Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005972

Physical Location & Seq

Genomic Coordinates Forward (+)

15485382 .. 15490016

Gene Structure 4635 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1195500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGCGAGCAAGGTCACGGATTCTACGGTTGAGATCGACCCCAAGTCCACCGTCGGCGGCGGCGTTGAGGATGTCTATGGTGAGGACACTGCCACCGAAGACCAACTTGTCACCCCCTGGACTTACTCCGTTGCCAGTGGATATTCTTTGCTGAGAGATCCTCAATACAATAAAGGGCTTGCATTTACAGAGAAAGAGAGGGACGCTCATTACTTGCGTGGCCTTCTGCCTCCAGCAACTGCTTCTCAACAACTCCAGGAGAGGAAGTTGATGAACACTATCCGACAATATCAAGTCCCGCTGCAAAAATACATGGCGTTAACGGAGCTCCAGGAGAGGAATGAGAGGCTGTTCTACAAACTGCTCATTGATAATGTCGAGGAATTACTTCCGATTGTCTACACTCCGACTGTTGGTGAGGCTTGCCAGAAATTCGGAAGCATCTTCAGGCGCCCTCAGGGTCTATACATTAGTCTGAAAGAGAAGGGTAAAGTTCTAGAGGTATTGAAGAACTGGCCTGAAAGGACTGTTCAGGTTATTGTCGTGACTGATGGTGAACGTATATTGGGACTTGGTGACCTCGGTTGTCAGGGGATGGGAATTCCCGTAGGGAAGTTGGCTTTGTACACGGCACTTGGAGGAGTTCGCCCCTCAGCATGTTTGCCTGTGACGATTGATGTTGGGACAAACAATGAGCAGTTGCTGGAGGATGAGTTTTACATCGGACTCAGACAAAAGAGGACAAGGGGAAAGGAGTATGAGGATCTTTTACAAGAGTTCATGAGTGCCGTCAAGCAGAACTATGGCGAAAAAGTTATTGTACAGTTTGAAGATTTTGCAAACCACAATGCTTTCGAGCTGCTGTCCAGATATAGAACATCTCATCTCGTCTTCAATGATGACATACAGGGGACGGCATCTGTAGTTCTTGCAGGAGTTGTGGCAGCACTTAAATTGATAGGTGGTTCCATGTCTGAGCACAGGTTTTTGTTCCTTGGTGCCGGCGAAGCTGGTACCGGTATAGCAGAGCTAATAGCTCTTGAGATCTCAAAAAAGACAAAAGTTCCTGTGGAAGAGACCCGTAAGAAGATCTGGCTTGTTGATTCAAAGGGATTGATTGTTAGCTCTCGTAAAGATTCTCTTCAACATTTTAAGCAGCCTTGGGCTCATGAACATGAGCCTGTTAAGGATCTTCTGGATGCTGTCAAGGCAATTAAACCAACTGTTCTTATCGGATCATCTGGTGTTGGAAGAACATTTACAAAGGAAGTGATCGAAGCAGTTTCGTCCTTCAATGAGAAACCTCTTATTTTGGCTCTTTCCAACCCAACATCACAATCTGAATGTACCGCTGAAGAAGCTTATACTTACAGTGAGGGTCGCGCAATTTTTGCCAGCGGAAGTCCATTTGATCCTGTTGAGTACAATGGCAAAGTTTTCATTCCTGGCCAGTCCAACAATGCATACATTTTTCCTGGATTCGGTCTGGGTTTGGTTATCTCTGGAGCAATCCGTGTTCACGATGACATGCTTCTGGCAGCCTCGGAAGCTTTGGCGGGGCTAGTTTCTGAGGAGAATTTTGCACAGGGGCTGATTTATCCACCATTGTCAAACATCAGAAAGATTTCTGCCCAAATAGCTGCTAATGTAGCTGCAAAAGCATATGACCTTGGGGTGGCTACGCGTCTCCCTCGTCCTGAGAATCTTCTGAAGTATGCCGAGAGTTGCATGTACAGTCCCATCTACCGAAACTACCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006090 GO:0006108 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0055114 GO:0065003 GO:0071704 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

587

Amino Acids

64.69

Weight (kDa)

5.72

Isoelectric Point (pI)

