Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005972

Physical Location & Seq

Genomic Coordinates Forward (+)

5252605 .. 5257937

Gene Structure 5333 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1064800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGCGAGCAAGCTCACGGATCCTACGGTTAAGATCGACCCGAAATCCACCGTCGGCGGCGGCGTTGAGGATGTATACGGTGAGGACACTGCCACCGAGGACCAACTTATCACCCCCTGGACTTACTCCGTTGCCAGTGGGTATTCGTTGCTGAGAGATCCTCAATACAACAAAGGGCTAGCATTTACAGAGAAAGAGAGGGACGCCCATTACTTGCGCGGTCTTCTTCCTCCGGCAACTATTTCTCAACAACTCCAGGAGAAGAAGTTGATGAATACCATCCGACAATATCAAGTCCCGTTGCAAAAATATATGGCACTGACGGAACTCCAGGAGAGGAATGAGAGGCTGTTCTACAAACTTCTCATTGATAATGTCGAGGAATTGCTCCCAATTGTCTACACTCCGACTGTTGGTGAGGCTTGCCAGAAATTCGGAAGCATCTTCAGGCGCCCTCAGGGTCTTTACATAAGTCTAAAAGAGAAGGGTAAAGTTCTCGAGGTATTGAAGAACTGGCCTGTAAGGACTGTTCAAGTTATTGTTGTGACTGATGGTGAACGTATCCTGGGACTTGGTGACCTTGGTTGTCAGGGGATGGGAATTCCTGTGGGGAAGTTGGCTTTGTACACGGCACTTGGAGGAGTTCGCCCCTCAATGTGTTTGCCTGTAACAATTGATGTTGGGACAAACAATGAGAAGTTGCTGGATAATGAGTTTTACATTGGACTCAGACAAAAGAGGACAAGGGGAAAGGAGTACGAGGATCTTTTAGAAGAGTTCATGAGTGCTGTCAAGCAGAACTATGGTGAAAAAGTTATTGTACAGTTCGAAGATTTTGCAAACCACAATGCTTTTGAGCTGCTGGCCAAATATAGAGCATCTCATCTCGTCTTCAATGATGACATACAGGGGACGGCATCTGTAGTTCTTGCAGGAGTTGTGGCAGCACTGAAGTTAATCGGTGGTTCCCTGTCTGAGCATAGGTTTTTGTTCCTTGGCGCTGGAGAAGCTGGTACCGGTATAGCAGAACTAATAGCTCTTGAGATCTCGAAAAGGACAAAAGTTCCTGTGGAAGAGACCCGTAAGAAGATCTGGCTTGTTGATTCAAAGGGATTGATTGTTATCTCTCGTAAAGATTCTCTTCAACATTTTAAGCAGCCTTGGGCTCATGAGCATGAGCCTGTTGATAATCTTTTGGATGCTGTCAAGGCAATTAAACCAACAGTTCTTATTGGATCGTCTGGTGTTGGAAGAACATTTACAAAGGAGGTGATTGAAGCAGTATCGTCCTTCAATGAGAAACCTCTCATTTTGGCTCTTTCCAACCCAACATCACAATCTGAATGTACCGCTGAAGAAGCTTATACTTACAGTGAGGGTCGTGCAATTTTTGCCAGCGGAAGTCCATTTGATCCTTTTGAGTACAAGGGCAAAGTTTTTGTTCCTGGCCAGTCCAACAATGCATACATTTTTCCTGGATTTGGTCTGGGTTTGGTTATCTCTGGAGCAATCCGTGTTCACGATGACATGCTTCTGGCAGCCTCGGAAGCCTTGGCGGGGCAAGTTTCCGAGGAGAATTTTGCAAAGGGGCTGATCTATCCACCATTGTCGAACATCAGAAAGATTTCTGCCCAAGTAGCTGCTAATGTAGCTGCCAAGGCATATGACCTTGGCGTGGCTACGCGTCTCCCTCGTCCTGAGAATCTTGTGAAGCATGCCGAGAGTTGCATGTACAGTCCCGTCTACAGAAACTACCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006090 GO:0006108 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0055114 GO:0065003 GO:0071704 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

