RchiOBHm_Chr2g0108341

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014305
Physical Location & Seq
Reverse (-)
19757430 .. 19761073
3644 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48227

Sequence Viewer

Length: 630 bp
ATGGGCAACTGTCAGGCGGCGGAGGCGGCGACGGTGATTATTCACCACCCGGCCAACAACAAGGTCGAGAGGATTTACTGGTCGGTGAGCGCTCACGAGATCATGACGTCGAATCCGGGGAACTACGTGGCGCTTGTGGTGACGTCGCCGACGTTGAAGGCCGAGAATGGCGCGCCGGTTAAGCAGCTCAAGCTTCTCAGGCCGGACGACACTTTGCTTATCGGACAGGTTTACCGGCTCATCAGTTTCGAAGATGTGTTGAAGGAGTTTGCTGCTAAAAAGTCTGTGAAGCTGGGGCGGTTGCTGAGGGATAGCGGTGGTCTTGGCCTGGAACTGAAGAAAAAGACTTGGATGGTCCGAATCAGAATTCGAAGTCCGACGACCGCCATTCTACCAAGATGGAGCATGAGGCTCACCGTGTCGGAAGCAGCAGTAGTGGTGGTTGTGGTGGTGGCAGCAGCAGAGGTATGGCGAGGAATCATGGTGGTGGGGGGCAGTGGAGGCCAGCTTTGCAGAGCATTGCAGAACTTGGGACTTGAACATATGTACATGGTGCACTTCCAGTACTCGGGCACACCAGTCACCATTCCCACCACTCACAACAAAGCTCAGAGTCTCAGAGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

209

Amino Acids

22.95

Weight (kDa)

9.86

Isoelectric Point (pI)

