pycom02g15460

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012937
Physical Location & Seq
Forward (+)
12281793 .. 12283715
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15460.1

Sequence Viewer

Length: 1923 bp
ATGCAGAGGTTGTATCAGCAAACCAAATCTTTCTGCACCAAACACCATCATCTCTCGCATTCCTTCAAACCAAACGCCCCCTCAAACCTTTTCCACACCAATCACAAACCCACCAAGCAAACCCACAACCCCCATGCCTTCACCGAGAGAAGATCCGGCCTCGGGCTGGCCGGCTTCGACACCGACGCGGATACATCCCCAGACGACCCGATCCACTGCGAGTTCTCCGCCGACGTCGAGAAGATATACAGAATACTACGGAAATTCCACACCAGGGTCCCGAAATTGGAGCTCGCTCTGCACCAATGCGGCGTCGTTTTGAGGTCGGGGTTAACGGAGCGGGTGCTGAACCGGTGCGGCGACGCCGGGAACCTCGGGTATAGGTTCTTCGTGTGGGCTGCGCAGCAGCCGAATTACAGACCCAGTTACGAGGTGTATAAGGCCATGATTAAAAGCTTGAGCAAAATGCGCCAATTCGGCGCCGTTTGGGCGCTGCTTGAGGAGATGAGGAGGGAGAATCCGCAGCTGATTACCCAGGAAGTTTTTGTGGTTCTGATGCGGCGGTTCGCGGCGGCGAGGATGGTGAAGAAAGCAGTTGAGGTGCTCGACGAAATGCCGAAGTATGGGTGCGAACCCGATGAGTATGTTTTCGGGTGTTTGTTGGATGCCTTGTGTAAAAATGGTAGTGTTAAAGATGCAGCTTCATTGTTTGAGGATATGCGTGTGAGGTTTACTCCGAGTATTAAGCATTTTACGTCGTTGTTGTATGGTTGGTGTCGAGAAGGGAAGCTCATGGAGGCGAAATTCGTGTTGGTGCAGATGAGGGAAGCCGGTTTTGAGCCTGACATTGTGGTGTATAACACATTGCTGAGCGGGTACTCTCAGGCTGGGAAGATGGTTGATGCCAATGAGCTCTTGAAGGAGATGAGGAGGAAGGGTTGTGAGCCCAATGCAGCTTCGTACACGACTGTGATTCAGGCGCTTTGCAGTCGAGAAAAGATGGAGGAGGCAATGAGGGTGTTTGTGGAGATGCAGAGGAGTGGTTGTGAGGCTGATGTTGTGACTTACACTACTTTGATTAGTGGGTTTTGTAAGTGGGGAAAGATTGAGAGGAGTTACGAGATTTTGGATGGTATGATACAGAAAGGGTTTTTGCCGAATCAGATGACTTACATGTATATTATGTTAGCTCATGAGAAGAAGGAAGAGCTGGAGGAGTGTGTGGAATTGATGGAGGAGATGAGAAAGATCGGTTGTAGTCCTGATCTTGGTATTTACAACACGGTGATCCGGTTAGCGTGCAAATTGGGGGAGGTGAAAGAAGGTGTTCGACTTTGGAACGAAATGGAAGCAGCCGGGTTTAGTCCCGGGCTTGACACCTTTGTCATTATGATTCATGGGTTTCTTGGGCATGGCTGTTTGATTGATGCTTGTGATTATTTTAAAGAAATGGTTGGCAGAGGTCTTCTGTCTGGCCCACAATATGGTACCTTGAAGGAGCTGATGAATGCTTTGTTGAGAGACGAGAAGCTAGAAATGGCAAAAGATGTTTGGAGTTGCATTGTGACCAAGGGATGTGATCTCAATGTGTTTGCATGGACAATTTGGATTCATGCGCTGTTTTCAAAGGGGCATGTGAAGGAGGCTTGTTCGTACTGTTTGGACATGATGGAGGCGGATGTAATGCCGCAGCCAGATACTTTTGCGAAGCTAATGCGCGGTTTAAGGAAACTATATAACCGACAGATAGCAGCTGAGATCACAGAGAAGGTGAGGAAGATGGCTGCAGATAGACAAATCACTTTCAAGATGTATAAAAGGCGAGGAGAGCGGGACTTGAAGGAAAAGGTAAAGGAGAAAAATGATGGGAGGAAACGGAGGGCCCGAAGAAGAAGTTGGCCTAGTAAATCTAAATCTTTGTAG

Protein Analysis

641

Amino Acids

73.63

Weight (kDa)

8.91

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 184 - 226 5.6e-09 PPR repeat family
PPR_1 PF12854 209 - 241 1.6e-09 PPR repeat