587

Amino Acids

64.7

Weight (kDa)

6.38

Isoelectric Point (pI)

38.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	111 - 292	3.5e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	302 - 555	8.8e-94	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19900 AT2G19900
fragaria_vesca	FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000
malus_domestica	MD02G1064800.v1.1 MD15G1195500.v1.1
prunus_persica	Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1
pyrus_communis	pycom02g05090 pycom15g17470
rosa_chinensis	RchiOBHm_Chr2g0091741
rosa_laevigata	RLG00000016248
rosa_roxburghii	Rroxscaffold_2G00149780
rosa_rugosa	Rorug02G0020300
rosa_samantha	Rh2BG064200 Rh2CG065800 Rh2DG064300
rosa_wichuraiana	Rw2G005550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1013
AccB1I	GGYRCC	2 cut(s)	450, 1013
AccI	GTMKAC	3 cut(s)	75, 399, 1743
AccII	CGCG	2 cut(s)	219, 1684
AciI	CCGC	7 cut(s)	57, 60, 219, 1350, 1398, 1556, 1756
AclWI	GGATC	6 cut(s)	14, 27, 152, 771, 1243, 1406
AcoI	YGGCCR	2 cut(s)	864, 1447
AcsI	RAATTY	3 cut(s)	431, 600, 1576
AcuI	CTGAAG	3 cut(s)	430, 971, 1374
AcyI	GRCGYC	2 cut(s)	204, 451
AfaI	GTAC	7 cut(s)	626, 758, 822, 1015, 1348, 1424, 1733
AfiI	CCNNNNNNNGG	4 cut(s)	413, 1481, 1557, 1675
AflIII	ACRYGT	1 cut(s)	1682
AgeI	ACCGGT	1 cut(s)	1016
AgsI	TTSAA	7 cut(s)	508, 533, 895, 1107, 1145, 1277, 1294
AjnI	CCWGG	6 cut(s)	116, 255, 330, 564, 1444, 1474
AluBI	AGCT	7 cut(s)	13, 859, 1010, 1037, 1361, 1640, 1652
AluI	AGCT	7 cut(s)	13, 859, 1010, 1037, 1361, 1640, 1652
Alw26I	GTCTC	2 cut(s)	1070, 1691
AlwI	GGATC	6 cut(s)	14, 27, 152, 771, 1243, 1406
Ama87I	CYCGRG	1 cut(s)	497
AoxI	GGCC	3 cut(s)	515, 864, 1447
ApeKI	GCWGC	6 cut(s)	859, 944, 1156, 1538, 1640, 1652
ApoI	RAATTY	3 cut(s)	431, 600, 1576
AsiGI	ACCGGT	1 cut(s)	1016
Asp700I	GAANNNNTTC	1 cut(s)	1624
Asp718I	GGTACC	1 cut(s)	1013
AspLEI	GCGC	3 cut(s)	219, 453, 1001
AspS9I	GGNCC	1 cut(s)	100
AsuHPI	GGTGA	7 cut(s)	92, 103, 428, 566, 587, 818, 1282
