RchiOBHm_Chr2g0105821

RALF-like 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015168
Physical Location & Seq
Forward (+)
17072387 .. 17073542
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48000

Sequence Viewer

Length: 585 bp
ATGAAGCTCACAGCTGGCCTCACCTTCCTCTTCCACTCGCATGTGACCCCAGGCCCCCTTTGTCTCTCTCTAACCCCAGAATCTGCCTTGGATTTTCCGAGCTTTAGTTCAAAACCCCAAACACCAACTTACCCTAGTCTCATTGCCCCTTTCTTCCTCTCTCTCACTCTCTCAACTACAATGGCTTCTCCAACTCTACTCCACTACCTCCTTTGCTTCTTCCTCGTCTTCCTCGTTCTGGGCCCTGACGGCTCTTCCGCCGTGGCCCATCTCGACGAAGCGAGCTTGAAGCTAGTAACCGACAATTTGGACCTGGCTAGTGCAATGTCGATGTATCAACAACTGAATGAGGAGGATAAAGAGGACGATGAGGATGAGATAGACGTAGAGAATGGATACGGGCGTAGATCTCTGTTCTGGAGGAGAATGAGGTACTACATTTCTTACGGGGCGCTCTCGGCGAATAGGATCCCATGCCCGCCTCGGTCCGGGAGGTCTTACTACACCAACAACTGCTTCAAGGCTAGAGGCCCAGTTCATCCTTACACCAGAGGCTGCTCTAGGATCACTCGCTGCAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

194

Amino Acids

21.82

Weight (kDa)

