MD02G1186300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012316
Physical Location & Seq
Reverse (-)
16937086 .. 16941126
4041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1186300.v1.1.491

Sequence Viewer

Length: 1500 bp
ATGGCTATCTCGAACAGGGTTCTTGTACCCTTCGTCGTCTTCTTGGTTTCAACGTCAGTGTTTTCACCGGCAAGGGTGGTGTTTGGGGAGCAGAGTGGCTTGAACAGACCCGATCCCTTGCGCCACTTAAAATCCTACAATGGAGGCTACGACATCACAAATAAACATTACTGGGCTTCTACGGCATTTACAGGAGTCCATGGGTACGTGATTGCAGGAGTTTGGTTGCTGTGTGGCCTGGCATTTACGATTTGCATAGCCTTCTTGAAGAAGCCGAGCATTAGGAGCACTTGCCCCATTAAACACTTCTTGGACCGTAACTTCATCTTAATGTTCATACTCCTCCTCCTCTTTACAATTCTAGCCATAGTGGCATCAAGCTTGGTGCTGGTAGAAAATGAAAGATCAATGAAGAGAACAGAAAAAGTGAAGGGAGCCCTAATGGGGGTGGCTCAAAATGCTCGACAAACCATCCGAAAAGTAACAACCGCAATGGGAAATATGCAATACCTTTTGCTGCCTTATGATCCAGCCTTGGCAAGCAGTTTGAATGTCACATCCCGTCAGCTTGGAAAAGACTCGCAAGCTATTCAACGTTTTGTTGATAAAAATGGCCATACAATTGAGCGGATTATTCAAACCCCGTATGTTGCTCATATTGTGGTTGTAGCAGTCAACTTGGTCTTATTGGTCGCTGCAACTGTTCTGCTGTTGCTGCATTGGTACCCAGGACTTGTAATCGTAATCTTTCTGTGCTGGATATTAACAACTCTAATTTGGGTCTTAACTGGCTTTGATTTCTTTCTTCAAAATTTTGCAAAAGATACATGTTCAGCTTTTGAGGGTTTTGAACACAACAGCCACAACAGTAGTCTAAGCTCCATTCTTCCATGCTTAGATGCTAAACGCTCAGAGCAGTTGATGGGTCAAATTGGCTACACCATACACACTTTCATAAATAAGTTGAATTCTAAAGTGACAGAAATTGCTAAGACACTTGGAGTAAACGAAGAAAATGATGATTCAGTTGGATTTCTACGTATATGCAATCCTTTCTCCGGACCACCTAACTACACCTACGTACCACAAAGTTGCTCAAACGACAGTGGCATTCCAGTTGGAAAGTTACCAGAAGCCATTTCAGGAATCACTTGTTACCAAAACGACATGGAAAATTGCAAAAGAAGTGGAAAACTTATTTCCCAGGACAACTACAACATGGCCTGGGCTTTCAGCCACTCTATTCAACATTTTCTAGATATATATCCAACTCTGCAGAGCTTATCAGATTGCACCTTCGTTAAGGATGGAATTTCCGACGTCGTTTCGCATCAATGCAAGCCATTTAAGAATTCACTGAAAATGCTATGGGTCTCTATGCTATCCCTAGCCATTTCCATGGTGTTTTTAGTACTAATTTTGGTCATCAAAGTTTTTCAAGATAGGGGAAGATGTTTCACTAAATTTGCCATCATCCCTTGCTTGCCAGTGACTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

500

Amino Acids

55.64

Weight (kDa)

8.96

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67550
fragaria_vesca FvH4_1g17730 FvH4_1g17750 FvH4_1g17750
malus_domestica MD02G1186300.v1.1
prunus_persica Prupe.6G220300_v2.0.a1 Prupe.6G220300_v2.0.a1
pyrus_communis pycom02g15130 pycom02g15140
rosa_chinensis RchiOBHm_Chr2g0108061
rosa_laevigata RLG00000017637
rosa_roxburghii Rroxscaffold_2G00135650
rosa_rugosa Rorug02G0149900 Rorug02G0150000
rosa_samantha Rh2AG199200 Rh2BG212000 Rh2CG203800 Rh2DG206800
rosa_wichuraiana Rw2G015810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1323
Acc65I GGTACC 1 cut(s) 723
AccB1I GGYRCC 1 cut(s) 723
AccBSI CCGCTC 1 cut(s) 628
AccIII TCCGGA 1 cut(s) 1058
