MD02G1157200.v1.1

STS14 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012124
Physical Location & Seq
Reverse (-)
13146446 .. 13146874
429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1157200.v1.1.491

Sequence Viewer

Length: 429 bp
ATGTTGCGGGGGCTCAGGCGCCAAGCTCCAACACTTCAGCCAGAGAATACCTCCAAGTTCACAACCAAGCCAGGGCAGCCGTGGCCCGTGGGTGTTGAGCCGCTCAAGTGGATTGAGTCTCTCGCCAATGCAACAAGTAGGCTAGTGAGGTACCGAAAGATCAACCAAGCCTGCAACTTTGCTATCCTCACCAGTGGCAGCAAGTATGGAGCCAATCAGCTGTGGGCAAGTGGGCAGTCAGTGAGTCCAACCATGGTTGTGGACACTTGGGTCAAAGAGAAGGACTTCTATAACCACAGTGGGAACTCGTGTGCCAAAGCACAGGTGTGGTGTGTACACCCAGGTGGTGTGGAGGAAGTCTTTGAAGCTTGGGTGTGCTCAGGCTACTTGTGTGAAAGAACAGAGCAGCTTAAGTATTTGTTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP DUF4228

Protein Analysis

143

Amino Acids

16.0

Weight (kDa)

9.04

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012124)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66590
fragaria_vesca FvH4_1g14370
malus_domestica MD02G1157200.v1.1 MD02G1157300.v1.1 MD15G1271900.v1.1
prunus_persica Prupe.7G146300_v2.0.a1
pyrus_communis pycom02g12430 pycom02g12440 pycom15g23580
rosa_chinensis RchiOBHm_Chr2g0103071
rosa_laevigata RLG00000017203
rosa_multiflora Rmu_co8460475.1_g000001 Rmu_sc0000388.1_g000013
rosa_rugosa Rorug02G0111900
rosa_samantha Rh2AG160000 Rh2BG166600 Rh2CG166800 Rh2DG165600
rosa_wichuraiana Rw2G012520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
Acc65I GGTACC 1 cut(s) 150
AccB1I GGYRCC 2 cut(s) 18, 150
AccBSI CCGCTC 1 cut(s) 103
AciI CCGC 2 cut(s) 7, 101
AcuI CTGAAG 1 cut(s) 20
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 2 cut(s) 152, 336
AfiI CCNNNNNNNGG 1 cut(s) 72
AflII CTTAAG 1 cut(s) 410
AgsI TTSAA 1 cut(s) 365
AjnI CCWGG 2 cut(s) 70, 340
AleI CACNNNNGTG 2 cut(s) 325, 342
AluBI AGCT 4 cut(s) 26, 220, 368, 409
AluI AGCT 4 cut(s) 26, 220, 368, 409
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 1 cut(s) 123
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 76, 198, 406
Asp700I GAANNNNTTC 1 cut(s) 284
Asp718I GGTACC 1 cut(s) 150
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 181
BanI GGYRCC 2 cut(s) 18, 150
BanII GRGCYC 1 cut(s) 15
BauI CACGAG 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 3 cut(s) 88, 210, 418
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 2 cut(s) 72, 342
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 1 cut(s) 143
BfoI RGCGCY 1 cut(s) 22
BfrI CTTAAG 1 cut(s) 410
BisI GCNGC 4 cut(s) 77, 101, 199, 407
BlsI GCNGC 4 cut(s) 78, 102, 200, 408
Bme1390I CCNGG 2 cut(s) 72, 342
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 3 cut(s) 20, 152, 211
BmrFI CCNGG 2 cut(s) 72, 342
BplI GAGNNNNNCTC 2 cut(s) 35, 67
Bpu10I CCTNAGC 2 cut(s) 14, 379
BpuEI CTTGAG 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 19
BsaJI CCNNGG 5 cut(s) 71, 80, 87, 252, 340
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 192
BseBI CCWGG 2 cut(s) 72, 342
BseDI CCNNGG 5 cut(s) 71, 80, 87, 252, 340
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMII CTCAG 2 cut(s) 28, 393
BseNI ACTGG 1 cut(s) 192
BseXI GCAGC 3 cut(s) 88, 210, 418
BshFI GGCC 1 cut(s) 85
BshNI GGYRCC 2 cut(s) 18, 150
BsiHKAI GWGCWC 1 cut(s) 380
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 1 cut(s) 85
Bsp1286I GDGCHC 2 cut(s) 15, 380
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 1 cut(s) 159
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 2 cut(s) 7, 101
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 2 cut(s) 27, 392
BspLI GGNNCC 3 cut(s) 20, 152, 211
BspT107I GGYRCC 2 cut(s) 18, 150
BspTI CTTAAG 1 cut(s) 410
BsrBI CCGCTC 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 5 cut(s) 71, 80, 87, 252, 340
BssMI GATC 1 cut(s) 159
BssNI GRCGYC 1 cut(s) 19
BssSI CACGAG 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 72, 342
Bst4CI ACNGT 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 19
BstAFI CTTAAG 1 cut(s) 410
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 2 cut(s) 14, 379
