AT5G67130

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011277
Physical Location & Seq
Forward (+)
26791299 .. 26793882
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67130.1

Sequence Viewer

Length: 1281 bp
ATGTCGGCGTGCATCAATGGCTTATGCAGAGCCGTGACTGTCTCTCTTCTTCTTCTTCTTCTTTCTTTCTCTTTTTCCTCAGCTTGCTCCAATGGAAATTGCCAGCTGCTGGATTCATGCTCCTCGGCTACTGATTGTGTCTCTGGTTTATACTGTGGAGACTGTCCTGCCGTTGGAAGAAGTAAACCCGTCTGCACAAGAGGCCAAGCCACAAGTCCAACTTCCATTATCAATGGATTGCCTTTTAACAAGTATACATGGTTGATGACTCATAATGCCTTTAGCAATGCAAATGCACCACTTTTGCCAGGTGTTGAGAGGATTACATTTTACAATCAAGAAGATACTATTACTAATCAGCTACAAAATGGAGTTAGAGGGCTAATGCTGGATATGTATGACTTCAACAATGATATCTGGCTTTGCCATTCGCTTCGAGGGCAATGTTTCAACTTCACCGCTTTTCAACCTGCAATTAACATACTGAGGGAAGTTGAGGCATTTCTGAGTCAAAACCCAACAGAGATTGTTACCATCATAATAGAGGATTATGTGCACAGACCTAAAGGCTTATCAACACTGTTTGCCAACGCTGGTTTGGACAAATACTGGTTCCCTGTTTCGAAAATGCCTAGAAAGGGAGAAGATTGGCCTACTGTGACTGACATGGTGCAAGAGAATCATAGGCTCCTGGTTTTCACTTCTGTTGCTGCCAAAGAAGATGAAGAAGGCGTTGCGTATCAGTGGAGATACATGGTTGAAAATGAATCTGGGGATCCCGGAGTGAAGCGAGGTTCTTGTCCTAACAGAAAAGAGTCTCAACCTTTAAATTCAAAAAGTTCTTCGCTATTCTTGATGAATTACTTCCCGACATATCCGGTTGAGAAAGACGCTTGCAAGGAGCACTCAGCTCCACTTGCTGAGATGGTTGGGACTTGTCTCAAGTCAGGAGGGAATAGAATGCCAAACTTTCTTGCTGTTAACTTCTACATGAGAAGTGATGGAGGCGGCGTTTTCGAAATTCTGGATAGAATGAATGGACCGGTACTTTGCGGTTGTGAAACTCTTTCTGCTTGCCAGCCAGGAGCAGCTTATGGTTCATGCAAAAATGTTACTGTACAGACAAGAACTCCAAGCATGGATTCAACGGCTGGAAGCAACTCAGGTGGAAGCTACTCAGGTTCAGTTCAGTTCTCAAGATCTTTAGCTTCTGTGGCCCAACTAAACAACATTGTTGTCTTCTGCTTCTCATTGCTTCCGTTGCTGATATTTCTACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

46.61

Weight (kDa)

5.81

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 69 - 360 4.6e-153 PI-PLC-like catalytic domain
PI-PLC_X PF26146 109 - 292 2.3e-18 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67130
fragaria_vesca FvH4_1g16250 FvH4_1g16250 FvH4_1g16250
malus_domestica MD02G1174800.v1.1 MD15G1285800.v1.1
prunus_persica Prupe.7G131000_v2.0.a1
pyrus_communis pycom02g14120 pycom15g24890
rosa_chinensis RchiOBHm_Chr2g0105961
rosa_laevigata RLG00000017473
rosa_multiflora Rmu_sc0004367.1_g000025
rosa_roxburghii Rroxscaffold_2G00137520 Rroxscaffold_2G00137670
rosa_rugosa Rorug02G0132600
rosa_samantha Rh2AG182900 Rh2BG193100 Rh2CG187400 Rh2DG189400
rosa_wichuraiana Rw2G014350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 478
