Rh2DG082000

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013976
Physical Location & Seq
Forward (+)
6443944 .. 6447349
3406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG082000.1

Sequence Viewer

Length: 1557 bp
ATGACGTTTGCATCAGAAAGAAAAAGAGAGAAGGGAAAGAAAAGAAAGAATGATGAATCCAAGGCCTGTCCTTTGCGTATCTTGGAAAACCAATACGTGTTGAGCTTACGACGTCGTTCCCCACACAATGCCATACACATTTTAAAACGCCAAAGATTTCGTTCTCTGCCAATACAAGATTTTTACCCTCGTTCTCATGCAGCTTTATTTCCCTATATATACCTCTCTTCATCCTTTTATCTCCTACCTACCTACCCATTCCTTCTCTGCCTCACTTTCTCATTTTCAATGGCCAAGAATTCTTCTTCTTTCCCAACATACTTCATAGCCTTATTCATCATGAGCCGCCTGATCCCCACCATCGGATACCGCCTCCGTGACGACCCCGAAGCCATTAGATTAGCTTCCACTGATTACGGCCACATCCACAGCGAAAACCCAGCCGCCGTTCTCTACCCTTCTTCCATAAACGACATTTCGTCCCTCATCAAGTTCGCCAACAATGTTTCCGTGCCGTTTGGCGTGGCTGCGAAAGGCCAGGGCCACTCGACACGGGGACAGGCCATGGCTCGCAATGGGGTCGTGGTCGAAATGTCGTCCCTGCGAAACCACCTACCACAACACGGCTCCTCCGGAATCGAAGTAGTTTCAACCACCAACAAAGACGAAGTGAAGCTTTATTACGCGGACGTCGGAGGCGAGCAGCTCTGGGTCGATGTTCTGCATACCACATTAGAACATGGACTTTCACCGGTGACGTGGACGGACTACCTTTACTTGACCGTAGGAGGGACGCTGTCGAACGCCGGAATCGGCGGACAGACGTTTCGGTTCGGCCCTCAGATCAGCAATGTCTACGAAATGGATGTTGTCACTGGAGAAGGGGATTTTGTGACTTGCTCCCCAAACAACAACGCAGAGCTTTTCTATGGGGTTCTTGGAGGGCTAGGTCAGTTTGGGGTTATATCCAGAGCCAGAATTGCACTAGAGCCAGCACCAAAAAGGGTCAAGTGGGTGCGAATGCTTTACAGTGACTTCTCTGCGTTTTCCAGAGACCAGGAACGTTTAATCTCAATCAATGGGCGACAACAAAGCAATGCCCTTGATTATTTGGAAGGCTCAATACTAATTAACCAGGGTCCCCCGGATAATTGGAGGGCTTCCTCATTTTTTCCTCAATCCTCTCACAACAGAATTATTTCCAAAGTAAACAAACATGGGATCATATACTGCCTCGAAGTGGTCAAATATTATGACCATCACACTGAAACCACTGCGGATAAGGAGCTCCAAAATCTATTGAAAGGATTGAACTATCTTCCTGGATTTAAGTTTGAAAATGATGCGTCGTATGCCGAATTTCTGAATAGAGTTCGAAGCGGAGAGCTAAAGCTTCAGTCAGAAGGACTATGGGATGTCCCTCATCCGTGGCTTAATCTCTTTATTCCAAAAAGTCGCATCACGGACTTTGATCTTGGTGTGTTCAAGGACATTGTTCTGAAGAGAAATATTACCACCGGACCTGTCCTAGTTTACCCCATGAACAGGAGCAAGTAA

Protein Analysis

518

Amino Acids

58.52

Weight (kDa)

9.25

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 147 - 301 7.7e-14 FAD binding domain
Cytokin-bind PF09265 333 - 518 1.6e-70 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56970 AT5G56970
fragaria_vesca FvH4_1g07620
malus_domestica MD15G1208300.v1.1
prunus_persica Prupe.7G208400_v2.0.a1 Prupe.7G208400_v2.0.a1
pyrus_communis pycom15g18450
rosa_chinensis RchiOBHm_Chr2g0093491
rosa_laevigata RLG00000016411
rosa_multiflora Rmu_sc0004423.1_g000008
