pycom02g14120

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011277
Physical Location & Seq
Reverse (-)
10706241 .. 10708498
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g14120.3

Sequence Viewer

Length: 1275 bp
ATGCCACCGTGCTCGGCGGAACGCGGCGGCGTATCCAGAGCCTCGTCAACTGTCGTCCTCTTCCTGCTACTCTTCTCAGTCCTGGCCACCAGTTCCACTGCTTGCTCCAACGGCAATTGCCAGGTTCTAGATGCTTGTGCGGCGGCGACGGACTGCGGGCCGGGTCTTTACTGTGGCAACTGCCCTGCTTCGGGTAAGAATCAGCCCGTTTGCACCAGAGGCCAAGCAATCGTACCCACTTCAATTATTAATGGGTTGCCATTCAACAAGTACACATGGTTGGCGACTCATAATTCTTTCAGCATCGTTGATGCACCTCCCGTGGCTGGTGTTCAAAGACTCACATTTTACAACCAAGAGGACACTGTTACTAACCAGTTGAGAAATGGAGTGAGAGGGCTGATGTTGGATATGTACGATTTTGAGAATGATATCTGGCTTTGTCATTCCTTTAAGGGGCAATGTTTCAACTTCACTGCATTTCAACCTGCAATTAACACTTTGACGGAAGTGGAAACATTTTTAAGTCAGAACCCGACAGAGATTGTGACCATTATAATTGAGGACTATGTGAAAACTCCAAAAGGGTTAACGACATTGTTTACCAATGCTGGGTTAAATAAGTATTGGTTTCCTGTAGCGAAAATGCCAAGAAAGGGTGAAGATTGGCCCACTGTTACTGAAATGGTGCAAGAGAATCATCGGCTTCTTGTTTTTACGTCTGATGCTTCAAAGGAAGCACAGGAAGGAGTAGCTTACCAGTGGAAATACATGGTGGAAAACGAAGCTGGAGATCCTGGGGTAAAACCAGGCTCATGCCCGAAGAGAAAAGAATCAAAGCCTCTGAATTCAAAAAGTGCATCTCTTTTCTTTCAAAATTATTTCCCAACATATCCTGTTGAAGCTGAAGCTTGCGAAGAGCATTCAATTCCACTGGAGCAGATGGTCAGTACCTGTTATAAAGCTGCAGGGGTGATGCCGAATTTTTTGGCAGTCAACTTTTACATGAGGAGTGATGGAGAAGGTGTGTTTGATGCTGTTGATAGAATGAATGGTCCGGTGCTTTGCGGGTGTAGTACTGTCAGTGCCTGCCAGGCTGGAGCACCTTCTGGATCTTGCAAGAATATTTCTGTACCAACTAGAAGTCCATTGACCAACAATGCAGGAAGCTTTACTGGCTCTGTTCAGTTTTCAAGGTCTGCTTCAACAGTCTATTCTCCAAATCGCTTGGTTGTCTTCTTGTTTTATCTTCCGTTGATGGTATTTTGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

425

Amino Acids

46.3

Weight (kDa)