AccB7I CCANNNNNTGG 1 cut(s) 109
AccI GTMKAC 1 cut(s) 254
AciI CCGC 3 cut(s) 459, 1008, 1053
AclWI GGATC 2 cut(s) 770, 783
AcsI RAATTY 2 cut(s) 829, 1020
AfaI GTAC 2 cut(s) 1047, 1119
AfiI CCNNNNNNNGG 3 cut(s) 109, 173, 638
AgeI ACCGGT 1 cut(s) 1042
AgsI TTSAA 6 cut(s) 406, 451, 467, 761, 834, 1146
AjnI CCWGG 3 cut(s) 307, 690, 1081
AjuI GAANNNNNNNTTGG 2 cut(s) 1126, 1158
AluBI AGCT 7 cut(s) 83, 106, 361, 911, 1091, 1173, 1208
AluI AGCT 7 cut(s) 83, 106, 361, 911, 1091, 1173, 1208
Alw21I GWGCWC 2 cut(s) 558, 906
Alw26I GTCTC 5 cut(s) 46, 145, 153, 822, 944
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 2 cut(s) 770, 783
AlwNI CAGNNNCTG 1 cut(s) 109
AoxI GGCC 3 cut(s) 202, 650, 1215
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 3 cut(s) 106, 710, 1088
ApoI RAATTY 2 cut(s) 829, 1020
ArsI GACNNNNNNTTYG 2 cut(s) 1032, 1064
AsiGI ACCGGT 1 cut(s) 1042
Asp700I GAANNNNTTC 1 cut(s) 863
AspS9I GGNCC 2 cut(s) 1040, 1216
AsuC2I CCSGG 1 cut(s) 780
AsuHPI GGTGA 1 cut(s) 448
AsuII TTCGAA 2 cut(s) 623, 1017
AvaII GGWCC 1 cut(s) 1040
BaeGI GKGCMC 1 cut(s) 558
BamHI GGATCC 1 cut(s) 775
BbsI GAAGAC 1 cut(s) 1231
Bbv12I GWGCWC 2 cut(s) 558, 906
BbvCI CCTCAGC 1 cut(s) 79
BbvI GCAGC 3 cut(s) 93, 697, 1100
BccI CCATC 3 cut(s) 542, 919, 995
BceAI ACGGC 3 cut(s) 17, 155, 1164
BciT130I CCWGG 3 cut(s) 309, 692, 1083
BcnI CCSGG 1 cut(s) 780
BcoDI GTCTC 5 cut(s) 46, 145, 153, 822, 944
BfaI CTAG 1 cut(s) 633
BfuAI ACCTGC 1 cut(s) 478
BglII AGATCT 1 cut(s) 1199
BisI GCNGC 4 cut(s) 107, 711, 1009, 1089
BlsI GCNGC 4 cut(s) 108, 712, 1010, 1090
Bme1390I CCNGG 4 cut(s) 309, 692, 780, 1083
Bme18I GGWCC 1 cut(s) 1040
BmgT120I GGNCC 2 cut(s) 1040, 1216
BmiI GGNNCC 3 cut(s) 614, 689, 777
BmrFI CCNGG 4 cut(s) 309, 692, 780, 1083
BmsI GCATC 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 1231
Bpu10I CCTNAGC 1 cut(s) 79
Bpu14I TTCGAA 2 cut(s) 623, 1017
BpuEI CTTGAG 2 cut(s) 926, 1180
BpuMI CCSGG 1 cut(s) 780
BsaJI CCNNGG 1 cut(s) 123
BsaWI WCCGGW 2 cut(s) 877, 1042
BsaXI ACNNNNNCTCC 4 cut(s) 996, 1026, 1114, 1144
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 173, 638
Bse118I RCCGGY 1 cut(s) 1042
Bse1I ACTGG 1 cut(s) 614
Bse3DI GCAATG 3 cut(s) 292, 449, 1250
BseBI CCWGG 3 cut(s) 309, 692, 1083
BseDI CCNNGG 1 cut(s) 123
