AT5G67620

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014305
Physical Location & Seq
Reverse (-)
26964551 .. 26966010
1460 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67620.1

Sequence Viewer

Length: 549 bp
ATGGGGAATTGTCAAGCGGCCGAAGCAGCGACGGTTTTGATTCATCACCCGGCGGAGAACAAGGTGGAGAGGATTTACTGGTCAGTGACAGCGAGTGACATCATGAAATCCAATCCTGGTCATTACGTTGCGGTGGTGGTGACGTCACCTACGATGAAGAACGAGAAAGGTTTGCCACTGAAGCAGCTTAAACTCCTTCGTCCTGACGACACTTTACTCATCGGACATGTCTATCGTCTCGTCAGCTTCGAAGAGGTTTTGAACGAGTTTGCGACGAAGAAATGTGTGAAGCTTGGCAAGTTATTGAAAGAAGGAGGAGGGCTTGACTTGACGAAGAAGAAGACGAAACACAGGAAGAAGAAGCTGGATCAGGAAACGGGCAAAGTCAACCCGAATTCGGATCCGAATCCGAACCAAGATGGCGCTGATAATGCGGTCGCCGGAGAAAACGGGGGAGATGGGTTTATGAGGAGGAGCCACGGTGGAGGAAGAGGTGGTGGTGGGTGGAGGCCAGCTTTACACAGCATTCCGGAATTTGGATCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

182

Amino Acids

19.71

Weight (kDa)

9.42

Isoelectric Point (pI)

