Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0005972

Physical Location & Seq

Genomic Coordinates Forward (+)

3204406 .. 3211216

Gene Structure 6811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g06000.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1923 bp

ATGGACACATTAATACCCCCCGGCAAACTGAGCGGCCGTGGACGAACAAGTTCGAGACACAAAGCATTATGGCGGTGCTTGTGGAGAGGTTTTGTGAGAAGGAAGGAAAAACACAGCCACAGAAAGAGCAACTTGACTCTAGTGAGAATGGATAGCACACTTAAGGAGATGAGGGATGGTGTTTCTGCACTTGACCTGGACTCCAAATCCGCCGTCGGTGGCGGCGTTGAGGACATCTACGGTGAGGATGCTGCCACTGAGGACCAACTTGTCACCCCCTGGACTTACTCTGTCGCCAGTGGGTATTCTTTGCTGAGAGATCCTCAATACAACAAAGGGCTTGCATTTACTGAGAAAGAGAGGGATGCTCATTACTTGCGTGGTCTTCTTCCTCCGGCAACTTCCAGTCAAGAACTTCAGGAAAAGAAGCTGATGCACAATCTCAGACAATACCAAGTGCCGCTGCAAAAGTATATGGCACTGACCGAACTCCAGGAGAGGAATGAGAGGCTGTTCTACAAACTTCTCATTGACAATGTCGAGGAATTGCTCCCGATTGTATATACTCCAACTGTTGGTGAGGCTTGCCAGAAATATGGAAGCATCTTCAGACGCCCTCAGGGTCTTTACATAAGTCTGAAAGAGAAAGGTAGAATTCTCGAGGTATTGAAGAACTGGCCTGAAAGGACTATTCAAGTTATTGTTGTAACTGATGGTGAGCGTATTTTGGGACTTGGTGACCTTGGTTGTCAGGGGATGGGAATTCCTGTGGGGAAATTGGCTTTGTATACAGCTCTTGGAGGAGTTCGTCCCTCAACATGTTTGCCTATTACCATTGATGTGGGGACAAATAATGAACAATTGCTGAAGGATGAATTCTACATTGGGCTCAGGCAAAAGAGAGCAACTGGAAAGGAGTATGCTGAGCTTTTACATGAGTTCATGGGCGCTGTGAAGCAGAACTATGGTGAAAAAGTTCTTGTACAGTTTGAAGATTTTGCTAACCACAATGCCTTTGAGCTGCTGGCAAAATATGGAACTACTCATCTCGTCTTCAATGATGACATACAGGGTACGGCTGCTGTAGTTCTTGCTGGAGTTGTGGCGGCACTAAAGTTGATCAGTGGTACGCTGTCTGAGCACAAATTTTTGTTCCTTGGTGCTGGGGAAGCTGGGACAGGTATAGCAGAGCTAATAGCTCTTGAGATCTCAAAAAAGACAAAGATTCCAGTGGAAGAGACGCGCAAAAAGATCTGGCTTGTTGATTCAAAGGGACTAATTGTTAGCTCACGTAAAGAATCTCTTCAACATTTTAAGAAGCCTTGGGCTCATGAACATGAACCTGTTAAGGATCTCATTGATGCTGTCAAGGCAATCAAACCAACAGTTCTTATTGGATCGTCTGGTGTTGGAAGAACATTCACGAAAGAAGTGATCGAGGCACTAGCTTCCTTCAATGAGAAACCTCTCATTTTGGCTCTTTCTAATCCAACATCACAGTCAGAATGTACAGCTGAAGAAGCTTATACTTGGACTAAGGGTCGTGCAATTTTTGCTAGTGGAAGTCCTTTTGATCCTGTTGAATATAATGGCAAAGTTTATGTTCCTGGCCAGTCCAACAATGCTTACATTTTCCCCGGACTTGGCCTTGGTTTGGTCATCTCTGGAGCAATCCGTGTGCATGATGATATGCTTCTAGCAGCCTCCGAGGCCTTGGCGGGTCAAGTTACAAAGGAGAATATCGATAATGGACTTATCTATCCACCATTCTCTAAAATCAGAAAGATTTCTGCCGCAATAGCTGCTAATGTAGCTGCCAAGGCATATGAACTTGGTGTTGCTACACGACTCCCTCGTCCAGAAAATCTTGTCAAGCATGCTGAGAGTTGCATGTATAGTCCTCTCTACAGAAGCTACCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006090 