AciI CCGC 2 cut(s) 489, 628
AclI AACGTT 1 cut(s) 595
AclWI GGATC 2 cut(s) 107, 521
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 5 cut(s) 811, 967, 1311, 1351, 1463
AcyI GRCGYC 1 cut(s) 1320
AfaI GTAC 5 cut(s) 27, 206, 725, 1083, 1413
AfiI CCNNNNNNNGG 3 cut(s) 444, 445, 1058
AflIII ACRYGT 1 cut(s) 827
AjnI CCWGG 4 cut(s) 237, 727, 1203, 1223
AluBI AGCT 6 cut(s) 381, 568, 587, 836, 879, 1281
AluI AGCT 6 cut(s) 381, 568, 587, 836, 879, 1281
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 1378
AlwI GGATC 2 cut(s) 107, 521
Aor13HI TCCGGA 1 cut(s) 1058
AoxI GGCC 3 cut(s) 235, 613, 1221
ApeKI GCWGC 3 cut(s) 517, 695, 715
ApoI RAATTY 5 cut(s) 811, 967, 1311, 1351, 1463
Asp718I GGTACC 1 cut(s) 723
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 313, 1061
AsuHPI GGTGA 1 cut(s) 57
AvaII GGWCC 2 cut(s) 313, 1061
BaeI ACNNNNGTAYC 2 cut(s) 1065, 1098
BalI TGGCCA 1 cut(s) 615
BanI GGYRCC 1 cut(s) 723
BanII GRGCYC 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 504, 682, 702
BccI CCATC 4 cut(s) 479, 916, 1301, 1478
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 4 cut(s) 239, 729, 1205, 1225
BcoDI GTCTC 1 cut(s) 1378
BfaI CTAG 3 cut(s) 362, 1256, 1388
BfmI CTRYAG 1 cut(s) 1274
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 3 cut(s) 518, 696, 716
BlsI GCNGC 3 cut(s) 519, 697, 717
BmcAI AGTACT 1 cut(s) 1413
Bme1390I CCNGG 4 cut(s) 239, 729, 1205, 1225
Bme18I GGWCC 2 cut(s) 313, 1061
BmgT120I GGNCC 2 cut(s) 313, 1061
BmiI GGNNCC 2 cut(s) 436, 725
BmrFI CCNGG 4 cut(s) 239, 729, 1205, 1225
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 3 cut(s) 383, 889, 1339
BmuI ACTGGG 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 31
BsaAI YACGTR 3 cut(s) 208, 1040, 1081
BsaBI GATNNNNATC 1 cut(s) 1263
BsaHI GRCGYC 1 cut(s) 1320
BsaI GGTCTC 1 cut(s) 1378
BsaJI CCNNGG 6 cut(s) 199, 534, 727, 1203, 1224, 1398
BsaWI WCCGGW 1 cut(s) 1058
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 3 cut(s) 444, 445, 1058
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 4 cut(s) 176, 793, 1115, 1487
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 1263
BseAI TCCGGA 1 cut(s) 1058
BseBI CCWGG 4 cut(s) 239, 729, 1205, 1225
BseDI CCNNGG 6 cut(s) 199, 534, 727, 1203, 1224, 1398
BseGI GGATG 4 cut(s) 471, 557, 1312, 1473
BseJI GATNNNNATC 1 cut(s) 1263
BseLI CCNNNNNNNGG 3 cut(s) 444, 445, 1058
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 924
BseNI ACTGG 4 cut(s) 176, 793, 1115, 1487
BseRI GAGGAG 3 cut(s) 332, 335, 338
