Rh2BG199400

Microtubule-associated protein RP EB family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015182
Physical Location & Seq
Reverse (-)
18293549 .. 18295707
2159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG199400.1

Sequence Viewer

Length: 864 bp
ATGGACGCGGCTCATCCGGGGATGGTTCCGATGCACAAGGTCAATTTTGACGCCAAGAGCGAGTATGAGATGATCCAGAACTATAAGGTTCTTCAAGATGTCTTCAACAAACTCAAAATTACCAAGCATATTGAGGTAAGCAAGCTTGTGAAAGGAAGGCCGCTCGACAATTTGGAGTTCATGCAATGGATGAAGCGCTACTGTGATTCTATCAATGGCTGCACCAGCTACAATGCTCTGGAGAGGAGAGAAGCTTGCAAGGGAGGTAAAGAAGTAAGCAAGAGAGCTGCCCAATCACAATCATCTGCTAAGGGTACAGCAACTTCCCAAAGAGCTCATTCCTCTCACAATGCTAGAAGAAATGATGTACCCCATTCTGGGAATCAATCAGTAAAGGCCTCAAGACCTTCTTCTAGTGGAGGCCCTGCCCCACCTGCATATGATGAAAAGATCACGGAGTTGAAGCTATCAGTGGATAGCCTAGAGAAGGAGAGAGATTTCTACTTTGCAAAGCTCAGAGATATTGAGATACTATGCCAGACTCCTGAAATTGAACACCTATCTGTTGTTGCTGCTATACAGAAGATTTTATATGCTACAGATGATGATGCGACAGTGGTGGCACAAGCGCAAGCCATGTTGTCTGGCCCCTCAAAGGAAGCAGAGCCCTCGAGCCCCATTGCTGAGCCATTGAGTCCCATTGCTGAGGCTTCAGATGAGAAAGCAAGTTCGGAGACCCAGAAGAGGAAAACCATAAACAACTTTGATGTCGATGCTGCTGCCATGGCAACATTATCTCCAAGGCAAAGGGTCTCTGATGCTTCTGATGTCCATTGTAGTGGGTCACCTCTGATGACTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005819 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030312 GO:0030496 GO:0030865 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CH EB1

Protein Analysis

287

Amino Acids

31.46

Weight (kDa)

6.72

Isoelectric Point (pI)

