MD15G1288400.v1.1

Microtubule-associated protein RP EB family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015182
Physical Location & Seq
Reverse (-)
26571652 .. 26573793
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1288400.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCGACGAATATTGGTATGATGGACGGCGCTTACTTCGTAGGCAGGACCGAGATCTTGGCCTGGATCAACTCAACTCTCCATCTCAATCTCAGCAAAGTCGAGGAGGCGTGTTCCGGTGCCGTTCACTGCCAACTCATGGATGCCGTTCATAACGGTATTGTGCCGATGCACAAGGTCAATTTTGATGCCAAGAGCGAGTACGAGATGATTCAGAACTACAAGGTCCTTCAGGACGTTTTCAACAAACTCAAAATCACTAAGCACATTGAAGTGAGCAAGCTTGTGAAAGGAAGGCCGCTCGATAATCTGGAGTTCATGCAATGGATGAAGCGGTACTGCGATTCTGTCAATGGAGGCAGTATGAACAATTACAATGCTCTGGAGAGGAGAGAAGCTTGCAAAGGAGGGAAAGAAGCTTGCAAAGTTGGGAAAGAAGGAAACAAGAGAGGTGCCCAATTGCAATCATCAGCTAAGGGTTCAGCAGCTCCCCAAAGATCTCAGTCCTCCCACAATGCTCGAAGAAATGATGTAGCACCTTCTGTGAACTCCACAAGTCAATCAGGGAAGACCTCGAGGCCTTCTTCCAGTGGAGGCCCTACAACCCCTGCGTATGATGAACAGGTGATCACGGAGTTGAAGCTGTCGGTGGATAGCCTTGAGAAGGAGAGGGACTTCTACTTTGCGAAGCTCAGAGATATTGAGATACTTTGCCAGAGTCCTGAGATTGAACACCTAACTGTTGTTGCTGCTATACAGAAGATTCTATATGCTACCGATGATAATGCATCGGTGGTGGCAGAAGCTCAAGCCATGCTGTCTGCCCCCCAAAAGGAAGTGGAGCCCTTGAGTCCAATTGCTGAAGCTTCAGATGAGAAGGCGAGTTCAGAGACACAAAAGAGGAAAACTTATCTGAATCTCGACGTCGATGCTGCTGCCATGACGACATTATCTCCGAAACAAAGGATCTCCGACGCTTCTGATGTCCATTGTAGTGGGTCACCCCTTATGACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005819 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0016043 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030312 GO:0030496 GO:0030865 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CH EB1

Protein Analysis

340

Amino Acids

37.2

Weight (kDa)

6.47

Isoelectric Point (pI)

