FvH4_1g16250

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011277
Physical Location & Seq
Reverse (-)
9319138 .. 9322719
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16250.t2

Sequence Viewer

Length: 1278 bp
ATGTTACCCTGTTCACCGCACCACCGTAGCTTACGCAGACCCTCAACCACAATTGGGTACCTCTATCTCCTCCTCTTCTCTCTCCTTTCCACAACCTCCACTTCTTGCTCCAATGGAAGCTGCCAGCTTCTAGAAGCTTGTGCTGCCGCCACGGACTGTGGCCCGGGCCTTTACTGTGGCAACTGCCCTGTTTCCGGCAAGACTCAACCGGTTTGCACCAGAGGCCAGGCTGTCATACCCACTTCTGTTGTTAATGGGTTGCCGTTCAACAAGTACACGTGGCTGGTGACTCATAACTCCTTCAGCATCGTCGACGCACCGCCTTTGCCTGGTGTCCAGAGGCTCACATTTTACAATCAGGAAGATACAGTGACTAACCAGTTGAGGAATGGAGTGAGGGGGTTGATGTTGGATATGTATGATTTCGAGAACGATGTCTGGCTTTGCCATTCATTTCGGGGGCAATGCTACAACTTCACTGCGTTTCAACCTGCAATTAATACTATGATGGAGGTGGAAGCATTTTTGAGCGAGAACCCGGCAGAGATTGTGACTATTATAATTGAGGACTATGTGCATACTACGAAAGGGTTAACAGCATTGTTTGCTAAGGCTGGGTTGGACAAATATTGGTTTCCTGTGTCCAAAATGCCAAGGAAGGGTGATGACTGGCCCACTGTGACTGAGATGGTGCAAGACAATCACCGGCTTATTGTTTTCACCTCTGATGCTACAAAAGAAGCACAGGAGGGAGTAGCTTACCAGTGGAAGTACATGGTGGAAAATGAATCTGGAGATCCTGGGGTCAAACCAGGTTCGTGCCCTAAAAGGAAAGAATCAAAGGCACTGACTTCAAAAAGTGCCTCTCTTTTCTTCCAAAATTATTTCCCAACGTATCCAGTTGAGACCGAAGCTTGCAAAGAGCATTCAACTCCACTTCAAGCAATGGTCGGAACCTGTTACAAAGCAGCTGGGAGAATGCCAAACTTTTTGGCTGTCAACTTTTACATGAGGAGTGATGGCGGAGGTGTATTTGATGCTGTTGATAGAATGAATGGACCAACACTTTGTGGGTGTAGTACCCTTGCTGCCTGCCAGGCTGGAGCACCTTTTGGATCCTGCAAGAATGTTTCTGTACCTGCTAGAAGTCCAGCAATCAACAATGCTGGAAGCTTTACTGGTTCTGTTCAGTTTGCAAATTCTGCTTCAGCGCTTAGTTCTACGAATCGTATTATTGCATTTCTATTCTATCTTCCACTGATGGCATTTTGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

46.52

Weight (kDa)

6.99

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 76 - 366 9.5e-151 PI-PLC-like catalytic domain
PI-PLC_X PF26146 114 - 338 4e-19 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67130
fragaria_vesca FvH4_1g16250 FvH4_1g16250 FvH4_1g16250
malus_domestica MD02G1174800.v1.1 MD15G1285800.v1.1
prunus_persica Prupe.7G131000_v2.0.a1
pyrus_communis pycom02g14120 pycom15g24890
rosa_chinensis RchiOBHm_Chr2g0105961
rosa_laevigata RLG00000017473
rosa_multiflora Rmu_sc0004367.1_g000025
rosa_roxburghii Rroxscaffold_2G00137520 Rroxscaffold_2G00137670
rosa_rugosa Rorug02G0132600
rosa_samantha Rh2AG182900 Rh2BG193100 Rh2CG187400 Rh2DG189400
rosa_wichuraiana Rw2G014350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
Acc36I ACCTGC 2 cut(s) 499, 1147
Acc65I GGTACC 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 57
AccI GTMKAC 1 cut(s) 312
AciI CCGC 4 cut(s) 17, 147, 320, 1023
AclWI GGATC 3 cut(s) 791, 1110, 1123
AcsI RAATTY 1 cut(s) 1198
AcuI CTGAAG 2 cut(s) 286, 1191
AcvI CACGTG 1 cut(s) 279
AdeI CACNNNGTG 1 cut(s) 1070