61.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EB1 PF03271 161 - 199 1.7e-17 EB1-like C-terminal motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67270
fragaria_vesca FvH4_1g16740
malus_domestica MD02G1178500.v1.1 MD15G1288400.v1.1
prunus_persica Prupe.6G229300_v2.0.a1
pyrus_communis pycom02g14460
rosa_chinensis RchiOBHm_Chr2g0106641
rosa_laevigata RLG00000017525
rosa_multiflora Rmu_sc0006221.1_g000003
rosa_roxburghii Rroxscaffold_2G00136930
rosa_rugosa Rorug02G0137000
rosa_samantha Rh2AG188400 Rh2BG199400 Rh2CG193400 Rh2DG194600
rosa_wichuraiana Rw2G014790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 442
AccBSI CCGCTC 1 cut(s) 163
AccII CGCG 1 cut(s) 8
AciI CCGC 2 cut(s) 8, 161
AclWI GGATC 1 cut(s) 67
AcuI CTGAAG 1 cut(s) 696
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 316, 369
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 5 cut(s) 377, 378, 487, 655, 744
AgsI TTSAA 4 cut(s) 95, 106, 463, 554
AluBI AGCT 7 cut(s) 145, 228, 254, 287, 335, 466, 514
AluI AGCT 7 cut(s) 145, 228, 254, 287, 335, 466, 514
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 2 cut(s) 728, 817
AlwI GGATC 1 cut(s) 67
Ama87I CYCGRG 1 cut(s) 670
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 4 cut(s) 158, 396, 421, 646
ApeKI GCWGC 5 cut(s) 219, 287, 572, 776, 779
AspLEI GCGC 2 cut(s) 198, 631
AspS9I GGNCC 2 cut(s) 422, 647
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 1 cut(s) 837
AvaI CYCGRG 1 cut(s) 670
BanII GRGCYC 3 cut(s) 337, 669, 677
BarI GAAGNNNNNNTAC 2 cut(s) 307, 339
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 337
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 5 cut(s) 206, 274, 559, 763, 766
BccI CCATC 1 cut(s) 16
BcgI CGANNNNNNTGC 4 cut(s) 651, 685, 761, 795
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 2 cut(s) 728, 817
BfaI CTAG 3 cut(s) 354, 414, 482
BfmI CTRYAG 1 cut(s) 597
BfoI RGCGCY 1 cut(s) 199
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 7 cut(s) 9, 161, 220, 288, 573, 777, 780
BlpI GCTNAGC 1 cut(s) 684
BlsI GCNGC 7 cut(s) 10, 162, 221, 289, 574, 778, 781
Bme1390I CCNGG 1 cut(s) 18
BmeT110I CYCGRG 1 cut(s) 670
BmgT120I GGNCC 2 cut(s) 422, 647
BmiI GGNNCC 2 cut(s) 27, 649
BmrFI CCNGG 1 cut(s) 18
BmsI GCATC 4 cut(s) 21, 598, 763, 808
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 1 cut(s) 260
Bpu10I CCTNAGC 2 cut(s) 309, 705
Bpu1102I GCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 385
BpuMI CCSGG 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 2 cut(s) 728, 817
BsaJI CCNNGG 3 cut(s) 17, 783, 800
BsaXI ACNNNNNCTCC 2 cut(s) 781, 811
Bsc4I CCNNNNNNNGG 5 cut(s) 377, 378, 487, 655, 744
Bse3DI GCAATG 3 cut(s) 191, 678, 699
BseDI CCNNGG 3 cut(s) 17, 783, 800
BseGI GGATG 3 cut(s) 13, 27, 195
BseLI CCNNNNNNNGG 5 cut(s) 377, 378, 487, 655, 744
BseMI GCAATG 3 cut(s) 191, 678, 699
BseMII CTCAG 3 cut(s) 529, 675, 696
BseRI GAGGAG 1 cut(s) 259
BseXI GCAGC 5 cut(s) 206, 274, 559, 763, 766
BsgI GTGCAG 1 cut(s) 205
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 4 cut(s) 160, 398, 423, 648
BsiHKAI GWGCWC 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 670
BsiSI CCGG 1 cut(s) 17
