Rh2DG156000

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012937
Physical Location & Seq
Reverse (-)
13035243 .. 13037101
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG156000.1

Sequence Viewer

Length: 1551 bp
ATGTATTATTATGAGTTATACCTAAAAATGCAGCCCAATTACAGACCCAGCTACGATGTGTACAAAGCCATGATCAAGTCCCTAAGCAAAATGCGGCAATTCGGCGCCGTTTGGGCTCTGCTTGAGGAGATAAGGAGAGAAAATCAGGAGCTAATTACTGTGGAAATGTTTGTGGTTTTGATGCGGCGTTTCGCTTCGGCGAGGATGGTGAAGAAAGCAGTGGAGGTGCTCGATGAAATGCCCAAGTACGGGTGCGAGGCGGATGAGTATGCTTTTGGGTGCTTGCTTGATGCTCTGTGTAAAAATGGGAGTGTGAAAGAAGCGGCGAGTTTGTTTGAGGATATGAGAGTTAGGTTCAAGCCTAGTATTAGGCATTTTACTTCTTTGTTGTATGGGTGGTGTAGAGCAGGGAAGCTTATGGAGGCGAAACATGTGTTGGTGCAGATGAGGGAGGGAGGGTTCGAGCCGGATATTGTGGTGTATAACAATTTGCTTGGTGGGTATGCTCAGGCTGGGAAGATGGCTGATGCGTATGAGCTGTTGAAGGAGATGAGGAGGAAGGGTTGTGAGCCCAATGCGGCTTCGTATACGACTGTGATCCAGGCGCTTTGCGGGCAGGAGAAGATGGAGGAGGCGATGAGGGTGTTTGTGGAGATGGAGAGGAGTGGTTGTGAGGCTGATGTTGTTACTTATACTACTTTGATTGGTGGGTTTTGTAAGTGGGGGAAGATTGTGAGGAGTTACGAGATTTTGGAGAGTATGGTACAGAGAGGGTTTACGCCCAGTCAGATGACTTACTTGCAGATTATGTTGGCTCATGAAAAGAAGGAAGACTTGGAGGAGTGTGTGGAATTAATGGGGGAGATGAGGAAGATTGGTTGCATGCCTGATCTTGGTATTTACAACACGGTGATTCGGTTGGCTTGCAAATTGGGGGAGGTGAAAGAAGGCGTTCGGCTTTGGAATGAAATGGAAGAAGCTGGGTTTTCTCCGGGGCTTGACACCTTTGTCATTATGATTCATGGGTTTCTCGGGCAAGGTTGTTTGATCGAAGCTTGTGATTACTTCAAAGAAATGGTTGGCAGAGGCCTTCTATCTGGGCCACAATATGGTACCTTGAAGGAGTTAATGAATGCTTTGTTGAGAGATGAGAAGCTAGAAATGGCTAAAGATGTTTGGAGTTGCATTGTGACCAAAGGGTGTGAGCTTAATGTGTATGCATGGACAATTTGGATTCATGCACTGTTTTCAAAGGGGCATGTGAAAGAGGCTTGCTCATACTGCTTGGACATGATGGAGGCAGATGTAATGCCACAGCCGGATACTTTTGCGAAGCTCATGCGTGGTTTAAAGAAACTTTATAACAGACAGATTGCTGCTGAGATCACAGAGAAGGTGAGGCAGATGGCTGCAGATAGAAAGATCACTTTCAAGATGTATAAAAGACGCGGAGAGAGGGACTTGAACGAAAAAGTTAAGGAGAAGAAGGATGGGGGAAGGAAACGGAGGGCTCGAAAACGGCAATGGCCTAGTAAAGCTAAAGCCTTATAG

Protein Analysis

516

Amino Acids

59.44

Weight (kDa)

