Prupe.6G219300_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0014305
Physical Location & Seq
Forward (+)
22552045 .. 22555512
3468 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G219300.1

Sequence Viewer

Length: 549 bp
ATGGGTAATTGCCAAGCGGCGGAGGCGGCGACGGTGCTGATTCAGCACCCGGGAAGCAACAAGGTGGAGAGAATTTACTGGTCTGTGAGCGCCCATGAGGTCATGACTTCCAACCCAGGCCACTACGTGGCTCTTCTCATCGCCTCTCCCACCATGAAGTCTGAGAATGGGGCCCCTGTGAAGCAGCTCAAGCTTCTCAGACCAGACGACACTCTGCTTATTGGACAGGTTTATCGCCTCATCAGTTTCGAAGATGTGTTGAAGGAGTTTGCTGCTAAAAAATCTGTGAAGCTGGGGAAATTGCTGAAGGATACAGGCGGGCCTGGCCTGGAAATGAGGATGAAAGACTTGGGTGGTCCGAATCCAAATCCAAATTTGAAGTCCGAGAACCACAACTCCATCAAGATGGAGCGTGTGAGAAGCAATGGTGGCAGCAGTAGCACAAGCAGCAGCTACAGAAATGTGGCAAGGAATCACGGAGGTGGAGGTGGTGGTGTTGGGGGGCAATGGAGGCCAGCCTTGCAGAGCATTGCAGAGATTGGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

183

Amino Acids

19.43

Weight (kDa)