GO:0006108 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0055114 GO:0065003 GO:0071704 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

641

Amino Acids

70.86

Weight (kDa)

8.83

Isoelectric Point (pI)

37.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	116 - 296	1.9e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	307 - 560	3.9e-97	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19900 AT2G19900
fragaria_vesca	FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000
malus_domestica	MD02G1064800.v1.1 MD15G1195500.v1.1
prunus_persica	Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1
pyrus_communis	pycom02g05090 pycom15g17470
rosa_chinensis	RchiOBHm_Chr2g0091741
rosa_laevigata	RLG00000016248
rosa_roxburghii	Rroxscaffold_2G00149780
rosa_rugosa	Rorug02G0020300
rosa_samantha	Rh2BG064200 Rh2CG065800 Rh2DG064300
rosa_wichuraiana	Rw2G005550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	269, 1854
AccB7I	CCANNNNNTGG	1 cut(s)	575
AccBSI	CCGCTC	1 cut(s)	33
AccI	GTMKAC	1 cut(s)	788
AccII	CGCG	1 cut(s)	1243
AciI	CCGC	8 cut(s)	33, 73, 210, 222, 461, 1106, 1718, 1794
AclWI	GGATC	4 cut(s)	314, 1359, 1405, 1568
AcoI	YGGCCR	2 cut(s)	34, 1609
AcsI	RAATTY	4 cut(s)	654, 762, 875, 1145
AcuI	CTGAAG	4 cut(s)	401, 592, 887, 1536
AcyI	GRCGYC	1 cut(s)	613
AfaI	GTAC	4 cut(s)	984, 1075, 1129, 1510
AfiI	CCNNNNNNNGG	3 cut(s)	575, 1643, 1654
AflII	CTTAAG	1 cut(s)	161
AflIII	ACRYGT	1 cut(s)	818
AgeI	ACCGGT	1 cut(s)	1917
AgsI	TTSAA	8 cut(s)	670, 695, 992, 1057, 1269, 1307, 1456, 1583
AhdI	GACNNNNNGTC	1 cut(s)	1539
AjnI	CCWGG	4 cut(s)	195, 278, 492, 1606
AjuI	GAANNNNNNNTTGG	2 cut(s)	867, 899
Alw21I	GWGCWC	1 cut(s)	1143
Alw26I	GTCTC	2 cut(s)	49, 1232
AlwI	GGATC	4 cut(s)	314, 1359, 1405, 1568
Ama87I	CYCGRG	1 cut(s)	659
AoxI	GGCC	5 cut(s)	34, 677, 1609, 1645, 1710