32.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 97 9.1e-19 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67620
fragaria_vesca FvH4_1g17910
malus_domestica MD02G1187500.v1.1 MD15G1298400.v1.1
prunus_persica Prupe.6G219300_v2.0.a1
pyrus_communis pycom02g15250
rosa_chinensis RchiOBHm_Chr2g0108341
rosa_laevigata RLG00000017662
rosa_multiflora Rmu_sc0002372.1_g000015
rosa_roxburghii Rroxscaffold_2G00135230
rosa_rugosa Rorug02G0152200
rosa_samantha Rh2AG201100 Rh2BG214000 Rh2CG205400 Rh2DG208500
rosa_wichuraiana Rw2G016010 Rw2G016070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 110, 146
AccII CGCG 1 cut(s) 173
AciI CCGC 6 cut(s) 17, 20, 26, 298, 315, 384
AclWI GGATC 1 cut(s) 617
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 1 cut(s) 366
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 2 cut(s) 107, 143
AfaI GTAC 2 cut(s) 548, 566
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 568
AgsI TTSAA 3 cut(s) 157, 262, 539
AjnI CCWGG 1 cut(s) 327
AluBI AGCT 5 cut(s) 187, 193, 292, 508, 608
AluI AGCT 5 cut(s) 187, 193, 292, 508, 608
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 1 cut(s) 620
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 568
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 5 cut(s) 51, 159, 200, 325, 502
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 5 cut(s) 184, 272, 428, 455, 458
ApoI RAATTY 1 cut(s) 366
AscI GGCGCGCC 1 cut(s) 171
AspLEI GCGC 4 cut(s) 92, 133, 173, 175
AspS9I GGNCC 1 cut(s) 355
AsuC2I CCSGG 2 cut(s) 50, 117
AsuHPI GGTGA 6 cut(s) 35, 46, 97, 151, 406, 574
AsuII TTCGAA 2 cut(s) 249, 370
AvaI CYCGRG 1 cut(s) 568
AvaII GGWCC 1 cut(s) 355
BaeGI GKGCMC 2 cut(s) 558, 575
BauI CACGAG 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 558
BbvCI CCTCAGC 1 cut(s) 305
BbvI GCAGC 5 cut(s) 196, 259, 440, 467, 470
BccI CCATC 2 cut(s) 346, 393
BciT130I CCWGG 1 cut(s) 329
BcnI CCSGG 2 cut(s) 50, 117
BcoDI GTCTC 1 cut(s) 620
BfaI CTAG 1 cut(s) 628
BfoI RGCGCY 2 cut(s) 93, 134
BisI GCNGC 7 cut(s) 18, 27, 185, 273, 429, 456, 459
BlsI GCNGC 7 cut(s) 19, 28, 186, 274, 430, 457, 460
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 3 cut(s) 50, 117, 329
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 355
BmrFI CCNGG 3 cut(s) 50, 117, 329
Bpu10I CCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 2 cut(s) 249, 370
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 2 cut(s) 50, 117
BsaAI YACGTR 1 cut(s) 127
BsaHI GRCGYC 2 cut(s) 107, 143
BsaJI CCNNGG 1 cut(s) 116
Bsc4I CCNNNNNNNGG 1 cut(s) 568
Bse118I RCCGGY 2 cut(s) 175, 234
Bse1I ACTGG 3 cut(s) 83, 562, 578
Bse3DI GCAATG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 1 cut(s) 357
BseLI CCNNNNNNNGG 1 cut(s) 568
BseMI GCAATG 1 cut(s) 518
BseMII CTCAG 3 cut(s) 211, 296, 623
BseNI ACTGG 3 cut(s) 83, 562, 578
BsePI GCGCGC 1 cut(s) 171
BseSI GKGCMC 2 cut(s) 558, 575
BseXI GCAGC 5 cut(s) 196, 259, 440, 467, 470
BseYI CCCAGC 1 cut(s) 292
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 384
BshFI GGCC 5 cut(s) 53, 161, 202, 327, 504
BsiEI CGRYCG 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 558
BsiHKCI CYCGRG 1 cut(s) 568
BsiSI CCGG 5 cut(s) 50, 116, 176, 203, 235
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 1 cut(s) 568
BsmAI GTCTC 1 cut(s) 620