rosa_roxburghii Rroxscaffold_2G00148040
rosa_rugosa Rorug02G0036700
rosa_samantha Rh2AG083200 Rh2BG083900 Rh2CG085900 Rh2DG082000
rosa_wichuraiana Rw2G006420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 115, 693
AccI GTMKAC 1 cut(s) 855
AccII CGCG 1 cut(s) 686
AccIII TCCGGA 1 cut(s) 632
AciI CCGC 7 cut(s) 346, 370, 444, 686, 816, 1277, 1380
AclI AACGTT 1 cut(s) 1063
AclWI GGATC 2 cut(s) 346, 1229
AcoI YGGCCR 2 cut(s) 291, 418
AcsI RAATTY 2 cut(s) 298, 1358
AcuI CTGAAG 2 cut(s) 1379, 1520
AcyI GRCGYC 2 cut(s) 112, 690
AfiI CCNNNNNNNGG 5 cut(s) 362, 623, 789, 1240, 1545
AflIII ACRYGT 1 cut(s) 96
AgeI ACCGGT 1 cut(s) 751
AgsI TTSAA 6 cut(s) 288, 651, 1303, 1312, 1337, 1486
AhdI GACNNNNNGTC 1 cut(s) 478
AjiI CACGTC 1 cut(s) 759
AjnI CCWGG 4 cut(s) 537, 1056, 1134, 1321
AluBI AGCT 9 cut(s) 105, 203, 404, 676, 706, 922, 1288, 1387, 1393
AluI AGCT 9 cut(s) 105, 203, 404, 676, 706, 922, 1288, 1387, 1393
Alw21I GWGCWC 1 cut(s) 1290
Alw26I GTCTC 1 cut(s) 1047
AlwI GGATC 2 cut(s) 346, 1229
Aor13HI TCCGGA 1 cut(s) 632
AoxI GGCC 7 cut(s) 63, 291, 418, 535, 541, 561, 835
ApeKI GCWGC 3 cut(s) 200, 527, 703
ApoI RAATTY 2 cut(s) 298, 1358
ArsI GACNNNNNNTTYG 2 cut(s) 810, 842
AsiGI ACCGGT 1 cut(s) 751
Asp700I GAANNNNTTC 1 cut(s) 1198
AspS9I GGNCC 4 cut(s) 541, 836, 1139, 1520
AsuC2I CCSGG 1 cut(s) 1145
AsuHPI GGTGA 2 cut(s) 741, 766
AsuII TTCGAA 1 cut(s) 1375
AvaII GGWCC 2 cut(s) 1139, 1520
BalI TGGCCA 1 cut(s) 293
BanII GRGCYC 1 cut(s) 1290
BarI GAAGNNNNNNTAC 4 cut(s) 665, 697, 1107, 1139
Bbv12I GWGCWC 1 cut(s) 1290
BbvI GCAGC 3 cut(s) 212, 514, 715
BccI CCATC 2 cut(s) 368, 1266
BceAI ACGGC 4 cut(s) 431, 433, 499, 640
BcgI CGANNNNNNTGC 2 cut(s) 562, 596
BciT130I CCWGG 4 cut(s) 539, 1058, 1136, 1323
BciVI GTATCC 1 cut(s) 359
BcnI CCSGG 1 cut(s) 1145
BcoDI GTCTC 1 cut(s) 1047
BfaI CTAG 3 cut(s) 947, 986, 1529
BfuI GTATCC 1 cut(s) 359
BisI GCNGC 5 cut(s) 201, 346, 444, 528, 704
BlsI GCNGC 5 cut(s) 202, 347, 445, 529, 705
Bme1390I CCNGG 5 cut(s) 539, 1058, 1136, 1145, 1323
Bme18I GGWCC 2 cut(s) 1139, 1520
BmeRI GACNNNNNGTC 1 cut(s) 478
BmgBI CACGTC 1 cut(s) 759
BmgT120I GGNCC 4 cut(s) 541, 836, 1139, 1520
BmiI GGNNCC 3 cut(s) 628, 1140, 1141
BmrFI CCNGG 5 cut(s) 539, 1058, 1136, 1145, 1323
BmsI GCATC 3 cut(s) 20, 1333, 1467
BpmI CTGGAG 1 cut(s) 897
Bpu14I TTCGAA 1 cut(s) 1375
BpuMI CCSGG 1 cut(s) 1145
