FvH4_1g06970

xaa-Pro dipeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017195
Physical Location & Seq
Forward (+)
3683838 .. 3689541
5704 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06970.t2

Sequence Viewer

Length: 1476 bp
ATGGCGTCGTCATCGTCGCTGACTCCTCCTCCAGTCCCGATCGAACTCCACGTCCGCAACCGCGAAAAGCTCCTCAGCTCTATTCGCCACCACCTCTCTCACTCCTCTCGGCCTCTCCACGGCTTCGTCTTACTCCAAGGAGGCGACGAACAAAATCGCTACGATACTGATCACGTCGATGTCTTCAGACAGGAGAGTTACTTTGCTTACTTGTTTGGAGTAAGAGAGCCTGGATTCTATGGTGCTATAGACATTGCGACTGGGAAATCTATTCTGTTTTCTCCTAGATTACCTGCTGAGTATGCTGTTTGGTCGGGGGAGATCAAGCCGTTATCATACTTTAAGGAGAGATATATGGTTAGTGTGGCATGCTATACCAATGAGATTACAAAAGTTTTACAAGATGAATGGCAGGCGTCCGGTAAACCTTTATTGTTTCTCTTGCATGGGCTCAACACTGACAGTGATAACTTCTCAAAACCAGCAGACTTTCAGGGGATTGATAACTTCGACACCGACTTGACTACCTTGCATCCAATTTTGACTGAATGCCGTGTTATAAAATCGGATCTGGAGCTTGCCCTTATCCAGTTTGCAAATGATATAAGCTCTGAAGCTCATGTCGAGGTAATGAGAAAAGCTAGGGCAGGCATGTATGAGTATCAGCTAGAAAGCATATTTCTTCACCACACCTACATGTATGGGGGTTGTAGACATTGCTCTTATACTTGTATTTGTGCTACTGGCGGAAATAGTGCTGTTCTCCATTATGGGCATGCAGCAGCTCCAAATGACAGGACCCTGGAAGATGGAGATTTGGCATTGCTTGATATGGGAGCTGAATACAATTTCTATGGGTCTGACATAACTTGTTCCTACCCTGTGAACGGAAAGTTTACCTCTGACCAATCCCTTATATACAATGCTGTTCTTGATGCTCACAATTCTGTCATATCAACACTGAAACCTGGAGTAAGCTGGGTTGATATGCATAAACTAGCAGAAAAGGTCATTCTTGAATCATTGAAGAGAGAGGGCATTCTGGATGGTAATGTAGACGATATGATGGTTGAACGACTGGGTGCTGTTTTCATGCCTCACGGGCTTGGCCATTTGCTTGGTATTGACACTCATGATCCTGGTGGCTACTTGAAGGGACCGGAGAGGATAAATGAACCTGGACTGAAGTCTTTGCGTACAACAAGAGAAATGCAAGAAGGGATGGTGATAACAGTGGAGCCTGGTTGCTACTTCATAGATGCTTTGTTGGTTCCAGCCATGGGAAGTCCAAATACTACAAAGTTCTTCAATCAGGCAGCAGTTGGGAGATTTAAAGGTTTTGGTGGAGTTCGAATTGAAAGCGATGTGCTCATCACAGCCAATGGTTGTAAGAACATGACAAAGGTTCCTCGGGAAATAGGCGAGATTGAAGCAGTAATGGCAGGGGCACCGTGGCCTCTTGACAGAGCCTCCTAA

Protein Analysis

492

Amino Acids

54.38

Weight (kDa)

