Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0005972

Physical Location & Seq

Genomic Coordinates Reverse (-)

5220946 .. 5227272

Gene Structure 6327 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G005550.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1929 bp

ATGCTTATATGCTTTGGGACCATACTCAAACCAAGACACCCAAACAAAAGCTTGACTTTCTTGATTTCTGGTCACTTGGACAAAAGCTCAGAATACTGTGCTCTCAGCCAAAAAAAGTGGAGAAGTTCAAAAGAGCGAGTTGAGTCGGTGAGAATGGAGAGCGCACTCAAGGAGGTGAGGGATGGTGTTTCTGTGCTTGACCTGGACCCCACAGCCGCCGTCGGTGGCGGCGTTGAGGACGTCTACGGTGAGGATGCTGCCACCGAGGACCAACTCGTCACTCCCTGGACTTTCTCTGTAGCCAGTGGGTACTCTCTGCTGAGAGATCCTCAATACAACAAAGGGCTAGCATTTACCGAGAAAGAGAGGGATGCTCATTACTTGCGCGGTCTTCTGCCTCCGGCTACTTCCACTCAACAACTTCAGGAGAAGAAGCTGATGCACAATATCAGACAATACCAAGTGCCACTGCAAAAATATATGGCACTGACCGAACTCCAGGAGAGGAATGAGAGGCTGTTCTACAAACTTCTCATTGATAATGTCGAGGAATTGCTCCCGATTGTATATACTCCAACTGTTGGTGAGGCTTGCCAGAAATATGGAAGCATTTTCAGACGCCCTCAGGGTCTTTACATAAGTCTGAAAGAGAAAGGTAGAATTCTCGAGGTATTGAAGAACTGGCCTGAAAGGACTATTCAAGTTATTGTTGTCACTGATGGTGAGCGTATATTGGGACTTGGTGACCTTGGTTGTCAGGGCATGGGAATTCCTGTGGGGAAGTTGGCTTTGTACACGGCTCTTGGAGGAGTTCGTCCCTCAGCATGTTTGCCTATAACCATTGATGTTGGGACAAATAATGAGCAGTTGCTGAAGGATGAGTTCTACATTGGGCTCAGACAAAAGAGAGCAACTGGAAAGGAATATGCTGAGCTTTTACATGAGTTCATGGGTGCTGTGAAGCAGAACTATGGCGAAAAAGTAGTTGTACAGTTTGAAGATTTCGCTAACCACAATGCCTTTGAGCTGCTGGCAAAATATCGAAATACTCATCTCGTCTTCAATGATGACATACAGGGGACTGCTGCTGTAGTTCTTGCTGGAGTTGTGGCGGCACTAAAGTTGATAGGTGGTACCCTGTCTGAGCACAAGTTTTTGTTCCTCGGTGCTGGGGAAGCTGGGACAGGTATAGCAGAGCTAATAGCTCTTGAGATCTCAAAAAAGACAAATGTTCCTGTGGAAGAGACGCGCAAAAAGATCTGGCTTGTTGATTCAAAGGGCCTAATTGTTAGCTCTCGTAAAGAATCTCTTCAAAATTTTAAGAAGCCTTGGGCTCATGAGCATGAACCCGTTAAGGATCTCATTGATGCTGTCAAGGCAATTAAACCAACAGTTCTTATTGGAACATCTGGTATTGGAAAAACATTCACAAAAGAAGTGATTGAGGCACTAGCTTCCTTCAATGAGAAACCTCTCATTTTGGCTCTTTCCAACCCAACATCACAGTCAGAATGTACAGCTGAAGAAGCTTATACTTGGACTAAGGGTCGTGCAATTTTTGCTAGTGGAAGTCCTTTCGATCCTTTTGAATATGACGGCAAAGTTTATATTCCTGGCCAGTCCAACAATGCTTACATTTTCCCCGGATTTGGTCTTGGTTTGGTCATCTCTGGAGCAATTCGGGTGCATGATGATATGCTTCTAGCAGCTTCTGAGGCCCTGGCGGGACAAGTTTCAAAGGAGAATTTCGATAAAGGACTTATTTATCCACCATTCTCTAAAATCAGAAAGATTTCAGCTGAAATAGCTGCTAATGTAGCTGCCAAGGCATATGAACTTGGTATTGCTACACGACTCCCTCGTCCAGAAAATCTTGTGAAGTATGCTGAGAGTTGCATGTATAGTCCTCTCTACAGAAGCTACCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006090 GO:0006108 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0051259 GO:0051260 GO:0055114 GO:0065003 GO:0071704 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

