FvH4_1g07620

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013976
Physical Location & Seq
Forward (+)
4052059 .. 4056034
3976 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g07620.t1

Sequence Viewer

Length: 1653 bp
ATGCAGCTTTATTTCCCCTCCCCCTATATATACCTCTCTCTTTCTTCATCCCTTTATCTCCTGCCTGCCTCCCTTCTCTGCCTCTTTTTCTCATCTTCCATGGCTGAGAATTCTTCCTTATTCCTAACATACCTCATAGCCTTGTTCATCATGAGCCGCCTCATCTCCACCGTTGGCTACCGTCTCCGTGACGACCACGAAGCCATTAGACTAGCTTCCACTGACTACGGCCACATCCACAGCGAAAACCCTGCCGCCGTTCTCTACCCTTCTTCCATACACGACATTTCGTCACTCATCAAGTTCGCCAACAATGTCTCCGTGCCGTTTGGCGTGGCCGCGAAAGGCCAGGGCCACTCAACACGGGGACAGGCCATGGCTCGTAATGGAGTCGTGGTCGAAATGTCGTCGTTACGAAACCAACACGGCTCCTCCGGAATCGAAGTGGTTTCGACAACCAGCAAAGACGACGTGACGGTTTACTACGCGGACGTCGGAGGCGAGCAGCTCTGGGTCGATGTTCTGCATGCCACATTAGAACATGGGCTGTCGCCGGTGACGTGGACGGACTACTTGTACCTGACCGTCGGAGGGACGCTGTCGAACGCCGGAATCGGCGGACAGACGTTTCGGTTCGGCCCTCAGATCAGCAATGTCTACGAAATGGATGTTGTCACTGGAGAAGGGGATTTTGTGACTTGCTCCCCAAACAACAACGCAGAGCTTTTCTATGGGGTTCTTGGAGGGCTAGGTCAGTTTGGGGTTATATCCAGAGCCAGAATTGCACTAGAGCCAGCACCAACAAGGGTCAAGTGGGTGCGAATGCTTTACAGTGACTTCTCTGCGTTTTCCAGAGACCAAGAACGTTTAATCTCAATCAATGGGAGACAACAAAGCAATGCCCTCGATTATATAGAAGGCTCACTACTAATTAACCAGGGTCCCCCGAATAACTGGAGGGCTTCCTCATTTTTCCCTCAATCCTCACACAAAAAAATTATCTCCAGAGTAAACAAACATGGCATCATATATTGCCTCGAAGTCGTCAAATATTACGATCATCAAACTGAATCCACTGTGGACAAGGAGGTCCAAAATCTATTGAACGGGTTAAACTATCTTCCTGGTTTTAAGTTTGAAAATGATGCGTCGTATGTCGAATTTCTGAATCGAGTTCGAAGCGGAGAGCTGAAGCTTCAGTCAGAAGGACAATGGGATGTTCCTCATCCATGGCTTAATCTCTTTATTCCAAAGAGTCGCATCGCGGACTTTGATCAAGGAGTGTTCAGGGACATTGTTCTGAAGAGAAATATTACCACTGGACCTGTCCTCGTTTATCCCATGAACAGAAGCAAGTGGAATGAGAAAATGTCGGCAGTTGTACCCGAAGAAGATGTGTTTTACACCATAGGATTTTTGCATGCAACTGGGTTTGATGAATGGAAGGCATTTGATGAGCAGAACAAAGAGATATTAGAGTTTTGTGTTAAGGCTGGTATTGATGTTAAGCAGTATCTTCCAGACCACAAAACACAGCAAGGTTGGATGAAGCATTTTGGCTCGAAGTGGAAAACCTTCGAGAAAAGGAAAGCACTATTTGATCCCAAGAGAATACTTTCCCCTGGACAAAGAATTTTTCAGATTCAAGCATAA

Protein Analysis

551

Amino Acids

61.97

Weight (kDa)

6.58

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 84 - 235 3e-14 FAD binding domain
Cytokin-bind PF09265 267 - 546 1e-118 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56970 AT5G56970
fragaria_vesca FvH4_1g07620
malus_domestica MD15G1208300.v1.1
prunus_persica Prupe.7G208400_v2.0.a1 Prupe.7G208400_v2.0.a1