5.49

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP_N PF05195 17 - 139 1.9e-22 Aminopeptidase P, N-terminal domain
Peptidase_M24 PF00557 194 - 459 5.7e-56 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29490
fragaria_vesca FvH4_1g06970 FvH4_1g06970
malus_domestica MD15G1203700.v1.1
prunus_persica Prupe.7G213700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0092921
rosa_laevigata RLG00000016347
rosa_multiflora Rmu_sc0000965.1_g000005
rosa_roxburghii Rroxscaffold_2G00148730
rosa_rugosa Rorug02G0030100
rosa_samantha Rh2AG076000 Rh2BG077300
rosa_wichuraiana Rw2G007010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 1447
AccI GTMKAC 2 cut(s) 712, 1056
AccII CGCG 1 cut(s) 63
AciI CCGC 3 cut(s) 55, 61, 747
AclWI GGATC 2 cut(s) 576, 1130
AcoI YGGCCR 1 cut(s) 1108
AcuI CTGAAG 3 cut(s) 169, 633, 1205
AcyI GRCGYC 2 cut(s) 5, 416
AfaI GTAC 1 cut(s) 1198
AfiI CCNNNNNNNGG 3 cut(s) 119, 887, 1280
AflIII ACRYGT 1 cut(s) 696
AgsI TTSAA 7 cut(s) 1019, 1027, 1073, 1153, 1309, 1358, 1430
AjiI CACGTC 2 cut(s) 52, 175
AjnI CCWGG 6 cut(s) 229, 801, 967, 1138, 1177, 1240
AloI GAACNNNNNNTCC 2 cut(s) 36, 68
Alw21I GWGCWC 1 cut(s) 1371
AlwI GGATC 2 cut(s) 576, 1130
Ama87I CYCGRG 1 cut(s) 1410
AoxI GGCC 3 cut(s) 110, 1108, 1454
ApeKI GCWGC 3 cut(s) 779, 782, 1316
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AspS9I GGNCC 2 cut(s) 798, 1157
AsuHPI GGTGA 2 cut(s) 677, 1237
AsuII TTCGAA 1 cut(s) 1351
AvaI CYCGRG 1 cut(s) 1410
AvaII GGWCC 2 cut(s) 798, 1157
BaeGI GKGCMC 1 cut(s) 1450
BalI TGGCCA 1 cut(s) 1110
BanI GGYRCC 1 cut(s) 1447
BanII GRGCYC 1 cut(s) 453
BarI GAAGNNNNNNTAC 2 cut(s) 1276, 1308
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 1371
BbvCI CCTCAGC 1 cut(s) 74
BbvI GCAGC 3 cut(s) 791, 794, 1328
BccI CCATC 4 cut(s) 803, 1040, 1060, 1216
BceAI ACGGC 3 cut(s) 136, 313, 537
BciT130I CCWGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
BclI TGATCA 1 cut(s) 169
BfaI CTAG 4 cut(s) 285, 642, 668, 998
BfmI CTRYAG 1 cut(s) 246
BfuAI ACCTGC 1 cut(s) 301
BglI GCCNNNNNGGC 1 cut(s) 1102
BisI GCNGC 3 cut(s) 780, 783, 1317
BlsI GCNGC 3 cut(s) 781, 784, 1318
Bme1390I CCNGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
Bme18I GGWCC 2 cut(s) 798, 1157
BmeT110I CYCGRG 1 cut(s) 1410
BmgBI CACGTC 2 cut(s) 52, 175
BmgT120I GGNCC 2 cut(s) 798, 1157
BmiI GGNNCC 6 cut(s) 800, 1158, 1239, 1272, 1407, 1449
BmrFI CCNGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
BmrI ACTGGG 2 cut(s) 270, 1088
BmsI GCATC 3 cut(s) 541, 925, 1249
BmuI ACTGGG 2 cut(s) 270, 1088
BoxI GACNNNNGTC 1 cut(s) 1186
BpiI GAAGAC 1 cut(s) 175
BpmI CTGGAG 3 cut(s) 15, 593, 990
Bpu10I CCTNAGC 1 cut(s) 74
Bpu14I TTCGAA 1 cut(s) 1351
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 2 cut(s) 5, 416
BsaJI CCNNGG 6 cut(s) 118, 136, 801, 1278, 1409, 1451
BsaWI WCCGGW 2 cut(s) 419, 1159