BstDSI CCRYGG 3 cut(s) 80, 87, 252
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 1 cut(s) 162
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 1 cut(s) 159
BstMWI GCNNNNNNNGC 2 cut(s) 76, 82
BstNI CCWGG 2 cut(s) 72, 342
BstSCI CCNGG 2 cut(s) 70, 340
BstV1I GCAGC 3 cut(s) 88, 210, 418
BstXI CCANNNNNNTGG 1 cut(s) 259
BsuRI GGCC 1 cut(s) 85
BtgI CCRYGG 3 cut(s) 80, 87, 252
BtsIMutI CAGTG 3 cut(s) 199, 246, 304
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 84
Csp6I GTAC 2 cut(s) 151, 335
CviAII CATG 1 cut(s) 253
CviQI GTAC 2 cut(s) 151, 335
DdeI CTNAG 2 cut(s) 14, 379
DinI GGCGCC 1 cut(s) 20
DpnI GATC 1 cut(s) 161
DpnII GATC 1 cut(s) 159
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 70, 340
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 15
EgeI GGCGCC 1 cut(s) 20
EheI GGCGCC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 1 cut(s) 256
FaiI YATR 4 cut(s) 207, 254, 291, 427
FatI CATG 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 77, 101, 199, 407
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 4 cut(s) 77, 101, 199, 407
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 20
GluI GCNGC 4 cut(s) 77, 101, 199, 407
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 1 cut(s) 85
HhaI GCGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 1 cut(s) 256
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 2 cut(s) 116, 244
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 4 cut(s) 60, 262, 335, 337
Hpy8I GTNNAC 4 cut(s) 60, 262, 335, 337
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4V TGCA 2 cut(s) 131, 174
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 82
HpyF3I CTNAG 2 cut(s) 14, 379
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 1 cut(s) 256
HspAI GCGC 1 cut(s) 19
KasI GGCGCC 1 cut(s) 18
KpnI GGTACC 1 cut(s) 154
Kzo9I GATC 1 cut(s) 159
LmnI GCTCC 2 cut(s) 31, 209
LpnPI CCDG 9 cut(s) 54, 57, 84, 184, 205, 308, 327, 354, 366
Lsp1109I GCAGC 3 cut(s) 88, 210, 418
MaeI CTAG 1 cut(s) 143
MalI GATC 1 cut(s) 161
MbiI CCGCTC 1 cut(s) 103
MboI GATC 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 15, 380
Mly113I GGCGCC 1 cut(s) 19
MlyI GAGTC 2 cut(s) 125, 253
MmeI TCCRAC 2 cut(s) 53, 272
MnlI CCTC 4 cut(s) 61, 141, 197, 346
MroXI GAANNNNTTC 1 cut(s) 284
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 3 cut(s) 257, 325, 342
MspA1I CMGCKG 1 cut(s) 220
MspCI CTTAAG 1 cut(s) 410
MspR9I CCNGG 2 cut(s) 72, 342
MvaI CCWGG 2 cut(s) 72, 342
MwoI GCNNNNNNNGC 2 cut(s) 76, 82
NarI GGCGCC 1 cut(s) 19
NcoI CCATGG 1 cut(s) 252
NdeII GATC 1 cut(s) 159
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 3 cut(s) 20, 152, 211
OliI CACNNNNGTG 2 cut(s) 325, 342
PdmI GAANNNNTTC 1 cut(s) 284
PkrI GCNGC 4 cut(s) 78, 102, 200, 408
PleI GAGTC 2 cut(s) 124, 252
PluTI GGCGCC 1 cut(s) 22
PpsI GAGTC 2 cut(s) 124, 252
Psp6I CCWGG 2 cut(s) 70, 340
PspGI CCWGG 2 cut(s) 70, 340
PspN4I GGNNCC 3 cut(s) 20, 152, 211
PspPI GGNCC 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 220
RsaI GTAC 2 cut(s) 152, 336
RsaNI GTAC 2 cut(s) 151, 335
RseI CAYNNNNRTG 3 cut(s) 257, 325, 342
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 4 cut(s) 77, 101, 199, 407
Sau3AI GATC 1 cut(s) 159
Sau96I GGNCC 1 cut(s) 84
SchI GAGTC 2 cut(s) 125, 253
ScrFI CCNGG 2 cut(s) 72, 342
SduI GDGCHC 2 cut(s) 15, 380
SetI ASST 8 cut(s) 28, 53, 152, 222, 327, 346, 370, 411
SfoI GGCGCC 1 cut(s) 20
SmiMI CAYNNNNRTG 3 cut(s) 257, 325, 342
SmlI CTYRAG 2 cut(s) 104, 410
SmoI CTYRAG 2 cut(s) 104, 410
SsiI CCGC 2 cut(s) 7, 101
SspDI GGCGCC 1 cut(s) 18
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 2 cut(s) 70, 340
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 299
TatI WGTACW 1 cut(s) 334
TauI GCSGC 1 cut(s) 103
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 3 cut(s) 199, 246, 304
TseI GCWGC 3 cut(s) 76, 198, 406
TspRI CASTG 3 cut(s) 199, 246, 304
Vha464I CTTAAG 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.