AT5G67550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012316
Physical Location & Seq
Reverse (-)
26946732 .. 26949184
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67550.1

Sequence Viewer

Length: 1530 bp
ATGACAAAATCAAGAATAACCGGAGCTCTGTCTCAGCTTCTTTTAACGGCTGTGATCTCTCCGATTTTGGCGGTTTCAAGTTTATCAGACGGTGGAGATCATGAGAGGTTTAAGAGACGCGATCCTCTCAACAGCTTTAGATATTACGACGGAGGTTTCAATGTTCGAAACAAACATTATTGGGCAGCTACTGCATTTACGGGAATCCACGGTTACGCCGTCGCCGGAGTGTTGATTATTGTCGGTATATGTTTGGGACTTTACGTAGCATTCTCCGACAAAAGAAGACGTGTTTCTTCCACTCGCCGACGATATCTCGACCGTTATTATCTTCCTCTGTTCTTACTTCTCCTCCTTTTCATGTTTCTCTCTGTGGTAACGACAGGGATAGTGATAGCAGCGAACCAGAGGTCGAAGAACAGAACAGAGGAGATGAAAGAAACGATAGACAAAGCAGGAGAAGATGTTAATCAAAACATTCGTACAGTGATAGTGTCGTTAACGAAGATACAGTACTTGTTGCTTCCATACGATCAGAACACGACTCATCTTCTCAACGTCACCACTCATCGACTCGGCAAAGGTTCTCGCCTTATCCAAAGCTTCCTTCATCATAAGGGTCGTTCCATTGATCTCGCCATCAAAATCTCGTATGTATCTCATCTTATGATTACATCGACCAACTTGTTCCTTCTGCTTCTAGCCTTTTTACCTCTATTGCTTCACTGGCACCCTGGATTCATCATGGTGATATTCTTGTGTTGGATCATAACAACTCTTTGTTGGGTTCTAACTGGATTCGATTTCTTTATACACACCTTCGCAGAAGACCTTTGTTCAGCTTTCAATGGTTTCGTACAAAACCCACGAAATAGCACACTGACTAATCTTTTCCCTTGTATGGATCCTCTTCATTCTGATAAAACCCTAATCGAGATAAGCCTGATGATTCATAACTTTATTACCCAGTTAAACTCAAAAGTAGCAGAGTCAATGCGTTCTAATGCGTTGACTGATCGGAGCAATACTGTCTCGTGGGCGCCTGAATCTGGAATCATATGCGATCCGTTTGTCGGACAACAGATTAACAGCTACACACCACAAAGCTGCTCCAATGGCGCCATTCCAATCGGCGAATTCCCAAATATCCTTTCGAGATTCACATGCCACGACAAAGATCCACCGGAAACTTGCAGAATCACCGGAAAATTCATCCCGGAAGCAGCTTACCTAAAGGTCTATGCTTACAGCAACTCAGCACAAGGCATGCTAGACATTTTGCCTTCTTTTCAAAACCTAACAGAATGCCTTGCCGTGAAAGACACGTTATCGAGCATCGTCTCTAACCAATGTGATCCCTTTAGGGCTTCGATGTATCGCCTTTGGGCATCGATCCTTGCTCTGTCTCTTATCATGGTGGTTCTAGTTTTGCTCTTCCTCGCTAAAGCTTTCCAGGAGAAAGGAAAGAGTTTTGCTTGGTTTTCTATTCATCCAACTTCTTCTGCAGAAATACGACAAGTGAATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

509

Amino Acids

57.34

Weight (kDa)

9.09

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67550
fragaria_vesca FvH4_1g17730 FvH4_1g17750 FvH4_1g17750
malus_domestica MD02G1186300.v1.1
prunus_persica Prupe.6G220300_v2.0.a1 Prupe.6G220300_v2.0.a1
pyrus_communis pycom02g15130 pycom02g15140
rosa_chinensis RchiOBHm_Chr2g0108061
rosa_laevigata RLG00000017637
rosa_roxburghii Rroxscaffold_2G00135650
rosa_rugosa Rorug02G0149900 Rorug02G0150000
rosa_samantha Rh2AG199200 Rh2BG212000 Rh2CG203800 Rh2DG206800
rosa_wichuraiana Rw2G015810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 729, 1039, 1118
AccII CGCG 1 cut(s) 120
AciI CCGC 1 cut(s) 71
AclWI GGATC 8 cut(s) 116, 773, 899, 912, 1058, 1172, 1349, 1387
