MD15G1285800.v1.1

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011277
Physical Location & Seq
Reverse (-)
26067400 .. 26070906
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1285800.v1.1.491

Sequence Viewer

Length: 1275 bp
ATGCCACCGTGCTCGGCGGATCACGGCGGCTTATGCATAGCCCCGTCAACCGGCCTCCTCTTCCTGCTACTCTTCTCATTCTTGGCCACCAGCACCACTGCTTGCTCCAACGGCAATTGCCAGGTTTTAGAAGCTTGTGCGGCGGCGACGGACTGCGGACCGGGTCTTTACTGTGGCAACTGCCCTGCTTCCGGTAGGAACCAGCCCGTGTGCACTAGAGGCCAAGCAATTGTTCCCACTTCAATTATTAATGGGTTGCCATTCAACAAGTACACATGGTTGGTGACTCATAATTCTTTCAGCATCGTTGAAGCGCCGCCCGTGGCCGGTGTTCAGAGACTCACATTTTACAACCAAGAGGACACTGTTACTAACCAGTTGAGGAATGGAGTGAGGGGGTTGATGTTGGATATGTACGATTTCGAGAATGATATCTGGCTTTGCCATTCCTTCAAGGGGCAATGTTACAACTTCACTGCATTTCAACCTGCAATTAACACTTTGAAGGAAGTGGAAACATTTCTAAGTCAGAACCCGACAGAGATTGTGACCATTATAATCGAGGACTATGTGAAAACTCCAAAAGGTTTAACAACATTGTTTACCAATGCTGGGTTAGACAAGTATTGGTTTCCTGCTGCGAAAATGCCAATAAAGGGTGAAGAGTGGCCCACAGTTACTGAGATGGTGCAAGAGAATCATCGGCTTCTTGTTTTTACCTCTGATGCTTCAAAGGAAGCACAGGAAGGAGTAGCTTACCAATGGAAGTACATGGTGGAAAATGAAGCTGGAGATCCTGGGGTAAGACCGGGCTCATGCCCCAAGAGAAAAGAATCAAAGCCTCTGAATTCAAAAAGTGCATCTCTTTTCTTTCAAAATTATTTTCCAACATATCCAGTTGAAGCTGAAGCTTGCAAAGAGCATTCAACTCCACTTGAGCAGATGGTCGGTACCTGTTATAAAGCTGCAGGGGTGATGCCAAATTTTTTGGCGGTCAACTTTTATATGAGGAGTGATGGGGGAGGTGTGTTTGATGCTGTTGATAGAATGAATGGCCAAGTGCTTTGCGGGTGTAGTACTGTCAGTGCCTGCCAGGCTGGAGCACCTTCTGGATCTTGCAAGAATATTTCTGTACCAACTAGAAATCCAGTGACCAACAATGCAGGAAGCATTACTGGCTCTGTTCAGTTTTCAAGATCTGCTTCAATACTCTATTCTCCAAATCGCTTGGTTGTCTTCTTGTTTTATATTCCGTTGATGGGATTTTTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

425

Amino Acids

46.2

Weight (kDa)