642

Amino Acids

71.11

Weight (kDa)

7.57

Isoelectric Point (pI)

37.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	167 - 348	1e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	358 - 611	2.6e-96	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19900 AT2G19900
fragaria_vesca	FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000 FvH4_1g06000
malus_domestica	MD02G1064800.v1.1 MD15G1195500.v1.1
prunus_persica	Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1 Prupe.7G221100_v2.0.a1
pyrus_communis	pycom02g05090 pycom15g17470
rosa_chinensis	RchiOBHm_Chr2g0091741
rosa_laevigata	RLG00000016248
rosa_roxburghii	Rroxscaffold_2G00149780
rosa_rugosa	Rorug02G0020300
rosa_samantha	Rh2BG064200 Rh2CG065800 Rh2DG064300
rosa_wichuraiana	Rw2G005550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	275, 1860
AatII	GACGTC	1 cut(s)	243
Acc65I	GGTACC	1 cut(s)	1133
AccB1I	GGYRCC	1 cut(s)	1133
AccB7I	CCANNNNNTGG	1 cut(s)	581
AccI	GTMKAC	1 cut(s)	243
AccII	CGCG	2 cut(s)	387, 1249
AciI	CCGC	5 cut(s)	216, 228, 387, 1112, 1724
AclWI	GGATC	3 cut(s)	320, 1365, 1574
AcoI	YGGCCR	1 cut(s)	1615
AcsI	RAATTY	4 cut(s)	660, 768, 1315, 1744
AcuI	CTGAAG	3 cut(s)	407, 893, 1542
AcyI	GRCGYC	2 cut(s)	240, 619
AfaI	GTAC	5 cut(s)	311, 794, 990, 1135, 1516
AfiI	CCNNNNNNNGG	2 cut(s)	581, 1649
AgeI	ACCGGT	1 cut(s)	1923
AhdI	GACNNNNNGTC	1 cut(s)	1545
AjnI	CCWGG	5 cut(s)	201, 284, 498, 1612, 1719
Alw21I	GWGCWC	2 cut(s)	103, 1149
Alw26I	GTCTC	1 cut(s)	1238
AlwI	GGATC	3 cut(s)	320, 1365, 1574
AlwNI	CAGNNNCTG	2 cut(s)	871, 1712
Ama87I	CYCGRG	1 cut(s)	665
AoxI	GGCC	4 cut(s)	683, 1279, 1615, 1716
ApeKI	GCWGC	6 cut(s)	257, 1027, 1085, 1706, 1808, 1820
ApoI	RAATTY	4 cut(s)	660, 768, 1315, 1744
AsiGI	ACCGGT	1 cut(s)	1923
Asp700I	GAANNNNTTC	2 cut(s)	1308, 1792
Asp718I	GGTACC	1 cut(s)	1133
AspLEI	GCGC	3 cut(s)	164, 387, 1251
AspS9I	GGNCC	5 cut(s)	18, 205, 268, 1279, 1717
AsuC2I	CCSGG	1 cut(s)	1644
AsuHPI	GGTGA	6 cut(s)	160, 187, 260, 596, 734, 755
AsuNHI	GCTAGC	1 cut(s)	346
AvaI	CYCGRG	1 cut(s)	665
AvaII	GGWCC	3 cut(s)	18, 205, 268
AxyI	CCTNAGG	1 cut(s)	624
BalI	TGGCCA	1 cut(s)	1617
BanI	GGYRCC	1 cut(s)	1133
BanII	GRGCYC	2 cut(s)	897, 1336
BbsI	GAAGAC	2 cut(s)	383, 1051