8.7

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 59 - 101 3e-08 PPR repeat family
PPR_long PF17177 69 - 186 1e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 84 - 116 4.8e-08 PPR repeat
PPR_3 PF13812 111 - 166 4.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 119 - 150 6.1e-06 PPR repeat
PPR_3 PF13812 146 - 203 4e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 153 - 185 1.5e-08 PPR repeat
PPR_2 PF13041 156 - 204 2.7e-18 PPR repeat family
PPR PF01535 159 - 189 2.5e-08 PPR repeat
PPR_long PF17177 174 - 266 4.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 187 - 220 6e-09 PPR repeat
PPR PF01535 195 - 224 7.3e-07 PPR repeat
PPR_2 PF13041 195 - 240 5.9e-17 PPR repeat family
TPR_24 PF23276 212 - 323 9.6e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 214 - 270 7.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 223 - 255 1.5e-09 PPR repeat
PPR_2 PF13041 227 - 267 5.1e-09 PPR repeat family
PPR PF01535 229 - 259 1.8e-06 PPR repeat
PPR_1 PF12854 293 - 325 5.2e-07 PPR repeat
PPR PF01535 300 - 329 8.6e-06 PPR repeat
PPR_2 PF13041 300 - 343 4.9e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65820
fragaria_vesca FvH4_1g18320
prunus_persica Prupe.6G215900_v2.0.a1
pyrus_communis pycom02g15460
rosa_chinensis RchiOBHm_Chr2g0108971
rosa_laevigata RLG00000017699
rosa_multiflora Rmu_co8481135.1_g000001 Rmu_sc0014080.1_g000003
rosa_roxburghii Rroxscaffold_2G00134670
rosa_rugosa Rorug02G0156700
rosa_samantha Rh2AG150300 Rh2AG207400 Rh2BG155900 Rh2BG218600 Rh2CG210600 Rh2DG156000 Rh2DG213600
rosa_wichuraiana Rw0G010990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1362
AasI GACNNNNNNGTC 1 cut(s) 1007
Acc65I GGTACC 1 cut(s) 1112
AccB1I GGYRCC 2 cut(s) 104, 1112
AccB7I CCANNNNNTGG 1 cut(s) 1109
AccI GTMKAC 1 cut(s) 587
AccII CGCG 1 cut(s) 1449
AciI CCGC 7 cut(s) 94, 184, 260, 323, 578, 612, 1449
AclWI GGATC 1 cut(s) 592
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 4 cut(s) 62, 248, 765, 1114
AfiI CCNNNNNNNGG 4 cut(s) 248, 249, 893, 1109
AflIII ACRYGT 1 cut(s) 430
AgsI TTSAA 7 cut(s) 358, 544, 1069, 1120, 1251, 1432, 1465
AjnI CCWGG 1 cut(s) 600
AjuI GAANNNNNNNTTGG 4 cut(s) 419, 451, 818, 850
AloI GAACNNNNNNTCC 2 cut(s) 443, 475
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 1 cut(s) 592
Ama87I CYCGRG 1 cut(s) 1031
AoxI GGCC 3 cut(s) 1087, 1100, 1526
ApeKI GCWGC 3 cut(s) 31, 1376, 1409
AseI ATTAAT 1 cut(s) 854
Asp700I GAANNNNTTC 1 cut(s) 951
Asp718I GGTACC 1 cut(s) 1112
AspLEI GCGC 2 cut(s) 107, 607
AspS9I GGNCC 1 cut(s) 1100
AsuC2I CCSGG 1 cut(s) 993
AsuHPI GGTGA 4 cut(s) 220, 922, 952, 1408
AvaI CYCGRG 1 cut(s) 1031
BanI GGYRCC 2 cut(s) 104, 1112
BanII GRGCYC 3 cut(s) 118, 573, 1513
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 43, 1363, 1396
BccI CCATC 7 cut(s) 199, 514, 619, 649, 1288, 1399, 1484
BceAI ACGGC 2 cut(s) 92, 1535
BcgI CGANNNNNNTGC 2 cut(s) 1321, 1355
BciT130I CCWGG 1 cut(s) 602
