MD02G1187500.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014305
Physical Location & Seq
Reverse (-)
17091112 .. 17094646
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1187500.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGGGAATTGTCAGGCGGCAGAGGCAGCGACGGTGGTGATTCAGCACCCGGGGAACAACAAGATCGAGAGGATTTACTGGTCTGTGGGCGCTCATGAGGTCATGACTTCCAACCCCGGCCACTACGTAGCTCTTGTCGTCAACTCTCCGACCATGAAGTCTGAGACTGGTGCTCCAGTGAAGCAGCTCAAGCTTCTCAGGCCTGACGACACTTTGCTCATTGGACAGGTTTATCGCCTCATCAGTTTCGAAGATGTGTTGAAGGAGTTTGCTGCTAAAAAATCTGTGAAGCTGGGGAAGTTGCTGAAACATAGAGGTGAGCTTGGAGTGGAAATGAAGATGAAGGACTTGGCAGGTCAGAATTTGAAGTCCGTCAACAACAATTCCATCAAGATGGAGATTGTGGGAAGCAACGGTGGCAGCACAAGCACTAACAACGGCAGAATCTACAGAAGCGTGGGAAGAAATTATGGTGGTGGTGGTGGAGATGGAAGTGGTGTGGGGCAGTGGAGGCCAGCCTTGCAGAGTATTGCGGAGATTGGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

183

Amino Acids

19.48

Weight (kDa)

9.57

Isoelectric Point (pI)