Bbv12I	GWGCWC	2 cut(s)	103, 1149
BbvCI	CCTCAGC	1 cut(s)	820
BbvI	GCAGC	6 cut(s)	244, 1014, 1072, 1718, 1795, 1807
BccI	CCATC	2 cut(s)	176, 713
BceAI	ACGGC	3 cut(s)	203, 813, 1612
BciT130I	CCWGG	5 cut(s)	203, 286, 500, 1614, 1721
BcnI	CCSGG	1 cut(s)	1644
BcoDI	GTCTC	1 cut(s)	1238
BfaI	CTAG	4 cut(s)	347, 1451, 1563, 1703
BfmI	CTRYAG	3 cut(s)	297, 1089, 1912
BglII	AGATCT	2 cut(s)	1212, 1257
BisI	GCNGC	9 cut(s)	216, 229, 258, 1028, 1086, 1113, 1707, 1809, 1821
BlpI	GCTNAGC	1 cut(s)	930
BlsI	GCNGC	9 cut(s)	217, 230, 259, 1029, 1087, 1114, 1708, 1810, 1822
Bme1390I	CCNGG	6 cut(s)	203, 286, 500, 1614, 1644, 1721
Bme18I	GGWCC	3 cut(s)	18, 205, 268
BmeRI	GACNNNNNGTC	1 cut(s)	1545
BmeT110I	CYCGRG	1 cut(s)	665
BmgT120I	GGNCC	5 cut(s)	18, 205, 268, 1279, 1717
BmiI	GGNNCC	3 cut(s)	19, 207, 1135
BmrFI	CCNGG	6 cut(s)	203, 286, 500, 1614, 1644, 1721
BmsI	GCATC	4 cut(s)	244, 361, 429, 1357
BmtI	GCTAGC	1 cut(s)	350
BpiI	GAAGAC	2 cut(s)	383, 1051
BplI	GAGNNNNNCTC	4 cut(s)	313, 345, 358, 390
BpmI	CTGGAG	3 cut(s)	482, 1122, 1692
Bpu10I	CCTNAGC	1 cut(s)	820
Bpu1102I	GCTNAGC	1 cut(s)	930
BpuEI	CTTGAG	2 cut(s)	152, 1229
BpuMI	CCSGG	1 cut(s)	1644
BsaHI	GRCGYC	2 cut(s)	240, 619
BsaJI	CCNNGG	8 cut(s)	264, 284, 748, 1162, 1328, 1642, 1719, 1824
BsaWI	WCCGGW	1 cut(s)	1923
Bsc4I	CCNNNNNNNGG	2 cut(s)	581, 1649
Bse118I	RCCGGY	1 cut(s)	1923
Bse1I	ACTGG	4 cut(s)	303, 686, 919, 1618
Bse21I	CCTNAGG	1 cut(s)	624
BseBI	CCWGG	5 cut(s)	203, 286, 500, 1614, 1721
BseDI	CCNNGG	8 cut(s)	264, 284, 748, 1162, 1328, 1642, 1719, 1824
BseGI	GGATG	4 cut(s)	187, 259, 376, 883
BseLI	CCNNNNNNNGG	2 cut(s)	581, 1649
BseNI	ACTGG	4 cut(s)	303, 686, 919, 1618
BseRI	GAGGAG	1 cut(s)	822
BseXI	GCAGC	6 cut(s)	244, 1014, 1072, 1718, 1795, 1807
BseYI	CCCAGC	2 cut(s)	1169, 1178
Bsh1236I	CGCG	2 cut(s)	387, 1249
BshFI	GGCC	4 cut(s)	685, 1281, 1617, 1718
BshNI	GGYRCC	1 cut(s)	1133
BshTI	ACCGGT	1 cut(s)	1923
BsiHKAI	GWGCWC	2 cut(s)	103, 1149
BsiHKCI	CYCGRG	1 cut(s)	665
BsiSI	CCGG	3 cut(s)	401, 1644, 1924
BslFI	GGGAC	7 cut(s)	31, 750, 801, 865, 1093, 1195, 1740
BslI	CCNNNNNNNGG	2 cut(s)	581, 1649
BsmAI	GTCTC	1 cut(s)	1238
BsmBI	CGTCTC	1 cut(s)	1238
BsmFI	GGGAC	7 cut(s)	31, 750, 801, 865, 1093, 1195, 1740
BsnI	GGCC	4 cut(s)	685, 1281, 1617, 1718
BsoBI	CYCGRG	1 cut(s)	665
Bsp1286I	GDGCHC	4 cut(s)	103, 897, 1149, 1336
Bsp1407I	TGTACA	3 cut(s)	792, 988, 1514