5.81

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 75 - 365 8e-152 PI-PLC-like catalytic domain
PI-PLC_X PF26146 109 - 287 1.3e-19 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67130
fragaria_vesca FvH4_1g16250 FvH4_1g16250 FvH4_1g16250
malus_domestica MD02G1174800.v1.1 MD15G1285800.v1.1
prunus_persica Prupe.7G131000_v2.0.a1
pyrus_communis pycom02g14120 pycom15g24890
rosa_chinensis RchiOBHm_Chr2g0105961
rosa_laevigata RLG00000017473
rosa_multiflora Rmu_sc0004367.1_g000025
rosa_roxburghii Rroxscaffold_2G00137520 Rroxscaffold_2G00137670
rosa_rugosa Rorug02G0132600
rosa_samantha Rh2AG182900 Rh2BG193100 Rh2CG187400 Rh2DG189400
rosa_wichuraiana Rw2G014350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 557, 960
Acc36I ACCTGC 1 cut(s) 496
Acc65I GGTACC 1 cut(s) 950
AccB1I GGYRCC 1 cut(s) 950
AciI CCGC 8 cut(s) 17, 27, 140, 143, 156, 317, 992, 1068
AclWI GGATC 3 cut(s) 27, 788, 1120
AcoI YGGCCR 3 cut(s) 84, 324, 1054
AcsI RAATTY 2 cut(s) 847, 982
AcuI CTGAAG 1 cut(s) 927
AfaI GTAC 6 cut(s) 272, 416, 770, 952, 1078, 1134
AfiI CCNNNNNNNGG 7 cut(s) 50, 191, 326, 456, 612, 656, 1259
AjnI CCWGG 3 cut(s) 120, 796, 1092
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AluBI AGCT 6 cut(s) 134, 755, 788, 905, 911, 965
AluI AGCT 6 cut(s) 134, 755, 788, 905, 911, 965
Alw21I GWGCWC 3 cut(s) 14, 215, 1105
Alw26I GTCTC 1 cut(s) 331
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 3 cut(s) 27, 788, 1120
AlwNI CAGNNNCTG 2 cut(s) 680, 1089
AoxI GGCC 6 cut(s) 52, 84, 220, 324, 668, 1054
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 2 cut(s) 638, 965
ApoI RAATTY 2 cut(s) 847, 982
AseI ATTAAT 1 cut(s) 249
Asp700I GAANNNNTTC 1 cut(s) 519
Asp718I GGTACC 1 cut(s) 950
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 2 cut(s) 158, 669
AsuC2I CCSGG 2 cut(s) 162, 810
AsuHPI GGTGA 3 cut(s) 295, 671, 985
AvaII GGWCC 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 215
BalI TGGCCA 2 cut(s) 86, 1056
BanI GGYRCC 1 cut(s) 950
BanII GRGCYC 1 cut(s) 815
BbsI GAAGAC 1 cut(s) 1228
Bbv12I GWGCWC 3 cut(s) 14, 215, 1105
BbvI GCAGC 2 cut(s) 625, 952
BccI CCATC 4 cut(s) 679, 937, 1010, 1252
BceAI ACGGC 2 cut(s) 40, 127
BciT130I CCWGG 3 cut(s) 122, 798, 1094
BcnI CCSGG 2 cut(s) 162, 810
BcoDI GTCTC 1 cut(s) 331
BfaI CTAG 2 cut(s) 216, 1140
BfmI CTRYAG 1 cut(s) 966
BfoI RGCGCY 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 496
BglI GCCNNNNNGGC 1 cut(s) 1094
BglII AGATCT 1 cut(s) 1196
BisI GCNGC 6 cut(s) 28, 141, 144, 317, 639, 966
BlsI GCNGC 6 cut(s) 29, 142, 145, 318, 640, 967
BmcAI AGTACT 1 cut(s) 1078
Bme1390I CCNGG 5 cut(s) 122, 162, 798, 810, 1094
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 2 cut(s) 158, 669
BmiI GGNNCC 2 cut(s) 200, 952
BmrFI CCNGG 5 cut(s) 122, 162, 798, 810, 1094
BmsI GCATC 5 cut(s) 312, 715, 869, 966, 1024