24.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 179 4.2e-26 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67620
fragaria_vesca FvH4_1g17910
malus_domestica MD02G1187500.v1.1 MD15G1298400.v1.1
prunus_persica Prupe.6G219300_v2.0.a1
pyrus_communis pycom02g15250
rosa_chinensis RchiOBHm_Chr2g0108341
rosa_laevigata RLG00000017662
rosa_multiflora Rmu_sc0002372.1_g000015
rosa_roxburghii Rroxscaffold_2G00135230
rosa_rugosa Rorug02G0152200
rosa_samantha Rh2AG201100 Rh2BG214000 Rh2CG205400 Rh2DG208500
rosa_wichuraiana Rw2G016010 Rw2G016070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 344
AciI CCGC 2 cut(s) 17, 533
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 1 cut(s) 361
AgsI TTSAA 2 cut(s) 262, 367
AluBI AGCT 5 cut(s) 131, 187, 193, 292, 322
AluI AGCT 5 cut(s) 131, 187, 193, 292, 322
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 3 cut(s) 118, 200, 512
ApeKI GCWGC 4 cut(s) 26, 184, 272, 420
ApoI RAATTY 1 cut(s) 361
AspLEI GCGC 1 cut(s) 92
AsuC2I CCSGG 3 cut(s) 50, 51, 117
AsuHPI GGTGA 2 cut(s) 49, 329
AsuII TTCGAA 1 cut(s) 249
AvaI CYCGRG 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 4 cut(s) 38, 196, 259, 432
BccI CCATC 3 cut(s) 388, 395, 482
BceAI ACGGC 1 cut(s) 454
BcnI CCSGG 3 cut(s) 50, 51, 117
BcoDI GTCTC 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 448
BfoI RGCGCY 1 cut(s) 93
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 5 cut(s) 18, 27, 185, 273, 421
BlsI GCNGC 5 cut(s) 19, 28, 186, 274, 422
Bme1390I CCNGG 3 cut(s) 50, 51, 117
BmeT110I CYCGRG 1 cut(s) 49
BmrFI CCNGG 3 cut(s) 50, 51, 117
BpmI CTGGAG 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 3 cut(s) 50, 51, 117
BsaAI YACGTR 1 cut(s) 127
BsaJI CCNNGG 3 cut(s) 49, 50, 115
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bse1I ACTGG 3 cut(s) 83, 172, 176
BseDI CCNNGG 3 cut(s) 49, 50, 115
BseMII CTCAG 2 cut(s) 153, 211
BseNI ACTGG 3 cut(s) 83, 172, 176
BseXI GCAGC 4 cut(s) 38, 196, 259, 432
BseYI CCCAGC 1 cut(s) 292
BshFI GGCC 3 cut(s) 120, 202, 514
BsiHKAI GWGCWC 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 2 cut(s) 50, 117
BsmAI GTCTC 1 cut(s) 158
BsnI GGCC 3 cut(s) 120, 202, 514
BsoBI CYCGRG 1 cut(s) 49
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 2 cut(s) 17, 533
BspANI GGCC 3 cut(s) 120, 202, 514
BspCNI CTCAG 2 cut(s) 154, 210
BspHI TCATGA 2 cut(s) 94, 102
BspMI ACCTGC 1 cut(s) 344
BspT104I TTCGAA 1 cut(s) 249
BsrI ACTGG 3 cut(s) 83, 172, 176
BssECI CCNNGG 3 cut(s) 49, 50, 115
BssMI GATC 1 cut(s) 63
Bst4CI ACNGT 2 cut(s) 34, 416
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 2 cut(s) 162, 197
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 8 cut(s) 23, 26, 190, 199, 417, 426, 511, 520
BstSCI CCNGG 3 cut(s) 48, 49, 115
BstSFI CTRYAG 1 cut(s) 448
BstSNI TACGTA 1 cut(s) 127
BstV1I GCAGC 4 cut(s) 38, 196, 259, 432
BstXI CCANNNNNNTGG 1 cut(s) 394
BsuRI GGCC 3 cut(s) 120, 202, 514
BtsI GCAGTG 1 cut(s) 512
BtsIMutI CAGTG 2 cut(s) 183, 512
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 1 cut(s) 516
CciI TCATGA 2 cut(s) 94, 102
CfoI GCGC 1 cut(s) 92
Cfr9I CCCGGG 1 cut(s) 49
CviAII CATG 3 cut(s) 95, 103, 154
CviJI RGCY 9 cut(s) 120, 131, 187, 193, 202, 292, 322, 514, 518
CviKI_1 RGCY 9 cut(s) 120, 131, 187, 193, 202, 292, 322, 514, 518
DdeI CTNAG 2 cut(s) 162, 197
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
DrdI GACNNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 118
Eco105I TACGTA 1 cut(s) 127
Eco147I AGGCCT 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 49
FaeI CATG 3 cut(s) 98, 106, 157
FaiI YATR 5 cut(s) 96, 104, 155, 312, 471
FatI CATG 3 cut(s) 94, 102, 153
Fnu4HI GCNGC 5 cut(s) 18, 27, 185, 273, 421
Fsp4HI GCNGC 5 cut(s) 18, 27, 185, 273, 421
GlaI GCGC 1 cut(s) 91
GluI GCNGC 5 cut(s) 18, 27, 185, 273, 421
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 3 cut(s) 120, 202, 514
HapII CCGG 2 cut(s) 50, 117
HhaI GCGC 1 cut(s) 92
Hin1II CATG 3 cut(s) 98, 106, 157
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 2 cut(s) 142, 376
HindII GTYRAC 2 cut(s) 142, 376
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 2 cut(s) 40, 444
HpaII CCGG 2 cut(s) 50, 117
HphI GGTGA 2 cut(s) 49, 329
Hpy166II GTNNAC 2 cut(s) 142, 376
Hpy188I TCNGA 3 cut(s) 150, 163, 360
Hpy188III TCNNGA 4 cut(s) 67, 95, 103, 391
Hpy8I GTNNAC 2 cut(s) 142, 376
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 2 cut(s) 256, 337
HpyCH4III ACNGT 2 cut(s) 34, 416
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 1 cut(s) 523
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 26, 190, 199, 417, 426, 511, 520
HpyF3I CTNAG 2 cut(s) 162, 197
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 3 cut(s) 98, 106, 157
HspAI GCGC 1 cut(s) 90
Kzo9I GATC 1 cut(s) 63
LmnI GCTCC 1 cut(s) 178
Lsp1109I GCAGC 4 cut(s) 38, 196, 259, 432
MaeII ACGT 1 cut(s) 126
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 3 cut(s) 263, 349, 474
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 4 cut(s) 7, 361, 382, 466
MmeI TCCRAC 2 cut(s) 135, 173
MnlI CCTC 6 cut(s) 16, 63, 91, 248, 308, 504
MslI CAYNNNNRTG 2 cut(s) 315, 392
MspI CCGG 2 cut(s) 50, 117
MspR9I CCNGG 3 cut(s) 50, 51, 117
MwoI GCNNNNNNNGC 8 cut(s) 23, 26, 190, 199, 417, 426, 511, 520
NciI CCSGG 3 cut(s) 50, 51, 117
NdeII GATC 1 cut(s) 63
NlaIII CATG 3 cut(s) 98, 106, 157
NspV TTCGAA 1 cut(s) 249
PagI TCATGA 2 cut(s) 94, 102
PceI AGGCCT 1 cut(s) 202
PfeI GAWTC 2 cut(s) 40, 444
PkrI GCNGC 5 cut(s) 19, 28, 186, 274, 422
Ppu21I YACGTR 1 cut(s) 127
PspFI CCCAGC 1 cut(s) 292
RseI CAYNNNNRTG 2 cut(s) 315, 392
SatI GCNGC 5 cut(s) 18, 27, 185, 273, 421
Sau3AI GATC 1 cut(s) 63
ScrFI CCNGG 3 cut(s) 50, 51, 117
SduI GDGCHC 1 cut(s) 175
SfcI CTRYAG 1 cut(s) 448
SfuI TTCGAA 1 cut(s) 249
SmaI CCCGGG 1 cut(s) 51
SmiMI CAYNNNNRTG 2 cut(s) 315, 392
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SnaBI TACGTA 1 cut(s) 127
Sse9I AATT 4 cut(s) 7, 361, 382, 466
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 2 cut(s) 17, 533
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 3 cut(s) 48, 49, 115
TaaI ACNGT 2 cut(s) 34, 416
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 66, 249
TasI AATT 4 cut(s) 7, 361, 382, 466
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 2 cut(s) 40, 444
TscAI CASTG 2 cut(s) 183, 512
TseI GCWGC 4 cut(s) 26, 184, 272, 420
TspDTI ATGAA 3 cut(s) 170, 350, 356
TspGWI ACGGA 1 cut(s) 361
TspMI CCCGGG 1 cut(s) 49
TspRI CASTG 2 cut(s) 183, 512
XapI RAATTY 1 cut(s) 361
XmaI CCCGGG 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.