FvH4_1g19680

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014966
Physical Location & Seq
Reverse (-)
11894865 .. 11901230
6366 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19680.t2

Sequence Viewer

Length: 1590 bp
ATGGGGCTGAAGCTTCAGTACCAGAATGAGTACCAGTCTCCTAGAGGAGGAGCAGTTGGTGCTCTTTTGCTGCTGCTCTTTCCCATTTTCTTGCCCAGTTTGTTCAGCCCTTTTGGTCATGCTTCACCTTCAATGCTCTCGGAATGGAATGTGATGGTGAAGTCAAATGCACAAGAAGGGAATGTTCCAAAATCTAGGCACCAACGCCTTCTCAAGAGTGCTTTACAACGCCAAACTTCAGAAGAAGAACTGTCAGAGTTGTGGACACCATTGGCTGATCAAGCTTGGAAACCTTGCATAGAACCATCAATTGCCCCCTCATTACCAGAGAAATCTGAGGGATTCATCCAAGTATTTCTCGATGGAGGGTTGAACCAGCAGAGGATGGGGATATGTGATGCAGTCGCTGTTGCTAAAATACTGAATGCAACACTTGTGATTCCATACCTTGAAGTGAATCCTGTGTGGCGAGATTCAAGCTCCTTCATGGATATATTTGATGTTGATCATTTCATTAATGTATTGAAGGATGATATATCCATAGTTAAAGAGCTACCTTCAGACTTCTCCTGGAGCACAAGGGAGTACTATGCAACAGCCATCCGAGCTACGAGAGTTAAGACAGCACCTGTTCATGCTTCAGCCAACTGGTATCTGGAGAATGTTTTGCCTGTACTTCAGAGCTATGGAATTGCAGCAATTGCCCCCTTTTCTCATCGGCTGACTTTTGATACCTTGCCTATGGACATCCAGCGGCTACGCTGTAAGGTGAACTTTCAAGCATTAAGTTTTGTCCCACATATAAGAGCACTTGGAGATGCTCTAATTAGCCGCCTTCGATATCCATCTGGTAAAAGTGGACCAATTGGCACCAATCATCTGCTGGAGTTGCCTAATGTGAAAGGCAAACAAGGGGAAGGAAAATTTGTTGTTCTACATCTACGATTTGACAAGGATATGGCAGCTCATTCAGCTTGTGATTTTGGTGGTGGAAAAGCTGAAAAGCTGGCTCTAGCCAAATACCGACAAGTGCTTTGGCAGGGAAGGGTCCTTAACTCCCAATTCACTGACAAAGAGTTGAGGAGTCAGGGTCGTTGTCCATTGACTCCAGAAGAAATTGGGTTGCTGCTTGCTGCATTGGGATTTGACAACAGTACTCGTCTCTATCTTGCTTCGCACAAGGTATACGGTGGGGAAGCTAGAATTGAAAATTTGCGACACTTGTTTCCACTGATGGAAGACAAAAAGAGCCTTGCTTCTTCAGAAGAACGTGCCCTAATCAAAGGAAAGGCTTCTCTGTTAGCTGCGCTAGATTACTACGTTAGCATGCACAGTGATATCTTCGTTTCTGCTTCTCCTGGAAATATGCACAATGCATTGGTAGGGCATCGAACTTACGAGAACTTGAAGACGATTAGGCCAAGCATGGGTTTATTGGGTCAACTCTTCCTTAACAAAAGCATTACTTGGTCAGATTTCCAGCAATCAGTAGTAGAAGGCCATGAAAACAGACAAGGACAGATCAGGTTCAGAAAGCCTAAGCAGTCGATATACACTTATCCTGCCCCGGACTGCATGTGCCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

530

Amino Acids

59.15

Weight (kDa)

