AT5G67270

Microtubule-associated protein RP EB family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015182
Physical Location & Seq
Reverse (-)
26840011 .. 26842104
2094 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67270.1

Sequence Viewer

Length: 990 bp
ATGGCTACGAACATTGGGATGATGGATTCTGCATATTTCGTCGGGAGATCGGAGATTCTGGCTTGGATTAACTCTACTCTCCAACTCAATCTCTCCAAAGTCGAAGAGGCTTGTTCAGGCGCTGTTCATTGTCAGCTGATGGATTCGGTGCATCCTGGGACTGTTCCGATGCACAAGGTTAACTTTGATGCGAAGAGTGAATATGAGATGATTCAGAACTATAAGGTTCTTCAGGATGTGTTTAATAAACTCAAGATCACTAAGCACATCGAAGTGAGCAAACTCGTTAAGGGAAGGCCATTGGATAACTTGGAGTTTATGCAGTGGATGAAAAAGTACTGTGATTCTGTTAATGGAGGCCAGCATAATTACCATGCACTTGAGAGAAGGGAAGCCAGCAAAGGAGGTAAAGAAGCAACGAAGAGAGCTGCAGCTACACAACAATCAGGCAAGAGTTCGTCTTCTTCAGCTCCACCTCGACCTTCATCTTCAAATGGAACCCGTAAGCATGAGCCACAATCCAACAACACCGGTACTCACCATTCTTCAACTGGGAATCACCACCACTCTTCAAAACCATCAGCTAAACAATCTAAACCAGTGCCTGCTTATGATGAAAAGATCACAGAACTGAAACTTTACATTGACAGCTTAGAGAAAGAGAGAGATTTCTATTTCTCTAAACTCAGAGATGTAGAGATTCTCTGTCAAAACCCAGACACAGAGCACTTACCTCTTGTTGGTTCCATAAAAAGAATCCTATACGCAGCAGATGGAGAAGACGTTGGAGCAGCAGAGACACAGACTTTGAGTCCCATTGCTGAAGGATCAGAAGAGAGAAGGAACAGTGTAACAGAGTCGCAGAAGAGGAAGCTCATAGTGAATCTCGACGTAGATGTTGCAGCGATTACAACACTCTCTCCAAGACAACGCCTTTCTGATGCTTCTGATGTCAAATGCAGTGGTTCATCTCCTCTCTTGACCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005819 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030312 GO:0030496 GO:0030865 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CH EB1

Protein Analysis

329

Amino Acids

36.39

Weight (kDa)

8.12

Isoelectric Point (pI)