AcsI RAATTY 2 cut(s) 1136, 1208
AcyI GRCGYC 2 cut(s) 1040, 1119
AfaI GTAC 3 cut(s) 484, 515, 858
AfiI CCNNNNNNNGG 3 cut(s) 901, 1049, 1073
AflIII ACRYGT 2 cut(s) 289, 1323
AgsI TTSAA 4 cut(s) 78, 160, 847, 1292
AjiI CACGTC 1 cut(s) 290
AjnI CCWGG 2 cut(s) 733, 1452
AjuI GAANNNNNNNTTGG 4 cut(s) 591, 623, 1135, 1167
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 5 cut(s) 36, 109, 1036, 1345, 1410
AlwI GGATC 8 cut(s) 116, 773, 899, 912, 1058, 1172, 1349, 1387
AlwNI CAGNNNCTG 1 cut(s) 191
ApeKI GCWGC 4 cut(s) 185, 398, 1107, 1223
ApoI RAATTY 2 cut(s) 1136, 1208
ArsI GACNNNNNNTTYG 2 cut(s) 395, 427
AspLEI GCGC 2 cut(s) 1042, 1121
AsuC2I CCSGG 1 cut(s) 1217
AsuHPI GGTGA 3 cut(s) 553, 760, 1192
AsuII TTCGAA 1 cut(s) 166
BamHI GGATCC 1 cut(s) 904
BanI GGYRCC 3 cut(s) 729, 1039, 1118
BanII GRGCYC 1 cut(s) 28
BarI GAAGNNNNNNTAC 2 cut(s) 497, 529
BauI CACGAG 1 cut(s) 1033
BbsI GAAGAC 2 cut(s) 292, 834
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 4 cut(s) 197, 410, 1094, 1235
BccI CCATC 1 cut(s) 647
BceAI ACGGC 3 cut(s) 63, 203, 1298
BciT130I CCWGG 2 cut(s) 735, 1454
BcnI CCSGG 1 cut(s) 1217
BcoDI GTCTC 5 cut(s) 36, 109, 1036, 1345, 1410
BfaI CTAG 3 cut(s) 701, 1271, 1424
BfmI CTRYAG 1 cut(s) 1503
BfoI RGCGCY 2 cut(s) 1043, 1122
BisI GCNGC 4 cut(s) 186, 399, 1108, 1224
BlsI GCNGC 4 cut(s) 187, 400, 1109, 1225
BmcAI AGTACT 1 cut(s) 515
Bme1390I CCNGG 3 cut(s) 735, 1217, 1454
BmgBI CACGTC 1 cut(s) 290
BmiI GGNNCC 4 cut(s) 731, 906, 1041, 1120
BmrFI CCNGG 3 cut(s) 735, 1217, 1454
BmrI ACTGGG 1 cut(s) 961
BmsI GCATC 2 cut(s) 1344, 1397
BmuI ACTGGG 1 cut(s) 961
BpiI GAAGAC 2 cut(s) 292, 834
Bpu14I TTCGAA 1 cut(s) 166
BpuMI CCSGG 1 cut(s) 1217
Bsa29I ATCGAT 1 cut(s) 1391
BsaAI YACGTR 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 468
BsaHI GRCGYC 2 cut(s) 1040, 1119
BsaJI CCNNGG 2 cut(s) 208, 733
BsaWI WCCGGW 3 cut(s) 20, 1183, 1202
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 450, 480
Bsc4I CCNNNNNNNGG 3 cut(s) 901, 1049, 1073
Bse1I ACTGG 3 cut(s) 731, 799, 967
Bse8I GATNNNNATC 1 cut(s) 468
BseBI CCWGG 2 cut(s) 735, 1454
BseCI ATCGAT 1 cut(s) 1391
BseDI CCNNGG 2 cut(s) 208, 733
BseGI GGATG 2 cut(s) 1212, 1489
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 3 cut(s) 901, 1049, 1073
BseMII CTCAG 2 cut(s) 47, 1269
BseNI ACTGG 3 cut(s) 731, 799, 967
BseRI GAGGAG 2 cut(s) 341, 443
BseXI GCAGC 4 cut(s) 197, 410, 1094, 1235
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 322
BshNI GGYRCC 3 cut(s) 729, 1039, 1118
BshVI ATCGAT 1 cut(s) 1391