8.86

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 66 - 415 6.4e-91 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014966)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65470
fragaria_vesca FvH4_1g19680 FvH4_1g19680
malus_domestica MD01G1039200.v1.1 MD15G1312400.v1.1
prunus_persica Prupe.6G201400_v2.0.a1 Prupe.6G201400_v2.0.a1
pyrus_communis pycom01g06690
rosa_chinensis RchiOBHm_Chr2g0111231
rosa_laevigata RLG00000017876
rosa_multiflora Rmu_sc0003055.1_g000005
rosa_roxburghii Rroxscaffold_2G00132600
rosa_rugosa Rorug02G0171900 Rorug02G0171900
rosa_samantha Rh2AG225000
rosa_wichuraiana Rw2G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 198, 869
AccI GTMKAC 1 cut(s) 1185
AciI CCGC 2 cut(s) 754, 832
AcsI RAATTY 2 cut(s) 923, 1210
AcuI CTGAAG 6 cut(s) 29, 222, 543, 624, 662, 1245
AfaI GTAC 5 cut(s) 20, 32, 587, 675, 1156
AfiI CCNNNNNNNGG 2 cut(s) 47, 1427
AgsI TTSAA 8 cut(s) 132, 373, 452, 477, 526, 779, 1208, 1408
AjnI CCWGG 2 cut(s) 569, 1357
Alw21I GWGCWC 3 cut(s) 64, 578, 811
Alw26I GTCTC 2 cut(s) 42, 1166
AlwNI CAGNNNCTG 2 cut(s) 407, 629
AoxI GGCC 2 cut(s) 1418, 1498
ApeKI GCWGC 7 cut(s) 70, 73, 695, 962, 1126, 1133, 1304
ApoI RAATTY 2 cut(s) 923, 1210
ArsI GACNNNNNNTTYG 2 cut(s) 1017, 1049
AseI ATTAAT 1 cut(s) 516
Asp700I GAANNNNTTC 1 cut(s) 1291
AspLEI GCGC 1 cut(s) 1309
AspS9I GGNCC 2 cut(s) 860, 1048
AsuC2I CCSGG 1 cut(s) 1568
AsuHPI GGTGA 3 cut(s) 117, 169, 781
AvaII GGWCC 2 cut(s) 860, 1048
BaeGI GKGCMC 1 cut(s) 1276
BanI GGYRCC 2 cut(s) 198, 869
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbsI GAAGAC 2 cut(s) 1245, 1415
Bbv12I GWGCWC 3 cut(s) 64, 578, 811
BbvI GCAGC 7 cut(s) 57, 60, 707, 974, 1113, 1120, 1291
BccI CCATC 7 cut(s) 148, 313, 356, 379, 608, 853, 1228
BciT130I CCWGG 2 cut(s) 571, 1359
BclI TGATCA 2 cut(s) 277, 505
BcnI CCSGG 1 cut(s) 1568
BcoDI GTCTC 2 cut(s) 42, 1166
BfaI CTAG 5 cut(s) 42, 195, 1013, 1200, 1310
BisI GCNGC 9 cut(s) 71, 74, 696, 755, 832, 963, 1127, 1134, 1305
BlsI GCNGC 9 cut(s) 72, 75, 697, 756, 833, 964, 1128, 1135, 1306
BmcAI AGTACT 2 cut(s) 587, 1156
Bme1390I CCNGG 3 cut(s) 571, 1359, 1568
Bme18I GGWCC 2 cut(s) 860, 1048
BmgT120I GGNCC 2 cut(s) 860, 1048
BmiI GGNNCC 3 cut(s) 200, 871, 1049
BmrFI CCNGG 3 cut(s) 571, 1359, 1568
BmrI ACTGGG 1 cut(s) 90
BmsI GCATC 3 cut(s) 388, 808, 1396
BmuI ACTGGG 1 cut(s) 90
BpiI GAAGAC 2 cut(s) 1245, 1415
BpmI CTGGAG 4 cut(s) 592, 677, 905, 1092
Bpu10I CCTNAGC 1 cut(s) 1539
BpuEI CTTGAG 1 cut(s) 197
BpuMI CCSGG 1 cut(s) 1568
BsaBI GATNNNNATC 2 cut(s) 504, 844
BsaJI CCNNGG 1 cut(s) 1566
