Rh5BG294900

Ribulose bisphosphate carboxylase large chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018283
Physical Location & Seq
Forward (+)
37611574 .. 37611873
300 bp
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UTR
Exon/CDS
Intron
Rh5BG294900.1

Sequence Viewer

Length: 300 bp
ATGATGAAAAGAGCCGTATTTGCCAGAGAATTGGGAGTTCCTATCGTAATGCACGATTACTTAACGGGGGGATTCACTGCAAATACTACCTTGGCTCATTATTGCCGAGATAATGGTCTACTTCTTCACATCCACCGTGCAATGCATGCTGTTATTGATAGACAGAAGAATCATGGTATGCACTTTCGTGTACTAGCTAAAGCATTACGTATGTCTGGTGGAGATCATATCCACGCTGGTACCGTAGTAGGTAAACTTGAGGGGGAAAGAGAAATCACTTTAGGCTTATTTTACTCATGA

Protein Analysis

99

Amino Acids

11.08

Weight (kDa)

9.65

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCO_large PF00016 1 - 95 3.9e-37 Ribulose bisphosphate carboxylase large chain, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07732 ATMG00280
malus_domestica MD07G1039000.v1.1 MD07G1110300.v1.1
rosa_chinensis RchiOBHm_Chr5g0043001
rosa_laevigata RLG00000030425
rosa_multiflora Rmu_sc0000239.1_g000052
rosa_samantha Rh5BG294900 Rh5DG304700
rosa_wichuraiana Rw5G025270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 118
AfaI GTAC 2 cut(s) 192, 241
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 1 cut(s) 197
AluI AGCT 1 cut(s) 197
Asp718I GGTACC 1 cut(s) 239
BaeI ACNNNNGTAYC 4 cut(s) 223, 231, 256, 264
BanI GGYRCC 1 cut(s) 239
BfaI CTAG 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 90
Bse3DI GCAATG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 129
BseMI GCAATG 1 cut(s) 147
BshNI GGYRCC 1 cut(s) 239
Bsp143I GATC 1 cut(s) 223
BspHI TCATGA 1 cut(s) 296
BspLI GGNNCC 1 cut(s) 241
BspT107I GGYRCC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 147
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 1 cut(s) 223
BssT1I CCWWGG 1 cut(s) 90
Bst4CI ACNGT 2 cut(s) 137, 244
BstAPI GCANNNNNTGC 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 147
BstF5I GGATG 1 cut(s) 129
BstKTI GATC 1 cut(s) 226
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 2 cut(s) 20, 146
BstNSI RCATGY 1 cut(s) 149
BstSNI TACGTA 1 cut(s) 209
BstXI CCANNNNNNTGG 1 cut(s) 31
BtsCI GGATG 1 cut(s) 129
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 147
CciI TCATGA 1 cut(s) 296
Csp6I GTAC 2 cut(s) 191, 240
CviAII CATG 3 cut(s) 146, 173, 297
CviJI RGCY 4 cut(s) 14, 95, 197, 285
CviKI_1 RGCY 4 cut(s) 14, 95, 197, 285
CviQI GTAC 2 cut(s) 191, 240
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
Eco105I TACGTA 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 3 cut(s) 149, 176, 300
FaiI YATR 6 cut(s) 147, 174, 179, 212, 228, 298
FatI CATG 3 cut(s) 145, 172, 296
FblI GTMKAC 1 cut(s) 118
FokI GGATG 1 cut(s) 116
FspBI CTAG 1 cut(s) 194
Hin1II CATG 3 cut(s) 149, 176, 300
HinfI GANTC 2 cut(s) 72, 169
Hpy166II GTNNAC 3 cut(s) 119, 191, 254
Hpy188III TCNNGA 1 cut(s) 297
Hpy8I GTNNAC 3 cut(s) 119, 191, 254
HpyCH4III ACNGT 2 cut(s) 137, 244
HpyCH4IV ACGT 1 cut(s) 208
HpyCH4V TGCA 5 cut(s) 52, 80, 140, 145, 181
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 146
HpySE526I ACGT 1 cut(s) 208
Hsp92II CATG 3 cut(s) 149, 176, 300
KpnI GGTACC 1 cut(s) 243
Kzo9I GATC 1 cut(s) 223
LpnPI CCDG 3 cut(s) 37, 201, 222
MaeI CTAG 1 cut(s) 194
MaeII ACGT 1 cut(s) 208
MalI GATC 1 cut(s) 225
MboI GATC 1 cut(s) 223
MboII GAAGA 2 cut(s) 116, 178
MluCI AATT 1 cut(s) 29
MnlI CCTC 1 cut(s) 253
Mph1103I ATGCAT 1 cut(s) 147
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 20, 146
NdeII GATC 1 cut(s) 223
NlaIII CATG 3 cut(s) 149, 176, 300
NlaIV GGNNCC 1 cut(s) 241
NmeAIII GCCGAG 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 147
NspI RCATGY 1 cut(s) 149
OliI CACNNNNGTG 1 cut(s) 186
PaeI GCATGC 1 cut(s) 149
PagI TCATGA 1 cut(s) 296
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 240
PfeI GAWTC 2 cut(s) 72, 169
Ppu21I YACGTR 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 241
RsaI GTAC 2 cut(s) 192, 241
RsaNI GTAC 2 cut(s) 191, 240
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 1 cut(s) 62
Sau3AI GATC 1 cut(s) 223
SetI ASST 4 cut(s) 92, 199, 211, 253
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SnaBI TACGTA 1 cut(s) 209
SphI GCATGC 1 cut(s) 149
Sse9I AATT 1 cut(s) 29
SspMI CTAG 1 cut(s) 194
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 137, 244
TaiI ACGT 1 cut(s) 211
TasI AATT 1 cut(s) 29
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 2 cut(s) 72, 169
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 1 cut(s) 82
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 1 cut(s) 82
XceI RCATGY 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 118
XspI CTAG 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.