pyrus_communis pycom15g18450
rosa_chinensis RchiOBHm_Chr2g0093491
rosa_laevigata RLG00000016411
rosa_multiflora Rmu_sc0004423.1_g000008
rosa_roxburghii Rroxscaffold_2G00148040
rosa_rugosa Rorug02G0036700
rosa_samantha Rh2AG083200 Rh2BG083900 Rh2CG085900 Rh2DG082000
rosa_wichuraiana Rw2G006420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1088
AatII GACGTC 1 cut(s) 495
AccI GTMKAC 1 cut(s) 657
AccII CGCG 3 cut(s) 341, 488, 1265
AccIII TCCGGA 1 cut(s) 434
AciI CCGC 7 cut(s) 157, 255, 339, 488, 618, 1182, 1265
AclI AACGTT 1 cut(s) 865
AclWI GGATC 1 cut(s) 1595
AcoI YGGCCR 2 cut(s) 229, 336
AcsI RAATTY 3 cut(s) 109, 1160, 1632
AcuI CTGAAG 3 cut(s) 1181, 1211, 1322
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 2 cut(s) 578, 1383
AfiI CCNNNNNNNGG 1 cut(s) 591
AgsI TTSAA 3 cut(s) 1105, 1139, 1646
AhdI GACNNNNNGTC 1 cut(s) 289
AjiI CACGTC 2 cut(s) 472, 561
AjnI CCWGG 4 cut(s) 348, 936, 1123, 1621
AluBI AGCT 6 cut(s) 7, 215, 508, 724, 1189, 1195
AluI AGCT 6 cut(s) 7, 215, 508, 724, 1189, 1195
Alw26I GTCTC 4 cut(s) 188, 322, 849, 880
AlwI GGATC 1 cut(s) 1595
Aor13HI TCCGGA 1 cut(s) 434
AoxI GGCC 6 cut(s) 229, 336, 346, 352, 372, 637
ApeKI GCWGC 2 cut(s) 4, 505
ApoI RAATTY 3 cut(s) 109, 1160, 1632
ArsI GACNNNNNNTTYG 2 cut(s) 612, 644
Asp700I GAANNNNTTC 2 cut(s) 1574, 1615
AspS9I GGNCC 5 cut(s) 352, 638, 941, 1090, 1322
AsuHPI GGTGA 1 cut(s) 568
AsuII TTCGAA 1 cut(s) 1177
AvaII GGWCC 3 cut(s) 941, 1090, 1322
BarI GAAGNNNNNNTAC 2 cut(s) 909, 941
BbvI GCAGC 2 cut(s) 16, 517
BceAI ACGGC 4 cut(s) 242, 244, 310, 442
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 886, 920
BciT130I CCWGG 4 cut(s) 350, 938, 1125, 1623
BclI TGATCA 1 cut(s) 1273
BcoDI GTCTC 4 cut(s) 188, 322, 849, 880
BfaI CTAG 3 cut(s) 212, 749, 788
BisI GCNGC 5 cut(s) 5, 157, 255, 339, 506
BlsI GCNGC 5 cut(s) 6, 158, 256, 340, 507
Bme1390I CCNGG 4 cut(s) 350, 938, 1125, 1623
Bme18I GGWCC 3 cut(s) 941, 1090, 1322
BmeRI GACNNNNNGTC 1 cut(s) 289
BmgBI CACGTC 2 cut(s) 472, 561
BmgT120I GGNCC 5 cut(s) 352, 638, 941, 1090, 1322
BmiI GGNNCC 3 cut(s) 430, 942, 943
BmrFI CCNGG 4 cut(s) 350, 938, 1125, 1623
BmrI ACTGGG 1 cut(s) 1437
BmsI GCATC 3 cut(s) 1032, 1135, 1269
BmuI ACTGGG 1 cut(s) 1437
BpmI CTGGAG 3 cut(s) 699, 976, 988
Bpu14I TTCGAA 1 cut(s) 1177
