Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0005972

Physical Location & Seq

Genomic Coordinates Forward (+)

4922027 .. 4927974

Gene Structure 5948 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG064200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGGAGAGCACACACAAGGAGGTGAGGGATGGTGTTTCTGTGCTTGACCTGGACCCCACAGCCGCCGTCGGTGGCGGCGTTGAGGACGTCTACGGTGAGGATGCTGCCACCGAGGACCAACTCGTCACTCCCTGGACTTTCTCTGTAGCCAGTGGGTACTCTCTGCTGAGAGATCCTCAATACAACAAAGGGCTAGCATTTACCGAGAAAGAGAGGGATGCTCATTACTTGCGCGGTCTTCTGCCTCCGGCTACTTCCACTCAACAACTTCAGGAGAAGAAGCTGATGCACAATATCAGACAATACCAAGTGCCGCTGCAAAAATATATGGCACTGACCGAACTCCAGGAGAGGAATGAGAGGCTGTTCTACAAACTTCTCATTGATAATGTCGAGGAATTGCTCCCGATTGTATATACTCCAACTGTTGGTGAGGCTTGCCAGAAATATGGAAGCATTTTCAGACGCCCTCAGGGTCTTTATATAAGTCTGAAAGAGAAAGGTAGAATTCTCGAGGTATTGAAGAACTGGCCTGAAAGGACTATTCAAGTTATTGTTGTCACTGATGGTGAGCGTATATTGGGACTTGGTGACCTTGGTTGTCAGGGGATGGGAATTCCTGTAGGGAAGTTGGCTTTGTACACAGCTCTTGGAGGAGTTCGTCCCTCAGCATGTTTGCCTATAACCATTGATGTTGGGACAAATAATGAGCAGTTGCTGAAGGATGAGTTCTACATTGGGCTCAGACAAAAGAGAGCAACTGGAAAGGAATATGCTGAGCTTTTACATGAGTTCATGGGTGCTGTGAAGCAGAACTATGGCGAAAAAGTTGTTGTACAGTTTGAAGATTTCGCTAACCACAATGCCTTTGAGCTGCTGGCAAAATATCGAAATACTCATCTCGTCTTCAATGATGACATACAGGGGACTGCTGCTGTAGTTCTTGCTGGAGTTGTGGCGGCACTAAAGTTGATAGGTGGTACCCTGTCTGAGCACAAGTTTTTGTTCCTCGGTGCTGGGGAAGCTGGGACAGGTATAGCAGAGCTAATAGCTCTTGAGATCTCAAAAAAGACAAATGTTCCTGTGGAAGAGACGCGCAAAAAGATCTGGCTTGTTGATTCAAAGGGCCTAATTGTTAGCTCTCGTAAAGAATCTCTTCAAAATTTTAAGAAGCCTTGGGCTCATGAACATGAACCCGTTAAGGATCTCATTGATGCTGTCAAGGCAATTAAACCAACAGTTCTTATTGGAACATCTGGTATTGGAAAAACATTCACAAAAGAAGTGATTGAGGCACTAGCTTCCTTCAATGAGAAACCTCTCATTTTGGCTCTTTCCAACCCAACATCACAGTCAGAATGTACAGCTGAAGAAGCTTATACTTGGACTAAGGGTCGTGCAATTTTTGCTAGTGGAAGTCCTTTCGATCCTTTTGAATATGATGGCAAAGTTTATATTCCTGGCCAGTCCAACAATGCTTACATTTTCCCTGGATTTGGTCTTGGCTTGGTCATCTCTGGAGCAATTCGGGTGCATGATGATATGCTTCTAGCGGCTTGTAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006090 GO:0006108 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0055114 GO:0065003 GO:0071704 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

521

Amino Acids

57.47