BsaAI YACGTR 1 cut(s) 97
BsaHI GRCGYC 2 cut(s) 112, 690
BsaI GGTCTC 1 cut(s) 1047
BsaJI CCNNGG 6 cut(s) 60, 538, 564, 1135, 1143, 1427
BsaWI WCCGGW 3 cut(s) 632, 751, 1517
BsaXI ACNNNNNCTCC 2 cut(s) 614, 644
Bsc4I CCNNNNNNNGG 5 cut(s) 362, 623, 789, 1240, 1545
Bse118I RCCGGY 1 cut(s) 751
Bse1I ACTGG 1 cut(s) 880
Bse3DI GCAATG 3 cut(s) 580, 856, 1102
BseAI TCCGGA 1 cut(s) 632
BseBI CCWGG 4 cut(s) 539, 1058, 1136, 1323
BseDI CCNNGG 6 cut(s) 60, 538, 564, 1135, 1143, 1427
BseGI GGATG 5 cut(s) 230, 423, 871, 1420, 1423
BseLI CCNNNNNNNGG 5 cut(s) 362, 623, 789, 1240, 1545
BseMI GCAATG 3 cut(s) 580, 856, 1102
BseMII CTCAG 1 cut(s) 854
BseNI ACTGG 1 cut(s) 880
BseRI GAGGAG 1 cut(s) 619
BseXI GCAGC 3 cut(s) 212, 514, 715
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 686
BshFI GGCC 7 cut(s) 65, 293, 420, 537, 543, 563, 837
BshTI ACCGGT 1 cut(s) 751
BsiHKAI GWGCWC 1 cut(s) 1290
BsiSI CCGG 5 cut(s) 633, 752, 807, 1145, 1518
BslFI GGGAC 6 cut(s) 466, 570, 583, 805, 1125, 1403
BslI CCNNNNNNNGG 5 cut(s) 362, 623, 789, 1240, 1545
BsmAI GTCTC 1 cut(s) 1047
BsmFI GGGAC 6 cut(s) 466, 570, 583, 805, 1125, 1403
BsmI GAATGC 1 cut(s) 1026
BsnI GGCC 7 cut(s) 65, 293, 420, 537, 543, 563, 837
Bso31I GGTCTC 1 cut(s) 1047
Bsp119I TTCGAA 1 cut(s) 1375
Bsp1286I GDGCHC 1 cut(s) 1290
Bsp13I TCCGGA 1 cut(s) 632
Bsp143I GATC 4 cut(s) 351, 843, 1221, 1471
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 7 cut(s) 346, 370, 444, 686, 816, 1277, 1380
BspANI GGCC 7 cut(s) 65, 293, 420, 537, 543, 563, 837
BspCNI CTCAG 1 cut(s) 853
BspEI TCCGGA 1 cut(s) 632
BspFNI CGCG 1 cut(s) 686
BspHI TCATGA 1 cut(s) 339
BspLI GGNNCC 3 cut(s) 628, 1140, 1141
BspPI GGATC 2 cut(s) 346, 1229
BspT104I TTCGAA 1 cut(s) 1375
BspTNI GGTCTC 1 cut(s) 1047
BsrDI GCAATG 3 cut(s) 580, 856, 1102
BsrFI RCCGGY 1 cut(s) 751
BsrI ACTGG 1 cut(s) 880
BssAI RCCGGY 1 cut(s) 751
BssECI CCNNGG 6 cut(s) 60, 538, 564, 1135, 1143, 1427
BssMI GATC 4 cut(s) 351, 843, 1221, 1471
BssNI GRCGYC 2 cut(s) 112, 690
BssT1I CCWWGG 2 cut(s) 60, 564
Bst2UI CCWGG 4 cut(s) 539, 1058, 1136, 1323
Bst4CI ACNGT 2 cut(s) 784, 1031
Bst6I CTCTTC 2 cut(s) 232, 1496
BstACI GRCGYC 2 cut(s) 112, 690
BstBAI YACGTR 1 cut(s) 97
BstBI TTCGAA 1 cut(s) 1375
BstC8I GCNNGC 3 cut(s) 571, 701, 993
BstDEI CTNAG 1 cut(s) 840
BstDSI CCRYGG 2 cut(s) 564, 1427