56.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 16 - 107 1.4e-10 Calponin homology (CH) domain
EB1 PF03271 218 - 256 8.1e-17 EB1-like C-terminal motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67270
fragaria_vesca FvH4_1g16740
malus_domestica MD02G1178500.v1.1 MD15G1288400.v1.1
prunus_persica Prupe.6G229300_v2.0.a1
pyrus_communis pycom02g14460
rosa_chinensis RchiOBHm_Chr2g0106641
rosa_laevigata RLG00000017525
rosa_multiflora Rmu_sc0006221.1_g000003
rosa_roxburghii Rroxscaffold_2G00136930
rosa_rugosa Rorug02G0137000
rosa_samantha Rh2AG188400 Rh2BG199400 Rh2CG193400 Rh2DG194600
rosa_wichuraiana Rw2G014790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 835
AcuI CTGAAG 3 cut(s) 215, 450, 843
AfaI GTAC 2 cut(s) 338, 535
AfiI CCNNNNNNNGG 1 cut(s) 740
AgeI ACCGGT 1 cut(s) 530
AgsI TTSAA 3 cut(s) 492, 549, 573
AhdI GACNNNNNGTC 1 cut(s) 810
AjnI CCWGG 1 cut(s) 154
AluBI AGCT 7 cut(s) 136, 428, 434, 470, 584, 651, 874
AluI AGCT 7 cut(s) 136, 428, 434, 470, 584, 651, 874
Alw21I GWGCWC 1 cut(s) 729
Alw26I GTCTC 1 cut(s) 791
AlwI GGATC 1 cut(s) 835
AlwNI CAGNNNCTG 2 cut(s) 122, 605
AoxI GGCC 2 cut(s) 296, 358
ApeKI GCWGC 5 cut(s) 428, 431, 767, 791, 902
ArsI GACNNNNNNTTYG 2 cut(s) 790, 822
AsiGI ACCGGT 1 cut(s) 530
AspLEI GCGC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 530, 551
BbsI GAAGAC 2 cut(s) 453, 786
Bbv12I GWGCWC 1 cut(s) 729
BbvI GCAGC 5 cut(s) 415, 443, 779, 803, 914
BccI CCATC 4 cut(s) 16, 133, 586, 767
BciT130I CCWGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 791
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 123
BisI GCNGC 5 cut(s) 429, 432, 768, 792, 903
BlsI GCNGC 5 cut(s) 430, 433, 769, 793, 904
BmcAI AGTACT 1 cut(s) 338
Bme1390I CCNGG 1 cut(s) 156
BmeRI GACNNNNNGTC 1 cut(s) 810
BmiI GGNNCC 2 cut(s) 499, 745
BmrFI CCNGG 1 cut(s) 156
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 4 cut(s) 159, 160, 178, 931
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 2 cut(s) 453, 786
BpuEI CTTGAG 2 cut(s) 236, 401
BsaJI CCNNGG 1 cut(s) 155
BsaWI WCCGGW 1 cut(s) 530
BsaXI ACNNNNNCTCC 4 cut(s) 768, 798, 904, 934
Bsc4I CCNNNNNNNGG 1 cut(s) 740
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 2 cut(s) 556, 599
Bse3DI GCAATG 1 cut(s) 816
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 4 cut(s) 24, 151, 241, 333
BseLI CCNNNNNNNGG 1 cut(s) 740
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 1 cut(s) 700
BseNI ACTGG 2 cut(s) 556, 599
BseRI GAGGAG 1 cut(s) 963
BseXI GCAGC 5 cut(s) 415, 443, 779, 803, 914
BshFI GGCC 2 cut(s) 298, 360
BshTI ACCGGT 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 729
BsiSI CCGG 1 cut(s) 531
BslFI GGGAC 2 cut(s) 172, 798
BslI CCNNNNNNNGG 1 cut(s) 740
BsmAI GTCTC 1 cut(s) 791
BsmFI GGGAC 2 cut(s) 172, 798
BsnI GGCC 2 cut(s) 298, 360
Bsp1286I GDGCHC 1 cut(s) 729
Bsp143I GATC 4 cut(s) 47, 255, 621, 827
BspANI GGCC 2 cut(s) 298, 360
BspCNI CTCAG 1 cut(s) 699
BspLI GGNNCC 2 cut(s) 499, 745
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 1 cut(s) 835
BsrDI GCAATG 1 cut(s) 816
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 2 cut(s) 556, 599
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 4 cut(s) 47, 255, 621, 827
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 3 cut(s) 163, 341, 848
Bst6I CTCTTC 6 cut(s) 99, 188, 416, 574, 828, 860
BstC8I GCNNGC 3 cut(s) 362, 397, 606
BstDEI CTNAG 3 cut(s) 261, 652, 686
BstF5I GGATG 4 cut(s) 24, 151, 241, 333
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 4 cut(s) 50, 258, 624, 830
BstMAI GTCTC 1 cut(s) 791
BstMBI GATC 4 cut(s) 47, 255, 621, 827
BstNI CCWGG 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 154
BstSFI CTRYAG 1 cut(s) 429
BstV1I GCAGC 5 cut(s) 415, 443, 779, 803, 914
BstV2I GAAGAC 2 cut(s) 453, 786
BsuRI GGCC 2 cut(s) 298, 360
BtsCI GGATG 4 cut(s) 24, 151, 241, 333
BtsI GCAGTG 2 cut(s) 329, 967
BtsIMutI CAGTG 4 cut(s) 329, 606, 853, 967
Cac8I GCNNGC 3 cut(s) 362, 397, 606
CaiI CAGNNNCTG 2 cut(s) 122, 605