BsaXI ACNNNNNCTCC 3 cut(s) 13, 43, 1454
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 887, 1280
Bse1I ACTGG 5 cut(s) 32, 265, 589, 748, 1083
Bse3DI GCAATG 3 cut(s) 252, 715, 821
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
BseDI CCNNGG 6 cut(s) 118, 136, 801, 1278, 1409, 1451
BseGI GGATG 3 cut(s) 532, 1051, 1227
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 3 cut(s) 119, 887, 1280
BseMI GCAATG 3 cut(s) 252, 715, 821
BseMII CTCAG 2 cut(s) 88, 288
BseNI ACTGG 5 cut(s) 32, 265, 589, 748, 1083
BseRI GAGGAG 4 cut(s) 15, 18, 62, 94
BseSI GKGCMC 1 cut(s) 1450
BseXI GCAGC 3 cut(s) 791, 794, 1328
BseYI CCCAGC 1 cut(s) 978
Bsh1236I CGCG 1 cut(s) 63
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 3 cut(s) 112, 1110, 1456
BshNI GGYRCC 1 cut(s) 1447
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 1371
BsiHKCI CYCGRG 1 cut(s) 1410
BsiSI CCGG 2 cut(s) 420, 1160
BslFI GGGAC 2 cut(s) 20, 1170
BslI CCNNNNNNNGG 3 cut(s) 119, 887, 1280
BsmFI GGGAC 2 cut(s) 20, 1170
BsmI GAATGC 2 cut(s) 554, 1038
BsnI GGCC 3 cut(s) 112, 1110, 1456
BsoBI CYCGRG 1 cut(s) 1410
Bsp119I TTCGAA 1 cut(s) 1351
Bsp1286I GDGCHC 3 cut(s) 453, 1371, 1450
Bsp143I GATC 5 cut(s) 39, 169, 321, 568, 1135
Bsp19I CCATGG 1 cut(s) 1278
BspACI CCGC 3 cut(s) 55, 61, 747
BspANI GGCC 3 cut(s) 112, 1110, 1456
BspCNI CTCAG 2 cut(s) 87, 289
BspFNI CGCG 1 cut(s) 63
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 6 cut(s) 800, 1158, 1239, 1272, 1407, 1449
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 2 cut(s) 576, 1130
BspT104I TTCGAA 1 cut(s) 1351
BspT107I GGYRCC 1 cut(s) 1447
BsrDI GCAATG 3 cut(s) 252, 715, 821
BsrI ACTGG 5 cut(s) 32, 265, 589, 748, 1083
BssECI CCNNGG 6 cut(s) 118, 136, 801, 1278, 1409, 1451
BssMI GATC 5 cut(s) 39, 169, 321, 568, 1135
BssNI GRCGYC 2 cut(s) 5, 416
BssT1I CCWWGG 2 cut(s) 136, 1278
Bst2UI CCWGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
Bst4CI ACNGT 3 cut(s) 464, 1234, 1452
Bst6I CTCTTC 1 cut(s) 1022
BstACI GRCGYC 2 cut(s) 5, 416
BstBI TTCGAA 1 cut(s) 1351
BstC8I GCNNGC 5 cut(s) 370, 414, 579, 649, 777
BstDEI CTNAG 2 cut(s) 74, 297
BstDSI CCRYGG 3 cut(s) 118, 1278, 1451
BstF5I GGATG 3 cut(s) 532, 1051, 1227
BstFNI CGCG 1 cut(s) 63
BstKTI GATC 5 cut(s) 42, 172, 324, 571, 1138
BstMBI GATC 5 cut(s) 39, 169, 321, 568, 1135
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 4 cut(s) 84, 302, 1102, 1439
BstNI CCWGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
BstNSI RCATGY 4 cut(s) 372, 655, 700, 779
BstPAI GACNNNNGTC 1 cut(s) 1186
BstSCI CCNGG 6 cut(s) 229, 801, 967, 1138, 1177, 1240
BstSFI CTRYAG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 1450
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 3 cut(s) 791, 794, 1328
BstV2I GAAGAC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 568
BstXI CCANNNNNNTGG 1 cut(s) 1118
