Rroxscaffold_2G00137670

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011277
Physical Location & Seq
Forward (+)
75672632 .. 75674904
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00137670.1

Sequence Viewer

Length: 1140 bp
ATGCTACCCTGTTCACCACACCACCATAGAGCCTTAACCACAATTGGGTACCTCTATCTCCTCCTCTTCTCTGTCTTTGCCCCAACCTCCTCTGCTTGCTCCAATGGGAACTGCCAGCTTCTAGAAGCTTGTACTGCGGCCACGGACTGTGGGCCGGGTCTGTACTGTGGCAACTGCCCTGCTGCAGGCAAGACTCAACCGGTTAATGGCTTACCCTTCAACAAGTACACATGGCTGGTGACTCATAACTCTTTCAGCATCGTCGATGCGCCGCCTTTGCCTGGTGTCCAGAGACTCACATTTTACAATCAGGAAGATACTGTTACTAACCAGTTGAGGAATGGAGTGAGGGGGTTGATGTTGGATATGTATGATTTCGAGAATGATATCTGGCTCTGCCATTCGTTTCGGGGGCAATGCTACAACTTCACTGCGTTTCAACCTGCAATTAACACTTTGACGGAGGTGGAAGCATTTTTAAGCGAGAACCCAACAGAGATTGTGACTATTATAATCGAGGACTATGTGCATACTTCGAAGGGGTTAACAACATTGTTTACTAAGGCTGGGTTGGACAAATATTGGTTTCCTGTATCCAAAATGCCGAGAAAGGGTGAGGACTGGCCCACTGTGACTGAGATGGTGCAAGACAATCACCGGCTTATTGTTTTTACCTCTGCTGCTTCGAAGGAAGCACAGGAAGGAGTAGCTTACCAATGGAAGTACATGGTGGAAAATGAAGCTGGAGATCCTGGGGTCAAACCGGGGTCGTGCCCGAAGAGGAAAGAATCAAAGGCACTGAATTCAAAAAGTGCGTCTCTTTTCTTCCAAAATTATTTCCCAACATATCCAGTTGAAACCGAATCTTGCAAAGAGCATTCAACTCCACTTGAAGCGATGAGGAGTGATGGCGGAGGTGTATTTGATGCTGTTGATAGAATGAATGGACCAACGCTTTGTGGGTGTAGTACTCTCGCTGCCTGCCAGAATGTTTCTGTACCTACTAGAAGTCCAGCATCCAACAATGCAGGAAGCTTTACTGGCTCTGTTCAGTTTGCAAATTCTGCTCCAACACTTTCTTCTCCGAATCGTGTGGTTGCCTTTTTGTTTTATCTTCCACTGATGGCATTTTGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

41.69

Weight (kDa)