41.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	111 - 292	2.7e-79	Malic enzyme, N-terminal domain
Malic_M	PF03949	302 - 555	1.4e-95	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19900 AT2G19900
fragaria_vesca	FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000
malus_domestica	MD02G1064800.v1.1 MD15G1195500.v1.1
prunus_persica	Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1
pyrus_communis	pycom02g05090 pycom15g17470
rosa_chinensis	RchiOBHm_Chr2g0091741
rosa_laevigata	RLG00000016248
rosa_roxburghii	Rroxscaffold_2G00149780
rosa_rugosa	Rorug02G0020300
rosa_samantha	Rh2BG064200 Rh2CG065800 Rh2DG064300
rosa_wichuraiana	Rw2G005550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	107
Acc65I	GGTACC	1 cut(s)	1013
AccB1I	GGYRCC	3 cut(s)	450, 998, 1013
AccI	GTMKAC	1 cut(s)	399
AccII	CGCG	2 cut(s)	1383, 1684
AciI	CCGC	7 cut(s)	57, 60, 299, 1350, 1398, 1556, 1756
AclWI	GGATC	5 cut(s)	152, 771, 1197, 1243, 1406
AcoI	YGGCCR	1 cut(s)	1447
AcsI	RAATTY	3 cut(s)	431, 600, 1576
AcuI	CTGAAG	3 cut(s)	430, 1374, 1730
AcyI	GRCGYC	1 cut(s)	451
AfaI	GTAC	6 cut(s)	626, 822, 1015, 1348, 1424, 1733
AfiI	CCNNNNNNNGG	3 cut(s)	413, 1481, 1675
AflIII	ACRYGT	1 cut(s)	1682
AgeI	ACCGGT	1 cut(s)	1016
AgsI	TTSAA	6 cut(s)	508, 830, 895, 1107, 1145, 1294
AhdI	GACNNNNNGTC	1 cut(s)	43
AjnI	CCWGG	5 cut(s)	116, 255, 330, 1444, 1474
Alw21I	GWGCWC	2 cut(s)	330, 981
Alw26I	GTCTC	2 cut(s)	1070, 1691
AlwI	GGATC	5 cut(s)	152, 771, 1197, 1243, 1406
AlwNI	CAGNNNCTG	3 cut(s)	239, 703, 1591
AoxI	GGCC	3 cut(s)	220, 515, 1447
ApeKI	GCWGC	7 cut(s)	301, 859, 944, 1156, 1538, 1640, 1652
ApoI	RAATTY	3 cut(s)	431, 600, 1576
AsiGI	ACCGGT	1 cut(s)	1016
Asp700I	GAANNNNTTC	2 cut(s)	387, 1624
Asp718I	GGTACC	1 cut(s)	1013
AspLEI	GCGC	2 cut(s)	453, 1385
AsuHPI	GGTGA	5 cut(s)	92, 103, 428, 566, 587
AxyI	CCTNAGG	1 cut(s)	456
BalI	TGGCCA	1 cut(s)	1449
BanI	GGYRCC	3 cut(s)	450, 998, 1013
BanII	GRGCYC	2 cut(s)	330, 1168
BarI	GAAGNNNNNNTAC	2 cut(s)	1243, 1275
BbsI	GAAGAC	2 cut(s)	105, 883
Bbv12I	GWGCWC	2 cut(s)	330, 981
BbvCI	CCTCAGC	1 cut(s)	652
BbvI	GCAGC	7 cut(s)	288, 846, 956, 1168, 1550, 1627, 1639
BccI	CCATC	3 cut(s)	545, 589, 1748
BceAI	ACGGC	3 cut(s)	645, 773, 930
BciT130I	CCWGG	5 cut(s)	118, 257, 332, 1446, 1476
BcoDI	GTCTC	2 cut(s)	1070, 1691
BfaI	CTAG	2 cut(s)	497, 1562
BfmI	CTRYAG	1 cut(s)	921