AsuII	TTCGAA	1 cut(s)	828
AsuNHI	GCTAGC	1 cut(s)	178
AvaI	CYCGRG	1 cut(s)	497
AvaII	GGWCC	1 cut(s)	100
AxyI	CCTNAGG	1 cut(s)	456
BalI	TGGCCA	2 cut(s)	866, 1449
BamHI	GGATCC	1 cut(s)	19
BanI	GGYRCC	2 cut(s)	450, 1013
BanII	GRGCYC	1 cut(s)	1168
BarI	GAAGNNNNNNTAC	2 cut(s)	1243, 1275
BbsI	GAAGAC	2 cut(s)	215, 883
BbvI	GCAGC	6 cut(s)	846, 956, 1168, 1550, 1627, 1639
BccI	CCATC	3 cut(s)	287, 545, 589
BceAI	ACGGC	2 cut(s)	645, 930
BcgI	CGANNNNNNTGC	2 cut(s)	818, 852
BciT130I	CCWGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
BciVI	GTATCC	1 cut(s)	572
BcoDI	GTCTC	2 cut(s)	1070, 1691
BfaI	CTAG	1 cut(s)	179
BfmI	CTRYAG	2 cut(s)	921, 1744
BfoI	RGCGCY	2 cut(s)	454, 1002
BfuI	GTATCC	1 cut(s)	572
BglII	AGATCT	2 cut(s)	1044, 1089
BisI	GCNGC	8 cut(s)	58, 61, 860, 945, 1157, 1539, 1641, 1653
BlsI	GCNGC	8 cut(s)	59, 62, 861, 946, 1158, 1540, 1642, 1654
Bme1390I	CCNGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
Bme18I	GGWCC	1 cut(s)	100
BmeT110I	CYCGRG	1 cut(s)	497
BmgT120I	GGNCC	1 cut(s)	100
BmiI	GGNNCC	4 cut(s)	21, 452, 967, 1015
BmrFI	CCNGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
BmsI	GCATC	4 cut(s)	450, 887, 926, 1189
BmtI	GCTAGC	1 cut(s)	182
BpiI	GAAGAC	2 cut(s)	215, 883
BplI	GAGNNNNNCTC	2 cut(s)	145, 177
BpmI	CTGGAG	4 cut(s)	239, 314, 1023, 1524
Bpu14I	TTCGAA	1 cut(s)	828
BpuEI	CTTGAG	1 cut(s)	1061
BsaBI	GATNNNNATC	1 cut(s)	1121
BsaHI	GRCGYC	2 cut(s)	204, 451
BsaI	GGTCTC	1 cut(s)	1070
BsaWI	WCCGGW	1 cut(s)	1016
BsaXI	ACNNNNNCTCC	2 cut(s)	251, 281
Bsc4I	CCNNNNNNNGG	4 cut(s)	413, 1481, 1557, 1675
Bse118I	RCCGGY	1 cut(s)	1016
Bse1I	ACTGG	3 cut(s)	135, 518, 1450
Bse21I	CCTNAGG	1 cut(s)	456
Bse8I	GATNNNNATC	1 cut(s)	1121
BseBI	CCWGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
BseGI	GGATG	4 cut(s)	76, 279, 600, 1204
BseJI	GATNNNNATC	1 cut(s)	1121
BseLI	CCNNNNNNNGG	4 cut(s)	413, 1481, 1557, 1675
BseMII	CTCAG	5 cut(s)	143, 470, 742, 966, 1689
BseNI	ACTGG	3 cut(s)	135, 518, 1450
BseRI	GAGGAG	2 cut(s)	654, 1586
BseXI	GCAGC	6 cut(s)	846, 956, 1168, 1550, 1627, 1639
Bsh1236I	CGCG	2 cut(s)	219, 1684
BshFI	GGCC	3 cut(s)	517, 866, 1449
