AT5G65820

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012937
Physical Location & Seq
Reverse (-)
26339876 .. 26341789
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65820.1

Sequence Viewer

Length: 1914 bp
ATGCTACCGTTTACGAGAGAGATGCATAGATTCTCTCCCATAGTCGAGTTTCTTCTTAGCAAACGCCAAATTTTCTTACATTATTCTCAATTTAAGTCTCGCTTTGACTTGATTCACCGAAGTTTTCACGTTTCTAGAGCTCTCGAAGATAATTTCCGGAGAAGTAACGGGATTGGCTTAGTTTGTCTCGAGAAGAGCCACAACGATCGCACGAAAAACTCCAAGTATGATGAATTCGCGAGTGATGTCGAGAAATCATACAGAATTCTGCGGAAATTTCATTCTAGGGTTCCGAAGTTGGAACTCGCGCTTAACGAATCCGGTGTTGAACTCCGACCCGGGTTAATCGAACGGGTTTTGAATCGTTGTGGAGATGCTGGGAATCTAGGTTACAGATTCTTCGTCTGGGCGGCGAAGCAACCTCGTTATTGTCACAGTATCGAAGTGTATAAGTCAATGGTGAAGATTCTTAGTAAAATGCGTCAATTTGGAGCTGTTTGGGGTTTAATTGAAGAAATGAGGAAGGAGAATCCGCAGTTGATTGAACCGGAATTGTTTGTGGTTTTGGTTCAGAGATTTGCGTCTGCGGATATGGTGAAGAAAGCCATTGAGGTGCTCGACGAAATGCCTAAGTTCGGTTTTGAGCCAGATGAGTATGTATTCGGGTGTTTGCTAGATGCATTGTGTAAGCACGGTAGTGTTAAGGATGCTGCAAAGCTTTTTGAGGATATGAGAATGCGGTTTCCGGTGAATTTGCGGTATTTTACTTCGTTGTTGTATGGTTGGTGCAGGGTAGGGAAGATGATGGAAGCTAAATATGTTTTGGTTCAAATGAACGAAGCTGGGTTTGAGCCAGACATTGTTGATTACACTAACTTGCTAAGTGGATATGCTAATGCTGGTAAAATGGCAGATGCTTATGATCTTTTGAGAGATATGAGAAGAAGAGGCTTTGAACCGAATGCGAACTGTTACACGGTTTTGATTCAAGCATTGTGTAAGGTAGATAGAATGGAGGAGGCAATGAAGGTATTTGTTGAGATGGAGAGGTATGAATGTGAGGCTGATGTTGTGACTTACACTGCATTAGTTAGTGGGTTTTGCAAATGGGGAAAGATCGATAAATGTTATATTGTTTTAGACGATATGATAAAAAAAGGGCTCATGCCGTCTGAACTCACCTATATGCATATAATGGTGGCTCATGAGAAGAAGGAAAGTTTTGAAGAGTGTTTGGAGCTGATGGAGAAGATGAGGCAAATAGAGTATCATCCTGATATTGGCATTTACAATGTCGTCATCAGATTGGCTTGTAAGTTGGGAGAAGTAAAGGAAGCTGTTAGATTATGGAACGAAATGGAAGAAAACGGTTTGAGTCCTGGAGTAGATACGTTTGTCATAATGATCAATGGATTAGCAAGCCAAGGTTGTTTACTCGAAGCGAGTGATCACTTCAAAGAAATGGTAACTCGAGGACTGTTCTCTGTTTCTCAATATGGAACTCTGAAGTTATTGCTAAATACCGTTTTAAAAGACAAGAAGCTTGAAATGGCGAAAGATGTTTGGAGTTGTATCACAAGTAAAGGTGCTTGCGAGCTGAATGTATTATCTTGGACAATATGGATACATGCCTTGTTCTCAAAAGGCTATGAAAAGGAAGCGTGTTCTTATTGTATTGAAATGATAGAGATGGACTTCATGCCACAACCAGACACATTTGCTAAACTTATGAAGGGTTTGAAGAAATTGTATAATAGAGAATTTGCTGGAGAGATTACAGAGAAGGTGAGGAATATGGCAGCTGAGAGAGAGATGAGTTTTAAGATGTATAAGAGAAGAGGAGTGCAAGATTTGACTGAGAAAGCTAAATCTAAACAAGATAGAGAAGGGAAGAAGAAACAGAGGAGTCGATAA

Protein Analysis

637

Amino Acids

74.12

Weight (kDa)

