MD15G1298400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014305
Physical Location & Seq
Reverse (-)
28132459 .. 28136494
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1298400.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGGAAATTGTCAGGCGGCGGAGGCAGCGACGGTGGTGATTCAGCACCCCGGGGCCAACAAGATCGAGAGGATTTACTGGTCTGTGGGCGCTCATGAGGTCATGACTTCCAACCCTGGTCACTACGTGGCTCTTGTCGTCAACTCTCCCACCATGAAGTCTGAGACCGGTGCTCCAGTGAAGCAGCTCAAGCTTCTCAGACCAGACGACACTTTACTCATTGGACAGGTTTATCGCCTCATCAGTTTTGAAGATGTGTTGAAGGAGTTTGCTGCCAAAAAGTCCGTGAAGCTGGGAAAGTTGCTGAAGGATAGAGGTGGGCTTGGAGTGGAAATGAAGATGAAGGACCTGGCGGTTCAGAATTTGAAGTCCGGGAACAACAATTCTGTCAAGATGGAGAGTGTGGGAAGCAGCTGTGGCAGCAAAAGCACTAACAACGGCAGAAGCTACAGAAGTGTGGGAAGGAACCATGGTGGTGTAGGTGGTGGGGAGCAGTGGAGGCCTGCCTTGCAGAGCATTGCAGAGGTTGGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

179

Amino Acids

19.04

Weight (kDa)

9.6

Isoelectric Point (pI)

