pycom02g15250

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014305
Physical Location & Seq
Reverse (-)
11928892 .. 11931851
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15250.1

Sequence Viewer

Length: 552 bp
ATGGGGAATTGTCAGGCGGCAGAGGCAGCGACGGTGGTGATTCAGCATCCGGGGAACAACAAGATCGAGAGGATTTACTGGTCTGTGGGCGCTCATGAGGTCATGACTTCCAACCCCGGCCACTACGTGGCTCTTGTCGTCAACTCTCCGACCATGAAGTCTGTGACTGGTGCTCCAGTGATCAAGCAGCTCAAGCTTCTCAGGCCTGACGACACTTTGCTCATTGGACAGGTTTATCGCTTCATCAGTTTCGAAGATGTGTTGAAGGAGTTTGCTGCTAAAAAATCTGTGAAGCTGGGGAAGTTGCTGAAGCATAGAGGTGAGCTTGGAGTGGAAATGAAGATGAAGGACTTGGCAGGTCAGAATTTGAAGTCCGTCAACAACAATTCCATCAAGATGGAGAGTGTGGGAAGCAACGGTGGCAGCACAAGCACTAACAACGGCAGAACCTACAGAAGCGTGGGAAGAAATTATGGTGGTGGTGGTGGAGGTGGAGGTGGTGTGGGGCAGTGGAGGCCAGCCTTGCAGAGTATTGCAGAGGTTGGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

184

Amino Acids

19.46

Weight (kDa)

9.78

Isoelectric Point (pI)