BseXI GCAGC 3 cut(s) 504, 682, 702
BshFI GGCC 3 cut(s) 237, 615, 1223
BshNI GGYRCC 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 2 cut(s) 68, 1059
BslI CCNNNNNNNGG 3 cut(s) 444, 445, 1058
BsmAI GTCTC 1 cut(s) 1378
BsmI GAATGC 1 cut(s) 1110
BsnI GGCC 3 cut(s) 237, 615, 1223
Bso31I GGTCTC 1 cut(s) 1378
Bsp1286I GDGCHC 2 cut(s) 290, 439
Bsp13I TCCGGA 1 cut(s) 1058
Bsp143I GATC 3 cut(s) 112, 404, 526
Bsp19I CCATGG 2 cut(s) 199, 1398
BspACI CCGC 2 cut(s) 489, 628
BspANI GGCC 3 cut(s) 237, 615, 1223
BspCNI CTCAG 1 cut(s) 923
BspEI TCCGGA 1 cut(s) 1058
BspLI GGNNCC 2 cut(s) 436, 725
BspMAI CTGCAG 1 cut(s) 1278
BspPI GGATC 2 cut(s) 107, 521
BspT107I GGYRCC 1 cut(s) 723
BspTNI GGTCTC 1 cut(s) 1378
BsrBI CCGCTC 1 cut(s) 628
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 4 cut(s) 176, 793, 1115, 1487
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 6 cut(s) 199, 534, 727, 1203, 1224, 1398
BssMI GATC 3 cut(s) 112, 404, 526
BssNI GRCGYC 1 cut(s) 1320
BssT1I CCWWGG 3 cut(s) 199, 534, 1398
Bst2UI CCWGG 4 cut(s) 239, 729, 1205, 1225
Bst4CI ACNGT 4 cut(s) 317, 703, 869, 1106
Bst6I CTCTTC 1 cut(s) 407
BstACI GRCGYC 1 cut(s) 1320
BstBAI YACGTR 3 cut(s) 208, 1040, 1081
BstC8I GCNNGC 4 cut(s) 541, 585, 1340, 1484
BstDEI CTNAG 4 cut(s) 875, 895, 910, 990
BstDSI CCRYGG 2 cut(s) 199, 1398
BstF5I GGATG 4 cut(s) 471, 557, 1312, 1473
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 3 cut(s) 115, 407, 529
BstMAI GTCTC 1 cut(s) 1378
BstMBI GATC 3 cut(s) 112, 404, 526
BstMWI GCNNNNNNNGC 5 cut(s) 182, 285, 371, 458, 715
BstNI CCWGG 4 cut(s) 239, 729, 1205, 1225
BstNSI RCATGY 1 cut(s) 831
BstSCI CCNGG 4 cut(s) 237, 727, 1203, 1223
BstSFI CTRYAG 1 cut(s) 1274
BstSNI TACGTA 2 cut(s) 1040, 1081
BstV1I GCAGC 3 cut(s) 504, 682, 702
BstV2I GAAGAC 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 1399
BsuRI GGCC 3 cut(s) 237, 615, 1223
BtgI CCRYGG 2 cut(s) 199, 1398
BtsCI GGATG 4 cut(s) 471, 557, 1312, 1473
BtsIMutI CAGTG 4 cut(s) 63, 1111, 1355, 1494
Cac8I GCNNGC 4 cut(s) 541, 585, 1340, 1484
CfoI GCGC 1 cut(s) 123
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 313, 1061
Csp6I GTAC 5 cut(s) 26, 205, 724, 1082, 1412
CspCI CAANNNNNGTGG 2 cut(s) 1168, 1203
CviAII CATG 6 cut(s) 200, 828, 891, 1168, 1219, 1399
CviQI GTAC 5 cut(s) 26, 205, 724, 1082, 1412
DdeI CTNAG 4 cut(s) 875, 895, 910, 990
DpnI GATC 3 cut(s) 114, 406, 528
DpnII GATC 3 cut(s) 112, 404, 526
EaeI YGGCCR 1 cut(s) 613
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
