Rh2DG208500

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014305
Physical Location & Seq
Reverse (-)
19617633 .. 19622157
4525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG208500.1

Sequence Viewer

Length: 408 bp
ATGGGCAACTGTCAGGCGGCGGAGGCGGCGACGGTGATTATTCACCACCCGGCCAACAACAAGGTCGAGAGGATTTACTGGTCGGTGAGCGCTCACGAGATCATGACGTCGAATCCGGGGAACTACGTGGCGCTTGTGGTGACGTCGCCGACGTTGAAGGCCGAGAATGGCGCGCCGGTTAAGCAGCTCAAGCTTCTCAGGCCGGACGACACTTTGCTTATCGGACAGGTTTACCGGCTCATCAGTTTCGAAGATGTGTTGAAGGAGTTTGCTGCTAAAAAGTCTGTGAAGCTGGGGAGGTTGCTGAGGGATAGCGGTGGCCTTGGCCTGGAACTGAAGAAAAAAGACTTGCATGGTCCGAATCCGAATTCGAAGTCCGACGACCGCAATTCTACCAAGGTAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

135

Amino Acids

14.77

Weight (kDa)

9.41

Isoelectric Point (pI)

14.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 132 3.1e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67620
fragaria_vesca FvH4_1g17910
malus_domestica MD02G1187500.v1.1 MD15G1298400.v1.1
prunus_persica Prupe.6G219300_v2.0.a1
pyrus_communis pycom02g15250
rosa_chinensis RchiOBHm_Chr2g0108341
rosa_laevigata RLG00000017662
rosa_multiflora Rmu_sc0002372.1_g000015
rosa_roxburghii Rroxscaffold_2G00135230
rosa_rugosa Rorug02G0152200
rosa_samantha Rh2AG201100 Rh2BG214000 Rh2CG205400 Rh2DG208500
rosa_wichuraiana Rw2G016010 Rw2G016070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 110, 146
AccII CGCG 1 cut(s) 173
AciI CCGC 5 cut(s) 17, 20, 26, 315, 385
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 2 cut(s) 107, 143
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 2 cut(s) 157, 262
AjnI CCWGG 1 cut(s) 327
AluBI AGCT 3 cut(s) 187, 193, 292
AluI AGCT 3 cut(s) 187, 193, 292
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 5 cut(s) 51, 159, 200, 319, 325
ApeKI GCWGC 2 cut(s) 184, 272
ApoI RAATTY 1 cut(s) 367
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
AscI GGCGCGCC 1 cut(s) 171
AspLEI GCGC 4 cut(s) 92, 133, 173, 175
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 2 cut(s) 50, 117
AsuHPI GGTGA 4 cut(s) 35, 46, 97, 151
AsuII TTCGAA 2 cut(s) 249, 371
AvaII GGWCC 1 cut(s) 356
BauI CACGAG 1 cut(s) 95
BbvCI CCTCAGC 1 cut(s) 305
BbvI GCAGC 2 cut(s) 196, 259
BciT130I CCWGG 1 cut(s) 329
BcnI CCSGG 2 cut(s) 50, 117
BfoI RGCGCY 2 cut(s) 93, 134
BisI GCNGC 4 cut(s) 18, 27, 185, 273
BlsI GCNGC 4 cut(s) 19, 28, 186, 274
Bme1390I CCNGG 3 cut(s) 50, 117, 329
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmrFI CCNGG 3 cut(s) 50, 117, 329
Bpu10I CCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 2 cut(s) 249, 371
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 2 cut(s) 50, 117
BsaAI YACGTR 1 cut(s) 127
BsaHI GRCGYC 2 cut(s) 107, 143
BsaJI CCNNGG 3 cut(s) 116, 322, 396
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse118I RCCGGY 2 cut(s) 175, 234
Bse1I ACTGG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 3 cut(s) 116, 322, 396
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMII CTCAG 2 cut(s) 211, 296
BseNI ACTGG 1 cut(s) 83
BsePI GCGCGC 1 cut(s) 171
BseXI GCAGC 2 cut(s) 196, 259
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 385
BshFI GGCC 5 cut(s) 53, 161, 202, 321, 327
BsiEI CGRYCG 1 cut(s) 385
BsiSI CCGG 5 cut(s) 50, 116, 176, 203, 235
BslI CCNNNNNNNGG 1 cut(s) 328
BsnI GGCC 5 cut(s) 53, 161, 202, 321, 327
Bsp119I TTCGAA 2 cut(s) 249, 371
Bsp143I GATC 1 cut(s) 99
BspACI CCGC 5 cut(s) 17, 20, 26, 315, 385
BspANI GGCC 5 cut(s) 53, 161, 202, 321, 327
BspCNI CTCAG 2 cut(s) 210, 297
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 102
BspT104I TTCGAA 2 cut(s) 249, 371
BsrFI RCCGGY 2 cut(s) 175, 234
BsrI ACTGG 1 cut(s) 83
BssAI RCCGGY 2 cut(s) 175, 234
BssECI CCNNGG 3 cut(s) 116, 322, 396
BssHII GCGCGC 1 cut(s) 171
BssMI GATC 1 cut(s) 99
BssNI GRCGYC 2 cut(s) 107, 143
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 2 cut(s) 322, 396
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 2 cut(s) 11, 34
BstACI GRCGYC 2 cut(s) 107, 143
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 2 cut(s) 249, 371
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 2 cut(s) 197, 305
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 2 cut(s) 93, 134