BfoI	RGCGCY	1 cut(s)	454
BglII	AGATCT	2 cut(s)	1044, 1089
BisI	GCNGC	9 cut(s)	58, 61, 302, 860, 945, 1157, 1539, 1641, 1653
BlsI	GCNGC	9 cut(s)	59, 62, 303, 861, 946, 1158, 1540, 1642, 1654
Bme1390I	CCNGG	5 cut(s)	118, 257, 332, 1446, 1476
BmeRI	GACNNNNNGTC	1 cut(s)	43
BmiI	GGNNCC	4 cut(s)	452, 967, 1000, 1015
BmrFI	CCNGG	5 cut(s)	118, 257, 332, 1446, 1476
BmsI	GCATC	3 cut(s)	450, 926, 1189
BpiI	GAAGAC	2 cut(s)	105, 883
BplI	GAGNNNNNCTC	4 cut(s)	145, 177, 190, 222
BpmI	CTGGAG	5 cut(s)	216, 239, 314, 725, 1524
Bpu10I	CCTNAGC	1 cut(s)	652
BpuEI	CTTGAG	1 cut(s)	1061
BsaHI	GRCGYC	1 cut(s)	451
BsaI	GGTCTC	1 cut(s)	1070
BsaJI	CCNNGG	6 cut(s)	116, 580, 994, 1160, 1542, 1669
BsaWI	WCCGGW	1 cut(s)	1016
BsaXI	ACNNNNNCTCC	4 cut(s)	251, 281, 698, 728
Bsc4I	CCNNNNNNNGG	3 cut(s)	413, 1481, 1675
Bse118I	RCCGGY	2 cut(s)	1001, 1016
Bse1I	ACTGG	3 cut(s)	135, 518, 1450
Bse21I	CCTNAGG	1 cut(s)	456
BseBI	CCWGG	5 cut(s)	118, 257, 332, 1446, 1476
BseDI	CCNNGG	6 cut(s)	116, 580, 994, 1160, 1542, 1669
BseGI	GGATG	4 cut(s)	76, 600, 715, 1204
BseLI	CCNNNNNNNGG	3 cut(s)	413, 1481, 1675
BseMII	CTCAG	7 cut(s)	143, 470, 666, 742, 966, 1560, 1689
BseNI	ACTGG	3 cut(s)	135, 518, 1450
BseRI	GAGGAG	2 cut(s)	654, 1586
BseXI	GCAGC	7 cut(s)	288, 846, 956, 1168, 1550, 1627, 1639
Bsh1236I	CGCG	2 cut(s)	1383, 1684
BshFI	GGCC	3 cut(s)	222, 517, 1449
BshNI	GGYRCC	3 cut(s)	450, 998, 1013
BshTI	ACCGGT	1 cut(s)	1016
BsiHKAI	GWGCWC	2 cut(s)	330, 981
BsiSI	CCGG	2 cut(s)	1002, 1017
BslFI	GGGAC	6 cut(s)	215, 281, 582, 697, 925, 1722
BslI	CCNNNNNNNGG	3 cut(s)	413, 1481, 1675
BsmAI	GTCTC	2 cut(s)	1070, 1691
BsmBI	CGTCTC	1 cut(s)	1691
BsmFI	GGGAC	6 cut(s)	215, 281, 582, 697, 925, 1722
BsnI	GGCC	3 cut(s)	222, 517, 1449
Bso31I	GGTCTC	1 cut(s)	1070
Bsp1286I	GDGCHC	3 cut(s)	330, 981, 1168
Bsp1407I	TGTACA	3 cut(s)	624, 820, 1731
Bsp143I	GATC	9 cut(s)	33, 157, 763, 1044, 1089, 1189, 1235, 1272, 1411
BspACI	CCGC	7 cut(s)	57, 60, 299, 1350, 1398, 1556, 1756
BspANI	GGCC	3 cut(s)	222, 517, 1449
BspCNI	CTCAG	7 cut(s)	144, 469, 665, 741, 967, 1561, 1690
BspFNI	CGCG	2 cut(s)	1383, 1684
BspHI	TCATGA	2 cut(s)	780, 1168
BspLI	GGNNCC	4 cut(s)	452, 967, 1000, 1015
BspPI	GGATC	5 cut(s)	152, 771, 1197, 1243, 1406
BspT107I	GGYRCC	3 cut(s)	450, 998, 1013
BspTNI	GGTCTC	1 cut(s)	1070
BsrFI	RCCGGY	2 cut(s)	1001, 1016
BsrGI	TGTACA	3 cut(s)	624, 820, 1731