5.7

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67130
fragaria_vesca FvH4_1g16250 FvH4_1g16250 FvH4_1g16250
malus_domestica MD02G1174800.v1.1 MD15G1285800.v1.1
prunus_persica Prupe.7G131000_v2.0.a1
pyrus_communis pycom02g14120 pycom15g24890
rosa_chinensis RchiOBHm_Chr2g0105961
rosa_laevigata RLG00000017473
rosa_multiflora Rmu_sc0004367.1_g000025
rosa_roxburghii Rroxscaffold_2G00137520 Rroxscaffold_2G00137670
rosa_rugosa Rorug02G0132600
rosa_samantha Rh2AG182900 Rh2BG193100 Rh2CG187400 Rh2DG189400
rosa_wichuraiana Rw2G014350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 557, 960
Acc36I ACCTGC 1 cut(s) 496
AccII CGCG 1 cut(s) 24
AciI CCGC 7 cut(s) 17, 24, 27, 140, 143, 156, 1068
AclWI GGATC 2 cut(s) 788, 1120
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 2 cut(s) 847, 982
AcuI CTGAAG 1 cut(s) 927
AfaI GTAC 6 cut(s) 234, 272, 416, 952, 1078, 1134
AfiI CCNNNNNNNGG 6 cut(s) 190, 191, 326, 456, 612, 656
AjnI CCWGG 5 cut(s) 81, 120, 796, 808, 1092
AluBI AGCT 6 cut(s) 755, 788, 905, 911, 965, 1170
AluI AGCT 6 cut(s) 755, 788, 905, 911, 965, 1170
Alw21I GWGCWC 2 cut(s) 14, 1105
AlwI GGATC 2 cut(s) 788, 1120
AlwNI CAGNNNCTG 2 cut(s) 954, 1089
AoxI GGCC 4 cut(s) 84, 158, 220, 668
ApeKI GCWGC 1 cut(s) 965
ApoI RAATTY 2 cut(s) 847, 982
AseI ATTAAT 1 cut(s) 249
AspS9I GGNCC 3 cut(s) 158, 669, 1055
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 2 cut(s) 671, 985
AvaII GGWCC 1 cut(s) 1055
BalI TGGCCA 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 1228
Bbv12I GWGCWC 2 cut(s) 14, 1105
BbvI GCAGC 1 cut(s) 952
BccI CCATC 3 cut(s) 937, 1010, 1252
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 5 cut(s) 83, 122, 798, 810, 1094
BciVI GTATCC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 162
BfaI CTAG 2 cut(s) 128, 1140
BfmI CTRYAG 2 cut(s) 636, 966
BfuAI ACCTGC 1 cut(s) 496
BfuI GTATCC 1 cut(s) 43
BglI GCCNNNNNGGC 1 cut(s) 1094
BisI GCNGC 5 cut(s) 25, 28, 141, 144, 966
BlsI GCNGC 5 cut(s) 26, 29, 142, 145, 967
BmcAI AGTACT 1 cut(s) 1078
Bme1390I CCNGG 6 cut(s) 83, 122, 162, 798, 810, 1094
Bme18I GGWCC 1 cut(s) 1055
BmgT120I GGNCC 3 cut(s) 158, 669, 1055
BmrFI CCNGG 6 cut(s) 83, 122, 162, 798, 810, 1094
BmsI GCATC 7 cut(s) 121, 301, 312, 715, 869, 966, 1024
BpiI GAAGAC 1 cut(s) 1228
BpmI CTGGAG 3 cut(s) 810, 956, 1119
BpuMI CCSGG 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 321, 797
BsaWI WCCGGW 1 cut(s) 1057
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 191, 326, 456, 612, 656
Bse1I ACTGG 5 cut(s) 90, 376, 760, 939, 1180
Bse3DI GCAATG 1 cut(s) 467
BseBI CCWGG 5 cut(s) 83, 122, 798, 810, 1094
BseDI CCNNGG 2 cut(s) 321, 797
BseLI CCNNNNNNNGG 6 cut(s) 190, 191, 326, 456, 612, 656
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 5 cut(s) 90, 376, 760, 939, 1180
BseRI GAGGAG 1 cut(s) 1024
BseXI GCAGC 1 cut(s) 952
BseYI CCCAGC 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 4 cut(s) 86, 160, 222, 670
BsiHKAI GWGCWC 2 cut(s) 14, 1105
BsiSI CCGG 2 cut(s) 161, 1058
BslI CCNNNNNNNGG 6 cut(s) 190, 191, 326, 456, 612, 656
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 4 cut(s) 86, 160, 222, 670
Bsp1286I GDGCHC 2 cut(s) 14, 1105
Bsp143I GATC 2 cut(s) 793, 1112
BspACI CCGC 7 cut(s) 17, 24, 27, 140, 143, 156, 1068
BspANI GGCC 4 cut(s) 86, 160, 222, 670
BspCNI CTCAG 1 cut(s) 89