TPR_24 PF23276 249 - 343 1.8e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 270 - 383 1.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 271 - 328 8.9e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 278 - 310 5.2e-09 PPR repeat
PPR_2 PF13041 281 - 329 2.4e-18 PPR repeat family
PPR PF01535 284 - 314 4e-09 PPR repeat
PPR_1 PF12854 312 - 345 1.5e-09 PPR repeat
PPR_2 PF13041 316 - 365 1.4e-18 PPR repeat family
PPR PF01535 320 - 349 1.1e-07 PPR repeat
TPR_24 PF23276 337 - 448 2.1e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 339 - 396 9.3e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 348 - 379 8.2e-11 PPR repeat
PPR_2 PF13041 352 - 394 1.1e-09 PPR repeat family
PPR PF01535 354 - 384 1.7e-07 PPR repeat
PPR_1 PF12854 418 - 450 4.3e-07 PPR repeat
PPR_2 PF13041 421 - 468 6.9e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65820
fragaria_vesca FvH4_1g18320
prunus_persica Prupe.6G215900_v2.0.a1
pyrus_communis pycom02g15460
rosa_chinensis RchiOBHm_Chr2g0108971
rosa_laevigata RLG00000017699
rosa_multiflora Rmu_co8481135.1_g000001 Rmu_sc0014080.1_g000003
rosa_roxburghii Rroxscaffold_2G00134670
rosa_rugosa Rorug02G0156700
rosa_samantha Rh2AG150300 Rh2AG207400 Rh2BG155900 Rh2BG218600 Rh2CG210600 Rh2DG156000 Rh2DG213600
rosa_wichuraiana Rw0G010990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1382
AatII GACGTC 1 cut(s) 237
Acc16I TGCGCA 1 cut(s) 402
Acc65I GGTACC 1 cut(s) 1487
AccB1I GGYRCC 2 cut(s) 479, 1487
AccB7I CCANNNNNTGG 1 cut(s) 1484
AccBSI CCGCTC 3 cut(s) 340, 873, 1831
AccII CGCG 3 cut(s) 188, 569, 1719
AclWI GGATC 3 cut(s) 147, 205, 1282
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 2 cut(s) 263, 803
AcyI GRCGYC 4 cut(s) 234, 312, 363, 480
AfaI GTAC 4 cut(s) 878, 962, 1489, 1655
AfiI CCNNNNNNNGG 7 cut(s) 162, 166, 274, 286, 623, 1268, 1484
AflIII ACRYGT 1 cut(s) 1173
AgeI ACCGGT 1 cut(s) 351
AgsI TTSAA 6 cut(s) 67, 919, 1495, 1626, 1807, 1840
AjnI CCWGG 2 cut(s) 272, 534
AjuI GAANNNNNNNTTGG 4 cut(s) 794, 826, 1879, 1911
AleI CACNNNNGTG 1 cut(s) 968
Alw21I GWGCWC 3 cut(s) 294, 606, 915
Alw26I GTCTC 1 cut(s) 1515
AlwI GGATC 3 cut(s) 147, 205, 1282
Ama87I CYCGRG 3 cut(s) 161, 374, 1367
AoxI GGCC 6 cut(s) 157, 168, 441, 1474, 1881, 1898
ApaI GGGCCC 1 cut(s) 1885
ApoI RAATTY 2 cut(s) 263, 803
AsiGI ACCGGT 1 cut(s) 351
Asp700I GAANNNNTTC 1 cut(s) 1326