Eco105I TACGTA 2 cut(s) 1040, 1081
Eco130I CCWWGG 3 cut(s) 199, 534, 1398
Eco24I GRGCYC 1 cut(s) 439
Eco31I GGTCTC 1 cut(s) 1378
Eco47I GGWCC 2 cut(s) 313, 1061
EcoRI GAATTC 2 cut(s) 967, 1351
EcoRII CCWGG 4 cut(s) 237, 727, 1203, 1223
EcoT14I CCWWGG 3 cut(s) 199, 534, 1398
EcoT38I GRGCYC 1 cut(s) 439
ErhI CCWWGG 3 cut(s) 199, 534, 1398
FaeI CATG 6 cut(s) 203, 831, 894, 1171, 1222, 1402
FatI CATG 6 cut(s) 199, 827, 890, 1167, 1218, 1398
Fnu4HI GCNGC 3 cut(s) 518, 696, 716
FokI GGATG 4 cut(s) 458, 544, 1319, 1460
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 3 cut(s) 518, 696, 716
FspBI CTAG 3 cut(s) 362, 1256, 1388
GlaI GCGC 1 cut(s) 122
GluI GCNGC 3 cut(s) 518, 696, 716
HaeIII GGCC 3 cut(s) 237, 615, 1223
HapII CCGG 2 cut(s) 68, 1059
HhaI GCGC 1 cut(s) 123
Hin1I GRCGYC 1 cut(s) 1320
Hin1II CATG 6 cut(s) 203, 831, 894, 1171, 1222, 1402
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 5 cut(s) 195, 578, 1022, 1146, 1492
HpaII CCGG 2 cut(s) 68, 1059
HphI GGTGA 1 cut(s) 57
Hpy166II GTNNAC 2 cut(s) 676, 1006
Hpy188I TCNGA 4 cut(s) 476, 913, 1288, 1318
Hpy188III TCNNGA 6 cut(s) 10, 265, 1059, 1143, 1256, 1439
Hpy8I GTNNAC 2 cut(s) 676, 1006
Hpy99I CGWCG 3 cut(s) 38, 1322, 1325
HpyAV CCTTC 4 cut(s) 40, 271, 424, 1306
HpyCH4III ACNGT 4 cut(s) 317, 703, 869, 1106
HpyCH4IV ACGT 6 cut(s) 53, 207, 595, 1039, 1080, 1320
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 285, 371, 458, 715
HpyF3I CTNAG 4 cut(s) 875, 895, 910, 990
HpySE526I ACGT 6 cut(s) 53, 207, 595, 1039, 1080, 1320
Hsp92I GRCGYC 1 cut(s) 1320
Hsp92II CATG 6 cut(s) 203, 831, 894, 1171, 1222, 1402
HspAI GCGC 1 cut(s) 121
Kpn2I TCCGGA 1 cut(s) 1058
KpnI GGTACC 1 cut(s) 727
Kzo9I GATC 3 cut(s) 112, 404, 526
LmnI GCTCC 4 cut(s) 88, 285, 434, 884
Lsp1109I GCAGC 3 cut(s) 504, 682, 702
LweI GCATC 3 cut(s) 383, 889, 1339
MaeI CTAG 3 cut(s) 362, 1256, 1388
MaeII ACGT 6 cut(s) 53, 207, 595, 1039, 1080, 1320
MaeIII GTNAC 7 cut(s) 317, 481, 553, 976, 1125, 1154, 1489
MalI GATC 3 cut(s) 114, 406, 528
MbiI CCGCTC 1 cut(s) 628
MboI GATC 3 cut(s) 112, 404, 526
MboII GAAGA 7 cut(s) 31, 280, 424, 797, 878, 1022, 1461
MfeI CAATTG 1 cut(s) 621
MhlI GDGCHC 2 cut(s) 290, 439
MlsI TGGCCA 1 cut(s) 615
MluNI TGGCCA 1 cut(s) 615
MlyI GAGTC 3 cut(s) 204, 572, 1486
MmeI TCCRAC 4 cut(s) 1009, 1099, 1292, 1341
MnlI CCTC 5 cut(s) 137, 353, 356, 359, 835
Mox20I TGGCCA 1 cut(s) 615
MroI TCCGGA 1 cut(s) 1058
MscI TGGCCA 1 cut(s) 615
MseI TTAA 7 cut(s) 128, 300, 329, 764, 785, 1302, 1347