8.52

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 189 - 230 2.3e-07 PPR repeat family
PPR_1 PF12854 213 - 245 4e-09 PPR repeat
PPR_3 PF13812 275 - 330 1.7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 282 - 314 7.2e-07 PPR repeat
PPR_2 PF13041 285 - 334 7.9e-16 PPR repeat family
PPR_long PF17177 304 - 398 3e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 309 - 353 2.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 316 - 349 7.3e-09 PPR repeat
PPR PF01535 324 - 352 1.1e-06 PPR repeat
PPR_2 PF13041 324 - 369 1e-14 PPR repeat family
PPR_3 PF13812 343 - 400 6.8e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 353 - 383 1e-07 PPR repeat
TPR_24 PF23276 354 - 452 1.4e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 356 - 395 6.4e-10 PPR repeat family
PPR PF01535 358 - 388 3e-07 PPR repeat
PPR_1 PF12854 423 - 454 3.2e-06 PPR repeat
PPR_2 PF13041 425 - 473 8.8e-15 PPR repeat family
PPR PF01535 429 - 458 1.3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65820
fragaria_vesca FvH4_1g18320
prunus_persica Prupe.6G215900_v2.0.a1
pyrus_communis pycom02g15460
rosa_chinensis RchiOBHm_Chr2g0108971
rosa_laevigata RLG00000017699
rosa_multiflora Rmu_co8481135.1_g000001 Rmu_sc0014080.1_g000003
rosa_roxburghii Rroxscaffold_2G00134670
rosa_rugosa Rorug02G0156700
rosa_samantha Rh2AG150300 Rh2AG207400 Rh2BG155900 Rh2BG218600 Rh2CG210600 Rh2DG156000 Rh2DG213600
rosa_wichuraiana Rw0G010990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 239, 308
AccIII TCCGGA 1 cut(s) 156
AciI CCGC 6 cut(s) 271, 410, 533, 587, 739, 757
AcsI RAATTY 6 cut(s) 69, 233, 264, 275, 751, 1760
AcuI CTGAAG 1 cut(s) 1526
AfiI CCNNNNNNNGG 2 cut(s) 635, 1280
AjnI CCWGG 1 cut(s) 1378
AleI CACNNNNGTG 1 cut(s) 696
Alw21I GWGCWC 2 cut(s) 142, 618
Alw26I GTCTC 2 cut(s) 102, 191
Ama87I CYCGRG 3 cut(s) 188, 338, 1470
Aor13HI TCCGGA 1 cut(s) 156
ApeKI GCWGC 2 cut(s) 710, 1799
ApoI RAATTY 6 cut(s) 69, 233, 264, 275, 751, 1760
AspLEI GCGC 1 cut(s) 310
AsuC2I CCSGG 2 cut(s) 339, 340
AsuHPI GGTGA 6 cut(s) 107, 472, 607, 760, 1171, 1798
AvaI CYCGRG 3 cut(s) 188, 338, 1470
BaeI ACNNNNGTAYC 2 cut(s) 1380, 1413
BanII GRGCYC 2 cut(s) 142, 1164
Bbv12I GWGCWC 2 cut(s) 142, 618
BbvI GCAGC 2 cut(s) 697, 1811
BccI CCATC 4 cut(s) 799, 1036, 1237, 1684
BceAI ACGGC 1 cut(s) 1153
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciT130I CCWGG 1 cut(s) 1380
BciVI GTATCC 1 cut(s) 1617
BclI TGATCA 2 cut(s) 1404, 1447
BcnI CCSGG 2 cut(s) 339, 340
BcoDI GTCTC 2 cut(s) 102, 191
BfaI CTAG 4 cut(s) 135, 285, 386, 674
BfuI GTATCC 1 cut(s) 1617
BisI GCNGC 3 cut(s) 411, 711, 1800
BlsI GCNGC 3 cut(s) 412, 712, 1801
Bme1390I CCNGG 3 cut(s) 339, 340, 1380
BmeT110I CYCGRG 3 cut(s) 188, 338, 1470