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 5 cut(s) 377, 378, 487, 655, 744
BsmAI GTCTC 2 cut(s) 728, 817
BsmFI GGGAC 1 cut(s) 681
BsnI GGCC 4 cut(s) 160, 398, 423, 648
Bso31I GGTCTC 2 cut(s) 728, 817
BsoBI CYCGRG 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 337, 669, 677
Bsp143I GATC 2 cut(s) 72, 450
Bsp1720I GCTNAGC 1 cut(s) 684
Bsp19I CCATGG 1 cut(s) 783
BspACI CCGC 2 cut(s) 8, 161
BspANI GGCC 4 cut(s) 160, 398, 423, 648
BspCNI CTCAG 3 cut(s) 528, 676, 697
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 2 cut(s) 27, 649
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 1 cut(s) 67
BspTNI GGTCTC 2 cut(s) 728, 817
BsrBI CCGCTC 1 cut(s) 163
BsrDI GCAATG 3 cut(s) 191, 678, 699
BssECI CCNNGG 3 cut(s) 17, 783, 800
BssMI GATC 2 cut(s) 72, 450
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 783, 800
Bst4CI ACNGT 2 cut(s) 203, 616
Bst6I CTCTTC 1 cut(s) 737
BstACI GRCGYC 1 cut(s) 51
BstC8I GCNNGC 3 cut(s) 143, 256, 633
BstDEI CTNAG 4 cut(s) 309, 515, 684, 705
BstDSI CCRYGG 1 cut(s) 783
BstEII GGTNACC 1 cut(s) 843
BstENI CCTNNNNNAGG 1 cut(s) 485
BstF5I GGATG 3 cut(s) 13, 27, 195
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 2 cut(s) 198, 631
BstKTI GATC 2 cut(s) 75, 453
BstMAI GTCTC 2 cut(s) 728, 817
BstMBI GATC 2 cut(s) 72, 450
BstMWI GCNNNNNNNGC 3 cut(s) 225, 434, 785
BstPI GGTNACC 1 cut(s) 843
BstSCI CCNGG 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 597
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 5 cut(s) 206, 274, 559, 763, 766
BstV2I GAAGAC 1 cut(s) 94
BstXI CCANNNNNNTGG 1 cut(s) 839
BsuRI GGCC 4 cut(s) 160, 398, 423, 648
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 3 cut(s) 13, 27, 195
BtsIMutI CAGTG 2 cut(s) 477, 621
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 3 cut(s) 143, 256, 633
CfoI GCGC 2 cut(s) 198, 631
Cfr13I GGNCC 2 cut(s) 422, 647
CseI GACGC 2 cut(s) 14, 59
Csp6I GTAC 2 cut(s) 315, 368
CviAII CATG 3 cut(s) 181, 637, 784
CviQI GTAC 2 cut(s) 315, 368
DdeI CTNAG 4 cut(s) 309, 515, 684, 705
DpnI GATC 2 cut(s) 74, 452
DpnII GATC 2 cut(s) 72, 450
Eam1104I CTCTTC 1 cut(s) 737
EarI CTCTTC 1 cut(s) 737
Ecl136II GAGCTC 1 cut(s) 335
Eco130I CCWWGG 2 cut(s) 783, 800
Eco147I AGGCCT 1 cut(s) 398
Eco24I GRGCYC 3 cut(s) 337, 669, 677
Eco31I GGTCTC 2 cut(s) 728, 817
Eco47III AGCGCT 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 670
Eco91I GGTNACC 1 cut(s) 843
EcoICRI GAGCTC 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 485
EcoO109I RGGNCCY 1 cut(s) 422
EcoO65I GGTNACC 1 cut(s) 843
EcoT14I CCWWGG 2 cut(s) 783, 800
EcoT38I GRGCYC 3 cut(s) 337, 669, 677
ErhI CCWWGG 2 cut(s) 783, 800
FaeI CATG 3 cut(s) 184, 640, 787
FalI AAGNNNNNCTT 2 cut(s) 394, 426
FaqI GGGAC 1 cut(s) 681
FatI CATG 3 cut(s) 180, 636, 783
FauNDI CATATG 1 cut(s) 439
Fnu4HI GCNGC 7 cut(s) 9, 161, 220, 288, 573, 777, 780
FokI GGATG 2 cut(s) 34, 202
FriOI GRGCYC 3 cut(s) 337, 669, 677
Fsp4HI GCNGC 7 cut(s) 9, 161, 220, 288, 573, 777, 780
FspBI CTAG 3 cut(s) 354, 414, 482
GlaI GCGC 2 cut(s) 197, 630
GluI GCNGC 7 cut(s) 9, 161, 220, 288, 573, 777, 780
GsuI CTGGAG 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 4 cut(s) 160, 398, 423, 648
HapII CCGG 1 cut(s) 17
HgaI GACGC 2 cut(s) 14, 59