BsiEI CGRYCG 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 5 cut(s) 21, 225, 1184, 1203, 1217
BslFI GGGAC 1 cut(s) 270
BslI CCNNNNNNNGG 3 cut(s) 901, 1049, 1073
BsmAI GTCTC 5 cut(s) 36, 109, 1036, 1345, 1410
BsmBI CGTCTC 2 cut(s) 109, 1345
BsmFI GGGAC 1 cut(s) 270
BsmI GAATGC 2 cut(s) 269, 1310
Bsp119I TTCGAA 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 28
BspACI CCGC 1 cut(s) 71
BspCNI CTCAG 2 cut(s) 46, 1268
BspDI ATCGAT 1 cut(s) 1391
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 731, 906, 1041, 1120
BspMAI CTGCAG 1 cut(s) 1507
BspPI GGATC 8 cut(s) 116, 773, 899, 912, 1058, 1172, 1349, 1387
BspQI GCTCTTC 1 cut(s) 1439
BspT104I TTCGAA 1 cut(s) 166
BspT107I GGYRCC 3 cut(s) 729, 1039, 1118
BsrI ACTGG 3 cut(s) 731, 799, 967
BssECI CCNNGG 2 cut(s) 208, 733
BssNI GRCGYC 2 cut(s) 1040, 1119
BssSI CACGAG 1 cut(s) 1033
Bst2BI CACGAG 1 cut(s) 1033
Bst2UI CCWGG 2 cut(s) 735, 1454
Bst4CI ACNGT 6 cut(s) 92, 212, 323, 487, 513, 1030
Bst6I CTCTTC 2 cut(s) 915, 1439
BstACI GRCGYC 2 cut(s) 1040, 1119
BstAPI GCANNNNNTGC 1 cut(s) 191
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 1268
BstDEI CTNAG 2 cut(s) 33, 1255
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 2 cut(s) 1212, 1489
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 2 cut(s) 1043, 1122
BstHHI GCGC 2 cut(s) 1042, 1121
BstMAI GTCTC 5 cut(s) 36, 109, 1036, 1345, 1410
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 3 cut(s) 191, 727, 1116
BstNI CCWGG 2 cut(s) 735, 1454
BstNSI RCATGY 2 cut(s) 1167, 1270
BstSCI CCNGG 3 cut(s) 733, 1215, 1452
BstSFI CTRYAG 1 cut(s) 1503
BstSNI TACGTA 1 cut(s) 265
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 197, 410, 1094, 1235
BstV2I GAAGAC 2 cut(s) 292, 834
BstX2I RGATCY 2 cut(s) 904, 1177
BstYI RGATCY 2 cut(s) 904, 1177
Bsu15I ATCGAT 1 cut(s) 1391
BsuTUI ATCGAT 1 cut(s) 1391
BtgI CCRYGG 1 cut(s) 208
BtrI CACGTC 1 cut(s) 290
BtsCI GGATG 2 cut(s) 1212, 1489
BtsIMutI CAGTG 3 cut(s) 492, 724, 878
Cac8I GCNNGC 1 cut(s) 1268
CaiI CAGNNNCTG 1 cut(s) 191
CciI TCATGA 1 cut(s) 100
CfoI GCGC 2 cut(s) 1042, 1121
ClaI ATCGAT 1 cut(s) 1391
CseI GACGC 1 cut(s) 126
Csp6I GTAC 3 cut(s) 483, 514, 857
CviAII CATG 6 cut(s) 101, 361, 745, 1164, 1267, 1414
CviQI GTAC 3 cut(s) 483, 514, 857
DdeI CTNAG 2 cut(s) 33, 1255
DinI GGCGCC 2 cut(s) 1041, 1120
Eam1104I CTCTTC 2 cut(s) 915, 1439
EarI CTCTTC 2 cut(s) 915, 1439
Ecl136II GAGCTC 1 cut(s) 26
Eco105I TACGTA 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 28
Eco32I GATATC 1 cut(s) 314
Eco53kI GAGCTC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 1136
