MD02G1173500.v1.1

RALF-like 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015168
Physical Location & Seq
Forward (+)
15231979 .. 15232383
405 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1173500.v1.1.491

Sequence Viewer

Length: 405 bp
ATGGCTTCTCCAACTCCTTTCACTCTCCTCCTCTGCTGCTTCCTCATTTTTCTCCATCTGGGTCCTCTCATCCAAGCTCAAATCGACGAAACCAGCTTGAAGCTAGTGACGGAGGGTCTGGACTGGCCGGCCGCCACAATGTCGCTGCTGGACGACGAGCTCGAGGAGGCGGATGACGAGGACGGCGAGGACTTGGAACTGGACGTGGAAAACGCGTACGCGCGCAGATCTCTGTTCTGGAGGAGGATGAAGTACTACATTTCGTACGGCGCGCTGTCGGCAAATCGCATCCCGTGCCCGCCGCGGTCCGGGAGGTCTTATTACACCCACAATTGCTTCAAAGCCAGAGGCCCAGTTCGTCCTTACTTCAGAGGATGCTCTAGAATCGCCCGATGCAGACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

135

Amino Acids

15.42

Weight (kDa)

6.11

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 69 - 134 7.5e-20 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67070
fragaria_vesca FvH4_1g16140
malus_domestica MD02G1173500.v1.1 MD15G1285000.v1.1
prunus_persica Prupe.7G132200_v2.0.a1
pyrus_communis pycom02g13980 pycom15g24770
rosa_chinensis RchiOBHm_Chr2g0105821
rosa_laevigata RLG00000017462
rosa_multiflora Rmu_co8385561.1_g000001
rosa_roxburghii Rroxscaffold_2G00137870
rosa_rugosa Rorug02G0130600
rosa_samantha Rh2BG191400 Rh2CG186100 Rh2DG188000
rosa_wichuraiana Rw2G014240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 215, 221, 223, 272, 304
AciI CCGC 5 cut(s) 132, 170, 299, 302, 304
AcoI YGGCCR 2 cut(s) 125, 129
AcuI CTGAAG 1 cut(s) 352
AfaI GTAC 3 cut(s) 218, 254, 266
AfiI CCNNNNNNNGG 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 2 cut(s) 100, 340
AhdI GACNNNNNGTC 1 cut(s) 114
AjiI CACGTC 1 cut(s) 205
AluBI AGCT 4 cut(s) 77, 96, 103, 160
AluI AGCT 4 cut(s) 77, 96, 103, 160
Alw21I GWGCWC 1 cut(s) 162
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 3 cut(s) 125, 129, 349
ApeKI GCWGC 2 cut(s) 36, 145
AspLEI GCGC 4 cut(s) 223, 225, 272, 274
AspS9I GGNCC 3 cut(s) 62, 306, 350
AsuC2I CCSGG 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 2 cut(s) 62, 306
BaeGI GKGCMC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 23, 132
BccI CCATC 1 cut(s) 63
BceAI ACGGC 2 cut(s) 199, 283
BcnI CCSGG 1 cut(s) 310
BfaI CTAG 2 cut(s) 104, 381
BglII AGATCT 1 cut(s) 227
BisI GCNGC 4 cut(s) 37, 132, 146, 302
BlsI GCNGC 4 cut(s) 38, 133, 147, 303
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 2 cut(s) 62, 306
BmeRI GACNNNNNGTC 1 cut(s) 114
BmeT110I CYCGRG 1 cut(s) 161
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 3 cut(s) 62, 306, 350
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 310
BmrI ACTGGG 1 cut(s) 347
BmsI GCATC 3 cut(s) 297, 365, 383
BmuI ACTGGG 1 cut(s) 347
BpmI CTGGAG 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 310
BsaJI CCNNGG 1 cut(s) 302
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse118I RCCGGY 1 cut(s) 127
Bse1I ACTGG 3 cut(s) 128, 204, 353
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 5 cut(s) 69, 178, 252, 288, 380
BseLI CCNNNNNNNGG 1 cut(s) 308