61.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 17 - 108 6.6e-09 Calponin homology (CH) domain
EB1 PF03271 220 - 258 4.7e-17 EB1-like C-terminal motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67270
fragaria_vesca FvH4_1g16740
malus_domestica MD02G1178500.v1.1 MD15G1288400.v1.1
prunus_persica Prupe.6G229300_v2.0.a1
pyrus_communis pycom02g14460
rosa_chinensis RchiOBHm_Chr2g0106641
rosa_laevigata RLG00000017525
rosa_multiflora Rmu_sc0006221.1_g000003
rosa_roxburghii Rroxscaffold_2G00136930
rosa_rugosa Rorug02G0137000
rosa_samantha Rh2AG188400 Rh2BG199400 Rh2CG193400 Rh2DG194600
rosa_wichuraiana Rw2G014790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 927
AbsI CCTCGAGG 1 cut(s) 574
AccB1I GGYRCC 2 cut(s) 119, 452
AccB7I CCANNNNNTGG 1 cut(s) 139
AccBSI CCGCTC 1 cut(s) 301
AciI CCGC 2 cut(s) 299, 334
AclWI GGATC 2 cut(s) 74, 974
AcuI CTGAAG 3 cut(s) 215, 852, 882
AcyI GRCGYC 1 cut(s) 924
AfaI GTAC 2 cut(s) 203, 338
AfiI CCNNNNNNNGG 3 cut(s) 139, 664, 832
AgsI TTSAA 4 cut(s) 244, 272, 640, 731
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 9 cut(s) 283, 398, 419, 473, 488, 643, 691, 806, 866
AluI AGCT 9 cut(s) 283, 398, 419, 473, 488, 643, 691, 806, 866
Alw26I GTCTC 1 cut(s) 884
AlwI GGATC 2 cut(s) 74, 974
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 4 cut(s) 60, 296, 578, 595
ApeKI GCWGC 4 cut(s) 485, 749, 932, 935
ArsI GACNNNNNNTTYG 2 cut(s) 665, 697
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 3 cut(s) 48, 226, 596
AsuHPI GGTGA 2 cut(s) 637, 993
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 2 cut(s) 48, 226
BaeGI GKGCMC 1 cut(s) 457
BanI GGYRCC 2 cut(s) 119, 452
BanII GRGCYC 1 cut(s) 846
BbsI GAAGAC 1 cut(s) 575
BbvI GCAGC 4 cut(s) 497, 736, 919, 922
BccI CCATC 2 cut(s) 16, 90
BceAI ACGGC 3 cut(s) 43, 107, 131
BcgI CGANNNNNNTGC 6 cut(s) 767, 801, 911, 917, 945, 951
BciT130I CCWGG 1 cut(s) 64
BclI TGATCA 1 cut(s) 627
BcoDI GTCTC 1 cut(s) 884
BfoI RGCGCY 1 cut(s) 33
BglII AGATCT 2 cut(s) 54, 497
BisI GCNGC 5 cut(s) 299, 486, 750, 933, 936
BlsI GCNGC 5 cut(s) 300, 487, 751, 934, 937
Bme1390I CCNGG 1 cut(s) 64
Bme18I GGWCC 2 cut(s) 48, 226
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 3 cut(s) 48, 226, 596
BmiI GGNNCC 3 cut(s) 121, 454, 843
BmrFI CCNGG 1 cut(s) 64
BmsI GCATC 5 cut(s) 133, 159, 178, 797, 919
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 2 cut(s) 332, 404
Bpu10I CCTNAGC 1 cut(s) 474
BpuEI CTTGAG 3 cut(s) 680, 792, 868
BsaHI GRCGYC 1 cut(s) 924
BsaWI WCCGGW 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 937, 967
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 664, 832
Bse1I ACTGG 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 329
BseBI CCWGG 1 cut(s) 64
BseGI GGATG 2 cut(s) 148, 333
BseLI CCNNNNNNNGG 3 cut(s) 139, 664, 832
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 4 cut(s) 106, 515, 706, 714
BseNI ACTGG 1 cut(s) 588
BseRI GAGGAG 2 cut(s) 119, 403
BseSI GKGCMC 1 cut(s) 457
BseXI GCAGC 4 cut(s) 497, 736, 919, 922
BshFI GGCC 4 cut(s) 62, 298, 580, 597
BshNI GGYRCC 2 cut(s) 119, 452
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 1 cut(s) 117
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 3 cut(s) 139, 664, 832
BsmAI GTCTC 1 cut(s) 884
