Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0015168

Physical Location & Seq

Genomic Coordinates Forward (+)

26767599 .. 26768956

Gene Structure 1358 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G67070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 390 bp

ATGGCAGCTTCGTCTCTCAATCTCCTCCTCATTCTCTCTCTTCTCACTTTCATTTCTCTCCAGAGATCTGAATCTCTTTCCGATAATCCATCTCTCACTCTTCTACCCGACGGATTCGACTGGCCGATCTCTCACTCCGATGAATTCGATATCATCGACGGTGAAGAAAGCTTCGAAGTTACGGAGGAAGACGACGGCGTAACAGATCGACGGTCATTGTACTGGCGGAGGACGAAGTATTACATATCGTACGGTGCATTGTCGGCGAATAGAGTGCCATGTCCTCCCAGATCTGGAAGATCGTACTACACTCATAACTGCTTCAGAGCTAGAGGTCCCGTTCATCCGTATAGCCGAGGCTGCTCGTCGATCACTCGATGCCGGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

129

Amino Acids

14.73

Weight (kDa)

6.28

Isoelectric Point (pI)

65.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	67 - 129	2.8e-22	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0015168)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67070
fragaria_vesca	FvH4_1g16140
malus_domestica	MD02G1173500.v1.1 MD15G1285000.v1.1
prunus_persica	Prupe.7G132200_v2.0.a1
pyrus_communis	pycom02g13980 pycom15g24770
rosa_chinensis	RchiOBHm_Chr2g0105821
rosa_laevigata	RLG00000017462
rosa_multiflora	Rmu_co8385561.1_g000001
rosa_roxburghii	Rroxscaffold_2G00137870
rosa_rugosa	Rorug02G0130600
rosa_samantha	Rh2BG191400 Rh2CG186100 Rh2DG188000
rosa_wichuraiana	Rw2G014240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	226
AcoI	YGGCCR	1 cut(s)	122
AcsI	RAATTY	1 cut(s)	143
AcuI	CTGAAG	1 cut(s)	307
AfaI	GTAC	3 cut(s)	221, 251, 305
AfiI	CCNNNNNNNGG	1 cut(s)	293
AluBI	AGCT	3 cut(s)	8, 171, 329
AluI	AGCT	3 cut(s)	8, 171, 329
Alw26I	GTCTC	1 cut(s)	18
AoxI	GGCC	1 cut(s)	122
ApeKI	GCWGC	2 cut(s)	5, 360
ApoI	RAATTY	1 cut(s)	143
AspS9I	GGNCC	1 cut(s)	335
AsuHPI	GGTGA	1 cut(s)	173
AsuII	TTCGAA	1 cut(s)	174
AvaII	GGWCC	1 cut(s)	335
BbsI	GAAGAC	1 cut(s)	195
BbvI	GCAGC	2 cut(s)	17, 347
BccI	CCATC	1 cut(s)	97
BceAI	ACGGC	1 cut(s)	211
BcgI	CGANNNNNNTGC	2 cut(s)	256, 290
BcoDI	GTCTC	1 cut(s)	18
BfaI	CTAG	1 cut(s)	330
BglII	AGATCT	2 cut(s)	65, 290
BisI	GCNGC	2 cut(s)	6, 361
BlsI	GCNGC	2 cut(s)	7, 362
Bme18I	GGWCC	1 cut(s)	335
BmgT120I	GGNCC	1 cut(s)	335
BmiI	GGNNCC	1 cut(s)	337
BmsI	GCATC	1 cut(s)	368