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 530
Csp6I GTAC 2 cut(s) 337, 534
CspAI ACCGGT 1 cut(s) 530
CspCI CAANNNNNGTGG 2 cut(s) 943, 978
CviAII CATG 2 cut(s) 374, 509
CviQI GTAC 2 cut(s) 337, 534
DdeI CTNAG 3 cut(s) 261, 652, 686
DpnI GATC 4 cut(s) 49, 257, 623, 829
DpnII GATC 4 cut(s) 47, 255, 621, 827
DriI GACNNNNNGTC 1 cut(s) 810
Eam1104I CTCTTC 6 cut(s) 99, 188, 416, 574, 828, 860
Eam1105I GACNNNNNGTC 1 cut(s) 810
EarI CTCTTC 6 cut(s) 99, 188, 416, 574, 828, 860
Eco57I CTGAAG 3 cut(s) 215, 450, 843
EcoRII CCWGG 1 cut(s) 154
FaeI CATG 2 cut(s) 377, 512
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FaqI GGGAC 2 cut(s) 172, 798
FatI CATG 2 cut(s) 373, 508
Fnu4HI GCNGC 5 cut(s) 429, 432, 768, 792, 903
FokI GGATG 4 cut(s) 31, 138, 248, 340
Fsp4HI GCNGC 5 cut(s) 429, 432, 768, 792, 903
GlaI GCGC 1 cut(s) 121
GluI GCNGC 5 cut(s) 429, 432, 768, 792, 903
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 2 cut(s) 298, 360
HapII CCGG 1 cut(s) 531
HhaI GCGC 1 cut(s) 122
Hin1II CATG 2 cut(s) 377, 512
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HpaI GTTAAC 1 cut(s) 181
HpaII CCGG 1 cut(s) 531
HphI GGTGA 2 cut(s) 530, 551
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 7 cut(s) 52, 168, 216, 689, 832, 940, 949
Hpy188III TCNNGA 5 cut(s) 43, 233, 253, 887, 979
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 2 cut(s) 44, 893
HpyAV CCTTC 5 cut(s) 288, 381, 492, 818, 834
HpyCH4III ACNGT 3 cut(s) 163, 341, 848
HpyCH4IV ACGT 2 cut(s) 783, 891
HpyCH4V TGCA 8 cut(s) 32, 151, 172, 322, 377, 431, 902, 960
HpyF3I CTNAG 3 cut(s) 261, 652, 686
HpySE526I ACGT 2 cut(s) 783, 891
Hsp92II CATG 2 cut(s) 377, 512
HspAI GCGC 1 cut(s) 120
KspAI GTTAAC 1 cut(s) 181
Kzo9I GATC 4 cut(s) 47, 255, 621, 827
LmnI GCTCC 2 cut(s) 475, 788
Lsp1109I GCAGC 5 cut(s) 415, 443, 779, 803, 914
LweI GCATC 4 cut(s) 159, 160, 178, 931
MaeII ACGT 2 cut(s) 783, 891
MaeIII GTNAC 1 cut(s) 850
MalI GATC 4 cut(s) 49, 257, 623, 829
MboI GATC 4 cut(s) 47, 255, 621, 827
MhlI GDGCHC 1 cut(s) 729
MluCI AATT 1 cut(s) 367
MlyI GAGTC 2 cut(s) 820, 866
MmeI TCCRAC 3 cut(s) 106, 546, 766
MnlI CCTC 7 cut(s) 100, 350, 398, 486, 744, 861, 984
MseI TTAA 5 cut(s) 69, 180, 243, 288, 351
MslI CAYNNNNRTG 2 cut(s) 17, 272
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 1 cut(s) 531
MspR9I CCNGG 1 cut(s) 156
MvaI CCWGG 1 cut(s) 156
NdeII GATC 4 cut(s) 47, 255, 621, 827
NlaIII CATG 2 cut(s) 377, 512
NlaIV GGNNCC 2 cut(s) 499, 745
PfeI GAWTC 9 cut(s) 26, 55, 143, 211, 344, 556, 700, 756, 883
PinAI ACCGGT 1 cut(s) 530
PkrI GCNGC 5 cut(s) 430, 433, 769, 793, 904
PleI GAGTC 2 cut(s) 819, 865
PpsI GAGTC 2 cut(s) 819, 865
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 2 cut(s) 499, 745
PstI CTGCAG 1 cut(s) 433
PstNI CAGNNNCTG 2 cut(s) 122, 605
PvuII CAGCTG 1 cut(s) 136
RsaI GTAC 2 cut(s) 338, 535
RsaNI GTAC 2 cut(s) 337, 534
RseI CAYNNNNRTG 2 cut(s) 17, 272
SaqAI TTAA 5 cut(s) 69, 180, 243, 288, 351
SatI GCNGC 5 cut(s) 429, 432, 768, 792, 903
Sau3AI GATC 4 cut(s) 47, 255, 621, 827
ScaI AGTACT 1 cut(s) 338
SchI GAGTC 2 cut(s) 820, 866
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 1 cut(s) 729
SfaNI GCATC 4 cut(s) 159, 160, 178, 931
SfcI CTRYAG 1 cut(s) 429
SmiMI CAYNNNNRTG 2 cut(s) 17, 272
SmlI CTYRAG 2 cut(s) 251, 380
SmoI CTYRAG 2 cut(s) 251, 380
Sse9I AATT 1 cut(s) 367
StyD4I CCNGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 163, 341, 848
TaiI ACGT 2 cut(s) 786, 894
TaqI TCGA 4 cut(s) 102, 270, 478, 888
TasI AATT 1 cut(s) 367
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 9 cut(s) 26, 55, 143, 211, 344, 556, 700, 756, 883
Tru1I TTAA 5 cut(s) 69, 180, 243, 288, 351
Tru9I TTAA 5 cut(s) 69, 180, 243, 288, 351
TscAI CASTG 4 cut(s) 329, 606, 853, 967
TseI GCWGC 5 cut(s) 428, 431, 767, 791, 902
TspDTI ATGAA 5 cut(s) 116, 344, 474, 630, 957
TspRI CASTG 4 cut(s) 329, 606, 853, 967
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
ZrmI AGTACT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.