Prupe.7G132200_v2.0.a1

RALF-like 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0015168
Physical Location & Seq
Reverse (-)
15104699 .. 15105451
753 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G132200.1

Sequence Viewer

Length: 408 bp
ATGGCTTCTGCAACCCAACCCAACCTCCTCCTCTGTTTCTTCCTGCTTTTTCTCTACTTGGGTCCATTTGTCAGCGCTCAGATTGAAGAGACCAGCTTGAAGTTGGTGGCAGAGGCTCTGGAGTGGCCTAGCTCCACAATGTTACCACTCTACGACGAGCTCGATGAGGAGGCGGAGGAAGACGGCGGCGAGGACATGGAGCTGGACATAGAACATGGGTACGCACGCAGATCTCTGTTCTGGCGGAGGATGAAGTACTACATTTCGTACGGGGCTCTCTCGGCCAACCGCATCCCCTGCCCGCCGAGGTCAGGGAGGTCTTATTACACCCACAACTGCTTCAAAGCCAGAGGCCCTGTTCGTCCGTACACCCGAGGCTGCTCTAGGATCGCCAGATGCAGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

136

Amino Acids

15.6

Weight (kDa)

6.72

Isoelectric Point (pI)

60.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67070
fragaria_vesca FvH4_1g16140
malus_domestica MD02G1173500.v1.1 MD15G1285000.v1.1
prunus_persica Prupe.7G132200_v2.0.a1
pyrus_communis pycom02g13980 pycom15g24770
rosa_chinensis RchiOBHm_Chr2g0105821
rosa_laevigata RLG00000017462
rosa_multiflora Rmu_co8385561.1_g000001
rosa_roxburghii Rroxscaffold_2G00137870
rosa_rugosa Rorug02G0130600
rosa_samantha Rh2BG191400 Rh2CG186100 Rh2DG188000
rosa_wichuraiana Rw2G014240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 173, 186, 244, 289, 302
AclWI GGATC 1 cut(s) 395
AcoI YGGCCR 1 cut(s) 282
AfaI GTAC 4 cut(s) 221, 257, 269, 368
AfeI AGCGCT 1 cut(s) 76
AfiI CCNNNNNNNGG 1 cut(s) 311
AgsI TTSAA 3 cut(s) 86, 100, 343
AluBI AGCT 4 cut(s) 96, 132, 160, 202
AluI AGCT 4 cut(s) 96, 132, 160, 202
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 1 cut(s) 395
Ama87I CYCGRG 1 cut(s) 372
Aor51HI AGCGCT 1 cut(s) 76
AoxI GGCC 3 cut(s) 125, 282, 352
ApeKI GCWGC 1 cut(s) 378
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 2 cut(s) 62, 353
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 162, 277
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 365
BceAI ACGGC 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 2 cut(s) 129, 384
BfoI RGCGCY 1 cut(s) 78
BglII AGATCT 1 cut(s) 230
BisI GCNGC 2 cut(s) 187, 379
BlsI GCNGC 2 cut(s) 188, 380
BmcAI AGTACT 1 cut(s) 257
Bme18I GGWCC 1 cut(s) 62
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 2 cut(s) 62, 353
BmiI GGNNCC 1 cut(s) 63
BmsI GCATC 2 cut(s) 300, 386
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 140
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 305, 373
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 311
BseDI CCNNGG 2 cut(s) 305, 373
BseGI GGATG 2 cut(s) 255, 291
BseLI CCNNNNNNNGG 1 cut(s) 311
BseMII CTCAG 1 cut(s) 92
BseRI GAGGAG 3 cut(s) 17, 20, 182
BseXI GCAGC 1 cut(s) 365
BshFI GGCC 3 cut(s) 127, 284, 354
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 372
BsiWI CGTACG 1 cut(s) 267
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 83
BsnI GGCC 3 cut(s) 127, 284, 354
Bso31I GGTCTC 1 cut(s) 83
BsoBI CYCGRG 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 162, 277
Bsp143I GATC 2 cut(s) 230, 387
BspACI CCGC 5 cut(s) 173, 186, 244, 289, 302
BspANI GGCC 3 cut(s) 127, 284, 354
BspCNI CTCAG 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 63
BspPI GGATC 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 305, 373
BssMI GATC 2 cut(s) 230, 387
Bst4CI ACNGT 1 cut(s) 405
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 226, 302