BpiI	GAAGAC	1 cut(s)	195
BpmI	CTGGAG	1 cut(s)	44
Bpu14I	TTCGAA	1 cut(s)	174
BsaBI	GATNNNNATC	1 cut(s)	70
BsaJI	CCNNGG	1 cut(s)	355
Bsc4I	CCNNNNNNNGG	1 cut(s)	293
Bse1I	ACTGG	2 cut(s)	125, 227
Bse8I	GATNNNNATC	1 cut(s)	70
BseDI	CCNNGG	1 cut(s)	355
BseGI	GGATG	1 cut(s)	343
BseJI	GATNNNNATC	1 cut(s)	70
BseLI	CCNNNNNNNGG	1 cut(s)	293
BseNI	ACTGG	2 cut(s)	125, 227
BseRI	GAGGAG	2 cut(s)	14, 17
BseXI	GCAGC	2 cut(s)	17, 347
BshFI	GGCC	1 cut(s)	124
BsiSI	CCGG	1 cut(s)	382
BsiWI	CGTACG	1 cut(s)	249
BslFI	GGGAC	1 cut(s)	321
BslI	CCNNNNNNNGG	1 cut(s)	293
BsmAI	GTCTC	1 cut(s)	18
BsmBI	CGTCTC	1 cut(s)	18
BsmFI	GGGAC	1 cut(s)	321
BsnI	GGCC	1 cut(s)	124
Bsp119I	TTCGAA	1 cut(s)	174
Bsp143I	GATC	6 cut(s)	65, 126, 205, 290, 299, 369
BspACI	CCGC	1 cut(s)	226
BspANI	GGCC	1 cut(s)	124
BspLI	GGNNCC	1 cut(s)	337
BspT104I	TTCGAA	1 cut(s)	174
BsrI	ACTGG	2 cut(s)	125, 227
BssECI	CCNNGG	1 cut(s)	355
BssMI	GATC	6 cut(s)	65, 126, 205, 290, 299, 369
Bst4CI	ACNGT	3 cut(s)	161, 213, 254
Bst6I	CTCTTC	2 cut(s)	45, 105
BstBI	TTCGAA	1 cut(s)	174
BstF5I	GGATG	1 cut(s)	343
BstKTI	GATC	6 cut(s)	68, 129, 208, 293, 302, 372
BstMAI	GTCTC	1 cut(s)	18
BstMBI	GATC	6 cut(s)	65, 126, 205, 290, 299, 369
BstMWI	GCNNNNNNNGC	2 cut(s)	263, 360
BstV1I	GCAGC	2 cut(s)	17, 347
BstV2I	GAAGAC	1 cut(s)	195
BstX2I	RGATCY	2 cut(s)	65, 290
BstYI	RGATCY	2 cut(s)	65, 290
BsuRI	GGCC	1 cut(s)	124
BtsCI	GGATG	1 cut(s)	343
Cfr13I	GGNCC	1 cut(s)	335
Csp6I	GTAC	3 cut(s)	220, 250, 304
CviAII	CATG	1 cut(s)	279
CviJI	RGCY	6 cut(s)	8, 124, 171, 329, 354, 360
CviKI_1	RGCY	6 cut(s)	8, 124, 171, 329, 354, 360
CviQI	GTAC	3 cut(s)	220, 250, 304
DpnI	GATC	6 cut(s)	67, 128, 207, 292, 301, 371
DpnII	GATC	6 cut(s)	65, 126, 205, 290, 299, 369
EaeI	YGGCCR	1 cut(s)	122
Eam1104I	CTCTTC	2 cut(s)	45, 105
EarI	CTCTTC	2 cut(s)	45, 105
EciI	GGCGGA	1 cut(s)	241
Eco32I	GATATC	1 cut(s)	151
Eco47I	GGWCC	1 cut(s)	335
Eco57I	CTGAAG	1 cut(s)	307
EcoO109I	RGGNCCY	1 cut(s)	335
EcoRI	GAATTC	1 cut(s)	143
EcoRV	GATATC	1 cut(s)	151
Esp3I	CGTCTC	1 cut(s)	18
FaeI	CATG	1 cut(s)	282
FaiI	YATR	4 cut(s)	245, 280, 315, 351
FaqI	GGGAC	1 cut(s)	321
FatI	CATG	1 cut(s)	278
Fnu4HI	GCNGC	2 cut(s)	6, 361
FokI	GGATG	1 cut(s)	330
Fsp4HI	GCNGC	2 cut(s)	6, 361
FspBI	CTAG	1 cut(s)	330
GluI	GCNGC	2 cut(s)	6, 361