BsaHI GRCGYC 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 849
BsaJI CCNNGG 6 cut(s) 99, 349, 375, 937, 1229, 1621
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 6 cut(s) 302, 332, 381, 411, 416, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 591
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 4 cut(s) 682, 959, 1324, 1432
Bse3DI GCAATG 2 cut(s) 658, 904
BseAI TCCGGA 1 cut(s) 434
BseBI CCWGG 4 cut(s) 350, 938, 1125, 1623
BseDI CCNNGG 6 cut(s) 99, 349, 375, 937, 1229, 1621
BseGI GGATG 6 cut(s) 47, 234, 673, 1222, 1225, 1551
BseLI CCNNNNNNNGG 1 cut(s) 591
BseMI GCAATG 2 cut(s) 658, 904
BseMII CTCAG 2 cut(s) 96, 656
BseNI ACTGG 4 cut(s) 682, 959, 1324, 1432
BseRI GAGGAG 1 cut(s) 421
BseXI GCAGC 2 cut(s) 16, 517
Bsh1236I CGCG 3 cut(s) 341, 488, 1265
BshFI GGCC 6 cut(s) 231, 338, 348, 354, 374, 639
BsiSI CCGG 3 cut(s) 435, 554, 609
BslFI GGGAC 4 cut(s) 381, 607, 927, 1304
BslI CCNNNNNNNGG 1 cut(s) 591
BsmAI GTCTC 4 cut(s) 188, 322, 849, 880
BsmBI CGTCTC 1 cut(s) 188
BsmFI GGGAC 4 cut(s) 381, 607, 927, 1304
BsmI GAATGC 1 cut(s) 828
BsnI GGCC 6 cut(s) 231, 338, 348, 354, 374, 639
Bso31I GGTCTC 1 cut(s) 849
Bsp119I TTCGAA 1 cut(s) 1177
Bsp13I TCCGGA 1 cut(s) 434
Bsp143I GATC 4 cut(s) 645, 1057, 1273, 1600
Bsp19I CCATGG 3 cut(s) 99, 375, 1229
BspACI CCGC 7 cut(s) 157, 255, 339, 488, 618, 1182, 1265
BspANI GGCC 6 cut(s) 231, 338, 348, 354, 374, 639
BspCNI CTCAG 2 cut(s) 97, 655
BspEI TCCGGA 1 cut(s) 434
BspFNI CGCG 3 cut(s) 341, 488, 1265
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 3 cut(s) 430, 942, 943
BspPI GGATC 1 cut(s) 1595
BspT104I TTCGAA 1 cut(s) 1177
BspTNI GGTCTC 1 cut(s) 849
BsrDI GCAATG 2 cut(s) 658, 904
BsrFI RCCGGY 1 cut(s) 553
BsrI ACTGG 4 cut(s) 682, 959, 1324, 1432
BssAI RCCGGY 1 cut(s) 553
BssECI CCNNGG 6 cut(s) 99, 349, 375, 937, 1229, 1621
BssMI GATC 4 cut(s) 645, 1057, 1273, 1600
BssNI GRCGYC 1 cut(s) 492
BssT1I CCWWGG 3 cut(s) 99, 375, 1229
Bst2UI CCWGG 4 cut(s) 350, 938, 1125, 1623
Bst4CI ACNGT 6 cut(s) 172, 182, 478, 586, 833, 1078
Bst6I CTCTTC 1 cut(s) 1298
BstACI GRCGYC 1 cut(s) 492
BstBI TTCGAA 1 cut(s) 1177
BstC8I GCNNGC 5 cut(s) 66, 503, 528, 795, 1422
BstDEI CTNAG 2 cut(s) 105, 642
BstDSI CCRYGG 3 cut(s) 99, 375, 1229
BstF5I GGATG 6 cut(s) 47, 234, 673, 1222, 1225, 1551