7.63

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 133 - 194 2.5e-21 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67070
fragaria_vesca FvH4_1g16140
malus_domestica MD02G1173500.v1.1 MD15G1285000.v1.1
prunus_persica Prupe.7G132200_v2.0.a1
pyrus_communis pycom02g13980 pycom15g24770
rosa_chinensis RchiOBHm_Chr2g0105821
rosa_laevigata RLG00000017462
rosa_multiflora Rmu_co8385561.1_g000001
rosa_roxburghii Rroxscaffold_2G00137870
rosa_rugosa Rorug02G0130600
rosa_samantha Rh2BG191400 Rh2CG186100 Rh2DG188000
rosa_wichuraiana Rw2G014240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 258, 479
AclWI GGATC 3 cut(s) 463, 476, 572
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 238, 488
AgsI TTSAA 3 cut(s) 111, 289, 520
AjnI CCWGG 2 cut(s) 49, 312
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AluBI AGCT 5 cut(s) 7, 14, 102, 285, 292
AluI AGCT 5 cut(s) 7, 14, 102, 285, 292
Alw26I GTCTC 2 cut(s) 68, 143
AlwI GGATC 3 cut(s) 463, 476, 572
AlwNI CAGNNNCTG 2 cut(s) 83, 555
AoxI GGCC 5 cut(s) 16, 52, 241, 264, 529
ApaI GGGCCC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 555, 573
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 7 cut(s) 53, 241, 242, 265, 310, 486, 530
AsuC2I CCSGG 1 cut(s) 490
AsuHPI GGTGA 1 cut(s) 13
AvaII GGWCC 2 cut(s) 310, 486
BaeGI GKGCMC 1 cut(s) 245
BaeI ACNNNNGTAYC 2 cut(s) 388, 421
BamHI GGATCC 1 cut(s) 468
BanII GRGCYC 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 542, 560
BccI CCATC 1 cut(s) 276
BceAI ACGGC 2 cut(s) 245, 265
BciT130I CCWGG 2 cut(s) 51, 314
BciVI GTATCC 1 cut(s) 389
BcnI CCSGG 1 cut(s) 490
BcoDI GTCTC 2 cut(s) 68, 143
BfaI CTAG 5 cut(s) 135, 293, 318, 525, 561
BfmI CTRYAG 1 cut(s) 574
BfoI RGCGCY 1 cut(s) 455
BfuI GTATCC 1 cut(s) 389
BglI GCCNNNNNGGC 1 cut(s) 249
BglII AGATCT 1 cut(s) 407
BisI GCNGC 2 cut(s) 556, 574
BlsI GCNGC 2 cut(s) 557, 575
Bme1390I CCNGG 3 cut(s) 51, 314, 490
Bme18I GGWCC 2 cut(s) 310, 486
BmgT120I GGNCC 7 cut(s) 53, 241, 242, 265, 310, 486, 530
BmiI GGNNCC 3 cut(s) 55, 243, 470
BmrFI CCNGG 3 cut(s) 51, 314, 490
BmrI ACTGGG 1 cut(s) 527
BmuI ACTGGG 1 cut(s) 527
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 439
BpuMI CCSGG 1 cut(s) 490
BsaJI CCNNGG 4 cut(s) 49, 87, 261, 482
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 488
Bse1I ACTGG 1 cut(s) 533
Bse3DI GCAATG 2 cut(s) 141, 330
BseBI CCWGG 2 cut(s) 51, 314
BseDI CCNNGG 4 cut(s) 49, 87, 261, 482
BseGI GGATG 2 cut(s) 379, 538
BseLI CCNNNNNNNGG 2 cut(s) 238, 488
BseMI GCAATG 2 cut(s) 141, 330
BseNI ACTGG 1 cut(s) 533
BseRI GAGGAG 2 cut(s) 365, 436
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 2 cut(s) 542, 560
BshFI GGCC 5 cut(s) 18, 54, 243, 266, 531
BsiSI CCGG 1 cut(s) 489
BslI CCNNNNNNNGG 2 cut(s) 238, 488
BsmAI GTCTC 2 cut(s) 68, 143
BsnI GGCC 5 cut(s) 18, 54, 243, 266, 531
Bsp120I GGGCCC 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 3 cut(s) 407, 468, 564
BspACI CCGC 2 cut(s) 258, 479
BspANI GGCC 5 cut(s) 18, 54, 243, 266, 531
BspLI GGNNCC 3 cut(s) 55, 243, 470
BspMAI CTGCAG 1 cut(s) 578
BspPI GGATC 3 cut(s) 463, 476, 572
BspQI GCTCTTC 1 cut(s) 259
BsrDI GCAATG 2 cut(s) 141, 330
BsrI ACTGG 1 cut(s) 533
BssECI CCNNGG 4 cut(s) 49, 87, 261, 482
BssMI GATC 3 cut(s) 407, 468, 564
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 2 cut(s) 51, 314
Bst6I CTCTTC 2 cut(s) 35, 259
BstC8I GCNNGC 3 cut(s) 16, 283, 479
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 379, 538
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 3 cut(s) 410, 471, 567
BstMAI GTCTC 2 cut(s) 68, 143
BstMBI GATC 3 cut(s) 407, 468, 564
BstMWI GCNNNNNNNGC 2 cut(s) 249, 458
BstNI CCWGG 2 cut(s) 51, 314
BstNSI RCATGY 1 cut(s) 44
BstSCI CCNGG 3 cut(s) 49, 312, 488
BstSFI CTRYAG 1 cut(s) 574
BstSLI GKGCMC 1 cut(s) 245
BstV1I GCAGC 2 cut(s) 542, 560
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 2 cut(s) 407, 468
BstYI RGATCY 2 cut(s) 407, 468