5.77

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 65 - 300 3.6e-125 PI-PLC-like catalytic domain
PI-PLC_X PF26146 98 - 248 1.1e-18 PI-PLC X
PI-PLC_cat PF26178 300 - 329 1.5e-06 PI-PLC-like catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67130
fragaria_vesca FvH4_1g16250 FvH4_1g16250 FvH4_1g16250
malus_domestica MD02G1174800.v1.1 MD15G1285800.v1.1
prunus_persica Prupe.7G131000_v2.0.a1
pyrus_communis pycom02g14120 pycom15g24890
rosa_chinensis RchiOBHm_Chr2g0105961
rosa_laevigata RLG00000017473
rosa_multiflora Rmu_sc0004367.1_g000025
rosa_roxburghii Rroxscaffold_2G00137520 Rroxscaffold_2G00137670
rosa_rugosa Rorug02G0132600
rosa_samantha Rh2AG182900 Rh2BG193100 Rh2CG187400 Rh2DG189400
rosa_wichuraiana Rw2G014350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 451
Acc65I GGTACC 1 cut(s) 48
AccB1I GGYRCC 1 cut(s) 48
AciI CCGC 3 cut(s) 137, 272, 912
AclWI GGATC 1 cut(s) 743
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 2 cut(s) 802, 1060
AfaI GTAC 7 cut(s) 50, 133, 164, 227, 725, 970, 999
AfiI CCNNNNNNNGG 5 cut(s) 45, 185, 206, 281, 611
AgeI ACCGGT 1 cut(s) 199
AgsI TTSAA 6 cut(s) 220, 440, 807, 857, 882, 893
AjnI CCWGG 2 cut(s) 280, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 1063, 1095
AluBI AGCT 5 cut(s) 118, 128, 710, 743, 1035
AluI AGCT 5 cut(s) 118, 128, 710, 743, 1035
Alw26I GTCTC 2 cut(s) 286, 822
AlwI GGATC 1 cut(s) 743
AoxI GGCC 3 cut(s) 138, 152, 623
ApeKI GCWGC 3 cut(s) 182, 680, 977
ApoI RAATTY 2 cut(s) 802, 1060
ArsI GACNNNNNNTTYG 4 cut(s) 800, 832, 939, 971
AsiGI ACCGGT 1 cut(s) 199
Asp718I GGTACC 1 cut(s) 48
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 3 cut(s) 152, 624, 947
AsuC2I CCSGG 2 cut(s) 156, 765
AsuHPI GGTGA 4 cut(s) 6, 250, 626, 647
AsuII TTCGAA 2 cut(s) 536, 686
AvaII GGWCC 1 cut(s) 947
BaeGI GKGCMC 1 cut(s) 776
BanI GGYRCC 1 cut(s) 48
BbvI GCAGC 3 cut(s) 169, 667, 964
BccI CCATC 3 cut(s) 634, 902, 1117
BciT130I CCWGG 2 cut(s) 282, 753
BciVI GTATCC 1 cut(s) 604
BcnI CCSGG 2 cut(s) 156, 765
BcoDI GTCTC 2 cut(s) 286, 822
BfaI CTAG 2 cut(s) 122, 1005
BfmI CTRYAG 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 5 cut(s) 138, 183, 272, 681, 978
BlsI GCNGC 5 cut(s) 139, 184, 273, 682, 979
BmcAI AGTACT 1 cut(s) 970
Bme1390I CCNGG 4 cut(s) 156, 282, 753, 765
Bme18I GGWCC 1 cut(s) 947
BmgT120I GGNCC 3 cut(s) 152, 624, 947
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 4 cut(s) 156, 282, 753, 765
BmsI GCATC 4 cut(s) 256, 267, 916, 1025
BpmI CTGGAG 1 cut(s) 765
Bpu14I TTCGAA 2 cut(s) 536, 686
BpuMI CCSGG 2 cut(s) 156, 765
BsaJI CCNNGG 3 cut(s) 141, 752, 764
BsaWI WCCGGW 1 cut(s) 199
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 185, 206, 281, 611
Bse118I RCCGGY 2 cut(s) 199, 657
Bse1I ACTGG 4 cut(s) 331, 626, 851, 1045
Bse3DI GCAATG 1 cut(s) 422
BseBI CCWGG 2 cut(s) 282, 753
BseDI CCNNGG 3 cut(s) 141, 752, 764
BseGI GGATG 1 cut(s) 1016
BseLI CCNNNNNNNGG 5 cut(s) 45, 185, 206, 281, 611
BseMI GCAATG 1 cut(s) 422
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 4 cut(s) 331, 626, 851, 1045
BseRI GAGGAG 4 cut(s) 50, 53, 79, 916
BseSI GKGCMC 1 cut(s) 776
BseXI GCAGC 3 cut(s) 169, 667, 964
BseYI CCCAGC 1 cut(s) 566
BshFI GGCC 3 cut(s) 140, 154, 625
BshNI GGYRCC 1 cut(s) 48
BshTI ACCGGT 1 cut(s) 199
BsiSI CCGG 4 cut(s) 155, 200, 658, 764
BslI CCNNNNNNNGG 5 cut(s) 45, 185, 206, 281, 611