ApeKI	GCWGC	7 cut(s)	251, 463, 1021, 1079, 1700, 1802, 1814
ApoI	RAATTY	4 cut(s)	654, 762, 875, 1145
ArsI	GACNNNNNNTTYG	2 cut(s)	198, 230
AseI	ATTAAT	1 cut(s)	11
AsiGI	ACCGGT	1 cut(s)	1917
Asp700I	GAANNNNTTC	4 cut(s)	49, 975, 1302, 1786
AspLEI	GCGC	2 cut(s)	950, 1245
AspS9I	GGNCC	1 cut(s)	262
AsuC2I	CCSGG	2 cut(s)	21, 1638
AsuHPI	GGTGA	6 cut(s)	254, 265, 590, 728, 749, 980
AvaI	CYCGRG	1 cut(s)	659
AvaII	GGWCC	1 cut(s)	262
AxyI	CCTNAGG	1 cut(s)	618
BalI	TGGCCA	1 cut(s)	1611
BanII	GRGCYC	2 cut(s)	891, 1330
BbsI	GAAGAC	2 cut(s)	377, 1045
Bbv12I	GWGCWC	1 cut(s)	1143
BbvI	GCAGC	7 cut(s)	238, 450, 1008, 1066, 1712, 1789, 1801
BccI	CCATC	3 cut(s)	170, 707, 751
BceAI	ACGGC	3 cut(s)	21, 197, 1092
BciT130I	CCWGG	4 cut(s)	197, 280, 494, 1608
BclI	TGATCA	1 cut(s)	1119
BcnI	CCSGG	2 cut(s)	21, 1638
BcoDI	GTCTC	2 cut(s)	49, 1232
BfaI	CTAG	4 cut(s)	140, 1445, 1557, 1697
BfmI	CTRYAG	2 cut(s)	1083, 1906
BfoI	RGCGCY	1 cut(s)	951
BfrI	CTTAAG	1 cut(s)	161
BglI	GCCNNNNNGGC	1 cut(s)	1709
BglII	AGATCT	2 cut(s)	1206, 1251
BlpI	GCTNAGC	1 cut(s)	924
Bme1390I	CCNGG	6 cut(s)	21, 197, 280, 494, 1608, 1638
Bme18I	GGWCC	1 cut(s)	262
BmeRI	GACNNNNNGTC	1 cut(s)	1539
BmeT110I	CYCGRG	1 cut(s)	659
BmgT120I	GGNCC	1 cut(s)	262
BmrFI	CCNGG	6 cut(s)	21, 197, 280, 494, 1608, 1638
BmsI	GCATC	5 cut(s)	238, 355, 423, 612, 1351
BpiI	GAAGAC	2 cut(s)	377, 1045
BplI	GAGNNNNNCTC	4 cut(s)	307, 339, 352, 384
BpmI	CTGGAG	3 cut(s)	476, 1116, 1686
Bpu10I	CCTNAGC	1 cut(s)	890
Bpu1102I	GCTNAGC	1 cut(s)	924
BpuEI	CTTGAG	1 cut(s)	1223
BpuMI	CCSGG	2 cut(s)	21, 1638
Bsa29I	ATCGAT	1 cut(s)	1743
BsaAI	YACGTR	1 cut(s)	1292
BsaHI	GRCGYC	1 cut(s)	613
BsaWI	WCCGGW	1 cut(s)	1917
BsaXI	ACNNNNNCTCC	2 cut(s)	185, 215
Bsc4I	CCNNNNNNNGG	3 cut(s)	575, 1643, 1654
Bse118I	RCCGGY	1 cut(s)	1917
Bse1I	ACTGG	6 cut(s)	297, 405, 680, 913, 1229, 1612
Bse21I	CCTNAGG	1 cut(s)	618
BseBI	CCWGG	4 cut(s)	197, 280, 494, 1608
BseCI	ATCGAT	1 cut(s)	1743
BseGI	GGATG	5 cut(s)	181, 253, 370, 762, 877
BseLI	CCNNNNNNNGG	3 cut(s)	575, 1643, 1654
BseNI	ACTGG	6 cut(s)	297, 405, 680, 913, 1229, 1612
BseRI	GAGGAG	1 cut(s)	816
