, 89
PfoI TCCNGGA 1 cut(s) 185
PkrI GCNGC 6 cut(s) 313, 372, 449, 580, 751, 922
PleI GAGTC 2 cut(s) 708, 715
PpsI GAGTC 2 cut(s) 708, 715
PshBI ATTAAT 1 cut(s) 132
Psp6I CCWGG 2 cut(s) 185, 973
PspGI CCWGG 2 cut(s) 185, 973
PstNI CAGNNNCTG 2 cut(s) 23, 245
RsaI GTAC 3 cut(s) 203, 291, 772
RsaNI GTAC 3 cut(s) 202, 290, 771
SaqAI TTAA 7 cut(s) 132, 162, 234, 401, 669, 897, 1068
SatI GCNGC 6 cut(s) 312, 371, 448, 579, 750, 921
Sau3AI GATC 2 cut(s) 121, 1137
ScaI AGTACT 2 cut(s) 203, 772
SchI GAGTC 2 cut(s) 709, 715
ScrFI CCNGG 3 cut(s) 187, 975, 1184
SduI GDGCHC 3 cut(s) 194, 427, 892
SfaNI GCATC 4 cut(s) 4, 424, 495, 1012
SphI GCATGC 1 cut(s) 946
Sse9I AATT 7 cut(s) 306, 315, 388, 539, 677, 826, 894
SsiI CCGC 3 cut(s) 370, 448, 533
SspMI CTAG 2 cut(s) 465, 816
StyD4I CCNGG 3 cut(s) 185, 973, 1182
TaaI ACNGT 1 cut(s) 770
TaiI ACGT 2 cut(s) 889, 939
TaqI TCGA 3 cut(s) 454, 1006, 1163
TasI AATT 7 cut(s) 306, 315, 388, 539, 677, 826, 894
TatI WGTACW 2 cut(s) 201, 770
TauI GCSGC 2 cut(s) 373, 450
TfiI GAWTC 3 cut(s) 55, 73, 89
Tru1I TTAA 7 cut(s) 132, 162, 234, 401, 669, 897, 1068
Tru9I TTAA 7 cut(s) 132, 162, 234, 401, 669, 897, 1068
TscAI CASTG 2 cut(s) 759, 852
TseI GCWGC 4 cut(s) 311, 578, 749, 920
TspDTI ATGAA 4 cut(s) 91, 118, 239, 1134
TspGWI ACGGA 2 cut(s) 318, 819
TspRI CASTG 2 cut(s) 759, 852
VspI ATTAAT 1 cut(s) 132
XapI RAATTY 3 cut(s) 388, 539, 826
XceI RCATGY 3 cut(s) 946, 985, 1195
XcmI CCANNNNNNNNNTGG 2 cut(s) 268, 496
XmiI GTMKAC 1 cut(s) 801
XspI CTAG 2 cut(s) 465, 816
ZrmI AGTACT 2 cut(s) 203, 772
Zsp2I ATGCAT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.