Weight (kDa)

5.82

Isoelectric Point (pI)

37.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	116 - 297	6.9e-79	Malic enzyme, N-terminal domain
Malic_M	PF03949	307 - 520	1.2e-82	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19900 AT2G19900
fragaria_vesca	FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000
malus_domestica	MD02G1064800.v1.1 MD15G1195500.v1.1
prunus_persica	Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1
pyrus_communis	pycom02g05090 pycom15g17470
rosa_chinensis	RchiOBHm_Chr2g0091741
rosa_laevigata	RLG00000016248
rosa_roxburghii	Rroxscaffold_2G00149780
rosa_rugosa	Rorug02G0020300
rosa_samantha	Rh2BG064200 Rh2CG065800 Rh2DG064300
rosa_wichuraiana	Rw2G005550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	122
AatII	GACGTC	1 cut(s)	90
Acc65I	GGTACC	1 cut(s)	980
AccB1I	GGYRCC	1 cut(s)	980
AccB7I	CCANNNNNTGG	1 cut(s)	428
AccI	GTMKAC	1 cut(s)	90
AccII	CGCG	2 cut(s)	234, 1096
AciI	CCGC	6 cut(s)	63, 75, 234, 314, 959, 1553
AclWI	GGATC	3 cut(s)	167, 1212, 1421
AcoI	YGGCCR	1 cut(s)	1462
AcsI	RAATTY	3 cut(s)	507, 615, 1162
AcuI	CTGAAG	3 cut(s)	254, 740, 1389
AcyI	GRCGYC	2 cut(s)	87, 466
AfaI	GTAC	5 cut(s)	158, 641, 837, 982, 1363
AfiI	CCNNNNNNNGG	2 cut(s)	428, 1496
AgsI	TTSAA	8 cut(s)	523, 548, 845, 910, 1122, 1160, 1309, 1436
AhdI	GACNNNNNGTC	1 cut(s)	1392
AjnI	CCWGG	5 cut(s)	48, 131, 345, 1459, 1489
Alw21I	GWGCWC	2 cut(s)	11, 996
Alw26I	GTCTC	1 cut(s)	1085
AlwI	GGATC	3 cut(s)	167, 1212, 1421
AlwNI	CAGNNNCTG	1 cut(s)	718
Ama87I	CYCGRG	1 cut(s)	512
AoxI	GGCC	3 cut(s)	530, 1126, 1462
ApeKI	GCWGC	4 cut(s)	104, 316, 874, 932
ApoI	RAATTY	3 cut(s)	507, 615, 1162
Asp700I	GAANNNNTTC	1 cut(s)	1155
Asp718I	GGTACC	1 cut(s)	980
AspLEI	GCGC	2 cut(s)	234, 1098
AspS9I	GGNCC	3 cut(s)	52, 115, 1126
AsuHPI	GGTGA	5 cut(s)	34, 107, 443, 581, 602
AsuNHI	GCTAGC	1 cut(s)	193
AvaI	CYCGRG	1 cut(s)	512
AvaII	GGWCC	2 cut(s)	52, 115
AxyI	CCTNAGG	1 cut(s)	471
BalI	TGGCCA	1 cut(s)	1464
BanI	GGYRCC	1 cut(s)	980
BanII	GRGCYC	2 cut(s)	744, 1183
BbsI	GAAGAC	2 cut(s)	230, 898
Bbv12I	GWGCWC	2 cut(s)	11, 996
BbvCI	CCTCAGC	1 cut(s)	667