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 1075, 1105
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 1427
Bse1I ACTGG 3 cut(s) 34, 96, 653
Bse8I GATNNNNATC 2 cut(s) 504, 844
BseBI CCWGG 2 cut(s) 571, 1359
BseDI CCNNGG 1 cut(s) 1566
BseGI GGATG 5 cut(s) 345, 390, 535, 600, 747
BseJI GATNNNNATC 2 cut(s) 504, 844
BseLI CCNNNNNNNGG 2 cut(s) 47, 1427
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 3 cut(s) 34, 96, 653
BseRI GAGGAG 3 cut(s) 60, 63, 1096
BseSI GKGCMC 1 cut(s) 1276
BseXI GCAGC 7 cut(s) 57, 60, 707, 974, 1113, 1120, 1291
BshFI GGCC 2 cut(s) 1420, 1500
BshNI GGYRCC 2 cut(s) 198, 869
BsiHKAI GWGCWC 3 cut(s) 64, 578, 811
BsiSI CCGG 1 cut(s) 1568
BslFI GGGAC 1 cut(s) 779
BslI CCNNNNNNNGG 2 cut(s) 47, 1427
BsmAI GTCTC 2 cut(s) 42, 1166
BsmBI CGTCTC 1 cut(s) 1166
BsmFI GGGAC 1 cut(s) 779
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 2 cut(s) 1420, 1500
Bsp1286I GDGCHC 4 cut(s) 64, 578, 811, 1276
Bsp143I GATC 3 cut(s) 277, 505, 1521
BspACI CCGC 2 cut(s) 754, 832
BspANI GGCC 2 cut(s) 1420, 1500
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 3 cut(s) 200, 871, 1049
BspT107I GGYRCC 2 cut(s) 198, 869
BsrI ACTGG 3 cut(s) 34, 96, 653
BssECI CCNNGG 1 cut(s) 1566
BssMI GATC 3 cut(s) 277, 505, 1521
BssNAI GTATAC 1 cut(s) 1186
Bst1107I GTATAC 1 cut(s) 1186
Bst2UI CCWGG 2 cut(s) 571, 1359
Bst4CI ACNGT 4 cut(s) 252, 1154, 1190, 1334
Bst6I CTCTTC 1 cut(s) 1451
BstAPI GCANNNNNTGC 2 cut(s) 59, 701
BstC8I GCNNGC 3 cut(s) 1008, 1131, 1328
BstDEI CTNAG 2 cut(s) 336, 1539
BstENI CCTNNNNNAGG 1 cut(s) 45
BstF5I GGATG 5 cut(s) 345, 390, 535, 600, 747
BstHHI GCGC 1 cut(s) 1309
BstKTI GATC 3 cut(s) 280, 508, 1524
BstMAI GTCTC 2 cut(s) 42, 1166
BstMBI GATC 3 cut(s) 277, 505, 1521
BstMWI GCNNNNNNNGC 6 cut(s) 59, 281, 605, 701, 889, 971
BstNI CCWGG 2 cut(s) 571, 1359
BstNSI RCATGY 2 cut(s) 1330, 1579
BstSCI CCNGG 3 cut(s) 569, 1357, 1566
BstSLI GKGCMC 1 cut(s) 1276
BstV1I GCAGC 7 cut(s) 57, 60, 707, 974, 1113, 1120, 1291
BstV2I GAAGAC 2 cut(s) 1245, 1415
BstZ17I GTATAC 1 cut(s) 1186
BsuRI GGCC 2 cut(s) 1420, 1500
BtsCI GGATG 5 cut(s) 345, 390, 535, 600, 747
BtsIMutI CAGTG 3 cut(s) 1065, 1229, 1339
Cac8I GCNNGC 3 cut(s) 1008, 1131, 1328
CaiI CAGNNNCTG 2 cut(s) 407, 629
CfoI GCGC 1 cut(s) 1309
Cfr13I GGNCC 2 cut(s) 860, 1048
Csp6I GTAC 5 cut(s) 19, 31, 586, 674, 1155
CviAII CATG 8 cut(s) 119, 487, 635, 1327, 1426, 1502, 1576, 1587
CviQI GTAC 5 cut(s) 19, 31, 586, 674, 1155
DdeI CTNAG 2 cut(s) 336, 1539
DpnI GATC 3 cut(s) 279, 507, 1523
DpnII GATC 3 cut(s) 277, 505, 1521
Eam1104I CTCTTC 1 cut(s) 1451