BseNI ACTGG 3 cut(s) 128, 204, 353
BsePI GCGCGC 2 cut(s) 221, 270
BseRI GAGGAG 4 cut(s) 17, 20, 179, 256
BseSI GKGCMC 1 cut(s) 299
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 2 cut(s) 23, 132
Bsh1236I CGCG 5 cut(s) 215, 221, 223, 272, 304
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 127, 131, 351
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 2 cut(s) 128, 309
BsiWI CGTACG 2 cut(s) 216, 264
BslI CCNNNNNNNGG 1 cut(s) 308
BsnI GGCC 3 cut(s) 127, 131, 351
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 2 cut(s) 162, 299
Bsp143I GATC 1 cut(s) 227
BspACI CCGC 5 cut(s) 132, 170, 299, 302, 304
BspANI GGCC 3 cut(s) 127, 131, 351
BspFNI CGCG 5 cut(s) 215, 221, 223, 272, 304
BspLI GGNNCC 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 127
BsrI ACTGG 3 cut(s) 128, 204, 353
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 1 cut(s) 302
BssHII GCGCGC 2 cut(s) 221, 270
BssMI GATC 1 cut(s) 227
BstAPI GCANNNNNTGC 1 cut(s) 294
BstC8I GCNNGC 4 cut(s) 129, 223, 272, 299
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 5 cut(s) 69, 178, 252, 288, 380
BstFNI CGCG 5 cut(s) 215, 221, 223, 272, 304
BstHHI GCGC 4 cut(s) 223, 225, 272, 274
BstKTI GATC 1 cut(s) 230
BstMBI GATC 1 cut(s) 227
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 278, 294
BstSCI CCNGG 1 cut(s) 308
BstSLI GKGCMC 1 cut(s) 299
BstUI CGCG 5 cut(s) 215, 221, 223, 272, 304
BstV1I GCAGC 2 cut(s) 23, 132
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 3 cut(s) 127, 131, 351
BtgI CCRYGG 1 cut(s) 302
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 5 cut(s) 69, 178, 252, 288, 380
Cac8I GCNNGC 4 cut(s) 129, 223, 272, 299
CfoI GCGC 4 cut(s) 223, 225, 272, 274
Cfr10I RCCGGY 1 cut(s) 127
Cfr13I GGNCC 3 cut(s) 62, 306, 350
Cfr42I CCGCGG 1 cut(s) 305
CpoI CGGWCCG 1 cut(s) 306
Csp6I GTAC 3 cut(s) 217, 253, 265
CspI CGGWCCG 1 cut(s) 306
CviJI RGCY 9 cut(s) 5, 77, 96, 103, 127, 131, 160, 344, 351
CviKI_1 RGCY 9 cut(s) 5, 77, 96, 103, 127, 131, 160, 344, 351
CviQI GTAC 3 cut(s) 217, 253, 265
DpnI GATC 1 cut(s) 229
DpnII GATC 1 cut(s) 227
DriI GACNNNNNGTC 1 cut(s) 114
EaeI YGGCCR 2 cut(s) 125, 129
EagI CGGCCG 1 cut(s) 129
Eam1105I GACNNNNNGTC 1 cut(s) 114
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 160
EclXI CGGCCG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 162
Eco47I GGWCC 2 cut(s) 62, 306
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 162
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 4 cut(s) 37, 132, 146, 302
FokI GGATG 5 cut(s) 56, 185, 259, 275, 387
FriOI GRGCYC 1 cut(s) 162
FseI GGCCGGCC 1 cut(s) 131
Fsp4HI GCNGC 4 cut(s) 37, 132, 146, 302
FspBI CTAG 2 cut(s) 104, 381
GlaI GCGC 4 cut(s) 222, 224, 271, 273
GluI GCNGC 4 cut(s) 37, 132, 146, 302
GsuI CTGGAG 1 cut(s) 259
HaeIII GGCC 3 cut(s) 127, 131, 351
HapII CCGG 2 cut(s) 128, 309
HhaI GCGC 4 cut(s) 223, 225, 272, 274
Hin6I GCGC 4 cut(s) 221, 223, 270, 272
HinP1I GCGC 4 cut(s) 221, 223, 270, 272
HinfI GANTC 1 cut(s) 384