BsmAI GTCTC 2 cut(s) 286, 822
BsmBI CGTCTC 1 cut(s) 822
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 3 cut(s) 140, 154, 625
Bsp119I TTCGAA 2 cut(s) 536, 686
Bsp1286I GDGCHC 1 cut(s) 776
Bsp143I GATC 1 cut(s) 748
BspACI CCGC 3 cut(s) 137, 272, 912
BspANI GGCC 3 cut(s) 140, 154, 625
BspCNI CTCAG 1 cut(s) 628
BspLI GGNNCC 1 cut(s) 50
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 1 cut(s) 743
BspT104I TTCGAA 2 cut(s) 536, 686
BspT107I GGYRCC 1 cut(s) 48
BsrDI GCAATG 1 cut(s) 422
BsrFI RCCGGY 2 cut(s) 199, 657
BsrI ACTGG 4 cut(s) 331, 626, 851, 1045
BssAI RCCGGY 2 cut(s) 199, 657
BssECI CCNNGG 3 cut(s) 141, 752, 764
BssMI GATC 1 cut(s) 748
Bst2UI CCWGG 2 cut(s) 282, 753
Bst4CI ACNGT 4 cut(s) 149, 167, 322, 631
Bst6I CTCTTC 2 cut(s) 71, 773
BstAPI GCANNNNNTGC 1 cut(s) 1064
BstBI TTCGAA 2 cut(s) 536, 686
BstC8I GCNNGC 4 cut(s) 97, 116, 187, 982
BstDEI CTNAG 2 cut(s) 561, 636
BstDSI CCRYGG 1 cut(s) 141
BstENI CCTNNNNNAGG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 1016
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 1 cut(s) 751
BstMAI GTCTC 2 cut(s) 286, 822
BstMBI GATC 1 cut(s) 748
BstMWI GCNNNNNNNGC 4 cut(s) 134, 277, 1041, 1064
BstNI CCWGG 2 cut(s) 282, 753
BstSCI CCNGG 4 cut(s) 154, 280, 751, 763
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 776
BstV1I GCAGC 3 cut(s) 169, 667, 964
BstX2I RGATCY 1 cut(s) 748
BstYI RGATCY 1 cut(s) 748
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 3 cut(s) 140, 154, 625
BtgI CCRYGG 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 911
BtsCI GGATG 1 cut(s) 1016
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 4 cut(s) 429, 627, 797, 1118
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 4 cut(s) 97, 116, 187, 982
CfoI GCGC 1 cut(s) 271
Cfr10I RCCGGY 2 cut(s) 199, 657
Cfr13I GGNCC 3 cut(s) 152, 624, 947
CseI GACGC 1 cut(s) 804
Csp6I GTAC 7 cut(s) 49, 132, 163, 226, 724, 969, 998
CspAI ACCGGT 1 cut(s) 199
CviAII CATG 2 cut(s) 231, 727
CviQI GTAC 7 cut(s) 49, 132, 163, 226, 724, 969, 998
DdeI CTNAG 2 cut(s) 561, 636
DpnI GATC 1 cut(s) 750
DpnII GATC 1 cut(s) 748
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 2 cut(s) 71, 773
EarI CTCTTC 2 cut(s) 71, 773
EciI GGCGGA 1 cut(s) 927
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 947
EcoNI CCTNNNNNAGG 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 802
EcoRII CCWGG 2 cut(s) 280, 751
EcoRV GATATC 1 cut(s) 388
Esp3I CGTCTC 1 cut(s) 822
FaeI CATG 2 cut(s) 234, 730
FatI CATG 2 cut(s) 230, 726
Fnu4HI GCNGC 5 cut(s) 138, 183, 272, 681, 978
FokI GGATG 1 cut(s) 1003
Fsp4HI GCNGC 5 cut(s) 138, 183, 272, 681, 978
FspBI CTAG 2 cut(s) 122, 1005
GlaI GCGC 1 cut(s) 270
GluI GCNGC 5 cut(s) 138, 183, 272, 681, 978
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 765
HaeIII GGCC 3 cut(s) 140, 154, 625
HapII CCGG 4 cut(s) 155, 200, 658, 764
HgaI GACGC 1 cut(s) 804
HhaI GCGC 1 cut(s) 271
Hin1II CATG 2 cut(s) 234, 730
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 1 cut(s) 546
HindII GTYRAC 1 cut(s) 546
HindIII AAGCTT 2 cut(s) 126, 1033
HinfI GANTC 6 cut(s) 193, 241, 294, 788, 863, 1087
HpaI GTTAAC 1 cut(s) 546
HpaII CCGG 4 cut(s) 155, 200, 658, 764
HphI GGTGA 4 cut(s) 6, 250, 626, 647
Hpy166II GTNNAC 4 cut(s) 14, 228, 546, 558
Hpy188I TCNGA 1 cut(s) 1086
Hpy188III TCNNGA 4 cut(s) 122, 289, 311, 379