BciVI GTATCC 1 cut(s) 1315
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 993
BfaI CTAG 3 cut(s) 363, 1157, 1530
BfmI CTRYAG 1 cut(s) 1410
BfoI RGCGCY 2 cut(s) 108, 608
BfuI GTATCC 1 cut(s) 1315
BglI GCCNNNNNGGC 1 cut(s) 113
BisI GCNGC 7 cut(s) 32, 95, 185, 324, 579, 1377, 1410
BlsI GCNGC 7 cut(s) 33, 96, 186, 325, 580, 1378, 1411
Bme1390I CCNGG 2 cut(s) 602, 993
BmeT110I CYCGRG 1 cut(s) 1031
BmgT120I GGNCC 1 cut(s) 1100
BmiI GGNNCC 2 cut(s) 106, 1114
BmrFI CCNGG 2 cut(s) 602, 993
BmrI ACTGGG 1 cut(s) 777
BmsI GCATC 3 cut(s) 171, 280, 517
BmuI ACTGGG 1 cut(s) 777
BpiI GAAGAC 1 cut(s) 837
BplI GAGNNNNNCTC 2 cut(s) 1259, 1291
Bpu10I CCTNAGC 2 cut(s) 83, 507
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 993
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 992
BsaXI ACNNNNNCTCC 6 cut(s) 443, 473, 547, 577, 655, 685
Bsc4I CCNNNNNNNGG 4 cut(s) 248, 249, 893, 1109
Bse1I ACTGG 1 cut(s) 783
Bse3DI GCAATG 1 cut(s) 1529
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 1 cut(s) 992
BseGI GGATG 3 cut(s) 210, 268, 1495
BseLI CCNNNNNNNGG 4 cut(s) 248, 249, 893, 1109
BseMI GCAATG 1 cut(s) 1529
BseMII CTCAG 2 cut(s) 521, 1371
BseNI ACTGG 1 cut(s) 783
BseRI GAGGAG 6 cut(s) 140, 568, 644, 676, 751, 854
BseXI GCAGC 3 cut(s) 43, 1363, 1396
BseYI CCCAGC 3 cut(s) 47, 512, 980
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 1449
BshFI GGCC 3 cut(s) 1089, 1102, 1528
BshNI GGYRCC 2 cut(s) 104, 1112
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 1031
BsiSI CCGG 3 cut(s) 467, 992, 1319
BslFI GGGAC 2 cut(s) 64, 1472
BslI CCNNNNNNNGG 4 cut(s) 248, 249, 893, 1109
BsmFI GGGAC 2 cut(s) 64, 1472
BsmI GAATGC 1 cut(s) 1138
BsnI GGCC 3 cut(s) 1089, 1102, 1528
BsoBI CYCGRG 1 cut(s) 1031
Bsp1286I GDGCHC 4 cut(s) 118, 231, 573, 1513
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 6 cut(s) 72, 597, 889, 1047, 1383, 1422
BspACI CCGC 7 cut(s) 94, 184, 260, 323, 578, 612, 1449
BspANI GGCC 3 cut(s) 1089, 1102, 1528
BspCNI CTCAG 2 cut(s) 520, 1372
BspFNI CGCG 1 cut(s) 1449
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 2 cut(s) 106, 1114
BspMAI CTGCAG 1 cut(s) 1414
BspPI GGATC 1 cut(s) 592
BspT107I GGYRCC 2 cut(s) 104, 1112
BsrDI GCAATG 1 cut(s) 1529
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 1 cut(s) 783
BssECI CCNNGG 1 cut(s) 992
BssMI GATC 6 cut(s) 72, 597, 889, 1047, 1383, 1422
BssNAI GTATAC 1 cut(s) 588
BssNI GRCGYC 1 cut(s) 105
Bst1107I GTATAC 1 cut(s) 588
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 4 cut(s) 160, 595, 910, 1245
BstACI GRCGYC 1 cut(s) 105
BstAUI TGTACA 1 cut(s) 60
BstC8I GCNNGC 5 cut(s) 284, 614, 884, 925, 1273
BstDEI CTNAG 3 cut(s) 83, 507, 1380
BstF5I GGATG 3 cut(s) 210, 268, 1495
BstFNI CGCG 1 cut(s) 1449
BstH2I RGCGCY 2 cut(s) 108, 608
BstHHI GCGC 2 cut(s) 107, 607
BstKTI GATC 6 cut(s) 75, 600, 892, 1050, 1386, 1425
BstMBI GATC 6 cut(s) 72, 597, 889, 1047, 1383, 1422
BstMWI GCNNNNNNNGC 3 cut(s) 113, 613, 1281