BsmFI GGGAC 1 cut(s) 686
BsnI GGCC 4 cut(s) 62, 298, 580, 597
BsoBI CYCGRG 1 cut(s) 574
Bsp1286I GDGCHC 2 cut(s) 457, 846
Bsp143I GATC 5 cut(s) 54, 66, 497, 627, 966
BspACI CCGC 2 cut(s) 299, 334
BspANI GGCC 4 cut(s) 62, 298, 580, 597
BspCNI CTCAG 4 cut(s) 105, 514, 705, 715
BspLI GGNNCC 3 cut(s) 121, 454, 843
BspPI GGATC 2 cut(s) 74, 974
BspT107I GGYRCC 2 cut(s) 119, 452
BsrBI CCGCTC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 329
BsrI ACTGG 1 cut(s) 588
BssMI GATC 5 cut(s) 54, 66, 497, 627, 966
BssNI GRCGYC 1 cut(s) 924
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 158, 742
BstACI GRCGYC 1 cut(s) 924
BstC8I GCNNGC 3 cut(s) 281, 400, 421
BstDEI CTNAG 6 cut(s) 92, 261, 474, 501, 692, 723
BstEII GGTNACC 1 cut(s) 999
BstENI CCTNNNNNAGG 1 cut(s) 662
BstF5I GGATG 2 cut(s) 148, 333
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 5 cut(s) 57, 69, 500, 630, 969
BstMAI GTCTC 1 cut(s) 884
BstMBI GATC 5 cut(s) 54, 66, 497, 627, 966
BstNI CCWGG 1 cut(s) 64
BstPI GGTNACC 1 cut(s) 999
BstSCI CCNGG 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 457
BstV1I GCAGC 4 cut(s) 497, 736, 919, 922
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 3 cut(s) 54, 497, 966
BstXI CCANNNNNNTGG 1 cut(s) 995
BstYI RGATCY 3 cut(s) 54, 497, 966
BsuRI GGCC 4 cut(s) 62, 298, 580, 597
BtsCI GGATG 2 cut(s) 148, 333
BtsI GCAGTG 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 127, 595
Cac8I GCNNGC 3 cut(s) 281, 400, 421
CfoI GCGC 1 cut(s) 32
Cfr13I GGNCC 3 cut(s) 48, 226, 596
CseI GACGC 1 cut(s) 983
Csp6I GTAC 2 cut(s) 202, 337
CviAII CATG 4 cut(s) 139, 319, 814, 940
CviQI GTAC 2 cut(s) 202, 337
DdeI CTNAG 6 cut(s) 92, 261, 474, 501, 692, 723
DpnI GATC 5 cut(s) 56, 68, 499, 629, 968
DpnII GATC 5 cut(s) 54, 66, 497, 627, 966
Eco147I AGGCCT 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 846
Eco47I GGWCC 2 cut(s) 48, 226
Eco57I CTGAAG 3 cut(s) 215, 852, 882
Eco88I CYCGRG 1 cut(s) 574
Eco91I GGTNACC 1 cut(s) 999
EcoNI CCTNNNNNAGG 1 cut(s) 662
EcoO109I RGGNCCY 2 cut(s) 226, 596
EcoO65I GGTNACC 1 cut(s) 999
EcoRII CCWGG 1 cut(s) 62
EcoT22I ATGCAT 1 cut(s) 790
EcoT38I GRGCYC 1 cut(s) 846
FaeI CATG 4 cut(s) 142, 322, 817, 943
FaqI GGGAC 1 cut(s) 686
FatI CATG 4 cut(s) 138, 318, 813, 939
FbaI TGATCA 1 cut(s) 627
Fnu4HI GCNGC 5 cut(s) 299, 486, 750, 933, 936
FokI GGATG 2 cut(s) 155, 340
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 5 cut(s) 299, 486, 750, 933, 936
GlaI GCGC 1 cut(s) 31
GluI GCNGC 5 cut(s) 299, 486, 750, 933, 936
GsuI CTGGAG 2 cut(s) 332, 404
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 4 cut(s) 62, 298, 580, 597
HapII CCGG 1 cut(s) 117
HgaI GACGC 1 cut(s) 983
HhaI GCGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 924
Hin1II CATG 4 cut(s) 142, 322, 817, 943
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HindIII AAGCTT 4 cut(s) 281, 396, 417, 864
HinfI GANTC 6 cut(s) 211, 344, 718, 763, 850, 916
HpaII CCGG 1 cut(s) 117
HphI GGTGA 2 cut(s) 637, 993
Hpy166II GTNNAC 2 cut(s) 127, 547
Hpy188I TCNGA 8 cut(s) 216, 695, 871, 889, 915, 957, 973, 982
Hpy188III TCNNGA 5 cut(s) 233, 311, 383, 722, 920
Hpy8I GTNNAC 2 cut(s) 127, 547
Hpy99I CGWCG 4 cut(s) 10, 926, 929, 977
HpyAV CCTTC 7 cut(s) 239, 288, 431, 549, 591, 658, 871