Asp718I GGTACC 1 cut(s) 1487
AspLEI GCGC 7 cut(s) 403, 471, 482, 493, 982, 1618, 1719
AspS9I GGNCC 4 cut(s) 277, 1475, 1881, 1882
AsuC2I CCSGG 4 cut(s) 367, 1357, 1368, 1369
AsuHPI GGTGA 5 cut(s) 133, 595, 1297, 1327, 1783
AvaI CYCGRG 3 cut(s) 161, 374, 1367
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 1 cut(s) 1885
BanI GGYRCC 2 cut(s) 479, 1487
BanII GRGCYC 4 cut(s) 294, 915, 948, 1885
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
BbsI GAAGAC 1 cut(s) 1457
Bbv12I GWGCWC 3 cut(s) 294, 606, 915
BccI CCATC 9 cut(s) 54, 574, 889, 994, 1124, 1225, 1663, 1774, 1859
BceAI ACGGC 1 cut(s) 467
BcgI CGANNNNNNTGC 2 cut(s) 1696, 1730
BciT130I CCWGG 2 cut(s) 274, 536
BciVI GTATCC 1 cut(s) 184
BcnI CCSGG 4 cut(s) 367, 1357, 1368, 1369
BcoDI GTCTC 1 cut(s) 1515
BfaI CTAG 2 cut(s) 1532, 1902
BfmI CTRYAG 1 cut(s) 1785
BfoI RGCGCY 3 cut(s) 483, 494, 983
BfuI GTATCC 1 cut(s) 184
BglI GCCNNNNNGGC 2 cut(s) 477, 488
BlpI GCTNAGC 1 cut(s) 869
Bme1390I CCNGG 6 cut(s) 274, 367, 536, 1357, 1368, 1369
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 3 cut(s) 161, 374, 1367
BmgT120I GGNCC 4 cut(s) 277, 1475, 1881, 1882
BmiI GGNNCC 6 cut(s) 278, 279, 371, 481, 1489, 1883
BmrFI CCNGG 6 cut(s) 274, 367, 536, 1357, 1368, 1369
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 6 cut(s) 546, 655, 685, 892, 1020, 1417
BmuI ACTGGG 1 cut(s) 417
BpiI GAAGAC 1 cut(s) 1457
BplI GAGNNNNNCTC 2 cut(s) 718, 750
BpmI CTGGAG 1 cut(s) 1232
Bpu1102I GCTNAGC 1 cut(s) 869
BpuEI CTTGAG 2 cut(s) 478, 518
BpuMI CCSGG 4 cut(s) 367, 1357, 1368, 1369
BsaHI GRCGYC 4 cut(s) 234, 312, 363, 480
BsaJI CCNNGG 6 cut(s) 160, 273, 373, 534, 1367, 1569
BsaWI WCCGGW 2 cut(s) 351, 1290
BsaXI ACNNNNNCTCC 4 cut(s) 922, 952, 1030, 1060
Bsc4I CCNNNNNNNGG 7 cut(s) 162, 166, 274, 286, 623, 1268, 1484
Bse118I RCCGGY 3 cut(s) 170, 351, 830
Bse1I ACTGG 1 cut(s) 423
Bse3DI GCAATG 2 cut(s) 863, 1017
BseBI CCWGG 2 cut(s) 274, 536
BseDI CCNNGG 6 cut(s) 160, 273, 373, 534, 1367, 1569
BseGI GGATG 6 cut(s) 194, 585, 670, 1135, 1580, 1684
BseLI CCNNNNNNNGG 7 cut(s) 162, 166, 274, 286, 623, 1268, 1484
BseMI GCAATG 2 cut(s) 863, 1017
BseMII CTCAG 3 cut(s) 860, 896, 1746
BseNI ACTGG 1 cut(s) 423
BseRI GAGGAG 9 cut(s) 515, 523, 943, 1019, 1051, 1126, 1229, 1250, 1839
BseSI GKGCMC 1 cut(s) 1885
BseYI CCCAGC 1 cut(s) 887
BsgI GTGCAG 3 cut(s) 19, 284, 836