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 5 cut(s) 18, 54, 243, 266, 531
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 379, 538
Cac8I GCNNGC 3 cut(s) 16, 283, 479
CaiI CAGNNNCTG 2 cut(s) 83, 555
CfoI GCGC 1 cut(s) 454
Cfr13I GGNCC 7 cut(s) 53, 241, 242, 265, 310, 486, 530
CpoI CGGWCCG 1 cut(s) 486
Csp6I GTAC 1 cut(s) 433
CspI CGGWCCG 1 cut(s) 486
CviAII CATG 2 cut(s) 41, 474
CviQI GTAC 1 cut(s) 433
DpnI GATC 3 cut(s) 409, 470, 566
DpnII GATC 3 cut(s) 407, 468, 564
Eam1104I CTCTTC 2 cut(s) 35, 259
EarI CTCTTC 2 cut(s) 35, 259
EciI GGCGGA 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 245
Eco47I GGWCC 2 cut(s) 310, 486
EcoO109I RGGNCCY 2 cut(s) 53, 242
EcoRII CCWGG 2 cut(s) 49, 312
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 44, 477
FaiI YATR 2 cut(s) 42, 475
FatI CATG 2 cut(s) 40, 473
FauI CCCGC 1 cut(s) 486
Fnu4HI GCNGC 2 cut(s) 556, 574
FokI GGATG 2 cut(s) 386, 525
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 2 cut(s) 556, 574
FspBI CTAG 5 cut(s) 135, 293, 318, 525, 561
GlaI GCGC 1 cut(s) 453
GluI GCNGC 2 cut(s) 556, 574
GsuI CTGGAG 1 cut(s) 439
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 5 cut(s) 18, 54, 243, 266, 531
HapII CCGG 1 cut(s) 489
HhaI GCGC 1 cut(s) 454
Hin1II CATG 2 cut(s) 44, 477
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HinfI GANTC 1 cut(s) 80
HpaII CCGG 1 cut(s) 489
HphI GGTGA 1 cut(s) 13
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 2 cut(s) 272, 418
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 1 cut(s) 34
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 2 cut(s) 323, 576
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 458
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 2 cut(s) 44, 477
HspAI GCGC 1 cut(s) 452
Kzo9I GATC 3 cut(s) 407, 468, 564
LguI GCTCTTC 1 cut(s) 259
Lsp1109I GCAGC 2 cut(s) 542, 560
MaeI CTAG 5 cut(s) 135, 293, 318, 525, 561
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 2 cut(s) 43, 295
MalI GATC 3 cut(s) 409, 470, 566
MboI GATC 3 cut(s) 407, 468, 564
MboII GAAGA 5 cut(s) 22, 145, 211, 220, 246
MflI RGATCY 2 cut(s) 407, 468
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 1 cut(s) 304
MmeI TCCRAC 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 3 cut(s) 51, 314, 490
MvaI CCWGG 2 cut(s) 51, 314
MwoI GCNNNNNNNGC 2 cut(s) 249, 458
NciI CCSGG 1 cut(s) 490
NdeII GATC 3 cut(s) 407, 468, 564
NlaIII CATG 2 cut(s) 44, 477
NlaIV GGNNCC 3 cut(s) 55, 243, 470
NmeAIII GCCGAG 1 cut(s) 437
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 44
PciSI GCTCTTC 1 cut(s) 259
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PfeI GAWTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 488
PkrI GCNGC 2 cut(s) 557, 575
Psp6I CCWGG 2 cut(s) 49, 312
PspGI CCWGG 2 cut(s) 49, 312
PspN4I GGNNCC 3 cut(s) 55, 243, 470
PspOMI GGGCCC 1 cut(s) 241
PspPI GGNCC 7 cut(s) 53, 241, 242, 265, 310, 486, 530
PstI CTGCAG 1 cut(s) 578
PstNI CAGNNNCTG 2 cut(s) 83, 555
PsuI RGATCY 2 cut(s) 407, 468
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 39
Rsr2I CGGWCCG 1 cut(s) 486
RsrII CGGWCCG 1 cut(s) 486
SapI GCTCTTC 1 cut(s) 259
SatI GCNGC 2 cut(s) 556, 574
Sau3AI GATC 3 cut(s) 407, 468, 564
Sau96I GGNCC 7 cut(s) 53, 241, 242, 265, 310, 486, 530
ScrFI CCNGG 3 cut(s) 51, 314, 490
SduI GDGCHC 1 cut(s) 245
SfcI CTRYAG 1 cut(s) 574
SinI GGWCC 2 cut(s) 310, 486
SmiMI CAYNNNNRTG 1 cut(s) 39
Sse9I AATT 1 cut(s) 304
SsiI CCGC 2 cut(s) 258, 479
SspMI CTAG 5 cut(s) 135, 293, 318, 525, 561
StyD4I CCNGG 3 cut(s) 49, 312, 488
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 273, 329
TaqII GACCGA 1 cut(s) 474
TasI AATT 1 cut(s) 304
TfiI GAWTC 1 cut(s) 80
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 2 cut(s) 555, 573
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 2 cut(s) 17, 527
VpaK11BI GGWCC 2 cut(s) 310, 486
XceI RCATGY 1 cut(s) 44
XspI CTAG 5 cut(s) 135, 293, 318, 525, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.