ATMG00280

RuBisCO catalyzes two reactions the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Mt
OG0018283
Physical Location & Seq
Reverse (-)
77819 .. 78151
333 bp
Loading structure...
UTR
Exon/CDS
Intron
ATMG00280.1

Sequence Viewer

Length: 333 bp
ATGAACAATGCGGCTAAGCGTGCAGATTGTTGGTTTGGCGCGAAGAACTATGGTAGAGCAGTTTATGAATGTCTACGTGGTGGACTTTATTTTACCAAAGATGATGAGAATGTGAACTCCCAACCATTTATGCGTTGGAGAGACCGTTTCTTATTTTGTGCCGAAGCTGTTTATAAAGCACAGGCTGAAACAGGTGGAATCAAAGGGCATTATTTGAATGCTACTGCGGGTACATGCGAAGAAATGATCAAAAGAGCTGTATTTGCCAGAGAATTGGGAGTTCCTATCGTAATGCATGACTACTTAAACAGGGGGATTCACCGCAAATACTAG

Protein Analysis

110

Amino Acids

12.66

Weight (kDa)

9.14

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCO_large PF00016 14 - 106 5.4e-37 Ribulose bisphosphate carboxylase large chain, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07732 ATMG00280
malus_domestica MD07G1039000.v1.1 MD07G1110300.v1.1
rosa_chinensis RchiOBHm_Chr5g0043001
rosa_laevigata RLG00000030425
rosa_multiflora Rmu_sc0000239.1_g000052
rosa_samantha Rh5BG294900 Rh5DG304700
rosa_wichuraiana Rw5G025270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
AccI GTMKAC 1 cut(s) 73
AccII CGCG 1 cut(s) 41
AciI CCGC 3 cut(s) 11, 227, 322
AfaI GTAC 1 cut(s) 232
AgsI TTSAA 1 cut(s) 217
AluBI AGCT 2 cut(s) 167, 257
AluI AGCT 2 cut(s) 167, 257
Alw26I GTCTC 1 cut(s) 135
AspLEI GCGC 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 311
BclI TGATCA 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 1 cut(s) 331
BisI GCNGC 1 cut(s) 12
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 13
Bpu1102I GCTNAGC 1 cut(s) 15
BsaAI YACGTR 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 135
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
BsgI GTGCAG 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 135
BsmI GAATGC 1 cut(s) 223
Bso31I GGTCTC 1 cut(s) 135
Bsp143I GATC 1 cut(s) 246
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 3 cut(s) 11, 227, 322
BspFNI CGCG 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 135
BssMI GATC 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 1 cut(s) 15
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 2 cut(s) 20, 263
BstNSI RCATGY 1 cut(s) 237
BstUI CGCG 1 cut(s) 41
BstXI CCANNNNNNTGG 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 21
CfoI GCGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 231
CviAII CATG 2 cut(s) 234, 296
CviJI RGCY 4 cut(s) 14, 167, 185, 257
CviKI_1 RGCY 4 cut(s) 14, 167, 185, 257
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 1 cut(s) 15
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 135
EcoT22I ATGCAT 1 cut(s) 297
FaeI CATG 2 cut(s) 237, 299
FaiI YATR 6 cut(s) 51, 66, 131, 174, 235, 297
FatI CATG 2 cut(s) 233, 295
FauI CCCGC 1 cut(s) 220
FbaI TGATCA 1 cut(s) 246
FblI GTMKAC 1 cut(s) 73
Fnu4HI GCNGC 1 cut(s) 12
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 331
GlaI GCGC 1 cut(s) 40
GluI GCNGC 1 cut(s) 12
HhaI GCGC 1 cut(s) 41
Hin1II CATG 2 cut(s) 237, 299
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HinfI GANTC 2 cut(s) 198, 316
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 3 cut(s) 74, 83, 115
Hpy8I GTNNAC 3 cut(s) 74, 83, 115
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 23, 295
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 263
HpyF3I CTNAG 1 cut(s) 15
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 2 cut(s) 237, 299
HspAI GCGC 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 1 cut(s) 246
LpnPI CCDG 4 cut(s) 167, 177, 280, 295
MaeI CTAG 1 cut(s) 331
MaeII ACGT 1 cut(s) 76
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 2 cut(s) 55, 251
MluCI AATT 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 116
Mph1103I ATGCAT 1 cut(s) 297
MseI TTAA 1 cut(s) 305
Mva1269I GAATGC 1 cut(s) 223
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 20, 263
NdeII GATC 1 cut(s) 246
NlaIII CATG 2 cut(s) 237, 299
NsiI ATGCAT 1 cut(s) 297
NspI RCATGY 1 cut(s) 237
PctI GAATGC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 198, 316
PkrI GCNGC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 77
PsiI TTATAA 1 cut(s) 174
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 1 cut(s) 246
SetI ASST 4 cut(s) 79, 169, 196, 259
Sse9I AATT 1 cut(s) 272
SsiI CCGC 3 cut(s) 11, 227, 322
SspMI CTAG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 1 cut(s) 79
TasI AATT 1 cut(s) 272
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 2 cut(s) 198, 316
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TspDTI ATGAA 2 cut(s) 17, 81
XceI RCATGY 1 cut(s) 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 132
XmiI GTMKAC 1 cut(s) 73
XspI CTAG 1 cut(s) 331
Zsp2I ATGCAT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.