Bsh1236I CGCG 3 cut(s) 188, 569, 1719
BshFI GGCC 6 cut(s) 159, 170, 443, 1476, 1883, 1900
BshNI GGYRCC 2 cut(s) 479, 1487
BshTI ACCGGT 1 cut(s) 351
BsiHKAI GWGCWC 3 cut(s) 294, 606, 915
BsiHKCI CYCGRG 3 cut(s) 161, 374, 1367
BsiSI CCGG 8 cut(s) 156, 171, 352, 366, 831, 1291, 1356, 1368
BslFI GGGAC 3 cut(s) 263, 1350, 1847
BslI CCNNNNNNNGG 7 cut(s) 162, 166, 274, 286, 623, 1268, 1484
BsmAI GTCTC 1 cut(s) 1515
BsmBI CGTCTC 1 cut(s) 1515
BsmFI GGGAC 3 cut(s) 263, 1350, 1847
BsmI GAATGC 2 cut(s) 58, 1513
BsnI GGCC 6 cut(s) 159, 170, 443, 1476, 1883, 1900
BsoBI CYCGRG 3 cut(s) 161, 374, 1367
Bsp120I GGGCCC 1 cut(s) 1881
Bsp1286I GDGCHC 5 cut(s) 294, 606, 915, 948, 1885
Bsp143I GATC 7 cut(s) 152, 210, 1248, 1264, 1287, 1579, 1758
Bsp1720I GCTNAGC 1 cut(s) 869
BspANI GGCC 6 cut(s) 159, 170, 443, 1476, 1883, 1900
BspCNI CTCAG 3 cut(s) 861, 895, 1747
BspFNI CGCG 3 cut(s) 188, 569, 1719
BspHI TCATGA 1 cut(s) 1192
BspLI GGNNCC 6 cut(s) 278, 279, 371, 481, 1489, 1883
BspMAI CTGCAG 1 cut(s) 1789
BspPI GGATC 3 cut(s) 147, 205, 1282
BspQI GCTCTTC 1 cut(s) 1200
BspT107I GGYRCC 2 cut(s) 479, 1487
BsrBI CCGCTC 3 cut(s) 340, 873, 1831
BsrDI GCAATG 2 cut(s) 863, 1017
BsrFI RCCGGY 3 cut(s) 170, 351, 830
BsrI ACTGG 1 cut(s) 423
BssAI RCCGGY 3 cut(s) 170, 351, 830
BssECI CCNNGG 6 cut(s) 160, 273, 373, 534, 1367, 1569
BssMI GATC 7 cut(s) 152, 210, 1248, 1264, 1287, 1579, 1758
BssNI GRCGYC 4 cut(s) 234, 312, 363, 480
BssT1I CCWWGG 1 cut(s) 1569
Bst2UI CCWGG 2 cut(s) 274, 536
Bst4CI ACNGT 3 cut(s) 970, 1285, 1658
Bst6I CTCTTC 1 cut(s) 1200
BstACI GRCGYC 4 cut(s) 234, 312, 363, 480
BstC8I GCNNGC 4 cut(s) 168, 172, 294, 1300
BstDEI CTNAG 3 cut(s) 869, 882, 1755
BstF5I GGATG 6 cut(s) 194, 585, 670, 1135, 1580, 1684
BstFNI CGCG 3 cut(s) 188, 569, 1719
BstH2I RGCGCY 3 cut(s) 483, 494, 983
BstHHI GCGC 7 cut(s) 403, 471, 482, 493, 982, 1618, 1719
BstKTI GATC 7 cut(s) 155, 213, 1251, 1267, 1290, 1582, 1761
BstMAI GTCTC 1 cut(s) 1515
BstMBI GATC 7 cut(s) 152, 210, 1248, 1264, 1287, 1579, 1758
BstMWI GCNNNNNNNGC 5 cut(s) 298, 468, 477, 488, 1828
BstNI CCWGG 2 cut(s) 274, 536
BstNSI RCATGY 2 cut(s) 1177, 1637
BstSCI CCNGG 6 cut(s) 272, 365, 534, 1355, 1366, 1367
BstSFI CTRYAG 1 cut(s) 1785
BstSLI GKGCMC 1 cut(s) 1885
BstUI CGCG 3 cut(s) 188, 569, 1719
BstV2I GAAGAC 1 cut(s) 1457
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 6 cut(s) 159, 170, 443, 1476, 1883, 1900