BshNI	GGYRCC	2 cut(s)	450, 1013
BshTI	ACCGGT	1 cut(s)	1016
BsiHKCI	CYCGRG	1 cut(s)	497
BsiSI	CCGG	2 cut(s)	233, 1017
BslFI	GGGAC	6 cut(s)	215, 281, 582, 697, 925, 1722
BslI	CCNNNNNNNGG	4 cut(s)	413, 1481, 1557, 1675
BsmAI	GTCTC	2 cut(s)	1070, 1691
BsmBI	CGTCTC	1 cut(s)	1691
BsmFI	GGGAC	6 cut(s)	215, 281, 582, 697, 925, 1722
BsnI	GGCC	3 cut(s)	517, 866, 1449
Bso31I	GGTCTC	1 cut(s)	1070
BsoBI	CYCGRG	1 cut(s)	497
Bsp119I	TTCGAA	1 cut(s)	828
Bsp1286I	GDGCHC	1 cut(s)	1168
Bsp1407I	TGTACA	3 cut(s)	624, 820, 1731
Bsp143I	GATC	9 cut(s)	19, 33, 157, 763, 1044, 1089, 1235, 1411, 1593
BspACI	CCGC	7 cut(s)	57, 60, 219, 1350, 1398, 1556, 1756
BspANI	GGCC	3 cut(s)	517, 866, 1449
BspCNI	CTCAG	5 cut(s)	144, 469, 741, 967, 1690
BspFNI	CGCG	2 cut(s)	219, 1684
BspHI	TCATGA	2 cut(s)	780, 1168
BspLI	GGNNCC	4 cut(s)	21, 452, 967, 1015
BspOI	GCTAGC	1 cut(s)	182
BspPI	GGATC	6 cut(s)	14, 27, 152, 771, 1243, 1406
BspT104I	TTCGAA	1 cut(s)	828
BspT107I	GGYRCC	2 cut(s)	450, 1013
BspTNI	GGTCTC	1 cut(s)	1070
BsrFI	RCCGGY	1 cut(s)	1016
BsrGI	TGTACA	3 cut(s)	624, 820, 1731
BsrI	ACTGG	3 cut(s)	135, 518, 1450
BssAI	RCCGGY	1 cut(s)	1016
BssMI	GATC	9 cut(s)	19, 33, 157, 763, 1044, 1089, 1235, 1411, 1593
BssNAI	GTATAC	1 cut(s)	76
BssNI	GRCGYC	2 cut(s)	204, 451
BssT1I	CCWWGG	6 cut(s)	580, 994, 1160, 1551, 1656, 1669
Bst1107I	GTATAC	1 cut(s)	76
Bst2UI	CCWGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
Bst4CI	ACNGT	9 cut(s)	28, 52, 80, 412, 529, 825, 1225, 1373, 1736
Bst6I	CTCTTC	3 cut(s)	768, 1068, 1146
BstACI	GRCGYC	2 cut(s)	204, 451
BstAPI	GCANNNNNTGC	1 cut(s)	1391
BstAUI	TGTACA	3 cut(s)	624, 820, 1731
BstBI	TTCGAA	1 cut(s)	828
BstC8I	GCNNGC	7 cut(s)	7, 11, 180, 424, 864, 1396, 1716
BstDEI	CTNAG	5 cut(s)	152, 456, 728, 975, 1698
BstEII	GGTNACC	1 cut(s)	575
BstF5I	GGATG	4 cut(s)	76, 279, 600, 1204
BstFNI	CGCG	2 cut(s)	219, 1684
BstH2I	RGCGCY	2 cut(s)	454, 1002
BstHHI	GCGC	3 cut(s)	219, 453, 1001
BstKTI	GATC	9 cut(s)	22, 36, 160, 766, 1047, 1092, 1238, 1414, 1596
BstMAI	GTCTC	2 cut(s)	1070, 1691
BstMBI	GATC	9 cut(s)	19, 33, 157, 763, 1044, 1089, 1235, 1411, 1593