BpiI GAAGAC 1 cut(s) 1228
BpmI CTGGAG 2 cut(s) 810, 1119
BpuEI CTTGAG 1 cut(s) 956
BpuMI CCSGG 2 cut(s) 162, 810
BsaJI CCNNGG 2 cut(s) 321, 797
BsaWI WCCGGW 1 cut(s) 191
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 191, 326, 456, 612, 656, 1259
Bse118I RCCGGY 2 cut(s) 50, 326
Bse1I ACTGG 4 cut(s) 376, 896, 1148, 1180
Bse3DI GCAATG 1 cut(s) 467
BseBI CCWGG 3 cut(s) 122, 798, 1094
BseDI CCNNGG 2 cut(s) 321, 797
BseLI CCNNNNNNNGG 7 cut(s) 50, 191, 326, 456, 612, 656, 1259
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 672
BseNI ACTGG 4 cut(s) 376, 896, 1148, 1180
BseRI GAGGAG 2 cut(s) 47, 1024
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 2 cut(s) 625, 952
BseYI CCCAGC 1 cut(s) 611
BshFI GGCC 6 cut(s) 54, 86, 222, 326, 670, 1056
BshNI GGYRCC 1 cut(s) 950
BsiHKAI GWGCWC 3 cut(s) 14, 215, 1105
BsiSI CCGG 5 cut(s) 51, 161, 192, 327, 809
BslI CCNNNNNNNGG 7 cut(s) 50, 191, 326, 456, 612, 656, 1259
BsmAI GTCTC 1 cut(s) 331
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 6 cut(s) 54, 86, 222, 326, 670, 1056
Bsp1286I GDGCHC 4 cut(s) 14, 215, 815, 1105
Bsp143I GATC 4 cut(s) 19, 793, 1112, 1196
BspACI CCGC 8 cut(s) 17, 27, 140, 143, 156, 317, 992, 1068
BspANI GGCC 6 cut(s) 54, 86, 222, 326, 670, 1056
BspCNI CTCAG 1 cut(s) 673
BspLI GGNNCC 2 cut(s) 200, 952
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 496
BspPI GGATC 3 cut(s) 27, 788, 1120
BspT107I GGYRCC 1 cut(s) 950
BsrDI GCAATG 1 cut(s) 467
BsrFI RCCGGY 2 cut(s) 50, 326
BsrI ACTGG 4 cut(s) 376, 896, 1148, 1180
BssAI RCCGGY 2 cut(s) 50, 326
BssECI CCNNGG 2 cut(s) 321, 797
BssMI GATC 4 cut(s) 19, 793, 1112, 1196
Bst2UI CCWGG 3 cut(s) 122, 798, 1094
Bst4CI ACNGT 5 cut(s) 9, 173, 367, 676, 1081
Bst6I CTCTTC 3 cut(s) 65, 77, 657
BstC8I GCNNGC 3 cut(s) 103, 913, 1090
BstDEI CTNAG 2 cut(s) 524, 681
BstDSI CCRYGG 1 cut(s) 321
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 4 cut(s) 22, 796, 1115, 1199
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 4 cut(s) 19, 793, 1112, 1196
BstMWI GCNNNNNNNGC 6 cut(s) 33, 111, 140, 219, 1094, 1176
BstNI CCWGG 3 cut(s) 122, 798, 1094
BstSCI CCNGG 5 cut(s) 120, 160, 796, 808, 1092
BstSFI CTRYAG 1 cut(s) 966
BstSLI GKGCMC 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 625, 952
BstV2I GAAGAC 1 cut(s) 1228
BstX2I RGATCY 3 cut(s) 793, 1112, 1196
BstYI RGATCY 3 cut(s) 793, 1112, 1196
BsuRI GGCC 6 cut(s) 54, 86, 222, 326, 670, 1056
BtgI CCRYGG 1 cut(s) 321
BtsI GCAGTG 2 cut(s) 96, 474
BtsIMutI CAGTG 5 cut(s) 96, 363, 474, 1090, 1155
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 3 cut(s) 103, 913, 1090
CaiI CAGNNNCTG 2 cut(s) 680, 1089
CfoI GCGC 1 cut(s) 316
Cfr10I RCCGGY 2 cut(s) 50, 326
Cfr13I GGNCC 2 cut(s) 158, 669
CpoI CGGWCCG 1 cut(s) 158
Csp6I GTAC 6 cut(s) 271, 415, 769, 951, 1077, 1133