MslI CAYNNNNRTG 2 cut(s) 329, 1397
Msp20I TGGCCA 1 cut(s) 615
MspI CCGG 2 cut(s) 68, 1059
MspR9I CCNGG 4 cut(s) 239, 729, 1205, 1225
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 1110
MvaI CCWGG 4 cut(s) 239, 729, 1205, 1225
MwoI GCNNNNNNNGC 5 cut(s) 182, 285, 371, 458, 715
NcoI CCATGG 2 cut(s) 199, 1398
NdeII GATC 3 cut(s) 112, 404, 526
NlaIII CATG 6 cut(s) 203, 831, 894, 1171, 1222, 1402
NlaIV GGNNCC 2 cut(s) 436, 725
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 3 cut(s) 553, 976, 1489
NspI RCATGY 1 cut(s) 831
PciI ACATGT 1 cut(s) 827
PctI GAATGC 1 cut(s) 1110
PfeI GAWTC 2 cut(s) 1022, 1146
PkrI GCNGC 3 cut(s) 519, 697, 717
PleI GAGTC 3 cut(s) 203, 572, 1486
PpsI GAGTC 3 cut(s) 203, 572, 1486
Ppu21I YACGTR 3 cut(s) 208, 1040, 1081
PscI ACATGT 1 cut(s) 827
Psp1406I AACGTT 1 cut(s) 595
Psp6I CCWGG 4 cut(s) 237, 727, 1203, 1223
PspGI CCWGG 4 cut(s) 237, 727, 1203, 1223
PspN4I GGNNCC 2 cut(s) 436, 725
PspPI GGNCC 2 cut(s) 313, 1061
PstI CTGCAG 1 cut(s) 1278
RsaI GTAC 5 cut(s) 27, 206, 725, 1083, 1413
RsaNI GTAC 5 cut(s) 26, 205, 724, 1082, 1412
RseI CAYNNNNRTG 2 cut(s) 329, 1397
SaqAI TTAA 7 cut(s) 128, 300, 329, 764, 785, 1302, 1347
SatI GCNGC 3 cut(s) 518, 696, 716
Sau3AI GATC 3 cut(s) 112, 404, 526
Sau96I GGNCC 2 cut(s) 313, 1061
ScaI AGTACT 1 cut(s) 1413
SchI GAGTC 3 cut(s) 204, 572, 1486
ScrFI CCNGG 4 cut(s) 239, 729, 1205, 1225
SduI GDGCHC 2 cut(s) 290, 439
SfaNI GCATC 3 cut(s) 383, 889, 1339
SfcI CTRYAG 1 cut(s) 1274
SinI GGWCC 2 cut(s) 313, 1061
SmiMI CAYNNNNRTG 2 cut(s) 329, 1397
SnaBI TACGTA 2 cut(s) 1040, 1081
SsiI CCGC 2 cut(s) 489, 628
SspMI CTAG 3 cut(s) 362, 1256, 1388
StyD4I CCNGG 4 cut(s) 237, 727, 1203, 1223
StyI CCWWGG 3 cut(s) 199, 534, 1398
TaaI ACNGT 4 cut(s) 317, 703, 869, 1106
TaiI ACGT 6 cut(s) 56, 210, 598, 1042, 1083, 1323
TaqI TCGA 2 cut(s) 11, 463
TatI WGTACW 1 cut(s) 1411
TfiI GAWTC 2 cut(s) 1022, 1146
Tru1I TTAA 7 cut(s) 128, 300, 329, 764, 785, 1302, 1347
Tru9I TTAA 7 cut(s) 128, 300, 329, 764, 785, 1302, 1347
TscAI CASTG 4 cut(s) 63, 1111, 1362, 1494
TseFI GTSAC 3 cut(s) 553, 976, 1489
TseI GCWGC 3 cut(s) 517, 695, 715
Tsp45I GTSAC 3 cut(s) 553, 976, 1489
TspDTI ATGAA 5 cut(s) 313, 325, 414, 425, 943
TspRI CASTG 4 cut(s) 63, 1111, 1362, 1494
VpaK11BI GGWCC 2 cut(s) 313, 1061
XapI RAATTY 5 cut(s) 811, 967, 1311, 1351, 1463
XbaI TCTAGA 1 cut(s) 1255
XceI RCATGY 1 cut(s) 831
XspI CTAG 3 cut(s) 362, 1256, 1388
ZraI GACGTC 1 cut(s) 1321
ZrmI AGTACT 1 cut(s) 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.