BbvI	GCAGC	4 cut(s)	91, 303, 861, 919
BccI	CCATC	4 cut(s)	23, 560, 604, 1436
BceAI	ACGGC	1 cut(s)	50
BciT130I	CCWGG	5 cut(s)	50, 133, 347, 1461, 1491
BcoDI	GTCTC	1 cut(s)	1085
BfaI	CTAG	4 cut(s)	194, 1298, 1410, 1550
BfmI	CTRYAG	3 cut(s)	144, 621, 936
BglII	AGATCT	2 cut(s)	1059, 1104
BisI	GCNGC	9 cut(s)	63, 76, 105, 314, 317, 875, 933, 960, 1554
BlpI	GCTNAGC	1 cut(s)	777
BlsI	GCNGC	9 cut(s)	64, 77, 106, 315, 318, 876, 934, 961, 1555
Bme1390I	CCNGG	5 cut(s)	50, 133, 347, 1461, 1491
Bme18I	GGWCC	2 cut(s)	52, 115
BmeRI	GACNNNNNGTC	1 cut(s)	1392
BmeT110I	CYCGRG	1 cut(s)	512
BmgT120I	GGNCC	3 cut(s)	52, 115, 1126
BmiI	GGNNCC	2 cut(s)	54, 982
BmrFI	CCNGG	5 cut(s)	50, 133, 347, 1461, 1491
BmsI	GCATC	4 cut(s)	91, 208, 276, 1204
BmtI	GCTAGC	1 cut(s)	197
BpiI	GAAGAC	2 cut(s)	230, 898
BplI	GAGNNNNNCTC	4 cut(s)	160, 192, 205, 237
BpmI	CTGGAG	3 cut(s)	329, 969, 1539
Bpu10I	CCTNAGC	1 cut(s)	667
Bpu1102I	GCTNAGC	1 cut(s)	777
BpuEI	CTTGAG	1 cut(s)	1076
BsaHI	GRCGYC	2 cut(s)	87, 466
BsaJI	CCNNGG	6 cut(s)	111, 131, 595, 1009, 1175, 1489
Bsc4I	CCNNNNNNNGG	2 cut(s)	428, 1496
Bse1I	ACTGG	4 cut(s)	150, 533, 766, 1465
Bse21I	CCTNAGG	1 cut(s)	471
BseBI	CCWGG	5 cut(s)	50, 133, 347, 1461, 1491
BseDI	CCNNGG	6 cut(s)	111, 131, 595, 1009, 1175, 1489
BseGI	GGATG	5 cut(s)	34, 106, 223, 615, 730
BseLI	CCNNNNNNNGG	2 cut(s)	428, 1496
BseMII	CTCAG	6 cut(s)	158, 485, 681, 757, 768, 981
BseNI	ACTGG	4 cut(s)	150, 533, 766, 1465
BseRI	GAGGAG	1 cut(s)	669
BseXI	GCAGC	4 cut(s)	91, 303, 861, 919
BseYI	CCCAGC	2 cut(s)	1016, 1025
Bsh1236I	CGCG	2 cut(s)	234, 1096
BshFI	GGCC	3 cut(s)	532, 1128, 1464
BshNI	GGYRCC	1 cut(s)	980
BsiHKAI	GWGCWC	2 cut(s)	11, 996
BsiHKCI	CYCGRG	1 cut(s)	512
BsiSI	CCGG	1 cut(s)	248
BslFI	GGGAC	5 cut(s)	597, 648, 712, 940, 1042
BslI	CCNNNNNNNGG	2 cut(s)	428, 1496
BsmAI	GTCTC	1 cut(s)	1085
BsmBI	CGTCTC	1 cut(s)	1085
BsmFI	GGGAC	5 cut(s)	597, 648, 712, 940, 1042
BsnI	GGCC	3 cut(s)	532, 1128, 1464
BsoBI	CYCGRG	1 cut(s)	512
Bsp1286I	GDGCHC	4 cut(s)	11, 744, 996, 1183
Bsp1407I	TGTACA	3 cut(s)	639, 835, 1361
Bsp143I	GATC	5 cut(s)	172, 1059, 1104, 1204, 1426