BstHHI GCGC 4 cut(s) 92, 133, 173, 175
BstKTI GATC 1 cut(s) 102
BstMBI GATC 1 cut(s) 99
BstMCI CGRYCG 1 cut(s) 385
BstMWI GCNNNNNNNGC 5 cut(s) 23, 26, 181, 190, 199
BstNI CCWGG 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 48, 115, 327
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 2 cut(s) 196, 259
BsuRI GGCC 5 cut(s) 53, 161, 202, 321, 327
Cac8I GCNNGC 1 cut(s) 173
CciI TCATGA 1 cut(s) 102
CfoI GCGC 4 cut(s) 92, 133, 173, 175
Cfr10I RCCGGY 2 cut(s) 175, 234
Cfr13I GGNCC 1 cut(s) 356
CviAII CATG 2 cut(s) 103, 353
CviJI RGCY 9 cut(s) 53, 161, 187, 193, 202, 238, 292, 321, 327
CviKI_1 RGCY 9 cut(s) 53, 161, 187, 193, 202, 238, 292, 321, 327
DdeI CTNAG 2 cut(s) 197, 305
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 51
EciI GGCGGA 1 cut(s) 35
Eco130I CCWWGG 2 cut(s) 322, 396
Eco47I GGWCC 1 cut(s) 356
Eco47III AGCGCT 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 356
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 327
EcoT14I CCWWGG 2 cut(s) 322, 396
ErhI CCWWGG 2 cut(s) 322, 396
FaeI CATG 2 cut(s) 106, 356
FaiI YATR 3 cut(s) 104, 354, 406
FatI CATG 2 cut(s) 102, 352
Fnu4HI GCNGC 4 cut(s) 18, 27, 185, 273
Fsp4HI GCNGC 4 cut(s) 18, 27, 185, 273
GlaI GCGC 4 cut(s) 91, 132, 172, 174
GluI GCNGC 4 cut(s) 18, 27, 185, 273
GsaI CCCAGC 1 cut(s) 296
HaeII RGCGCY 2 cut(s) 93, 134
HaeIII GGCC 5 cut(s) 53, 161, 202, 321, 327
HapII CCGG 5 cut(s) 50, 116, 176, 203, 235
HhaI GCGC 4 cut(s) 92, 133, 173, 175
Hin1I GRCGYC 2 cut(s) 107, 143
Hin1II CATG 2 cut(s) 106, 356
Hin6I GCGC 4 cut(s) 90, 131, 171, 173
HinP1I GCGC 4 cut(s) 90, 131, 171, 173
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 2 cut(s) 112, 361
HpaII CCGG 5 cut(s) 50, 116, 176, 203, 235
HphI GGTGA 4 cut(s) 35, 46, 97, 151
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 4 cut(s) 224, 360, 366, 379
Hpy188III TCNNGA 3 cut(s) 67, 95, 103
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 5 cut(s) 34, 112, 148, 154, 383
HpyAV CCTTC 2 cut(s) 151, 256
HpyCH4III ACNGT 2 cut(s) 11, 34
HpyCH4IV ACGT 4 cut(s) 107, 126, 143, 152
HpyCH4V TGCA 1 cut(s) 352
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 26, 181, 190, 199
HpyF3I CTNAG 2 cut(s) 197, 305
HpySE526I ACGT 4 cut(s) 107, 126, 143, 152
Hsp92I GRCGYC 2 cut(s) 107, 143
Hsp92II CATG 2 cut(s) 106, 356
HspAI GCGC 4 cut(s) 90, 131, 171, 173
Kzo9I GATC 1 cut(s) 99
Lsp1109I GCAGC 2 cut(s) 196, 259
MaeII ACGT 4 cut(s) 107, 126, 143, 152
MaeIII GTNAC 1 cut(s) 139
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MboII GAAGA 2 cut(s) 263, 349
MluCI AATT 2 cut(s) 367, 388
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 4 cut(s) 16, 63, 291, 300
MseI TTAA 1 cut(s) 180
MspI CCGG 5 cut(s) 50, 116, 176, 203, 235
MspR9I CCNGG 3 cut(s) 50, 117, 329
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 5 cut(s) 23, 26, 181, 190, 199
NciI CCSGG 2 cut(s) 50, 117
NdeII GATC 1 cut(s) 99
NlaIII CATG 2 cut(s) 106, 356
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 139
NspV TTCGAA 2 cut(s) 249, 371
PagI TCATGA 1 cut(s) 102
PalAI GGCGCGCC 1 cut(s) 171
PauI GCGCGC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PfeI GAWTC 2 cut(s) 112, 361
PkrI GCNGC 4 cut(s) 19, 28, 186, 274
Ppu21I YACGTR 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 327
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 1 cut(s) 327
PspPI GGNCC 1 cut(s) 356
PteI GCGCGC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 4 cut(s) 18, 27, 185, 273
Sau3AI GATC 1 cut(s) 99
Sau96I GGNCC 1 cut(s) 356
ScrFI CCNGG 3 cut(s) 50, 117, 329
SfuI TTCGAA 2 cut(s) 249, 371
SgsI GGCGCGCC 1 cut(s) 171
SinI GGWCC 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 2 cut(s) 367, 388
SsiI CCGC 5 cut(s) 17, 20, 26, 315, 385
StyD4I CCNGG 3 cut(s) 48, 115, 327
StyI CCWWGG 2 cut(s) 322, 396
TaaI ACNGT 2 cut(s) 11, 34
TaiI ACGT 4 cut(s) 110, 129, 146, 155
TaqI TCGA 4 cut(s) 66, 110, 249, 371
TasI AATT 2 cut(s) 367, 388
TauI GCSGC 2 cut(s) 20, 29
TfiI GAWTC 2 cut(s) 112, 361
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 2 cut(s) 184, 272
Tsp45I GTSAC 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 1 cut(s) 367
ZraI GACGTC 2 cut(s) 108, 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.