Bsp143I	GATC	5 cut(s)	325, 1212, 1257, 1357, 1579
Bsp1720I	GCTNAGC	1 cut(s)	930
BspACI	CCGC	5 cut(s)	216, 228, 387, 1112, 1724
BspANI	GGCC	4 cut(s)	685, 1281, 1617, 1718
BspFNI	CGCG	2 cut(s)	387, 1249
BspHI	TCATGA	1 cut(s)	1336
BspLI	GGNNCC	3 cut(s)	19, 207, 1135
BspOI	GCTAGC	1 cut(s)	350
BspPI	GGATC	3 cut(s)	320, 1365, 1574
BspT107I	GGYRCC	1 cut(s)	1133
BsrFI	RCCGGY	1 cut(s)	1923
BsrGI	TGTACA	3 cut(s)	792, 988, 1514
BsrI	ACTGG	4 cut(s)	303, 686, 919, 1618
BssAI	RCCGGY	1 cut(s)	1923
BssECI	CCNNGG	8 cut(s)	264, 284, 748, 1162, 1328, 1642, 1719, 1824
BssMI	GATC	5 cut(s)	325, 1212, 1257, 1357, 1579
BssNI	GRCGYC	2 cut(s)	240, 619
BssT1I	CCWWGG	3 cut(s)	748, 1328, 1824
Bst2UI	CCWGG	5 cut(s)	203, 286, 500, 1614, 1721
Bst4CI	ACNGT	6 cut(s)	98, 248, 580, 993, 1393, 1506
Bst6I	CTCTTC	2 cut(s)	1236, 1314
BstACI	GRCGYC	2 cut(s)	240, 619
BstAPI	GCANNNNNTGC	1 cut(s)	1559
BstAUI	TGTACA	3 cut(s)	792, 988, 1514
BstC8I	GCNNGC	3 cut(s)	348, 592, 1032
BstEII	GGTNACC	1 cut(s)	743
BstF5I	GGATG	4 cut(s)	187, 259, 376, 883
BstFNI	CGCG	2 cut(s)	387, 1249
BstHHI	GCGC	3 cut(s)	164, 387, 1251
BstKTI	GATC	5 cut(s)	328, 1215, 1260, 1360, 1582
BstMAI	GTCTC	1 cut(s)	1238
BstMBI	GATC	5 cut(s)	325, 1212, 1257, 1357, 1579
BstMWI	GCNNNNNNNGC	8 cut(s)	1175, 1376, 1526, 1559, 1715, 1805, 1817, 1826
BstNI	CCWGG	5 cut(s)	203, 286, 500, 1614, 1721
BstNSI	RCATGY	2 cut(s)	828, 1900
BstPI	GGTNACC	1 cut(s)	743
BstSCI	CCNGG	6 cut(s)	201, 284, 498, 1612, 1642, 1719
BstSFI	CTRYAG	3 cut(s)	297, 1089, 1912
BstUI	CGCG	2 cut(s)	387, 1249
BstV1I	GCAGC	6 cut(s)	244, 1014, 1072, 1718, 1795, 1807
BstV2I	GAAGAC	2 cut(s)	383, 1051
BstX2I	RGATCY	4 cut(s)	325, 1212, 1257, 1357
BstXI	CCANNNNNNTGG	1 cut(s)	602
BstYI	RGATCY	4 cut(s)	325, 1212, 1257, 1357
Bsu36I	CCTNAGG	1 cut(s)	624
BsuRI	GGCC	4 cut(s)	685, 1281, 1617, 1718
BtsCI	GGATG	4 cut(s)	187, 259, 376, 883
BtsI	GCAGTG	1 cut(s)	467
BtsIMutI	CAGTG	4 cut(s)	310, 467, 485, 714
Cac8I	GCNNGC	3 cut(s)	348, 592, 1032
CaiI	CAGNNNCTG	2 cut(s)	871, 1712
CciI	TCATGA	1 cut(s)	1336
CfoI	GCGC	3 cut(s)	164, 387, 1251
Cfr10I	RCCGGY	1 cut(s)	1923
Cfr13I	GGNCC	5 cut(s)	18, 205, 268, 1279, 1717
CseI	GACGC	2 cut(s)	627, 1255
Csp6I	GTAC	5 cut(s)	310, 793, 989, 1134, 1515
CspAI	ACCGGT	1 cut(s)	1923
CspCI	CAANNNNNGTGG	2 cut(s)	98, 133
CviAII	CATG	8 cut(s)	763, 825, 941, 949, 1337, 1343, 1688, 1897