BspFNI CGCG 1 cut(s) 24
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 496
BspPI GGATC 2 cut(s) 788, 1120
BspQI GCTCTTC 1 cut(s) 912
BsrDI GCAATG 1 cut(s) 467
BsrI ACTGG 5 cut(s) 90, 376, 760, 939, 1180
BssECI CCNNGG 2 cut(s) 321, 797
BssMI GATC 2 cut(s) 793, 1112
Bst2UI CCWGG 5 cut(s) 83, 122, 798, 810, 1094
Bst4CI ACNGT 7 cut(s) 9, 52, 173, 367, 676, 1081, 1210
Bst6I CTCTTC 4 cut(s) 65, 77, 818, 912
BstC8I GCNNGC 4 cut(s) 103, 158, 913, 1090
BstDEI CTNAG 1 cut(s) 76
BstDSI CCRYGG 1 cut(s) 321
BstFNI CGCG 1 cut(s) 24
BstKTI GATC 2 cut(s) 796, 1115
BstMBI GATC 2 cut(s) 793, 1112
BstMWI GCNNNNNNNGC 5 cut(s) 111, 140, 219, 1094, 1176
BstNI CCWGG 5 cut(s) 83, 122, 798, 810, 1094
BstSCI CCNGG 6 cut(s) 81, 120, 160, 796, 808, 1092
BstSFI CTRYAG 2 cut(s) 636, 966
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 952
BstV2I GAAGAC 1 cut(s) 1228
BstX2I RGATCY 2 cut(s) 793, 1112
BstYI RGATCY 2 cut(s) 793, 1112
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 4 cut(s) 86, 160, 222, 670
BtgI CCRYGG 1 cut(s) 321
BtsI GCAGTG 2 cut(s) 96, 474
BtsIMutI CAGTG 7 cut(s) 96, 363, 474, 672, 767, 932, 1090
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 4 cut(s) 103, 158, 913, 1090
CaiI CAGNNNCTG 2 cut(s) 954, 1089
Cfr13I GGNCC 3 cut(s) 158, 669, 1055
Csp6I GTAC 6 cut(s) 233, 271, 415, 951, 1077, 1133
CviAII CATG 4 cut(s) 276, 772, 816, 1006
CviQI GTAC 6 cut(s) 233, 271, 415, 951, 1077, 1133
DdeI CTNAG 1 cut(s) 76
DpnI GATC 2 cut(s) 795, 1114
DpnII GATC 2 cut(s) 793, 1112
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 4 cut(s) 65, 77, 818, 912
EarI CTCTTC 4 cut(s) 65, 77, 818, 912
EciI GGCGGA 1 cut(s) 32
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 1055
Eco57I CTGAAG 1 cut(s) 927
EcoRI GAATTC 1 cut(s) 847
EcoRII CCWGG 5 cut(s) 81, 120, 796, 808, 1092
EcoRV GATATC 1 cut(s) 433
FaeI CATG 4 cut(s) 279, 775, 819, 1009
FalI AAGNNNNNCTT 2 cut(s) 1186, 1218
FatI CATG 4 cut(s) 275, 771, 815, 1005
FauI CCCGC 2 cut(s) 149, 1061
Fnu4HI GCNGC 5 cut(s) 25, 28, 141, 144, 966
Fsp4HI GCNGC 5 cut(s) 25, 28, 141, 144, 966
FspBI CTAG 2 cut(s) 128, 1140
GluI GCNGC 5 cut(s) 25, 28, 141, 144, 966
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 3 cut(s) 810, 956, 1119
HaeIII GGCC 4 cut(s) 86, 160, 222, 670
HapII CCGG 2 cut(s) 161, 1058
Hin1II CATG 4 cut(s) 279, 775, 819, 1009
HincII GTYRAC 3 cut(s) 48, 591, 997
HindII GTYRAC 3 cut(s) 48, 591, 997
HindIII AAGCTT 2 cut(s) 909, 1168
HinfI GANTC 5 cut(s) 199, 286, 339, 697, 833
HpaI GTTAAC 1 cut(s) 591
HpaII CCGG 2 cut(s) 161, 1058
HphI GGTGA 2 cut(s) 671, 985
Hpy166II GTNNAC 5 cut(s) 48, 273, 591, 603, 997
Hpy188I TCNGA 3 cut(s) 531, 724, 846
Hpy188III TCNNGA 3 cut(s) 36, 128, 1110
Hpy8I GTNNAC 5 cut(s) 48, 273, 591, 603, 997
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 3 cut(s) 740, 1016, 1116
HpyCH4III ACNGT 7 cut(s) 9, 52, 173, 367, 676, 1081, 1210
HpyCH4IV ACGT 1 cut(s) 719
HpyCH4V TGCA 9 cut(s) 213, 314, 479, 491, 691, 860, 968, 1119, 1163
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 140, 219, 1094, 1176
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 1 cut(s) 719
Hsp92II CATG 4 cut(s) 279, 775, 819, 1009
KspAI GTTAAC 1 cut(s) 591
Kzo9I GATC 2 cut(s) 793, 1112
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 3 cut(s) 110, 937, 1100