EcoRII CCWGG 2 cut(s) 733, 1452
EcoRV GATATC 1 cut(s) 314
EcoT38I GRGCYC 1 cut(s) 28
EgeI GGCGCC 2 cut(s) 1041, 1120
EheI GGCGCC 2 cut(s) 1041, 1120
Esp3I CGTCTC 2 cut(s) 109, 1345
FaeI CATG 6 cut(s) 104, 364, 748, 1167, 1270, 1417
FaqI GGGAC 1 cut(s) 270
FatI CATG 6 cut(s) 100, 360, 744, 1163, 1266, 1413
FauNDI CATATG 1 cut(s) 1058
Fnu4HI GCNGC 4 cut(s) 186, 399, 1108, 1224
FokI GGATG 2 cut(s) 1199, 1476
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 4 cut(s) 186, 399, 1108, 1224
FspBI CTAG 3 cut(s) 701, 1271, 1424
GlaI GCGC 2 cut(s) 1041, 1120
GluI GCNGC 4 cut(s) 186, 399, 1108, 1224
HaeII RGCGCY 2 cut(s) 1043, 1122
HapII CCGG 5 cut(s) 21, 225, 1184, 1203, 1217
HgaI GACGC 1 cut(s) 126
HhaI GCGC 2 cut(s) 1042, 1121
Hin1I GRCGYC 2 cut(s) 1040, 1119
Hin1II CATG 6 cut(s) 104, 364, 748, 1167, 1270, 1417
Hin6I GCGC 2 cut(s) 1040, 1119
HinP1I GCGC 2 cut(s) 1040, 1119
HincII GTYRAC 2 cut(s) 501, 1011
HindII GTYRAC 2 cut(s) 501, 1011
HindIII AAGCTT 2 cut(s) 601, 1446
HpaI GTTAAC 1 cut(s) 501
HpaII CCGG 5 cut(s) 21, 225, 1184, 1203, 1217
HphI GGTGA 3 cut(s) 553, 760, 1192
Hpy166II GTNNAC 2 cut(s) 501, 1011
Hpy188I TCNGA 7 cut(s) 63, 88, 277, 537, 919, 1020, 1076
Hpy188III TCNNGA 6 cut(s) 12, 101, 317, 934, 1050, 1155
Hpy8I GTNNAC 2 cut(s) 501, 1011
Hpy99I CGWCG 3 cut(s) 152, 224, 312
HpyAV CCTTC 4 cut(s) 617, 701, 829, 1293
HpyCH4III ACNGT 6 cut(s) 92, 212, 323, 487, 513, 1030
HpyCH4IV ACGT 4 cut(s) 264, 289, 558, 1325
HpyCH4V TGCA 3 cut(s) 194, 1194, 1505
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 727, 1116
HpyF3I CTNAG 2 cut(s) 33, 1255
HpySE526I ACGT 4 cut(s) 264, 289, 558, 1325
Hsp92I GRCGYC 2 cut(s) 1040, 1119
Hsp92II CATG 6 cut(s) 104, 364, 748, 1167, 1270, 1417
HspAI GCGC 2 cut(s) 1040, 1119
KasI GGCGCC 2 cut(s) 1039, 1118
KspAI GTTAAC 1 cut(s) 501
LguI GCTCTTC 1 cut(s) 1439
LmnI GCTCC 3 cut(s) 23, 1020, 1115
Lsp1109I GCAGC 4 cut(s) 197, 410, 1094, 1235
LweI GCATC 2 cut(s) 1344, 1397
MaeI CTAG 3 cut(s) 701, 1271, 1424
MaeII ACGT 4 cut(s) 264, 289, 558, 1325
MaeIII GTNAC 3 cut(s) 212, 376, 559
MflI RGATCY 2 cut(s) 904, 1177
MhlI GDGCHC 1 cut(s) 28
MluCI AATT 2 cut(s) 1136, 1208
Mly113I GGCGCC 2 cut(s) 1040, 1119
MlyI GAGTC 3 cut(s) 538, 567, 998
MmeI TCCRAC 4 cut(s) 300, 743, 1054, 1517
MseI TTAA 6 cut(s) 44, 111, 468, 500, 971, 1086
MslI CAYNNNNRTG 1 cut(s) 746
MspI CCGG 5 cut(s) 21, 225, 1184, 1203, 1217
MspR9I CCNGG 3 cut(s) 735, 1217, 1454
Mva1269I GAATGC 2 cut(s) 269, 1310
MvaI CCWGG 2 cut(s) 735, 1454