Bsp1720I	GCTNAGC	1 cut(s)	777
BspACI	CCGC	6 cut(s)	63, 75, 234, 314, 959, 1553
BspANI	GGCC	3 cut(s)	532, 1128, 1464
BspCNI	CTCAG	6 cut(s)	159, 484, 680, 756, 769, 982
BspFNI	CGCG	2 cut(s)	234, 1096
BspHI	TCATGA	1 cut(s)	1183
BspLI	GGNNCC	2 cut(s)	54, 982
BspOI	GCTAGC	1 cut(s)	197
BspPI	GGATC	3 cut(s)	167, 1212, 1421
BspT107I	GGYRCC	1 cut(s)	980
BsrGI	TGTACA	3 cut(s)	639, 835, 1361
BsrI	ACTGG	4 cut(s)	150, 533, 766, 1465
BssECI	CCNNGG	6 cut(s)	111, 131, 595, 1009, 1175, 1489
BssMI	GATC	5 cut(s)	172, 1059, 1104, 1204, 1426
BssNI	GRCGYC	2 cut(s)	87, 466
BssT1I	CCWWGG	2 cut(s)	595, 1175
Bst2UI	CCWGG	5 cut(s)	50, 133, 347, 1461, 1491
Bst4CI	ACNGT	5 cut(s)	95, 427, 840, 1240, 1353
Bst6I	CTCTTC	2 cut(s)	1083, 1161
BstACI	GRCGYC	2 cut(s)	87, 466
BstAPI	GCANNNNNTGC	1 cut(s)	1406
BstAUI	TGTACA	3 cut(s)	639, 835, 1361
BstC8I	GCNNGC	3 cut(s)	195, 439, 879
BstDEI	CTNAG	7 cut(s)	167, 471, 667, 743, 777, 990, 1389
BstEII	GGTNACC	1 cut(s)	590
BstF5I	GGATG	5 cut(s)	34, 106, 223, 615, 730
BstFNI	CGCG	2 cut(s)	234, 1096
BstHHI	GCGC	2 cut(s)	234, 1098
BstKTI	GATC	5 cut(s)	175, 1062, 1107, 1207, 1429
BstMAI	GTCTC	1 cut(s)	1085
BstMBI	GATC	5 cut(s)	172, 1059, 1104, 1204, 1426
BstMWI	GCNNNNNNNGC	4 cut(s)	1022, 1223, 1373, 1406
BstNI	CCWGG	5 cut(s)	50, 133, 347, 1461, 1491
BstNSI	RCATGY	1 cut(s)	675
BstPI	GGTNACC	1 cut(s)	590
BstSCI	CCNGG	5 cut(s)	48, 131, 345, 1459, 1489
BstSFI	CTRYAG	3 cut(s)	144, 621, 936
BstUI	CGCG	2 cut(s)	234, 1096
BstV1I	GCAGC	4 cut(s)	91, 303, 861, 919
BstV2I	GAAGAC	2 cut(s)	230, 898
BstX2I	RGATCY	4 cut(s)	172, 1059, 1104, 1204
BstXI	CCANNNNNNTGG	1 cut(s)	449
BstYI	RGATCY	4 cut(s)	172, 1059, 1104, 1204
Bsu36I	CCTNAGG	1 cut(s)	471
BsuRI	GGCC	3 cut(s)	532, 1128, 1464
BtsCI	GGATG	5 cut(s)	34, 106, 223, 615, 730
BtsIMutI	CAGTG	3 cut(s)	157, 332, 561
Cac8I	GCNNGC	3 cut(s)	195, 439, 879
CaiI	CAGNNNCTG	1 cut(s)	718
CciI	TCATGA	1 cut(s)	1183
CfoI	GCGC	2 cut(s)	234, 1098
Cfr13I	GGNCC	3 cut(s)	52, 115, 1126
CseI	GACGC	2 cut(s)	474, 1102
Csp6I	GTAC	5 cut(s)	157, 640, 836, 981, 1362
CviAII	CATG	6 cut(s)	672, 788, 796, 1184, 1190, 1535
CviQI	GTAC	5 cut(s)	157, 640, 836, 981, 1362