25.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 178 4.7e-27 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67620
fragaria_vesca FvH4_1g17910
malus_domestica MD02G1187500.v1.1 MD15G1298400.v1.1
prunus_persica Prupe.6G219300_v2.0.a1
pyrus_communis pycom02g15250
rosa_chinensis RchiOBHm_Chr2g0108341
rosa_laevigata RLG00000017662
rosa_multiflora Rmu_sc0002372.1_g000015
rosa_roxburghii Rroxscaffold_2G00135230
rosa_rugosa Rorug02G0152200
rosa_samantha Rh2AG201100 Rh2BG214000 Rh2CG205400 Rh2DG208500
rosa_wichuraiana Rw2G016010 Rw2G016070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 146
AccIII TCCGGA 1 cut(s) 529
AciI CCGC 4 cut(s) 17, 53, 131, 434
AclWI GGATC 5 cut(s) 375, 395, 408, 534, 547
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 2 cut(s) 394, 533
AcuI CTGAAG 1 cut(s) 200
AcyI GRCGYC 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 397, 536
AflIII ACRYGT 1 cut(s) 226
AgsI TTSAA 2 cut(s) 262, 307
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 5 cut(s) 187, 246, 292, 364, 515
AluI AGCT 5 cut(s) 187, 246, 292, 364, 515
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 5 cut(s) 375, 395, 408, 534, 547
Aor13HI TCCGGA 1 cut(s) 529
AoxI GGCC 2 cut(s) 18, 509
ApeKI GCWGC 2 cut(s) 26, 184
ApoI RAATTY 2 cut(s) 394, 533
AspLEI GCGC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 3 cut(s) 38, 138, 151
AsuII TTCGAA 1 cut(s) 249
BamHI GGATCC 2 cut(s) 400, 539
BbsI GAAGAC 1 cut(s) 347
BbvI GCAGC 2 cut(s) 38, 196
BccI CCATC 2 cut(s) 413, 452
BciT130I CCWGG 1 cut(s) 117
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 242
BfoI RGCGCY 1 cut(s) 426
BisI GCNGC 3 cut(s) 18, 27, 185
BlsI GCNGC 3 cut(s) 19, 28, 186
Bme1390I CCNGG 2 cut(s) 50, 117
BmiI GGNNCC 3 cut(s) 402, 476, 541
BmrFI CCNGG 2 cut(s) 50, 117
BpiI GAAGAC 1 cut(s) 347
Bpu14I TTCGAA 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 50
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 478
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 2 cut(s) 466, 496
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 536
Bse1I ACTGG 1 cut(s) 83
Bse8I GATNNNNATC 1 cut(s) 405
BseAI TCCGGA 1 cut(s) 529
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 478
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 2 cut(s) 397, 536
BseNI ACTGG 1 cut(s) 83
BseRI GAGGAG 3 cut(s) 330, 484, 487
BseX3I CGGCCG 1 cut(s) 18
BseXI GCAGC 2 cut(s) 38, 196
Bsh1285I CGRYCG 2 cut(s) 21, 438
BshFI GGCC 2 cut(s) 20, 511
BsiEI CGRYCG 2 cut(s) 21, 438
BsiSI CCGG 3 cut(s) 50, 441, 530
BslI CCNNNNNNNGG 2 cut(s) 397, 536
BsmAI GTCTC 1 cut(s) 242
BsmBI CGTCTC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 525
BsnI GGCC 2 cut(s) 20, 511
Bsp119I TTCGAA 1 cut(s) 249
Bsp13I TCCGGA 1 cut(s) 529
Bsp143I GATC 3 cut(s) 367, 400, 539
BspACI CCGC 4 cut(s) 17, 53, 131, 434
BspANI GGCC 2 cut(s) 20, 511
BspEI TCCGGA 1 cut(s) 529
BspHI TCATGA 2 cut(s) 102, 545
BspLI GGNNCC 3 cut(s) 402, 476, 541
BspPI GGATC 5 cut(s) 375, 395, 408, 534, 547
BspT104I TTCGAA 1 cut(s) 249
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 3 cut(s) 367, 400, 539
BssNI GRCGYC 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 34, 482
Bst6I CTCTTC 2 cut(s) 246, 484
BstACI GRCGYC 1 cut(s) 143
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 478
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 3 cut(s) 370, 403, 542
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 3 cut(s) 367, 400, 539
BstMCI CGRYCG 2 cut(s) 21, 438
BstMWI GCNNNNNNNGC 4 cut(s) 23, 26, 181, 431
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 48, 115
BstV1I GCAGC 2 cut(s) 38, 196
BstV2I GAAGAC 1 cut(s) 347
BstX2I RGATCY 2 cut(s) 400, 539
BstYI RGATCY 2 cut(s) 400, 539
BstZI CGGCCG 1 cut(s) 18