BstFNI CGCG 3 cut(s) 341, 488, 1265
BstKTI GATC 4 cut(s) 648, 1060, 1276, 1603
BstMAI GTCTC 4 cut(s) 188, 322, 849, 880
BstMBI GATC 4 cut(s) 645, 1057, 1273, 1600
BstMWI GCNNNNNNNGC 1 cut(s) 782
BstNI CCWGG 4 cut(s) 350, 938, 1125, 1623
BstNSI RCATGY 2 cut(s) 530, 1424
BstSCI CCNGG 4 cut(s) 348, 936, 1123, 1621
BstUI CGCG 3 cut(s) 341, 488, 1265
BstV1I GCAGC 2 cut(s) 16, 517
BsuRI GGCC 6 cut(s) 231, 338, 348, 354, 374, 639
BtgI CCRYGG 3 cut(s) 99, 375, 1229
BtgZI GCGATG 1 cut(s) 1246
BtrI CACGTC 2 cut(s) 472, 561
BtsCI GGATG 6 cut(s) 47, 234, 673, 1222, 1225, 1551
BtsIMutI CAGTG 5 cut(s) 219, 675, 838, 1074, 1317
Cac8I GCNNGC 5 cut(s) 66, 503, 528, 795, 1422
CciI TCATGA 1 cut(s) 150
Cfr10I RCCGGY 1 cut(s) 553
Cfr13I GGNCC 5 cut(s) 352, 638, 941, 1090, 1322
CseI GACGC 2 cut(s) 604, 1137
Csp6I GTAC 2 cut(s) 577, 1382
CviAII CATG 9 cut(s) 100, 151, 376, 527, 542, 1019, 1230, 1342, 1421
CviQI GTAC 2 cut(s) 577, 1382
DdeI CTNAG 2 cut(s) 105, 642
DpnI GATC 4 cut(s) 647, 1059, 1275, 1602
DpnII GATC 4 cut(s) 645, 1057, 1273, 1600
DrdI GACNNNNNNGTC 1 cut(s) 1088
DriI GACNNNNNGTC 1 cut(s) 289
DseDI GACNNNNNNGTC 1 cut(s) 1088
EaeI YGGCCR 2 cut(s) 229, 336
Eam1104I CTCTTC 1 cut(s) 1298
Eam1105I GACNNNNNGTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 1298
EciI GGCGGA 1 cut(s) 633
Eco130I CCWWGG 3 cut(s) 99, 375, 1229
Eco31I GGTCTC 1 cut(s) 849
Eco47I GGWCC 3 cut(s) 941, 1090, 1322
Eco57I CTGAAG 3 cut(s) 1181, 1211, 1322
EcoO109I RGGNCCY 1 cut(s) 941
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 4 cut(s) 348, 936, 1123, 1621
EcoT14I CCWWGG 3 cut(s) 99, 375, 1229
ErhI CCWWGG 3 cut(s) 99, 375, 1229
Esp3I CGTCTC 1 cut(s) 188
FaeI CATG 9 cut(s) 103, 154, 379, 530, 545, 1022, 1233, 1345, 1424
FaqI GGGAC 4 cut(s) 381, 607, 927, 1304
FatI CATG 9 cut(s) 99, 150, 375, 526, 541, 1018, 1229, 1341, 1420
FbaI TGATCA 1 cut(s) 1273
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 5 cut(s) 5, 157, 255, 339, 506
FokI GGATG 6 cut(s) 34, 221, 680, 1212, 1229, 1558
Fsp4HI GCNGC 5 cut(s) 5, 157, 255, 339, 506
FspBI CTAG 3 cut(s) 212, 749, 788
GluI GCNGC 5 cut(s) 5, 157, 255, 339, 506
GsuI CTGGAG 3 cut(s) 699, 976, 988
HaeIII GGCC 6 cut(s) 231, 338, 348, 354, 374, 639
HapII CCGG 3 cut(s) 435, 554, 609
HgaI GACGC 2 cut(s) 604, 1137