9.64

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67620
fragaria_vesca FvH4_1g17910
malus_domestica MD02G1187500.v1.1 MD15G1298400.v1.1
prunus_persica Prupe.6G219300_v2.0.a1
pyrus_communis pycom02g15250
rosa_chinensis RchiOBHm_Chr2g0108341
rosa_laevigata RLG00000017662
rosa_multiflora Rmu_sc0002372.1_g000015
rosa_roxburghii Rroxscaffold_2G00135230
rosa_rugosa Rorug02G0152200
rosa_samantha Rh2AG201100 Rh2BG214000 Rh2CG205400 Rh2DG208500
rosa_wichuraiana Rw2G016010 Rw2G016070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 127
AciI CCGC 4 cut(s) 17, 20, 26, 318
AcsI RAATTY 2 cut(s) 72, 373
AcuI CTGAAG 1 cut(s) 326
AdeI CACNNNGTG 1 cut(s) 127
AfiI CCNNNNNNNGG 2 cut(s) 19, 127
AgsI TTSAA 2 cut(s) 262, 379
AjnI CCWGG 3 cut(s) 115, 322, 327
AleI CACNNNNGTG 1 cut(s) 480
AloI GAACNNNNNNTCC 2 cut(s) 380, 412
AluBI AGCT 4 cut(s) 187, 193, 292, 453
AluI AGCT 4 cut(s) 187, 193, 292, 453
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 5 cut(s) 118, 171, 320, 325, 512
ApaI GGGCCC 1 cut(s) 175
ApeKI GCWGC 5 cut(s) 184, 272, 432, 447, 450
ApoI RAATTY 2 cut(s) 72, 373
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 4 cut(s) 171, 172, 320, 356
AsuC2I CCSGG 2 cut(s) 50, 51
AsuII TTCGAA 1 cut(s) 249
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 175
BbvI GCAGC 5 cut(s) 196, 259, 444, 459, 462
BccI CCATC 2 cut(s) 400, 407
BciT130I CCWGG 3 cut(s) 117, 324, 329
BciVI GTATCC 1 cut(s) 304
BcnI CCSGG 2 cut(s) 50, 51
BfmI CTRYAG 1 cut(s) 454
BfoI RGCGCY 1 cut(s) 93
BfuI GTATCC 1 cut(s) 304
BisI GCNGC 7 cut(s) 18, 27, 185, 273, 433, 448, 451
BlsI GCNGC 7 cut(s) 19, 28, 186, 274, 434, 449, 452
Bme1390I CCNGG 5 cut(s) 50, 51, 117, 324, 329
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 4 cut(s) 171, 172, 320, 356
BmiI GGNNCC 3 cut(s) 172, 173, 174
BmrFI CCNGG 5 cut(s) 50, 51, 117, 324, 329
Bpu14I TTCGAA 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 2 cut(s) 50, 51
BsaAI YACGTR 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 49, 115
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 127
Bse1I ACTGG 1 cut(s) 83
Bse3DI GCAATG 3 cut(s) 430, 512, 528
BseBI CCWGG 3 cut(s) 117, 324, 329
BseDI CCNNGG 2 cut(s) 49, 115
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 19, 127
BseMI GCAATG 3 cut(s) 430, 512, 528
BseMII CTCAG 2 cut(s) 153, 211
BseNI ACTGG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 5 cut(s) 196, 259, 444, 459, 462
BseYI CCCAGC 1 cut(s) 292
BshFI GGCC 5 cut(s) 120, 173, 322, 327, 514
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 19, 127
BsnI GGCC 5 cut(s) 120, 173, 322, 327, 514
BsoBI CYCGRG 1 cut(s) 49
Bsp119I TTCGAA 1 cut(s) 249
Bsp120I GGGCCC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 175
BspACI CCGC 4 cut(s) 17, 20, 26, 318
BspANI GGCC 5 cut(s) 120, 173, 322, 327, 514
BspCNI CTCAG 2 cut(s) 154, 210
BspHI TCATGA 1 cut(s) 102
BspLI GGNNCC 3 cut(s) 172, 173, 174
BspQI GCTCTTC 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 249
BsrDI GCAATG 3 cut(s) 430, 512, 528
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 49, 115
Bst2UI CCWGG 3 cut(s) 117, 324, 329
Bst4CI ACNGT 1 cut(s) 34
Bst6I CTCTTC 1 cut(s) 138
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 320, 516
BstDEI CTNAG 2 cut(s) 162, 197
BstF5I GGATG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstNI CCWGG 3 cut(s) 117, 324, 329
BstSCI CCNGG 5 cut(s) 48, 49, 115, 322, 327
BstSFI CTRYAG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 175
BstV1I GCAGC 5 cut(s) 196, 259, 444, 459, 462
BstXI CCANNNNNNTGG 1 cut(s) 406
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 5 cut(s) 120, 173, 322, 327, 514
BtgZI GCGATG 1 cut(s) 124
BtsCI GGATG 1 cut(s) 345
Cac8I GCNNGC 2 cut(s) 320, 516
CciI TCATGA 1 cut(s) 102
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 4 cut(s) 171, 172, 320, 356
Cfr9I CCCGGG 1 cut(s) 49
CviAII CATG 3 cut(s) 95, 103, 154
DdeI CTNAG 2 cut(s) 162, 197
DraIII CACNNNGTG 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
EciI GGCGGA 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 49
EcoO109I RGGNCCY 2 cut(s) 171, 172
EcoRII CCWGG 3 cut(s) 115, 322, 327
EcoT38I GRGCYC 1 cut(s) 175
FaeI CATG 3 cut(s) 98, 106, 157
FaiI YATR 3 cut(s) 96, 104, 155
FatI CATG 3 cut(s) 94, 102, 153
FauI CCCGC 1 cut(s) 311
Fnu4HI GCNGC 7 cut(s) 18, 27, 185, 273, 433, 448, 451
FokI GGATG 1 cut(s) 352
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 7 cut(s) 18, 27, 185, 273, 433, 448, 451
GlaI GCGC 1 cut(s) 91
GluI GCNGC 7 cut(s) 18, 27, 185, 273, 433, 448, 451
GsaI CCCAGC 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 5 cut(s) 120, 173, 322, 327, 514
HapII CCGG 1 cut(s) 50
HhaI GCGC 1 cut(s) 92
Hin1II CATG 3 cut(s) 98, 106, 157
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 3 cut(s) 40, 361, 472
HpaII CCGG 1 cut(s) 50
Hpy188I TCNGA 4 cut(s) 163, 200, 360, 385
Hpy188III TCNNGA 2 cut(s) 103, 403
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 2 cut(s) 256, 301
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 523, 533
HpyF3I CTNAG 2 cut(s) 162, 197
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 3 cut(s) 98, 106, 157
HspAI GCGC 1 cut(s) 90
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 5 cut(s) 196, 259, 444, 459, 462
MaeII ACGT 1 cut(s) 126
MboII GAAGA 2 cut(s) 125, 263
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 4 cut(s) 7, 72, 299, 373
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 8 cut(s) 16, 91, 154, 248, 330, 473, 479, 504
MslI CAYNNNNRTG 2 cut(s) 404, 480
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 5 cut(s) 50, 51, 117, 324, 329
MvaI CCWGG 3 cut(s) 117, 324, 329
NciI CCSGG 2 cut(s) 50, 51
NlaIII CATG 3 cut(s) 98, 106, 157
NlaIV GGNNCC 3 cut(s) 172, 173, 174
NspV TTCGAA 1 cut(s) 249
OliI CACNNNNGTG 1 cut(s) 480
PagI TCATGA 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 138
PfeI GAWTC 3 cut(s) 40, 361, 472
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 7 cut(s) 19, 28, 186, 274, 434, 449, 452
Ppu21I YACGTR 1 cut(s) 127
Psp6I CCWGG 3 cut(s) 115, 322, 327
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 3 cut(s) 115, 322, 327
PspN4I GGNNCC 3 cut(s) 172, 173, 174
PspOMI GGGCCC 1 cut(s) 171
PspPI GGNCC 4 cut(s) 171, 172, 320, 356
RseI CAYNNNNRTG 2 cut(s) 404, 480
SapI GCTCTTC 1 cut(s) 138
SatI GCNGC 7 cut(s) 18, 27, 185, 273, 433, 448, 451
Sau96I GGNCC 4 cut(s) 171, 172, 320, 356
ScrFI CCNGG 5 cut(s) 50, 51, 117, 324, 329
SduI GDGCHC 1 cut(s) 175
SfcI CTRYAG 1 cut(s) 454
SfuI TTCGAA 1 cut(s) 249
SinI GGWCC 1 cut(s) 356
SmaI CCCGGG 1 cut(s) 51
SmiMI CAYNNNNRTG 2 cut(s) 404, 480
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 4 cut(s) 7, 72, 299, 373
SsiI CCGC 4 cut(s) 17, 20, 26, 318
StyD4I CCNGG 5 cut(s) 48, 49, 115, 322, 327
TaaI ACNGT 1 cut(s) 34
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 249
TasI AATT 4 cut(s) 7, 72, 299, 373
TauI GCSGC 2 cut(s) 20, 29
TfiI GAWTC 3 cut(s) 40, 361, 472
TseI GCWGC 5 cut(s) 184, 272, 432, 447, 450
TspDTI ATGAA 2 cut(s) 170, 356
TspGWI ACGGA 1 cut(s) 492
TspMI CCCGGG 1 cut(s) 49
Van91I CCANNNNNTGG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 2 cut(s) 72, 373
XmaI CCCGGG 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.