BseLI CCNNNNNNNGG 3 cut(s) 109, 173, 638
BseMI GCAATG 3 cut(s) 292, 449, 1250
BseMII CTCAG 7 cut(s) 93, 476, 497, 912, 921, 1176, 1191
BseNI ACTGG 1 cut(s) 614
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 3 cut(s) 93, 697, 1100
BsgI GTGCAG 1 cut(s) 178
BshFI GGCC 3 cut(s) 204, 652, 1217
BshTI ACCGGT 1 cut(s) 1042
BsiHKAI GWGCWC 2 cut(s) 558, 906
BsiSI CCGG 3 cut(s) 780, 878, 1043
BslFI GGGAC 1 cut(s) 946
BslI CCNNNNNNNGG 3 cut(s) 109, 173, 638
BsmAI GTCTC 5 cut(s) 46, 145, 153, 822, 944
BsmFI GGGAC 1 cut(s) 946
BsmI GAATGC 1 cut(s) 966
BsnI GGCC 3 cut(s) 204, 652, 1217
Bsp119I TTCGAA 2 cut(s) 623, 1017
Bsp1286I GDGCHC 2 cut(s) 558, 906
Bsp1407I TGTACA 1 cut(s) 1117
Bsp143I GATC 2 cut(s) 775, 1199
BspACI CCGC 3 cut(s) 459, 1008, 1053
BspANI GGCC 3 cut(s) 204, 652, 1217
BspCNI CTCAG 7 cut(s) 92, 477, 498, 913, 920, 1175, 1190
BspLI GGNNCC 3 cut(s) 614, 689, 777
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 2 cut(s) 770, 783
BspT104I TTCGAA 2 cut(s) 623, 1017
BsrDI GCAATG 3 cut(s) 292, 449, 1250
BsrFI RCCGGY 1 cut(s) 1042
BsrGI TGTACA 1 cut(s) 1117
BsrI ACTGG 1 cut(s) 614
BssAI RCCGGY 1 cut(s) 1042
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 2 cut(s) 775, 1199
BssNAI GTATAC 1 cut(s) 255
Bst1107I GTATAC 1 cut(s) 255
Bst2UI CCWGG 3 cut(s) 309, 692, 1083
Bst4CI ACNGT 6 cut(s) 40, 155, 164, 582, 658, 1117
Bst6I CTCTTC 1 cut(s) 51
BstAUI TGTACA 1 cut(s) 1117
BstBI TTCGAA 2 cut(s) 623, 1017
BstC8I GCNNGC 6 cut(s) 10, 85, 104, 895, 1075, 1079
BstDEI CTNAG 7 cut(s) 79, 485, 506, 907, 921, 1162, 1177
BstKTI GATC 2 cut(s) 778, 1202
BstMAI GTCTC 5 cut(s) 46, 145, 153, 822, 944
BstMBI GATC 2 cut(s) 775, 1199
BstMWI GCNNNNNNNGC 6 cut(s) 18, 201, 439, 917, 1214, 1261
BstNI CCWGG 3 cut(s) 309, 692, 1083
BstSCI CCNGG 4 cut(s) 307, 690, 778, 1081
BstSLI GKGCMC 1 cut(s) 558
BstV1I GCAGC 3 cut(s) 93, 697, 1100
BstV2I GAAGAC 1 cut(s) 1231
BstX2I RGATCY 2 cut(s) 775, 1199
BstYI RGATCY 2 cut(s) 775, 1199
BstZ17I GTATAC 1 cut(s) 255
BsuRI GGCC 3 cut(s) 204, 652, 1217
BtsIMutI CAGTG 2 cut(s) 578, 749
BveI ACCTGC 1 cut(s) 478
Cac8I GCNNGC 6 cut(s) 10, 85, 104, 895, 1075, 1079
CaiI CAGNNNCTG 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 1042
Cfr13I GGNCC 2 cut(s) 1040, 1216
CseI GACGC 1 cut(s) 899
Csp6I GTAC 2 cut(s) 1046, 1118
