FvH4_1g16140

RALF-like 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015168
Physical Location & Seq
Forward (+)
9232850 .. 9233915
1066 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16140.t1

Sequence Viewer

Length: 405 bp
ATGACTTCTTCAACTCTACTCCACTATCTCCTCTGCTTCTTTCTTGCCTTCCTCGTTCTGGGCCCTGACTGCCCTTTCGCCAGGGCCCAGCTCGACGAGGCGAGCGTGAAGTTGGTTACCGACAATTTGGACCTTGCTACCGCAATGACAATGTATCAACAATTCAATGAGGAGGATGGCGAGGACGATGAGGACGAGATGGATGTAGAGAATGGTTACGGTCGTAGATCTCTGTTCTGGAGGAGGATGAGGTACTACATTTCTTACGGTGCTCTCTCGGCGAATAGGATCCCTTGCCCGCCTCGGTCCGGGAGGTCTTACTACACCAACAACTGCTTCAAAGCTAGAGGCCCAGTTCATCCTTACAGCAGAGGCTGCTCTAGGATCACTCGCTGCAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

135

Amino Acids

15.52

Weight (kDa)

7.58

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 67 - 134 2.9e-22 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67070
fragaria_vesca FvH4_1g16140
malus_domestica MD02G1173500.v1.1 MD15G1285000.v1.1
prunus_persica Prupe.7G132200_v2.0.a1
pyrus_communis pycom02g13980 pycom15g24770
rosa_chinensis RchiOBHm_Chr2g0105821
rosa_laevigata RLG00000017462
rosa_multiflora Rmu_co8385561.1_g000001
rosa_roxburghii Rroxscaffold_2G00137870
rosa_rugosa Rorug02G0130600
rosa_samantha Rh2BG191400 Rh2CG186100 Rh2DG188000
rosa_wichuraiana Rw2G014240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 141, 299
AclWI GGATC 3 cut(s) 283, 296, 392
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 2 cut(s) 58, 308
AgsI TTSAA 3 cut(s) 12, 166, 340
AjnI CCWGG 1 cut(s) 80
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 2 cut(s) 91, 344
AluI AGCT 2 cut(s) 91, 344
Alw21I GWGCWC 1 cut(s) 274
AlwI GGATC 3 cut(s) 283, 296, 392
AlwNI CAGNNNCTG 1 cut(s) 375
AoxI GGCC 3 cut(s) 61, 84, 349
ApaI GGGCCC 2 cut(s) 65, 88
ApeKI GCWGC 2 cut(s) 375, 393
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspS9I GGNCC 7 cut(s) 61, 62, 84, 85, 130, 306, 350
AsuC2I CCSGG 1 cut(s) 310
AvaII GGWCC 2 cut(s) 130, 306
BaeGI GKGCMC 2 cut(s) 65, 88
BamHI GGATCC 1 cut(s) 288
BanII GRGCYC 2 cut(s) 65, 88
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 2 cut(s) 362, 380
BccI CCATC 2 cut(s) 170, 193
BciT130I CCWGG 1 cut(s) 82
BcnI CCSGG 1 cut(s) 310
BfaI CTAG 2 cut(s) 345, 381
BfmI CTRYAG 1 cut(s) 394
BglII AGATCT 1 cut(s) 227
BisI GCNGC 2 cut(s) 376, 394
BlsI GCNGC 2 cut(s) 377, 395
Bme1390I CCNGG 2 cut(s) 82, 310
Bme18I GGWCC 2 cut(s) 130, 306
BmgT120I GGNCC 7 cut(s) 61, 62, 84, 85, 130, 306, 350
BmiI GGNNCC 3 cut(s) 63, 86, 290
BmrFI CCNGG 2 cut(s) 82, 310
BmrI ACTGGG 1 cut(s) 347
BmuI ACTGGG 1 cut(s) 347
BpmI CTGGAG 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 81, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 308
Bse1I ACTGG 1 cut(s) 353
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 2 cut(s) 81, 302
BseGI GGATG 4 cut(s) 181, 208, 252, 358
BseLI CCNNNNNNNGG 2 cut(s) 58, 308
BseMI GCAATG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 353
BseRI GAGGAG 3 cut(s) 20, 185, 256
BseSI GKGCMC 2 cut(s) 65, 88
BseXI GCAGC 2 cut(s) 362, 380
BseYI CCCAGC 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 3 cut(s) 63, 86, 351
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 274
BsiSI CCGG 1 cut(s) 309
BslI CCNNNNNNNGG 2 cut(s) 58, 308
BsnI GGCC 3 cut(s) 63, 86, 351
Bsp120I GGGCCC 2 cut(s) 61, 84
Bsp1286I GDGCHC 3 cut(s) 65, 88, 274
Bsp143I GATC 3 cut(s) 227, 288, 384
BspACI CCGC 2 cut(s) 141, 299
BspANI GGCC 3 cut(s) 63, 86, 351
BspLI GGNNCC 3 cut(s) 63, 86, 290
BspMAI CTGCAG 1 cut(s) 398
BspPI GGATC 3 cut(s) 283, 296, 392
BsrDI GCAATG 1 cut(s) 150
BsrI ACTGG 1 cut(s) 353
BssECI CCNNGG 2 cut(s) 81, 302
BssMI GATC 3 cut(s) 227, 288, 384