BsrI	ACTGG	3 cut(s)	135, 518, 1450
BssAI	RCCGGY	2 cut(s)	1001, 1016
BssECI	CCNNGG	6 cut(s)	116, 580, 994, 1160, 1542, 1669
BssMI	GATC	9 cut(s)	33, 157, 763, 1044, 1089, 1189, 1235, 1272, 1411
BssNI	GRCGYC	1 cut(s)	451
BssT1I	CCWWGG	3 cut(s)	994, 1160, 1669
Bst2UI	CCWGG	5 cut(s)	118, 257, 332, 1446, 1476
Bst4CI	ACNGT	8 cut(s)	28, 52, 412, 529, 825, 1225, 1373, 1736
Bst6I	CTCTTC	2 cut(s)	1068, 1146
BstACI	GRCGYC	1 cut(s)	451
BstAPI	GCANNNNNTGC	1 cut(s)	1391
BstAUI	TGTACA	3 cut(s)	624, 820, 1731
BstC8I	GCNNGC	5 cut(s)	7, 180, 424, 1003, 1396
BstDEI	CTNAG	7 cut(s)	152, 456, 652, 728, 975, 1569, 1698
BstEII	GGTNACC	1 cut(s)	575
BstF5I	GGATG	4 cut(s)	76, 600, 715, 1204
BstFNI	CGCG	2 cut(s)	1383, 1684
BstH2I	RGCGCY	1 cut(s)	454
BstHHI	GCGC	2 cut(s)	453, 1385
BstKTI	GATC	9 cut(s)	36, 160, 766, 1047, 1092, 1192, 1238, 1275, 1414
BstMAI	GTCTC	2 cut(s)	1070, 1691
BstMBI	GATC	9 cut(s)	33, 157, 763, 1044, 1089, 1189, 1235, 1272, 1411
BstMWI	GCNNNNNNNGC	8 cut(s)	1007, 1208, 1358, 1391, 1547, 1637, 1649, 1658
BstNI	CCWGG	5 cut(s)	118, 257, 332, 1446, 1476
BstNSI	RCATGY	3 cut(s)	660, 1531, 1732
BstPI	GGTNACC	1 cut(s)	575
BstSCI	CCNGG	5 cut(s)	116, 255, 330, 1444, 1474
BstSFI	CTRYAG	1 cut(s)	921
BstUI	CGCG	2 cut(s)	1383, 1684
BstV1I	GCAGC	7 cut(s)	288, 846, 956, 1168, 1550, 1627, 1639
BstV2I	GAAGAC	2 cut(s)	105, 883
BstX2I	RGATCY	5 cut(s)	157, 763, 1044, 1089, 1189
BstYI	RGATCY	5 cut(s)	157, 763, 1044, 1089, 1189
Bsu36I	CCTNAGG	1 cut(s)	456
BsuRI	GGCC	3 cut(s)	222, 517, 1449
BtsCI	GGATG	4 cut(s)	76, 600, 715, 1204
BtsI	GCAGTG	1 cut(s)	87
BtsIMutI	CAGTG	3 cut(s)	87, 142, 1378
Cac8I	GCNNGC	5 cut(s)	7, 180, 424, 1003, 1396
CaiI	CAGNNNCTG	3 cut(s)	239, 703, 1591
CciI	TCATGA	2 cut(s)	780, 1168
CfoI	GCGC	2 cut(s)	453, 1385
Cfr10I	RCCGGY	2 cut(s)	1001, 1016
CseI	GACGC	2 cut(s)	212, 1673
Csp6I	GTAC	6 cut(s)	625, 821, 1014, 1347, 1423, 1732
CspAI	ACCGGT	1 cut(s)	1016
CviAII	CATG	8 cut(s)	313, 657, 781, 970, 1169, 1175, 1528, 1729
CviQI	GTAC	6 cut(s)	625, 821, 1014, 1347, 1423, 1732
DdeI	CTNAG	7 cut(s)	152, 456, 652, 728, 975, 1569, 1698
DinI	GGCGCC	1 cut(s)	452
DpnI	GATC	9 cut(s)	35, 159, 765, 1046, 1091, 1191, 1237, 1274, 1413
DpnII	GATC	9 cut(s)	33, 157, 763, 1044, 1089, 1189, 1235, 1272, 1411
DrdI	GACNNNNNNGTC	1 cut(s)	107
DriI	GACNNNNNGTC	1 cut(s)	43
DseDI	GACNNNNNNGTC	1 cut(s)	107
EaeI	YGGCCR	1 cut(s)	1447