BseX3I	CGGCCG	1 cut(s)	34
BseXI	GCAGC	7 cut(s)	238, 450, 1008, 1066, 1712, 1789, 1801
BseYI	CCCAGC	2 cut(s)	1163, 1172
BsgI	GTGCAG	1 cut(s)	171
Bsh1236I	CGCG	1 cut(s)	1243
Bsh1285I	CGRYCG	1 cut(s)	37
BshFI	GGCC	5 cut(s)	36, 679, 1611, 1647, 1712
BshTI	ACCGGT	1 cut(s)	1917
BshVI	ATCGAT	1 cut(s)	1743
BsiEI	CGRYCG	1 cut(s)	37
BsiHKAI	GWGCWC	1 cut(s)	1143
BsiHKCI	CYCGRG	1 cut(s)	659
BsiSI	CCGG	4 cut(s)	21, 395, 1638, 1918
BslFI	GGGAC	5 cut(s)	744, 795, 859, 1189, 1287
BslI	CCNNNNNNNGG	3 cut(s)	575, 1643, 1654
BsmAI	GTCTC	2 cut(s)	49, 1232
BsmBI	CGTCTC	1 cut(s)	1232
BsmFI	GGGAC	5 cut(s)	744, 795, 859, 1189, 1287
BsnI	GGCC	5 cut(s)	36, 679, 1611, 1647, 1712
BsoBI	CYCGRG	1 cut(s)	659
Bsp1286I	GDGCHC	3 cut(s)	891, 1143, 1330
Bsp1407I	TGTACA	2 cut(s)	982, 1508
Bsp143I	GATC	8 cut(s)	319, 1119, 1206, 1251, 1351, 1397, 1434, 1573
Bsp1720I	GCTNAGC	1 cut(s)	924
BspACI	CCGC	8 cut(s)	33, 73, 210, 222, 461, 1106, 1718, 1794
BspANI	GGCC	5 cut(s)	36, 679, 1611, 1647, 1712
BspDI	ATCGAT	1 cut(s)	1743
BspFNI	CGCG	1 cut(s)	1243
BspHI	TCATGA	1 cut(s)	1330
BspPI	GGATC	4 cut(s)	314, 1359, 1405, 1568
BspTI	CTTAAG	1 cut(s)	161
BsrBI	CCGCTC	1 cut(s)	33
BsrFI	RCCGGY	1 cut(s)	1917
BsrGI	TGTACA	2 cut(s)	982, 1508
BsrI	ACTGG	6 cut(s)	297, 405, 680, 913, 1229, 1612
BssAI	RCCGGY	1 cut(s)	1917
BssMI	GATC	8 cut(s)	319, 1119, 1206, 1251, 1351, 1397, 1434, 1573
BssNAI	GTATAC	1 cut(s)	789
BssNI	GRCGYC	1 cut(s)	613
BssT1I	CCWWGG	6 cut(s)	742, 1156, 1322, 1648, 1713, 1818
Bst1107I	GTATAC	1 cut(s)	789
Bst2UI	CCWGG	4 cut(s)	197, 280, 494, 1608
Bst4CI	ACNGT	5 cut(s)	242, 574, 987, 1387, 1500
Bst6I	CTCTTC	2 cut(s)	1230, 1308
BstACI	GRCGYC	1 cut(s)	613
BstAFI	CTTAAG	1 cut(s)	161
BstAPI	GCANNNNNTGC	2 cut(s)	1553, 1802
BstAUI	TGTACA	2 cut(s)	982, 1508
BstBAI	YACGTR	1 cut(s)	1292
BstC8I	GCNNGC	4 cut(s)	342, 586, 1026, 1878
BstDSI	CCRYGG	1 cut(s)	37
BstEII	GGTNACC	1 cut(s)	737
BstF5I	GGATG	5 cut(s)	181, 253, 370, 762, 877
BstFNI	CGCG	1 cut(s)	1243
BstH2I	RGCGCY	1 cut(s)	951
BstHHI	GCGC	2 cut(s)	950, 1245
BstKTI	GATC	8 cut(s)	322, 1122, 1209, 1254, 1354, 1400, 1437, 1576