BstDEI CTNAG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 255, 291
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 2 cut(s) 233, 390
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 2 cut(s) 230, 387
BstMWI GCNNNNNNNGC 2 cut(s) 281, 297
BstV1I GCAGC 1 cut(s) 365
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 3 cut(s) 127, 284, 354
BtsCI GGATG 2 cut(s) 255, 291
Cac8I GCNNGC 2 cut(s) 226, 302
CfoI GCGC 1 cut(s) 77
Cfr13I GGNCC 2 cut(s) 62, 353
Csp6I GTAC 4 cut(s) 220, 256, 268, 367
CviAII CATG 2 cut(s) 196, 215
CviQI GTAC 4 cut(s) 220, 256, 268, 367
DdeI CTNAG 1 cut(s) 78
DpnI GATC 2 cut(s) 232, 389
DpnII GATC 2 cut(s) 230, 387
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
EciI GGCGGA 2 cut(s) 188, 259
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 2 cut(s) 162, 277
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 62
Eco47III AGCGCT 1 cut(s) 76
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 353
EcoT38I GRGCYC 2 cut(s) 162, 277
FaeI CATG 2 cut(s) 199, 218
FaiI YATR 3 cut(s) 197, 209, 216
FatI CATG 2 cut(s) 195, 214
FauI CCCGC 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 187, 379
FokI GGATG 2 cut(s) 262, 278
FriOI GRGCYC 2 cut(s) 162, 277
Fsp4HI GCNGC 2 cut(s) 187, 379
FspBI CTAG 2 cut(s) 129, 384
GlaI GCGC 1 cut(s) 76
GluI GCNGC 2 cut(s) 187, 379
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 3 cut(s) 127, 284, 354
HhaI GCGC 1 cut(s) 77
Hin1II CATG 2 cut(s) 199, 218
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 1 cut(s) 81
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 1 cut(s) 369
Hpy99I CGWCG 1 cut(s) 158
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4V TGCA 2 cut(s) 11, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 297
HpyF3I CTNAG 1 cut(s) 78
Hsp92II CATG 2 cut(s) 199, 218
HspAI GCGC 1 cut(s) 75
Kzo9I GATC 2 cut(s) 230, 387
LmnI GCTCC 2 cut(s) 137, 199
LpnPI CCDG 9 cut(s) 56, 104, 106, 188, 226, 297, 310, 361, 369
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 2 cut(s) 300, 386
MaeI CTAG 2 cut(s) 129, 384
MaeIII GTNAC 1 cut(s) 141
MalI GATC 2 cut(s) 232, 389
MboI GATC 2 cut(s) 230, 387
MboII GAAGA 3 cut(s) 31, 98, 191
MflI RGATCY 1 cut(s) 230
MhlI GDGCHC 2 cut(s) 162, 277
MwoI GCNNNNNNNGC 2 cut(s) 281, 297
NdeII GATC 2 cut(s) 230, 387
NlaIII CATG 2 cut(s) 199, 218
NlaIV GGNNCC 1 cut(s) 63
NmeAIII GCCGAG 2 cut(s) 260, 330
PcsI WCGNNNNNNNCGW 1 cut(s) 159
Pfl23II CGTACG 1 cut(s) 267
PkrI GCNGC 2 cut(s) 188, 380
Psp124BI GAGCTC 1 cut(s) 162
PspLI CGTACG 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 2 cut(s) 62, 353
PsuI RGATCY 1 cut(s) 230
RsaI GTAC 4 cut(s) 221, 257, 269, 368
RsaNI GTAC 4 cut(s) 220, 256, 268, 367
SacI GAGCTC 1 cut(s) 162
SatI GCNGC 2 cut(s) 187, 379
Sau3AI GATC 2 cut(s) 230, 387
Sau96I GGNCC 2 cut(s) 62, 353
ScaI AGTACT 1 cut(s) 257
SduI GDGCHC 2 cut(s) 162, 277
SetI ASST 7 cut(s) 27, 98, 134, 162, 204, 311, 320
SfaNI GCATC 2 cut(s) 300, 386
SinI GGWCC 1 cut(s) 62
SsiI CCGC 5 cut(s) 173, 186, 244, 289, 302
SspMI CTAG 2 cut(s) 129, 384
SstI GAGCTC 1 cut(s) 162
TaaI ACNGT 1 cut(s) 405
TaqI TCGA 1 cut(s) 162
TatI WGTACW 1 cut(s) 255
TauI GCSGC 1 cut(s) 189
TseI GCWGC 1 cut(s) 378
TspDTI ATGAA 1 cut(s) 266
TspGWI ACGGA 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 62
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XspI CTAG 2 cut(s) 129, 384
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.