Rh2CG186100

RALF-like 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015168
Physical Location & Seq
Forward (+)
17316059 .. 17317001
943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG186100.1

Sequence Viewer

Length: 405 bp
ATGGCTTCTCCAACTCTACTCCACTACCTCCTTTGCTTCTTCCTCGTCTTCCTCGTTCTGGGCCCTGACGGCTCTTCCGCCGTGGCCCATCTCGACGAAGCGAGCTTGAAGCTAGTAACCGACAATTTGGACCTGGCTAGTGCAATGTCGATGTATCAACAACTGAATGAGGAGGATAAAGAGGACGATGAGGATGAGATAGACGTAGAGAATGGATACGGGCGTAGATCTCTGTTCTGGAGGAGAATGAGGTACTACATTTCTTACGGGGCGCTCTCGGCGAATAGGATCCCATGCCCGCCTCGGTCCGGGAGGTCTTACTACACCAACAACTGCTTCAAGGCTAGAGGCCCAGTTCATCCTTACACCAGAGGCTGCTCTAGGATCACTCGCTGCAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

134

Amino Acids

15.38

Weight (kDa)

7.64

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 73 - 134 1.1e-21 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67070
fragaria_vesca FvH4_1g16140
malus_domestica MD02G1173500.v1.1 MD15G1285000.v1.1
prunus_persica Prupe.7G132200_v2.0.a1
pyrus_communis pycom02g13980 pycom15g24770
rosa_chinensis RchiOBHm_Chr2g0105821
rosa_laevigata RLG00000017462
rosa_multiflora Rmu_co8385561.1_g000001
rosa_roxburghii Rroxscaffold_2G00137870
rosa_rugosa Rorug02G0130600
rosa_samantha Rh2BG191400 Rh2CG186100 Rh2DG188000
rosa_wichuraiana Rw2G014240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 78, 299
AclWI GGATC 3 cut(s) 283, 296, 392
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 2 cut(s) 58, 308
AgsI TTSAA 2 cut(s) 109, 340
AjnI CCWGG 1 cut(s) 132
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 2 cut(s) 105, 112
AluI AGCT 2 cut(s) 105, 112
AlwI GGATC 3 cut(s) 283, 296, 392
AlwNI CAGNNNCTG 1 cut(s) 375
AoxI GGCC 3 cut(s) 61, 84, 349
ApaI GGGCCC 1 cut(s) 65
ApeKI GCWGC 2 cut(s) 375, 393
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 6 cut(s) 61, 62, 85, 130, 306, 350
AsuC2I CCSGG 1 cut(s) 310
AvaII GGWCC 2 cut(s) 130, 306
BaeGI GKGCMC 1 cut(s) 65
BaeI ACNNNNGTAYC 2 cut(s) 208, 241
BamHI GGATCC 1 cut(s) 288
BanII GRGCYC 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 40
BbvI GCAGC 2 cut(s) 362, 380
BccI CCATC 1 cut(s) 96
BceAI ACGGC 2 cut(s) 65, 85
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 1 cut(s) 209
BcnI CCSGG 1 cut(s) 310
BfaI CTAG 4 cut(s) 113, 138, 345, 381
BfmI CTRYAG 1 cut(s) 394
BfoI RGCGCY 1 cut(s) 275
BfuI GTATCC 1 cut(s) 209
BglI GCCNNNNNGGC 1 cut(s) 69
BglII AGATCT 1 cut(s) 227
BisI GCNGC 2 cut(s) 376, 394
BlsI GCNGC 2 cut(s) 377, 395
Bme1390I CCNGG 2 cut(s) 134, 310
Bme18I GGWCC 2 cut(s) 130, 306
BmgT120I GGNCC 6 cut(s) 61, 62, 85, 130, 306, 350
BmiI GGNNCC 2 cut(s) 63, 290
BmrFI CCNGG 2 cut(s) 134, 310
BmrI ACTGGG 1 cut(s) 347
BmuI ACTGGG 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 81, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 308
Bse1I ACTGG 1 cut(s) 353
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 2 cut(s) 81, 302
BseGI GGATG 2 cut(s) 199, 358
BseLI CCNNNNNNNGG 2 cut(s) 58, 308
BseMI GCAATG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 353
BseRI GAGGAG 2 cut(s) 185, 256
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 2 cut(s) 362, 380
BshFI GGCC 3 cut(s) 63, 86, 351
BsiSI CCGG 1 cut(s) 309
BslI CCNNNNNNNGG 2 cut(s) 58, 308
BsnI GGCC 3 cut(s) 63, 86, 351
Bsp120I GGGCCC 1 cut(s) 61
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 3 cut(s) 227, 288, 384
BspACI CCGC 2 cut(s) 78, 299
BspANI GGCC 3 cut(s) 63, 86, 351
BspLI GGNNCC 2 cut(s) 63, 290
BspMAI CTGCAG 1 cut(s) 398
BspPI GGATC 3 cut(s) 283, 296, 392
BspQI GCTCTTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 150
BsrI ACTGG 1 cut(s) 353
BssECI CCNNGG 2 cut(s) 81, 302
BssMI GATC 3 cut(s) 227, 288, 384
Bst2UI CCWGG 1 cut(s) 134
Bst6I CTCTTC 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 103, 299
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 199, 358
