MD15G1285000.v1.1

RALF-like 34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015168
Physical Location & Seq
Forward (+)
25917121 .. 25917525
405 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1285000.v1.1.491

Sequence Viewer

Length: 405 bp
ATGGCTTCTCCAACTGCAACCACTCTTTTCCTCTGCTCCCTCCTCATTTTTCTCCATTTGGGTCCTCAAATCCAAGCTCAAATCGACAAGACCAGCTTGAAGCTAGTTGCGGAGGCTCTGGAGTGGCCGACCGCCACAATGTCGCCACTGTACGATGAGCTCGAGGAGGCAGACGATGAGGACGGCGAGGACTTGGAACTGGACGTGGAAAACGGGTACGCTCGCAGATCTCTGCTCTGGGGGAGGACGAAGTACTACATTTCGTACGGCGCGCTGTCGGCGAATCGCATCCCCTGCCCGCCGCGGTCAGGGAGGTCTTATTACACCCACAGCTGCTTCAAAGCCAGAGGCCCAGTTCGTCCTTACTCCAGAGGATGCTCTAGGATCGCTCTATGCAGACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

135

Amino Acids

15.09

Weight (kDa)

6.72

Isoelectric Point (pI)

51.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 73 - 134 2.8e-19 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67070
fragaria_vesca FvH4_1g16140
malus_domestica MD02G1173500.v1.1 MD15G1285000.v1.1
prunus_persica Prupe.7G132200_v2.0.a1
pyrus_communis pycom02g13980 pycom15g24770
rosa_chinensis RchiOBHm_Chr2g0105821
rosa_laevigata RLG00000017462
rosa_multiflora Rmu_co8385561.1_g000001
rosa_roxburghii Rroxscaffold_2G00137870
rosa_rugosa Rorug02G0130600
rosa_samantha Rh2BG191400 Rh2CG186100 Rh2DG188000
rosa_wichuraiana Rw2G014240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 272, 304
AciI CCGC 5 cut(s) 110, 132, 299, 302, 304
AclWI GGATC 1 cut(s) 392
AcoI YGGCCR 1 cut(s) 125
AfaI GTAC 4 cut(s) 152, 218, 254, 266
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 2 cut(s) 100, 340
AjiI CACGTC 1 cut(s) 205
AluBI AGCT 5 cut(s) 77, 96, 103, 160, 333
AluI AGCT 5 cut(s) 77, 96, 103, 160, 333
Alw21I GWGCWC 1 cut(s) 162
AlwI GGATC 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 2 cut(s) 125, 349
ApeKI GCWGC 1 cut(s) 333
AspLEI GCGC 2 cut(s) 272, 274
AspS9I GGNCC 2 cut(s) 62, 350
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 1 cut(s) 62
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 320
BceAI ACGGC 2 cut(s) 199, 283
BfaI CTAG 2 cut(s) 104, 381
BglII AGATCT 1 cut(s) 227
BisI GCNGC 2 cut(s) 302, 334
BlsI GCNGC 2 cut(s) 303, 335
BmcAI AGTACT 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 62
BmeT110I CYCGRG 1 cut(s) 161
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 62, 350
BmiI GGNNCC 1 cut(s) 63
BmrI ACTGGG 1 cut(s) 347
BmsI GCATC 2 cut(s) 297, 365
BmuI ACTGGG 1 cut(s) 347
BpmI CTGGAG 2 cut(s) 140, 352
BsaJI CCNNGG 1 cut(s) 302
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 204, 353
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 2 cut(s) 288, 380
BseLI CCNNNNNNNGG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 204, 353
BsePI GCGCGC 1 cut(s) 270
BseRI GAGGAG 2 cut(s) 32, 179
BseXI GCAGC 1 cut(s) 320
Bsh1236I CGCG 2 cut(s) 272, 304
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 127, 351
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 161
BsiWI CGTACG 1 cut(s) 264
BslI CCNNNNNNNGG 1 cut(s) 308
BsnI GGCC 2 cut(s) 127, 351
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 2 cut(s) 227, 384
BspACI CCGC 5 cut(s) 110, 132, 299, 302, 304
BspANI GGCC 2 cut(s) 127, 351
BspFNI CGCG 2 cut(s) 272, 304
BspLI GGNNCC 1 cut(s) 63
BspPI GGATC 1 cut(s) 392
BsrI ACTGG 2 cut(s) 204, 353
BssECI CCNNGG 1 cut(s) 302
BssHII GCGCGC 1 cut(s) 270
BssMI GATC 2 cut(s) 227, 384
Bst4CI ACNGT 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 294
BstC8I GCNNGC 3 cut(s) 223, 272, 299
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 2 cut(s) 288, 380
BstFNI CGCG 2 cut(s) 272, 304