CviQI	GTAC	5 cut(s)	310, 793, 989, 1134, 1515
DpnI	GATC	5 cut(s)	327, 1214, 1259, 1359, 1581
DpnII	GATC	5 cut(s)	325, 1212, 1257, 1357, 1579
DrdI	GACNNNNNNGTC	2 cut(s)	275, 1860
DriI	GACNNNNNGTC	1 cut(s)	1545
DseDI	GACNNNNNNGTC	2 cut(s)	275, 1860
EaeI	YGGCCR	1 cut(s)	1615
Eam1104I	CTCTTC	2 cut(s)	1236, 1314
Eam1105I	GACNNNNNGTC	1 cut(s)	1545
EarI	CTCTTC	2 cut(s)	1236, 1314
Eco130I	CCWWGG	3 cut(s)	748, 1328, 1824
Eco24I	GRGCYC	2 cut(s)	897, 1336
Eco47I	GGWCC	3 cut(s)	18, 205, 268
Eco57I	CTGAAG	3 cut(s)	407, 893, 1542
Eco81I	CCTNAGG	1 cut(s)	624
Eco88I	CYCGRG	1 cut(s)	665
Eco91I	GGTNACC	1 cut(s)	743
EcoO109I	RGGNCCY	2 cut(s)	1279, 1717
EcoO65I	GGTNACC	1 cut(s)	743
EcoRI	GAATTC	2 cut(s)	660, 768
EcoRII	CCWGG	5 cut(s)	201, 284, 498, 1612, 1719
EcoT14I	CCWWGG	3 cut(s)	748, 1328, 1824
EcoT38I	GRGCYC	2 cut(s)	897, 1336
ErhI	CCWWGG	3 cut(s)	748, 1328, 1824
Esp3I	CGTCTC	1 cut(s)	1238
FaeI	CATG	8 cut(s)	766, 828, 944, 952, 1340, 1346, 1691, 1900
FalI	AAGNNNNNCTT	4 cut(s)	40, 72, 1293, 1325
FaqI	GGGAC	7 cut(s)	31, 750, 801, 865, 1093, 1195, 1740
FatI	CATG	8 cut(s)	762, 824, 940, 948, 1336, 1342, 1687, 1896
FauI	CCCGC	1 cut(s)	1717
FauNDI	CATATG	1 cut(s)	1831
FblI	GTMKAC	1 cut(s)	243
Fnu4HI	GCNGC	9 cut(s)	216, 229, 258, 1028, 1086, 1113, 1707, 1809, 1821
FokI	GGATG	4 cut(s)	194, 266, 383, 890
FriOI	GRGCYC	2 cut(s)	897, 1336
Fsp4HI	GCNGC	9 cut(s)	216, 229, 258, 1028, 1086, 1113, 1707, 1809, 1821
FspBI	CTAG	4 cut(s)	347, 1451, 1563, 1703
GlaI	GCGC	3 cut(s)	163, 386, 1250
GluI	GCNGC	9 cut(s)	216, 229, 258, 1028, 1086, 1113, 1707, 1809, 1821
GsaI	CCCAGC	2 cut(s)	1173, 1182
GsuI	CTGGAG	3 cut(s)	482, 1122, 1692
HaeIII	GGCC	4 cut(s)	685, 1281, 1617, 1718
HapII	CCGG	3 cut(s)	401, 1644, 1924
HgaI	GACGC	2 cut(s)	627, 1255
HhaI	GCGC	3 cut(s)	164, 387, 1251
Hin1I	GRCGYC	2 cut(s)	240, 619
Hin1II	CATG	8 cut(s)	766, 828, 944, 952, 1340, 1346, 1691, 1900
Hin6I	GCGC	3 cut(s)	162, 385, 1249
HinP1I	GCGC	3 cut(s)	162, 385, 1249
HindIII	AAGCTT	2 cut(s)	49, 1527
HinfI	GANTC	4 cut(s)	143, 1271, 1304, 1854
HpaII	CCGG	3 cut(s)	401, 1644, 1924
HphI	GGTGA	6 cut(s)	160, 187, 260, 596, 734, 755
Hpy166II	GTNNAC	2 cut(s)	244, 795
Hpy188I	TCNGA	9 cut(s)	91, 452, 617, 645, 899, 1144, 1510, 1714, 1787
Hpy188III	TCNNGA	8 cut(s)	61, 425, 559, 665, 1208, 1337, 1671, 1865
Hpy8I	GTNNAC	2 cut(s)	244, 795