BstYI RGATCY 1 cut(s) 568
BsuRI GGCC 3 cut(s) 112, 1110, 1456
BtgI CCRYGG 3 cut(s) 118, 1278, 1451
BtgZI GCGATG 1 cut(s) 1377
BtrI CACGTC 2 cut(s) 52, 175
BtsCI GGATG 3 cut(s) 532, 1051, 1227
BtsIMutI CAGTG 4 cut(s) 456, 469, 959, 1239
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 5 cut(s) 370, 414, 579, 649, 777
CciI TCATGA 1 cut(s) 1132
Cfr13I GGNCC 2 cut(s) 798, 1157
CseI GACGC 1 cut(s) 405
Csp6I GTAC 1 cut(s) 1197
CviQI GTAC 1 cut(s) 1197
DdeI CTNAG 2 cut(s) 74, 297
DpnI GATC 5 cut(s) 41, 171, 323, 570, 1137
DpnII GATC 5 cut(s) 39, 169, 321, 568, 1135
DraI TTTAAA 1 cut(s) 1333
EaeI YGGCCR 1 cut(s) 1108
Eam1104I CTCTTC 1 cut(s) 1022
EarI CTCTTC 1 cut(s) 1022
EciI GGCGGA 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 136, 1278
Eco24I GRGCYC 1 cut(s) 453
Eco47I GGWCC 2 cut(s) 798, 1157
Eco57I CTGAAG 3 cut(s) 169, 633, 1205
Eco88I CYCGRG 1 cut(s) 1410
EcoO109I RGGNCCY 1 cut(s) 798
EcoRII CCWGG 6 cut(s) 229, 801, 967, 1138, 1177, 1240
EcoT14I CCWWGG 2 cut(s) 136, 1278
EcoT22I ATGCAT 1 cut(s) 993
EcoT38I GRGCYC 1 cut(s) 453
ErhI CCWWGG 2 cut(s) 136, 1278
FaqI GGGAC 2 cut(s) 20, 1170
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 2 cut(s) 712, 1056
Fnu4HI GCNGC 3 cut(s) 780, 783, 1317
FokI GGATG 3 cut(s) 519, 1058, 1234
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 3 cut(s) 780, 783, 1317
FspBI CTAG 4 cut(s) 285, 642, 668, 998
GluI GCNGC 3 cut(s) 780, 783, 1317
GsaI CCCAGC 1 cut(s) 982
GsuI CTGGAG 3 cut(s) 15, 593, 990
HaeIII GGCC 3 cut(s) 112, 1110, 1456
HapII CCGG 2 cut(s) 420, 1160
HgaI GACGC 1 cut(s) 405
Hin1I GRCGYC 2 cut(s) 5, 416
HinfI GANTC 3 cut(s) 22, 234, 1019
HpaII CCGG 2 cut(s) 420, 1160
HphI GGTGA 2 cut(s) 677, 1237
Hpy166II GTNNAC 5 cut(s) 425, 713, 886, 897, 1057
Hpy188I TCNGA 5 cut(s) 188, 568, 613, 862, 904
Hpy188III TCNNGA 8 cut(s) 37, 572, 932, 1016, 1043, 1133, 1412, 1460
Hpy8I GTNNAC 5 cut(s) 425, 713, 886, 897, 1057
Hpy99I CGWCG 4 cut(s) 10, 19, 149, 179
HpyAV CCTTC 2 cut(s) 1147, 1211
HpyCH4III ACNGT 3 cut(s) 464, 1234, 1452
HpyCH4IV ACGT 2 cut(s) 51, 174
HpyCH4V TGCA 6 cut(s) 445, 532, 596, 779, 991, 1213
HpyF10VI GCNNNNNNNGC 4 cut(s) 84, 302, 1102, 1439
HpyF3I CTNAG 2 cut(s) 74, 297
HpySE526I ACGT 2 cut(s) 51, 174
Hsp92I GRCGYC 2 cut(s) 5, 416
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 5 cut(s) 39, 169, 321, 568, 1135
LmnI GCTCC 5 cut(s) 75, 574, 790, 836, 1237
Lsp1109I GCAGC 3 cut(s) 791, 794, 1328
LweI GCATC 3 cut(s) 541, 925, 1249
MaeI CTAG 4 cut(s) 285, 642, 668, 998
MaeII ACGT 2 cut(s) 51, 174
MaeIII GTNAC 1 cut(s) 197
MalI GATC 5 cut(s) 41, 171, 323, 570, 1137
MboI GATC 5 cut(s) 39, 169, 321, 568, 1135
MboII GAAGA 5 cut(s) 175, 674, 818, 1039, 1297
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 3 cut(s) 453, 1371, 1450
MlsI TGGCCA 1 cut(s) 1110