Eam1104I	CTCTTC	2 cut(s)	1068, 1146
Eam1105I	GACNNNNNGTC	1 cut(s)	43
EarI	CTCTTC	2 cut(s)	1068, 1146
Ecl136II	GAGCTC	1 cut(s)	328
Eco130I	CCWWGG	3 cut(s)	994, 1160, 1669
Eco24I	GRGCYC	2 cut(s)	330, 1168
Eco31I	GGTCTC	1 cut(s)	1070
Eco53kI	GAGCTC	1 cut(s)	328
Eco57I	CTGAAG	3 cut(s)	430, 1374, 1730
Eco81I	CCTNAGG	1 cut(s)	456
Eco91I	GGTNACC	1 cut(s)	575
EcoICRI	GAGCTC	1 cut(s)	328
EcoO65I	GGTNACC	1 cut(s)	575
EcoRI	GAATTC	1 cut(s)	600
EcoRII	CCWGG	5 cut(s)	116, 255, 330, 1444, 1474
EcoT14I	CCWWGG	3 cut(s)	994, 1160, 1669
EcoT22I	ATGCAT	1 cut(s)	1465
EcoT38I	GRGCYC	2 cut(s)	330, 1168
EgeI	GGCGCC	1 cut(s)	452
EheI	GGCGCC	1 cut(s)	452
ErhI	CCWWGG	3 cut(s)	994, 1160, 1669
Esp3I	CGTCTC	1 cut(s)	1691
FaeI	CATG	8 cut(s)	316, 660, 784, 973, 1172, 1178, 1531, 1732
FalI	AAGNNNNNCTT	4 cut(s)	90, 122, 1125, 1157
FaqI	GGGAC	6 cut(s)	215, 281, 582, 697, 925, 1722
FatI	CATG	8 cut(s)	312, 656, 780, 969, 1168, 1174, 1527, 1728
FauI	CCCGC	2 cut(s)	306, 1549
FauNDI	CATATG	1 cut(s)	1663
FblI	GTMKAC	1 cut(s)	399
Fnu4HI	GCNGC	9 cut(s)	58, 61, 302, 860, 945, 1157, 1539, 1641, 1653
FokI	GGATG	4 cut(s)	83, 607, 722, 1211
FriOI	GRGCYC	2 cut(s)	330, 1168
Fsp4HI	GCNGC	9 cut(s)	58, 61, 302, 860, 945, 1157, 1539, 1641, 1653
FspBI	CTAG	2 cut(s)	497, 1562
GlaI	GCGC	2 cut(s)	452, 1384
GluI	GCNGC	9 cut(s)	58, 61, 302, 860, 945, 1157, 1539, 1641, 1653
GsuI	CTGGAG	5 cut(s)	216, 239, 314, 725, 1524
HaeII	RGCGCY	1 cut(s)	454
HaeIII	GGCC	3 cut(s)	222, 517, 1449
HapII	CCGG	2 cut(s)	1002, 1017
HgaI	GACGC	2 cut(s)	212, 1673
HhaI	GCGC	2 cut(s)	453, 1385
Hin1I	GRCGYC	1 cut(s)	451
Hin1II	CATG	8 cut(s)	316, 660, 784, 973, 1172, 1178, 1531, 1732
Hin6I	GCGC	2 cut(s)	451, 1383
HinP1I	GCGC	2 cut(s)	451, 1383
HincII	GTYRAC	1 cut(s)	321
HindII	GTYRAC	1 cut(s)	321
HindIII	AAGCTT	2 cut(s)	1359, 1548
HinfI	GANTC	6 cut(s)	20, 726, 1103, 1136, 1479, 1702
HpaI	GTTAAC	1 cut(s)	321
HpaII	CCGG	2 cut(s)	1002, 1017
HphI	GGTGA	5 cut(s)	92, 103, 428, 566, 587
Hpy166II	GTNNAC	6 cut(s)	48, 321, 400, 557, 627, 1519
Hpy8I	GTNNAC	6 cut(s)	48, 321, 400, 557, 627, 1519
Hpy99I	CGWCG	1 cut(s)	56
HpyAV	CCTTC	3 cut(s)	233, 478, 1300
HpyCH4III	ACNGT	8 cut(s)	28, 52, 412, 529, 825, 1225, 1373, 1736
HpyCH4IV	ACGT	1 cut(s)	559
HpyCH4V	TGCA	8 cut(s)	182, 304, 839, 932, 1463, 1583, 1655, 1728