BstMWI	GCNNNNNNNGC	8 cut(s)	1007, 1208, 1358, 1391, 1547, 1637, 1649, 1658
BstNI	CCWGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
BstNSI	RCATGY	3 cut(s)	1531, 1718, 1732
BstPI	GGTNACC	1 cut(s)	575
BstSCI	CCNGG	6 cut(s)	116, 255, 330, 564, 1444, 1474
BstSFI	CTRYAG	2 cut(s)	921, 1744
BstUI	CGCG	2 cut(s)	219, 1684
BstV1I	GCAGC	6 cut(s)	846, 956, 1168, 1550, 1627, 1639
BstV2I	GAAGAC	2 cut(s)	215, 883
BstX2I	RGATCY	5 cut(s)	19, 157, 763, 1044, 1089
BstYI	RGATCY	5 cut(s)	19, 157, 763, 1044, 1089
BstZ17I	GTATAC	1 cut(s)	76
Bsu36I	CCTNAGG	1 cut(s)	456
BsuI	GTATCC	1 cut(s)	572
BsuRI	GGCC	3 cut(s)	517, 866, 1449
BtsCI	GGATG	4 cut(s)	76, 279, 600, 1204
BtsI	GCAGTG	1 cut(s)	87
BtsIMutI	CAGTG	5 cut(s)	87, 142, 317, 947, 1378
Cac8I	GCNNGC	7 cut(s)	7, 11, 180, 424, 864, 1396, 1716
CciI	TCATGA	2 cut(s)	780, 1168
CfoI	GCGC	3 cut(s)	219, 453, 1001
Cfr10I	RCCGGY	1 cut(s)	1016
Cfr13I	GGNCC	1 cut(s)	100
CseI	GACGC	2 cut(s)	212, 1673
Csp6I	GTAC	7 cut(s)	625, 757, 821, 1014, 1347, 1423, 1732
CspAI	ACCGGT	1 cut(s)	1016
CviAII	CATG	6 cut(s)	781, 1169, 1175, 1528, 1715, 1729
CviQI	GTAC	7 cut(s)	625, 757, 821, 1014, 1347, 1423, 1732
DdeI	CTNAG	5 cut(s)	152, 456, 728, 975, 1698
DinI	GGCGCC	1 cut(s)	452
DpnI	GATC	9 cut(s)	21, 35, 159, 765, 1046, 1091, 1237, 1413, 1595
DpnII	GATC	9 cut(s)	19, 33, 157, 763, 1044, 1089, 1235, 1411, 1593
EaeI	YGGCCR	2 cut(s)	864, 1447
Eam1104I	CTCTTC	3 cut(s)	768, 1068, 1146
EarI	CTCTTC	3 cut(s)	768, 1068, 1146
Eco130I	CCWWGG	6 cut(s)	580, 994, 1160, 1551, 1656, 1669
Eco24I	GRGCYC	1 cut(s)	1168
Eco31I	GGTCTC	1 cut(s)	1070
Eco47I	GGWCC	1 cut(s)	100
Eco57I	CTGAAG	3 cut(s)	430, 971, 1374
Eco81I	CCTNAGG	1 cut(s)	456
Eco88I	CYCGRG	1 cut(s)	497
Eco91I	GGTNACC	1 cut(s)	575
EcoO65I	GGTNACC	1 cut(s)	575
EcoRI	GAATTC	1 cut(s)	600
EcoRII	CCWGG	6 cut(s)	116, 255, 330, 564, 1444, 1474
EcoT14I	CCWWGG	6 cut(s)	580, 994, 1160, 1551, 1656, 1669
EcoT22I	ATGCAT	1 cut(s)	1465
EcoT38I	GRGCYC	1 cut(s)	1168
EgeI	GGCGCC	1 cut(s)	452
EheI	GGCGCC	1 cut(s)	452
ErhI	CCWWGG	6 cut(s)	580, 994, 1160, 1551, 1656, 1669
Esp3I	CGTCTC	1 cut(s)	1691