CspI CGGWCCG 1 cut(s) 158
CviAII CATG 3 cut(s) 276, 772, 816
CviQI GTAC 6 cut(s) 271, 415, 769, 951, 1077, 1133
DdeI CTNAG 2 cut(s) 524, 681
DpnI GATC 4 cut(s) 21, 795, 1114, 1198
DpnII GATC 4 cut(s) 19, 793, 1112, 1196
EaeI YGGCCR 3 cut(s) 84, 324, 1054
Eam1104I CTCTTC 3 cut(s) 65, 77, 657
EarI CTCTTC 3 cut(s) 65, 77, 657
EciI GGCGGA 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 815
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 927
EcoRI GAATTC 1 cut(s) 847
EcoRII CCWGG 3 cut(s) 120, 796, 1092
EcoRV GATATC 1 cut(s) 433
EcoT22I ATGCAT 1 cut(s) 38
EcoT38I GRGCYC 1 cut(s) 815
FaeI CATG 3 cut(s) 279, 775, 819
FalI AAGNNNNNCTT 2 cut(s) 1186, 1218
FatI CATG 3 cut(s) 275, 771, 815
FauI CCCGC 1 cut(s) 1061
Fnu4HI GCNGC 6 cut(s) 28, 141, 144, 317, 639, 966
FriOI GRGCYC 1 cut(s) 815
Fsp4HI GCNGC 6 cut(s) 28, 141, 144, 317, 639, 966
FspBI CTAG 2 cut(s) 216, 1140
GlaI GCGC 1 cut(s) 315
GluI GCNGC 6 cut(s) 28, 141, 144, 317, 639, 966
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 2 cut(s) 810, 1119
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 6 cut(s) 54, 86, 222, 326, 670, 1056
HapII CCGG 5 cut(s) 51, 161, 192, 327, 809
HhaI GCGC 1 cut(s) 316
Hin1II CATG 3 cut(s) 279, 775, 819
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 2 cut(s) 48, 997
HindII GTYRAC 2 cut(s) 48, 997
HindIII AAGCTT 2 cut(s) 132, 909
HinfI GANTC 4 cut(s) 286, 339, 697, 833
HpaII CCGG 5 cut(s) 51, 161, 192, 327, 809
HphI GGTGA 3 cut(s) 295, 671, 985
Hpy166II GTNNAC 5 cut(s) 48, 213, 273, 603, 997
Hpy188I TCNGA 4 cut(s) 336, 531, 724, 846
Hpy188III TCNNGA 3 cut(s) 424, 1110, 1194
Hpy8I GTNNAC 5 cut(s) 48, 213, 273, 603, 997
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 4 cut(s) 460, 499, 740, 1116
HpyCH4III ACNGT 5 cut(s) 9, 173, 367, 676, 1081
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 111, 140, 219, 1094, 1176
HpyF3I CTNAG 2 cut(s) 524, 681
Hsp92II CATG 3 cut(s) 279, 775, 819
HspAI GCGC 1 cut(s) 314
KpnI GGTACC 1 cut(s) 954
Kzo9I GATC 4 cut(s) 19, 793, 1112, 1196
LmnI GCTCC 2 cut(s) 110, 1100
Lsp1109I GCAGC 2 cut(s) 625, 952
LweI GCATC 5 cut(s) 312, 715, 869, 966, 1024
MaeI CTAG 2 cut(s) 216, 1140
MaeIII GTNAC 6 cut(s) 283, 367, 464, 547, 676, 1150
MalI GATC 4 cut(s) 21, 795, 1114, 1198
MboI GATC 4 cut(s) 19, 793, 1112, 1196
MboII GAAGA 4 cut(s) 52, 64, 674, 1228
MfeI CAATTG 2 cut(s) 115, 228
MflI RGATCY 3 cut(s) 793, 1112, 1196
MhlI GDGCHC 4 cut(s) 14, 215, 815, 1105
MlsI TGGCCA 2 cut(s) 86, 1056
MluCI AATT 8 cut(s) 115, 228, 243, 292, 492, 847, 877, 982
MluNI TGGCCA 2 cut(s) 86, 1056
MlyI GAGTC 2 cut(s) 280, 333
MmeI TCCRAC 3 cut(s) 132, 387, 911
Mox20I TGGCCA 2 cut(s) 86, 1056
Mph1103I ATGCAT 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 2 cut(s) 86, 1056
MseI TTAA 3 cut(s) 249, 495, 590