EarI CTCTTC 1 cut(s) 1451
Eco32I GATATC 2 cut(s) 842, 1339
Eco47I GGWCC 2 cut(s) 860, 1048
Eco57I CTGAAG 6 cut(s) 29, 222, 543, 624, 662, 1245
EcoNI CCTNNNNNAGG 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 1048
EcoRII CCWGG 2 cut(s) 569, 1357
EcoRV GATATC 2 cut(s) 842, 1339
EcoT22I ATGCAT 1 cut(s) 1378
Esp3I CGTCTC 1 cut(s) 1166
FaeI CATG 8 cut(s) 122, 490, 638, 1330, 1429, 1505, 1579, 1590
FalI AAGNNNNNCTT 4 cut(s) 758, 790, 1450, 1482
FaqI GGGAC 1 cut(s) 779
FatI CATG 8 cut(s) 118, 486, 634, 1326, 1425, 1501, 1575, 1586
FbaI TGATCA 2 cut(s) 277, 505
FblI GTMKAC 1 cut(s) 1185
Fnu4HI GCNGC 9 cut(s) 71, 74, 696, 755, 832, 963, 1127, 1134, 1305
FokI GGATG 5 cut(s) 332, 397, 542, 587, 734
Fsp4HI GCNGC 9 cut(s) 71, 74, 696, 755, 832, 963, 1127, 1134, 1305
FspBI CTAG 5 cut(s) 42, 195, 1013, 1200, 1310
GlaI GCGC 1 cut(s) 1308
GluI GCNGC 9 cut(s) 71, 74, 696, 755, 832, 963, 1127, 1134, 1305
GsuI CTGGAG 4 cut(s) 592, 677, 905, 1092
HaeIII GGCC 2 cut(s) 1420, 1500
HapII CCGG 1 cut(s) 1568
HhaI GCGC 1 cut(s) 1309
Hin1II CATG 8 cut(s) 122, 490, 638, 1330, 1429, 1505, 1579, 1590
Hin6I GCGC 1 cut(s) 1307
HinP1I GCGC 1 cut(s) 1307
HincII GTYRAC 1 cut(s) 1442
HindII GTYRAC 1 cut(s) 1442
HindIII AAGCTT 2 cut(s) 11, 282
HinfI GANTC 6 cut(s) 342, 439, 457, 473, 1084, 1105
HpaII CCGG 1 cut(s) 1568
HphI GGTGA 3 cut(s) 117, 169, 781
Hpy166II GTNNAC 5 cut(s) 264, 772, 860, 1186, 1442
Hpy188III TCNNGA 4 cut(s) 214, 359, 656, 1109
Hpy8I GTNNAC 5 cut(s) 264, 772, 860, 1186, 1442
HpyCH4III ACNGT 4 cut(s) 252, 1154, 1190, 1334
HpyCH4IV ACGT 2 cut(s) 1270, 1320
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 281, 605, 701, 889, 971
HpyF3I CTNAG 2 cut(s) 336, 1539
HpySE526I ACGT 2 cut(s) 1270, 1320
Hsp92II CATG 8 cut(s) 122, 490, 638, 1330, 1429, 1505, 1579, 1590
HspAI GCGC 1 cut(s) 1307
Ksp22I TGATCA 2 cut(s) 277, 505
Kzo9I GATC 3 cut(s) 277, 505, 1521
LmnI GCTCC 3 cut(s) 50, 485, 573
Lsp1109I GCAGC 7 cut(s) 57, 60, 707, 974, 1113, 1120, 1291
LweI GCATC 3 cut(s) 388, 808, 1396
MaeI CTAG 5 cut(s) 42, 195, 1013, 1200, 1310
MaeII ACGT 2 cut(s) 1270, 1320
MalI GATC 3 cut(s) 279, 507, 1523
MboI GATC 3 cut(s) 277, 505, 1521
MboII GAAGA 9 cut(s) 254, 257, 1124, 1250, 1251, 1277, 1333, 1420, 1438
MfeI CAATTG 3 cut(s) 309, 699, 864
MhlI GDGCHC 4 cut(s) 64, 578, 811, 1276
MlyI GAGTC 2 cut(s) 1093, 1099
MnlI CCTC 7 cut(s) 38, 41, 328, 331, 359, 375, 1074
Mph1103I ATGCAT 1 cut(s) 1378
MroXI GAANNNNTTC 1 cut(s) 1291
MseI TTAA 6 cut(s) 516, 546, 618, 785, 1053, 1452
MspA1I CMGCKG 1 cut(s) 754
MspI CCGG 1 cut(s) 1568