AfaI GTAC 5 cut(s) 59, 275, 773, 1081, 1137
AfeI AGCGCT 1 cut(s) 1212
AfiI CCNNNNNNNGG 4 cut(s) 54, 194, 329, 659
AflIII ACRYGT 1 cut(s) 276
AgeI ACCGGT 1 cut(s) 208
AgsI TTSAA 5 cut(s) 268, 488, 855, 930, 941
AjnI CCWGG 5 cut(s) 225, 328, 799, 811, 1095
AluBI AGCT 8 cut(s) 30, 120, 127, 137, 758, 914, 971, 1173
AluI AGCT 8 cut(s) 30, 120, 127, 137, 758, 914, 971, 1173
Alw21I GWGCWC 1 cut(s) 1108
Alw26I GTCTC 1 cut(s) 899
AlwI GGATC 3 cut(s) 791, 1110, 1123
Ama87I CYCGRG 1 cut(s) 163
Aor51HI AGCGCT 1 cut(s) 1212
AoxI GGCC 4 cut(s) 160, 166, 223, 671
ApeKI GCWGC 4 cut(s) 120, 143, 968, 1088
ApoI RAATTY 1 cut(s) 1198
ArsI GACNNNNNNTTYG 2 cut(s) 1050, 1082
AseI ATTAAT 1 cut(s) 498
AsiGI ACCGGT 1 cut(s) 208
Asp718I GGTACC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 1213
AspS9I GGNCC 4 cut(s) 161, 166, 672, 1058
AsuC2I CCSGG 3 cut(s) 164, 165, 539
AsuHPI GGTGA 5 cut(s) 6, 298, 674, 695, 712
AvaI CYCGRG 1 cut(s) 163
AvaII GGWCC 1 cut(s) 1058
BaeGI GKGCMC 1 cut(s) 824
BamHI GGATCC 1 cut(s) 1115
BanI GGYRCC 1 cut(s) 57
BbrPI CACGTG 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 1108
BbvI GCAGC 4 cut(s) 107, 130, 980, 1075
BccI CCATC 4 cut(s) 502, 682, 1013, 1255
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 5 cut(s) 227, 330, 801, 813, 1097
BciVI GTATCC 1 cut(s) 906
BcnI CCSGG 3 cut(s) 164, 165, 539
BcoDI GTCTC 1 cut(s) 899
BfaI CTAG 2 cut(s) 131, 1143
BfoI RGCGCY 1 cut(s) 1214
BfuAI ACCTGC 2 cut(s) 499, 1147
BfuI GTATCC 1 cut(s) 906
BglI GCCNNNNNGGC 1 cut(s) 1097
BisI GCNGC 5 cut(s) 121, 144, 147, 969, 1089
BlsI GCNGC 5 cut(s) 122, 145, 148, 970, 1090
Bme1390I CCNGG 8 cut(s) 164, 165, 227, 330, 539, 801, 813, 1097
Bme18I GGWCC 1 cut(s) 1058
BmeT110I CYCGRG 1 cut(s) 163
BmgT120I GGNCC 4 cut(s) 161, 166, 672, 1058
BmiI GGNNCC 3 cut(s) 59, 955, 1117
BmrFI CCNGG 8 cut(s) 164, 165, 227, 330, 539, 801, 813, 1097
BmsI GCATC 3 cut(s) 315, 718, 1027
BpmI CTGGAG 2 cut(s) 813, 1122
Bpu10I CCTNAGC 1 cut(s) 609
BpuMI CCSGG 3 cut(s) 164, 165, 539
BsaAI YACGTR 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 899
BsaJI CCNNGG 4 cut(s) 150, 163, 653, 800
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 194, 329, 659
Bse118I RCCGGY 2 cut(s) 208, 705
Bse1I ACTGG 5 cut(s) 379, 674, 763, 899, 1183
Bse3DI GCAATG 2 cut(s) 470, 951
BseBI CCWGG 5 cut(s) 227, 330, 801, 813, 1097
BseDI CCNNGG 4 cut(s) 150, 163, 653, 800
BseLI CCNNNNNNNGG 4 cut(s) 54, 194, 329, 659
BseMI GCAATG 2 cut(s) 470, 951
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 5 cut(s) 379, 674, 763, 899, 1183
BseRI GAGGAG 3 cut(s) 59, 62, 1027
BseSI GKGCMC 1 cut(s) 824
BseXI GCAGC 4 cut(s) 107, 130, 980, 1075