HpaII CCGG 2 cut(s) 128, 309
Hpy188I TCNGA 1 cut(s) 371
Hpy188III TCNNGA 3 cut(s) 119, 238, 381
Hpy99I CGWCG 2 cut(s) 89, 158
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 294
HpySE526I ACGT 1 cut(s) 204
HspAI GCGC 4 cut(s) 221, 223, 270, 272
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
KspI CCGCGG 1 cut(s) 305
Kzo9I GATC 1 cut(s) 227
Lsp1109I GCAGC 2 cut(s) 23, 132
LweI GCATC 3 cut(s) 297, 365, 383
MaeI CTAG 2 cut(s) 104, 381
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 229
MboI GATC 1 cut(s) 227
MfeI CAATTG 1 cut(s) 331
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 2 cut(s) 162, 299
MluCI AATT 1 cut(s) 331
MluI ACGCGT 1 cut(s) 213
MmeI TCCRAC 1 cut(s) 35
MroNI GCCGGC 1 cut(s) 127
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 2 cut(s) 128, 309
MspR9I CCNGG 1 cut(s) 310
MunI CAATTG 1 cut(s) 331
MvnI CGCG 5 cut(s) 215, 221, 223, 272, 304
MwoI GCNNNNNNNGC 2 cut(s) 278, 294
NaeI GCCGGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 310
NdeII GATC 1 cut(s) 227
NgoMIV GCCGGC 1 cut(s) 127
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 106
PaeR7I CTCGAG 1 cut(s) 161
PauI GCGCGC 2 cut(s) 221, 270
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 183
PdiI GCCGGC 1 cut(s) 129
PfeI GAWTC 1 cut(s) 384
Pfl23II CGTACG 2 cut(s) 216, 264
PfoI TCCNGGA 1 cut(s) 308
PkrI GCNGC 4 cut(s) 38, 133, 147, 303
PpuMI RGGWCCY 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 62
PspLI CGTACG 2 cut(s) 216, 264
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 3 cut(s) 62, 306, 350
PspPPI RGGWCCY 1 cut(s) 62
PspXI VCTCGAGB 1 cut(s) 161
PsuI RGATCY 1 cut(s) 227
PteI GCGCGC 2 cut(s) 221, 270
RigI GGCCGGCC 1 cut(s) 131
RsaI GTAC 3 cut(s) 218, 254, 266
RsaNI GTAC 3 cut(s) 217, 253, 265
Rsr2I CGGWCCG 1 cut(s) 306
RsrII CGGWCCG 1 cut(s) 306
SacI GAGCTC 1 cut(s) 162
SacII CCGCGG 1 cut(s) 305
SatI GCNGC 4 cut(s) 37, 132, 146, 302
Sau3AI GATC 1 cut(s) 227
Sau96I GGNCC 3 cut(s) 62, 306, 350
ScaI AGTACT 1 cut(s) 254
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 2 cut(s) 162, 299
SetI ASST 6 cut(s) 79, 98, 105, 162, 207, 317
SfaNI GCATC 3 cut(s) 297, 365, 383
Sfr274I CTCGAG 1 cut(s) 161
Sfr303I CCGCGG 1 cut(s) 305
SgrBI CCGCGG 1 cut(s) 305
SinI GGWCC 2 cut(s) 62, 306
SlaI CTCGAG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 1 cut(s) 331
SsiI CCGC 5 cut(s) 132, 170, 299, 302, 304
SspMI CTAG 2 cut(s) 104, 381
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 1 cut(s) 308
TaiI ACGT 1 cut(s) 207
TaqI TCGA 2 cut(s) 84, 162
TasI AATT 1 cut(s) 331
TatI WGTACW 1 cut(s) 252
TauI GCSGC 2 cut(s) 134, 304
TfiI GAWTC 1 cut(s) 384
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 2 cut(s) 36, 145
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 263
TspGWI ACGGA 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 62, 306
XbaI TCTAGA 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 161
XspI CTAG 2 cut(s) 104, 381
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.