AT5G66590

SCP / Tpx-1 / Ag5 / PR-1 / Sc7 family of extracellular domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012124
Physical Location & Seq
Forward (+)
26573964 .. 26574959
996 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66590.1

Sequence Viewer

Length: 558 bp
ATGGCTTTAACCCATCACATCATCTTCGTGGCACTTCTTGTCATCTCCGTCAAAGCCATCTCACCGGCGGCGAAACTGAAACCCAAACAAATCGTTTCCACTTCTCCTCCACCTCCTCCAACAATCTCTGCAGCAGCTAAAGCTTTCACCGATGCGCACAACAAGGCCAGAGCCATGGTTGGTGTTCCACCACTAGTTTGGAGCCAGACGTTGGAAGCTGCTGCGAGTCGGTTGGCTCGTTACCAGAGGAACCAAAAGAAGTGTGAGTTCGCGAGTCTAAACCCTGGAAAATACGGCGCGAACCAGCTTTGGGCTAAGGGCTTAGTAGCCGTGACACCGTCTCTTGCTGTGGAGACTTGGGTGAAGGAGAAACCTTTCTACAATTATAAGTCAGACACGTGTGCTGCGAACCACACGTGCGGGGTTTATAAACAAGTCGTCTGGAGAAACTCTAAAGAGCTCGGGTGTGCTCAAGCCACGTGTACGAAAGAGTCAACGGTGTTGACCATTTGTTTTTACAATCCTCCTGGAAATGTAATTGGCCAAAAGCCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP DUF4228

Protein Analysis

185

Amino Acids

20.1

Weight (kDa)