HhaI GCGC 2 cut(s) 198, 631
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 3 cut(s) 184, 640, 787
Hin6I GCGC 2 cut(s) 196, 629
HinP1I GCGC 2 cut(s) 196, 629
HindIII AAGCTT 2 cut(s) 143, 252
HinfI GANTC 4 cut(s) 206, 382, 541, 694
HpaII CCGG 1 cut(s) 17
HphI GGTGA 1 cut(s) 837
Hpy188I TCNGA 7 cut(s) 30, 518, 715, 733, 817, 826, 852
Hpy188III TCNNGA 5 cut(s) 76, 95, 239, 402, 545
HpyAV CCTTC 3 cut(s) 150, 417, 481
HpyCH4III ACNGT 2 cut(s) 203, 616
HpyCH4V TGCA 6 cut(s) 34, 184, 222, 258, 437, 509
HpyF10VI GCNNNNNNNGC 3 cut(s) 225, 434, 785
HpyF3I CTNAG 4 cut(s) 309, 515, 684, 705
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 3 cut(s) 184, 640, 787
HspAI GCGC 2 cut(s) 196, 629
Kzo9I GATC 2 cut(s) 72, 450
Lsp1109I GCAGC 5 cut(s) 206, 274, 559, 763, 766
LweI GCATC 4 cut(s) 21, 598, 763, 808
MaeI CTAG 3 cut(s) 354, 414, 482
MaeIII GTNAC 1 cut(s) 843
MalI GATC 2 cut(s) 74, 452
MbiI CCGCTC 1 cut(s) 163
MboI GATC 2 cut(s) 72, 450
MboII GAAGA 6 cut(s) 83, 94, 369, 402, 595, 754
MhlI GDGCHC 3 cut(s) 337, 669, 677
MluCI AATT 4 cut(s) 43, 117, 169, 549
MlyI GAGTC 2 cut(s) 535, 703
MslI CAYNNNNRTG 1 cut(s) 837
MspI CCGG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 18
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 3 cut(s) 225, 434, 785
NciI CCSGG 1 cut(s) 18
NcoI CCATGG 1 cut(s) 783
NdeI CATATG 1 cut(s) 439
NdeII GATC 2 cut(s) 72, 450
NlaIII CATG 3 cut(s) 184, 640, 787
NlaIV GGNNCC 2 cut(s) 27, 649
NmuCI GTSAC 1 cut(s) 843
PaeR7I CTCGAG 1 cut(s) 670
PaqCI CACCTGC 1 cut(s) 442
PceI AGGCCT 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 2 cut(s) 206, 382
PkrI GCNGC 7 cut(s) 10, 162, 221, 289, 574, 778, 781
PleI GAGTC 2 cut(s) 535, 702
PpsI GAGTC 2 cut(s) 535, 702
Psp124BI GAGCTC 1 cut(s) 337
PspEI GGTNACC 1 cut(s) 843
PspN4I GGNNCC 2 cut(s) 27, 649
PspPI GGNCC 2 cut(s) 422, 647
PspXI VCTCGAGB 1 cut(s) 670
RsaI GTAC 2 cut(s) 316, 369
RsaNI GTAC 2 cut(s) 315, 368
RseI CAYNNNNRTG 1 cut(s) 837
SacI GAGCTC 1 cut(s) 337
SatI GCNGC 7 cut(s) 9, 161, 220, 288, 573, 777, 780
Sau3AI GATC 2 cut(s) 72, 450
Sau96I GGNCC 2 cut(s) 422, 647
SchI GAGTC 2 cut(s) 535, 703
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 3 cut(s) 337, 669, 677
SfaNI GCATC 4 cut(s) 21, 598, 763, 808
SfcI CTRYAG 1 cut(s) 597
Sfr274I CTCGAG 1 cut(s) 670
SlaI CTCGAG 1 cut(s) 670
SmiMI CAYNNNNRTG 1 cut(s) 837
SmlI CTYRAG 2 cut(s) 400, 670
SmoI CTYRAG 2 cut(s) 400, 670
Sse9I AATT 4 cut(s) 43, 117, 169, 549
SseBI AGGCCT 1 cut(s) 398
SsiI CCGC 2 cut(s) 8, 161
SspMI CTAG 3 cut(s) 354, 414, 482
SstI GAGCTC 1 cut(s) 337
StuI AGGCCT 1 cut(s) 398
StyD4I CCNGG 1 cut(s) 16
StyI CCWWGG 2 cut(s) 783, 800
TaaI ACNGT 2 cut(s) 203, 616
TaqI TCGA 3 cut(s) 165, 671, 771
TasI AATT 4 cut(s) 43, 117, 169, 549
TauI GCSGC 2 cut(s) 11, 163
TfiI GAWTC 2 cut(s) 206, 382
TscAI CASTG 2 cut(s) 477, 621
TseFI GTSAC 1 cut(s) 843
TseI GCWGC 5 cut(s) 219, 287, 572, 776, 779
Tsp45I GTSAC 1 cut(s) 843
TspDTI ATGAA 3 cut(s) 169, 206, 459
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 2 cut(s) 477, 621
XagI CCTNNNNNAGG 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 670
XspI CTAG 3 cut(s) 354, 414, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.