BstMAI	GTCTC	2 cut(s)	49, 1232
BstMBI	GATC	8 cut(s)	319, 1119, 1206, 1251, 1351, 1397, 1434, 1573
BstMCI	CGRYCG	1 cut(s)	37
BstNI	CCWGG	4 cut(s)	197, 280, 494, 1608
BstNSI	RCATGY	3 cut(s)	822, 1880, 1894
BstPI	GGTNACC	1 cut(s)	737
BstSCI	CCNGG	6 cut(s)	19, 195, 278, 492, 1606, 1636
BstSFI	CTRYAG	2 cut(s)	1083, 1906
BstUI	CGCG	1 cut(s)	1243
BstV1I	GCAGC	7 cut(s)	238, 450, 1008, 1066, 1712, 1789, 1801
BstV2I	GAAGAC	2 cut(s)	377, 1045
BstX2I	RGATCY	4 cut(s)	319, 1206, 1251, 1351
BstXI	CCANNNNNNTGG	2 cut(s)	596, 841
BstYI	RGATCY	4 cut(s)	319, 1206, 1251, 1351
BstZ17I	GTATAC	1 cut(s)	789
BstZI	CGGCCG	1 cut(s)	34
Bsu15I	ATCGAT	1 cut(s)	1743
Bsu36I	CCTNAGG	1 cut(s)	618
BsuRI	GGCC	5 cut(s)	36, 679, 1611, 1647, 1712
BsuTUI	ATCGAT	1 cut(s)	1743
BtgI	CCRYGG	1 cut(s)	37
BtsCI	GGATG	5 cut(s)	181, 253, 370, 762, 877
BtsIMutI	CAGTG	5 cut(s)	255, 304, 479, 1129, 1236
Cac8I	GCNNGC	4 cut(s)	342, 586, 1026, 1878
CciI	TCATGA	1 cut(s)	1330
CfoI	GCGC	2 cut(s)	950, 1245
Cfr10I	RCCGGY	1 cut(s)	1917
Cfr13I	GGNCC	1 cut(s)	262
ClaI	ATCGAT	1 cut(s)	1743
CseI	GACGC	2 cut(s)	621, 1249
Csp6I	GTAC	4 cut(s)	983, 1074, 1128, 1509
CspAI	ACCGGT	1 cut(s)	1917
CviAII	CATG	8 cut(s)	819, 935, 943, 1331, 1337, 1682, 1877, 1891
CviQI	GTAC	4 cut(s)	983, 1074, 1128, 1509
DpnI	GATC	8 cut(s)	321, 1121, 1208, 1253, 1353, 1399, 1436, 1575
DpnII	GATC	8 cut(s)	319, 1119, 1206, 1251, 1351, 1397, 1434, 1573
DrdI	GACNNNNNNGTC	2 cut(s)	269, 1854
DriI	GACNNNNNGTC	1 cut(s)	1539
DseDI	GACNNNNNNGTC	2 cut(s)	269, 1854
EaeI	YGGCCR	2 cut(s)	34, 1609
EagI	CGGCCG	1 cut(s)	34
Eam1104I	CTCTTC	2 cut(s)	1230, 1308
Eam1105I	GACNNNNNGTC	1 cut(s)	1539
EarI	CTCTTC	2 cut(s)	1230, 1308
EciI	GGCGGA	1 cut(s)	199
EclXI	CGGCCG	1 cut(s)	34
Eco130I	CCWWGG	6 cut(s)	742, 1156, 1322, 1648, 1713, 1818
Eco147I	AGGCCT	1 cut(s)	1712
Eco24I	GRGCYC	2 cut(s)	891, 1330
Eco47I	GGWCC	1 cut(s)	262
Eco52I	CGGCCG	1 cut(s)	34
Eco57I	CTGAAG	4 cut(s)	401, 592, 887, 1536
Eco81I	CCTNAGG	1 cut(s)	618
Eco88I	CYCGRG	1 cut(s)	659
Eco91I	GGTNACC	1 cut(s)	737
EcoO65I	GGTNACC	1 cut(s)	737