MluCI AATT 4 cut(s) 537, 847, 943, 1353
MluNI TGGCCA 1 cut(s) 1110
MlyI GAGTC 1 cut(s) 16
Mox20I TGGCCA 1 cut(s) 1110
Mph1103I ATGCAT 1 cut(s) 993
MscI TGGCCA 1 cut(s) 1110
MseI TTAA 2 cut(s) 342, 1332
MslI CAYNNNNRTG 2 cut(s) 177, 695
Msp20I TGGCCA 1 cut(s) 1110
MspI CCGG 2 cut(s) 420, 1160
MspR9I CCNGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
Mva1269I GAATGC 2 cut(s) 554, 1038
MvaI CCWGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 4 cut(s) 84, 302, 1102, 1439
NcoI CCATGG 1 cut(s) 1278
NdeII GATC 5 cut(s) 39, 169, 321, 568, 1135
NlaIV GGNNCC 6 cut(s) 800, 1158, 1239, 1272, 1407, 1449
NmeAIII GCCGAG 1 cut(s) 88
NsiI ATGCAT 1 cut(s) 993
NspI RCATGY 4 cut(s) 372, 655, 700, 779
NspV TTCGAA 1 cut(s) 1351
PaeI GCATGC 2 cut(s) 372, 779
PagI TCATGA 1 cut(s) 1132
PciI ACATGT 1 cut(s) 696
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 2 cut(s) 554, 1038
PfeI GAWTC 2 cut(s) 234, 1019
PkrI GCNGC 3 cut(s) 781, 784, 1318
Ple19I CGATCG 1 cut(s) 42
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PpuMI RGGWCCY 1 cut(s) 798
PscI ACATGT 1 cut(s) 696
PshAI GACNNNNGTC 1 cut(s) 1186
PsiI TTATAA 1 cut(s) 560
Psp5II RGGWCCY 1 cut(s) 798
Psp6I CCWGG 6 cut(s) 229, 801, 967, 1138, 1177, 1240
PspFI CCCAGC 1 cut(s) 978
PspGI CCWGG 6 cut(s) 229, 801, 967, 1138, 1177, 1240
PspN4I GGNNCC 6 cut(s) 800, 1158, 1239, 1272, 1407, 1449
PspPI GGNCC 2 cut(s) 798, 1157
PspPPI RGGWCCY 1 cut(s) 798
PsuI RGATCY 1 cut(s) 568
PvuI CGATCG 1 cut(s) 42
RsaI GTAC 1 cut(s) 1198
RsaNI GTAC 1 cut(s) 1197
RseI CAYNNNNRTG 2 cut(s) 177, 695
SaqAI TTAA 2 cut(s) 342, 1332
SatI GCNGC 3 cut(s) 780, 783, 1317
Sau3AI GATC 5 cut(s) 39, 169, 321, 568, 1135
Sau96I GGNCC 2 cut(s) 798, 1157
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 6 cut(s) 231, 803, 969, 1140, 1179, 1242
SduI GDGCHC 3 cut(s) 453, 1371, 1450
SfaNI GCATC 3 cut(s) 541, 925, 1249
SfcI CTRYAG 1 cut(s) 246
SfuI TTCGAA 1 cut(s) 1351
SinI GGWCC 2 cut(s) 798, 1157
SmiMI CAYNNNNRTG 2 cut(s) 177, 695
SphI GCATGC 2 cut(s) 372, 779
Sse9I AATT 4 cut(s) 537, 847, 943, 1353
SsiI CCGC 3 cut(s) 55, 61, 747
SspMI CTAG 4 cut(s) 285, 642, 668, 998
StyD4I CCNGG 6 cut(s) 229, 801, 967, 1138, 1177, 1240
StyI CCWWGG 2 cut(s) 136, 1278
TaaI ACNGT 3 cut(s) 464, 1234, 1452
TaiI ACGT 2 cut(s) 54, 177
TaqI TCGA 5 cut(s) 42, 177, 510, 624, 1351
TasI AATT 4 cut(s) 537, 847, 943, 1353
TfiI GAWTC 2 cut(s) 234, 1019
Tru1I TTAA 2 cut(s) 342, 1332
Tru9I TTAA 2 cut(s) 342, 1332
TscAI CASTG 4 cut(s) 463, 469, 966, 1239
TseI GCWGC 3 cut(s) 779, 782, 1316
TspDTI ATGAA 4 cut(s) 420, 1081, 1188, 1243
TspGWI ACGGA 1 cut(s) 903
TspRI CASTG 4 cut(s) 463, 469, 966, 1239
VpaK11BI GGWCC 2 cut(s) 798, 1157
XceI RCATGY 4 cut(s) 372, 655, 700, 779
XmiI GTMKAC 2 cut(s) 712, 1056
XspI CTAG 4 cut(s) 285, 642, 668, 998
Zsp2I ATGCAT 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.