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 3 cut(s) 434, 886, 1262
BstSCI CCNGG 2 cut(s) 600, 991
BstSFI CTRYAG 1 cut(s) 1410
BstUI CGCG 1 cut(s) 1449
BstV1I GCAGC 3 cut(s) 43, 1363, 1396
BstV2I GAAGAC 1 cut(s) 837
BstZ17I GTATAC 1 cut(s) 588
BsuI GTATCC 1 cut(s) 1315
BsuRI GGCC 3 cut(s) 1089, 1102, 1528
BtgZI GCGATG 1 cut(s) 650
BtsCI GGATG 3 cut(s) 210, 268, 1495
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 225, 1241
Cac8I GCNNGC 5 cut(s) 284, 614, 884, 925, 1273
CciI TCATGA 1 cut(s) 817
CfoI GCGC 2 cut(s) 107, 607
Cfr13I GGNCC 1 cut(s) 1100
CseI GACGC 1 cut(s) 1455
Csp6I GTAC 4 cut(s) 61, 247, 764, 1113
CviQI GTAC 4 cut(s) 61, 247, 764, 1113
DdeI CTNAG 3 cut(s) 83, 507, 1380
DinI GGCGCC 1 cut(s) 106
DpnI GATC 6 cut(s) 74, 599, 891, 1049, 1385, 1424
DpnII GATC 6 cut(s) 72, 597, 889, 1047, 1383, 1422
DraI TTTAAA 1 cut(s) 1350
DrdI GACNNNNNNGTC 1 cut(s) 1007
DseDI GACNNNNNNGTC 1 cut(s) 1007
EciI GGCGGA 1 cut(s) 275
Eco147I AGGCCT 1 cut(s) 1089
Eco24I GRGCYC 3 cut(s) 118, 573, 1513
Eco88I CYCGRG 1 cut(s) 1031
EcoRII CCWGG 1 cut(s) 600
EcoT22I ATGCAT 1 cut(s) 1222
EcoT38I GRGCYC 3 cut(s) 118, 573, 1513
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
FalI AAGNNNNNCTT 2 cut(s) 818, 850
FaqI GGGAC 2 cut(s) 64, 1472
FauI CCCGC 1 cut(s) 605
FbaI TGATCA 1 cut(s) 72
FblI GTMKAC 1 cut(s) 587
Fnu4HI GCNGC 7 cut(s) 32, 95, 185, 324, 579, 1377, 1410
FokI GGATG 3 cut(s) 217, 275, 1502
FriOI GRGCYC 3 cut(s) 118, 573, 1513
Fsp4HI GCNGC 7 cut(s) 32, 95, 185, 324, 579, 1377, 1410
FspBI CTAG 3 cut(s) 363, 1157, 1530
GlaI GCGC 2 cut(s) 106, 606
GluI GCNGC 7 cut(s) 32, 95, 185, 324, 579, 1377, 1410
GsaI CCCAGC 3 cut(s) 51, 516, 984
HaeII RGCGCY 2 cut(s) 108, 608
HaeIII GGCC 3 cut(s) 1089, 1102, 1528
HapII CCGG 3 cut(s) 467, 992, 1319
HgaI GACGC 1 cut(s) 1455
HhaI GCGC 2 cut(s) 107, 607
Hin1I GRCGYC 1 cut(s) 105
Hin6I GCGC 2 cut(s) 105, 605
HinP1I GCGC 2 cut(s) 105, 605
HindIII AAGCTT 2 cut(s) 413, 1053
HinfI GANTC 3 cut(s) 913, 1018, 1234
HpaII CCGG 3 cut(s) 467, 992, 1319
HphI GGTGA 4 cut(s) 220, 922, 952, 1408
Hpy166II GTNNAC 3 cut(s) 61, 588, 777
Hpy188I TCNGA 1 cut(s) 789
Hpy188III TCNNGA 3 cut(s) 146, 818, 1432
Hpy8I GTNNAC 3 cut(s) 61, 588, 777
HpyAV CCTTC 9 cut(s) 538, 553, 820, 941, 1100, 1114, 1387, 1480, 1491
HpyCH4III ACNGT 4 cut(s) 160, 595, 910, 1245
HpyCH4V TGCA 9 cut(s) 31, 442, 802, 882, 927, 1185, 1220, 1241, 1412
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 613, 1281
HpyF3I CTNAG 3 cut(s) 83, 507, 1380
Hsp92I GRCGYC 1 cut(s) 105
HspAI GCGC 2 cut(s) 105, 605
KasI GGCGCC 1 cut(s) 104
KpnI GGTACC 1 cut(s) 1116
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 6 cut(s) 72, 597, 889, 1047, 1383, 1422
LmnI GCTCC 1 cut(s) 148
Lsp1109I GCAGC 3 cut(s) 43, 1363, 1396
LweI GCATC 3 cut(s) 171, 280, 517
MaeI CTAG 3 cut(s) 363, 1157, 1530
MaeIII GTNAC 3 cut(s) 685, 740, 1189
MalI GATC 6 cut(s) 74, 599, 891, 1049, 1385, 1424