CspAI ACCGGT 1 cut(s) 1042
CspCI CAANNNNNGTGG 2 cut(s) 1147, 1182
CviAII CATG 7 cut(s) 117, 258, 667, 754, 991, 1101, 1138
CviQI GTAC 2 cut(s) 1046, 1118
DdeI CTNAG 7 cut(s) 79, 485, 506, 907, 921, 1162, 1177
DpnI GATC 2 cut(s) 777, 1201
DpnII GATC 2 cut(s) 775, 1199
DraI TTTAAA 1 cut(s) 828
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 1040
EcoRII CCWGG 3 cut(s) 307, 690, 1081
EcoRV GATATC 1 cut(s) 415
FaeI CATG 7 cut(s) 120, 261, 670, 757, 994, 1104, 1141
FalI AAGNNNNNCTT 2 cut(s) 205, 237
FaqI GGGAC 1 cut(s) 946
FatI CATG 7 cut(s) 116, 257, 666, 753, 990, 1100, 1137
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 4 cut(s) 107, 711, 1009, 1089
Fsp4HI GCNGC 4 cut(s) 107, 711, 1009, 1089
FspBI CTAG 1 cut(s) 633
GluI GCNGC 4 cut(s) 107, 711, 1009, 1089
HaeIII GGCC 3 cut(s) 204, 652, 1217
HapII CCGG 3 cut(s) 780, 878, 1043
HgaI GACGC 1 cut(s) 899
Hin1II CATG 7 cut(s) 120, 261, 670, 757, 994, 1104, 1141
HincII GTYRAC 1 cut(s) 982
HindII GTYRAC 1 cut(s) 982
HinfI GANTC 7 cut(s) 113, 268, 508, 679, 767, 815, 1142
HpaI GTTAAC 1 cut(s) 982
HpaII CCGG 3 cut(s) 780, 878, 1043
HphI GGTGA 1 cut(s) 448
Hpy166II GTNNAC 4 cut(s) 185, 255, 556, 982
Hpy188I TCNGA 1 cut(s) 507
Hpy188III TCNNGA 6 cut(s) 338, 853, 868, 948, 1025, 1197
Hpy8I GTNNAC 4 cut(s) 185, 255, 556, 982
HpyAV CCTTC 1 cut(s) 722
HpyCH4III ACNGT 6 cut(s) 40, 155, 164, 582, 658, 1117
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 201, 439, 917, 1214, 1261
HpyF3I CTNAG 7 cut(s) 79, 485, 506, 907, 921, 1162, 1177
Hsp92II CATG 7 cut(s) 120, 261, 670, 757, 994, 1104, 1141
KspAI GTTAAC 1 cut(s) 982
Kzo9I GATC 2 cut(s) 775, 1199
LmnI GCTCC 6 cut(s) 92, 125, 693, 901, 916, 1085
Lsp1109I GCAGC 3 cut(s) 93, 697, 1100
LweI GCATC 1 cut(s) 21
MaeI CTAG 1 cut(s) 633
MaeIII GTNAC 4 cut(s) 34, 529, 658, 1111
MalI GATC 2 cut(s) 777, 1201
MboI GATC 2 cut(s) 775, 1199
MflI RGATCY 2 cut(s) 775, 1199
MhlI GDGCHC 2 cut(s) 558, 906
MluCI AATT 5 cut(s) 97, 474, 829, 859, 1020
MlyI GAGTC 3 cut(s) 262, 517, 824
MmeI TCCRAC 2 cut(s) 154, 242
MroXI GAANNNNTTC 1 cut(s) 863
MseI TTAA 4 cut(s) 246, 477, 827, 981
MspA1I CMGCKG 1 cut(s) 106
MspI CCGG 3 cut(s) 780, 878, 1043
MspR9I CCNGG 4 cut(s) 309, 692, 780, 1083
Mva1269I GAATGC 1 cut(s) 966
MvaI CCWGG 3 cut(s) 309, 692, 1083