FaeI	CATG	6 cut(s)	784, 1172, 1178, 1531, 1718, 1732
FalI	AAGNNNNNCTT	2 cut(s)	1125, 1157
FaqI	GGGAC	6 cut(s)	215, 281, 582, 697, 925, 1722
FatI	CATG	6 cut(s)	780, 1168, 1174, 1527, 1714, 1728
FauI	CCCGC	1 cut(s)	1549
FauNDI	CATATG	1 cut(s)	1663
FblI	GTMKAC	3 cut(s)	75, 399, 1743
Fnu4HI	GCNGC	8 cut(s)	58, 61, 860, 945, 1157, 1539, 1641, 1653
FokI	GGATG	4 cut(s)	83, 266, 607, 1211
FriOI	GRGCYC	1 cut(s)	1168
Fsp4HI	GCNGC	8 cut(s)	58, 61, 860, 945, 1157, 1539, 1641, 1653
FspBI	CTAG	1 cut(s)	179
GlaI	GCGC	3 cut(s)	218, 452, 1000
GluI	GCNGC	8 cut(s)	58, 61, 860, 945, 1157, 1539, 1641, 1653
GsuI	CTGGAG	4 cut(s)	239, 314, 1023, 1524
HaeII	RGCGCY	2 cut(s)	454, 1002
HaeIII	GGCC	3 cut(s)	517, 866, 1449
HapII	CCGG	2 cut(s)	233, 1017
HgaI	GACGC	2 cut(s)	212, 1673
HhaI	GCGC	3 cut(s)	219, 453, 1001
Hin1I	GRCGYC	2 cut(s)	204, 451
Hin1II	CATG	6 cut(s)	784, 1172, 1178, 1531, 1718, 1732
Hin6I	GCGC	3 cut(s)	217, 451, 999
HinP1I	GCGC	3 cut(s)	217, 451, 999
HindIII	AAGCTT	1 cut(s)	1359
HinfI	GANTC	4 cut(s)	726, 1103, 1136, 1702
HpaII	CCGG	2 cut(s)	233, 1017
HphI	GGTGA	7 cut(s)	92, 103, 428, 566, 587, 818, 1282
Hpy166II	GTNNAC	6 cut(s)	76, 400, 557, 627, 1519, 1744
Hpy188I	TCNGA	9 cut(s)	284, 408, 437, 731, 976, 1342, 1546, 1570, 1619
Hpy188III	TCNNGA	8 cut(s)	497, 781, 1040, 1048, 1169, 1503, 1520, 1697
Hpy8I	GTNNAC	6 cut(s)	76, 400, 557, 627, 1519, 1744
Hpy99I	CGWCG	1 cut(s)	56
HpyAV	CCTTC	2 cut(s)	478, 1300
HpyCH4III	ACNGT	9 cut(s)	28, 52, 80, 412, 529, 825, 1225, 1373, 1736
HpyCH4IV	ACGT	1 cut(s)	559
HpyCH4V	TGCA	7 cut(s)	304, 839, 932, 1385, 1463, 1583, 1728
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1007, 1208, 1358, 1391, 1547, 1637, 1649, 1658
HpyF3I	CTNAG	5 cut(s)	152, 456, 728, 975, 1698
HpySE526I	ACGT	1 cut(s)	559
Hsp92I	GRCGYC	2 cut(s)	204, 451
Hsp92II	CATG	6 cut(s)	784, 1172, 1178, 1531, 1718, 1732
HspAI	GCGC	3 cut(s)	217, 451, 999
KasI	GGCGCC	1 cut(s)	450
KpnI	GGTACC	1 cut(s)	1017
Kzo9I	GATC	9 cut(s)	19, 33, 157, 763, 1044, 1089, 1235, 1411, 1593
LmnI	GCTCC	2 cut(s)	393, 1505
Lsp1109I	GCAGC	6 cut(s)	846, 956, 1168, 1550, 1627, 1639
LweI	GCATC	4 cut(s)	450, 887, 926, 1189