MboI GATC 6 cut(s) 72, 597, 889, 1047, 1383, 1422
MboII GAAGA 8 cut(s) 223, 529, 634, 739, 842, 883, 986, 1495
MhlI GDGCHC 4 cut(s) 118, 231, 573, 1513
MluCI AATT 7 cut(s) 37, 98, 153, 487, 851, 929, 1227
Mly113I GGCGCC 1 cut(s) 105
Mph1103I ATGCAT 1 cut(s) 1222
MroXI GAANNNNTTC 1 cut(s) 951
MseI TTAA 5 cut(s) 854, 1127, 1209, 1349, 1476
MspI CCGG 3 cut(s) 467, 992, 1319
MspR9I CCNGG 2 cut(s) 602, 993
Mva1269I GAATGC 1 cut(s) 1138
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 1 cut(s) 1449
MwoI GCNNNNNNNGC 3 cut(s) 113, 613, 1281
NarI GGCGCC 1 cut(s) 105
NciI CCSGG 1 cut(s) 993
NdeII GATC 6 cut(s) 72, 597, 889, 1047, 1383, 1422
NlaIV GGNNCC 2 cut(s) 106, 1114
NmuCI GTSAC 1 cut(s) 1189
NsiI ATGCAT 1 cut(s) 1222
NspI RCATGY 3 cut(s) 434, 886, 1262
PaeI GCATGC 1 cut(s) 886
PagI TCATGA 1 cut(s) 817
PceI AGGCCT 1 cut(s) 1089
PciI ACATGT 1 cut(s) 430
PcsI WCGNNNNNNNCGW 1 cut(s) 1510
PctI GAATGC 1 cut(s) 1138
PdmI GAANNNNTTC 1 cut(s) 951
PfeI GAWTC 3 cut(s) 913, 1018, 1234
PflMI CCANNNNNTGG 1 cut(s) 1109
PkrI GCNGC 7 cut(s) 33, 96, 186, 325, 580, 1378, 1411
PluTI GGCGCC 1 cut(s) 108
PscI ACATGT 1 cut(s) 430
PshBI ATTAAT 1 cut(s) 854
PsiI TTATAA 1 cut(s) 1362
Psp6I CCWGG 1 cut(s) 600
PspFI CCCAGC 3 cut(s) 47, 512, 980
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 2 cut(s) 106, 1114
PspPI GGNCC 1 cut(s) 1100
PstI CTGCAG 1 cut(s) 1414
RsaI GTAC 4 cut(s) 62, 248, 765, 1114
RsaNI GTAC 4 cut(s) 61, 247, 764, 1113
SaqAI TTAA 5 cut(s) 854, 1127, 1209, 1349, 1476
SatI GCNGC 7 cut(s) 32, 95, 185, 324, 579, 1377, 1410
Sau3AI GATC 6 cut(s) 72, 597, 889, 1047, 1383, 1422
Sau96I GGNCC 1 cut(s) 1100
ScrFI CCNGG 2 cut(s) 602, 993
SduI GDGCHC 4 cut(s) 118, 231, 573, 1513
SfaNI GCATC 3 cut(s) 171, 280, 517
SfcI CTRYAG 1 cut(s) 1410
SfoI GGCGCC 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SphI GCATGC 1 cut(s) 886
Sse9I AATT 7 cut(s) 37, 98, 153, 487, 851, 929, 1227
SseBI AGGCCT 1 cut(s) 1089
SsiI CCGC 7 cut(s) 94, 184, 260, 323, 578, 612, 1449
SspDI GGCGCC 1 cut(s) 104
SspMI CTAG 3 cut(s) 363, 1157, 1530
StuI AGGCCT 1 cut(s) 1089
StyD4I CCNGG 2 cut(s) 600, 991
TaaI ACNGT 4 cut(s) 160, 595, 910, 1245
TaqI TCGA 4 cut(s) 231, 462, 1050, 1513
TasI AATT 7 cut(s) 37, 98, 153, 487, 851, 929, 1227
TatI WGTACW 1 cut(s) 60
TauI GCSGC 4 cut(s) 97, 187, 326, 581
TfiI GAWTC 3 cut(s) 913, 1018, 1234
Tru1I TTAA 5 cut(s) 854, 1127, 1209, 1349, 1476
Tru9I TTAA 5 cut(s) 854, 1127, 1209, 1349, 1476
TscAI CASTG 2 cut(s) 225, 1248
TseFI GTSAC 1 cut(s) 1189
TseI GCWGC 3 cut(s) 31, 1376, 1409
Tsp45I GTSAC 1 cut(s) 1189
TspDTI ATGAA 6 cut(s) 249, 834, 981, 1010, 1145, 1226
TspGWI ACGGA 1 cut(s) 1519
TspRI CASTG 2 cut(s) 225, 1248
Van91I CCANNNNNTGG 1 cut(s) 1109
VspI ATTAAT 1 cut(s) 854
XceI RCATGY 3 cut(s) 434, 886, 1262
XmiI GTMKAC 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 951
XspI CTAG 3 cut(s) 363, 1157, 1530
Zsp2I ATGCAT 1 cut(s) 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.