BseYI CCCAGC 2 cut(s) 614, 971
BshFI GGCC 4 cut(s) 162, 168, 225, 673
BshNI GGYRCC 1 cut(s) 57
BshTI ACCGGT 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 1108
BsiHKCI CYCGRG 1 cut(s) 163
BsiSI CCGG 5 cut(s) 164, 195, 209, 539, 706
BslI CCNNNNNNNGG 4 cut(s) 54, 194, 329, 659
BsmAI GTCTC 1 cut(s) 899
BsmI GAATGC 2 cut(s) 925, 984
BsnI GGCC 4 cut(s) 162, 168, 225, 673
Bso31I GGTCTC 1 cut(s) 899
BsoBI CYCGRG 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 824, 1108
Bsp143I GATC 2 cut(s) 796, 1115
BspACI CCGC 4 cut(s) 17, 147, 320, 1023
BspANI GGCC 4 cut(s) 162, 168, 225, 673
BspCNI CTCAG 1 cut(s) 676
BspLI GGNNCC 3 cut(s) 59, 955, 1117
BspMI ACCTGC 2 cut(s) 499, 1147
BspPI GGATC 3 cut(s) 791, 1110, 1123
BspT107I GGYRCC 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 899
BsrDI GCAATG 2 cut(s) 470, 951
BsrFI RCCGGY 2 cut(s) 208, 705
BsrI ACTGG 5 cut(s) 379, 674, 763, 899, 1183
BssAI RCCGGY 2 cut(s) 208, 705
BssECI CCNNGG 4 cut(s) 150, 163, 653, 800
BssMI GATC 2 cut(s) 796, 1115
BssT1I CCWWGG 1 cut(s) 653
Bst2UI CCWGG 5 cut(s) 227, 330, 801, 813, 1097
Bst4CI ACNGT 5 cut(s) 26, 158, 176, 370, 679
Bst6I CTCTTC 1 cut(s) 80
BstAPI GCANNNNNTGC 2 cut(s) 605, 1202
BstBAI YACGTR 1 cut(s) 279
BstC8I GCNNGC 3 cut(s) 125, 916, 1093
BstDEI CTNAG 3 cut(s) 609, 684, 1214
BstDSI CCRYGG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 1214
BstHHI GCGC 1 cut(s) 1213
BstKTI GATC 2 cut(s) 799, 1118
BstMAI GTCTC 1 cut(s) 899
BstMBI GATC 2 cut(s) 796, 1115
BstMWI GCNNNNNNNGC 5 cut(s) 143, 222, 605, 1097, 1202
BstNI CCWGG 5 cut(s) 227, 330, 801, 813, 1097
BstSCI CCNGG 8 cut(s) 162, 163, 225, 328, 537, 799, 811, 1095
BstSLI GKGCMC 1 cut(s) 824
BstV1I GCAGC 4 cut(s) 107, 130, 980, 1075
BstX2I RGATCY 2 cut(s) 796, 1115
BstYI RGATCY 2 cut(s) 796, 1115
BsuI GTATCC 1 cut(s) 906
BsuRI GGCC 4 cut(s) 162, 168, 225, 673
BtgI CCRYGG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 6 cut(s) 375, 477, 675, 770, 845, 1256
BveI ACCTGC 2 cut(s) 499, 1147
Cac8I GCNNGC 3 cut(s) 125, 916, 1093
CfoI GCGC 1 cut(s) 1213
Cfr10I RCCGGY 2 cut(s) 208, 705
Cfr13I GGNCC 4 cut(s) 161, 166, 672, 1058
Cfr9I CCCGGG 1 cut(s) 163
CseI GACGC 1 cut(s) 323
CsiI ACCWGGT 1 cut(s) 811
Csp6I GTAC 5 cut(s) 58, 274, 772, 1080, 1136
CspAI ACCGGT 1 cut(s) 208
CspCI CAANNNNNGTGG 2 cut(s) 229, 264
CviAII CATG 2 cut(s) 775, 1009
CviQI GTAC 5 cut(s) 58, 274, 772, 1080, 1136
DdeI CTNAG 3 cut(s) 609, 684, 1214
DpnI GATC 2 cut(s) 798, 1117
DpnII GATC 2 cut(s) 796, 1115
DraIII CACNNNGTG 1 cut(s) 1070
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 1038
Eco130I CCWWGG 1 cut(s) 653
Eco31I GGTCTC 1 cut(s) 899
Eco47I GGWCC 1 cut(s) 1058