BsuRI GGCC 2 cut(s) 20, 511
BtgI CCRYGG 1 cut(s) 478
BtsIMutI CAGTG 2 cut(s) 90, 176
Cac8I GCNNGC 1 cut(s) 513
CciI TCATGA 2 cut(s) 102, 545
CfoI GCGC 1 cut(s) 425
CviAII CATG 3 cut(s) 103, 227, 546
CviJI RGCY 9 cut(s) 20, 187, 246, 292, 322, 364, 477, 511, 515
CviKI_1 RGCY 9 cut(s) 20, 187, 246, 292, 322, 364, 477, 511, 515
DpnI GATC 3 cut(s) 369, 402, 541
DpnII GATC 3 cut(s) 367, 400, 539
EaeI YGGCCR 1 cut(s) 18
EagI CGGCCG 1 cut(s) 18
Eam1104I CTCTTC 2 cut(s) 246, 484
EarI CTCTTC 2 cut(s) 246, 484
EciI GGCGGA 1 cut(s) 68
EclXI CGGCCG 1 cut(s) 18
Eco52I CGGCCG 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 115
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 3 cut(s) 106, 230, 549
FaiI YATR 4 cut(s) 104, 228, 467, 547
FatI CATG 3 cut(s) 102, 226, 545
Fnu4HI GCNGC 3 cut(s) 18, 27, 185
Fsp4HI GCNGC 3 cut(s) 18, 27, 185
GlaI GCGC 1 cut(s) 424
GluI GCNGC 3 cut(s) 18, 27, 185
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 2 cut(s) 20, 511
HapII CCGG 3 cut(s) 50, 441, 530
HhaI GCGC 1 cut(s) 425
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 3 cut(s) 106, 230, 549
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 2 cut(s) 40, 406
HpaII CCGG 3 cut(s) 50, 441, 530
HphI GGTGA 3 cut(s) 38, 138, 151
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 4 cut(s) 224, 400, 405, 411
Hpy188III TCNNGA 5 cut(s) 103, 203, 371, 530, 546
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 2 cut(s) 34, 277
HpyAV CCTTC 2 cut(s) 206, 305
HpyCH4III ACNGT 2 cut(s) 34, 482
HpyCH4IV ACGT 2 cut(s) 126, 143
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 26, 181, 431
HpySE526I ACGT 2 cut(s) 126, 143
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 3 cut(s) 106, 230, 549
HspAI GCGC 1 cut(s) 423
Kpn2I TCCGGA 1 cut(s) 529
Kzo9I GATC 3 cut(s) 367, 400, 539
LmnI GCTCC 1 cut(s) 474
Lsp1109I GCAGC 2 cut(s) 38, 196
MaeII ACGT 2 cut(s) 126, 143
MaeIII GTNAC 4 cut(s) 85, 95, 139, 144
MalI GATC 3 cut(s) 369, 402, 541
MboI GATC 3 cut(s) 367, 400, 539
MboII GAAGA 9 cut(s) 169, 263, 289, 346, 349, 352, 367, 370, 501
MflI RGATCY 2 cut(s) 400, 539
MluCI AATT 3 cut(s) 7, 394, 533
MnlI CCTC 9 cut(s) 63, 247, 308, 311, 462, 465, 479, 485, 501
MroI TCCGGA 1 cut(s) 529
MseI TTAA 1 cut(s) 189
MspI CCGG 3 cut(s) 50, 441, 530
MspR9I CCNGG 2 cut(s) 50, 117
Mva1269I GAATGC 1 cut(s) 525
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 4 cut(s) 23, 26, 181, 431
NciI CCSGG 1 cut(s) 50
NdeII GATC 3 cut(s) 367, 400, 539
NlaIII CATG 3 cut(s) 106, 230, 549
NlaIV GGNNCC 3 cut(s) 402, 476, 541
NmuCI GTSAC 4 cut(s) 85, 95, 139, 144
NspI RCATGY 1 cut(s) 230
NspV TTCGAA 1 cut(s) 249
PagI TCATGA 2 cut(s) 102, 545
PciI ACATGT 1 cut(s) 226
PcsI WCGNNNNNNNCGW 2 cut(s) 149, 246
PctI GAATGC 1 cut(s) 525
PfeI GAWTC 2 cut(s) 40, 406
PkrI GCNGC 3 cut(s) 19, 28, 186
PscI ACATGT 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 402, 476, 541
PsuI RGATCY 2 cut(s) 400, 539
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 3 cut(s) 18, 27, 185
Sau3AI GATC 3 cut(s) 367, 400, 539
ScrFI CCNGG 2 cut(s) 50, 117
SfuI TTCGAA 1 cut(s) 249
Sse9I AATT 3 cut(s) 7, 394, 533
SsiI CCGC 4 cut(s) 17, 53, 131, 434
StyD4I CCNGG 2 cut(s) 48, 115
TaaI ACNGT 2 cut(s) 34, 482
TaiI ACGT 2 cut(s) 129, 146
TaqI TCGA 1 cut(s) 249
TasI AATT 3 cut(s) 7, 394, 533
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 2 cut(s) 40, 406
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 2 cut(s) 90, 183
TseFI GTSAC 4 cut(s) 85, 95, 139, 144
TseI GCWGC 2 cut(s) 26, 184
Tsp45I GTSAC 4 cut(s) 85, 95, 139, 144
TspDTI ATGAA 3 cut(s) 32, 119, 170
TspRI CASTG 2 cut(s) 90, 183
XapI RAATTY 2 cut(s) 394, 533
XceI RCATGY 1 cut(s) 230
ZraI GACGTC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.