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 3 cut(s) 230, 291, 387
BstMBI GATC 3 cut(s) 227, 288, 384
BstMWI GCNNNNNNNGC 2 cut(s) 69, 278
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 132, 308
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 1 cut(s) 65
BstV1I GCAGC 2 cut(s) 362, 380
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 2 cut(s) 227, 288
BstYI RGATCY 2 cut(s) 227, 288
BsuI GTATCC 1 cut(s) 209
BsuRI GGCC 3 cut(s) 63, 86, 351
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 2 cut(s) 199, 358
Cac8I GCNNGC 2 cut(s) 103, 299
CaiI CAGNNNCTG 1 cut(s) 375
CfoI GCGC 1 cut(s) 274
Cfr13I GGNCC 6 cut(s) 61, 62, 85, 130, 306, 350
CpoI CGGWCCG 1 cut(s) 306
Csp6I GTAC 1 cut(s) 253
CspI CGGWCCG 1 cut(s) 306
CviAII CATG 1 cut(s) 294
CviQI GTAC 1 cut(s) 253
DpnI GATC 3 cut(s) 229, 290, 386
DpnII GATC 3 cut(s) 227, 288, 384
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
EciI GGCGGA 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 2 cut(s) 130, 306
EcoO109I RGGNCCY 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 65
FaeI CATG 1 cut(s) 297
FaiI YATR 1 cut(s) 295
FatI CATG 1 cut(s) 293
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 376, 394
FokI GGATG 2 cut(s) 206, 345
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 2 cut(s) 376, 394
FspBI CTAG 4 cut(s) 113, 138, 345, 381
GlaI GCGC 1 cut(s) 273
GluI GCNGC 2 cut(s) 376, 394
GsuI CTGGAG 1 cut(s) 259
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 3 cut(s) 63, 86, 351
HapII CCGG 1 cut(s) 309
HhaI GCGC 1 cut(s) 274
Hin1II CATG 1 cut(s) 297
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HpaII CCGG 1 cut(s) 309
Hpy188III TCNNGA 2 cut(s) 92, 238
Hpy99I CGWCG 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 2 cut(s) 143, 396
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 278
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 1 cut(s) 297
HspAI GCGC 1 cut(s) 272
Kzo9I GATC 3 cut(s) 227, 288, 384
LguI GCTCTTC 1 cut(s) 79
LpnPI CCDG 8 cut(s) 44, 78, 119, 146, 223, 322, 366, 382
Lsp1109I GCAGC 2 cut(s) 362, 380
MaeI CTAG 4 cut(s) 113, 138, 345, 381
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 115
MalI GATC 3 cut(s) 229, 290, 386
MboI GATC 3 cut(s) 227, 288, 384
MboII GAAGA 3 cut(s) 31, 40, 66
MflI RGATCY 2 cut(s) 227, 288
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 1 cut(s) 124
MmeI TCCRAC 1 cut(s) 35
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 2 cut(s) 134, 310
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 2 cut(s) 69, 278
NciI CCSGG 1 cut(s) 310
NdeII GATC 3 cut(s) 227, 288, 384
NlaIII CATG 1 cut(s) 297
NlaIV GGNNCC 2 cut(s) 63, 290
NmeAIII GCCGAG 1 cut(s) 257
PciSI GCTCTTC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfoI TCCNGGA 1 cut(s) 308
PkrI GCNGC 2 cut(s) 377, 395
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 2 cut(s) 63, 290
PspOMI GGGCCC 1 cut(s) 61
PspPI GGNCC 6 cut(s) 61, 62, 85, 130, 306, 350
PstI CTGCAG 1 cut(s) 398
PstNI CAGNNNCTG 1 cut(s) 375
PsuI RGATCY 2 cut(s) 227, 288
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
Rsr2I CGGWCCG 1 cut(s) 306
RsrII CGGWCCG 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 79
SatI GCNGC 2 cut(s) 376, 394
Sau3AI GATC 3 cut(s) 227, 288, 384
Sau96I GGNCC 6 cut(s) 61, 62, 85, 130, 306, 350
ScrFI CCNGG 2 cut(s) 134, 310
SduI GDGCHC 1 cut(s) 65
SetI ASST 7 cut(s) 30, 107, 114, 135, 207, 254, 317
SfcI CTRYAG 1 cut(s) 394
SinI GGWCC 2 cut(s) 130, 306
Sse9I AATT 1 cut(s) 124
SsiI CCGC 2 cut(s) 78, 299
SspMI CTAG 4 cut(s) 113, 138, 345, 381
StyD4I CCNGG 2 cut(s) 132, 308
TaiI ACGT 1 cut(s) 207
TaqI TCGA 2 cut(s) 93, 149
TaqII GACCGA 1 cut(s) 294
TasI AATT 1 cut(s) 124
TseI GCWGC 2 cut(s) 375, 393
TspDTI ATGAA 1 cut(s) 347
VpaK11BI GGWCC 2 cut(s) 130, 306
XspI CTAG 4 cut(s) 113, 138, 345, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.