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 5 cut(s) 53, 161, 202, 327, 504
BsoBI CYCGRG 1 cut(s) 568
Bsp119I TTCGAA 2 cut(s) 249, 370
Bsp1286I GDGCHC 2 cut(s) 558, 575
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 2 cut(s) 99, 622
BspACI CCGC 6 cut(s) 17, 20, 26, 298, 315, 384
BspANI GGCC 5 cut(s) 53, 161, 202, 327, 504
BspCNI CTCAG 4 cut(s) 210, 297, 622, 630
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 102
BspPI GGATC 1 cut(s) 617
BspT104I TTCGAA 2 cut(s) 249, 370
BsrDI GCAATG 1 cut(s) 518
BsrFI RCCGGY 2 cut(s) 175, 234
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 3 cut(s) 83, 562, 578
BssAI RCCGGY 2 cut(s) 175, 234
BssECI CCNNGG 1 cut(s) 116
BssHII GCGCGC 1 cut(s) 171
BssMI GATC 2 cut(s) 99, 622
BssNI GRCGYC 2 cut(s) 107, 143
BssSI CACGAG 1 cut(s) 95
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 3 cut(s) 11, 34, 418
BstACI GRCGYC 2 cut(s) 107, 143
BstAUI TGTACA 1 cut(s) 546
BstBAI YACGTR 1 cut(s) 127
BstBI TTCGAA 2 cut(s) 249, 370
BstC8I GCNNGC 2 cut(s) 173, 506
BstDEI CTNAG 4 cut(s) 197, 305, 609, 617
BstF5I GGATG 1 cut(s) 357
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 2 cut(s) 93, 134
BstHHI GCGC 4 cut(s) 92, 133, 173, 175
BstKTI GATC 2 cut(s) 102, 625
BstMAI GTCTC 1 cut(s) 620
BstMBI GATC 2 cut(s) 99, 622
BstMCI CGRYCG 1 cut(s) 384
BstMWI GCNNNNNNNGC 7 cut(s) 23, 26, 181, 190, 199, 501, 510
BstNI CCWGG 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 48, 115, 327
BstSLI GKGCMC 2 cut(s) 558, 575
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 5 cut(s) 196, 259, 440, 467, 470
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
BsuRI GGCC 5 cut(s) 53, 161, 202, 327, 504
BtsCI GGATG 1 cut(s) 357
BtsI GCAGTG 1 cut(s) 502
BtsIMutI CAGTG 1 cut(s) 502
Cac8I GCNNGC 2 cut(s) 173, 506
CciI TCATGA 1 cut(s) 102
CfoI GCGC 4 cut(s) 92, 133, 173, 175
Cfr10I RCCGGY 2 cut(s) 175, 234
Cfr13I GGNCC 1 cut(s) 355
Csp6I GTAC 2 cut(s) 547, 565
CviAII CATG 4 cut(s) 103, 406, 481, 550
CviQI GTAC 2 cut(s) 547, 565
DdeI CTNAG 4 cut(s) 197, 305, 609, 617
DpnI GATC 2 cut(s) 101, 624
DpnII GATC 2 cut(s) 99, 622
EaeI YGGCCR 1 cut(s) 51
EciI GGCGGA 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 355
Eco47III AGCGCT 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 568
EcoRI GAATTC 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 327
FaeI CATG 4 cut(s) 106, 409, 484, 553
FaiI YATR 7 cut(s) 104, 407, 469, 482, 543, 545, 551
FaqI GGGAC 1 cut(s) 546
FatI CATG 4 cut(s) 102, 405, 480, 549
FauNDI CATATG 1 cut(s) 543
Fnu4HI GCNGC 7 cut(s) 18, 27, 185, 273, 429, 456, 459
FokI GGATG 1 cut(s) 364
Fsp4HI GCNGC 7 cut(s) 18, 27, 185, 273, 429, 456, 459
FspBI CTAG 1 cut(s) 628
GlaI GCGC 4 cut(s) 91, 132, 172, 174
GluI GCNGC 7 cut(s) 18, 27, 185, 273, 429, 456, 459
GsaI CCCAGC 1 cut(s) 296
HaeII RGCGCY 2 cut(s) 93, 134
HaeIII GGCC 5 cut(s) 53, 161, 202, 327, 504
HapII CCGG 5 cut(s) 50, 116, 176, 203, 235
HhaI GCGC 4 cut(s) 92, 133, 173, 175
Hin1I GRCGYC 2 cut(s) 107, 143
Hin1II CATG 4 cut(s) 106, 409, 484, 553
Hin6I GCGC 4 cut(s) 90, 131, 171, 173
HinP1I GCGC 4 cut(s) 90, 131, 171, 173
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 4 cut(s) 112, 360, 477, 613
HpaII CCGG 5 cut(s) 50, 116, 176, 203, 235
HphI GGTGA 6 cut(s) 35, 46, 97, 151, 406, 574
Hpy166II GTNNAC 2 cut(s) 232, 556