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 3 cut(s) 339, 340, 1380
BmsI GCATC 5 cut(s) 12, 364, 667, 697, 904
BpmI CTGGAG 2 cut(s) 1401, 1788
BpuMI CCSGG 2 cut(s) 339, 340
Bsa29I ATCGAT 1 cut(s) 1119
BsaJI CCNNGG 2 cut(s) 338, 1423
BsaWI WCCGGW 4 cut(s) 156, 320, 547, 745
Bsc4I CCNNNNNNNGG 2 cut(s) 635, 1280
Bse3DI GCAATG 1 cut(s) 1029
BseAI TCCGGA 1 cut(s) 156
BseBI CCWGG 1 cut(s) 1380
BseCI ATCGAT 1 cut(s) 1119
BseDI CCNNGG 2 cut(s) 338, 1423
BseGI GGATG 2 cut(s) 712, 1270
BseLI CCNNNNNNNGG 2 cut(s) 635, 1280
BseMI GCAATG 1 cut(s) 1029
BseMII CTCAG 2 cut(s) 1794, 1848
BseRI GAGGAG 2 cut(s) 1031, 1854
BseXI GCAGC 2 cut(s) 697, 1811
BseYI CCCAGC 2 cut(s) 377, 842
BsgI GTGCAG 1 cut(s) 808
Bsh1236I CGCG 2 cut(s) 239, 308
Bsh1285I CGRYCG 1 cut(s) 208
BshVI ATCGAT 1 cut(s) 1119
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 142, 618
BsiHKCI CYCGRG 3 cut(s) 188, 338, 1470
BsiSI CCGG 5 cut(s) 157, 321, 339, 548, 746
BslI CCNNNNNNNGG 2 cut(s) 635, 1280
BsmAI GTCTC 2 cut(s) 102, 191
BsmI GAATGC 2 cut(s) 741, 967
BsoBI CYCGRG 3 cut(s) 188, 338, 1470
Bsp1286I GDGCHC 3 cut(s) 142, 618, 1164
Bsp13I TCCGGA 1 cut(s) 156
Bsp143I GATC 5 cut(s) 205, 922, 1116, 1404, 1447
Bsp68I TCGCGA 1 cut(s) 239
BspACI CCGC 6 cut(s) 271, 410, 533, 587, 739, 757
BspCNI CTCAG 2 cut(s) 1795, 1849
BspDI ATCGAT 1 cut(s) 1119
BspEI TCCGGA 1 cut(s) 156
BspFNI CGCG 2 cut(s) 239, 308
BspHI TCATGA 1 cut(s) 1204
BspLI GGNNCC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 1029
BssECI CCNNGG 2 cut(s) 338, 1423
BssMI GATC 5 cut(s) 205, 922, 1116, 1404, 1447
BssT1I CCWWGG 1 cut(s) 1423
Bst2UI CCWGG 1 cut(s) 1380
Bst4CI ACNGT 8 cut(s) 9, 437, 695, 971, 979, 1370, 1479, 1525
Bst6I CTCTTC 4 cut(s) 188, 940, 1221, 1831
BstC8I GCNNGC 3 cut(s) 1420, 1591, 1595
BstDEI CTNAG 7 cut(s) 56, 178, 470, 630, 881, 1803, 1857
BstF5I GGATG 2 cut(s) 712, 1270
BstFNI CGCG 2 cut(s) 239, 308
BstHHI GCGC 1 cut(s) 310
BstKTI GATC 5 cut(s) 208, 925, 1119, 1407, 1450
BstMAI GTCTC 2 cut(s) 102, 191
BstMBI GATC 5 cut(s) 205, 922, 1116, 1404, 1447
BstMCI CGRYCG 1 cut(s) 208
BstNI CCWGG 1 cut(s) 1380
BstNSI RCATGY 1 cut(s) 1631
BstSCI CCNGG 3 cut(s) 337, 338, 1378
BstUI CGCG 2 cut(s) 239, 308
BstV1I GCAGC 2 cut(s) 697, 1811
Bsu15I ATCGAT 1 cut(s) 1119
BsuI GTATCC 1 cut(s) 1617
BsuTUI ATCGAT 1 cut(s) 1119
BtsCI GGATG 2 cut(s) 712, 1270
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 1 cut(s) 1080
BtuMI TCGCGA 1 cut(s) 239
Cac8I GCNNGC 3 cut(s) 1420, 1591, 1595
CciI TCATGA 1 cut(s) 1204
CfoI GCGC 1 cut(s) 310
Cfr9I CCCGGG 1 cut(s) 338
ClaI ATCGAT 1 cut(s) 1119
CseI GACGC 2 cut(s) 470, 570
CviAII CATG 4 cut(s) 1165, 1205, 1628, 1699
DdeI CTNAG 7 cut(s) 56, 178, 470, 630, 881, 1803, 1857
DpnI GATC 5 cut(s) 207, 924, 1118, 1406, 1449