BtsCI GGATG 6 cut(s) 194, 585, 670, 1135, 1580, 1684
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 214
Cac8I GCNNGC 4 cut(s) 168, 172, 294, 1300
CciI TCATGA 1 cut(s) 1192
CfoI GCGC 7 cut(s) 403, 471, 482, 493, 982, 1618, 1719
Cfr10I RCCGGY 3 cut(s) 170, 351, 830
Cfr13I GGNCC 4 cut(s) 277, 1475, 1881, 1882
Cfr9I CCCGGG 1 cut(s) 1367
CseI GACGC 3 cut(s) 194, 301, 371
Csp6I GTAC 4 cut(s) 877, 961, 1488, 1654
CspAI ACCGGT 1 cut(s) 351
CviQI GTAC 4 cut(s) 877, 961, 1488, 1654
DdeI CTNAG 3 cut(s) 869, 882, 1755
DinI GGCGCC 1 cut(s) 481
DpnI GATC 7 cut(s) 154, 212, 1250, 1266, 1289, 1581, 1760
DpnII GATC 7 cut(s) 152, 210, 1248, 1264, 1287, 1579, 1758
DraI TTTAAA 1 cut(s) 1444
DrdI GACNNNNNNGTC 1 cut(s) 1382
DseDI GACNNNNNNGTC 1 cut(s) 1382
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 1200
EarI CTCTTC 1 cut(s) 1200
EciI GGCGGA 2 cut(s) 217, 1691
Ecl136II GAGCTC 2 cut(s) 292, 913
Eco130I CCWWGG 1 cut(s) 1569
Eco24I GRGCYC 4 cut(s) 294, 915, 948, 1885
Eco47I GGWCC 1 cut(s) 277
Eco53kI GAGCTC 2 cut(s) 292, 913
Eco88I CYCGRG 3 cut(s) 161, 374, 1367
EcoICRI GAGCTC 2 cut(s) 292, 913
EcoO109I RGGNCCY 2 cut(s) 277, 1881
EcoRII CCWGG 2 cut(s) 272, 534
EcoT14I CCWWGG 1 cut(s) 1569
EcoT38I GRGCYC 4 cut(s) 294, 915, 948, 1885
EgeI GGCGCC 1 cut(s) 481
EheI GGCGCC 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 1569
Esp3I CGTCTC 1 cut(s) 1515
FaqI GGGAC 3 cut(s) 263, 1350, 1847
FauI CCCGC 3 cut(s) 333, 866, 1824
FokI GGATG 6 cut(s) 181, 592, 677, 1142, 1587, 1691
FriOI GRGCYC 4 cut(s) 294, 915, 948, 1885
FspBI CTAG 2 cut(s) 1532, 1902
FspI TGCGCA 1 cut(s) 402
GlaI GCGC 7 cut(s) 402, 470, 481, 492, 981, 1617, 1718
GsaI CCCAGC 1 cut(s) 891
GsuI CTGGAG 1 cut(s) 1232
HaeII RGCGCY 3 cut(s) 483, 494, 983
HaeIII GGCC 6 cut(s) 159, 170, 443, 1476, 1883, 1900
HapII CCGG 8 cut(s) 156, 171, 352, 366, 831, 1291, 1356, 1368
HgaI GACGC 3 cut(s) 194, 301, 371
HhaI GCGC 7 cut(s) 403, 471, 482, 493, 982, 1618, 1719
Hin1I GRCGYC 4 cut(s) 234, 312, 363, 480
Hin6I GCGC 7 cut(s) 401, 469, 480, 491, 980, 1616, 1717
HinP1I GCGC 7 cut(s) 401, 469, 480, 491, 980, 1616, 1717
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 5 cut(s) 517, 973, 1159, 1393, 1609
HpaI GTTAAC 1 cut(s) 333
HpaII CCGG 8 cut(s) 156, 171, 352, 366, 831, 1291, 1356, 1368
HphI GGTGA 5 cut(s) 133, 595, 1297, 1327, 1783