Msp20I TGGCCA 2 cut(s) 86, 1056
MspI CCGG 5 cut(s) 51, 161, 192, 327, 809
MspR9I CCNGG 5 cut(s) 122, 162, 798, 810, 1094
MunI CAATTG 2 cut(s) 115, 228
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 3 cut(s) 122, 798, 1094
MwoI GCNNNNNNNGC 6 cut(s) 33, 111, 140, 219, 1094, 1176
NciI CCSGG 2 cut(s) 162, 810
NdeII GATC 4 cut(s) 19, 793, 1112, 1196
NlaIII CATG 3 cut(s) 279, 775, 819
NlaIV GGNNCC 2 cut(s) 200, 952
NmuCI GTSAC 3 cut(s) 283, 547, 1150
NsiI ATGCAT 1 cut(s) 38
PctI GAATGC 1 cut(s) 922
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 2 cut(s) 697, 833
PflFI GACNNNGTC 1 cut(s) 162
PkrI GCNGC 6 cut(s) 29, 142, 145, 318, 640, 967
PleI GAGTC 2 cut(s) 280, 333
PpsI GAGTC 2 cut(s) 280, 333
PshBI ATTAAT 1 cut(s) 249
PsiI TTATAA 2 cut(s) 557, 960
Psp6I CCWGG 3 cut(s) 120, 796, 1092
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 3 cut(s) 120, 796, 1092
PspN4I GGNNCC 2 cut(s) 200, 952
PspPI GGNCC 2 cut(s) 158, 669
PstI CTGCAG 1 cut(s) 970
PstNI CAGNNNCTG 2 cut(s) 680, 1089
PsuI RGATCY 3 cut(s) 793, 1112, 1196
PsyI GACNNNGTC 1 cut(s) 162
RsaI GTAC 6 cut(s) 272, 416, 770, 952, 1078, 1134
RsaNI GTAC 6 cut(s) 271, 415, 769, 951, 1077, 1133
Rsr2I CGGWCCG 1 cut(s) 158
RsrII CGGWCCG 1 cut(s) 158
SaqAI TTAA 3 cut(s) 249, 495, 590
SatI GCNGC 6 cut(s) 28, 141, 144, 317, 639, 966
Sau3AI GATC 4 cut(s) 19, 793, 1112, 1196
Sau96I GGNCC 2 cut(s) 158, 669
ScaI AGTACT 1 cut(s) 1078
SchI GAGTC 2 cut(s) 280, 333
ScrFI CCNGG 5 cut(s) 122, 162, 798, 810, 1094
SduI GDGCHC 4 cut(s) 14, 215, 815, 1105
SfaNI GCATC 5 cut(s) 312, 715, 869, 966, 1024
SfcI CTRYAG 1 cut(s) 966
SinI GGWCC 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 935
SmoI CTYRAG 1 cut(s) 935
Sse9I AATT 8 cut(s) 115, 228, 243, 292, 492, 847, 877, 982
SsiI CCGC 8 cut(s) 17, 27, 140, 143, 156, 317, 992, 1068
SspI AATATT 1 cut(s) 1126
SspMI CTAG 2 cut(s) 216, 1140
StyD4I CCNGG 5 cut(s) 120, 160, 796, 808, 1092
TaaI ACNGT 5 cut(s) 9, 173, 367, 676, 1081
TaqI TCGA 2 cut(s) 423, 561
TasI AATT 8 cut(s) 115, 228, 243, 292, 492, 847, 877, 982
TatI WGTACW 3 cut(s) 270, 768, 1076
TauI GCSGC 4 cut(s) 30, 143, 146, 319
TfiI GAWTC 2 cut(s) 697, 833
Tru1I TTAA 3 cut(s) 249, 495, 590
Tru9I TTAA 3 cut(s) 249, 495, 590
TscAI CASTG 5 cut(s) 103, 370, 481, 1090, 1155
TseFI GTSAC 3 cut(s) 283, 547, 1150
TseI GCWGC 2 cut(s) 638, 965
Tsp45I GTSAC 3 cut(s) 283, 547, 1150
TspDTI ATGAA 2 cut(s) 798, 1064
TspGWI ACGGA 2 cut(s) 164, 1242
TspRI CASTG 5 cut(s) 103, 370, 481, 1090, 1155
Tth111I GACNNNGTC 1 cut(s) 162
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 158
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 2 cut(s) 847, 982
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 216, 1140
ZrmI AGTACT 1 cut(s) 1078
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.