DdeI	CTNAG	7 cut(s)	167, 471, 667, 743, 777, 990, 1389
DpnI	GATC	5 cut(s)	174, 1061, 1106, 1206, 1428
DpnII	GATC	5 cut(s)	172, 1059, 1104, 1204, 1426
DrdI	GACNNNNNNGTC	1 cut(s)	122
DriI	GACNNNNNGTC	1 cut(s)	1392
DseDI	GACNNNNNNGTC	1 cut(s)	122
EaeI	YGGCCR	1 cut(s)	1462
Eam1104I	CTCTTC	2 cut(s)	1083, 1161
Eam1105I	GACNNNNNGTC	1 cut(s)	1392
EarI	CTCTTC	2 cut(s)	1083, 1161
Eco130I	CCWWGG	2 cut(s)	595, 1175
Eco24I	GRGCYC	2 cut(s)	744, 1183
Eco47I	GGWCC	2 cut(s)	52, 115
Eco57I	CTGAAG	3 cut(s)	254, 740, 1389
Eco81I	CCTNAGG	1 cut(s)	471
Eco88I	CYCGRG	1 cut(s)	512
Eco91I	GGTNACC	1 cut(s)	590
EcoO109I	RGGNCCY	1 cut(s)	1126
EcoO65I	GGTNACC	1 cut(s)	590
EcoRI	GAATTC	2 cut(s)	507, 615
EcoRII	CCWGG	5 cut(s)	48, 131, 345, 1459, 1489
EcoT14I	CCWWGG	2 cut(s)	595, 1175
EcoT38I	GRGCYC	2 cut(s)	744, 1183
ErhI	CCWWGG	2 cut(s)	595, 1175
Esp3I	CGTCTC	1 cut(s)	1085
FaeI	CATG	6 cut(s)	675, 791, 799, 1187, 1193, 1538
FalI	AAGNNNNNCTT	2 cut(s)	1140, 1172
FaqI	GGGAC	5 cut(s)	597, 648, 712, 940, 1042
FatI	CATG	6 cut(s)	671, 787, 795, 1183, 1189, 1534
FblI	GTMKAC	1 cut(s)	90
Fnu4HI	GCNGC	9 cut(s)	63, 76, 105, 314, 317, 875, 933, 960, 1554
FokI	GGATG	5 cut(s)	41, 113, 230, 622, 737
FriOI	GRGCYC	2 cut(s)	744, 1183
Fsp4HI	GCNGC	9 cut(s)	63, 76, 105, 314, 317, 875, 933, 960, 1554
FspBI	CTAG	4 cut(s)	194, 1298, 1410, 1550
GlaI	GCGC	2 cut(s)	233, 1097
GluI	GCNGC	9 cut(s)	63, 76, 105, 314, 317, 875, 933, 960, 1554
GsaI	CCCAGC	2 cut(s)	1020, 1029
GsuI	CTGGAG	3 cut(s)	329, 969, 1539
HaeIII	GGCC	3 cut(s)	532, 1128, 1464
HapII	CCGG	1 cut(s)	248
HgaI	GACGC	2 cut(s)	474, 1102
HhaI	GCGC	2 cut(s)	234, 1098
Hin1I	GRCGYC	2 cut(s)	87, 466
Hin1II	CATG	6 cut(s)	675, 791, 799, 1187, 1193, 1538
Hin6I	GCGC	2 cut(s)	232, 1096
HinP1I	GCGC	2 cut(s)	232, 1096
HindIII	AAGCTT	1 cut(s)	1374
HinfI	GANTC	2 cut(s)	1118, 1151
HpaII	CCGG	1 cut(s)	248
HphI	GGTGA	5 cut(s)	34, 107, 443, 581, 602
Hpy166II	GTNNAC	2 cut(s)	91, 642
Hpy188I	TCNGA	6 cut(s)	299, 464, 492, 746, 991, 1357
Hpy188III	TCNNGA	6 cut(s)	272, 406, 512, 1055, 1184, 1518
Hpy8I	GTNNAC	2 cut(s)	91, 642