Hpy8I GTNNAC 4 cut(s) 14, 228, 546, 558
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 4 cut(s) 226, 532, 682, 695
HpyCH4III ACNGT 4 cut(s) 149, 167, 322, 631
HpyCH4V TGCA 7 cut(s) 185, 446, 529, 646, 870, 1028, 1058
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 277, 1041, 1064
HpyF3I CTNAG 2 cut(s) 561, 636
Hsp92II CATG 2 cut(s) 234, 730
HspAI GCGC 1 cut(s) 269
KpnI GGTACC 1 cut(s) 52
KspAI GTTAAC 1 cut(s) 546
Kzo9I GATC 1 cut(s) 748
LmnI GCTCC 2 cut(s) 104, 1072
Lsp1109I GCAGC 3 cut(s) 169, 667, 964
LweI GCATC 4 cut(s) 256, 267, 916, 1025
MaeI CTAG 2 cut(s) 122, 1005
MaeIII GTNAC 4 cut(s) 238, 322, 502, 631
MalI GATC 1 cut(s) 750
MboI GATC 1 cut(s) 748
MboII GAAGA 6 cut(s) 58, 326, 790, 817, 1071, 1106
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 748
MhlI GDGCHC 1 cut(s) 776
MluCI AATT 5 cut(s) 42, 447, 802, 832, 1060
MlyI GAGTC 3 cut(s) 187, 235, 288
MmeI TCCRAC 4 cut(s) 342, 552, 1044, 1094
MseI TTAA 5 cut(s) 35, 204, 450, 479, 545
MspI CCGG 4 cut(s) 155, 200, 658, 764
MspR9I CCNGG 4 cut(s) 156, 282, 753, 765
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 2 cut(s) 282, 753
MwoI GCNNNNNNNGC 4 cut(s) 134, 277, 1041, 1064
NciI CCSGG 2 cut(s) 156, 765
NdeII GATC 1 cut(s) 748
NlaIII CATG 2 cut(s) 234, 730
NlaIV GGNNCC 1 cut(s) 50
NmeAIII GCCGAG 1 cut(s) 630
NmuCI GTSAC 3 cut(s) 238, 502, 631
NspV TTCGAA 2 cut(s) 536, 686
PctI GAATGC 1 cut(s) 877
PfeI GAWTC 3 cut(s) 788, 863, 1087
PinAI ACCGGT 1 cut(s) 199
PkrI GCNGC 5 cut(s) 139, 184, 273, 682, 979
PleI GAGTC 3 cut(s) 187, 235, 288
PpsI GAGTC 3 cut(s) 187, 235, 288
PsiI TTATAA 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 280, 751
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 2 cut(s) 280, 751
PspN4I GGNNCC 1 cut(s) 50
PspPI GGNCC 3 cut(s) 152, 624, 947
PstI CTGCAG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 748
RsaI GTAC 7 cut(s) 50, 133, 164, 227, 725, 970, 999
RsaNI GTAC 7 cut(s) 49, 132, 163, 226, 724, 969, 998
SaqAI TTAA 5 cut(s) 35, 204, 450, 479, 545
SatI GCNGC 5 cut(s) 138, 183, 272, 681, 978
Sau3AI GATC 1 cut(s) 748
Sau96I GGNCC 3 cut(s) 152, 624, 947
ScaI AGTACT 1 cut(s) 970
SchI GAGTC 3 cut(s) 187, 235, 288
ScrFI CCNGG 4 cut(s) 156, 282, 753, 765
SduI GDGCHC 1 cut(s) 776
SfaNI GCATC 4 cut(s) 256, 267, 916, 1025
SfcI CTRYAG 1 cut(s) 183
SfuI TTCGAA 2 cut(s) 536, 686
SinI GGWCC 1 cut(s) 947
Sse9I AATT 5 cut(s) 42, 447, 802, 832, 1060
SsiI CCGC 3 cut(s) 137, 272, 912
SspI AATATT 1 cut(s) 581
SspMI CTAG 2 cut(s) 122, 1005
StyD4I CCNGG 4 cut(s) 154, 280, 751, 763
TaaI ACNGT 4 cut(s) 149, 167, 322, 631
TaqI TCGA 5 cut(s) 264, 378, 516, 536, 686
TasI AATT 5 cut(s) 42, 447, 802, 832, 1060
TatI WGTACW 5 cut(s) 131, 162, 225, 723, 968
TauI GCSGC 2 cut(s) 140, 274
TfiI GAWTC 3 cut(s) 788, 863, 1087
Tru1I TTAA 5 cut(s) 35, 204, 450, 479, 545
Tru9I TTAA 5 cut(s) 35, 204, 450, 479, 545
TscAI CASTG 4 cut(s) 436, 634, 804, 1125
TseFI GTSAC 3 cut(s) 238, 502, 631
TseI GCWGC 3 cut(s) 182, 680, 977
Tsp45I GTSAC 3 cut(s) 238, 502, 631
TspDTI ATGAA 2 cut(s) 753, 956
TspGWI ACGGA 2 cut(s) 158, 476
TspRI CASTG 4 cut(s) 436, 634, 804, 1125
VpaK11BI GGWCC 1 cut(s) 947
XagI CCTNNNNNAGG 1 cut(s) 183
XapI RAATTY 2 cut(s) 802, 1060
XbaI TCTAGA 1 cut(s) 121
XcmI CCANNNNNNNNNTGG 1 cut(s) 338
XspI CTAG 2 cut(s) 122, 1005
ZrmI AGTACT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.