DpnII GATC 5 cut(s) 205, 922, 1116, 1404, 1447
DraI TTTAAA 1 cut(s) 1530
Eam1104I CTCTTC 4 cut(s) 188, 940, 1221, 1831
EarI CTCTTC 4 cut(s) 188, 940, 1221, 1831
Ecl136II GAGCTC 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 1423
Eco24I GRGCYC 2 cut(s) 142, 1164
Eco53kI GAGCTC 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 1526
Eco88I CYCGRG 3 cut(s) 188, 338, 1470
EcoICRI GAGCTC 1 cut(s) 140
EcoRI GAATTC 2 cut(s) 233, 264
EcoRII CCWGG 1 cut(s) 1378
EcoT14I CCWWGG 1 cut(s) 1423
EcoT22I ATGCAT 3 cut(s) 27, 682, 1191
EcoT38I GRGCYC 2 cut(s) 142, 1164
ErhI CCWWGG 1 cut(s) 1423
FaeI CATG 4 cut(s) 1168, 1208, 1631, 1702
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FatI CATG 4 cut(s) 1164, 1204, 1627, 1698
FbaI TGATCA 2 cut(s) 1404, 1447
Fnu4HI GCNGC 3 cut(s) 411, 711, 1800
FokI GGATG 2 cut(s) 719, 1257
FriOI GRGCYC 2 cut(s) 142, 1164
Fsp4HI GCNGC 3 cut(s) 411, 711, 1800
FspBI CTAG 4 cut(s) 135, 285, 386, 674
GlaI GCGC 1 cut(s) 309
GluI GCNGC 3 cut(s) 411, 711, 1800
GsaI CCCAGC 2 cut(s) 381, 846
GsuI CTGGAG 2 cut(s) 1401, 1788
HapII CCGG 5 cut(s) 157, 321, 339, 548, 746
HgaI GACGC 2 cut(s) 470, 570
HhaI GCGC 1 cut(s) 310
Hin1II CATG 4 cut(s) 1168, 1208, 1631, 1702
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HindIII AAGCTT 2 cut(s) 716, 1541
HpaII CCGG 5 cut(s) 157, 321, 339, 548, 746
HphI GGTGA 6 cut(s) 107, 472, 607, 760, 1171, 1798
Hpy166II GTNNAC 2 cut(s) 12, 1433
Hpy188I TCNGA 6 cut(s) 294, 335, 573, 1174, 1304, 1506
Hpy188III TCNNGA 9 cut(s) 135, 143, 157, 188, 190, 238, 250, 1205, 1274
Hpy8I GTNNAC 2 cut(s) 12, 1433
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 6 cut(s) 517, 1021, 1207, 1726, 1777, 1880
HpyCH4III ACNGT 8 cut(s) 9, 437, 695, 971, 979, 1370, 1479, 1525
HpyCH4IV ACGT 2 cut(s) 129, 1391
HpyCH4V TGCA 8 cut(s) 25, 680, 713, 789, 1085, 1104, 1189, 1846
HpyF3I CTNAG 7 cut(s) 56, 178, 470, 630, 881, 1803, 1857
HpySE526I ACGT 2 cut(s) 129, 1391
Hsp92II CATG 4 cut(s) 1168, 1208, 1631, 1702
HspAI GCGC 1 cut(s) 308
Kpn2I TCCGGA 1 cut(s) 156
Ksp22I TGATCA 2 cut(s) 1404, 1447
Kzo9I GATC 5 cut(s) 205, 922, 1116, 1404, 1447
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 2 cut(s) 491, 1237
Lsp1109I GCAGC 2 cut(s) 697, 1811
LweI GCATC 5 cut(s) 12, 364, 667, 697, 904
MaeI CTAG 4 cut(s) 135, 285, 386, 674
MaeII ACGT 2 cut(s) 129, 1391
MaeIII GTNAC 6 cut(s) 164, 389, 431, 971, 1072, 1465
MalI GATC 5 cut(s) 207, 924, 1118, 1406, 1449
MboI GATC 5 cut(s) 205, 922, 1116, 1404, 1447
MhlI GDGCHC 3 cut(s) 142, 618, 1164
MlyI GAGTC 1 cut(s) 1384
MmeI TCCRAC 2 cut(s) 279, 358
Mph1103I ATGCAT 3 cut(s) 27, 682, 1191
MroI TCCGGA 1 cut(s) 156
MseI TTAA 7 cut(s) 93, 312, 344, 506, 702, 1529, 1821
MslI CAYNNNNRTG 3 cut(s) 611, 696, 1184