24.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 180 1.2e-25 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67620
fragaria_vesca FvH4_1g17910
malus_domestica MD02G1187500.v1.1 MD15G1298400.v1.1
prunus_persica Prupe.6G219300_v2.0.a1
pyrus_communis pycom02g15250
rosa_chinensis RchiOBHm_Chr2g0108341
rosa_laevigata RLG00000017662
rosa_multiflora Rmu_sc0002372.1_g000015
rosa_roxburghii Rroxscaffold_2G00135230
rosa_rugosa Rorug02G0152200
rosa_samantha Rh2AG201100 Rh2BG214000 Rh2CG205400 Rh2DG208500
rosa_wichuraiana Rw2G016010 Rw2G016070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 127
AciI CCGC 1 cut(s) 17
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 1 cut(s) 329
AdeI CACNNNGTG 1 cut(s) 127
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 2 cut(s) 265, 370
AluBI AGCT 4 cut(s) 190, 196, 295, 325
AluI AGCT 4 cut(s) 190, 196, 295, 325
Alw21I GWGCWC 1 cut(s) 175
AoxI GGCC 3 cut(s) 118, 203, 515
ApeKI GCWGC 4 cut(s) 26, 187, 275, 423
ApoI RAATTY 1 cut(s) 364
AspLEI GCGC 1 cut(s) 92
AsuC2I CCSGG 2 cut(s) 51, 117
AsuHPI GGTGA 2 cut(s) 49, 332
AsuII TTCGAA 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 4 cut(s) 38, 199, 262, 435
BccI CCATC 2 cut(s) 391, 398
BceAI ACGGC 1 cut(s) 457
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 2 cut(s) 51, 117
BfmI CTRYAG 1 cut(s) 451
BfoI RGCGCY 1 cut(s) 93
BfuAI ACCTGC 1 cut(s) 347
BisI GCNGC 5 cut(s) 18, 27, 188, 276, 424
BlsI GCNGC 5 cut(s) 19, 28, 189, 277, 425
Bme1390I CCNGG 2 cut(s) 51, 117
BmrFI CCNGG 2 cut(s) 51, 117
BmsI GCATC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 2 cut(s) 51, 117
BsaAI YACGTR 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 50, 115
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 486, 516
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse1I ACTGG 3 cut(s) 83, 172, 176
BseDI CCNNGG 2 cut(s) 50, 115
BseGI GGATG 1 cut(s) 46
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 214
BseNI ACTGG 3 cut(s) 83, 172, 176
BseXI GCAGC 4 cut(s) 38, 199, 262, 435
BseYI CCCAGC 1 cut(s) 295
BshFI GGCC 3 cut(s) 120, 205, 517
BsiHKAI GWGCWC 1 cut(s) 175
BsiSI CCGG 2 cut(s) 50, 117
BslI CCNNNNNNNGG 1 cut(s) 127
BsnI GGCC 3 cut(s) 120, 205, 517
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 2 cut(s) 63, 180
BspACI CCGC 1 cut(s) 17
BspANI GGCC 3 cut(s) 120, 205, 517
BspCNI CTCAG 1 cut(s) 213
BspHI TCATGA 2 cut(s) 94, 102
BspMI ACCTGC 1 cut(s) 347
BspT104I TTCGAA 1 cut(s) 252
BsrI ACTGG 3 cut(s) 83, 172, 176
BssECI CCNNGG 2 cut(s) 50, 115
BssMI GATC 2 cut(s) 63, 180
Bst4CI ACNGT 2 cut(s) 34, 419
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 2 cut(s) 66, 183
BstMBI GATC 2 cut(s) 63, 180
BstMWI GCNNNNNNNGC 8 cut(s) 23, 26, 193, 202, 420, 429, 514, 523
BstSCI CCNGG 2 cut(s) 49, 115
BstSFI CTRYAG 1 cut(s) 451
BstV1I GCAGC 4 cut(s) 38, 199, 262, 435
BstXI CCANNNNNNTGG 1 cut(s) 397
BsuRI GGCC 3 cut(s) 120, 205, 517
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 2 cut(s) 183, 515
BveI ACCTGC 1 cut(s) 347
Cac8I GCNNGC 1 cut(s) 519
CciI TCATGA 2 cut(s) 94, 102
CfoI GCGC 1 cut(s) 92
CviAII CATG 3 cut(s) 95, 103, 154
CviJI RGCY 9 cut(s) 120, 131, 190, 196, 205, 295, 325, 517, 521
CviKI_1 RGCY 9 cut(s) 120, 131, 190, 196, 205, 295, 325, 517, 521
DdeI CTNAG 1 cut(s) 200
DpnI GATC 2 cut(s) 65, 182
DpnII GATC 2 cut(s) 63, 180
DraIII CACNNNGTG 1 cut(s) 127
DrdI GACNNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 118
Eco147I AGGCCT 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 329
FaeI CATG 3 cut(s) 98, 106, 157
FaiI YATR 5 cut(s) 96, 104, 155, 315, 474
FatI CATG 3 cut(s) 94, 102, 153
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 5 cut(s) 18, 27, 188, 276, 424
FokI GGATG 1 cut(s) 33
Fsp4HI GCNGC 5 cut(s) 18, 27, 188, 276, 424
GlaI GCGC 1 cut(s) 91
GluI GCNGC 5 cut(s) 18, 27, 188, 276, 424
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 3 cut(s) 120, 205, 517
HapII CCGG 2 cut(s) 50, 117
HhaI GCGC 1 cut(s) 92
Hin1II CATG 3 cut(s) 98, 106, 157
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 2 cut(s) 142, 379
HindII GTYRAC 2 cut(s) 142, 379
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 1 cut(s) 40
HpaII CCGG 2 cut(s) 50, 117
HphI GGTGA 2 cut(s) 49, 332
Hpy166II GTNNAC 2 cut(s) 142, 379
Hpy188I TCNGA 2 cut(s) 150, 363
Hpy188III TCNNGA 4 cut(s) 67, 95, 103, 394
Hpy8I GTNNAC 2 cut(s) 142, 379
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 2 cut(s) 259, 340
HpyCH4III ACNGT 2 cut(s) 34, 419
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 526, 536
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 26, 193, 202, 420, 429, 514, 523
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 3 cut(s) 98, 106, 157
HspAI GCGC 1 cut(s) 90
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 63, 180
LmnI GCTCC 1 cut(s) 178
Lsp1109I GCAGC 4 cut(s) 38, 199, 262, 435
LweI GCATC 1 cut(s) 55
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 163
MalI GATC 2 cut(s) 65, 182
MboI GATC 2 cut(s) 63, 180
MboII GAAGA 3 cut(s) 266, 352, 477
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 4 cut(s) 7, 364, 385, 469
MmeI TCCRAC 3 cut(s) 135, 173, 523
MnlI CCTC 8 cut(s) 16, 63, 91, 311, 482, 488, 507, 532
MslI CAYNNNNRTG 2 cut(s) 318, 395
MspI CCGG 2 cut(s) 50, 117
MspR9I CCNGG 2 cut(s) 51, 117
MwoI GCNNNNNNNGC 8 cut(s) 23, 26, 193, 202, 420, 429, 514, 523
NciI CCSGG 2 cut(s) 51, 117
NdeII GATC 2 cut(s) 63, 180
NlaIII CATG 3 cut(s) 98, 106, 157
NmuCI GTSAC 1 cut(s) 163
NspV TTCGAA 1 cut(s) 252
PagI TCATGA 2 cut(s) 94, 102
PceI AGGCCT 1 cut(s) 205
PfeI GAWTC 1 cut(s) 40
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 5 cut(s) 19, 28, 189, 277, 425
Ppu21I YACGTR 1 cut(s) 127
PspFI CCCAGC 1 cut(s) 295
RseI CAYNNNNRTG 2 cut(s) 318, 395
SatI GCNGC 5 cut(s) 18, 27, 188, 276, 424
Sau3AI GATC 2 cut(s) 63, 180
ScrFI CCNGG 2 cut(s) 51, 117
SduI GDGCHC 1 cut(s) 175
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 451
SfuI TTCGAA 1 cut(s) 252
SmiMI CAYNNNNRTG 2 cut(s) 318, 395
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 4 cut(s) 7, 364, 385, 469
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 49, 115
TaaI ACNGT 2 cut(s) 34, 419
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 66, 252
TasI AATT 4 cut(s) 7, 364, 385, 469
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 1 cut(s) 40
TscAI CASTG 2 cut(s) 183, 515
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 4 cut(s) 26, 187, 275, 423
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 4 cut(s) 170, 232, 353, 359
TspGWI ACGGA 1 cut(s) 364
TspRI CASTG 2 cut(s) 183, 515
Van91I CCANNNNNTGG 1 cut(s) 127
XapI RAATTY 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.