Hpy99I	CGWCG	1 cut(s)	224
HpyAV	CCTTC	2 cut(s)	868, 1468
HpyCH4III	ACNGT	6 cut(s)	98, 248, 580, 993, 1393, 1506
HpyCH4IV	ACGT	1 cut(s)	240
HpyCH4V	TGCA	5 cut(s)	442, 472, 1553, 1687, 1896
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1175, 1376, 1526, 1559, 1715, 1805, 1817, 1826
HpySE526I	ACGT	1 cut(s)	240
Hsp92I	GRCGYC	2 cut(s)	240, 619
Hsp92II	CATG	8 cut(s)	766, 828, 944, 952, 1340, 1346, 1691, 1900
HspAI	GCGC	3 cut(s)	162, 385, 1249
KpnI	GGTACC	1 cut(s)	1137
Kzo9I	GATC	5 cut(s)	325, 1212, 1257, 1357, 1579
LmnI	GCTCC	2 cut(s)	561, 1673
Lsp1109I	GCAGC	6 cut(s)	244, 1014, 1072, 1718, 1795, 1807
LweI	GCATC	4 cut(s)	244, 361, 429, 1357
MaeI	CTAG	4 cut(s)	347, 1451, 1563, 1703
MaeII	ACGT	1 cut(s)	240
MaeIII	GTNAC	4 cut(s)	71, 277, 712, 743
MalI	GATC	5 cut(s)	327, 1214, 1259, 1359, 1581
MboI	GATC	5 cut(s)	325, 1212, 1257, 1357, 1579
MboII	GAAGA	8 cut(s)	383, 442, 688, 1010, 1051, 1253, 1301, 1535
MflI	RGATCY	4 cut(s)	325, 1212, 1257, 1357
MhlI	GDGCHC	4 cut(s)	103, 897, 1149, 1336
MlsI	TGGCCA	1 cut(s)	1617
MluCI	AATT	9 cut(s)	551, 660, 768, 1284, 1315, 1380, 1554, 1677, 1744
MluNI	TGGCCA	1 cut(s)	1617
MlyI	GAGTC	2 cut(s)	152, 1848
MmeI	TCCRAC	3 cut(s)	599, 1515, 1647
Mox20I	TGGCCA	1 cut(s)	1617
MroXI	GAANNNNTTC	2 cut(s)	1308, 1792
MscI	TGGCCA	1 cut(s)	1617
MseI	TTAA	3 cut(s)	1320, 1353, 1383
MslI	CAYNNNNRTG	1 cut(s)	1341
Msp20I	TGGCCA	1 cut(s)	1617
MspA1I	CMGCKG	2 cut(s)	1520, 1799
MspI	CCGG	3 cut(s)	401, 1644, 1924
MspR9I	CCNGG	6 cut(s)	203, 286, 500, 1614, 1644, 1721
MvaI	CCWGG	5 cut(s)	203, 286, 500, 1614, 1721
MvnI	CGCG	2 cut(s)	387, 1249
MwoI	GCNNNNNNNGC	8 cut(s)	1175, 1376, 1526, 1559, 1715, 1805, 1817, 1826
NciI	CCSGG	1 cut(s)	1644
NdeI	CATATG	1 cut(s)	1831
NdeII	GATC	5 cut(s)	325, 1212, 1257, 1357, 1579
NheI	GCTAGC	1 cut(s)	346
NlaIII	CATG	8 cut(s)	766, 828, 944, 952, 1340, 1346, 1691, 1900
NlaIV	GGNNCC	3 cut(s)	19, 207, 1135
NmuCI	GTSAC	4 cut(s)	71, 277, 712, 743
NspI	RCATGY	2 cut(s)	828, 1900
PaeR7I	CTCGAG	1 cut(s)	665
PagI	TCATGA	1 cut(s)	1336
PcsI	WCGNNNNNNNCGW	2 cut(s)	228, 1858
PdmI	GAANNNNTTC	2 cut(s)	1308, 1792
PfeI	GAWTC	2 cut(s)	1271, 1304
PflMI	CCANNNNNTGG	1 cut(s)	581
PfoI	TCCNGGA	1 cut(s)	498
PinAI	ACCGGT	1 cut(s)	1923
PkrI	GCNGC	9 cut(s)	217, 230, 259, 1029, 1087, 1114, 1708, 1810, 1822
PleI	GAGTC	2 cut(s)	151, 1848
PpsI	GAGTC	2 cut(s)	151, 1848