GsuI	CTGGAG	1 cut(s)	44
HaeIII	GGCC	1 cut(s)	124
HapII	CCGG	1 cut(s)	382
Hin1II	CATG	1 cut(s)	282
HindIII	AAGCTT	1 cut(s)	169
HinfI	GANTC	2 cut(s)	71, 114
HpaII	CCGG	1 cut(s)	382
HphI	GGTGA	1 cut(s)	173
Hpy188I	TCNGA	4 cut(s)	70, 82, 139, 326
Hpy188III	TCNNGA	2 cut(s)	61, 294
Hpy99I	CGWCG	5 cut(s)	113, 161, 197, 213, 370
HpyCH4III	ACNGT	3 cut(s)	161, 213, 254
HpyCH4V	TGCA	1 cut(s)	257
HpyF10VI	GCNNNNNNNGC	2 cut(s)	263, 360
Hsp92II	CATG	1 cut(s)	282
Kzo9I	GATC	6 cut(s)	65, 126, 205, 290, 299, 369
LpnPI	CCDG	5 cut(s)	74, 106, 208, 279, 301
Lsp1109I	GCAGC	2 cut(s)	17, 347
LweI	GCATC	1 cut(s)	368
MaeI	CTAG	1 cut(s)	330
MaeIII	GTNAC	2 cut(s)	178, 199
MalI	GATC	6 cut(s)	67, 128, 207, 292, 301, 371
MboI	GATC	6 cut(s)	65, 126, 205, 290, 299, 369
MboII	GAAGA	5 cut(s)	32, 92, 176, 200, 309
MflI	RGATCY	2 cut(s)	65, 290
MluCI	AATT	1 cut(s)	143
MnlI	CCTC	7 cut(s)	35, 38, 178, 222, 294, 326, 350
MslI	CAYNNNNRTG	1 cut(s)	138
MspI	CCGG	1 cut(s)	382
MwoI	GCNNNNNNNGC	2 cut(s)	263, 360
NdeII	GATC	6 cut(s)	65, 126, 205, 290, 299, 369
NlaIII	CATG	1 cut(s)	282
NlaIV	GGNNCC	1 cut(s)	337
NmeAIII	GCCGAG	1 cut(s)	380
NspV	TTCGAA	1 cut(s)	174
PcsI	WCGNNNNNNNCGW	1 cut(s)	153
PfeI	GAWTC	2 cut(s)	71, 114
Pfl23II	CGTACG	1 cut(s)	249
PkrI	GCNGC	2 cut(s)	7, 362
PpuMI	RGGWCCY	1 cut(s)	335
Psp5II	RGGWCCY	1 cut(s)	335
PspLI	CGTACG	1 cut(s)	249
PspN4I	GGNNCC	1 cut(s)	337
PspPI	GGNCC	1 cut(s)	335
PspPPI	RGGWCCY	1 cut(s)	335
PsuI	RGATCY	2 cut(s)	65, 290
RsaI	GTAC	3 cut(s)	221, 251, 305
RsaNI	GTAC	3 cut(s)	220, 250, 304
RseI	CAYNNNNRTG	1 cut(s)	138
SatI	GCNGC	2 cut(s)	6, 361
Sau3AI	GATC	6 cut(s)	65, 126, 205, 290, 299, 369
Sau96I	GGNCC	1 cut(s)	335
SetI	ASST	4 cut(s)	10, 173, 331, 337
SfaNI	GCATC	1 cut(s)	368
SfuI	TTCGAA	1 cut(s)	174
SinI	GGWCC	1 cut(s)	335
SmiMI	CAYNNNNRTG	1 cut(s)	138
Sse9I	AATT	1 cut(s)	143
SsiI	CCGC	1 cut(s)	226
SspMI	CTAG	1 cut(s)	330
TaaI	ACNGT	3 cut(s)	161, 213, 254
TaqI	TCGA	7 cut(s)	117, 147, 156, 174, 208, 368, 376
TasI	AATT	1 cut(s)	143
TatI	WGTACW	1 cut(s)	219
TfiI	GAWTC	2 cut(s)	71, 114
TseI	GCWGC	2 cut(s)	5, 360
TspDTI	ATGAA	3 cut(s)	40, 156, 332
TspGWI	ACGGA	3 cut(s)	126, 197, 336
VpaK11BI	GGWCC	1 cut(s)	335
XapI	RAATTY	1 cut(s)	143
XspI	CTAG	1 cut(s)	330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.