Eco47III AGCGCT 1 cut(s) 1212
Eco57I CTGAAG 2 cut(s) 286, 1191
Eco72I CACGTG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 163
EcoRII CCWGG 5 cut(s) 225, 328, 799, 811, 1095
EcoT14I CCWWGG 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 2 cut(s) 778, 1012
FatI CATG 2 cut(s) 774, 1008
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 5 cut(s) 121, 144, 147, 969, 1089
Fsp4HI GCNGC 5 cut(s) 121, 144, 147, 969, 1089
FspBI CTAG 2 cut(s) 131, 1143
GlaI GCGC 1 cut(s) 1212
GluI GCNGC 5 cut(s) 121, 144, 147, 969, 1089
GsaI CCCAGC 2 cut(s) 618, 975
GsuI CTGGAG 2 cut(s) 813, 1122
HaeII RGCGCY 1 cut(s) 1214
HaeIII GGCC 4 cut(s) 162, 168, 225, 673
HapII CCGG 5 cut(s) 164, 195, 209, 539, 706
HgaI GACGC 1 cut(s) 323
HhaI GCGC 1 cut(s) 1213
Hin1II CATG 2 cut(s) 778, 1012
Hin6I GCGC 1 cut(s) 1211
HinP1I GCGC 1 cut(s) 1211
HincII GTYRAC 3 cut(s) 313, 594, 1000
HindII GTYRAC 3 cut(s) 313, 594, 1000
HindIII AAGCTT 3 cut(s) 135, 912, 1171
HinfI GANTC 5 cut(s) 202, 289, 788, 836, 1225
HpaI GTTAAC 1 cut(s) 594
HpaII CCGG 5 cut(s) 164, 195, 209, 539, 706
HphI GGTGA 5 cut(s) 6, 298, 674, 695, 712
Hpy166II GTNNAC 5 cut(s) 14, 276, 313, 594, 1000
Hpy188I TCNGA 2 cut(s) 727, 953
Hpy188III TCNNGA 5 cut(s) 131, 337, 359, 427, 792
Hpy8I GTNNAC 5 cut(s) 14, 276, 313, 594, 1000
Hpy99I CGWCG 2 cut(s) 314, 317
HpyAV CCTTC 2 cut(s) 310, 652
HpyCH4III ACNGT 5 cut(s) 26, 158, 176, 370, 679
HpyCH4IV ACGT 2 cut(s) 278, 893
HpyCH4V TGCA 8 cut(s) 216, 494, 577, 694, 918, 1122, 1196, 1238
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 222, 605, 1097, 1202
HpyF3I CTNAG 3 cut(s) 609, 684, 1214
HpySE526I ACGT 2 cut(s) 278, 893
Hsp92II CATG 2 cut(s) 778, 1012
HspAI GCGC 1 cut(s) 1211
KpnI GGTACC 1 cut(s) 61
KspAI GTTAAC 1 cut(s) 594
Kzo9I GATC 2 cut(s) 796, 1115
LmnI GCTCC 2 cut(s) 113, 1103
Lsp1109I GCAGC 4 cut(s) 107, 130, 980, 1075
LweI GCATC 3 cut(s) 315, 718, 1027
MabI ACCWGGT 1 cut(s) 811
MaeI CTAG 2 cut(s) 131, 1143
MaeII ACGT 2 cut(s) 278, 893
MaeIII GTNAC 6 cut(s) 3, 286, 370, 550, 679, 959
MalI GATC 2 cut(s) 798, 1117
MboI GATC 2 cut(s) 796, 1115
MboII GAAGA 4 cut(s) 67, 374, 865, 1244
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 2 cut(s) 796, 1115
MhlI GDGCHC 2 cut(s) 824, 1108
MluCI AATT 5 cut(s) 51, 495, 561, 880, 1198
MlyI GAGTC 2 cut(s) 196, 283
MmeI TCCRAC 3 cut(s) 390, 600, 931
MseI TTAA 3 cut(s) 252, 498, 593
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 5 cut(s) 164, 195, 209, 539, 706
MspR9I CCNGG 8 cut(s) 164, 165, 227, 330, 539, 801, 813, 1097
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 2 cut(s) 925, 984
MvaI CCWGG 5 cut(s) 227, 330, 801, 813, 1097