Lsp1109I GCAGC 1 cut(s) 952
LweI GCATC 7 cut(s) 121, 301, 312, 715, 869, 966, 1024
MaeI CTAG 2 cut(s) 128, 1140
MaeII ACGT 1 cut(s) 719
MaeIII GTNAC 3 cut(s) 367, 547, 676
MalI GATC 2 cut(s) 795, 1114
MboI GATC 2 cut(s) 793, 1112
MboII GAAGA 7 cut(s) 52, 64, 674, 835, 929, 1228, 1241
MfeI CAATTG 1 cut(s) 115
MflI RGATCY 2 cut(s) 793, 1112
MhlI GDGCHC 2 cut(s) 14, 1105
MlsI TGGCCA 1 cut(s) 86
MluCI AATT 9 cut(s) 115, 243, 292, 492, 558, 847, 877, 927, 982
MluNI TGGCCA 1 cut(s) 86
MlyI GAGTC 2 cut(s) 280, 333
MmeI TCCRAC 2 cut(s) 132, 387
MnlI CCTC 9 cut(s) 52, 68, 212, 327, 352, 389, 556, 852, 1002
Mox20I TGGCCA 1 cut(s) 86
MscI TGGCCA 1 cut(s) 86
MseI TTAA 6 cut(s) 249, 453, 495, 524, 590, 617
Msp20I TGGCCA 1 cut(s) 86
MspI CCGG 2 cut(s) 161, 1058
MspR9I CCNGG 6 cut(s) 83, 122, 162, 798, 810, 1094
MunI CAATTG 1 cut(s) 115
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 5 cut(s) 83, 122, 798, 810, 1094
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 5 cut(s) 111, 140, 219, 1094, 1176
NciI CCSGG 1 cut(s) 162
NdeII GATC 2 cut(s) 793, 1112
NlaIII CATG 4 cut(s) 279, 775, 819, 1009
NmuCI GTSAC 1 cut(s) 547
PciSI GCTCTTC 1 cut(s) 912
PctI GAATGC 1 cut(s) 922
PfeI GAWTC 3 cut(s) 199, 697, 833
PkrI GCNGC 5 cut(s) 26, 29, 142, 145, 967
PleI GAGTC 2 cut(s) 280, 333
PpsI GAGTC 2 cut(s) 280, 333
PshBI ATTAAT 1 cut(s) 249
PsiI TTATAA 2 cut(s) 557, 960
Psp6I CCWGG 5 cut(s) 81, 120, 796, 808, 1092
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 5 cut(s) 81, 120, 796, 808, 1092
PspPI GGNCC 3 cut(s) 158, 669, 1055
PstI CTGCAG 1 cut(s) 970
PstNI CAGNNNCTG 2 cut(s) 954, 1089
PsuI RGATCY 2 cut(s) 793, 1112
RsaI GTAC 6 cut(s) 234, 272, 416, 952, 1078, 1134
RsaNI GTAC 6 cut(s) 233, 271, 415, 951, 1077, 1133
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 6 cut(s) 249, 453, 495, 524, 590, 617
SatI GCNGC 5 cut(s) 25, 28, 141, 144, 966
Sau3AI GATC 2 cut(s) 793, 1112
Sau96I GGNCC 3 cut(s) 158, 669, 1055
ScaI AGTACT 1 cut(s) 1078
SchI GAGTC 2 cut(s) 280, 333
ScrFI CCNGG 6 cut(s) 83, 122, 162, 798, 810, 1094
SduI GDGCHC 2 cut(s) 14, 1105
SfaNI GCATC 7 cut(s) 121, 301, 312, 715, 869, 966, 1024
SfcI CTRYAG 2 cut(s) 636, 966
SinI GGWCC 1 cut(s) 1055
Sse9I AATT 9 cut(s) 115, 243, 292, 492, 558, 847, 877, 927, 982
SsiI CCGC 7 cut(s) 17, 24, 27, 140, 143, 156, 1068
SspI AATATT 1 cut(s) 1126
SspMI CTAG 2 cut(s) 128, 1140
StyD4I CCNGG 6 cut(s) 81, 120, 160, 796, 808, 1092
TaaI ACNGT 7 cut(s) 9, 52, 173, 367, 676, 1081, 1210
TaiI ACGT 1 cut(s) 722
TasI AATT 9 cut(s) 115, 243, 292, 492, 558, 847, 877, 927, 982
TatI WGTACW 2 cut(s) 270, 1076
TauI GCSGC 4 cut(s) 27, 30, 143, 146
TfiI GAWTC 3 cut(s) 199, 697, 833
Tru1I TTAA 6 cut(s) 249, 453, 495, 524, 590, 617
Tru9I TTAA 6 cut(s) 249, 453, 495, 524, 590, 617
TscAI CASTG 7 cut(s) 103, 370, 481, 679, 767, 939, 1090
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 965
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 1 cut(s) 1064
TspGWI ACGGA 3 cut(s) 164, 521, 1242
TspRI CASTG 7 cut(s) 103, 370, 481, 679, 767, 939, 1090
VpaK11BI GGWCC 1 cut(s) 1055
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 2 cut(s) 847, 982
XbaI TCTAGA 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XspI CTAG 2 cut(s) 128, 1140
ZrmI AGTACT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.