Rw5G025270

Ribulose bisphosphate carboxylase, large chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018283
Physical Location & Seq
Reverse (-)
35932738 .. 35933040
303 bp
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UTR
Exon/CDS
Intron
Rw5G025270.1

Sequence Viewer

Length: 303 bp
ATGCGTTGGAGAGACCGTTTCTTATTTTGTGCCGAAGCAATTTATAAAGCGCAGGCTGAAACAGGTGAAATCAAAGGGCATTACTTGAATGCTACTGCAGGTACATGCGAAGACATGATGAAAAGAGCCGTATTTGCCAGAGAATTGGGAGTTCCTATCGTAATGCACGATTACTTAACGGGGGGATTCACTGCAAATACTACCTTGGCTCATTATTGCCGAGATAATGGTCTACTTCTTCACATCCACCGTGCAATGCATGCTGTTATTGATAGACAGAAGAATCATGGTGAAATCTCTTGA

Protein Analysis

100

Amino Acids

11.38

Weight (kDa)

7.82

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuBisCO_large PF00016 1 - 97 4e-38 Ribulose bisphosphate carboxylase large chain, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07732 ATMG00280
malus_domestica MD07G1039000.v1.1 MD07G1110300.v1.1
rosa_chinensis RchiOBHm_Chr5g0043001
rosa_laevigata RLG00000030425
rosa_multiflora Rmu_sc0000239.1_g000052
rosa_samantha Rh5BG294900 Rh5DG304700
rosa_wichuraiana Rw5G025270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 45
Acc36I ACCTGC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 232
AfaI GTAC 1 cut(s) 103
AgsI TTSAA 1 cut(s) 88
Alw26I GTCTC 1 cut(s) 6
AspLEI GCGC 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 77, 302
BbsI GAAGAC 1 cut(s) 117
BceAI ACGGC 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 96
BfuAI ACCTGC 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 204
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 1 cut(s) 243
BseMI GCAATG 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 6
BsmI GAATGC 1 cut(s) 94
Bso31I GGTCTC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 261
BssECI CCNNGG 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 204
Bst4CI ACNGT 2 cut(s) 17, 251
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 2 cut(s) 54, 261
BstF5I GGATG 1 cut(s) 243
BstHHI GCGC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 6
BstMWI GCNNNNNNNGC 2 cut(s) 134, 260
BstNSI RCATGY 2 cut(s) 108, 263
BstSFI CTRYAG 1 cut(s) 96
BstV2I GAAGAC 1 cut(s) 117
BstXI CCANNNNNNTGG 1 cut(s) 145
BtsCI GGATG 1 cut(s) 243
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 2 cut(s) 54, 261
CfoI GCGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 102
CviAII CATG 4 cut(s) 105, 115, 260, 287
CviJI RGCY 3 cut(s) 56, 128, 209
CviKI_1 RGCY 3 cut(s) 56, 128, 209
CviQI GTAC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 4 cut(s) 108, 118, 263, 290
FaiI YATR 5 cut(s) 45, 106, 116, 261, 288
FatI CATG 4 cut(s) 104, 114, 259, 286
FblI GTMKAC 1 cut(s) 232
FokI GGATG 1 cut(s) 230
GlaI GCGC 1 cut(s) 51
HhaI GCGC 1 cut(s) 52
Hin1II CATG 4 cut(s) 108, 118, 263, 290
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HinfI GANTC 2 cut(s) 186, 283
HphI GGTGA 2 cut(s) 77, 302
Hpy166II GTNNAC 1 cut(s) 233
Hpy188III TCNNGA 1 cut(s) 300
Hpy8I GTNNAC 1 cut(s) 233
HpyCH4III ACNGT 2 cut(s) 17, 251
HpyCH4V TGCA 5 cut(s) 98, 166, 194, 254, 259
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 260
Hsp92II CATG 4 cut(s) 108, 118, 263, 290
HspAI GCGC 1 cut(s) 50
LpnPI CCDG 4 cut(s) 38, 48, 84, 151
MboII GAAGA 3 cut(s) 122, 230, 292
MluCI AATT 2 cut(s) 39, 143
Mph1103I ATGCAT 1 cut(s) 261
MseI TTAA 1 cut(s) 176
Mva1269I GAATGC 1 cut(s) 94
MwoI GCNNNNNNNGC 2 cut(s) 134, 260
NlaIII CATG 4 cut(s) 108, 118, 263, 290
NmeAIII GCCGAG 1 cut(s) 245
NsiI ATGCAT 1 cut(s) 261
NspI RCATGY 2 cut(s) 108, 263
PaeI GCATGC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 94
PfeI GAWTC 2 cut(s) 186, 283
PsiI TTATAA 1 cut(s) 45
PstI CTGCAG 1 cut(s) 100
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 176
SetI ASST 3 cut(s) 67, 103, 206
SfcI CTRYAG 1 cut(s) 96
SphI GCATGC 1 cut(s) 263
Sse9I AATT 2 cut(s) 39, 143
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 2 cut(s) 17, 251
TasI AATT 2 cut(s) 39, 143
TfiI GAWTC 2 cut(s) 186, 283
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 134
TspRI CASTG 1 cut(s) 196
XceI RCATGY 2 cut(s) 108, 263
XmiI GTMKAC 1 cut(s) 232
Zsp2I ATGCAT 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.