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1007, 1208, 1358, 1391, 1547, 1637, 1649, 1658
HpyF3I	CTNAG	7 cut(s)	152, 456, 652, 728, 975, 1569, 1698
HpySE526I	ACGT	1 cut(s)	559
Hsp92I	GRCGYC	1 cut(s)	451
Hsp92II	CATG	8 cut(s)	316, 660, 784, 973, 1172, 1178, 1531, 1732
HspAI	GCGC	2 cut(s)	451, 1383
KasI	GGCGCC	1 cut(s)	450
KpnI	GGTACC	1 cut(s)	1017
KroI	GCCGGC	1 cut(s)	1001
KroNI	GCCGGC	1 cut(s)	1003
KspAI	GTTAAC	1 cut(s)	321
Kzo9I	GATC	9 cut(s)	33, 157, 763, 1044, 1089, 1189, 1235, 1272, 1411
LmnI	GCTCC	3 cut(s)	325, 333, 1505
Lsp1109I	GCAGC	7 cut(s)	288, 846, 956, 1168, 1550, 1627, 1639
LweI	GCATC	3 cut(s)	450, 926, 1189
MaeI	CTAG	2 cut(s)	497, 1562
MaeII	ACGT	1 cut(s)	559
MaeIII	GTNAC	5 cut(s)	13, 109, 544, 575, 667
MalI	GATC	9 cut(s)	35, 159, 765, 1046, 1091, 1191, 1237, 1274, 1413
MboI	GATC	9 cut(s)	33, 157, 763, 1044, 1089, 1189, 1235, 1272, 1411
MflI	RGATCY	5 cut(s)	157, 763, 1044, 1089, 1189
MhlI	GDGCHC	3 cut(s)	330, 981, 1168
MlsI	TGGCCA	1 cut(s)	1449
MluCI	AATT	7 cut(s)	383, 431, 600, 953, 1212, 1386, 1576
MluI	ACGCGT	1 cut(s)	1682
MluNI	TGGCCA	1 cut(s)	1449
Mly113I	GGCGCC	1 cut(s)	451
MlyI	GAGTC	1 cut(s)	720
MmeI	TCCRAC	5 cut(s)	307, 431, 1228, 1347, 1479
Mox20I	TGGCCA	1 cut(s)	1449
Mph1103I	ATGCAT	1 cut(s)	1465
MroNI	GCCGGC	1 cut(s)	1001
MroXI	GAANNNNTTC	2 cut(s)	387, 1624
MscI	TGGCCA	1 cut(s)	1449
MseI	TTAA	5 cut(s)	320, 951, 1152, 1185, 1215
MslI	CAYNNNNRTG	1 cut(s)	1173
Msp20I	TGGCCA	1 cut(s)	1449
MspA1I	CMGCKG	4 cut(s)	301, 1352, 1398, 1758
MspI	CCGG	2 cut(s)	1002, 1017
MspR9I	CCNGG	5 cut(s)	118, 257, 332, 1446, 1476
MvaI	CCWGG	5 cut(s)	118, 257, 332, 1446, 1476
MvnI	CGCG	2 cut(s)	1383, 1684
MwoI	GCNNNNNNNGC	8 cut(s)	1007, 1208, 1358, 1391, 1547, 1637, 1649, 1658
NaeI	GCCGGC	1 cut(s)	1003
NarI	GGCGCC	1 cut(s)	451
NdeI	CATATG	1 cut(s)	1663
NdeII	GATC	9 cut(s)	33, 157, 763, 1044, 1089, 1189, 1235, 1272, 1411
NgoMIV	GCCGGC	1 cut(s)	1001
NlaIII	CATG	8 cut(s)	316, 660, 784, 973, 1172, 1178, 1531, 1732
NlaIV	GGNNCC	4 cut(s)	452, 967, 1000, 1015
NmeAIII	GCCGAG	1 cut(s)	1744
NmuCI	GTSAC	5 cut(s)	13, 109, 544, 575, 667
NsiI	ATGCAT	1 cut(s)	1465
NspI	RCATGY	3 cut(s)	660, 1531, 1732
PagI	TCATGA	2 cut(s)	780, 1168
PcsI	WCGNNNNNNNCGW	1 cut(s)	60
PdiI	GCCGGC	1 cut(s)	1003
PdmI	GAANNNNTTC	2 cut(s)	387, 1624
PfeI	GAWTC	5 cut(s)	20, 1103, 1136, 1479, 1702
PfoI	TCCNGGA	3 cut(s)	255, 330, 1474
PinAI	ACCGGT	1 cut(s)	1016