29.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 175 4.7e-26 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67620
fragaria_vesca FvH4_1g17910
malus_domestica MD02G1187500.v1.1 MD15G1298400.v1.1
prunus_persica Prupe.6G219300_v2.0.a1
pyrus_communis pycom02g15250
rosa_chinensis RchiOBHm_Chr2g0108341
rosa_laevigata RLG00000017662
rosa_multiflora Rmu_sc0002372.1_g000015
rosa_roxburghii Rroxscaffold_2G00135230
rosa_rugosa Rorug02G0152200
rosa_samantha Rh2AG201100 Rh2BG214000 Rh2CG205400 Rh2DG208500
rosa_wichuraiana Rw2G016010 Rw2G016070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 17, 20, 353
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 326
AdeI CACNNNGTG 1 cut(s) 127
AgeI ACCGGT 1 cut(s) 167
AgsI TTSAA 3 cut(s) 251, 262, 367
AjnI CCWGG 2 cut(s) 115, 348
AluBI AGCT 5 cut(s) 187, 193, 292, 414, 447
AluI AGCT 5 cut(s) 187, 193, 292, 414, 447
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 2 cut(s) 54, 500
ApeKI GCWGC 5 cut(s) 26, 184, 272, 411, 420
ApoI RAATTY 1 cut(s) 361
AsiGI ACCGGT 1 cut(s) 167
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 54, 346
AsuC2I CCSGG 3 cut(s) 51, 52, 373
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 346
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 5 cut(s) 38, 196, 259, 423, 432
BccI CCATC 1 cut(s) 388
BceAI ACGGC 1 cut(s) 454
BciT130I CCWGG 2 cut(s) 117, 350
BcnI CCSGG 3 cut(s) 51, 52, 373
BcoDI GTCTC 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 448
BfoI RGCGCY 1 cut(s) 93
BisI GCNGC 6 cut(s) 18, 27, 185, 273, 412, 421
BlsI GCNGC 6 cut(s) 19, 28, 186, 274, 413, 422
Bme1390I CCNGG 5 cut(s) 51, 52, 117, 350, 373
Bme18I GGWCC 1 cut(s) 346
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 54, 346
BmiI GGNNCC 2 cut(s) 55, 467
BmrFI CCNGG 5 cut(s) 51, 52, 117, 350, 373
BpmI CTGGAG 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 3 cut(s) 51, 52, 373
BsaAI YACGTR 1 cut(s) 127
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 5 cut(s) 49, 50, 51, 115, 469
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 83, 176
Bse3DI GCAATG 1 cut(s) 516
BseBI CCWGG 2 cut(s) 117, 350
BseDI CCNNGG 5 cut(s) 49, 50, 51, 115, 469
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 2 cut(s) 153, 211
BseNI ACTGG 2 cut(s) 83, 176
BseXI GCAGC 5 cut(s) 38, 196, 259, 423, 432
BseYI CCCAGC 1 cut(s) 292
BshFI GGCC 2 cut(s) 56, 502
BshTI ACCGGT 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 51, 168, 372
BsmAI GTCTC 1 cut(s) 158
BsnI GGCC 2 cut(s) 56, 502
Bso31I GGTCTC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 1 cut(s) 63
Bsp19I CCATGG 1 cut(s) 469
BspACI CCGC 3 cut(s) 17, 20, 353
BspANI GGCC 2 cut(s) 56, 502
BspCNI CTCAG 2 cut(s) 154, 210
BspHI TCATGA 2 cut(s) 94, 102
BspLI GGNNCC 2 cut(s) 55, 467
BspTNI GGTCTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 516
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 2 cut(s) 83, 176
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 5 cut(s) 49, 50, 51, 115, 469
BssMI GATC 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 469
Bst2UI CCWGG 2 cut(s) 117, 350
Bst4CI ACNGT 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 504
BstDEI CTNAG 2 cut(s) 162, 197
BstDSI CCRYGG 1 cut(s) 469
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 8 cut(s) 23, 26, 190, 417, 420, 426, 499, 508
BstNI CCWGG 2 cut(s) 117, 350
BstSCI CCNGG 5 cut(s) 49, 50, 115, 348, 371
BstSFI CTRYAG 1 cut(s) 448
BstV1I GCAGC 5 cut(s) 38, 196, 259, 423, 432
BsuRI GGCC 2 cut(s) 56, 502
BtgI CCRYGG 1 cut(s) 469
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 2 cut(s) 183, 500
Cac8I GCNNGC 1 cut(s) 504
CciI TCATGA 2 cut(s) 94, 102
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 54, 346
Cfr9I CCCGGG 1 cut(s) 50
CspAI ACCGGT 1 cut(s) 167
CviAII CATG 4 cut(s) 95, 103, 154, 470
CviJI RGCY 9 cut(s) 56, 131, 187, 193, 292, 322, 414, 447, 502
CviKI_1 RGCY 9 cut(s) 56, 131, 187, 193, 292, 322, 414, 447, 502
DdeI CTNAG 2 cut(s) 162, 197
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
DraIII CACNNNGTG 1 cut(s) 127
EciI GGCGGA 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 469
Eco147I AGGCCT 1 cut(s) 502
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 115, 348
EcoT14I CCWWGG 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 469
FaeI CATG 4 cut(s) 98, 106, 157, 473
FaiI YATR 4 cut(s) 96, 104, 155, 471
FatI CATG 4 cut(s) 94, 102, 153, 469
Fnu4HI GCNGC 6 cut(s) 18, 27, 185, 273, 412, 421
Fsp4HI GCNGC 6 cut(s) 18, 27, 185, 273, 412, 421
GlaI GCGC 1 cut(s) 91
GluI GCNGC 6 cut(s) 18, 27, 185, 273, 412, 421
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 2 cut(s) 56, 502
HapII CCGG 3 cut(s) 51, 168, 372
HhaI GCGC 1 cut(s) 92
Hin1II CATG 4 cut(s) 98, 106, 157, 473
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 1 cut(s) 40
HpaII CCGG 3 cut(s) 51, 168, 372
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 3 cut(s) 163, 200, 360
Hpy188III TCNNGA 4 cut(s) 67, 95, 103, 391
Hpy8I GTNNAC 1 cut(s) 142
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 4 cut(s) 256, 301, 337, 456
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 511, 521
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 26, 190, 417, 420, 426, 499, 508
HpyF3I CTNAG 2 cut(s) 162, 197
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 4 cut(s) 98, 106, 157, 473
HspAI GCGC 1 cut(s) 90
Kzo9I GATC 1 cut(s) 63
LmnI GCTCC 2 cut(s) 178, 490
Lsp1109I GCAGC 5 cut(s) 38, 196, 259, 423, 432
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 119
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 2 cut(s) 263, 349
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 3 cut(s) 7, 361, 382
MmeI TCCRAC 2 cut(s) 135, 508
MnlI CCTC 7 cut(s) 16, 63, 91, 248, 308, 492, 517
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 414
MspI CCGG 3 cut(s) 51, 168, 372
MspR9I CCNGG 5 cut(s) 51, 52, 117, 350, 373
MvaI CCWGG 2 cut(s) 117, 350
MwoI GCNNNNNNNGC 8 cut(s) 23, 26, 190, 417, 420, 426, 499, 508
NciI CCSGG 3 cut(s) 51, 52, 373
NcoI CCATGG 1 cut(s) 469
NdeII GATC 1 cut(s) 63
NlaIII CATG 4 cut(s) 98, 106, 157, 473
NlaIV GGNNCC 2 cut(s) 55, 467
NmuCI GTSAC 1 cut(s) 119
PagI TCATGA 2 cut(s) 94, 102
PceI AGGCCT 1 cut(s) 502
PfeI GAWTC 1 cut(s) 40
PfoI TCCNGGA 1 cut(s) 371
PinAI ACCGGT 1 cut(s) 167
PkrI GCNGC 6 cut(s) 19, 28, 186, 274, 413, 422
Ppu21I YACGTR 1 cut(s) 127
PpuMI RGGWCCY 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 115, 348
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 2 cut(s) 115, 348
PspN4I GGNNCC 2 cut(s) 55, 467
PspPI GGNCC 2 cut(s) 54, 346
PspPPI RGGWCCY 1 cut(s) 346
PvuII CAGCTG 1 cut(s) 414
RseI CAYNNNNRTG 1 cut(s) 474
SatI GCNGC 6 cut(s) 18, 27, 185, 273, 412, 421
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 2 cut(s) 54, 346
ScrFI CCNGG 5 cut(s) 51, 52, 117, 350, 373
SduI GDGCHC 1 cut(s) 175
SfcI CTRYAG 1 cut(s) 448
SinI GGWCC 1 cut(s) 346
SmaI CCCGGG 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 3 cut(s) 7, 361, 382
SseBI AGGCCT 1 cut(s) 502
SsiI CCGC 3 cut(s) 17, 20, 353
StuI AGGCCT 1 cut(s) 502
StyD4I CCNGG 5 cut(s) 49, 50, 115, 348, 371
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 1 cut(s) 34
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 66
TasI AATT 3 cut(s) 7, 361, 382
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 1 cut(s) 40
TscAI CASTG 2 cut(s) 183, 500
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 5 cut(s) 26, 184, 272, 411, 420
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 3 cut(s) 170, 350, 356
TspGWI ACGGA 1 cut(s) 274
TspMI CCCGGG 1 cut(s) 50
TspRI CASTG 2 cut(s) 183, 500
VpaK11BI GGWCC 1 cut(s) 346
XapI RAATTY 1 cut(s) 361
XmaI CCCGGG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.