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 2 cut(s) 221, 269
BstAPI GCANNNNNTGC 1 cut(s) 375
BstC8I GCNNGC 2 cut(s) 103, 299
BstEII GGTNACC 1 cut(s) 115
BstF5I GGATG 4 cut(s) 181, 208, 252, 358
BstKTI GATC 3 cut(s) 230, 291, 387
BstMBI GATC 3 cut(s) 227, 288, 384
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 3 cut(s) 69, 278, 375
BstNI CCWGG 1 cut(s) 82
BstPI GGTNACC 1 cut(s) 115
BstSCI CCNGG 2 cut(s) 80, 308
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 2 cut(s) 65, 88
BstV1I GCAGC 2 cut(s) 362, 380
BstX2I RGATCY 2 cut(s) 227, 288
BstYI RGATCY 2 cut(s) 227, 288
BsuRI GGCC 3 cut(s) 63, 86, 351
BtsCI GGATG 4 cut(s) 181, 208, 252, 358
Cac8I GCNNGC 2 cut(s) 103, 299
CaiI CAGNNNCTG 1 cut(s) 375
Cfr13I GGNCC 7 cut(s) 61, 62, 84, 85, 130, 306, 350
CpoI CGGWCCG 1 cut(s) 306
Csp6I GTAC 1 cut(s) 253
CspI CGGWCCG 1 cut(s) 306
CviJI RGCY 6 cut(s) 63, 86, 91, 344, 351, 375
CviKI_1 RGCY 6 cut(s) 63, 86, 91, 344, 351, 375
CviQI GTAC 1 cut(s) 253
DpnI GATC 3 cut(s) 229, 290, 386
DpnII GATC 3 cut(s) 227, 288, 384
Eco24I GRGCYC 2 cut(s) 65, 88
Eco47I GGWCC 2 cut(s) 130, 306
Eco91I GGTNACC 1 cut(s) 115
EcoO109I RGGNCCY 2 cut(s) 62, 84
EcoO65I GGTNACC 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 80
EcoT38I GRGCYC 2 cut(s) 65, 88
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 376, 394
FokI GGATG 4 cut(s) 188, 215, 259, 345
FriOI GRGCYC 2 cut(s) 65, 88
Fsp4HI GCNGC 2 cut(s) 376, 394
FspBI CTAG 2 cut(s) 345, 381
GluI GCNGC 2 cut(s) 376, 394
GsaI CCCAGC 1 cut(s) 91
GsuI CTGGAG 1 cut(s) 259
HaeIII GGCC 3 cut(s) 63, 86, 351
HapII CCGG 1 cut(s) 309
HpaII CCGG 1 cut(s) 309
Hpy188III TCNNGA 1 cut(s) 238
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 2 cut(s) 58, 393
HpyCH4III ACNGT 2 cut(s) 221, 269
HpyCH4V TGCA 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 278, 375
Kzo9I GATC 3 cut(s) 227, 288, 384
LpnPI CCDG 8 cut(s) 44, 67, 78, 94, 101, 223, 322, 366
Lsp1109I GCAGC 2 cut(s) 362, 380
MaeI CTAG 2 cut(s) 345, 381
MaeIII GTNAC 2 cut(s) 115, 215
MalI GATC 3 cut(s) 229, 290, 386
MboI GATC 3 cut(s) 227, 288, 384
MflI RGATCY 2 cut(s) 227, 288
MhlI GDGCHC 3 cut(s) 65, 88, 274
MluCI AATT 2 cut(s) 124, 161
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 2 cut(s) 82, 310
MvaI CCWGG 1 cut(s) 82
MwoI GCNNNNNNNGC 3 cut(s) 69, 278, 375
NciI CCSGG 1 cut(s) 310
NdeII GATC 3 cut(s) 227, 288, 384
NlaIV GGNNCC 3 cut(s) 63, 86, 290
NmeAIII GCCGAG 1 cut(s) 257
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 192
PfoI TCCNGGA 1 cut(s) 308
PkrI GCNGC 2 cut(s) 377, 395
Psp6I CCWGG 1 cut(s) 80
PspEI GGTNACC 1 cut(s) 115
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 3 cut(s) 63, 86, 290
PspOMI GGGCCC 2 cut(s) 61, 84
PspPI GGNCC 7 cut(s) 61, 62, 84, 85, 130, 306, 350
PstI CTGCAG 1 cut(s) 398
PstNI CAGNNNCTG 1 cut(s) 375
PsuI RGATCY 2 cut(s) 227, 288
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
Rsr2I CGGWCCG 1 cut(s) 306
RsrII CGGWCCG 1 cut(s) 306
SatI GCNGC 2 cut(s) 376, 394
Sau3AI GATC 3 cut(s) 227, 288, 384
Sau96I GGNCC 7 cut(s) 61, 62, 84, 85, 130, 306, 350
ScrFI CCNGG 2 cut(s) 82, 310
SduI GDGCHC 3 cut(s) 65, 88, 274
SetI ASST 6 cut(s) 93, 135, 254, 317, 346, 404
SfcI CTRYAG 1 cut(s) 394
SinI GGWCC 2 cut(s) 130, 306
Sse9I AATT 2 cut(s) 124, 161
SsiI CCGC 2 cut(s) 141, 299
SspMI CTAG 2 cut(s) 345, 381
StyD4I CCNGG 2 cut(s) 80, 308
TaaI ACNGT 2 cut(s) 221, 269
TaqI TCGA 1 cut(s) 93
TaqII GACCGA 1 cut(s) 294
TasI AATT 2 cut(s) 124, 161
TseI GCWGC 2 cut(s) 375, 393
TspDTI ATGAA 1 cut(s) 347
VpaK11BI GGWCC 2 cut(s) 130, 306
XspI CTAG 2 cut(s) 345, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.