EcoRI	GAATTC	3 cut(s)	654, 762, 875
EcoRII	CCWGG	4 cut(s)	195, 278, 492, 1606
EcoT14I	CCWWGG	6 cut(s)	742, 1156, 1322, 1648, 1713, 1818
EcoT38I	GRGCYC	2 cut(s)	891, 1330
ErhI	CCWWGG	6 cut(s)	742, 1156, 1322, 1648, 1713, 1818
Esp3I	CGTCTC	1 cut(s)	1232
FaeI	CATG	8 cut(s)	822, 938, 946, 1334, 1340, 1685, 1880, 1894
FalI	AAGNNNNNCTT	4 cut(s)	116, 148, 1287, 1319
FaqI	GGGAC	5 cut(s)	744, 795, 859, 1189, 1287
FatI	CATG	8 cut(s)	818, 934, 942, 1330, 1336, 1681, 1876, 1890
FauI	CCCGC	1 cut(s)	1711
FauNDI	CATATG	1 cut(s)	1825
FbaI	TGATCA	1 cut(s)	1119
FblI	GTMKAC	1 cut(s)	788
FokI	GGATG	5 cut(s)	188, 260, 377, 769, 884
FriOI	GRGCYC	2 cut(s)	891, 1330
FspBI	CTAG	4 cut(s)	140, 1445, 1557, 1697
GlaI	GCGC	2 cut(s)	949, 1244
GsaI	CCCAGC	2 cut(s)	1167, 1176
GsuI	CTGGAG	3 cut(s)	476, 1116, 1686
HaeII	RGCGCY	1 cut(s)	951
HaeIII	GGCC	5 cut(s)	36, 679, 1611, 1647, 1712
HapII	CCGG	4 cut(s)	21, 395, 1638, 1918
HgaI	GACGC	2 cut(s)	621, 1249
HhaI	GCGC	2 cut(s)	950, 1245
Hin1I	GRCGYC	1 cut(s)	613
Hin1II	CATG	8 cut(s)	822, 938, 946, 1334, 1340, 1685, 1880, 1894
Hin6I	GCGC	2 cut(s)	948, 1243
HinP1I	GCGC	2 cut(s)	948, 1243
HindIII	AAGCTT	1 cut(s)	1521
HinfI	GANTC	6 cut(s)	136, 200, 1225, 1265, 1298, 1848
HpaII	CCGG	4 cut(s)	21, 395, 1638, 1918
HphI	GGTGA	6 cut(s)	254, 265, 590, 728, 749, 980
Hpy166II	GTNNAC	2 cut(s)	41, 789
Hpy188I	TCNGA	7 cut(s)	446, 611, 639, 1138, 1504, 1708, 1781
Hpy8I	GTNNAC	2 cut(s)	41, 789
Hpy99I	CGWCG	1 cut(s)	218
HpyAV	CCTTC	4 cut(s)	93, 97, 862, 1462
HpyCH4III	ACNGT	5 cut(s)	242, 574, 987, 1387, 1500
HpyCH4IV	ACGT	1 cut(s)	1291
HpyCH4V	TGCA	7 cut(s)	188, 344, 436, 466, 1547, 1681, 1890
HpySE526I	ACGT	1 cut(s)	1291
Hsp92I	GRCGYC	1 cut(s)	613
Hsp92II	CATG	8 cut(s)	822, 938, 946, 1334, 1340, 1685, 1880, 1894
HspAI	GCGC	2 cut(s)	948, 1243
Ksp22I	TGATCA	1 cut(s)	1119
Kzo9I	GATC	8 cut(s)	319, 1119, 1206, 1251, 1351, 1397, 1434, 1573
LmnI	GCTCC	2 cut(s)	555, 1667
Lsp1109I	GCAGC	7 cut(s)	238, 450, 1008, 1066, 1712, 1789, 1801
LweI	GCATC	5 cut(s)	238, 355, 423, 612, 1351
MaeI	CTAG	4 cut(s)	140, 1445, 1557, 1697