BstHHI GCGC 2 cut(s) 272, 274
BstKTI GATC 2 cut(s) 230, 387
BstMBI GATC 2 cut(s) 227, 384
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 278, 294
BstUI CGCG 2 cut(s) 272, 304
BstV1I GCAGC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BsuRI GGCC 2 cut(s) 127, 351
BtgI CCRYGG 1 cut(s) 302
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 2 cut(s) 288, 380
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 3 cut(s) 223, 272, 299
CfoI GCGC 2 cut(s) 272, 274
Cfr13I GGNCC 2 cut(s) 62, 350
Cfr42I CCGCGG 1 cut(s) 305
Csp6I GTAC 4 cut(s) 151, 217, 253, 265
CviQI GTAC 4 cut(s) 151, 217, 253, 265
DpnI GATC 2 cut(s) 229, 386
DpnII GATC 2 cut(s) 227, 384
EaeI YGGCCR 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 62
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 162
FaiI YATR 1 cut(s) 394
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FauI CCCGC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 302, 334
FokI GGATG 2 cut(s) 275, 387
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 2 cut(s) 302, 334
FspBI CTAG 2 cut(s) 104, 381
GlaI GCGC 2 cut(s) 271, 273
GluI GCNGC 2 cut(s) 302, 334
GsuI CTGGAG 2 cut(s) 140, 352
HaeIII GGCC 2 cut(s) 127, 351
HhaI GCGC 2 cut(s) 272, 274
Hin6I GCGC 2 cut(s) 270, 272
HinP1I GCGC 2 cut(s) 270, 272
HinfI GANTC 1 cut(s) 283
Hpy188III TCNNGA 2 cut(s) 119, 369
HpyCH4III ACNGT 1 cut(s) 150
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 2 cut(s) 17, 396
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 294
HpySE526I ACGT 1 cut(s) 204
HspAI GCGC 2 cut(s) 270, 272
KspI CCGCGG 1 cut(s) 305
Kzo9I GATC 2 cut(s) 227, 384
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 9 cut(s) 104, 106, 185, 223, 294, 307, 358, 366, 382
Lsp1109I GCAGC 1 cut(s) 320
LweI GCATC 2 cut(s) 297, 365
MaeI CTAG 2 cut(s) 104, 381
MaeII ACGT 1 cut(s) 204
MalI GATC 2 cut(s) 229, 386
MboI GATC 2 cut(s) 227, 384
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 1 cut(s) 162
MmeI TCCRAC 1 cut(s) 35
MspA1I CMGCKG 2 cut(s) 304, 333
MvnI CGCG 2 cut(s) 272, 304
MwoI GCNNNNNNNGC 2 cut(s) 278, 294
NdeII GATC 2 cut(s) 227, 384
NlaIV GGNNCC 1 cut(s) 63
PaeR7I CTCGAG 1 cut(s) 161
PauI GCGCGC 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PfeI GAWTC 1 cut(s) 283
Pfl23II CGTACG 1 cut(s) 264
PkrI GCNGC 2 cut(s) 303, 335
PpuMI RGGWCCY 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 62
PspLI CGTACG 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 2 cut(s) 62, 350
PspPPI RGGWCCY 1 cut(s) 62
PspXI VCTCGAGB 1 cut(s) 161
PsuI RGATCY 1 cut(s) 227
PteI GCGCGC 1 cut(s) 270
PvuII CAGCTG 1 cut(s) 333
RsaI GTAC 4 cut(s) 152, 218, 254, 266
RsaNI GTAC 4 cut(s) 151, 217, 253, 265
SacI GAGCTC 1 cut(s) 162
SacII CCGCGG 1 cut(s) 305
SatI GCNGC 2 cut(s) 302, 334
Sau3AI GATC 2 cut(s) 227, 384
Sau96I GGNCC 2 cut(s) 62, 350
ScaI AGTACT 1 cut(s) 254
SduI GDGCHC 1 cut(s) 162
SetI ASST 7 cut(s) 79, 98, 105, 162, 207, 317, 335
SfaNI GCATC 2 cut(s) 297, 365
Sfr274I CTCGAG 1 cut(s) 161
Sfr303I CCGCGG 1 cut(s) 305
SgrBI CCGCGG 1 cut(s) 305
SinI GGWCC 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SsiI CCGC 5 cut(s) 110, 132, 299, 302, 304
SspMI CTAG 2 cut(s) 104, 381
SstI GAGCTC 1 cut(s) 162
TaaI ACNGT 1 cut(s) 150
TaiI ACGT 1 cut(s) 207
TaqI TCGA 2 cut(s) 84, 162
TatI WGTACW 1 cut(s) 252
TauI GCSGC 1 cut(s) 304
TfiI GAWTC 1 cut(s) 283
TscAI CASTG 1 cut(s) 153
TseI GCWGC 1 cut(s) 333
TspRI CASTG 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 62
XhoI CTCGAG 1 cut(s) 161
XspI CTAG 2 cut(s) 104, 381
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.