MwoI GCNNNNNNNGC 6 cut(s) 18, 201, 439, 917, 1214, 1261
NciI CCSGG 1 cut(s) 780
NdeII GATC 2 cut(s) 775, 1199
NlaIII CATG 7 cut(s) 120, 261, 670, 757, 994, 1104, 1141
NlaIV GGNNCC 3 cut(s) 614, 689, 777
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 2 cut(s) 34, 658
NspV TTCGAA 2 cut(s) 623, 1017
PctI GAATGC 1 cut(s) 966
PdmI GAANNNNTTC 1 cut(s) 863
PfeI GAWTC 4 cut(s) 113, 679, 767, 1142
PflMI CCANNNNNTGG 1 cut(s) 109
PfoI TCCNGGA 1 cut(s) 778
PinAI ACCGGT 1 cut(s) 1042
PkrI GCNGC 4 cut(s) 108, 712, 1010, 1090
PleI GAGTC 3 cut(s) 262, 516, 823
PpsI GAGTC 3 cut(s) 262, 516, 823
Psp6I CCWGG 3 cut(s) 307, 690, 1081
PspGI CCWGG 3 cut(s) 307, 690, 1081
PspN4I GGNNCC 3 cut(s) 614, 689, 777
PspPI GGNCC 2 cut(s) 1040, 1216
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 2 cut(s) 775, 1199
PvuII CAGCTG 1 cut(s) 106
RsaI GTAC 2 cut(s) 1047, 1119
RsaNI GTAC 2 cut(s) 1046, 1118
SaqAI TTAA 4 cut(s) 246, 477, 827, 981
SatI GCNGC 4 cut(s) 107, 711, 1009, 1089
Sau3AI GATC 2 cut(s) 775, 1199
Sau96I GGNCC 2 cut(s) 1040, 1216
SchI GAGTC 3 cut(s) 262, 517, 824
ScrFI CCNGG 4 cut(s) 309, 692, 780, 1083
SduI GDGCHC 2 cut(s) 558, 906
SfaNI GCATC 1 cut(s) 21
SfuI TTCGAA 2 cut(s) 623, 1017
SinI GGWCC 1 cut(s) 1040
SmlI CTYRAG 2 cut(s) 941, 1195
SmoI CTYRAG 2 cut(s) 941, 1195
Sse9I AATT 5 cut(s) 97, 474, 829, 859, 1020
SsiI CCGC 3 cut(s) 459, 1008, 1053
SspMI CTAG 1 cut(s) 633
StyD4I CCNGG 4 cut(s) 307, 690, 778, 1081
TaaI ACNGT 6 cut(s) 40, 155, 164, 582, 658, 1117
TaqI TCGA 3 cut(s) 436, 623, 1017
TasI AATT 5 cut(s) 97, 474, 829, 859, 1020
TatI WGTACW 1 cut(s) 1117
TauI GCSGC 1 cut(s) 1011
TfiI GAWTC 4 cut(s) 113, 679, 767, 1142
Tru1I TTAA 4 cut(s) 246, 477, 827, 981
Tru9I TTAA 4 cut(s) 246, 477, 827, 981
TscAI CASTG 2 cut(s) 585, 749
TseFI GTSAC 2 cut(s) 34, 658
TseI GCWGC 3 cut(s) 106, 710, 1088
Tsp45I GTSAC 2 cut(s) 34, 658
TspDTI ATGAA 6 cut(s) 105, 738, 780, 872, 1049, 1089
TspGWI ACGGA 1 cut(s) 1248
TspRI CASTG 2 cut(s) 585, 749
Van91I CCANNNNNTGG 1 cut(s) 109
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 1 cut(s) 1040
XapI RAATTY 2 cut(s) 829, 1020
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XmiI GTMKAC 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 863
XspI CTAG 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.