PkrI	GCNGC	9 cut(s)	59, 62, 303, 861, 946, 1158, 1540, 1642, 1654
PleI	GAGTC	1 cut(s)	720
PluTI	GGCGCC	1 cut(s)	454
PpsI	GAGTC	1 cut(s)	720
Psp124BI	GAGCTC	1 cut(s)	330
Psp6I	CCWGG	5 cut(s)	116, 255, 330, 1444, 1474
PspEI	GGTNACC	1 cut(s)	575
PspGI	CCWGG	5 cut(s)	116, 255, 330, 1444, 1474
PspN4I	GGNNCC	4 cut(s)	452, 967, 1000, 1015
PstNI	CAGNNNCTG	3 cut(s)	239, 703, 1591
PsuI	RGATCY	5 cut(s)	157, 763, 1044, 1089, 1189
RsaI	GTAC	6 cut(s)	626, 822, 1015, 1348, 1424, 1733
RsaNI	GTAC	6 cut(s)	625, 821, 1014, 1347, 1423, 1732
RseI	CAYNNNNRTG	1 cut(s)	1173
SacI	GAGCTC	1 cut(s)	330
SaqAI	TTAA	5 cut(s)	320, 951, 1152, 1185, 1215
SatI	GCNGC	9 cut(s)	58, 61, 302, 860, 945, 1157, 1539, 1641, 1653
Sau3AI	GATC	9 cut(s)	33, 157, 763, 1044, 1089, 1189, 1235, 1272, 1411
SchI	GAGTC	1 cut(s)	720
ScrFI	CCNGG	5 cut(s)	118, 257, 332, 1446, 1476
SduI	GDGCHC	3 cut(s)	330, 981, 1168
SfaNI	GCATC	3 cut(s)	450, 926, 1189
SfcI	CTRYAG	1 cut(s)	921
SfoI	GGCGCC	1 cut(s)	452
SmiMI	CAYNNNNRTG	1 cut(s)	1173
SmlI	CTYRAG	1 cut(s)	1040
SmoI	CTYRAG	1 cut(s)	1040
Sse9I	AATT	7 cut(s)	383, 431, 600, 953, 1212, 1386, 1576
SsiI	CCGC	7 cut(s)	57, 60, 299, 1350, 1398, 1556, 1756
SspDI	GGCGCC	1 cut(s)	450
SspMI	CTAG	2 cut(s)	497, 1562
SstI	GAGCTC	1 cut(s)	330
StyD4I	CCNGG	5 cut(s)	116, 255, 330, 1444, 1474
StyI	CCWWGG	3 cut(s)	994, 1160, 1669
TaaI	ACNGT	8 cut(s)	28, 52, 412, 529, 825, 1225, 1373, 1736
TaiI	ACGT	1 cut(s)	562
TaqI	TCGA	4 cut(s)	36, 378, 855, 1275
TaqII	GACCGA	1 cut(s)	1472
TasI	AATT	7 cut(s)	383, 431, 600, 953, 1212, 1386, 1576
TatI	WGTACW	4 cut(s)	624, 820, 1422, 1731
TauI	GCSGC	2 cut(s)	60, 63
TfiI	GAWTC	5 cut(s)	20, 1103, 1136, 1479, 1702
Tru1I	TTAA	5 cut(s)	320, 951, 1152, 1185, 1215
Tru9I	TTAA	5 cut(s)	320, 951, 1152, 1185, 1215
TscAI	CASTG	3 cut(s)	94, 142, 1378
TseFI	GTSAC	5 cut(s)	13, 109, 544, 575, 667
TseI	GCWGC	7 cut(s)	301, 859, 944, 1156, 1538, 1640, 1652
Tsp45I	GTSAC	5 cut(s)	13, 109, 544, 575, 667
TspDTI	ATGAA	4 cut(s)	287, 769, 1185, 1429
TspGWI	ACGGA	4 cut(s)	32, 118, 338, 1502
TspRI	CASTG	3 cut(s)	94, 142, 1378
XapI	RAATTY	3 cut(s)	431, 600, 1576
XbaI	TCTAGA	1 cut(s)	496
XceI	RCATGY	3 cut(s)	660, 1531, 1732
XmiI	GTMKAC	1 cut(s)	399
XmnI	GAANNNNTTC	2 cut(s)	387, 1624
XspI	CTAG	2 cut(s)	497, 1562
Zsp2I	ATGCAT	1 cut(s)	1465

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.