MaeII	ACGT	1 cut(s)	1291
MaeIII	GTNAC	4 cut(s)	271, 706, 737, 1726
MalI	GATC	8 cut(s)	321, 1121, 1208, 1253, 1353, 1399, 1436, 1575
MbiI	CCGCTC	1 cut(s)	33
MboI	GATC	8 cut(s)	319, 1119, 1206, 1251, 1351, 1397, 1434, 1573
MfeI	CAATTG	1 cut(s)	860
MflI	RGATCY	4 cut(s)	319, 1206, 1251, 1351
MhlI	GDGCHC	3 cut(s)	891, 1143, 1330
MlsI	TGGCCA	1 cut(s)	1611
MluCI	AATT	9 cut(s)	545, 654, 762, 776, 860, 875, 1145, 1278, 1548
MluNI	TGGCCA	1 cut(s)	1611
MlyI	GAGTC	3 cut(s)	130, 194, 1842
MmeI	TCCRAC	4 cut(s)	593, 1390, 1514, 1641
Mox20I	TGGCCA	1 cut(s)	1611
MroXI	GAANNNNTTC	4 cut(s)	49, 975, 1302, 1786
MscI	TGGCCA	1 cut(s)	1611
MseI	TTAA	4 cut(s)	11, 162, 1314, 1347
MslI	CAYNNNNRTG	2 cut(s)	839, 1335
Msp20I	TGGCCA	1 cut(s)	1611
MspA1I	CMGCKG	2 cut(s)	463, 1514
MspCI	CTTAAG	1 cut(s)	161
MspI	CCGG	4 cut(s)	21, 395, 1638, 1918
MspR9I	CCNGG	6 cut(s)	21, 197, 280, 494, 1608, 1638
MunI	CAATTG	1 cut(s)	860
MvaI	CCWGG	4 cut(s)	197, 280, 494, 1608
MvnI	CGCG	1 cut(s)	1243
NciI	CCSGG	2 cut(s)	21, 1638
NdeI	CATATG	1 cut(s)	1825
NdeII	GATC	8 cut(s)	319, 1119, 1206, 1251, 1351, 1397, 1434, 1573
NlaIII	CATG	8 cut(s)	822, 938, 946, 1334, 1340, 1685, 1880, 1894
NmuCI	GTSAC	2 cut(s)	271, 737
NspI	RCATGY	3 cut(s)	822, 1880, 1894
PaeI	GCATGC	1 cut(s)	1880
PaeR7I	CTCGAG	1 cut(s)	659
PagI	TCATGA	1 cut(s)	1330
PceI	AGGCCT	1 cut(s)	1712
PciI	ACATGT	1 cut(s)	818
PcsI	WCGNNNNNNNCGW	2 cut(s)	222, 1852
PdmI	GAANNNNTTC	4 cut(s)	49, 975, 1302, 1786
PfeI	GAWTC	3 cut(s)	1225, 1265, 1298
PflFI	GACNNNGTC	1 cut(s)	536
PflMI	CCANNNNNTGG	1 cut(s)	575
PfoI	TCCNGGA	1 cut(s)	492
PinAI	ACCGGT	1 cut(s)	1917
PleI	GAGTC	3 cut(s)	130, 194, 1842
PpsI	GAGTC	3 cut(s)	130, 194, 1842
Ppu21I	YACGTR	1 cut(s)	1292
PscI	ACATGT	1 cut(s)	818
PshBI	ATTAAT	1 cut(s)	11
Psp6I	CCWGG	4 cut(s)	195, 278, 492, 1606
PspEI	GGTNACC	1 cut(s)	737
PspFI	CCCAGC	2 cut(s)	1163, 1172
PspGI	CCWGG	4 cut(s)	195, 278, 492, 1606
PspPI	GGNCC	1 cut(s)	262
PsuI	RGATCY	4 cut(s)	319, 1206, 1251, 1351
PsyI	GACNNNGTC	1 cut(s)	536
PvuII	CAGCTG	1 cut(s)	1514
RsaI	GTAC	4 cut(s)	984, 1075, 1129, 1510