MspR9I CCNGG 3 cut(s) 571, 1359, 1568
MunI CAATTG 3 cut(s) 309, 699, 864
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 2 cut(s) 571, 1359
MwoI GCNNNNNNNGC 6 cut(s) 59, 281, 605, 701, 889, 971
NciI CCSGG 1 cut(s) 1568
NdeII GATC 3 cut(s) 277, 505, 1521
NlaIII CATG 8 cut(s) 122, 490, 638, 1330, 1429, 1505, 1579, 1590
NlaIV GGNNCC 3 cut(s) 200, 871, 1049
NsiI ATGCAT 1 cut(s) 1378
NspI RCATGY 2 cut(s) 1330, 1579
PaeI GCATGC 1 cut(s) 1330
PctI GAATGC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 1291
PfeI GAWTC 4 cut(s) 342, 439, 457, 473
PfoI TCCNGGA 2 cut(s) 569, 1357
PkrI GCNGC 9 cut(s) 72, 75, 697, 756, 833, 964, 1128, 1135, 1306
PleI GAGTC 2 cut(s) 1092, 1099
PpsI GAGTC 2 cut(s) 1092, 1099
PpuMI RGGWCCY 1 cut(s) 1048
PshBI ATTAAT 1 cut(s) 516
Psp5II RGGWCCY 1 cut(s) 1048
Psp6I CCWGG 2 cut(s) 569, 1357
PspGI CCWGG 2 cut(s) 569, 1357
PspN4I GGNNCC 3 cut(s) 200, 871, 1049
PspPI GGNCC 2 cut(s) 860, 1048
PspPPI RGGWCCY 1 cut(s) 1048
PstNI CAGNNNCTG 2 cut(s) 407, 629
RsaI GTAC 5 cut(s) 20, 32, 587, 675, 1156
RsaNI GTAC 5 cut(s) 19, 31, 586, 674, 1155
SaqAI TTAA 6 cut(s) 516, 546, 618, 785, 1053, 1452
SatI GCNGC 9 cut(s) 71, 74, 696, 755, 832, 963, 1127, 1134, 1305
Sau3AI GATC 3 cut(s) 277, 505, 1521
Sau96I GGNCC 2 cut(s) 860, 1048
ScaI AGTACT 2 cut(s) 587, 1156
SchI GAGTC 2 cut(s) 1093, 1099
ScrFI CCNGG 3 cut(s) 571, 1359, 1568
SduI GDGCHC 4 cut(s) 64, 578, 811, 1276
SfaNI GCATC 3 cut(s) 388, 808, 1396
SinI GGWCC 2 cut(s) 860, 1048
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SphI GCATGC 1 cut(s) 1330
SsiI CCGC 2 cut(s) 754, 832
SspMI CTAG 5 cut(s) 42, 195, 1013, 1200, 1310
StyD4I CCNGG 3 cut(s) 569, 1357, 1566
TaaI ACNGT 4 cut(s) 252, 1154, 1190, 1334
TaiI ACGT 2 cut(s) 1273, 1323
TaqI TCGA 4 cut(s) 360, 838, 1390, 1547
TatI WGTACW 3 cut(s) 585, 673, 1154
TauI GCSGC 2 cut(s) 757, 834
TfiI GAWTC 4 cut(s) 342, 439, 457, 473
Tru1I TTAA 6 cut(s) 516, 546, 618, 785, 1053, 1452
Tru9I TTAA 6 cut(s) 516, 546, 618, 785, 1053, 1452
TscAI CASTG 3 cut(s) 1072, 1236, 1339
TseI GCWGC 7 cut(s) 70, 73, 695, 962, 1126, 1133, 1304
TspDTI ATGAA 5 cut(s) 334, 475, 502, 623, 1518
TspRI CASTG 3 cut(s) 1072, 1236, 1339
VpaK11BI GGWCC 2 cut(s) 860, 1048
VspI ATTAAT 1 cut(s) 516
XagI CCTNNNNNAGG 1 cut(s) 45
XapI RAATTY 2 cut(s) 923, 1210
XceI RCATGY 2 cut(s) 1330, 1579
XcmI CCANNNNNNNNNTGG 2 cut(s) 652, 880
XmiI GTMKAC 1 cut(s) 1185
XmnI GAANNNNTTC 1 cut(s) 1291
XspI CTAG 5 cut(s) 42, 195, 1013, 1200, 1310
ZrmI AGTACT 2 cut(s) 587, 1156
Zsp2I ATGCAT 1 cut(s) 1378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.