9.7

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 51 - 171 2.8e-17 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66590
fragaria_vesca FvH4_1g14370
malus_domestica MD02G1157200.v1.1 MD02G1157300.v1.1 MD15G1271900.v1.1
prunus_persica Prupe.7G146300_v2.0.a1
pyrus_communis pycom02g12430 pycom02g12440 pycom15g23580
rosa_chinensis RchiOBHm_Chr2g0103071
rosa_laevigata RLG00000017203
rosa_multiflora Rmu_co8460475.1_g000001 Rmu_sc0000388.1_g000013
rosa_rugosa Rorug02G0111900
rosa_samantha Rh2AG160000 Rh2BG166600 Rh2CG166800 Rh2DG165600
rosa_wichuraiana Rw2G012520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 387, 429
Acc16I TGCGCA 1 cut(s) 156
AccB7I CCANNNNNTGG 1 cut(s) 211
AccII CGCG 2 cut(s) 272, 299
AciI CCGC 2 cut(s) 68, 420
AcoI YGGCCR 1 cut(s) 541
AcvI CACGTG 3 cut(s) 399, 417, 480
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 2 cut(s) 211, 310
AflIII ACRYGT 4 cut(s) 396, 398, 414, 479
AhlI ACTAGT 1 cut(s) 193
AjnI CCWGG 2 cut(s) 283, 526
AluBI AGCT 5 cut(s) 137, 143, 218, 307, 460
AluI AGCT 5 cut(s) 137, 143, 218, 307, 460
Alw21I GWGCWC 2 cut(s) 462, 472
Alw26I GTCTC 2 cut(s) 345, 347
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 2 cut(s) 165, 541
ApeKI GCWGC 5 cut(s) 131, 134, 218, 221, 404
Asp700I GAANNNNTTC 1 cut(s) 374
AspLEI GCGC 2 cut(s) 157, 299
AsuHPI GGTGA 3 cut(s) 54, 139, 373
AvaI CYCGRG 1 cut(s) 461
BalI TGGCCA 1 cut(s) 543
BanII GRGCYC 1 cut(s) 462
BbrPI CACGTG 3 cut(s) 399, 417, 480
Bbv12I GWGCWC 2 cut(s) 462, 472
BbvI GCAGC 5 cut(s) 143, 146, 205, 208, 391
BccI CCATC 2 cut(s) 21, 65
BceAI ACGGC 2 cut(s) 310, 314
BciT130I CCWGG 2 cut(s) 285, 528
BcoDI GTCTC 2 cut(s) 345, 347
BcuI ACTAGT 1 cut(s) 193
BfaI CTAG 2 cut(s) 194, 556
BfmI CTRYAG 1 cut(s) 129
BisI GCNGC 6 cut(s) 69, 132, 135, 219, 222, 405
BlsI GCNGC 6 cut(s) 70, 133, 136, 220, 223, 406
Bme1390I CCNGG 2 cut(s) 285, 528
BmeT110I CYCGRG 1 cut(s) 461
BmiI GGNNCC 2 cut(s) 203, 251
BmrFI CCNGG 2 cut(s) 285, 528
BmsI GCATC 1 cut(s) 142
BpmI CTGGAG 1 cut(s) 463
Bpu10I CCTNAGC 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 456
BsaAI YACGTR 3 cut(s) 399, 417, 480
BsaJI CCNNGG 2 cut(s) 174, 283
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 310
Bse118I RCCGGY 1 cut(s) 64
BseBI CCWGG 2 cut(s) 285, 528
BseDI CCNNGG 2 cut(s) 174, 283
BseLI CCNNNNNNNGG 2 cut(s) 211, 310
BseRI GAGGAG 2 cut(s) 96, 105
BseXI GCAGC 5 cut(s) 143, 146, 205, 208, 391
Bsh1236I CGCG 2 cut(s) 272, 299
BshFI GGCC 2 cut(s) 167, 543
BsiHKAI GWGCWC 2 cut(s) 462, 472
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 2 cut(s) 211, 310
BsmAI GTCTC 2 cut(s) 345, 347
BsmBI CGTCTC 1 cut(s) 345
BsnI GGCC 2 cut(s) 167, 543
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 462, 472
Bsp19I CCATGG 1 cut(s) 174
Bsp68I TCGCGA 1 cut(s) 272
BspACI CCGC 2 cut(s) 68, 420
BspANI GGCC 2 cut(s) 167, 543
BspFNI CGCG 2 cut(s) 272, 299
BspLI GGNNCC 2 cut(s) 203, 251
BspMAI CTGCAG 1 cut(s) 133
BsrFI RCCGGY 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 2 cut(s) 174, 283
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 2 cut(s) 285, 528
Bst4CI ACNGT 2 cut(s) 339, 499
BstBAI YACGTR 3 cut(s) 399, 417, 480
BstDEI CTNAG 2 cut(s) 315, 322
BstDSI CCRYGG 1 cut(s) 174
BstFNI CGCG 2 cut(s) 272, 299
BstHHI GCGC 2 cut(s) 157, 299
BstMAI GTCTC 2 cut(s) 345, 347
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNI CCWGG 2 cut(s) 285, 528
BstSCI CCNGG 2 cut(s) 283, 526
BstSFI CTRYAG 1 cut(s) 129
BstUI CGCG 2 cut(s) 272, 299
BstV1I GCAGC 5 cut(s) 143, 146, 205, 208, 391
BstXI CCANNNNNNTGG 2 cut(s) 175, 198
BsuRI GGCC 2 cut(s) 167, 543
BtgI CCRYGG 1 cut(s) 174
BtuMI TCGCGA 1 cut(s) 272
CfoI GCGC 2 cut(s) 157, 299
Cfr10I RCCGGY 1 cut(s) 64
Csp6I GTAC 1 cut(s) 483
CviAII CATG 1 cut(s) 175
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 2 cut(s) 315, 322
EaeI YGGCCR 1 cut(s) 541
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 174
Eco24I GRGCYC 1 cut(s) 462
Eco53kI GAGCTC 1 cut(s) 460
Eco72I CACGTG 3 cut(s) 399, 417, 480
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 460
EcoRII CCWGG 2 cut(s) 283, 526