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	2 cut(s)	715, 1315
HpyCH4III	ACNGT	5 cut(s)	95, 427, 840, 1240, 1353
HpyCH4IV	ACGT	1 cut(s)	87
HpyCH4V	TGCA	4 cut(s)	289, 319, 1400, 1534
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1022, 1223, 1373, 1406
HpyF3I	CTNAG	7 cut(s)	167, 471, 667, 743, 777, 990, 1389
HpySE526I	ACGT	1 cut(s)	87
Hsp92I	GRCGYC	2 cut(s)	87, 466
Hsp92II	CATG	6 cut(s)	675, 791, 799, 1187, 1193, 1538
HspAI	GCGC	2 cut(s)	232, 1096
KpnI	GGTACC	1 cut(s)	984
Kzo9I	GATC	5 cut(s)	172, 1059, 1104, 1204, 1426
LmnI	GCTCC	2 cut(s)	408, 1520
Lsp1109I	GCAGC	4 cut(s)	91, 303, 861, 919
LweI	GCATC	4 cut(s)	91, 208, 276, 1204
MaeI	CTAG	4 cut(s)	194, 1298, 1410, 1550
MaeII	ACGT	1 cut(s)	87
MaeIII	GTNAC	3 cut(s)	124, 559, 590
MalI	GATC	5 cut(s)	174, 1061, 1106, 1206, 1428
MboI	GATC	5 cut(s)	172, 1059, 1104, 1204, 1426
MboII	GAAGA	8 cut(s)	230, 289, 535, 857, 898, 1100, 1148, 1382
MflI	RGATCY	4 cut(s)	172, 1059, 1104, 1204
MhlI	GDGCHC	4 cut(s)	11, 744, 996, 1183
MlsI	TGGCCA	1 cut(s)	1464
MluCI	AATT	8 cut(s)	398, 507, 615, 1131, 1162, 1227, 1401, 1524
MluNI	TGGCCA	1 cut(s)	1464
MmeI	TCCRAC	3 cut(s)	446, 1362, 1494
Mox20I	TGGCCA	1 cut(s)	1464
MroXI	GAANNNNTTC	1 cut(s)	1155
MscI	TGGCCA	1 cut(s)	1464
MseI	TTAA	3 cut(s)	1167, 1200, 1230
MslI	CAYNNNNRTG	1 cut(s)	1188
Msp20I	TGGCCA	1 cut(s)	1464
MspA1I	CMGCKG	2 cut(s)	316, 1367
MspI	CCGG	1 cut(s)	248
MspR9I	CCNGG	5 cut(s)	50, 133, 347, 1461, 1491
MvaI	CCWGG	5 cut(s)	50, 133, 347, 1461, 1491
MvnI	CGCG	2 cut(s)	234, 1096
MwoI	GCNNNNNNNGC	4 cut(s)	1022, 1223, 1373, 1406
NdeII	GATC	5 cut(s)	172, 1059, 1104, 1204, 1426
NheI	GCTAGC	1 cut(s)	193
NlaIII	CATG	6 cut(s)	675, 791, 799, 1187, 1193, 1538
NlaIV	GGNNCC	2 cut(s)	54, 982
NmuCI	GTSAC	3 cut(s)	124, 559, 590
NspI	RCATGY	1 cut(s)	675
PaeR7I	CTCGAG	1 cut(s)	512
PagI	TCATGA	1 cut(s)	1183
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PdmI	GAANNNNTTC	1 cut(s)	1155
PfeI	GAWTC	2 cut(s)	1118, 1151
PflMI	CCANNNNNTGG	1 cut(s)	428
PfoI	TCCNGGA	1 cut(s)	345
PkrI	GCNGC	9 cut(s)	64, 77, 106, 315, 318, 876, 934, 961, 1555
Psp6I	CCWGG	5 cut(s)	48, 131, 345, 1459, 1489