RsaNI	GTAC	4 cut(s)	983, 1074, 1128, 1509
RseI	CAYNNNNRTG	2 cut(s)	839, 1335
SaqAI	TTAA	4 cut(s)	11, 162, 1314, 1347
Sau3AI	GATC	8 cut(s)	319, 1119, 1206, 1251, 1351, 1397, 1434, 1573
Sau96I	GGNCC	1 cut(s)	262
SchI	GAGTC	3 cut(s)	130, 194, 1842
ScrFI	CCNGG	6 cut(s)	21, 197, 280, 494, 1608, 1638
SduI	GDGCHC	3 cut(s)	891, 1143, 1330
SfaNI	GCATC	5 cut(s)	238, 355, 423, 612, 1351
SfcI	CTRYAG	2 cut(s)	1083, 1906
Sfr274I	CTCGAG	1 cut(s)	659
SinI	GGWCC	1 cut(s)	262
SlaI	CTCGAG	1 cut(s)	659
SmiMI	CAYNNNNRTG	2 cut(s)	839, 1335
SmlI	CTYRAG	3 cut(s)	161, 659, 1202
SmoI	CTYRAG	3 cut(s)	161, 659, 1202
SphI	GCATGC	1 cut(s)	1880
Sse9I	AATT	9 cut(s)	545, 654, 762, 776, 860, 875, 1145, 1278, 1548
SseBI	AGGCCT	1 cut(s)	1712
SsiI	CCGC	8 cut(s)	33, 73, 210, 222, 461, 1106, 1718, 1794
SspMI	CTAG	4 cut(s)	140, 1445, 1557, 1697
StuI	AGGCCT	1 cut(s)	1712
StyD4I	CCNGG	6 cut(s)	19, 195, 278, 492, 1606, 1636
StyI	CCWWGG	6 cut(s)	742, 1156, 1322, 1648, 1713, 1818
TaaI	ACNGT	5 cut(s)	242, 574, 987, 1387, 1500
TaiI	ACGT	1 cut(s)	1294
TaqI	TCGA	5 cut(s)	53, 540, 660, 1437, 1743
TaqII	GACCGA	1 cut(s)	500
TasI	AATT	9 cut(s)	545, 654, 762, 776, 860, 875, 1145, 1278, 1548
TatI	WGTACW	2 cut(s)	982, 1508
TauI	GCSGC	5 cut(s)	36, 225, 463, 1109, 1796
TfiI	GAWTC	3 cut(s)	1225, 1265, 1298
Tru1I	TTAA	4 cut(s)	11, 162, 1314, 1347
Tru9I	TTAA	4 cut(s)	11, 162, 1314, 1347
TscAI	CASTG	5 cut(s)	262, 304, 486, 1129, 1236
TseFI	GTSAC	2 cut(s)	271, 737
TseI	GCWGC	7 cut(s)	251, 463, 1021, 1079, 1700, 1802, 1814
Tsp45I	GTSAC	2 cut(s)	271, 737
TspDTI	ATGAA	6 cut(s)	870, 888, 931, 1347, 1353, 1842
TspGWI	ACGGA	1 cut(s)	1664
TspRI	CASTG	5 cut(s)	262, 304, 486, 1129, 1236
Tth111I	GACNNNGTC	1 cut(s)	536
Van91I	CCANNNNNTGG	1 cut(s)	575
Vha464I	CTTAAG	1 cut(s)	161
VpaK11BI	GGWCC	1 cut(s)	262
VspI	ATTAAT	1 cut(s)	11
XapI	RAATTY	4 cut(s)	654, 762, 875, 1145
XceI	RCATGY	3 cut(s)	822, 1880, 1894
XhoI	CTCGAG	1 cut(s)	659
XmiI	GTMKAC	1 cut(s)	788
XmnI	GAANNNNTTC	4 cut(s)	49, 975, 1302, 1786
XspI	CTAG	4 cut(s)	140, 1445, 1557, 1697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.