MaeI	CTAG	1 cut(s)	179
MaeII	ACGT	1 cut(s)	559
MaeIII	GTNAC	3 cut(s)	544, 575, 667
MalI	GATC	9 cut(s)	21, 35, 159, 765, 1046, 1091, 1237, 1413, 1595
MboI	GATC	9 cut(s)	19, 33, 157, 763, 1044, 1089, 1235, 1411, 1593
MfeI	CAATTG	2 cut(s)	393, 672
MflI	RGATCY	5 cut(s)	19, 157, 763, 1044, 1089
MhlI	GDGCHC	1 cut(s)	1168
MlsI	TGGCCA	2 cut(s)	866, 1449
MluCI	AATT	8 cut(s)	383, 393, 431, 600, 672, 1212, 1386, 1576
MluI	ACGCGT	1 cut(s)	1682
MluNI	TGGCCA	2 cut(s)	866, 1449
Mly113I	GGCGCC	1 cut(s)	451
MlyI	GAGTC	1 cut(s)	720
MmeI	TCCRAC	5 cut(s)	307, 431, 1228, 1347, 1479
Mox20I	TGGCCA	2 cut(s)	866, 1449
Mph1103I	ATGCAT	1 cut(s)	1465
MroXI	GAANNNNTTC	1 cut(s)	1624
MscI	TGGCCA	2 cut(s)	866, 1449
MseI	TTAA	4 cut(s)	30, 956, 1152, 1215
MslI	CAYNNNNRTG	1 cut(s)	1173
Msp20I	TGGCCA	2 cut(s)	866, 1449
MspA1I	CMGCKG	2 cut(s)	1352, 1398
MspI	CCGG	2 cut(s)	233, 1017
MspR9I	CCNGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
MunI	CAATTG	2 cut(s)	393, 672
MvaI	CCWGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
MvnI	CGCG	2 cut(s)	219, 1684
MwoI	GCNNNNNNNGC	8 cut(s)	1007, 1208, 1358, 1391, 1547, 1637, 1649, 1658
NarI	GGCGCC	1 cut(s)	451
NdeI	CATATG	1 cut(s)	1663
NdeII	GATC	9 cut(s)	19, 33, 157, 763, 1044, 1089, 1235, 1411, 1593
NheI	GCTAGC	1 cut(s)	178
NlaIII	CATG	6 cut(s)	784, 1172, 1178, 1531, 1718, 1732
NlaIV	GGNNCC	4 cut(s)	21, 452, 967, 1015
NmeAIII	GCCGAG	1 cut(s)	1744
NmuCI	GTSAC	2 cut(s)	544, 575
NsiI	ATGCAT	1 cut(s)	1465
NspI	RCATGY	3 cut(s)	1531, 1718, 1732
NspV	TTCGAA	1 cut(s)	828
PaeI	GCATGC	1 cut(s)	1718
PaeR7I	CTCGAG	1 cut(s)	497
PagI	TCATGA	2 cut(s)	780, 1168
PcsI	WCGNNNNNNNCGW	1 cut(s)	60
PdmI	GAANNNNTTC	1 cut(s)	1624
PfeI	GAWTC	3 cut(s)	1103, 1136, 1702
PfoI	TCCNGGA	3 cut(s)	255, 330, 1474
PinAI	ACCGGT	1 cut(s)	1016
PkrI	GCNGC	8 cut(s)	59, 62, 861, 946, 1158, 1540, 1642, 1654
PleI	GAGTC	1 cut(s)	720
PluTI	GGCGCC	1 cut(s)	454
PpsI	GAGTC	1 cut(s)	720
Psp6I	CCWGG	6 cut(s)	116, 255, 330, 564, 1444, 1474
PspEI	GGTNACC	1 cut(s)	575
PspGI	CCWGG	6 cut(s)	116, 255, 330, 564, 1444, 1474
PspN4I	GGNNCC	4 cut(s)	21, 452, 967, 1015
PspPI	GGNCC	1 cut(s)	100