BstF5I GGATG 5 cut(s) 230, 423, 871, 1420, 1423
BstFNI CGCG 1 cut(s) 686
BstKTI GATC 4 cut(s) 354, 846, 1224, 1474
BstMAI GTCTC 1 cut(s) 1047
BstMBI GATC 4 cut(s) 351, 843, 1221, 1471
BstMWI GCNNNNNNNGC 2 cut(s) 980, 1352
BstNI CCWGG 4 cut(s) 539, 1058, 1136, 1323
BstSCI CCNGG 5 cut(s) 537, 1056, 1134, 1143, 1321
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 3 cut(s) 212, 514, 715
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 7 cut(s) 65, 293, 420, 537, 543, 563, 837
BtgI CCRYGG 2 cut(s) 564, 1427
BtrI CACGTC 1 cut(s) 759
BtsCI GGATG 5 cut(s) 230, 423, 871, 1420, 1423
BtsI GCAGTG 1 cut(s) 1272
BtsIMutI CAGTG 5 cut(s) 408, 873, 1036, 1263, 1272
Cac8I GCNNGC 3 cut(s) 571, 701, 993
CciI TCATGA 1 cut(s) 339
Cfr10I RCCGGY 1 cut(s) 751
Cfr13I GGNCC 4 cut(s) 541, 836, 1139, 1520
CseI GACGC 2 cut(s) 802, 1335
CspAI ACCGGT 1 cut(s) 751
CviAII CATG 6 cut(s) 197, 340, 565, 740, 1217, 1540
DdeI CTNAG 1 cut(s) 840
DpnI GATC 4 cut(s) 353, 845, 1223, 1473
DpnII GATC 4 cut(s) 351, 843, 1221, 1471
DraI TTTAAA 1 cut(s) 144
DriI GACNNNNNGTC 1 cut(s) 478
EaeI YGGCCR 2 cut(s) 291, 418
Eam1104I CTCTTC 2 cut(s) 232, 1496
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 2 cut(s) 232, 1496
EciI GGCGGA 1 cut(s) 831
Ecl136II GAGCTC 1 cut(s) 1288
Eco130I CCWWGG 2 cut(s) 60, 564
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 1290
Eco31I GGTCTC 1 cut(s) 1047
Eco47I GGWCC 2 cut(s) 1139, 1520
Eco53kI GAGCTC 1 cut(s) 1288
Eco57I CTGAAG 2 cut(s) 1379, 1520
EcoICRI GAGCTC 1 cut(s) 1288
EcoO109I RGGNCCY 1 cut(s) 1139
EcoRI GAATTC 1 cut(s) 298
EcoRII CCWGG 4 cut(s) 537, 1056, 1134, 1321
EcoT14I CCWWGG 2 cut(s) 60, 564
EcoT38I GRGCYC 1 cut(s) 1290
ErhI CCWWGG 2 cut(s) 60, 564
FaeI CATG 6 cut(s) 200, 343, 568, 743, 1220, 1543
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 6 cut(s) 466, 570, 583, 805, 1125, 1403
FatI CATG 6 cut(s) 196, 339, 564, 739, 1216, 1539
FblI GTMKAC 1 cut(s) 855
Fnu4HI GCNGC 5 cut(s) 201, 346, 444, 528, 704
FokI GGATG 5 cut(s) 217, 410, 878, 1410, 1427
FriOI GRGCYC 1 cut(s) 1290
Fsp4HI GCNGC 5 cut(s) 201, 346, 444, 528, 704
FspBI CTAG 3 cut(s) 947, 986, 1529
GluI GCNGC 5 cut(s) 201, 346, 444, 528, 704
GsaI CCCAGC 1 cut(s) 443
GsuI CTGGAG 1 cut(s) 897
HaeIII GGCC 7 cut(s) 65, 293, 420, 537, 543, 563, 837
HapII CCGG 5 cut(s) 633, 752, 807, 1145, 1518
HgaI GACGC 2 cut(s) 802, 1335