Hpy166II GTNNAC 3 cut(s) 333, 732, 963
Hpy188I TCNGA 3 cut(s) 555, 738, 1164
Hpy188III TCNNGA 8 cut(s) 238, 280, 779, 916, 992, 1193, 1262, 1807
Hpy8I GTNNAC 3 cut(s) 333, 732, 963
Hpy99I CGWCG 7 cut(s) 188, 236, 239, 317, 365, 611, 760
HpyCH4III ACNGT 3 cut(s) 970, 1285, 1658
HpyCH4IV ACGT 2 cut(s) 234, 755
HpyF10VI GCNNNNNNNGC 5 cut(s) 298, 468, 477, 488, 1828
HpyF3I CTNAG 3 cut(s) 869, 882, 1755
HpySE526I ACGT 2 cut(s) 234, 755
Hsp92I GRCGYC 4 cut(s) 234, 312, 363, 480
HspAI GCGC 7 cut(s) 401, 469, 480, 491, 980, 1616, 1717
KasI GGCGCC 1 cut(s) 479
KflI GGGWCCC 1 cut(s) 277
KpnI GGTACC 1 cut(s) 1491
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
KspAI GTTAAC 1 cut(s) 333
Kzo9I GATC 7 cut(s) 152, 210, 1248, 1264, 1287, 1579, 1758
LguI GCTCTTC 1 cut(s) 1200
LmnI GCTCC 3 cut(s) 289, 337, 1498
LweI GCATC 6 cut(s) 546, 655, 685, 892, 1020, 1417
MaeI CTAG 2 cut(s) 1532, 1902
MaeII ACGT 2 cut(s) 234, 755
MaeIII GTNAC 4 cut(s) 425, 1060, 1115, 1564
MalI GATC 7 cut(s) 154, 212, 1250, 1266, 1289, 1581, 1760
MbiI CCGCTC 3 cut(s) 340, 873, 1831
MboI GATC 7 cut(s) 152, 210, 1248, 1264, 1287, 1579, 1758
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 5 cut(s) 294, 606, 915, 948, 1885
MluCI AATT 8 cut(s) 263, 284, 412, 473, 803, 1226, 1304, 1602
Mly113I GGCGCC 1 cut(s) 480
MmeI TCCRAC 1 cut(s) 642
MroNI GCCGGC 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 1326
MseI TTAA 6 cut(s) 332, 450, 690, 744, 1443, 1724
MslI CAYNNNNRTG 2 cut(s) 851, 968
MspA1I CMGCKG 2 cut(s) 526, 1754
MspI CCGG 8 cut(s) 156, 171, 352, 366, 831, 1291, 1356, 1368
MspR9I CCNGG 6 cut(s) 274, 367, 536, 1357, 1368, 1369
Mva1269I GAATGC 2 cut(s) 58, 1513
MvaI CCWGG 2 cut(s) 274, 536
MvnI CGCG 3 cut(s) 188, 569, 1719
MwoI GCNNNNNNNGC 5 cut(s) 298, 468, 477, 488, 1828
NaeI GCCGGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 480
NciI CCSGG 4 cut(s) 367, 1357, 1368, 1369
NdeII GATC 7 cut(s) 152, 210, 1248, 1264, 1287, 1579, 1758
NgoMIV GCCGGC 1 cut(s) 170
NlaIV GGNNCC 6 cut(s) 278, 279, 371, 481, 1489, 1883
NmuCI GTSAC 2 cut(s) 1060, 1564
NsbI TGCGCA 1 cut(s) 402
NspI RCATGY 2 cut(s) 1177, 1637
OliI CACNNNNGTG 1 cut(s) 968
PagI TCATGA 1 cut(s) 1192
PciI ACATGT 1 cut(s) 1173
PciSI GCTCTTC 1 cut(s) 1200
PcsI WCGNNNNNNNCGW 1 cut(s) 1882
PctI GAATGC 2 cut(s) 58, 1513
PdiI GCCGGC 1 cut(s) 172
PdmI GAANNNNTTC 1 cut(s) 1326