Hpy188I TCNGA 7 cut(s) 224, 359, 365, 378, 424, 612, 620
Hpy188III TCNNGA 3 cut(s) 67, 95, 103
Hpy8I GTNNAC 2 cut(s) 232, 556
Hpy99I CGWCG 5 cut(s) 34, 112, 148, 154, 382
HpyAV CCTTC 2 cut(s) 151, 256
HpyCH4III ACNGT 3 cut(s) 11, 34, 418
HpyCH4IV ACGT 4 cut(s) 107, 126, 143, 152
HpyCH4V TGCA 3 cut(s) 513, 523, 556
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 26, 181, 190, 199, 501, 510
HpyF3I CTNAG 4 cut(s) 197, 305, 609, 617
HpySE526I ACGT 4 cut(s) 107, 126, 143, 152
Hsp92I GRCGYC 2 cut(s) 107, 143
Hsp92II CATG 4 cut(s) 106, 409, 484, 553
HspAI GCGC 4 cut(s) 90, 131, 171, 173
Kzo9I GATC 2 cut(s) 99, 622
LmnI GCTCC 1 cut(s) 402
Lsp1109I GCAGC 5 cut(s) 196, 259, 440, 467, 470
MaeI CTAG 1 cut(s) 628
MaeII ACGT 4 cut(s) 107, 126, 143, 152
MaeIII GTNAC 2 cut(s) 139, 580
MalI GATC 2 cut(s) 101, 624
MboI GATC 2 cut(s) 99, 622
MboII GAAGA 2 cut(s) 263, 349
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 2 cut(s) 558, 575
MluCI AATT 1 cut(s) 366
MlyI GAGTC 1 cut(s) 622
MmeI TCCRAC 2 cut(s) 401, 402
MnlI CCTC 7 cut(s) 16, 63, 300, 402, 457, 467, 494
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 1 cut(s) 485
MspI CCGG 5 cut(s) 50, 116, 176, 203, 235
MspR9I CCNGG 3 cut(s) 50, 117, 329
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 7 cut(s) 23, 26, 181, 190, 199, 501, 510
NciI CCSGG 2 cut(s) 50, 117
NdeI CATATG 1 cut(s) 543
NdeII GATC 2 cut(s) 99, 622
NlaIII CATG 4 cut(s) 106, 409, 484, 553
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 2 cut(s) 139, 580
NspV TTCGAA 2 cut(s) 249, 370
PagI TCATGA 1 cut(s) 102
PalAI GGCGCGCC 1 cut(s) 171
PauI GCGCGC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PfeI GAWTC 3 cut(s) 112, 360, 477
PkrI GCNGC 7 cut(s) 19, 28, 186, 274, 430, 457, 460
PleI GAGTC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 621
Ppu21I YACGTR 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 327
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 1 cut(s) 327
PspPI GGNCC 1 cut(s) 355
PsuI RGATCY 1 cut(s) 622
PteI GCGCGC 1 cut(s) 171
RsaI GTAC 2 cut(s) 548, 566
RsaNI GTAC 2 cut(s) 547, 565
RseI CAYNNNNRTG 1 cut(s) 485
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 7 cut(s) 18, 27, 185, 273, 429, 456, 459
Sau3AI GATC 2 cut(s) 99, 622
Sau96I GGNCC 1 cut(s) 355
ScaI AGTACT 1 cut(s) 566
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 3 cut(s) 50, 117, 329
SduI GDGCHC 2 cut(s) 558, 575
SfuI TTCGAA 2 cut(s) 249, 370
SgsI GGCGCGCC 1 cut(s) 171
SinI GGWCC 1 cut(s) 355
SmiMI CAYNNNNRTG 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 1 cut(s) 366
SsiI CCGC 6 cut(s) 17, 20, 26, 298, 315, 384
SspMI CTAG 1 cut(s) 628
StyD4I CCNGG 3 cut(s) 48, 115, 327
TaaI ACNGT 3 cut(s) 11, 34, 418
TaiI ACGT 4 cut(s) 110, 129, 146, 155
TaqI TCGA 4 cut(s) 66, 110, 249, 370
TasI AATT 1 cut(s) 366
TatI WGTACW 2 cut(s) 546, 564
TauI GCSGC 2 cut(s) 20, 29
TfiI GAWTC 3 cut(s) 112, 360, 477
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 2 cut(s) 139, 580
TseI GCWGC 5 cut(s) 184, 272, 428, 455, 458
Tsp45I GTSAC 2 cut(s) 139, 580
TspRI CASTG 1 cut(s) 502
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 1 cut(s) 355
XapI RAATTY 1 cut(s) 366
XspI CTAG 1 cut(s) 628
ZraI GACGTC 2 cut(s) 108, 144
ZrmI AGTACT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.