Hin1I GRCGYC 2 cut(s) 112, 690
Hin1II CATG 6 cut(s) 200, 343, 568, 743, 1220, 1543
HindIII AAGCTT 2 cut(s) 674, 1391
HinfI GANTC 3 cut(s) 56, 636, 810
HpaII CCGG 5 cut(s) 633, 752, 807, 1145, 1518
HphI GGTGA 2 cut(s) 741, 766
Hpy166II GTNNAC 4 cut(s) 762, 856, 1210, 1534
Hpy188I TCNGA 7 cut(s) 16, 365, 695, 843, 1365, 1402, 1500
Hpy188III TCNNGA 4 cut(s) 340, 633, 969, 1050
Hpy8I GTNNAC 4 cut(s) 762, 856, 1210, 1534
Hpy99I CGWCG 4 cut(s) 114, 117, 695, 1351
HpyAV CCTTC 6 cut(s) 25, 272, 468, 875, 1109, 1397
HpyCH4III ACNGT 2 cut(s) 784, 1031
HpyCH4IV ACGT 7 cut(s) 5, 96, 112, 690, 758, 824, 1063
HpyCH4V TGCA 4 cut(s) 11, 200, 724, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 980, 1352
HpyF3I CTNAG 1 cut(s) 840
HpySE526I ACGT 7 cut(s) 5, 96, 112, 690, 758, 824, 1063
Hsp92I GRCGYC 2 cut(s) 112, 690
Hsp92II CATG 6 cut(s) 200, 343, 568, 743, 1220, 1543
KflI GGGWCCC 1 cut(s) 1139
Kpn2I TCCGGA 1 cut(s) 632
Kzo9I GATC 4 cut(s) 351, 843, 1221, 1471
LmnI GCTCC 5 cut(s) 632, 905, 1285, 1293, 1548
Lsp1109I GCAGC 3 cut(s) 212, 514, 715
LweI GCATC 3 cut(s) 20, 1333, 1467
MaeI CTAG 3 cut(s) 947, 986, 1529
MaeII ACGT 7 cut(s) 5, 96, 112, 690, 758, 824, 1063
MaeIII GTNAC 5 cut(s) 377, 754, 871, 892, 1031
MalI GATC 4 cut(s) 353, 845, 1223, 1473
MboI GATC 4 cut(s) 351, 843, 1221, 1471
MboII GAAGA 6 cut(s) 219, 294, 297, 453, 1310, 1513
MhlI GDGCHC 1 cut(s) 1290
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 6 cut(s) 298, 978, 1128, 1150, 1194, 1358
MluNI TGGCCA 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 673
Mox20I TGGCCA 1 cut(s) 293
MroI TCCGGA 1 cut(s) 632
MroXI GAANNNNTTC 1 cut(s) 1198
MscI TGGCCA 1 cut(s) 293
MseI TTAA 5 cut(s) 143, 1067, 1131, 1329, 1434
Msp20I TGGCCA 1 cut(s) 293
MspI CCGG 5 cut(s) 633, 752, 807, 1145, 1518
MspR9I CCNGG 5 cut(s) 539, 1058, 1136, 1145, 1323
Mva1269I GAATGC 1 cut(s) 1026
MvaI CCWGG 4 cut(s) 539, 1058, 1136, 1323
MvnI CGCG 1 cut(s) 686
MwoI GCNNNNNNNGC 2 cut(s) 980, 1352
NciI CCSGG 1 cut(s) 1145
NcoI CCATGG 1 cut(s) 564
NdeII GATC 4 cut(s) 351, 843, 1221, 1471
NlaIII CATG 6 cut(s) 200, 343, 568, 743, 1220, 1543
NlaIV GGNNCC 3 cut(s) 628, 1140, 1141
NmuCI GTSAC 5 cut(s) 377, 754, 871, 892, 1031
NspV TTCGAA 1 cut(s) 1375
PagI TCATGA 1 cut(s) 339
PceI AGGCCT 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PctI GAATGC 1 cut(s) 1026