PsrI	GAACNNNNNNTAC	2 cut(s)	513, 545
PsuI	RGATCY	5 cut(s)	19, 157, 763, 1044, 1089
RsaI	GTAC	7 cut(s)	626, 758, 822, 1015, 1348, 1424, 1733
RsaNI	GTAC	7 cut(s)	625, 757, 821, 1014, 1347, 1423, 1732
RseI	CAYNNNNRTG	1 cut(s)	1173
SaqAI	TTAA	4 cut(s)	30, 956, 1152, 1215
SatI	GCNGC	8 cut(s)	58, 61, 860, 945, 1157, 1539, 1641, 1653
Sau3AI	GATC	9 cut(s)	19, 33, 157, 763, 1044, 1089, 1235, 1411, 1593
Sau96I	GGNCC	1 cut(s)	100
SchI	GAGTC	1 cut(s)	720
ScrFI	CCNGG	6 cut(s)	118, 257, 332, 566, 1446, 1476
SduI	GDGCHC	1 cut(s)	1168
SfaNI	GCATC	4 cut(s)	450, 887, 926, 1189
SfcI	CTRYAG	2 cut(s)	921, 1744
SfoI	GGCGCC	1 cut(s)	452
Sfr274I	CTCGAG	1 cut(s)	497
SfuI	TTCGAA	1 cut(s)	828
SinI	GGWCC	1 cut(s)	100
SlaI	CTCGAG	1 cut(s)	497
SmiMI	CAYNNNNRTG	1 cut(s)	1173
SmlI	CTYRAG	2 cut(s)	497, 1040
SmoI	CTYRAG	2 cut(s)	497, 1040
SphI	GCATGC	1 cut(s)	1718
Sse9I	AATT	8 cut(s)	383, 393, 431, 600, 672, 1212, 1386, 1576
SsiI	CCGC	7 cut(s)	57, 60, 219, 1350, 1398, 1556, 1756
SspDI	GGCGCC	1 cut(s)	450
SspMI	CTAG	1 cut(s)	179
StyD4I	CCNGG	6 cut(s)	116, 255, 330, 564, 1444, 1474
StyI	CCWWGG	6 cut(s)	580, 994, 1160, 1551, 1656, 1669
TaaI	ACNGT	9 cut(s)	28, 52, 80, 412, 529, 825, 1225, 1373, 1736
TaiI	ACGT	1 cut(s)	562
TaqI	TCGA	6 cut(s)	36, 378, 498, 828, 1049, 1610
TasI	AATT	8 cut(s)	383, 393, 431, 600, 672, 1212, 1386, 1576
TatI	WGTACW	4 cut(s)	624, 820, 1422, 1731
TauI	GCSGC	2 cut(s)	60, 63
TfiI	GAWTC	3 cut(s)	1103, 1136, 1702
Tru1I	TTAA	4 cut(s)	30, 956, 1152, 1215
Tru9I	TTAA	4 cut(s)	30, 956, 1152, 1215
TscAI	CASTG	5 cut(s)	94, 142, 324, 954, 1378
TseFI	GTSAC	2 cut(s)	544, 575
TseI	GCWGC	6 cut(s)	859, 944, 1156, 1538, 1640, 1652
Tsp45I	GTSAC	2 cut(s)	544, 575
TspDTI	ATGAA	2 cut(s)	287, 769
TspGWI	ACGGA	4 cut(s)	32, 118, 338, 1502
TspRI	CASTG	5 cut(s)	94, 142, 324, 954, 1378
VpaK11BI	GGWCC	1 cut(s)	100
XapI	RAATTY	3 cut(s)	431, 600, 1576
XceI	RCATGY	3 cut(s)	1531, 1718, 1732
XhoI	CTCGAG	1 cut(s)	497
XmiI	GTMKAC	3 cut(s)	75, 399, 1743
XmnI	GAANNNNTTC	1 cut(s)	1624
XspI	CTAG	1 cut(s)	179
Zsp2I	ATGCAT	1 cut(s)	1465

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.