PfeI GAWTC 5 cut(s) 517, 973, 1159, 1393, 1609
PflMI CCANNNNNTGG 1 cut(s) 1484
PinAI ACCGGT 1 cut(s) 351
PluTI GGCGCC 1 cut(s) 483
PpuMI RGGWCCY 1 cut(s) 277
PscI ACATGT 1 cut(s) 1173
Psp124BI GAGCTC 2 cut(s) 294, 915
Psp5II RGGWCCY 1 cut(s) 277
Psp6I CCWGG 2 cut(s) 272, 534
PspFI CCCAGC 1 cut(s) 887
PspGI CCWGG 2 cut(s) 272, 534
PspN4I GGNNCC 6 cut(s) 278, 279, 371, 481, 1489, 1883
PspOMI GGGCCC 1 cut(s) 1881
PspPI GGNCC 4 cut(s) 277, 1475, 1881, 1882
PspPPI RGGWCCY 1 cut(s) 277
PstI CTGCAG 1 cut(s) 1789
PsuI RGATCY 1 cut(s) 152
PvuII CAGCTG 2 cut(s) 526, 1754
RsaI GTAC 4 cut(s) 878, 962, 1489, 1655
RsaNI GTAC 4 cut(s) 877, 961, 1488, 1654
RseI CAYNNNNRTG 2 cut(s) 851, 968
SacI GAGCTC 2 cut(s) 294, 915
SapI GCTCTTC 1 cut(s) 1200
SaqAI TTAA 6 cut(s) 332, 450, 690, 744, 1443, 1724
Sau3AI GATC 7 cut(s) 152, 210, 1248, 1264, 1287, 1579, 1758
Sau96I GGNCC 4 cut(s) 277, 1475, 1881, 1882
ScrFI CCNGG 6 cut(s) 274, 367, 536, 1357, 1368, 1369
SduI GDGCHC 5 cut(s) 294, 606, 915, 948, 1885
SfaNI GCATC 6 cut(s) 546, 655, 685, 892, 1020, 1417
SfcI CTRYAG 1 cut(s) 1785
SfoI GGCGCC 1 cut(s) 481
SinI GGWCC 1 cut(s) 277
SmaI CCCGGG 1 cut(s) 1369
SmiMI CAYNNNNRTG 2 cut(s) 851, 968
SmlI CTYRAG 2 cut(s) 457, 497
SmoI CTYRAG 2 cut(s) 457, 497
Sse9I AATT 8 cut(s) 263, 284, 412, 473, 803, 1226, 1304, 1602
SspDI GGCGCC 1 cut(s) 479
SspMI CTAG 2 cut(s) 1532, 1902
SstI GAGCTC 2 cut(s) 294, 915
StyD4I CCNGG 6 cut(s) 272, 365, 534, 1355, 1366, 1367
StyI CCWWGG 1 cut(s) 1569
TaaI ACNGT 3 cut(s) 970, 1285, 1658
TaiI ACGT 2 cut(s) 237, 758
TaqI TCGA 6 cut(s) 177, 237, 606, 778, 991, 1330
TasI AATT 8 cut(s) 263, 284, 412, 473, 803, 1226, 1304, 1602
TauI GCSGC 6 cut(s) 312, 360, 562, 572, 575, 1690
TfiI GAWTC 5 cut(s) 517, 973, 1159, 1393, 1609
Tru1I TTAA 6 cut(s) 332, 450, 690, 744, 1443, 1724
Tru9I TTAA 6 cut(s) 332, 450, 690, 744, 1443, 1724
TscAI CASTG 1 cut(s) 221
TseFI GTSAC 2 cut(s) 1060, 1564
Tsp45I GTSAC 2 cut(s) 1060, 1564
TspDTI ATGAA 4 cut(s) 693, 1385, 1520, 1601
TspGWI ACGGA 3 cut(s) 274, 350, 1891
TspMI CCCGGG 1 cut(s) 1367
TspRI CASTG 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 1484
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 2 cut(s) 263, 803
XceI RCATGY 2 cut(s) 1177, 1637
XmaI CCCGGG 1 cut(s) 1367
XmnI GAANNNNTTC 1 cut(s) 1326
XspI CTAG 2 cut(s) 1532, 1902
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.