PdmI GAANNNNTTC 1 cut(s) 1198
PfeI GAWTC 3 cut(s) 56, 636, 810
PflFI GACNNNGTC 1 cut(s) 796
PfoI TCCNGGA 1 cut(s) 1321
PinAI ACCGGT 1 cut(s) 751
PkrI GCNGC 5 cut(s) 202, 347, 445, 529, 705
Ppu21I YACGTR 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 1139
Psp124BI GAGCTC 1 cut(s) 1290
Psp1406I AACGTT 1 cut(s) 1063
Psp5II RGGWCCY 1 cut(s) 1139
Psp6I CCWGG 4 cut(s) 537, 1056, 1134, 1321
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 4 cut(s) 537, 1056, 1134, 1321
PspN4I GGNNCC 3 cut(s) 628, 1140, 1141
PspPI GGNCC 4 cut(s) 541, 836, 1139, 1520
PspPPI RGGWCCY 1 cut(s) 1139
PsyI GACNNNGTC 1 cut(s) 796
SacI GAGCTC 1 cut(s) 1290
SaqAI TTAA 5 cut(s) 143, 1067, 1131, 1329, 1434
SatI GCNGC 5 cut(s) 201, 346, 444, 528, 704
Sau3AI GATC 4 cut(s) 351, 843, 1221, 1471
Sau96I GGNCC 4 cut(s) 541, 836, 1139, 1520
ScrFI CCNGG 5 cut(s) 539, 1058, 1136, 1145, 1323
SduI GDGCHC 1 cut(s) 1290
SfaNI GCATC 3 cut(s) 20, 1333, 1467
SfuI TTCGAA 1 cut(s) 1375
SgrAI CRCCGGYG 1 cut(s) 751
SinI GGWCC 2 cut(s) 1139, 1520
Sse9I AATT 6 cut(s) 298, 978, 1128, 1150, 1194, 1358
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 7 cut(s) 346, 370, 444, 686, 816, 1277, 1380
SspI AATATT 2 cut(s) 1250, 1510
SspMI CTAG 3 cut(s) 947, 986, 1529
SstI GAGCTC 1 cut(s) 1290
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 5 cut(s) 537, 1056, 1134, 1143, 1321
StyI CCWWGG 2 cut(s) 60, 564
TaaI ACNGT 2 cut(s) 784, 1031
TaiI ACGT 7 cut(s) 8, 99, 115, 693, 761, 827, 1066
TaqI TCGA 7 cut(s) 548, 588, 639, 714, 800, 1236, 1375
TasI AATT 6 cut(s) 298, 978, 1128, 1150, 1194, 1358
TauI GCSGC 2 cut(s) 348, 446
TfiI GAWTC 3 cut(s) 56, 636, 810
Tru1I TTAA 5 cut(s) 143, 1067, 1131, 1329, 1434
Tru9I TTAA 5 cut(s) 143, 1067, 1131, 1329, 1434
TscAI CASTG 5 cut(s) 415, 880, 1036, 1270, 1279
TseFI GTSAC 5 cut(s) 377, 754, 871, 892, 1031
TseI GCWGC 3 cut(s) 200, 527, 703
Tsp45I GTSAC 5 cut(s) 377, 754, 871, 892, 1031
TspDTI ATGAA 5 cut(s) 69, 219, 313, 325, 1556
TspGWI ACGGA 5 cut(s) 365, 499, 779, 1416, 1478
TspRI CASTG 5 cut(s) 415, 880, 1036, 1270, 1279
Tth111I GACNNNGTC 1 cut(s) 796
VpaK11BI GGWCC 2 cut(s) 1139, 1520
XapI RAATTY 2 cut(s) 298, 1358
XmiI GTMKAC 1 cut(s) 855
XmnI GAANNNNTTC 1 cut(s) 1198
XspI CTAG 3 cut(s) 947, 986, 1529
ZraI GACGTC 2 cut(s) 113, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.