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 191, 727, 1116
NarI GGCGCC 2 cut(s) 1040, 1119
NciI CCSGG 1 cut(s) 1217
NdeI CATATG 1 cut(s) 1058
NlaIII CATG 6 cut(s) 104, 364, 748, 1167, 1270, 1417
NlaIV GGNNCC 4 cut(s) 731, 906, 1041, 1120
NmeAIII GCCGAG 1 cut(s) 555
NmuCI GTSAC 1 cut(s) 559
NspI RCATGY 2 cut(s) 1167, 1270
NspV TTCGAA 1 cut(s) 166
PaeI GCATGC 1 cut(s) 1270
PagI TCATGA 1 cut(s) 100
PciSI GCTCTTC 1 cut(s) 1439
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 2 cut(s) 269, 1310
PfeI GAWTC 8 cut(s) 204, 738, 798, 949, 1046, 1053, 1158, 1197
PfoI TCCNGGA 2 cut(s) 1215, 1452
PkrI GCNGC 4 cut(s) 187, 400, 1109, 1225
PleI GAGTC 3 cut(s) 538, 567, 997
PluTI GGCGCC 2 cut(s) 1043, 1122
PpsI GAGTC 3 cut(s) 538, 567, 997
Ppu21I YACGTR 1 cut(s) 265
Psp124BI GAGCTC 1 cut(s) 28
Psp6I CCWGG 2 cut(s) 733, 1452
PspGI CCWGG 2 cut(s) 733, 1452
PspN4I GGNNCC 4 cut(s) 731, 906, 1041, 1120
PstI CTGCAG 1 cut(s) 1507
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 2 cut(s) 904, 1177
RsaI GTAC 3 cut(s) 484, 515, 858
RsaNI GTAC 3 cut(s) 483, 514, 857
RseI CAYNNNNRTG 1 cut(s) 746
SacI GAGCTC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 1439
SaqAI TTAA 6 cut(s) 44, 111, 468, 500, 971, 1086
SatI GCNGC 4 cut(s) 186, 399, 1108, 1224
ScaI AGTACT 1 cut(s) 515
SchI GAGTC 3 cut(s) 538, 567, 998
ScrFI CCNGG 3 cut(s) 735, 1217, 1454
SduI GDGCHC 1 cut(s) 28
SfaNI GCATC 2 cut(s) 1344, 1397
SfcI CTRYAG 1 cut(s) 1503
SfoI GGCGCC 2 cut(s) 1041, 1120
SfuI TTCGAA 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 746
SnaBI TACGTA 1 cut(s) 265
SphI GCATGC 1 cut(s) 1270
Sse9I AATT 2 cut(s) 1136, 1208
SsiI CCGC 1 cut(s) 71
SspDI GGCGCC 2 cut(s) 1039, 1118
SspMI CTAG 3 cut(s) 701, 1271, 1424
SstI GAGCTC 1 cut(s) 28
StyD4I CCNGG 3 cut(s) 733, 1215, 1452
TaaI ACNGT 6 cut(s) 92, 212, 323, 487, 513, 1030
TaiI ACGT 4 cut(s) 267, 292, 561, 1328
TasI AATT 2 cut(s) 1136, 1208
TatI WGTACW 1 cut(s) 513
TfiI GAWTC 8 cut(s) 204, 738, 798, 949, 1046, 1053, 1158, 1197
Tru1I TTAA 6 cut(s) 44, 111, 468, 500, 971, 1086
Tru9I TTAA 6 cut(s) 44, 111, 468, 500, 971, 1086
TscAI CASTG 3 cut(s) 492, 731, 885
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 4 cut(s) 185, 398, 1107, 1223
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 8 cut(s) 349, 449, 599, 730, 902, 941, 1201, 1478
TspGWI ACGGA 2 cut(s) 165, 1056
TspRI CASTG 3 cut(s) 492, 731, 885
XapI RAATTY 2 cut(s) 1136, 1208
XceI RCATGY 2 cut(s) 1167, 1270
XspI CTAG 3 cut(s) 701, 1271, 1424
ZrmI AGTACT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.