MspA1I CMGCKG 1 cut(s) 1802
MspI CCGG 5 cut(s) 157, 321, 339, 548, 746
MspR9I CCNGG 3 cut(s) 339, 340, 1380
Mva1269I GAATGC 2 cut(s) 741, 967
MvaI CCWGG 1 cut(s) 1380
MvnI CGCG 2 cut(s) 239, 308
NciI CCSGG 2 cut(s) 339, 340
NdeII GATC 5 cut(s) 205, 922, 1116, 1404, 1447
NlaIII CATG 4 cut(s) 1168, 1208, 1631, 1702
NlaIV GGNNCC 1 cut(s) 291
NmuCI GTSAC 2 cut(s) 431, 1072
NruI TCGCGA 1 cut(s) 239
NsiI ATGCAT 3 cut(s) 27, 682, 1191
NspI RCATGY 1 cut(s) 1631
OliI CACNNNNGTG 1 cut(s) 696
PaeR7I CTCGAG 2 cut(s) 188, 1470
PagI TCATGA 1 cut(s) 1204
PciSI GCTCTTC 1 cut(s) 188
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 2 cut(s) 741, 967
PfeI GAWTC 9 cut(s) 30, 112, 317, 361, 382, 396, 466, 529, 985
PfoI TCCNGGA 1 cut(s) 1378
PkrI GCNGC 3 cut(s) 412, 712, 1801
Ple19I CGATCG 1 cut(s) 208
PleI GAGTC 1 cut(s) 1383
PpsI GAGTC 1 cut(s) 1383
Psp124BI GAGCTC 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 1378
PspFI CCCAGC 2 cut(s) 377, 842
PspGI CCWGG 1 cut(s) 1378
PspN4I GGNNCC 1 cut(s) 291
PspXI VCTCGAGB 1 cut(s) 1470
PvuI CGATCG 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 1802
RruI TCGCGA 1 cut(s) 239
RseI CAYNNNNRTG 3 cut(s) 611, 696, 1184
SacI GAGCTC 1 cut(s) 142
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 7 cut(s) 93, 312, 344, 506, 702, 1529, 1821
SatI GCNGC 3 cut(s) 411, 711, 1800
Sau3AI GATC 5 cut(s) 205, 922, 1116, 1404, 1447
SchI GAGTC 1 cut(s) 1384
ScrFI CCNGG 3 cut(s) 339, 340, 1380
SduI GDGCHC 3 cut(s) 142, 618, 1164
SfaNI GCATC 5 cut(s) 12, 364, 667, 697, 904
Sfr274I CTCGAG 2 cut(s) 188, 1470
SlaI CTCGAG 2 cut(s) 188, 1470
SmaI CCCGGG 1 cut(s) 340
SmiMI CAYNNNNRTG 3 cut(s) 611, 696, 1184
SmlI CTYRAG 2 cut(s) 188, 1470
SmoI CTYRAG 2 cut(s) 188, 1470
SsiI CCGC 6 cut(s) 271, 410, 533, 587, 739, 757
SspMI CTAG 4 cut(s) 135, 285, 386, 674
SstI GAGCTC 1 cut(s) 142
StyD4I CCNGG 3 cut(s) 337, 338, 1378
StyI CCWWGG 1 cut(s) 1423
TaaI ACNGT 8 cut(s) 9, 437, 695, 971, 979, 1370, 1479, 1525
TaiI ACGT 2 cut(s) 132, 1394
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 9 cut(s) 30, 112, 317, 361, 382, 396, 466, 529, 985
Tru1I TTAA 7 cut(s) 93, 312, 344, 506, 702, 1529, 1821
Tru9I TTAA 7 cut(s) 93, 312, 344, 506, 702, 1529, 1821
TscAI CASTG 1 cut(s) 1087
TseFI GTSAC 2 cut(s) 431, 1072
TseI GCWGC 2 cut(s) 710, 1799
Tsp45I GTSAC 2 cut(s) 431, 1072
TspDTI ATGAA 8 cut(s) 246, 269, 848, 1040, 1068, 1665, 1687, 1745
TspMI CCCGGG 1 cut(s) 338
TspRI CASTG 1 cut(s) 1087
XapI RAATTY 6 cut(s) 69, 233, 264, 275, 751, 1760
XbaI TCTAGA 1 cut(s) 134
XceI RCATGY 1 cut(s) 1631
XhoI CTCGAG 2 cut(s) 188, 1470
XmaI CCCGGG 1 cut(s) 338
XspI CTAG 4 cut(s) 135, 285, 386, 674
Zsp2I ATGCAT 3 cut(s) 27, 682, 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.