EcoT14I CCWWGG 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 345
FaeI CATG 1 cut(s) 178
FaiI YATR 3 cut(s) 176, 387, 429
FatI CATG 1 cut(s) 174
FauI CCCGC 1 cut(s) 413
Fnu4HI GCNGC 6 cut(s) 69, 132, 135, 219, 222, 405
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 6 cut(s) 69, 132, 135, 219, 222, 405
FspAI RTGCGCAY 1 cut(s) 156
FspBI CTAG 2 cut(s) 194, 556
FspI TGCGCA 1 cut(s) 156
GlaI GCGC 2 cut(s) 156, 298
GluI GCNGC 6 cut(s) 69, 132, 135, 219, 222, 405
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 2 cut(s) 167, 543
HapII CCGG 1 cut(s) 65
HhaI GCGC 2 cut(s) 157, 299
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 2 cut(s) 155, 297
HinP1I GCGC 2 cut(s) 155, 297
HincII GTYRAC 2 cut(s) 495, 504
HindII GTYRAC 2 cut(s) 495, 504
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 3 cut(s) 226, 274, 491
HpaII CCGG 1 cut(s) 65
HphI GGTGA 3 cut(s) 54, 139, 373
Hpy166II GTNNAC 3 cut(s) 483, 495, 504
Hpy188I TCNGA 1 cut(s) 394
Hpy188III TCNNGA 2 cut(s) 271, 442
Hpy8I GTNNAC 3 cut(s) 483, 495, 504
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 2 cut(s) 339, 499
HpyCH4IV ACGT 4 cut(s) 209, 398, 416, 479
HpyCH4V TGCA 1 cut(s) 131
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 2 cut(s) 315, 322
HpySE526I ACGT 4 cut(s) 209, 398, 416, 479
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 2 cut(s) 155, 297
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 5 cut(s) 143, 146, 205, 208, 391
LweI GCATC 1 cut(s) 142
MaeI CTAG 2 cut(s) 194, 556
MaeII ACGT 4 cut(s) 209, 398, 416, 479
MaeIII GTNAC 2 cut(s) 239, 331
MboII GAAGA 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 462, 472
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 2 cut(s) 382, 537
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 3 cut(s) 235, 283, 500
MmeI TCCRAC 2 cut(s) 143, 192
MnlI CCTC 5 cut(s) 117, 123, 126, 240, 534
Mox20I TGGCCA 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 374
MscI TGGCCA 1 cut(s) 543
MseI TTAA 1 cut(s) 8
MslI CAYNNNNRTG 1 cut(s) 26
Msp20I TGGCCA 1 cut(s) 543
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 285, 528
MvaI CCWGG 2 cut(s) 285, 528
MvnI CGCG 2 cut(s) 272, 299
MwoI GCNNNNNNNGC 1 cut(s) 140
NcoI CCATGG 1 cut(s) 174
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 2 cut(s) 203, 251
NmuCI GTSAC 1 cut(s) 331
NruI TCGCGA 1 cut(s) 272
NsbI TGCGCA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 235, 404
PdmI GAANNNNTTC 1 cut(s) 374
PflFI GACNNNGTC 1 cut(s) 337
PflMI CCANNNNNTGG 1 cut(s) 211
PfoI TCCNGGA 1 cut(s) 526
PkrI GCNGC 6 cut(s) 70, 133, 136, 220, 223, 406
PleI GAGTC 3 cut(s) 234, 282, 499
PmaCI CACGTG 3 cut(s) 399, 417, 480
PmlI CACGTG 3 cut(s) 399, 417, 480
PpsI GAGTC 3 cut(s) 234, 282, 499
Ppu21I YACGTR 3 cut(s) 399, 417, 480
PsiI TTATAA 2 cut(s) 387, 429
Psp124BI GAGCTC 1 cut(s) 462
Psp6I CCWGG 2 cut(s) 283, 526
PspCI CACGTG 3 cut(s) 399, 417, 480
PspGI CCWGG 2 cut(s) 283, 526
PspN4I GGNNCC 2 cut(s) 203, 251
PstI CTGCAG 1 cut(s) 133
PsyI GACNNNGTC 1 cut(s) 337
RruI TCGCGA 1 cut(s) 272
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
RseI CAYNNNNRTG 1 cut(s) 26
SacI GAGCTC 1 cut(s) 462
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 6 cut(s) 69, 132, 135, 219, 222, 405
SchI GAGTC 3 cut(s) 235, 283, 500
ScrFI CCNGG 2 cut(s) 285, 528
SduI GDGCHC 2 cut(s) 462, 472
SfaNI GCATC 1 cut(s) 142
SfcI CTRYAG 1 cut(s) 129
SgrAI CRCCGGYG 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
SpeI ACTAGT 1 cut(s) 193
Sse9I AATT 2 cut(s) 382, 537
SsiI CCGC 2 cut(s) 68, 420
SspMI CTAG 2 cut(s) 194, 556
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 2 cut(s) 283, 526
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 339, 499
TaiI ACGT 4 cut(s) 212, 401, 419, 482
TasI AATT 2 cut(s) 382, 537
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 5 cut(s) 131, 134, 218, 221, 404
Tsp45I GTSAC 1 cut(s) 331
TspGWI ACGGA 1 cut(s) 37
Tth111I GACNNNGTC 1 cut(s) 337
Van91I CCANNNNNTGG 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 2 cut(s) 194, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.