MwoI GCNNNNNNNGC 5 cut(s) 143, 222, 605, 1097, 1202
NciI CCSGG 3 cut(s) 164, 165, 539
NdeII GATC 2 cut(s) 796, 1115
NlaIII CATG 2 cut(s) 778, 1012
NlaIV GGNNCC 3 cut(s) 59, 955, 1117
NmuCI GTSAC 4 cut(s) 286, 370, 550, 679
PctI GAATGC 2 cut(s) 925, 984
PfeI GAWTC 3 cut(s) 788, 836, 1225
PinAI ACCGGT 1 cut(s) 208
PkrI GCNGC 5 cut(s) 122, 145, 148, 970, 1090
PleI GAGTC 2 cut(s) 196, 283
PmaCI CACGTG 1 cut(s) 279
PmlI CACGTG 1 cut(s) 279
PpsI GAGTC 2 cut(s) 196, 283
Ppu21I YACGTR 1 cut(s) 279
PshBI ATTAAT 1 cut(s) 498
PsiI TTATAA 1 cut(s) 560
Psp6I CCWGG 5 cut(s) 225, 328, 799, 811, 1095
PspCI CACGTG 1 cut(s) 279
PspFI CCCAGC 2 cut(s) 614, 971
PspGI CCWGG 5 cut(s) 225, 328, 799, 811, 1095
PspN4I GGNNCC 3 cut(s) 59, 955, 1117
PspPI GGNCC 4 cut(s) 161, 166, 672, 1058
PsuI RGATCY 2 cut(s) 796, 1115
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 5 cut(s) 59, 275, 773, 1081, 1137
RsaNI GTAC 5 cut(s) 58, 274, 772, 1080, 1136
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 3 cut(s) 252, 498, 593
SatI GCNGC 5 cut(s) 121, 144, 147, 969, 1089
Sau3AI GATC 2 cut(s) 796, 1115
Sau96I GGNCC 4 cut(s) 161, 166, 672, 1058
SchI GAGTC 2 cut(s) 196, 283
ScrFI CCNGG 8 cut(s) 164, 165, 227, 330, 539, 801, 813, 1097
SduI GDGCHC 2 cut(s) 824, 1108
SexAI ACCWGGT 1 cut(s) 811
SfaNI GCATC 3 cut(s) 315, 718, 1027
SgrDI CGTCGACG 1 cut(s) 311
SinI GGWCC 1 cut(s) 1058
SmaI CCCGGG 1 cut(s) 165
SrfI GCCCGGGC 1 cut(s) 165
Sse9I AATT 5 cut(s) 51, 495, 561, 880, 1198
SsiI CCGC 4 cut(s) 17, 147, 320, 1023
SspI AATATT 1 cut(s) 629
SspMI CTAG 2 cut(s) 131, 1143
StyD4I CCNGG 8 cut(s) 162, 163, 225, 328, 537, 799, 811, 1095
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 5 cut(s) 26, 158, 176, 370, 679
TaiI ACGT 2 cut(s) 281, 896
TaqI TCGA 2 cut(s) 312, 426
TaqII GACCGA 1 cut(s) 923
TasI AATT 5 cut(s) 51, 495, 561, 880, 1198
TatI WGTACW 2 cut(s) 273, 771
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 3 cut(s) 788, 836, 1225
Tru1I TTAA 3 cut(s) 252, 498, 593
Tru9I TTAA 3 cut(s) 252, 498, 593
TscAI CASTG 6 cut(s) 375, 484, 682, 770, 852, 1263
TseFI GTSAC 4 cut(s) 286, 370, 550, 679
TseI GCWGC 4 cut(s) 120, 143, 968, 1088
Tsp45I GTSAC 4 cut(s) 286, 370, 550, 679
TspDTI ATGAA 3 cut(s) 441, 801, 1067
TspGWI ACGGA 1 cut(s) 167
TspMI CCCGGG 1 cut(s) 163
TspRI CASTG 6 cut(s) 375, 484, 682, 770, 852, 1263
VpaK11BI GGWCC 1 cut(s) 1058
VspI ATTAAT 1 cut(s) 498
XapI RAATTY 1 cut(s) 1198
XbaI TCTAGA 1 cut(s) 130
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
XmaI CCCGGG 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 2 cut(s) 131, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.