HpyCH4III ACNGT 2 cut(s) 158, 742
HpyCH4IV ACGT 2 cut(s) 237, 924
HpyCH4V TGCA 6 cut(s) 172, 322, 402, 423, 463, 788
HpyF3I CTNAG 6 cut(s) 92, 261, 474, 501, 692, 723
HpySE526I ACGT 2 cut(s) 237, 924
Hsp92I GRCGYC 1 cut(s) 924
Hsp92II CATG 4 cut(s) 142, 322, 817, 943
HspAI GCGC 1 cut(s) 30
Ksp22I TGATCA 1 cut(s) 627
Kzo9I GATC 5 cut(s) 54, 66, 497, 627, 966
LmnI GCTCC 2 cut(s) 493, 841
Lsp1109I GCAGC 4 cut(s) 497, 736, 919, 922
LweI GCATC 5 cut(s) 133, 159, 178, 797, 919
MaeII ACGT 2 cut(s) 237, 924
MaeIII GTNAC 1 cut(s) 999
MalI GATC 5 cut(s) 56, 68, 499, 629, 968
MbiI CCGCTC 1 cut(s) 301
MboI GATC 5 cut(s) 54, 66, 497, 627, 966
MboII GAAGA 4 cut(s) 534, 576, 580, 772
MfeI CAATTG 2 cut(s) 458, 855
MflI RGATCY 3 cut(s) 54, 497, 966
MhlI GDGCHC 2 cut(s) 457, 846
MluCI AATT 4 cut(s) 181, 370, 458, 855
MlyI GAGTC 2 cut(s) 727, 859
MmeI TCCRAC 1 cut(s) 996
Mph1103I ATGCAT 1 cut(s) 790
MslI CAYNNNNRTG 2 cut(s) 272, 993
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 64
MunI CAATTG 2 cut(s) 458, 855
MvaI CCWGG 1 cut(s) 64
NdeII GATC 5 cut(s) 54, 66, 497, 627, 966
NlaIII CATG 4 cut(s) 142, 322, 817, 943
NlaIV GGNNCC 3 cut(s) 121, 454, 843
NmuCI GTSAC 1 cut(s) 999
NsiI ATGCAT 1 cut(s) 790
PaeR7I CTCGAG 1 cut(s) 574
PceI AGGCCT 1 cut(s) 580
PfeI GAWTC 4 cut(s) 211, 344, 763, 916
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 5 cut(s) 300, 487, 751, 934, 937
PleI GAGTC 2 cut(s) 726, 858
PpsI GAGTC 2 cut(s) 726, 858
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 62
PspEI GGTNACC 1 cut(s) 999
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 3 cut(s) 121, 454, 843
PspPI GGNCC 3 cut(s) 48, 226, 596
PspPPI RGGWCCY 1 cut(s) 226
PspXI VCTCGAGB 1 cut(s) 574
PsuI RGATCY 3 cut(s) 54, 497, 966
RsaI GTAC 2 cut(s) 203, 338
RsaNI GTAC 2 cut(s) 202, 337
RseI CAYNNNNRTG 2 cut(s) 272, 993
SatI GCNGC 5 cut(s) 299, 486, 750, 933, 936
Sau3AI GATC 5 cut(s) 54, 66, 497, 627, 966
Sau96I GGNCC 3 cut(s) 48, 226, 596
SchI GAGTC 2 cut(s) 727, 859
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 2 cut(s) 457, 846
SfaNI GCATC 5 cut(s) 133, 159, 178, 797, 919
Sfr274I CTCGAG 1 cut(s) 574
SinI GGWCC 2 cut(s) 48, 226
SlaI CTCGAG 1 cut(s) 574
SmiMI CAYNNNNRTG 2 cut(s) 272, 993
SmlI CTYRAG 4 cut(s) 574, 659, 807, 847
SmoI CTYRAG 4 cut(s) 574, 659, 807, 847
Sse9I AATT 4 cut(s) 181, 370, 458, 855
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 2 cut(s) 299, 334
SspI AATATT 1 cut(s) 13
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 1 cut(s) 62
TaaI ACNGT 2 cut(s) 158, 742
TaiI ACGT 2 cut(s) 240, 927
TaqI TCGA 6 cut(s) 102, 303, 520, 575, 921, 927
TaqII GACCGA 1 cut(s) 65
TasI AATT 4 cut(s) 181, 370, 458, 855
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 4 cut(s) 211, 344, 763, 916
TscAI CASTG 2 cut(s) 134, 595
TseFI GTSAC 1 cut(s) 999
TseI GCWGC 4 cut(s) 485, 749, 932, 935
Tsp45I GTSAC 1 cut(s) 999
TspDTI ATGAA 5 cut(s) 140, 307, 344, 380, 633
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 2 cut(s) 134, 595
Van91I CCANNNNNTGG 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 48, 226
XagI CCTNNNNNAGG 1 cut(s) 662
XhoI CTCGAG 1 cut(s) 574
ZraI GACGTC 1 cut(s) 925
Zsp2I ATGCAT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.