PspEI	GGTNACC	1 cut(s)	590
PspFI	CCCAGC	2 cut(s)	1016, 1025
PspGI	CCWGG	5 cut(s)	48, 131, 345, 1459, 1489
PspN4I	GGNNCC	2 cut(s)	54, 982
PspPI	GGNCC	3 cut(s)	52, 115, 1126
PstNI	CAGNNNCTG	1 cut(s)	718
PsuI	RGATCY	4 cut(s)	172, 1059, 1104, 1204
PvuII	CAGCTG	1 cut(s)	1367
RsaI	GTAC	5 cut(s)	158, 641, 837, 982, 1363
RsaNI	GTAC	5 cut(s)	157, 640, 836, 981, 1362
RseI	CAYNNNNRTG	1 cut(s)	1188
SaqAI	TTAA	3 cut(s)	1167, 1200, 1230
SatI	GCNGC	9 cut(s)	63, 76, 105, 314, 317, 875, 933, 960, 1554
Sau3AI	GATC	5 cut(s)	172, 1059, 1104, 1204, 1426
Sau96I	GGNCC	3 cut(s)	52, 115, 1126
ScrFI	CCNGG	5 cut(s)	50, 133, 347, 1461, 1491
SduI	GDGCHC	4 cut(s)	11, 744, 996, 1183
SfaNI	GCATC	4 cut(s)	91, 208, 276, 1204
SfcI	CTRYAG	3 cut(s)	144, 621, 936
Sfr274I	CTCGAG	1 cut(s)	512
SinI	GGWCC	2 cut(s)	52, 115
SlaI	CTCGAG	1 cut(s)	512
SmiMI	CAYNNNNRTG	1 cut(s)	1188
SmlI	CTYRAG	2 cut(s)	512, 1055
SmoI	CTYRAG	2 cut(s)	512, 1055
Sse9I	AATT	8 cut(s)	398, 507, 615, 1131, 1162, 1227, 1401, 1524
SsiI	CCGC	6 cut(s)	63, 75, 234, 314, 959, 1553
SspMI	CTAG	4 cut(s)	194, 1298, 1410, 1550
StyD4I	CCNGG	5 cut(s)	48, 131, 345, 1459, 1489
StyI	CCWWGG	2 cut(s)	595, 1175
TaaI	ACNGT	5 cut(s)	95, 427, 840, 1240, 1353
TaiI	ACGT	1 cut(s)	90
TaqI	TCGA	4 cut(s)	393, 513, 889, 1425
TaqII	GACCGA	1 cut(s)	353
TasI	AATT	8 cut(s)	398, 507, 615, 1131, 1162, 1227, 1401, 1524
TatI	WGTACW	3 cut(s)	639, 835, 1361
TauI	GCSGC	5 cut(s)	65, 78, 316, 962, 1556
TfiI	GAWTC	2 cut(s)	1118, 1151
Tru1I	TTAA	3 cut(s)	1167, 1200, 1230
Tru9I	TTAA	3 cut(s)	1167, 1200, 1230
TscAI	CASTG	3 cut(s)	157, 339, 568
TseFI	GTSAC	3 cut(s)	124, 559, 590
TseI	GCWGC	4 cut(s)	104, 316, 874, 932
Tsp45I	GTSAC	3 cut(s)	124, 559, 590
TspDTI	ATGAA	3 cut(s)	784, 1200, 1206
TspRI	CASTG	3 cut(s)	157, 339, 568
Van91I	CCANNNNNTGG	1 cut(s)	428
VpaK11BI	GGWCC	2 cut(s)	52, 115
XapI	RAATTY	3 cut(s)	507, 615, 1162
XceI	RCATGY	1 cut(s)	675
XhoI	CTCGAG	1 cut(s)	512
XmiI	GTMKAC	1 cut(s)	90
XmnI	GAANNNNTTC	1 cut(s)	1155
XspI	CTAG	4 cut(s)	194, 1298, 1410, 1550
ZraI	GACGTC	1 cut(s)	88

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.