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 9 cut(s) 103, 154, 379, 530, 545, 1022, 1233, 1345, 1424
HindIII AAGCTT 1 cut(s) 1193
HinfI GANTC 7 cut(s) 390, 438, 612, 1070, 1168, 1255, 1642
HpaII CCGG 3 cut(s) 435, 554, 609
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 5 cut(s) 481, 564, 658, 1012, 1081
Hpy188I TCNGA 7 cut(s) 497, 590, 645, 1167, 1204, 1302, 1641
Hpy188III TCNNGA 7 cut(s) 151, 435, 771, 852, 1005, 1520, 1579
Hpy8I GTNNAC 5 cut(s) 481, 564, 658, 1012, 1081
Hpy99I CGWCG 5 cut(s) 412, 473, 497, 590, 1153
HpyAV CCTTC 7 cut(s) 83, 279, 677, 911, 1199, 1438, 1585
HpyCH4III ACNGT 6 cut(s) 172, 182, 478, 586, 833, 1078
HpyCH4IV ACGT 5 cut(s) 471, 492, 560, 626, 865
HpyCH4V TGCA 5 cut(s) 4, 526, 785, 1420, 1424
HpyF10VI GCNNNNNNNGC 1 cut(s) 782
HpyF3I CTNAG 2 cut(s) 105, 642
HpySE526I ACGT 5 cut(s) 471, 492, 560, 626, 865
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 9 cut(s) 103, 154, 379, 530, 545, 1022, 1233, 1345, 1424
KflI GGGWCCC 1 cut(s) 941
Kpn2I TCCGGA 1 cut(s) 434
Ksp22I TGATCA 1 cut(s) 1273
Kzo9I GATC 4 cut(s) 645, 1057, 1273, 1600
LmnI GCTCC 2 cut(s) 434, 707
Lsp1109I GCAGC 2 cut(s) 16, 517
LweI GCATC 3 cut(s) 1032, 1135, 1269
MaeI CTAG 3 cut(s) 212, 749, 788
MaeII ACGT 5 cut(s) 471, 492, 560, 626, 865
MaeIII GTNAC 8 cut(s) 188, 291, 411, 472, 556, 673, 694, 833
MalI GATC 4 cut(s) 647, 1059, 1275, 1602
MboI GATC 4 cut(s) 645, 1057, 1273, 1600
MboII GAAGA 9 cut(s) 36, 87, 105, 264, 1112, 1315, 1400, 1403, 1508
MluCI AATT 6 cut(s) 109, 780, 930, 996, 1160, 1632
MlyI GAGTC 2 cut(s) 399, 1264
MmeI TCCRAC 3 cut(s) 475, 568, 1523
MroI TCCGGA 1 cut(s) 434
MroXI GAANNNNTTC 2 cut(s) 1574, 1615
MseI TTAA 7 cut(s) 869, 933, 1112, 1131, 1236, 1488, 1506
MspI CCGG 3 cut(s) 435, 554, 609
MspR9I CCNGG 4 cut(s) 350, 938, 1125, 1623
Mva1269I GAATGC 1 cut(s) 828
MvaI CCWGG 4 cut(s) 350, 938, 1125, 1623
MvnI CGCG 3 cut(s) 341, 488, 1265
MwoI GCNNNNNNNGC 1 cut(s) 782
NcoI CCATGG 3 cut(s) 99, 375, 1229
NdeII GATC 4 cut(s) 645, 1057, 1273, 1600
NlaIII CATG 9 cut(s) 103, 154, 379, 530, 545, 1022, 1233, 1345, 1424
NlaIV GGNNCC 3 cut(s) 430, 942, 943
NmuCI GTSAC 7 cut(s) 188, 291, 472, 556, 673, 694, 833
NspI RCATGY 2 cut(s) 530, 1424
NspV TTCGAA 1 cut(s) 1177
PaeI GCATGC 2 cut(s) 530, 1424
PagI TCATGA 1 cut(s) 150