Psp6I	CCWGG	5 cut(s)	201, 284, 498, 1612, 1719
PspEI	GGTNACC	1 cut(s)	743
PspFI	CCCAGC	2 cut(s)	1169, 1178
PspGI	CCWGG	5 cut(s)	201, 284, 498, 1612, 1719
PspN4I	GGNNCC	3 cut(s)	19, 207, 1135
PspPI	GGNCC	5 cut(s)	18, 205, 268, 1279, 1717
PstNI	CAGNNNCTG	2 cut(s)	871, 1712
PsuI	RGATCY	4 cut(s)	325, 1212, 1257, 1357
PvuII	CAGCTG	2 cut(s)	1520, 1799
RsaI	GTAC	5 cut(s)	311, 794, 990, 1135, 1516
RsaNI	GTAC	5 cut(s)	310, 793, 989, 1134, 1515
RseI	CAYNNNNRTG	1 cut(s)	1341
SaqAI	TTAA	3 cut(s)	1320, 1353, 1383
SatI	GCNGC	9 cut(s)	216, 229, 258, 1028, 1086, 1113, 1707, 1809, 1821
Sau3AI	GATC	5 cut(s)	325, 1212, 1257, 1357, 1579
Sau96I	GGNCC	5 cut(s)	18, 205, 268, 1279, 1717
SchI	GAGTC	2 cut(s)	152, 1848
ScrFI	CCNGG	6 cut(s)	203, 286, 500, 1614, 1644, 1721
SduI	GDGCHC	4 cut(s)	103, 897, 1149, 1336
SfaNI	GCATC	4 cut(s)	244, 361, 429, 1357
SfcI	CTRYAG	3 cut(s)	297, 1089, 1912
Sfr274I	CTCGAG	1 cut(s)	665
SinI	GGWCC	3 cut(s)	18, 205, 268
SlaI	CTCGAG	1 cut(s)	665
SmiMI	CAYNNNNRTG	1 cut(s)	1341
SmlI	CTYRAG	3 cut(s)	167, 665, 1208
SmoI	CTYRAG	3 cut(s)	167, 665, 1208
Sse9I	AATT	9 cut(s)	551, 660, 768, 1284, 1315, 1380, 1554, 1677, 1744
SsiI	CCGC	5 cut(s)	216, 228, 387, 1112, 1724
SspMI	CTAG	4 cut(s)	347, 1451, 1563, 1703
StyD4I	CCNGG	6 cut(s)	201, 284, 498, 1612, 1642, 1719
StyI	CCWWGG	3 cut(s)	748, 1328, 1824
TaaI	ACNGT	6 cut(s)	98, 248, 580, 993, 1393, 1506
TaiI	ACGT	1 cut(s)	243
TaqI	TCGA	5 cut(s)	546, 666, 1042, 1578, 1749
TaqII	GACCGA	1 cut(s)	506
TasI	AATT	9 cut(s)	551, 660, 768, 1284, 1315, 1380, 1554, 1677, 1744
TatI	WGTACW	3 cut(s)	792, 988, 1514
TauI	GCSGC	3 cut(s)	218, 231, 1115
TfiI	GAWTC	2 cut(s)	1271, 1304
Tru1I	TTAA	3 cut(s)	1320, 1353, 1383
Tru9I	TTAA	3 cut(s)	1320, 1353, 1383
TscAI	CASTG	4 cut(s)	310, 474, 492, 721
TseFI	GTSAC	4 cut(s)	71, 277, 712, 743
TseI	GCWGC	6 cut(s)	257, 1027, 1085, 1706, 1808, 1820
Tsp45I	GTSAC	4 cut(s)	71, 277, 712, 743
TspDTI	ATGAA	3 cut(s)	937, 1359, 1848
TspRI	CASTG	4 cut(s)	310, 474, 492, 721
Van91I	CCANNNNNTGG	1 cut(s)	581
VpaK11BI	GGWCC	3 cut(s)	18, 205, 268
XapI	RAATTY	4 cut(s)	660, 768, 1315, 1744
XceI	RCATGY	2 cut(s)	828, 1900
XhoI	CTCGAG	1 cut(s)	665
XmiI	GTMKAC	1 cut(s)	243
XmnI	GAANNNNTTC	2 cut(s)	1308, 1792
XspI	CTAG	4 cut(s)	347, 1451, 1563, 1703
ZraI	GACGTC	1 cut(s)	241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.