PcsI WCGNNNNNNNCGW 2 cut(s) 498, 557
PctI GAATGC 1 cut(s) 828
PdmI GAANNNNTTC 2 cut(s) 1574, 1615
PfeI GAWTC 5 cut(s) 438, 612, 1070, 1168, 1642
PflFI GACNNNGTC 1 cut(s) 598
PkrI GCNGC 5 cut(s) 6, 158, 256, 340, 507
PleI GAGTC 2 cut(s) 398, 1263
PpsI GAGTC 2 cut(s) 398, 1263
PpuMI RGGWCCY 1 cut(s) 941
Psp1406I AACGTT 1 cut(s) 865
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 4 cut(s) 348, 936, 1123, 1621
PspGI CCWGG 4 cut(s) 348, 936, 1123, 1621
PspN4I GGNNCC 3 cut(s) 430, 942, 943
PspPI GGNCC 5 cut(s) 352, 638, 941, 1090, 1322
PspPPI RGGWCCY 1 cut(s) 941
PsyI GACNNNGTC 1 cut(s) 598
RsaI GTAC 2 cut(s) 578, 1383
RsaNI GTAC 2 cut(s) 577, 1382
SaqAI TTAA 7 cut(s) 869, 933, 1112, 1131, 1236, 1488, 1506
SatI GCNGC 5 cut(s) 5, 157, 255, 339, 506
Sau3AI GATC 4 cut(s) 645, 1057, 1273, 1600
Sau96I GGNCC 5 cut(s) 352, 638, 941, 1090, 1322
SchI GAGTC 2 cut(s) 399, 1264
ScrFI CCNGG 4 cut(s) 350, 938, 1125, 1623
SfaNI GCATC 3 cut(s) 1032, 1135, 1269
SfuI TTCGAA 1 cut(s) 1177
SgrAI CRCCGGYG 1 cut(s) 553
SinI GGWCC 3 cut(s) 941, 1090, 1322
SphI GCATGC 2 cut(s) 530, 1424
Sse9I AATT 6 cut(s) 109, 780, 930, 996, 1160, 1632
SsiI CCGC 7 cut(s) 157, 255, 339, 488, 618, 1182, 1265
SspI AATATT 2 cut(s) 1052, 1312
SspMI CTAG 3 cut(s) 212, 749, 788
StyD4I CCNGG 4 cut(s) 348, 936, 1123, 1621
StyI CCWWGG 3 cut(s) 99, 375, 1229
TaaI ACNGT 6 cut(s) 172, 182, 478, 586, 833, 1078
TaiI ACGT 5 cut(s) 474, 495, 563, 629, 868
TasI AATT 6 cut(s) 109, 780, 930, 996, 1160, 1632
TauI GCSGC 3 cut(s) 159, 257, 341
TfiI GAWTC 5 cut(s) 438, 612, 1070, 1168, 1642
Tru1I TTAA 7 cut(s) 869, 933, 1112, 1131, 1236, 1488, 1506
Tru9I TTAA 7 cut(s) 869, 933, 1112, 1131, 1236, 1488, 1506
TscAI CASTG 5 cut(s) 226, 682, 838, 1081, 1324
TseFI GTSAC 7 cut(s) 188, 291, 472, 556, 673, 694, 833
TseI GCWGC 2 cut(s) 4, 505
Tsp45I GTSAC 7 cut(s) 188, 291, 472, 556, 673, 694, 833
TspDTI ATGAA 5 cut(s) 36, 136, 1358, 1452, 1562
TspGWI ACGGA 3 cut(s) 176, 310, 581
TspRI CASTG 5 cut(s) 226, 682, 838, 1081, 1324
Tth111I GACNNNGTC 1 cut(s) 598
VpaK11BI GGWCC 3 cut(s) 941, 1090, 1322
XapI RAATTY 3 cut(s) 109, 1160, 1632
XceI RCATGY 2 cut(s) 530, 1424
XmiI GTMKAC 1 cut(s) 657
XmnI GAANNNNTTC 2 cut(s) 1574, 1615
XspI CTAG 3 cut(s) 212, 749, 788
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.